Insecticidal composition for preventing and treating aphids

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to an insecticidal composition for preventing and treating aphids. The invention discloses an insecticidal composition for preventing and treating aphids. The insecticidal composition is prepared by compounding sophocarpidine and chlorogenic acid. The matrine and the chlorogenic acid in the insecticidal composition have different insecticidal mechanisms, and can act on pests from different acting sites during compound application, so that the resistance risk is reduced, and the generation of pest resistance to drugs is delayed. Besides, when sophocarpidine and chlorogenic acid in the insecticidal composition are compounded, the cotoxicity coefficient on aphids in a certain mass ratio range is higher than 120, the insecticidal composition has a remarkable synergistic effect, the control effect on the aphids can be improved, the application dosage of pesticides is reduced, pesticide residues and the control cost are reduced, and support is provided for developing novel insecticidal agents.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to an insecticidal composition for controlling aphids. Background Art

[0002] Aphids are important agricultural pests. At present, there are more than 4,700 recorded aphid species in the world, and more than 1,000 known species are recorded in China. There are about 100 species that can cause economic losses to agriculture. Among them, the green peach aphid and the cotton aphid are among the top ten pests in the world. Aphids have a short life cycle, a fast reproduction rate, and a wide host range. They not only directly damage agricultural and forestry crops, causing huge economic losses to agricultural and forestry production, but also indirectly damage agricultural and forestry crops by transmitting virus diseases. Nearly 50% of the currently known 600 plant viruses can use aphids as vectors. Only the green peach aphid can transmit more than 100 plant viruses, posing a huge threat to agricultural and forestry production.

[0003] At present, chemical control is still one of the main means for controlling aphids in production. With the extensive use of insecticidal agents, especially the unreasonable use of a single chemical agent such as long-term repeated and excessive application, aphids have gradually developed resistance to existing chemical agents, resulting in a gradual decrease in the efficacy of the agents. Although increasing the application dose of the agent can improve the control effect to a certain extent in the short term, it will further exacerbate the generation of drug resistance, and at the same time increase the control cost and pesticide residues. Therefore, the research and development of new compound pesticides that are safe, efficient, have different action mechanisms and have a synergistic effect not only helps to expand the insecticidal spectrum and delay the generation of pest resistance, but also can save costs and reduce the pollution of agricultural products and the environment.

[0004] Pesticide compounding is a common means for developing new pesticide products and controlling resistant pests and diseases at present. The compounded agent will show antagonistic, additive or synergistic effects. Compared with a single agent, the compounded agent showing a synergistic effect can effectively improve the actual control effect on pests and diseases, delay the generation of pest and disease resistance, and can also reduce the control cost and pesticide residues.

[0005] Matrine, a natural alkaloid compound extracted and isolated from leguminous plants such as Sophora flavescens, Sophora alopecuroides, and Sophora subprostrata, is commonly used to control pests such as aphids, thrips, diamondback moths, Plutella xylostella, and spider mites. Its insecticidal activity is mainly contact toxicity and supplemented by stomach toxicity. Some research reports have shown that matrine may act on the neuro-muscular system of insects, and the glutamate-gated chloride channel is one of its action targets. Chlorogenic acid, a natural phenolic acid compound widely distributed in various plants, can inhibit the growth, development and reproduction of pests such as Protaetia brevitarsis, Lymantria dispar, and Tenebrio molitor, and has broad development prospects in the field of pesticides.

[0006] There is no report on the combined application of matrine and chlorogenic acid in aphid control. Through research, the inventors found that after the combination of matrine and chlorogenic acid, it showed good synergistic effect on aphids, based on which it can provide support for the development of new insecticidal agents. Summary of the Invention

[0007] The purpose of the present invention is to provide an insecticidal composition for controlling aphids to solve the problems existing in the long-term application of a single agent.

[0008] To achieve the above purpose, the present invention provides the following technical solutions:

[0009] The first purpose of the present invention is to provide an insecticidal composition for controlling aphids, which is composed of the combination of matrine and chlorogenic acid.

[0010] Preferably, the mass ratio of matrine to chlorogenic acid is 1-0.8:1-4.

[0011] The second purpose of the present invention is to provide an insecticide, including the above-mentioned insecticidal composition for controlling aphids and auxiliary components allowed to be used in pesticides.

[0012] Preferably, the mass of the insecticidal composition for controlling aphids accounts for 0.5-80% of the total mass of the insecticide. The auxiliary components allowed to be used in the pesticide are selected from one or more of solvents, cosolvents, emulsifiers, wetting agents, dispersants, thickeners, antifreezing agents, preservatives, defoaming agents, disintegrants and carriers.

[0013] The third purpose of the present invention is to provide the application of the above-mentioned insecticidal composition for controlling aphids or the above-mentioned insecticide in controlling aphids.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The insecticidal mechanisms of matrine and chlorogenic acid in the insecticidal composition of the present invention are different. When applied in combination, they can act on pests from different action sites, which is beneficial to reducing the resistance risk and delaying the generation of pest resistance.

[0016] (2) When matrine and chlorogenic acid are combined in the insecticidal composition of the present invention, the co-toxicity coefficient to aphids is higher than 120 within a certain mass ratio range, showing a significant synergistic effect. It can improve the control effect on aphids, is beneficial to reducing the application dose of pesticides, reducing pesticide residues and control costs, and can provide support for the development of new insecticidal agents. Detailed Embodiments

[0017] According to the following embodiments, the present invention can be better understood. The content described in the embodiments is only used to illustrate the present invention and should not and will not limit the present invention described in detail in the claims.

[0018] Example 1: 45% Matrine·Chlorogenic Acid Wettable Powder

[0019] The weight percentage (%) of the insecticidal active ingredients is: matrine 25.0, chlorogenic acid 20.0;

[0020] The weight percentage (%) of each component of the auxiliary agent is: wetting agent: sodium lauryl sulfate 20.0; dispersant: M9 18.0; filler: kaolin 17.0.

[0021] The matrine and chlorogenic acid technical drugs are pulverized by air flow according to the ratio and then added into a mixer. The dispersant and filler are respectively added into the mixer according to the ratio, and stirred evenly to obtain the product.

[0022] Example 2: 1.5% Matrine·Chlorogenic Acid Emulsion in Water

[0023] The weight percentage (%) of the insecticidal active ingredients is: matrine 0.5, chlorogenic acid 1.0;

[0024] The weight percentage (%) of each component of the auxiliary agent is: emulsifier: naphthalene sulfonate formaldehyde condensate (NNO) 8.0; solvent: S-150 10.0; thickener: xanthan gum 0.2, magnesium aluminum silicate 1.0; defoamer: EO-PO polymer 0.3; preservative: sodium benzoate 0.8; antifreeze: ethylene glycol 6.0; the balance is deionized water.

[0025] The matrine and chlorogenic acid technical drugs are dissolved in the solvent according to the ratio. The emulsifier and cosolvent are added into the matrine and chlorogenic acid solution, stirred evenly, and the above mixture is slowly added into water and stirred evenly to obtain the product.

[0026] Example 3: 5.0% Matrine·Chlorogenic Acid Microemulsion

[0027] The weight percentage (%) of the insecticidal active ingredients is: matrine 1.0, chlorogenic acid 4.0;

[0028] The weight percentage (%) of each component of the auxiliary agent is: emulsifier: polyoxyethylene alkylamine 20.0; cosolvent: ethyl acetate 0.05; defoamer: high-efficiency polysiloxane 0.5; antifreeze: glycerol 0.5; solvent: Solvesso100 20.0; the balance is deionized water.

[0029] The matrine and chlorogenic acid are dissolved in the solvent according to the ratio. The emulsifier is added into the matrine and chlorogenic acid solution, stirred evenly, deionized water and the remaining auxiliary agents are added, and the mixture is put into a homogenizer for strong mixing and homogenization to obtain the product.

[0030] The following combines test examples to illustrate its effects.

[0031] Test Example: Indoor Toxicity Determination Test

[0032] 1. Test Insects: Myzus persicae collected from peach trees were reared in the laboratory, and those of similar size were selected as the test subjects.

[0033] 2. Test Chemicals: 98% Matrine (Shanghai Macklin Biochemical Co., Ltd.), 98% Chlorogenic Acid (Shanghai Macklin Biochemical Co., Ltd.).

[0034] The test chemicals were dissolved in ethanol to prepare single-agent mother liquors, and then diluted with 0.1% Tween-80 aqueous solution. Multiple groups of ratios were set, and 7 gradient mass concentrations were set for each single agent and each mixture ratio of the mixture according to the geometric progression method for standby.

[0035] 3. Test Method: Refer to "NY / T 1154.14-2008 Guidelines for Pesticide Bioassay in the Laboratory - Part 14: Leaf-dipping Method for Insecticides".

[0036] Fresh peach leaves of equal size were immersed in the prepared test solutions of different concentration gradients, taken out and dried after 10 s, placed in a petri dish containing moist filter paper, inoculated with test insects, sealed with gauze, and the test insects were continuously reared in the laboratory. Each treatment was set with 4 replicates, 30 test insects in each replicate, and a solution treatment containing only ethanol and 0.1% Tween-80 was set as the blank control. The death situation of the test insects was investigated 48 h after treatment, the total number of insects and the number of dead insects were recorded, and the corrected mortality of each treatment was calculated.

[0037]

[0038] Where: P—the mortality rate, in percentage (%); K—the number of dead insects, in number; N—the total number of insects in the treatment, in number.

[0039]

[0040] Where: P1—the corrected mortality rate, in percentage (%); P t —the treatment mortality rate, in percentage (%); P0—the blank control mortality rate, in percentage (%).

[0041] If the control mortality rate < 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be carried out; if the control mortality rate > 20%, the test needs to be redone.

[0042] 4. Data Analysis: Use EXCEL software to conduct regression analysis on the logarithm of the chemical concentration of each treatment and the probit value of the corrected mortality rate of the test insects, calculate the LC 50 of each treatment chemical, and calculate the co-toxicity coefficient (CTC) of the mixture according to the Sun Yunpei method.

[0043]

[0044] Where: ATI—the measured toxicity index of the mixture; S—the LC 50 , in mg / L; M—the LC[[ID=X]] 50 , in mg / L.

[0045] TTI = TI A ×P A + TI B ×P B

[0046] Where: TTI—the theoretical toxicity index of the mixture; TI A —the toxicity index of agent A; P A —the percentage content of agent A in the mixture, in percentage (%); TI B —the toxicity index of agent B; P B —the percentage content of agent B in the mixture, in percentage (%).

[0047]

[0048] Where: CTC—coefficient of co-toxicity; ATI—the measured toxicity index of the mixture; TTI—the theoretical toxicity index of the mixture.

[0049] 5. Efficacy evaluation: Evaluate the efficacy of the mixture according to the calculated coefficient of co-toxicity (CTC). When CTC ≤ 80, it indicates antagonistic effect; when 80 < CTC < 120, it indicates additive effect; when CTC ≥ 120, it indicates synergistic effect. The results are shown in Table 1.

[0050] Table 1 Indoor toxicity determination results of the mixture of matrine and chlorogenic acid against aphids

[0051]

[0052] As can be seen from Table 1, in the mass ratio range of 1 - 0.8:1 - 4, the coefficient of co-toxicity of the mixture of matrine and chlorogenic acid against aphids is greater than 120, showing a synergistic effect. Especially when the mass ratio is 1:0.8, the coefficient of co-toxicity reaches 311.90, and the synergistic effect is the most significant.

[0053] In summary, when matrine and chlorogenic acid are mixed, it shows a synergistic effect within a certain mass ratio range, which can improve the control effect against aphids. Based on this, the application dose of the pesticide can be reduced, and problems such as pesticide residues and environmental pollution caused by large-dose application can be avoided to a certain extent.

[0054] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many modifications and variations are possible in light of the above teaching. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can implement and utilize the various different exemplary embodiments of the present invention, as well as various different selections and modifications. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. An insecticidal composition for controlling aphids, characterized in that, The insecticidal composition for controlling aphids is prepared by compounding matrine and chlorogenic acid, and the mass ratio of matrine to chlorogenic acid is 1-0.8:1-4.

2. An insecticide, characterized in that, It includes the insecticidal composition for controlling aphids described in claim 1 and auxiliary components allowed to be used in pesticides.

3. The pesticide according to claim 2, characterized in that, The mass of the insecticidal composition for controlling aphids accounts for 0.5-80% of the total mass of the insecticide.

4. The pesticide according to claim 2, characterized in that, The auxiliary components allowed to be used in the pesticide are selected from one or more of solvents, cosolvents, emulsifiers, wetting agents, dispersants, thickeners, antifreezes, preservatives, defoamers, disintegrants and carriers.

5. Application of the insecticidal composition for controlling aphids described in claim 1 or the insecticide described in claims 2-4 in controlling aphids.

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