Biological culture dish for microbial agent

Abstract

The invention relates to the technical field of culture dishes, and discloses a biological culture dish for microbial agents, which comprises a mounting base, a culture dish is rotatably mounted at the top of the mounting base, a filter membrane is vertically and fixedly mounted at the center in the culture dish, and a plurality of filter holes only allowing microorganisms to pass through are formed in the filter membrane. The two sides of the filter membrane extend to the inner wall of the culture dish, and the filter membrane divides the interior of the culture dish into an area A and an area B which are bilaterally symmetrical. Through eccentric rotation and partition management, metabolites are dynamically removed, and local oxygen deficit and pH unbalance are avoided. Microorganisms are periodically migrated between an interval A and an interval B and are always in a clean culture environment, dissolved oxygen and nutrients are uniformly distributed, thalli are directionally driven to transfer by centrifugal force, and meanwhile, metabolites are intercepted and cleaned, so that the survival rate of the thalli and the yield of target products are remarkably improved, and the diffusion limitation of static culture is broken through.

Description

Technical Field

[0001] The present invention relates to the technical field of culture dishes, and specifically to a biological culture dish for microbial agents. Background Art

[0002] Through the design of a transparent material and a sealing cover, the culture dish realizes the physical isolation of the microbial flora from the external environment, effectively avoiding contamination by miscellaneous bacteria. Its transparent property supports non-invasive observation, facilitating real-time monitoring of colony morphology, growth rate, and metabolic activities (such as enzyme production, pigment secretion, etc.), providing a visual basis for the evaluation of the activity and function verification of the microbial agent.

[0003] For example, in Chinese Patent Publication No. CN222434461U, which relates to the technical field of culture dishes, a fixing ring is provided above the base. Several connecting rods are fixed to the bottom of the fixing ring, and the connecting rods are arranged corresponding to the inner culture dishes one by one and are fixed on the base; several fixing mechanisms are annularly distributed inside the fixing ring, and the fixing mechanisms are connected to the base, and the inner culture dishes are arranged inside the fixing mechanisms; each of the fixing mechanisms includes: a collar fixed to the inner wall of the fixing ring, a placement tray provided at the inner bottom of the collar, and the inner culture dish is placed on the placement tray; it is convenient to move the inner culture dishes collectively, reducing the moving frequency; it is convenient to pick up each inner culture dish individually and operate on each inner culture dish, improving the practicability.

[0004] However, when statically culturing microbial agents through a culture dish, metabolites of microorganisms will accumulate locally, such as EPS (a viscous matrix secreted by microorganisms for biofilm construction), calcium carbonate crystals (aerobic bacteria metabolize to produce CO2, which combines with Ca in the culture medium and deposits), etc., which will cover the microbial flora precipitated at the bottom of the culture dish. The EPS viscous matrix hinders the diffusion of dissolved oxygen, causing the bottom flora to enter anaerobic metabolism, while the CaCO3 crystals wear the cell wall and release intracellular enzymes to trigger autolysis. Traditional stirring methods can only achieve homogeneous mixing but cannot guide microorganisms to escape from the covered area directionally. 2+ Summary of the Invention

[0005] The purpose of the present invention is to provide a biological culture dish for microbial agents to solve at least one technical problem existing in the above-mentioned prior art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A biological culture dish for microbial agents, including an installation base, a culture dish is rotatably installed on the top of the installation base, a filter membrane is vertically installed at the exact center inside the culture dish, several filter holes that can only allow microorganisms to pass through are opened on the filter membrane, both sides of the filter membrane extend to the inner wall of the culture dish, and the filter membrane divides the interior of the culture dish into two symmetrically left and right regions A and B. A baffle that can be vertically slid and adjusted is installed on the outer wall of the filter membrane, and the baffle penetrates through the top of the culture dish;

[0007] It further includes a driving component installed at the bottom of the culture dish. The driving component can make the culture dish rotate eccentrically, generating an asymmetric centrifugal force inside the culture dish to push the microorganisms to complete directional migration in two regions.

[0008] It further includes a cleaning component. The cleaning component can complete the cleaning of the target area before the microorganisms complete the area migration.

[0009] Preferably, the driving component includes a rotating disc installed at the top of the installation base and capable of rotating. An installation straight groove is formed at the top of the rotating disc. A slider is slidably installed on the inner wall of the installation straight groove. The culture dish is fixedly installed on the top of the culture dish. A rotatable screw rod is further installed inside the installation straight groove. The screw rod penetrates through the outer wall of the slider, and the screw rod is threadedly connected to the penetration part of the slider.

[0010] Preferably, an installation bracket is fixedly installed at the top of the rotating disc. V-shaped limiting chutes are fixedly installed on the inner walls of both sides of the installation bracket. Limiting shafts capable of sliding in the corresponding V-shaped limiting chutes are installed on the outer walls of both sides where the baffle plate extends out of the top of the culture dish.

[0011] Preferably, air bags are fixedly installed on the inner walls of both sides of the installation straight groove. Both air bags are annular, and both air bags wrap around the outer wall of the screw rod. One sides of the two air bags away from the inner wall of the installation straight groove are respectively fixedly connected to the outer walls of both sides of the slider. Both air bags are provided with air inlets communicating with the outside. Two first connecting pipes capable of connecting to areas A and B of the inner wall of the culture dish are respectively fixed on the outer walls of both air bags. One-way valves are installed in both the first connecting pipe and the air inlet.

[0012] Preferably, the cleaning component includes a liquid accumulation cavity formed inside the rotating disc. The liquid accumulation cavity is filled with cleaning liquid. A second connecting pipe capable of communicating with the first connecting pipe is installed on the inner wall of the liquid accumulation cavity. The area where the first connecting pipe is connected to the second connecting pipe is set to be narrow and curved.

[0013] Preferably, a hard pipe inclined away from the center of the rotating disc is provided at the connection part of the second connecting pipe and the rotating disc. When the air bag is in an inflated state, it will cause the hose of the second connecting pipe to deform, closing the space between the hose of the second connecting pipe and the hard pipe.

[0014] Preferably, the inside of the air bag and the inside of the liquid accumulation cavity are also communicated through a connecting pipe, and a one-way valve is installed in the connecting pipe.

[0015] Preferably, a section of the outer wall of the screw rod wrapped by the air bag is set as a smooth area.

[0016] Preferably, a counterweight is installed inside the installation base.

[0017] Preferably, the petri dish consists of a petri dish body and two detachable top plates on its top.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] First, through eccentric rotation and zonal management, the present invention dynamically removes metabolites, avoiding local hypoxia and pH imbalance. Microorganisms migrate periodically between zones A and B, always in a cleaned culture environment. The dissolved oxygen and nutrients are evenly distributed. The centrifugal force drives the bacteria to transfer directionally, while the metabolites are intercepted and cleaned, significantly improving the survival rate of bacteria and the yield of target products, breaking through the diffusion limitation of static culture.

[0020] Second, the present invention drives a slider to squeeze an airbag by a motor to inject high-pressure air into zone B to form an air cushion buffer layer. Under the action of eccentric centrifugal force, the microorganisms in zone A migrate efficiently to zone B through the inclined channels of the deformable filter membrane. At the same time, the high-pressure air temporarily blocks the microorganisms, winning time for cleaning zone B. After the migration is completed, the air pressure balance causes the filter membrane to reset, and with the mechanical action of the baffle pressing down, the metabolites (such as EPS) on the surface of the filter membrane are completely peeled off, avoiding blockage. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a perspective view of the present invention;

[0022] Figure 2 is a front cross-sectional view of the present invention;

[0023] Figure 3 is a three-dimensional cross-sectional view of the present invention;

[0024] Figure 4 is a front cross-sectional view of the petri dish of the present invention after offset;

[0025] Figure 5 is a top cross-sectional view of the present invention;

[0026] Figure 6 is a schematic diagram of the connection between the first connecting pipe and the second connecting pipe in the present invention;

[0027] Figure 7 is a schematic diagram of the change of filter holes after the filter membrane is bent in the present invention;

[0028] Figure 8 is a three-dimensional exploded cross-sectional view of the petri dish of the present invention;

[0029] Figure 9 is a three-dimensional cross-sectional view of the rotating disc in the present invention.

[0030] In the figure: 1, culture dish; 2, rotating disc; 3, mounting base; 4, mounting straight groove; 5, airbag; 6, mounting bracket; 7, V-shaped limit chute; 8, filter membrane; 9, baffle; 10, slider; 11, screw; 12, limit shaft; 13, top plate; 14, filter hole; 15, first connecting pipe; 16, second connecting pipe; 17, liquid accumulation cavity. Specific implementation mode

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

[0032] Please refer to Figures 1 to 9 , the present invention provides a technical solution: a biological culture dish for microbial inoculant, including a mounting base 3, a culture dish 1 is rotatably mounted on the top of the mounting base 3, a filter membrane 8 is vertically installed at the center of the inner part of the culture dish 1, and a number of filter holes 14 that can only allow microorganisms to pass through are opened on the filter membrane 8. Both sides of the filter membrane 8 extend to the inner wall of the culture dish 1, and the filter membrane 8 divides the inner part of the culture dish 1 into two symmetrically left and right regions A and B. A baffle 9 that can be vertically slid and adjusted is installed on the outer wall of the filter membrane 8, and the baffle 9 penetrates the top of the culture dish 1;

[0033] It also includes a driving component installed at the bottom of the culture dish 1. The driving component can eccentrically rotate the culture dish 1 to generate an asymmetric centrifugal force inside the culture dish 1, and push the microorganisms to complete directional migration in the two regions;

[0034] It also includes a cleaning component, and the cleaning component can clean the target area before the microorganisms complete the area migration.

[0035] Refer to Figures 1 to 4 , when culturing some microorganisms that need to be static, the metabolites of the microorganisms will accumulate at the bottom of the culture dish 1, which will not only cover the microorganisms precipitated at the bottom, prevent the diffusion of dissolved oxygen, and make them enter anaerobic metabolism, but also produce tiny crystals such as calcium carbonate, which may cause mechanical damage to the microorganisms. In addition, the lactic acid secreted by the microorganisms accumulates at the bottom, which will reduce the local PH to the optimal PH range of the microorganisms, thus significantly inhibiting the growth rate of the microorganisms and reducing the survival rate of the microorganisms to a certain extent.

[0036] Therefore, the interior of the culture dish 1 is divided into two regions, A and B, by the filter membrane 8. Region A is enclosed by the left wing of the filter membrane 8 and the left half-wall of the culture dish 1, and vice versa for Region B. In the initial stage, Region A serves as the culture area, and Region B serves as the cleaning area. Moreover, the baffle 9 is at the bottommost position, completely separating Region A and Region B. Initially, microorganisms are placed in Region A for cultivation. When the preset time has passed, the baffle 9 is moved upward through an external structure, connecting Region A and Region B. The culture dish 1 is eccentrically rotated by the driving assembly, and the axis of rotation is in the direction close to Region A. When the culture dish 1 rotates, Region A is always at the outer high position of the centrifugal force field, while Region B is located at the inner low position. Therefore, under the action of centrifugal force, the microorganisms will gradually transfer from Region A to Region B through the filter holes 14, and the remaining metabolites will stay in Region A, thus enabling the microorganisms to migrate from Region A to Region B. When the migration is completed, the baffle 9 moves downward to seal the two regions. Region A then serves as the cleaning area, and Region B serves as the culture area.

[0037] When the preset time has passed again, the axis of rotation of the culture dish 1 approaches Region B, causing the microorganisms to transfer from Region B back to Region A. During the transfer process, the interior of Region A is cleaned by the cleaning assembly to dissolve the metabolites therein, providing a more comfortable culture environment for the microorganisms.

[0038] Through eccentric rotation and partition management, metabolites are dynamically removed, avoiding local hypoxia and pH imbalance. The microorganisms migrate periodically between Regions A and B, always being in a cleaned culture environment. The dissolved oxygen and nutrients are evenly distributed. The centrifugal force drives the bacteria to transfer directionally, while the metabolites are intercepted and cleaned, significantly improving the survival rate of the bacteria and the yield of the target product, and breaking through the diffusion limitation of static cultivation.

[0039] It should be noted that elastic sealing strips are installed at the edges where the baffle 9 contacts the culture dish 1 to prevent particles floating in the external air from entering the interior of the culture dish 1 when the baffle 9 moves, enhancing the sealing performance during the cultivation of microorganisms.

[0040] Furthermore, the driving assembly includes a rotatable rotating disk 2 installed on the top of the mounting base 3. An installation straight groove 4 is formed on the top of the rotating disk 2. A slider 10 is slidably installed on the inner wall of the installation straight groove 4. The culture dish 1 is fixedly installed on the top of the culture dish 1. A rotatable screw 11 is also installed inside the installation straight groove 4. The screw 11 passes through the outer wall of the slider 10, and the screw 11 is threadedly connected to the penetration part of the slider 10.

[0041] See Figure 2, a rotating disc 2 is rotatably installed on the top of the culture dish 1, and a motor for driving the rotation of the rotating disc 2 is installed inside the culture dish 1. When it is necessary to transfer microorganisms between two regions after a predetermined time, first, the motor fixed to the outer wall of the rotating disc 2 drives the screw 11 to rotate. When the screw 11 rotates, it can drive the slider 10 and the culture dish 1 to move in the direction of area B. Then, the motor is started to drive the rotation of the rotating disc 2. Since the rotation axis of the rotating disc 2 is the axis where the center of the rotating disc 2 is located, and the central axis of the culture dish 1 is far from the axis where the rotating disc 2 is located, the microorganisms inside the culture dish 1 will be subjected to centrifugal force and gradually move towards area B to complete the migration process.

[0042] Furthermore, an installation bracket 6 is fixedly installed on the top of the rotating disc 2. V-shaped limiting chutes 7 are fixedly installed on both inner walls of the installation bracket 6. Limiting shafts 12 that can slide in the corresponding V-shaped limiting chutes 7 are installed on both outer walls of the baffle 9 extending out of the top of the culture dish 1.

[0043] See Figure 2 and Figure 8 , a limiting shaft 12 that can slide inside the V-shaped limiting chute 7 is installed on the outer wall of the baffle 9. When it is necessary to perform directional migration of microorganisms between different regions, the motor fixed to the outer wall of the rotating disc 2 drives the screw 11 to rotate, so that the slider 10 and the culture dish 1 move in the direction of area B. At this time, the limiting shaft 12 will slide inside the V-shaped limiting chute 7, thereby driving the baffle 9 to move upward and gradually separating from the filter membrane 8, making area A and area B communicate (i.e., Figure 4 the state shown). Then, the motor is started to complete the eccentric rotation shown above, so that the microorganisms complete the migration process under the action of the eccentric force. After the migration is completed, the slider 10 and the culture dish 1 are driven to move back to their original positions by driving the screw 11 to rotate. At the same time, under the cooperation of the V-shaped limiting chute 7 and the limiting shaft 12, the baffle 9 will return to its original position, and the baffle 9 will close the filter holes 14 on the filter membrane 8 again to prevent microorganisms from transferring back to the clean area from the culture area during the cultivation process.

[0044] Furthermore, air bags 5 are fixedly installed on both inner walls of the installation straight groove 4. Both air bags 5 are annular, and both air bags 5 wrap around the outer wall of the screw 11. One side of both air bags 5 far from the inner wall of the installation straight groove 4 is fixedly connected to both outer walls of the slider 10 respectively. Both air bags 5 are provided with air inlets communicating with the outside. Two first connecting pipes 15 that can be connected to areas A and B on the inner wall of the culture dish 1 are respectively fixed on the outer walls of both air bags 5, and one-way valves are installed in both the first connecting pipes 15 and the air inlets.

[0045] See Figure 5 , air bags 5 are fixedly installed on both inner walls of the installation straight groove 4. When it is necessary to migrate microorganisms (taking Figure 5Taking the transfer from area A to area B as an example, the motor fixed on the outer wall of the rotating disc 2 drives the screw 11 to rotate, thereby driving the right side of the slider 10 to move. When the slider 10 moves to the right, the above-mentioned baffle 9 moves upward to connect area A and area B, and the slider 10 will squeeze the airbag 5 on the right side, so that the air in the airbag 5 is injected into area B inside the culture dish 1, making the inside of area B in a high-pressure state. During the process of eccentrically rotating the culture dish 1 by the motor, the centrifugal force will cause the microorganisms in area A to migrate to area B, and the high-pressure air in area B will penetrate into area A through the filter holes 14 on the filter membrane 8 to form an air cushion layer, offsetting part of the centrifugal force, reducing the impact speed of microorganisms, reducing shear damage, and the air flow makes the migration process smoother, avoiding the rupture of the bacterial cells due to sudden acceleration. Moreover, when the pressure is the highest at the initial stage, it can temporarily prevent the microorganisms from migrating from area A to area B, enabling the cleaning component to have enough time to clean the metabolites inside area B, avoiding the premature entry of microorganisms into area B to interfere with the cleaning process, ensuring that metabolites such as lactic acid and CaCO3 are degraded or dissolved, and preventing secondary pollution.

[0046] Among them, it is worth mentioning that under the action of the pressure difference at both ends of area A and area B, the filter membrane 8 will bend towards the direction close to area A. Refer to Figure 7 , when the filter membrane 8 bends towards the direction close to area A, the filter holes 14 will form inclined channels, and the filter holes 14 close to area A will expand, which can reduce the resistance for microorganisms to pass through, guiding the microorganisms to slide along the inclined plane towards area B under the action of the centrifugal force, avoiding disordered diffusion. Moreover, the bending deformation of the filter membrane 8 can absorb part of the centrifugal impact and reduce the impact speed of the bacterial cells; when the pressures in area A and area B are equal, the filter membrane 8 will return to the vertical state, and the filter holes 14 will return to the horizontal state again, generating a mechanical extrusion effect to strip the adhered extracellular polysaccharides (EPS), proteins and other metabolites, making them fall back to area A to avoid long-term accumulation and blocking of the filter holes 14.

[0047] In addition, it is worth noting that due to the plasticity of the filter membrane 8, simply through the change of pressure, it may not be possible to completely reset the filter membrane 8. When the microorganisms complete the migration, during the process of the screw 11 rotating to drive the slider 10 and the culture dish 1 to reset, the baffle 9 will descend again to seal the filter membrane 8, and during this process, the baffle 9 can make the filter membrane 8 return to the vertical state again, making the filter holes 14 return to the normal size to ensure the precise separation of the next migration. Moreover, when the baffle 9 presses down, it applies an additional pressure to the filter membrane 8 to completely scrape off the adhered extracellular polysaccharides (EPS) and protein residues back to area A, further cleaning the surface of the filter membrane 8 to prevent the filter holes 14 from being blocked.

[0048] Further, the cleaning assembly includes a liquid accumulation cavity 17 formed inside the rotating disc 2. The liquid accumulation cavity 17 is filled with cleaning liquid. A second connecting pipe 16 capable of communicating with the first connecting pipe 15 is installed on the inner wall of the liquid accumulation cavity 17, and the area where the first connecting pipe 15 is connected to the second connecting pipe 16 is set to be narrow and curved.

[0049] Further, a hard pipe inclined away from the center of the rotating disc 2 is provided at the connection between the second connecting pipe 16 and the rotating disc 2. When the airbag 5 is in the inflated state, the hose of the second connecting pipe 16 will be deformed, closing the space between the hose of the second connecting pipe 16 and the hard pipe.

[0050] Refer to Figure 6 and Figure 7 As can be seen from the above, when it is necessary to make the microorganisms migrate in areas A and B, the driving screw 11 is rotated, thereby driving the slider 10 to slide to complete the subsequent eccentric rotation process. During this process, the airbag 5 on the side of the pressurized cleaning cavity will be pressed. When the airbag 5 is squeezed, the air inside the airbag 5 will be injected into the cleaning cavity inside the culture dish 1 through the first connecting pipe 15. And during this process, when the air wall passes through the narrow and curved part of the first connecting pipe 15, the cross-sectional area of the fluid flow will decrease and the flow velocity will increase. According to Bernoulli's principle, the increase in flow velocity will significantly reduce the hydrostatic pressure of the fluid in this area, thereby forming a pressure difference between the narrow part and the inside of the second connecting pipe 16. This pressure difference will drive the liquid in the second connecting pipe 16 to be sucked into the first connecting pipe 15, similar to the suction effect of a Venturi tube (similar to scenarios such as sprayers and jet pumps that require mixing or transporting fluids), so that the cleaning liquid in the liquid accumulation cavity 17 enters the second connecting pipe 16 through the first connecting pipe 15 and enters the cleaning cavity inside the culture dish 1 together with the air to complete the cleaning of the cleaning area cavity inside the culture dish 1. And the injected air will keep the cleaning cavity in a negative pressure state for a period of time to prevent the microorganisms from transferring before the cleaning is completed, which may affect the microorganisms.

[0051] It should be noted that a hard pipe inclined away from the center of the rotating disc 2 is provided at the connection between the second connecting pipe 16 and the rotating disc 2. In the initial stage, since the culture dish 1 is at the center of the rotating disc 2, the first connecting pipe 15 and the second connecting pipe 16 will be relatively close to the culture dish 1. At this time, there will be a certain pulling force between the hose of the second connecting pipe 16 and the hard pipe, deforming the hose at the connection and closing the space between the hose and the hard pipe to prevent the cleaning liquid inside the liquid accumulation cavity 17 from leaking; Figure 5For example, when the slider 10 drives the culture dish 1 to move leftward, it will drive the second connecting pipe 16 and the first connecting pipe 15 to move leftward simultaneously, thereby gradually reducing the pulling force on the hose of the second connecting pipe 16, making the connection between its hose and the rigid pipe, so as to achieve the above-mentioned purpose of injecting the gas-liquid mixture into the cleaning chamber of the culture dish 1.

[0052] Furthermore, the interior of the airbag 5 and the interior of the liquid accumulation chamber 17 are also connected through a connecting pipe, and a one-way valve is installed in the connecting pipe.

[0053] As can be seen from the above, when the airbag 5 is squeezed, the gas inside it can bring the cleaning liquid in the liquid accumulation chamber 17 into the cleaning chamber inside the culture dish 1 through the Venturi effect when moving towards the cleaning box in the culture dish 1 through the first connecting pipe 15. During this process, a part of the gas in the first connecting pipe 15 will enter the interior of the liquid accumulation chamber 17 through the connecting pipe, so as to form a high-pressure state inside the liquid accumulation chamber 17, thereby increasing the pressure difference between the second connecting pipe 16 and the first connecting pipe 15, enabling the cleaning liquid in the liquid accumulation chamber 17 to enter the first connecting pipe 15 more easily and then enter the cleaning chamber inside the culture dish 1.

[0054] It is worth mentioning that a valve can also be added to the connecting pipe, and the amount of air entering the interior of the liquid accumulation chamber 17 can be controlled by controlling the valve to control the release of the cleaning liquid.

[0055] Furthermore, a section of the outer wall of the screw rod 11 wrapped by the airbag 5 is set as a smooth area.

[0056] The section of the outer wall of the screw rod 11 that fits with the airbag 5 is set as a smooth area, and the smooth surface eliminates the shearing force of the rough thread on the airbag 5, preventing the thread from damaging the airbag 5.

[0057] Furthermore, a counterweight is installed inside the mounting base 3.

[0058] The counterweight balances the inertial force generated during eccentric rotation, lowers the center of gravity of the overall device, ensures zero risk of overturning during high-speed centrifugation or accidental collision, and the inertia of the counterweight assists in starting and stopping the motor, reducing the power consumption of the motor.

[0059] Furthermore, the culture dish 1 is composed of a culture dish 1 body and two detachable top plates 13 on its top.

[0060] See Figure 8 , when the device needs to be used, by opening the two top plates 13 on the top of the culture dish 1, microorganisms are placed in one of the chambers for cultivation. And after opening the two top plates 13, it is more convenient to clean and replace the filter membrane 8.

[0061] The standard parts used in this embodiment can be directly purchased from the market. For the non-standard structural components described in the specification and drawings, they can also be directly processed without any doubt based on the existing common technical knowledge. At the same time, the connection methods of each component adopt the mature conventional means in the existing technology, and the machinery, parts and equipment all adopt the conventional models in the existing technology. Therefore, no specific description will be made here.

[0062] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biological culture dish for a microbial inoculant, comprising a mounting base (3), characterized in that: A culture dish (1) is rotatably installed on the top of the installation base (3). A filter membrane (8) is vertically and fixedly installed at the exact center inside the culture dish (1). A number of filter holes (14) that can only allow microorganisms to pass through are formed in the filter membrane (8). Both sides of the filter membrane (8) extend to the inner wall of the culture dish (1), and the filter membrane (8) divides the interior of the culture dish (1) into two symmetrically left and right regions A and B. A baffle (9) that can be vertically slid and adjusted is installed on the outer wall of the filter membrane (8), and the baffle (9) penetrates through the top of the culture dish (1). It further includes a driving component installed at the bottom of the culture dish (1). The driving component can eccentrically rotate the culture dish (1) to generate an asymmetric centrifugal force inside the culture dish (1) and push the microorganisms to complete directional migration in the two regions. It further includes a cleaning component. The cleaning component can complete the cleaning of the target region before the microorganisms complete the regional migration.

2. The biological culture dish for microbial inoculum according to claim 1, characterized in that: The driving component includes a rotatable rotating disk (2) installed on the top of the installation base (3). An installation straight groove (4) is formed on the top of the rotating disk (2). A slider (10) is slidably installed on the inner wall of the installation straight groove (4). The culture dish (1) is fixedly installed on the top of the culture dish (1). A rotatable screw rod (11) is further installed inside the installation straight groove (4). The screw rod (11) penetrates through the outer wall of the slider (10), and the screw rod (11) is threadedly connected to the penetration part of the slider (10).

3. The biological culture dish for microbial inoculum according to claim 2, characterized in that: An installation bracket (6) is fixedly installed on the top of the rotating disk (2). V-shaped limiting sliding grooves (7) are fixedly installed on both inner walls of the installation bracket (6). Limiting shafts (12) that can slide in the corresponding V-shaped limiting sliding grooves (7) are installed on both outer walls of the baffle (9) extending out of the top of the culture dish (1).

4. The biological culture dish for microbial inoculum according to claim 2, characterized in that: Air bags (5) are fixedly installed on both inner walls of the installation straight groove (4). Both of the two air bags (5) are annular, and both of the two air bags (5) wrap around the outer wall of the screw rod (11). One sides of the two air bags (5) far from the inner wall of the installation straight groove (4) are respectively fixedly connected to both outer walls of the slider (10). Both of the two air bags (5) are provided with air inlets communicating with the outside. Two first connecting pipes (15) that can be connected to areas A and B of the inner wall of the culture dish (1) are respectively fixed on the outer walls of the two air bags (5), and one-way valves are installed in both the first connecting pipes (15) and the air inlets.

5. The biological culture dish for microbial inoculum according to claim 4, characterized in that: The cleaning component includes a liquid accumulation cavity (17) formed inside the rotating disk (2). Cleaning liquid is contained inside the liquid accumulation cavity (17). A second connecting pipe (16) that can be connected to the first connecting pipe (15) is installed on the inner wall of the liquid accumulation cavity (17), and the area where the first connecting pipe (15) is connected to the second connecting pipe (16) is set to be narrow and curved.

6. The biological culture dish for microbial inoculum according to claim 5, characterized in that: A section of hard pipe inclined away from the center of the rotating disk (2) is arranged at the connection part of the second connecting pipe (16) and the rotating disk (2). When the air bag (5) is in an inflated state, it will cause the hose of the second connecting pipe (16) to deform, so that the hose of the second connecting pipe (16) and the hard pipe are sealed.

7. The biological culture dish for microbial inoculum according to claim 4, characterized in that: The interior of the airbag (5) and the interior of the liquid accumulation cavity (17) are also communicated through a connecting pipe, and a one-way valve is installed in the connecting pipe.

8. The biological culture dish for microbial inoculum according to claim 4, characterized in that: The outer wall of a section of the screw rod (11) wrapped by the airbag (5) is provided with a smooth area.

9. The biological culture dish for microbial inoculum according to any one of claims 1-8, characterized in that: A counterweight is installed inside the mounting base (3).

10. The biological culture dish for microbial inoculum according to any one of claims 1-8, characterized in that: The culture dish (1) is composed of a culture dish (1) body and two detachable top plates (13) at its top.

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