Portable ultramicro digital PCR (Polymerase Chain Reaction) detector

Abstract

The invention relates to the field of PCR (Polymerase Chain Reaction) detection, in particular to a portable ultramicro digital PCR detector, which comprises a detection component, a handheld PCR detector, a detection area arranged on the handheld PCR detector, four storage grooves arranged in the detection area and a hot cover arranged on the detection area, the leakage-proof part comprises a sealing assembly arranged on the hot cover, a replacement assembly arranged on the sealing assembly, a guide assembly arranged in the detection area and a one-way assembly arranged on the guide assembly; the display component comprises a light component arranged on the sealing component, an induction component arranged on the sealing component and a conductive component arranged on the replacement component, the device can automatically seal the centrifugal tube, is convenient to operate, can give out an alarm when the centrifugal tube is inclined greatly, and is convenient to use. The accuracy of an experiment result is prevented from being influenced by a small amount of liquid at the bottom.

Description

Technical Field

[0001] The present invention relates to the field of PCR detection, and in particular to a portable ultra-trace digital PCR detector. Background Art

[0002] PCR detection uses a segment of DNA as a template. With the participation of DNA polymerase and nucleotide substrates, this segment of DNA is amplified to a sufficient quantity for structural and functional analysis. During the PCR detection process, the commonly used device is an ultra-trace digital PCR detector. The commonly used ultra-trace digital PCR detectors include fixed and portable types. The portable ones are divided into handheld and non-handheld according to their sizes. Since handheld PCR detectors are more portable, they are more widely used.

[0003] During use, when performing PCR detection with a handheld ultra-trace digital PCR detector, first place the sample to be detected into a centrifuge tube, then place the centrifuge tube into the detection area of the handheld ultra-trace digital PCR detector. There is a storage slot in the detection area for placing the centrifuge tube, and then cover the thermal cover and set the program for detection.

[0004] During detection, to avoid liquid leakage and to avoid affecting the experimental results due to long-term contact with oxygen, special lids are provided on each centrifuge tube. Before placing the centrifuge tube into the storage slot, the lids of each centrifuge tube need to be covered. This method is cumbersome to operate. Moreover, when the existing handheld ultra-trace digital PCR detector is detecting, it cannot detect the inclination of the centrifuge tube. When the inclination angle of the centrifuge tube is relatively large, the liquid at the bottom of the centrifuge tube will be less, which will affect the detection accuracy (the main reason is that less liquid at the bottom will result in less total DNA amplification in the detected liquid, thereby affecting the experimental results). Summary of the Invention

[0005] In view of the problem that before each centrifuge tube is placed into the storage slot, the lids of each centrifuge tube need to be covered, which is cumbersome to operate, and when the existing handheld ultra-trace digital PCR detector is detecting, it cannot detect the inclination of the centrifuge tube. When the inclination angle of the centrifuge tube is relatively large, the liquid at the bottom of the centrifuge tube will be less, which will affect the detection accuracy, the present invention is proposed.

[0006] Therefore, the object of the present invention is to provide an electrical component for a resolver.

[0007] To solve the above technical problems, the present invention provides the following technical solutions: It includes a detection component, which includes a handheld PCR detector, a detection area provided on the handheld PCR detector, four storage slots provided in the detection area, and a hot cover provided on the detection area; a leak prevention component, which includes a sealing component provided on the hot cover, a replacement component provided on the sealing component, a guiding component provided in the detection area, and a one-way component provided on the guiding component; a display component, which includes a brightening component provided on the sealing component, a sensing component provided on the sealing component, a conductive component provided on the replacement component; a switching component provided on the sensing component; and a backflow prevention component provided on the sealing component.

[0008] As a preferred solution of the portable ultra-trace digital PCR detector of the present invention, wherein: the sealing component includes a mounting plate provided on the lower side of the hot cover, and a sealing gasket is fixedly connected to the lower end surface of the mounting plate.

[0009] As a preferred solution of the portable ultra-trace digital PCR detector of the present invention, wherein: the replacement component includes a T-shaped groove provided in the hot cover, an L-shaped plate is hermetically slidably connected in the T-shaped groove, two sockets are provided on the L-shaped plate, a connecting plate is fixedly connected to the mounting plate, and a plug-in plate matched with the socket is fixedly connected to the connecting plate. Limit ports are provided on both the plug-in plate and the L-shaped plate, and a plug pin is provided in the limit port.

[0010] As a preferred solution of the portable ultra-trace digital PCR detector of the present invention, wherein: the guiding component includes a strip-shaped opening provided on the hot cover, a guiding rod fixedly connected to the L-shaped plate is provided in the strip-shaped opening, a horizontal groove is provided on the inner wall of the detection area, a vertical groove is communicated with the front end of the horizontal groove, an inclined groove is communicated with the vertical groove, the inclined groove is communicated with the horizontal groove, and the L-shaped plate is elastically connected to the inner wall of the T-shaped groove through a first spring.

[0011] As a preferred solution of the portable ultra-trace digital PCR detector of the present invention, wherein: the one-way component includes an extension groove provided in the inclined groove, a rotating shaft is rotatably connected in the extension groove, a baffle is fixedly connected to the rotating shaft, and a torsion spring is provided on the rotating shaft.

[0012] As a preferred solution of the portable ultra-trace digital PCR detector of the present invention, wherein: the brightening component includes four lamp posts fixedly connected to the mounting plate, four controllers matched with the lamp posts are fixedly connected to the mounting plate, the lamp posts are divided into multiple sections, and the colors of the multiple sections of the lamp posts are all different.

[0013] As a preferred embodiment of the portable ultra-micro digital PCR detector of the present invention, the induction component includes an annular groove provided on the mounting plate. A plurality of partition plates are fixedly connected in the annular groove, and the plurality of partition plates divide the annular groove into a plurality of induction areas. Humidity detection rods fixed to the inner wall of the annular groove are provided in the plurality of induction areas. Absorbent cotton is provided in the annular groove, and the humidity detection rods are inserted into the absorbent cotton.

[0014] As a preferred embodiment of the portable ultra-micro digital PCR detector of the present invention, the conductive component includes a conductive rod provided on the L-shaped plate. A receiving groove cooperating with the conductive rod is provided on the connecting plate, and a copper sheet cooperating with the conductive rod is fixedly connected to the inner wall of the receiving groove.

[0015] As a preferred embodiment of the portable ultra-micro digital PCR detector of the present invention, the switching component includes a notch provided on the sealing plate. A threaded column is fixedly connected to the mounting plate, a threaded sleeve is threadedly connected to the threaded column, and a disc cooperating with the absorbent cotton is fixedly connected to the threaded sleeve.

[0016] As a preferred embodiment of the portable ultra-micro digital PCR detector of the present invention, the anti-backflow component includes a plurality of outwardly expanding grooves provided on the sealing plate. Second springs are provided in the plurality of outwardly expanding grooves, and a splicing plate cooperating with the disc is fixedly connected to the second springs.

[0017] The beneficial effects of the portable ultra-micro digital PCR detector of the present invention are as follows: By providing a leak-proof component, even without a lid, the centrifuge tube can be sealed through the setting of the sealing plate, making the operation more convenient. At the same time, by providing a display component, when the centrifuge tube is tilted at a large angle, it can automatically sense and give a warning, and the degree of tilt can be understood through the light color of the light column, so as to abandon the current experiment and ensure the accuracy of the experimental detection. Thus, it solves the problem that before each centrifuge tube is placed in the storage slot, the lid of each centrifuge tube needs to be covered, which is a cumbersome operation, and the existing handheld ultra-micro digital PCR detector cannot detect the tilt of the centrifuge tube during detection. When the tilt angle of the centrifuge tube is large, the liquid at the bottom of the centrifuge tube will be less, which will affect the detection accuracy. It achieves the effects of more convenient operation during detection and more accurate detection results. Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 Schematic diagram of the external structure of a portable ultra - trace digital PCR detector.

[0020] Figure 2 Cross - sectional view of a portable ultra - trace digital PCR detector.

[0021] Figure 3 Schematic diagram of the external structure of the guiding component of a portable ultra - trace digital PCR detector.

[0022] Figure 4 For Figure 3 Enlarged schematic diagram of the structure at position A of

[0023] Figure 5 Exploded view of the replacement component of a portable ultra - trace digital PCR detector.

[0024] Figure 6 For Figure 5 Enlarged schematic diagram of the structure at position B of

[0025] Figure 7 Top view of the one - way component of a portable ultra - trace digital PCR detector.

[0026] Figure 8 Schematic diagram of the external structure of the replacement component of a portable ultra - trace digital PCR detector.

[0027] Figure 9 Schematic diagram of the external structure of the switching component of a portable ultra - trace digital PCR detector.

[0028] Figure 10 Cross - sectional view of the display component of a portable ultra - trace digital PCR detector.

[0029] In the figure: 100, detection component; 101, handheld PCR detector; 102, detection area; 103, storage tank; 104, thermal cover; 200, leak-proof component; 201, sealing component; 201a, mounting plate; 201b, gasket; 202, replacement component; 202a, T-shaped groove; 202b, L-shaped plate; 202c, socket; 202d, connecting plate; 202e, plug board; 202f, limiting port; 202g, bolt; 203, guiding component; 203a, strip-shaped opening; 203b, guiding rod; 203c, horizontal groove; 203d, vertical groove; 203e, inclined groove; 203f, first spring; 204, one-way component; 204a, extending groove; 204b, rotating shaft; 204c, baffle; 204d, torsion spring; 300, display component; 301, brightening component; 301a, lamp post; 301b, controller; 302, sensing component; 302a, annular groove; 302b, partition board; 302c, humidity detection rod; 302d, absorbent cotton; 303, conductive component; 303a, conductive rod; 303b, accommodating groove; 303c, copper sheet; 304, switching component; 304a, notch; 304b, threaded post; 304c, threaded sleeve; 304d, disc; 305, anti-backflow component; 305a, outward expanding groove; 305b, second spring; 305c, splicing board. Detailed implementation manners

[0030] To make the above objects, features, and advantages of the present invention more obvious and understandable, the detailed implementation manners of the present invention will be described in detail below with reference to the accompanying drawings of the specification.

[0031] Example 1, referring to Figures 1 to 8 , which is the first embodiment of the present invention. This embodiment provides a portable ultra-trace digital PCR detector, which can achieve the effect of avoiding sample leakage even when the handheld PCR detector 101 is tilted during handheld monitoring. It includes a detection component 100, including a handheld PCR detector 101, a detection area 102 provided on the handheld PCR detector 101, four storage tanks 103 provided in the detection area 102, and a thermal cover 104 provided on the detection area 102; a leak-proof component 200, including a sealing component 201 provided on the thermal cover 104, a replacement component 202 provided on the sealing component 201, a guiding component 203 provided in the detection area 102, and a one-way component 204 provided on the guiding component 203; a display component 300, including a brightening component 301 provided on the sealing component 201, a sensing component 302 provided on the sealing component 201, a conductive component 303 provided on the replacement component 202; a switching component 304 provided on the sensing component 302; and an anti-backflow component 305 provided on the sealing component 201.

[0032] Specifically, the handheld PCR detector 101 is provided with buttons and a display screen, capable of setting detection time, detection temperature, etc. There are sliding rails on the inner wall of the detection area 102 here to guide the thermal cover 104. The thermal cover 104 is pushed to open or close. After the first push, it can remain in the open state, and it will automatically close when pushed again. The above is the prior art and will not be elaborated here.

[0033] Furthermore, the closing assembly 201 includes a mounting plate 201a arranged on the lower side of the thermal cover 104, and a sealing gasket 201b is fixedly connected to the lower end surface of the mounting plate 201a; the replacement assembly 202 includes a T-shaped groove 202a arranged in the thermal cover 104, an L-shaped plate 202b is hermetically slidably connected in the T-shaped groove 202a, two sockets 202c are arranged on the L-shaped plate 202b, a connecting plate 202d is fixedly connected to the mounting plate 201a, and a plug plate 202e matched with the socket 202c is fixedly connected to the connecting plate 202d. Limit ports 202f are arranged on both the plug plate 202e and the L-shaped plate 202b, and a plug pin 202g is arranged in the limit port 202f.

[0034] Among them, the T-shaped groove 202a and the strip-shaped opening 203a here are staggered from the circuits in the thermal cover 104 and will not affect the use of the thermal cover 104. A damping is provided between the limit port 202f and the plug pin 202g, thereby preventing the plug pin 202g from automatically disengaging from the limit port 202f and causing the separation of the L-shaped plate 202b and the connecting plate 202d. The plug pin 202g here can be set in a T shape for the removal of the plug pin 202g.

[0035] Preferably, the guiding assembly 203 includes a strip-shaped opening 203a arranged on the thermal cover 104, a guiding rod 203b fixedly connected to the L-shaped plate 202b is arranged in the strip-shaped opening 203a, a horizontal groove 203c is arranged on the inner wall of the detection area 102, a vertical groove 203d is communicated with the front end of the horizontal groove 203c, an inclined groove 203e is communicated with the vertical groove 203d, and the inclined groove 203e is communicated with the horizontal groove 203c. The L-shaped plate 202b is elastically connected to the inner wall of the T-shaped groove 202a through a first spring 203f; the one-way assembly 204 includes an extension groove 204a arranged in the inclined groove 203e, a rotating shaft 204b is rotatably connected in the extension groove 204a, a baffle 204c is fixedly connected to the rotating shaft 204b, and a torsion spring 204d is arranged on the rotating shaft 204b.

[0036] It should be noted that the first spring 203f is in an extended state. The horizontal groove 203c is located below the sliding rail here. When the guiding rod 203b moves, it will not get stuck with the sliding rail. The lower end of the baffle 204c abuts against the inner wall of the extension groove 204a, so that the baffle 204c can only deflect upward and cannot deflect downward.

[0037] In use, first, the hot cover 104 is in an open state. At this time, the first spring 203f is in an extended state. Then, the sample to be detected is placed into the storage groove 103 through a centrifuge tube (without a cover). Then, the hot cover 104 is covered, and the hot cover 104 moves forward. During this process, the guide rod 203b first slides in the horizontal groove 203c. At this time, there is a distance between the sealing plate and the upper end of the centrifuge tube. Therefore, during the movement of the sealing plate, even if the centrifuge tube is not fully placed in position, it will not cause the sealing plate to collide with the centrifuge tube during movement, resulting in further tilting of the centrifuge tube and the outflow of the sample in the centrifuge tube. When the guide rod 203b moves to the connection between the horizontal groove 203c and the inclined groove 203e, since the lower end of the baffle 204c abuts against the inner wall of the extension groove 204a, the baffle 204c can only deflect upward and cannot deflect downward. Therefore, the guide rod 203b does not enter the inclined groove 203e but continues to move forward to the connection between the horizontal groove 203c and the vertical groove 203d. At this time, under the action of the first spring 203f, the L-shaped plate 202b resets, causing the connecting plate 202d to reset, and the sealing plate presses on the upper end of the centrifuge tube to seal the centrifuge tube. There is no need to specifically set a cover plate for sealing. And after the detection is completed, when the hot cover 104 is opened, the guide rod 203b slides in the inclined groove 203e. At this time, the sealing plate slowly moves backward and separates from the centrifuge tube. When the guide rod 203b moves to abut against the lower end surface of the baffle 204c, continued movement can push the baffle 204c to flip upward. Finally, the guide rod 203b moves into the horizontal groove 203c. At this time, the first spring 203f extends for the next use. It should be noted that although this method increases the contact time between the sample and oxygen, since an oxygen scavenger is added to the sample and its reaction also takes time (when using a covered one, it also takes time to cover), here, as long as the sample preparation is controlled within a certain time, it is possible to avoid the influence of sample contact with oxygen on the experimental accuracy. And generally, when the handheld PCR detector 101 is detecting, usually two samples are detected at a time, so the waiting time is less, further avoiding the influence of sample contact with oxygen on the experimental accuracy.

[0038] In summary, by setting the leak-proof component 200, when performing PCR detection of samples, only need to place the sample into the storage groove 103 and then cover the hot cover 104, then the sealing of multiple centrifuge tubes can be carried out. On the one hand, the centrifuge tube lids can be omitted, and on the other hand, there is no need to open and close the lids, saving the detection time.

[0039] Example 2, refer to Figures 1 to 10, which is the second embodiment of the present invention. Different from the previous embodiment, this embodiment provides a display component 300 of a portable ultra-micro digital PCR detector, which solves the problem that the sample detection effect is affected when the handheld PCR detector 101 is tilted too much. It includes a bright light component 301, which includes four lamp posts 301a fixedly connected to the mounting plate 201a. Four controllers 301b cooperating with the lamp posts 301a are fixedly connected to the mounting plate 201a. The lamp posts 301a are divided into multiple sections, and the colors of the multiple sections of lamp posts 301a are all different. The sensing component 302 includes an annular groove 302a arranged on the mounting plate 201a. Multiple partition plates 302b are fixedly connected in the annular groove 302a. The multiple partition plates 302b divide the annular groove 302a into multiple sensing areas. Humidity detection rods 302c fixed to the inner wall of the annular groove 302a are arranged in the multiple sensing areas. A water-absorbing cotton 302d is arranged in the annular groove 302a, and the humidity detection rods 302c are inserted into the water-absorbing cotton 302d.

[0040] Specifically, during detection, it is necessary to ensure that there is a certain amount of sample at the bottom of the centrifuge tube. When the sample is too small, it is easy to affect the detection result. The reason is generally that the centrifuge tube is tilted. Large detectors are fixed on the desktop or the ground and will not tilt, but the handheld PCR detector 101 is generally held by hand and is thus prone to tilting.

[0041] Furthermore, the conductive component 303 includes a conductive rod 303a arranged on the L-shaped plate 202b. A receiving groove 303b cooperating with the conductive rod 303a is arranged on the connecting plate 202d. A copper sheet 303c cooperating with the conductive rod 303a is fixedly connected to the inner wall of the receiving groove 303b; the switching component 304 includes a notch 304a arranged on the sealing plate. A threaded post 304b is fixedly connected to the mounting plate 201a. A threaded sleeve 304c is threadedly connected to the threaded post 304b. A disc 304d cooperating with the water-absorbing cotton 302d is fixedly connected to the threaded sleeve 304c.

[0042] It should be noted that the cooperation between the conductive rod 303a and the copper sheet 303c can provide power for the humidity detection rods 302c, the controllers 301b, and the lamp posts 301a. The setting of the water-absorbing cotton 302d will absorb the liquid in the sample. It is worth noting that when the water-absorbing cotton 302d can absorb the liquid, it means that the sample at the bottom of the centrifuge tube is less. At this time, the experiment needs to be restarted (because the detection is a continuous process, and it is uncertain when the liquid is less and the data will change greatly, resulting in experimental deviations). Therefore, even if the water-absorbing cotton 302d absorbs the liquid, it will not cause an impact.

[0043] During use, when detecting, since the handheld PCR detector 101 is in a handheld state and the human eye observes the display screen of the handheld PCR detector 101 more, it is inevitable that there will be a large inclination when pressing the button. When the inclination is large, the liquid at the bottom of the centrifuge tube becomes less. When the inclination angle is large enough that the liquid at the bottom of the centrifuge tube affects the experimental results, the liquid in the centrifuge tube will come into contact with the absorbent cotton 302d, and then the liquid will spread through the capillary force of the absorbent cotton 302d until it contacts the humidity detection rod 302c. At this time, the humidity detection rod 302c senses and transmits a signal to the controller 301b, causing the lamp beads to light up, and at the same time, it will be displayed on the display screen to stop the experiment in time. Here, the lamp column 301a is divided into multiple segments, each with a different color. Thus, according to the different humidity of the humidity detection rod 302c, the color of the lamp column 301a can be changed to reflect the degree of excessive inclination angle, thereby correcting the operation during use. When the absorbent cotton 302d needs to be replaced, just pull out the bolt 202g, then rotate it, and then separate the plug board 202e from the socket 202c, then the mounting plate 201a can be taken out. Then rotate the disc 304d so that the threaded sleeve 304c on the disc 304d is separated from the threaded rod, and the absorbent cotton 302d can be directly pulled out for replacement.

[0044] In summary, by setting the display component 300, it can automatically give a prompt when the inclination angle is too large, and the lamp column 301a is also set. According to the color of the lamp column 301a, the degree of inclination angle deviation can be judged, so that the operation method can be corrected, and the influence caused by less liquid at the bottom can be avoided, making the detection more accurate.

[0045] Example 3, referring to Figures 1 to 10 , which is the third embodiment of the present invention. Different from the previous embodiment, this embodiment provides an anti-backflow component 305 for the portable ultra-trace digital PCR detector, which solves the problem of how to avoid the absorbent cotton 302d dripping water into the detection area 102 after the absorbent cotton 302d absorbs water. It includes an anti-backflow component 305, which includes a plurality of outwardly expanding grooves 305a provided on the sealing plate. A second spring 305b is provided in each of the plurality of outwardly expanding grooves 305a, and a splicing plate 305c that cooperates with the disc 304d is fixedly connected to the second spring 305b. Specifically, the second spring 305b is connected with an electric wire, and the second spring 305b can be electrified. When the second spring 305b is electrified, the second spring 305b contracts. The reason is that after the second spring 305b is electrified, it is equivalent to an electrified spiral coil, and each adjacent coil attracts each other with opposite polarity, so the second spring 305b will contract.

[0046] In use, first, the second spring 305b is in the energized state and compressed. When the humidity detection rod 302c senses liquid, the controller 301b can also cut off the power supply of the second spring 305b. At this time, the second spring 305b resets, causing the splicing plate 305c to move towards the disc 304d. When the splicing plate 305c abuts against the side wall of the disc 304d, the lower end of the absorbent cotton 302d is sealed, so that when the movable sealing plate is moved, there will be no phenomenon that too much liquid on the absorbent cotton 302d drips into the detection area 102. It should be noted that the second spring 305b is set with a power-off delay, so that there is enough time for the tester to know from the display screen that the centrifuge tube is tilted, and then correct the handheld PCR detector 101 to avoid the splicing plate 305c passing through the liquid.

[0047] In summary, by setting the anti-backflow component 305, when the humidity detection rod 302c detects liquid, the lower end of the absorbent cotton 302d can be automatically sealed, avoiding the phenomenon that water droplets on the absorbent cotton 302d drip into the detection area 102 when the sealing plate is moved, and ensuring the cleanliness of the detection area 102.

[0048] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. A portable ultra - trace digital PCR detector, characterized in that: including a detection component (100), including a handheld PCR detector (101), a detection area (102) arranged on the handheld PCR detector (101), four storage slots (103) arranged in the detection area (102), and a thermal cover (104) arranged on the detection area (102); a leak-proof component (200), including a sealing component (201) arranged on the thermal cover (104), a replacement component (202) arranged on the sealing component (201), a guiding component (203) arranged in the detection area (102), and a one-way component (204) arranged on the guiding component (203); a display component (300), including a brightening component (301) arranged on the sealing component (201), a sensing component (302) arranged on the sealing component (201), a conductive component (303) arranged on the replacement component (202); a switching component (304) arranged on the sensing component (302); and an anti-backflow component (305) arranged on the sealing component (201).

2. The portable ultra-micro digital PCR detector according to claim 1, wherein: The sealing component (201) includes a mounting plate (201a) arranged on the lower side of the thermal cover (104), and a sealing gasket (201b) is fixedly connected to the lower end surface of the mounting plate (201a).

3. The portable ultra-trace digital PCR detector according to claim 2, wherein: The replacement component (202) includes a T-shaped groove (202a) arranged in the thermal cover (104), an L-shaped plate (202b) is hermetically and slidably connected in the T-shaped groove (202a), two sockets (202c) are arranged on the L-shaped plate (202b), a connecting plate (202d) is fixedly connected to the mounting plate (201a), a plug plate (202e) matched with the socket (202c) is fixedly connected to the connecting plate (202d), limiting ports (202f) are arranged on both the plug plate (202e) and the L-shaped plate (202b), and a plug pin (202g) is arranged in the limiting port (202f).

4. The portable ultra-micro digital PCR detector according to claim 3, wherein: The guiding component (203) includes a strip-shaped opening (203a) arranged on the thermal cover (104), a guiding rod (203b) fixedly connected to the L-shaped plate (202b) is arranged in the strip-shaped opening (203a), a horizontal groove (203c) is arranged on the inner wall of the detection area (102), a vertical groove (203d) is communicated with the front end of the horizontal groove (203c), an inclined groove (203e) is communicated with the vertical groove (203d), the inclined groove (203e) is communicated with the horizontal groove (203c), and the L-shaped plate (202b) is elastically connected to the inner wall of the T-shaped groove (202a) through a first spring (203f).

5. The portable ultra-micro digital PCR detector according to claim 4, characterized in that: The one-way component (204) includes an extension groove (204a) arranged in the inclined groove (203e), a rotating shaft (204b) is rotatably connected in the extension groove (204a), a baffle (204c) is fixedly connected to the rotating shaft (204b), and a torsion spring (204d) is arranged on the rotating shaft (204b).

6. The portable ultra-micro digital PCR detector according to claim 5, wherein: The bright light component (301) includes four lamp posts (301a) fixedly connected to the mounting plate (201a). Four controllers (301b) that cooperate with the lamp posts (301a) are fixedly connected to the mounting plate (201a). The lamp posts (301a) are divided into multiple segments, and the colors of the multiple segments of the lamp posts (301a) are all different.

7. The portable ultra-trace digital PCR detector according to claim 6, wherein: The induction component (302) includes an annular groove (302a) provided on the mounting plate (201a). Multiple partition plates (302b) are fixedly connected in the annular groove (302a). The multiple partition plates (302b) divide the annular groove (302a) into multiple induction areas. Humidity detection rods (302c) fixed to the inner wall of the annular groove (302a) are provided in the multiple induction areas. A water-absorbing cotton (302d) is provided in the annular groove (302a), and the humidity detection rods (302c) are inserted into the water-absorbing cotton (302d).

8. The portable ultra-micro digital PCR detector according to claim 6 or 7, characterized in that: The conductive component (303) includes a conductive rod (303a) provided on the L-shaped plate (202b). A receiving groove (303b) that cooperates with the conductive rod (303a) is provided on the connecting plate (202d). A copper sheet (303c) that cooperates with the conductive rod (303a) is fixedly connected to the inner wall of the receiving groove (303b).

9. The portable ultra-micro digital PCR detector according to claim 8, wherein: The switching component (304) includes a notch (304a) provided on the sealing plate. A threaded post (304b) is fixedly connected to the mounting plate (201a). A threaded sleeve (304c) is threadedly connected to the threaded post (304b). A disc (304d) that cooperates with the water-absorbing cotton (302d) is fixedly connected to the threaded sleeve (304c).

10. The portable ultra-micro digital PCR detector according to claim 9, wherein: The anti-backflow component (305) includes multiple outwardly expanding grooves (305a) provided on the sealing plate. Second springs (305b) are provided in the multiple outwardly expanding grooves (305a). A splicing plate (305c) that cooperates with the disc (304d) is fixedly connected to the second springs (305b).

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