Acidic high-content collagen beverage and preparation method thereof

Abstract

The invention discloses an acidic high-content collagen beverage, which is prepared from the following raw materials in percentage by mass: 30 to 34 percent of collagen peptide, 8 to 12 percent of collagen tripeptide, 0.05 to 0.3 percent of elastin peptide, 0.01 to 0.05 percent of hyaluronic acid, 0.1 to 0.3 percent of grape seed extract, 0.05 to 0.1 percent of xylooligosaccharide, 0.01 to 0.03 percent of sweetening agent, 0.8 to 1.5 percent of acidulant and the balance of water. The pH value of the acidic high-content collagen beverage is less than or equal to 4.6. The acidic high-content collagen beverage disclosed by the invention has the characteristics of excellent taste, high collagen content, acidity, no bad flavor and stable quality.

Description

Technical Field

[0001] The present invention relates to the field of food technology, and particularly to an acidic high-content collagen drink and a preparation method thereof. Background Art

[0002] In the field of beauty and anti-aging, collagen peptides have attracted much attention due to their functions of maintaining the morphological structure of the skin and repairing damaged tissues. 70% of the components in human skin are composed of collagen. As an important part of the whole body's proteins (accounting for 1 / 3), it plays a key role in delaying skin aging and reducing wrinkles.

[0003] Currently, most existing products are low-content collagen peptide drinks (usually the collagen peptide content is less than 30%), and the anti-wrinkle and anti-aging effects are limited. If the protein content in the drink is high, microbial proliferation is likely to occur during the shelf life, affecting the quality and safety of the product. Traditional collagen drinks usually control the pH value in the slightly acidic range (pH≥4.6) to avoid protein denaturation or microbial contamination, resulting in a narrow variety of acidulants that can be selected and a single taste (such as relying only on citric acid to adjust the acidity). At the same time, the neutral environment is prone to microbial proliferation problems of high-content proteins during the shelf life, and antioxidants or preservatives are needed to maintain stability, affecting the safety and flavor of the product. Summary of the Invention

[0004] Therefore, based on the above defects of the prior art, the present invention provides an acidic high-content collagen drink and a preparation method thereof.

[0005] To solve the above technical problems, the present invention provides the following technical solutions:

[0006] The first aspect of the present invention provides an acidic high-content collagen drink, which is composed of the following raw materials by mass percentage: 30-34% of collagen peptide, 8-12% of collagen tripeptide, 0.05-0.3% of elastin peptide, 0.01-0.05% of hyaluronic acid, 0.1-0.3% of grape seed extract, 0.05-0.1% of xylo-oligosaccharide, 0.01-0.03% of sweetener, 0.8-1.5% of acidulant, and the balance of water; the pH value of the acidic high-content collagen drink is ≤4.6.

[0007] In the acidic high-content collagen drink of the present invention, 0.01-0.05% of hyaluronic acid is added, which forms a synergistic effect with collagen to enhance the skin's ability to retain moisture; secondly, 0.1-0.3% of grape seed extract is added, which is rich in proanthocyanidins, improving the antioxidant capacity of the product and being beneficial to delaying skin aging. In addition, 0.05-0.1% of xylo-oligosaccharide is added, which can selectively promote the proliferation activity of intestinal Bifidobacterium, has good prebiotic activity, is beneficial to regulating the intestinal microecology, and indirectly promotes the absorption of collagen.

[0008] In the acidic high-content collagen drink of the present invention, collagen peptide and elastin peptide are added simultaneously to form a "dual-protein anti-aging system", which can strengthen the repair ability of skin elastic fibers. In addition, a relatively high content of collagen tripeptide (8-12%) is added. Because its molecular weight is smaller (<1000Da), the absorption efficiency is higher, and the anti-wrinkle effect can be significantly enhanced.

[0009] Further, in the acidic high-content collagen drink, the mass ratio of elastin peptide to collagen peptide is 1:(100-180).

[0010] Further, in the acidic high-content collagen drink, the collagen peptide is fish collagen peptide derived from deep-sea cod skin. Deep-sea cod skin has the characteristics of low fat, low heavy metal residue, and high absorption rate, and has better safety.

[0011] Further, the collagen peptide is obtained by enzymatic hydrolysis with a composite protease at a temperature of 40-50°C and a pH of 6.5-7.5 for 12-24 hours; the composite protease is composed of trypsin and neutral protease, and their mass ratio is 1:1.

[0012] In the present invention, through bioenzyme-directed cleavage, the molecular weight of the collagen peptide can be concentrated between 1000 and 3000Da, improving the bioavailability.

[0013] Further, the molecular weight of the collagen peptide is between 1000 and 3000Da.

[0014] Further, the pH value of the acidic high-content collagen drink is 4.2-4.5.

[0015] Further, both the collagen peptide and the elastin peptide are microcapsules with a particle size of 10-50μm formed by β-cyclodextrin embedding treatment.

[0016] In the present invention, through β-cyclodextrin embedding treatment, the peptide components can be protected from being damaged by the acidic environment, and their stability below a pH value of 4.6 is increased by 40%; at the same time, it can control the slow release and extend the action time.

[0017] Furthermore, the sweetener includes one or more of brown sugar, sucralose, trichloroglucose, erythritol, stevioside, aspartame, neotame.

[0018] Furthermore, the sour agent includes one or more of citric acid, lactic acid, malic acid, tartaric acid, fumaric acid, sodium citrate.

[0019] Furthermore, the sweetener consists of the following components: 0.005 - 0.01% of sucralose, 0.005 - 0.02% of erythritol; the sour agent consists of the following components: 0.5 - 0.8% of citric acid, 0.2 - 0.5% of malic acid, 0.1 - 0.2% of lactic acid.

[0020] In the present invention, a novel combination of sour agents - the compounding system of "0.5 - 0.8% of citric acid, 0.2 - 0.5% of malic acid, 0.1 - 0.2% of lactic acid" is obtained through screening, making the taste present the characteristics of "sour first and sweet later, with rich layers". At the same time, the sour taste is balanced by pH regulation (4.2 - 4.5), and the astringency problem in a high - acid environment is solved through the synergistic effect of the sweetener combination of "0.005 - 0.01% of sucralose, 0.005 - 0.02% of erythritol".

[0021] In the second aspect of the present invention, a preparation method of the acidic high - content collagen drink is provided, including the following steps:

[0022] S1. Dissolve the sour agent in 50% water and adjust the pH to 3.5 - 4.0;

[0023] S2. Add collagen peptide and collagen tripeptide, and stir at a high speed of 200 - 300 rpm for 10 - 30 minutes to fully swell the collagen peptide and collagen tripeptide;

[0024] S3. Add elastin peptide, sweetener, hyaluronic acid, grape seed extract and xylo - oligosaccharide in sequence, and stir at a low speed of 50 - 100 rpm for 5 - 15 minutes;

[0025] S4. Make up the water to the total amount, homogenize at a pressure of 100 - 150 MPa for 1 - 3 times, and adjust the pH to below 4.6, then the acidic high - content collagen drink is obtained.

[0026] In the present invention, the above - mentioned gradient mixing process can avoid protein aggregation, improve the clarity of the solution, and make the light transmittance ≥ 95%.

[0027] Furthermore, in steps S2 and S3, the collagen peptide and elastin peptide have been subjected to an embedding treatment, and the method of the embedding treatment is:

[0028] Prepare an aqueous solution of β-cyclodextrin with a concentration of 0.1 - 0.3 wt%, add collagen peptide or elastin peptide, and at the same time add an emulsifier, mix under a high-speed disperser, and then perform high-pressure homogenization treatment 1 - 3 times to make the particle size of the suspension ≤ 5 μm; spray-dry the suspension, and sieve the obtained powder to collect microcapsules with a particle size of 10 - 50 μm.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0030] 1. The acidic high-content collagen drink provided by the present invention is added with hyaluronic acid, grape seed extract and xylooligosaccharide. Among them, hyaluronic acid can form a synergistic effect with collagen to enhance the skin's moisturizing and water-locking ability; the grape seed extract is rich in proanthocyanidins, which improves the antioxidant ability of the product and is beneficial to delaying skin aging; xylooligosaccharide can selectively promote the proliferation activity of intestinal Bifidobacterium, has good prebiotic activity, is beneficial to regulating the intestinal microecology, and indirectly promotes the absorption of collagen.

[0031] 2. For the acidic high-content collagen drink provided by the present invention, both collagen peptide and elastin peptide are encapsulated by β-cyclodextrin, which can protect the peptide components from being damaged by the acidic environment, and their stability below pH 4.6 is increased by 40%. Therefore, a high content of collagen can be added to acidic drinks, providing sufficient collagen raw materials for skin repair, thus being beneficial to reducing skin wrinkles and enhancing the anti-aging effect; at the same time, β-cyclodextrin encapsulation can achieve the slow release of collagen peptide and elastin peptide, prolonging the action time.

[0032] 3. The acidic high-content collagen drink provided by the present invention can expand the pH value range to acidic, thus increasing the raw material range of collagen drinks. Acidulants can also be applied to collagen drinks, making the collagen drink have an excellent taste. The drink has a high collagen content, an acidic pH, no bad flavor, and stable quality. Detailed implementation mode

[0033] The following further illustrates the present invention with specific embodiments, so that those skilled in the art can better understand the present invention and be able to implement it, but the specific embodiments cited do not limit the present invention.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. The terms used in the description of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0035] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are all conventional methods. The materials, reagents, etc. used can be obtained from commercial sources unless otherwise specified.

[0036] Example 1

[0037] This example provides an acidic high-content collagen drink, which is composed of the following raw materials by mass percentage: 32% of collagen peptide, 10% of collagen tripeptide, 0.2% of elastin peptide, 0.02% of hyaluronic acid, 0.2% of grape seed extract, 0.1% of xylo-oligosaccharide, 0.005% of sucralose, 0.02% of erythritol, 0.6% of citric acid, 0.3% of malic acid, 0.1% of lactic acid and the balance of water; the pH of the acidic high-content collagen drink is 4.5.

[0038] The preparation method of the acidic high-content collagen drink in this example includes the following steps:

[0039] S1. Prepare an aqueous solution of β-cyclodextrin with a concentration of 0.2 wt%, add collagen peptide or elastin peptide, and at the same time add 1 wt% of emulsifier Tween-80 based on the mass of β-cyclodextrin, mix under a high-speed disperser, and then perform high-pressure homogenization treatment twice to make the particle size of the suspension ≤ 5 μm; spray-dry the suspension, and screen the obtained powder to collect microcapsules with a particle size of 10 - 50 μm to obtain collagen peptide and elastin peptide embedded with β-cyclodextrin.

[0040] S2. Dissolve citric acid, malic acid and lactic acid in 50% water and adjust the pH to 3.8.

[0041] S3. Add collagen peptide embedded with β-cyclodextrin and collagen tripeptide, and stir at a high speed of 300 rpm for 10 minutes to fully swell the collagen peptide and collagen tripeptide.

[0042] S3. Sequentially add elastin peptide embedded with β-cyclodextrin, sucralose, erythritol, hyaluronic acid, grape seed extract and xylo-oligosaccharide, and stir at a low speed of 50 rpm for 15 minutes.

[0043] S4. Make up the water to the total amount, homogenize twice at a pressure of 100 MPa, and adjust the pH to 4.5 to obtain the acidic high-content collagen drink.

[0044] Example 2

[0045] This embodiment provides an acidic high-content collagen drink, which is composed of the following raw materials by mass percentage: 30% of collagen peptide, 8% of collagen tripeptide, 0.3% of elastin peptide, 0.03% of hyaluronic acid, 0.1% of grape seed extract, 0.05% of xylo-oligosaccharide, 0.005% of sucralose, 0.02% of erythritol, 0.6% of citric acid, 0.3% of malic acid, 0.1% of lactic acid and the balance of water; the pH of the acidic high-content collagen drink is 4.5.

[0046] The preparation method of the acidic high-content collagen drink of this embodiment includes the following steps:

[0047] S1. Prepare an aqueous solution of β-cyclodextrin with a concentration of 0.1 wt%, add collagen peptide or elastin peptide, and at the same time add 1 wt% of emulsifier Tween-80 based on the mass of β-cyclodextrin, mix under a high-speed disperser, and then perform high-pressure homogenization treatment twice to make the particle size of the suspension ≤ 5 μm; spray-dry the suspension, screen the obtained powder, and collect microcapsules with a particle size of 10 - 50 μm to obtain collagen peptide and elastin peptide embedded with β-cyclodextrin;

[0048] S2. Dissolve citric acid, malic acid and lactic acid in 50% of water and adjust the pH to 4.0;

[0049] S3. Add collagen peptide embedded with β-cyclodextrin and collagen tripeptide, and stir at a high speed of 300 rpm for 10 minutes to fully swell the collagen peptide and collagen tripeptide;

[0050] S3. Sequentially add elastin peptide embedded with β-cyclodextrin, sucralose, erythritol, hyaluronic acid, grape seed extract and xylo-oligosaccharide, and stir at a low speed of 50 rpm for 15 minutes;

[0051] S4. Make up the water to the total amount, homogenize twice at a pressure of 100 MPa, and adjust the pH to 4.5 to obtain the acidic high-content collagen drink.

[0052] Example 3

[0053] This embodiment provides an acidic high-content collagen drink, which is composed of the following raw materials by mass percentage: 34% of collagen peptide, 12% of collagen tripeptide, 0.2% of elastin peptide, 0.05% of hyaluronic acid, 0.3% of grape seed extract, 0.1% of xylo-oligosaccharide, 0.005% of sucralose, 0.02% of erythritol, 0.6% of citric acid, 0.3% of malic acid, 0.1% of lactic acid and the balance of water; the pH of the acidic high-content collagen drink is 4.5.

[0054] The preparation method of the acidic high-content collagen drink of this embodiment includes the following steps:

[0055] S1. Prepare an aqueous solution of β-cyclodextrin with a concentration of 0.3 wt%, add collagen peptide or elastin peptide, and simultaneously add 1 wt% of emulsifier Tween-80 based on the mass of β-cyclodextrin. Mix under a high-speed disperser, and then perform high-pressure homogenization treatment twice to make the particle size of the suspension ≤ 5 μm. Spray-dry the suspension, sieve the obtained powder, and collect microcapsules with a particle size of 10 - 50 μm to obtain collagen peptide and elastin peptide embedded with β-cyclodextrin.

[0056] S2. Dissolve citric acid, malic acid, and lactic acid in 50% water and adjust the pH to 4.0.

[0057] S3. Add collagen peptide embedded with β-cyclodextrin and collagen tripeptide, and stir at a high speed of 300 rpm for 10 minutes to fully swell the collagen peptide and collagen tripeptide.

[0058] S3. Sequentially add elastin peptide embedded with β-cyclodextrin, sucralose, erythritol, hyaluronic acid, grape seed extract, and xylo-oligosaccharide, and stir at a low speed of 50 rpm for 15 minutes.

[0059] S4. Make up the water to the total amount, homogenize twice at a pressure of 100 MPa, and adjust the pH to 4.5 to obtain an acidic high-content collagen drink.

[0060] Comparative Example 1

[0061] This comparative example provides an acidic high-content collagen drink, and the difference from Example 1 is that grape seed extract is replaced with vitamin C, and hyaluronic acid is replaced with an equal amount of elastin peptide.

[0062] Comparative Example 2

[0063] This comparative example provides an acidic high-content collagen drink, and the difference from Example 1 is that xylo-oligosaccharide is replaced with fructo-oligosaccharide.

[0064] Comparative Example 3

[0065] This comparative example provides an acidic high-content collagen drink, and the difference from Example 1 is that collagen peptide and collagen tripeptide are not treated with β-cyclodextrin embedding.

[0066] Experiment 1

[0067] This experiment was carried out in accordance with T / GDCDC 019-2021 "Test Method for Anti-Wrinkle Efficacy of Cosmetics", specifically as follows:

[0068] Seventy healthy female subjects aged between 35 and 60 were selected and divided into 7 groups. The subjects were required to have lack of elasticity in facial skin, loose skin, dull skin color, and visible wrinkles; and they did not use products with the same effects as the test product, such as repair, firming, and anti-wrinkle effects, one month before the experiment.

[0069] The subjects in each group drank the acidic high-content collagen drinks of Examples 1-3 and Comparative Examples 1-4 once a day, with a dosage of 500 mL; and drank continuously for 14 days. Facial skin pictures of the subjects were collected by Primos CR and image analysis was carried out.

[0070] Data statistics: Statistical processing was carried out using SPSS 10.0 software, and the data was expressed by , t-test was used for comparison between groups, and analysis of variance was used for comparison of means of multiple groups. When P≤0.05, it indicated a significant difference.

[0071] Table 1

[0072]

[0073]

[0074] Please refer to Table 1. After 14 days of the human efficacy evaluation test, when the subjects used the drinks of Examples 1-3, compared with before use: the wrinkle depth, wrinkle volume, and wrinkle area ratio all showed a decreasing trend, and there were significant differences in the two indicators of wrinkle volume and wrinkle area ratio (P<0.01). This shows that the acidic high-content collagen drink of the present invention has the effect of slowing down the generation of skin wrinkles.

[0075] After 14 days of the subjects using the drinks of Comparative Examples 1-3, compared with before use: although the wrinkle depth, wrinkle volume, and wrinkle area ratio also showed a decreasing trend, the decreasing amplitude was significantly smaller than that of Example 1, and there were no significant differences in the three indicators of wrinkle depth, wrinkle volume, and wrinkle area ratio (P≥0.05).

[0076] In Comparative Example 1, grape seed extract was replaced with vitamin C, and hyaluronic acid was replaced with an equal amount of elastin peptide. The proanthocyanidins rich in grape seed extract have extremely strong antioxidant properties, and its antioxidant effect is dozens of times that of vitamin C. Hyaluronic acid can synergistically enhance the skin's moisturizing and water-locking ability with collagen peptide. Therefore, Example 1 has a better anti-aging effect than Comparative Example 1.

[0077] In Comparative Example 2, xylooligosaccharide was replaced with fructooligosaccharide. Since xylooligosaccharide can selectively promote the proliferation activity of intestinal Bifidobacterium, has good prebiotic activity, is beneficial to regulating the intestinal microecology, and indirectly promotes the absorption of collagen, it is also beneficial to improving the anti-aging effect.

[0078] In Comparative Example 3, collagen peptide and collagen tripeptide were not treated by β-cyclodextrin inclusion. Under acidic conditions with pH = 4.5, these peptide components are unstable in the acidic environment and will be destroyed, resulting in a reduction in their anti-aging and wrinkle-reducing effects.

[0079] The above-described embodiments are only preferred embodiments cited to fully illustrate the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of the present invention are all within the protection scope of the present invention. The protection scope of the present invention is subject to the claims.

Claims

1. An acidic high-content collagen drink, characterized in that, It comprises the following raw materials by mass percentage: 30-34% of collagen peptide, 8-12% of collagen tripeptide, 0.05-0.3% of elastin peptide, 0.01-0.05% of hyaluronic acid, 0.1-0.3% of grape seed extract, 0.05-0.1% of xylo-oligosaccharide, 0.01-0.03% of sweetener, 0.8-1.5% of acidulant and the balance of water; the pH value of the acidic high-content collagen drink is ≤ 4.

6.

2. The acidic high-content collagen drink according to claim 1, characterized in that, In the acidic high-content collagen drink, the mass ratio of elastin peptide to collagen peptide is 1:(100-180).

3. An acidic high-content collagen drink according to claim 1, characterized in that, In the acidic high-content collagen drink, the collagen peptide is fish collagen peptide derived from deep-sea cod skin.

4. An acidic high-content collagen drink according to claim 3, characterized in that, The collagen peptide is obtained by enzymatic hydrolysis with a compound protease at 40-50 °C and pH = 6.5-7.5 for 12-24 hours; the compound protease is composed of trypsin and neutral protease, and their mass ratio is 1:

1.

5. An acidic high-content collagen drink according to claim 4, characterized in that, The molecular weight of the collagen peptide is between 1000 and 3000 Da; and / or, The pH value of the acidic high-content collagen drink is 4.2-4.

5.

6. The acid high-content collagen drink according to claim 1, characterized in that Both the collagen peptide and the elastin peptide are microcapsules with a particle size of 10-50 μm formed by β-cyclodextrin embedding treatment.

7. An acidic high-content collagen drink according to claim 1, characterized in that, The sweetener includes one or more of brown sugar, sucralose, trichloroglucose, erythritol, stevioside, aspartame, neotame; The acidulant includes one or more of citric acid, lactic acid, malic acid, tartaric acid, fumaric acid, sodium citrate.

8. An acidic high-content collagen drink according to claim 7, characterized in that, The sweetener consists of the following components: 0.005-0.01% of sucralose, 0.005-0.02% of erythritol; and / or, The acidulant consists of the following components: 0.5-0.8% of citric acid, 0.2-0.5% of malic acid, 0.1-0.2% of lactic acid.

9. The preparation method of an acidic high-content collagen drink according to claim 1, characterized in that, [[ID=1I]]It includes the following steps: S1. Dissolve the acidulant in 50% of water and adjust the pH to 3.5-4.0; S2. Add the collagen peptide and collagen tripeptide, and stir at a high speed of 200-300 rpm for 10-30 minutes to fully swell the collagen peptide and collagen tripeptide; S3. Add the elastin peptide, sweetener, hyaluronic acid, grape seed extract and xylo-oligosaccharide in sequence, and stir at a low speed of 50-100 rpm for 5-15 minutes; S4. Make up the water to the total amount, homogenize at a pressure of 100-150 MPa for 1-3 times, and adjust the pH to below 4.6 to obtain the acidic high-content collagen drink.

10. The preparation method of an acidic high-content collagen drink according to claim 9, characterized in that, In steps S2 and S3, both the collagen peptide and the elastin peptide have undergone embedding treatment, and the method of the embedding treatment is: Prepare an aqueous solution of β-cyclodextrin with a concentration of 0.1-0.3 wt%, add the collagen peptide or elastin peptide, and at the same time add an emulsifier, mix under a high-speed disperser, and then perform high-pressure homogenization treatment for 1-3 times to make the particle size of the suspension ≤ 5 μm; then, spray-dry the suspension, and screen the obtained powder to collect microcapsules with a particle size of 10-50 μm.

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