Device and method for sampling rhizosphere and non-rhizosphere soil of wetland plants

Abstract

The invention discloses a sampling device and method for rhizosphere and non-rhizosphere soil of wetland plants, and relates to the technical field of soil sampling. Comprising a downward pressing assembly and a slice type sampler, and the slice type sampler is connected to the bottom end of the downward pressing assembly; the slice type sampler comprises a flitch plate, a side plate and a bottom plate, the bottom plate is connected to one side of the side plate, and the flitch plate is detachably connected to the side, away from the bottom plate, of the side plate; the device further comprises a grid segmentation assembly, and the grid segmentation assembly is used for marking, segmenting and sampling the samples in the slice type sampler. The slice type sampler adopted by the invention can realize collection of root system and non-root system wetland soil samples with horizontal and vertical sections in the field to obtain a complete soil section, and the slice type sampler can be partially detached and is more convenient to carry; and the sampling is segmented by using the grid mark, so that rhizosphere and non-rhizosphere soil can be accurately distinguished, and a good foundation is provided for subsequent experimental analysis.

Description

Technical Field

[0001] The present invention relates to the technical field of soil sampling, and particularly relates to a sampling device and method for rhizosphere and non-rhizosphere soils of wetland plants. Background Art

[0002] As a key microdomain environment for the interaction between roots and soil, this special area exhibits characteristics significantly different from the original soil in terms of physical and chemical properties and biological activities, and has a decisive impact on plant nutrient acquisition and soil ecological processes.

[0003] The main technical bottleneck faced by current research lies in the obvious limitations of traditional sampling methods: Although the shaking root method is simple, its accuracy is insufficient, and it is difficult to achieve hierarchical analysis of the rhizosphere microdomain. Especially in the face of wetland soil with high water content and strong viscosity, it is very difficult to distinguish rhizosphere and non-rhizosphere soils by the shaking root method; The brushing method and sieving method are also suitable for separating root soils with low water content and are difficult to apply to separating wetland root soils; It is difficult to obtain the spatial distribution of rhizosphere and non-rhizosphere soils in the wild by soil coring sampling, and it is difficult to accurately separate rhizosphere and non-rhizosphere soils in wetlands; Conventional rhizobox devices and mesh bag culture devices, etc., are limited to indoor simulation experiments and cannot meet the research on sampling rhizosphere and non-rhizosphere soils in wild wetlands.

[0004] Therefore, it is necessary to provide a sampling device and method for rhizosphere and non-rhizosphere soils of wetland plants. Summary of the Invention

[0005] The main purpose of the present invention is to provide a sampling device and method for rhizosphere and non-rhizosphere soils of wetland plants to solve the problems existing in the prior art.

[0006] To solve the above technical problems, the present invention adopts the following technical solutions: A sampling device for rhizosphere and non-rhizosphere soils of wetland plants, comprising a pressing component and a slicing sampler, and the slicing sampler is connected to the bottom end of the pressing component; The slicing sampler includes a sticking plate, side plates and a bottom plate, the bottom plate is connected to one side of the side plates, and the sticking plate is detachably connected to the side of the side plates away from the bottom plate; It further includes a grid segmentation component for marking and segmenting the samples in the slicing sampler for sampling.

[0007] Furthermore, a sticking plate installation slot is provided at the bottom end of the side plates, a clamping column adapted to the sticking plate installation slot is provided at the bottom end of the sticking plate, and snap perforations are provided at the top ends of both the side plates and the sticking plate, and snap column pins are inserted into the snap perforations.

[0008] Further, the grid segmentation component includes a horizontal array of epitaxial grids, a vertical array of epitaxial grids, and a grid plate. The ends of the horizontal array of epitaxial grids and the vertical array of epitaxial grids are aligned. The horizontal array of epitaxial grids and the vertical array of epitaxial grids are used to mark the soil segmentation areas and segmentation sizes. The horizontal array of epitaxial grids includes a plurality of first rectangular grids arranged adjacent to each other in the horizontal direction in sequence. The vertical array of epitaxial grids includes a plurality of second rectangular grids arranged adjacent to each other in the vertical direction in sequence. The grid plate has a plurality of rectangular areas. The horizontal length of each rectangular area is equal to the horizontal length of the first rectangular grid, and the vertical length of each rectangular area is equal to the vertical length of the second rectangular grid. The grid plate is used for sample segmentation.

[0009] Further, the plurality of first rectangular grids arranged adjacent to each other in the horizontal direction of the horizontal array of epitaxial grids are numbered in sequence; the plurality of second rectangular grids arranged adjacent to each other in the vertical direction of the vertical array of epitaxial grids are numbered in sequence.

[0010] Further, it further includes a concave sampling spoon, and the concave sampling spoon is used for collecting partial samples.

[0011] Further, the concave sampling spoon includes a spoon handle and a concave spoon, and the spoon handle is connected to one end of the concave spoon.

[0012] Further, the pressing-down component includes a handle, a drill rod, and a foot pedal. The handle is connected to the top end of the drill rod, and the bottom end of the drill rod is sequentially connected with a foot pedal and a slicing sampler.

[0013] A sampling method for rhizosphere and non-rhizosphere soils of wetland plants, using a sampling device for rhizosphere and non-rhizosphere soils of wetland plants, includes the following steps: S1: Select sampling points: According to the research objective, select representative wetland plant soil sampling points; S2: Assemble the sampling device: Place the sticker plate of the sampling device on one side of the side plate, and use the sticker plate installation slot and the snap stud to fix it, and assemble the slicing sampler; S3: Cut the soil profile: According to the experimental design, use the slicing sampler to vertically or horizontally cut the wetland soil to obtain a complete profile; S4: Grid segmentation sampling: Take out the slicing sampler, pull out the snap stud, remove the sticker plate, according to the plant root distribution situation, vertically press the slicing sampler through the grid segmentation component, and then use the concave sampling spoon to take out the soil sample and bag it.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. Using a slicing sampler to obtain a complete soil profile, and the slicing sampler can be partially disassembled, which is more convenient to carry.

[0015] 2. The use of grid marking for sampling can accurately distinguish rhizosphere and non-rhizosphere soils, providing a good basis for subsequent experimental analysis.

[0016] 3. The device can sample completely, enabling the collection of root and non-root wetland soil samples in horizontal and vertical profiles in the field. The integrity of the profile samples is conducive to subsequent spatial experimental data analysis.

[0017] 4. The device has a simple structure, a reasonable structural design of the sampling equipment, and a simple combination and installation. It can collect root and soil samples of plants with different root distribution conditions and is applicable to the collection of wetland environmental soil samples with high water content in the field. Description of the Drawings

[0018] Figure 1 It is a schematic structural diagram of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0019] Figure 2 It is a schematic diagram of a slicing sampler of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0020] Figure 3 It is a schematic diagram of a side plate of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0021] Figure 4 It is a schematic diagram of the connection between a patch board and a side plate of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0022] Figure 5 It is a schematic diagram of a grid segmentation component of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0023] Figure 6 It is a schematic diagram of a concave sampling spoon of a sampling device for rhizosphere and non-rhizosphere soils of wetland plants in Example 1.

[0024] Among them, 1 - handle; 2 - drill pipe; 3 - foot pedal; 4 - slicing sampler; 41 - patch board; 42 - side plate; 43 - bottom plate; 5 - plant; 6 - grid segmentation component; 61 - horizontal array extension grid; 62 - vertical array extension grid; 63 - grid board; 7 - patch board installation slot; 8 - buckle perforation; 9 - buckle pin; 10 - spoon handle; 11 - concave spoon. Detailed Embodiments

[0025] The technical solutions of the present invention will be further described below with reference to the drawings and embodiments.

[0026] Example 1

[0027] Combined with Figures 1-6, the present invention provides a sampling device for rhizosphere and non-rhizosphere soils of wetland plants, including a pressing-down component and a slicing sampler 4, and the slicing sampler 4 is connected to the bottom end of the pressing-down component; the slicing sampler 4 includes a sticking plate 41, a side plate 42 and a bottom plate 43, the bottom plate 43 is connected to one side of the side plate 42, and the sticking plate 41 is detachably connected to the side of the side plate 42 away from the bottom plate 43; further includes a grid segmentation component 6, and the grid segmentation component 6 is used to mark and segment the samples in the slicing sampler 4 for sampling, so as to accurately distinguish rhizosphere and non-rhizosphere soils.

[0028] A sticking plate installation slot 7 is arranged at the bottom end of the side plate 42, a clamping column adapted to the sticking plate installation slot 7 is arranged at the bottom end of the sticking plate 41, and buckle perforations 8 are arranged at the top ends of both the side plate 42 and the sticking plate 41. A buckle pin 9 is inserted into the buckle perforation 8. By clamping the clamping column in the sticking plate installation slot 7 and then inserting the buckle pin 9 into the buckle perforation 8, the top ends of the side plate 42 and the sticking plate 41 can be relatively fixed, and the fixing method is simple.

[0029] The grid segmentation component 6 includes a horizontally arrayed extension grid 61, a vertically arrayed extension grid 62 and a grid plate 63. The ends of the horizontally arrayed extension grid 61 and the vertically arrayed extension grid 62 are aligned. The horizontally arrayed extension grid 61 and the vertically arrayed extension grid 62 are used to mark the soil segmentation area and segmentation size; the horizontally arrayed extension grid 61 includes a plurality of first rectangular grids arranged adjacent to each other in the horizontal direction in sequence, and the vertically arrayed extension grid 62 includes a plurality of second rectangular grids arranged adjacent to each other in the vertical direction in sequence; in specific use, the horizontal width of the first rectangular grid and the vertical length of the second rectangular grid can be set as required, such as 1 cm, 2 cm, 3 cm, etc., and segmentation areas such as 1 cm * 1 cm, 2 cm * 2 cm, 3 cm * 3 cm, etc. can be formed. Different sizes of grid segmentation can effectively distinguish rhizosphere and non-rhizosphere soils according to the actual root distribution, facilitate partial sampling of rhizosphere and non-rhizosphere soils of different areas, and can reflect the spatial distribution of roots, which is beneficial to subsequent spatial experimental data analysis.

[0030] As an optimization, the plurality of first rectangular grids arranged adjacent to each other in the horizontal direction of the horizontally arrayed extension grid 61 are sequentially numbered as 1, 2, 3, 4, 5,..., n; the plurality of second rectangular grids arranged adjacent to each other in the vertical direction of the vertically arrayed extension grid 62 are sequentially numbered as 1, 2, 3, 4, 5,..., n, which is convenient for recording and numbering the samples collected in the corresponding rectangular areas.

[0031] The grid plate 63 has a plurality of rectangular regions, the lateral length of each rectangular region is equal to the lateral length of the first rectangular grid, and the vertical length of each rectangular region is equal to the vertical length of the second rectangular grid. The plurality of rectangular regions of the grid plate 63 are used for dividing the sample.

[0032] It further includes a concave sampling spoon, which is used to collect part of the sample, that is, the samples in different rectangular regions in the grid plate 63 can be collected and taken out; the concave sampling spoon includes a spoon handle 10 and a concave spoon 11, and the spoon handle 10 is connected to one end of the concave spoon 11. As an optimization, the spoon handle 10 and the concave spoon 11 can be connected by a threaded connection, which is convenient for disassembly and installation.

[0033] The pressing-down assembly includes a handle 1, a drill rod 2 and a foot pedal 3. The handle 1 is connected to the top end of the drill rod 2, and the bottom end of the drill rod 2 is sequentially connected with a foot pedal 3 and a slicing sampler 4. The slicing sampler 4 can be pressed down by the cooperation of the handle 1 and the foot pedal 3.

[0034] The present invention adopts a slicing sampler, which can realize the collection of root and non-root wetland soil samples in horizontal and vertical profiles in the wild. The profile samples are complete, which is beneficial to subsequent spatial experimental data analysis. And the slicing sampler can be partially disassembled, which is more convenient to carry.

[0035] Embodiment 2

[0036] A sampling method for rhizosphere and non-rhizosphere soil of wetland plants, using a sampling device for rhizosphere and non-rhizosphere soil of wetland plants, includes the following steps: S1: Select sampling points: According to the research objective, select representative wetland plant 5 soil sampling points; S2: Assemble the sampling device: Place the sticker of the sampling device on one side of the side plate, snap the clamping post of the sticker into the sticker installation slot on the side plate, then align the buckle perforations of the sticker and the side plate and insert the buckle pin, and use the sticker installation slot and the buckle pin for fixation to assemble the slicing sampler; S3: Cut the soil profile: According to the experimental design, use the slicing sampler to vertically or horizontally cut the wetland soil. By stepping on the foot pedal to exert force, make the slicing sampler completely enter the wetland and step on the soil surface to ensure that a complete soil profile is collected in the slicing sampler; S4: Grid division sampling: Take out the slicing sampler, pull out the buckle pin, remove the sticker, according to the plant root distribution, vertically press the grid division assembly with an appropriate spacing specification into the slicing sampler, take a photo to obtain the rhizosphere and non-rhizosphere soil distribution, and then use a matching concave sampling spoon to take out the soil samples and bag them, and number the soil samples according to the extension label of the grid division board.

[0037] As described above, this is only a preferred embodiment of the present invention and does not impose any limitation on the technical scope of the present invention. Therefore, any minor modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A sampling device for wetland plant rhizosphere and non-rhizosphere soil, characterized in that It includes a pressing-down component and a slicing sampler, and the slicing sampler is connected to the bottom end of the pressing-down component; The slicing sampler includes a sticking plate, a side plate and a bottom plate. The bottom plate is connected to one side of the side plate, and the sticking plate is detachably connected to the side of the side plate away from the bottom plate; It further includes a grid segmentation component, which is used for marking, segmenting and sampling the samples in the slicing sampler.

2. The sampling device for wetland plant rhizosphere and non-rhizosphere soil according to claim 1, characterized in that, A sticking plate installation slot is provided at the bottom end of the side plate, a clamping column adapted to the sticking plate installation slot is provided at the bottom end of the sticking plate, and buckle perforations are provided at the top ends of the side plate and the sticking plate, and buckle pins are inserted into the buckle perforations.

3. The sampling device for rhizosphere and non-rhizosphere soils of wetland plants according to claim 1, characterized in that, The grid segmentation component includes a horizontally arrayed extension grid, a vertically arrayed extension grid and a grid plate. The ends of the horizontally arrayed extension grid and the vertically arrayed extension grid are aligned. The horizontally arrayed extension grid and the vertically arrayed extension grid are used for marking the soil segmentation area and the segmentation size. The horizontally arrayed extension grid includes a plurality of first rectangular grids arranged adjacent to each other in the horizontal direction in sequence. The vertically arrayed extension grid includes a plurality of second rectangular grids arranged adjacent to each other in the vertical direction in sequence. The grid plate has a plurality of rectangular areas. The horizontal length of each rectangular area is equal to the horizontal length of the first rectangular grid, and the vertical length of each rectangular area is equal to the vertical length of the second rectangular grid. The grid plate is used for segmenting and sampling.

4. The sampling device for rhizosphere and non-rhizosphere soils of wetland plants according to claim 3, characterized in that, The plurality of first rectangular grids arranged adjacent to each other in the horizontal direction of the horizontally arrayed extension grid are numbered in sequence; the plurality of second rectangular grids arranged adjacent to each other in the vertical direction of the vertically arrayed extension grid are numbered in sequence.

5. The sampling device for wetland plant rhizosphere and non-rhizosphere soil according to claim 1, characterized in that It further includes a concave sampling spoon, which is used for collecting part of the samples.

6. The sampling device for rhizosphere and non-rhizosphere soils of wetland plants according to claim 5, characterized in that, The concave sampling spoon includes a spoon handle and a concave spoon, and the spoon handle is connected to one end of the concave spoon.

7. The sampling device for rhizosphere and non-rhizosphere soils of wetland plants according to claim 1, wherein, The pressing-down component includes a handle, a drill rod and a foot pedal. The handle is connected to the top end of the drill rod, and the bottom end of the drill rod is sequentially connected with the foot pedal and the slicing sampler.

8. A sampling method for rhizosphere and non-rhizosphere soils of wetland plants, using the sampling device for rhizosphere and non-rhizosphere soils of wetland plants described in any one of claims 1-7, characterized in that, It includes the following steps: S1: Select the sampling point: According to the research objective, select a representative wetland plant soil sampling point; S2: Combine the sampling device: Place the sticking plate of the sampling device on one side of the side plate, and use the sticking plate installation slot and the buckle pins for fixation to combine the slicing sampler; S3: Cut the soil profile: According to the experimental design, use the slicing sampler to vertically or horizontally cut the wetland soil to obtain a complete profile; S4: Grid segmentation sampling: Take out the slicing sampler, pull out the buckle pins, remove the sticking plate, press the grid segmentation component vertically into the slicing sampler according to the plant root distribution, and then use the concave sampling spoon to take out the soil samples and bag them.

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