Anti-wrinkle composition, use and skin care product

Abstract

The present invention belongs to the field of skin care technology and discloses an anti-wrinkle composition, its use, and a skin care product. The anti-wrinkle composition comprises a sunflower mallow extract and an evening primrose root extract, obtained by compounding sunflower mallow and evening primrose root in a weight ratio of 3-7:3-9. It also comprises a safflower flower extract, obtained by compounding sunflower mallow, evening primrose root, and safflower in a weight ratio of 2-8:3-9:5-20. The present invention provides an anti-wrinkle extract, wherein the combination of the sunflower mallow extract and evening primrose root extract can synergistically enhance the skin anti-wrinkle efficacy of the composition.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care, and in particular to an anti-wrinkle composition, its application and a skin care product. Background Art

[0002] Anti-wrinkle skin care is not only about maintaining a youthful appearance, but also about scientifically maintaining skin health. With aging and ongoing damage from the external environment (such as UV rays and free radicals), skin collagen is gradually lost, elasticity decreases, and wrinkles form. Anti-wrinkle care stimulates collagen regeneration, delays oxidative aging, and repairs barrier function. This not only reduces existing wrinkles but also prevents new ones, thus maintaining a firm and plump skin. Furthermore, smooth, refined skin is often closely linked to personal confidence and quality of life. Therefore, anti-wrinkle is both a physiological need and an extension of psychological and social image management.

[0003] Based on this, many skin anti-wrinkle products are active on the market, such as prior art 1: Chinese patent application 202211469185.1 discloses an anti-wrinkle composition: hydrolyzed collagen, arginine / lysine polypeptide, and ethylbisiminomethylguaiacol manganese chloride. The hydrolyzed collagen can increase the production of collagen and hyaluronic acid, reduce skin sagging, and improve skin elasticity. The compound arginine / lysine polypeptide promotes collagen regeneration. The strong muscle relaxation effect has a rapid and immediate anti-wrinkle effect. Ethylbisiminomethylguaiacol manganese chloride has a good antioxidant effect, which reduces skin damage and causes a decrease in skin moisture, elasticity, and firmness, thereby achieving a firming effect on the skin.

[0004] As in prior art 2: Chinese patent application 201810802205.X discloses a cosmetic composition with skin elasticity and anti-wrinkle functions and its application. The cosmetic composition contains: 0.01% to 1.0% ceramide; 0.001% to 0.1% palmitoyl oligopeptide; 0.05% to 1.0% royal jelly; and 0.25% to 5% lecithin. The weight ratio of royal jelly (on a dry weight basis) to lecithin described in the present invention is 1:5, and the composition is in the form of a double-layer liposome aqueous solution with a particle size of 30nm to 130nm. The cosmetic composition of the present invention containing ceramide, palmitoyl oligopeptide, royal jelly, and lecithin can achieve multiple benefits, including improving skin wrinkles, increasing skin elasticity, and restoring skin function.

[0005] It can be seen from the existing technologies 1 and 2 that the compounding of multiple components can obtain cosmetic compositions with good anti-wrinkle effects, which is a good research direction; however, due to the increasingly fierce competition in the cosmetics market, consumers have an increasingly high demand for diversified skin care products. In order to enhance the market competitiveness of products, it is necessary to develop more anti-wrinkle compositions to meet the diverse needs of consumers. Summary of the Invention

[0006] One of the purposes of the present invention is to provide an anti-wrinkle composition obtained by compounding a sunflower extract and an evening primrose root extract, which has excellent skin anti-wrinkle effect and can meet the needs of consumers.

[0007] Another object of the present invention is to provide a use of an anti-wrinkle composition, wherein the anti-wrinkle composition is used to prepare a skin care product, so that the skin care product can have excellent skin anti-wrinkle effect.

[0008] At the same time, the present invention also provides a skin care product, which provides the market with more skin care options and meets the diverse needs of consumers.

[0009] To achieve the above object, the present invention provides an anti-wrinkle composition, comprising a sunflower mallow extract and an evening primrose root extract, wherein the sunflower mallow extract and the evening primrose root extract are obtained by composite extraction of sunflower mallow and evening primrose root in a weight ratio of 3-7:3-9.

[0010] The oil-soluble components in sunflower have strong antioxidant activity, which can scavenge free radicals, block ultraviolet-induced oxidative stress, and reduce the oxidative degradation of collagen and elastic fibers; and the unsaturated fatty acids it contains can replenish the lipids between epidermal cells, repair the skin barrier, and enhance the water-locking ability.

[0011] The active ingredients contained in evening primrose can inhibit pro-inflammatory enzymes, reduce the release of PGE2, and block the activation of MMPs induced by oxidative stress; at the same time, the plant sterols and polyphenols contained in evening primrose root extract can inhibit the formation of advanced glycation end products, prevent collagen fiber cross-linking and hardening, and maintain skin elasticity and softness.

[0012] Furthermore, the composite extraction method of the sunflower extract and evening primrose root extract is:

[0013] Step 1: Add the roots of sunflower and evening primrose to 7-12 BV of 95% ethanol solution, stir and extract at 40±5°C for 3-4 hours, and centrifuge to obtain the extract;

[0014] Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract, stir and extract for 1 to 1.5 hours, and centrifuge to obtain the supernatant.

[0015] In the technical solution of the present invention, the step 2 can use conventional technology in the field to concentrate the extract into an extract; preferably, the specific conditions for concentration in the step 2 are: rotary evaporation at a temperature of 60°C, a pressure of -0.08~-0.1MPa, and a rotation speed of 60r / min.

[0016] Preferably, the fat-soluble solvent includes at least one of triethylhexanoin, caprylic / capric triglyceride, octyldodecanol, coconut oil caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and meadowfoam seed oil.

[0017] It should be noted that the fat-soluble solvent used in the technical solution of the present application mainly serves as a solvent and has no significant effect on the anti-wrinkle effect of the composition of the present application.

[0018] Preferably, the anti-wrinkle composition of the present invention further comprises Carthamus tinctorius flower extract.

[0019] Safflower extract is rich in unsaturated fatty acids, such as linoleic acid, which is a key component of the skin barrier. It replenishes intercellular lipids in the epidermis, repairs the stratum corneum, enhances shrinkage, and reduces fine lines caused by dryness. It also promotes keratinocyte differentiation, accelerates epidermal renewal, and improves skin roughness and texture. Furthermore, linoleic acid metabolites, including prostaglandin E1 (PGE1), inhibit the production of pro-inflammatory cytokines (PGE2), blocking the damage of inflammatory factors (such as IL-1β and IL-6) to fibroblasts. It also downregulates the NF-κB signaling pathway, reduces the activation of MMPs (collagen degrading enzymes), and protects the dermal matrix. Furthermore, it contains a small amount of fat-soluble safflower yellow pigment derivatives, which dilate capillaries, enhance blood oxygen and nutrient supply to the dermis, promote the removal of metabolic waste, and improve skin laxity and wrinkles.

[0020] More preferably, the anti-wrinkle composition of the present invention is obtained by composite extraction of sunflower seed, evening primrose root and safflower in a weight ratio of 2-8:3-9:5-20.

[0021] Furthermore, the composite extraction method of the sunflower extract, evening primrose root extract and safflower flower extract is:

[0022] Step 1: Add 7-12 BV of 95% ethanol solution to the sunflower, evening primrose root and safflower, stir and extract at 40±5°C for 3-4 hours, and centrifuge to obtain an extract;

[0023] Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract, stir and extract for 1 to 1.5 hours, and centrifuge to obtain the supernatant.

[0024] Preferably, the fat-soluble solvent includes at least one of triethylhexanoin, caprylic / capric triglyceride, octyldodecanol, coconut oil caprylate / caprate, ethylhexyl triazone, cetyl ethylhexanoate, and meadowfoam seed oil.

[0025] In the technical solution of the present invention, the step 2 can use conventional technology in the field to concentrate the extract into an extract; preferably, the specific conditions for concentration in the step 2 are: rotary evaporation at a temperature of 60°C, a pressure of -0.08~-0.1MPa, and a rotation speed of 60r / min.

[0026] The term "BV" as used herein stands for bed volume, abbreviated as BV. Resins are commonly used in chemical production, and solution is added to and passed through the resin column based on the amount of resin packed in the column. For example, 1 BV represents the amount of solution passed through 1 L of resin. This concept has been extended in the plant extraction industry and is often used to represent the weight of the extraction solvent added, calculated based on the weight of the raw material. It can also be referred to as a multiple. For example, 10 kg of water is added to 1 kg of raw material for extraction, which is recorded as 10 BV. This approach allows for a clear relationship between the amount of raw material input and the amount of solvent used during product development and production.

[0027] The invention also discloses the use of the anti-wrinkle composition in preparing skin care products.

[0028] The present invention also discloses a skin care product containing 0.01 to 50 wt % of the anti-wrinkle composition.

[0029] Beneficial effects

[0030] Compared with the prior art, the present invention provides an anti-wrinkle extract, which can synergistically enhance the skin anti-wrinkle effect of the composition by compounding the sunflower extract and the evening primrose root extract. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0032] Figure 1 This is a β-galactosidase staining image of human fibroblasts in the negative control group of the present invention;

[0033] Figure 2 This is a 0.1% human fibroblast β-galactosidase staining image of Example 4 of the present invention;

[0034] Figure 3 This is a diagram of a chicken embryo before use in Example 4 of the present invention;

[0035] Figure 4 This is a diagram of a chicken embryo after use in Example 4 of the present invention. DETAILED DESCRIPTION

[0036] The present invention will be further described below in conjunction with the embodiments, but this does not constitute any limitation to the present invention. Any limited modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0037] In order to explain the technical content of the present invention in detail, further description will be given below in conjunction with the embodiments.

[0038] In the following examples and comparative examples, the sunflower seed was purchased from Bozhou Pocket Doctor Biotechnology Co., Ltd., and the evening primrose root and safflower were both purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd.

[0039] Unless otherwise specified, in the following examples and comparative examples, parts and % refer to parts by weight and percentage by weight, respectively.

[0040] Example 1

[0041] An anti-wrinkle composition is obtained by the following steps:

[0042] Step 1: Add 5 parts of sunflower oil and 5 parts of evening primrose roots to 10 BV of 95% ethanol solution, stir and extract at 40±5°C for 4 hours, and centrifuge to obtain an extract;

[0043] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0044] Example 2

[0045] The method is generally the same as Example 1, except that the amounts of the sunflower and evening primrose roots are 7 parts and 3 parts, respectively.

[0046] Example 3

[0047] The method is generally the same as Example 1, except that the amounts of the sunflower and evening primrose roots are 3 parts and 9 parts, respectively.

[0048] Example 4

[0049] An anti-wrinkle composition is obtained by the following steps:

[0050] Step 1: Add 5 parts of sunflower oil, 5 parts of evening primrose root and 10 parts of safflower to 10BV of 95% ethanol solution, stir and extract at 40±5℃ for 4h, and centrifuge to obtain an extract;

[0051] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0052] Example 5

[0053] The method is generally the same as Example 4, except that the amounts of sunflower, evening primrose root and safflower are 2 parts, 4 parts and 18 parts respectively.

[0054] Comparative Example 1

[0055] A sunflower extract is obtained by the following steps:

[0056] Step 1: add 10 parts of sunflower oil to 10 BV of 95% ethanol solution, stir and extract at 40±5℃ for 4 hours, and centrifuge to obtain the extract;

[0057] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0058] Comparative Example 2

[0059] An evening primrose root extract is obtained by the following steps:

[0060] Step 1: Add 10 parts of evening primrose roots to 10 BV of 95% ethanol solution, stir and extract at 40±5°C for 4 hours, and centrifuge to obtain an extract;

[0061] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0062] Comparative Example 3

[0063] A safflower flower extract is obtained by the following steps:

[0064] Step 1: Add 10 parts of safflower to 10 BV of 95% ethanol solution, stir and extract at 40±5°C for 4 hours, and centrifuge to obtain the extract;

[0065] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0066] Comparative Example 4

[0067] An anti-wrinkle composition is obtained by the following steps:

[0068] Step 1: add 10 parts of sunflower oil and 10 parts of safflower oil to 10BV of 95% ethanol solution, stir and extract at 40±5℃ for 4h, and centrifuge to obtain the extract;

[0069] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0070] Comparative Example 5

[0071] An anti-wrinkle composition is obtained by the following steps:

[0072] Step 1: Add 10 parts of evening primrose root and 10 parts of safflower to 10 BV of 95% ethanol solution, stir and extract at 40±5°C for 4 hours, and centrifuge to obtain an extract;

[0073] Step 2: The extract was concentrated into an extract by rotary evaporation at a temperature of 60°C, a pressure of -0.09 MPa, and a speed of 60 r / min. 30 times the weight of the extract was added to the extract, and the mixture was stirred and extracted for 1 hour. The supernatant was obtained by centrifugation.

[0074] Efficacy testing

[0075] 1. Elastase inhibition rate

[0076] 1. Test purpose and principle

[0077] Anti-wrinkle efficacy is primarily characterized by evaluating the test sample's inhibition rate against elastase. Elastase, primarily synthesized and secreted by fibroblasts, degrades elastin in the skin, contributing to skin aging. The experimental principle for elastase inhibition is that porcine pancreatic elastase catalyzes a reaction with the enzyme substrate. Adding the active substance causes a change in absorbance, which reflects the inhibition rate of the elastase inhibitor.

[0078] 2 Test indicators

[0079] Criteria for determining the elastase inhibition rate: If the elastase inhibition rate of the sample is higher than that of the negative control, it can be considered that the test sample has a certain anti-wrinkle effect.

[0080] 3 Experimental materials and methods

[0081] 3.1 Instruments: BSA224S analytical balance, RT-6100 enzyme-labeled analyzer.

[0082] 3.2 Reagents: Elastase (porcine pancreas), BR; N-succinyl-L-alanyl-L-alanine, 98%; epigallocatechin gallate (EGCG), 98%.

[0083] 3.3 Test methods

[0084] (1) Treatment of control and test samples

[0085] Sample group: Examples 1 to 5 and Comparative Examples 1 to 5 diluted with anhydrous ethanol to a concentration of 5%;

[0086] Negative control: pure water.

[0087] (2) Test operation steps

[0088] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0089] (3) Calculation formula

[0090] ;

[0091] Where: A—is the absorbance of the reaction solution without sample and containing enzyme;

[0092] B—is the absorbance of the reaction solution without sample and enzyme;

[0093] C—is the absorbance of the reaction solution containing the sample and the enzyme;

[0094] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0095] The test was carried out according to the above method, and the results are shown in Table 1;

[0096] Table 1 Elastase inhibition rate results of Examples 1 to 5 and Comparative Examples 1 to 4

[0097] Group Elastase inhibition rate% Example 1 40.22 Example 2 35.48 Example 3 38.67 Example 4 49.32 Example 5 45.77 Comparative Example 1 11.13 Comparative Example 2 29.92 Comparative Example 3 15.79 Comparative Example 4 14.95 Comparative Example 5 19.75 Negative control 1.92

[0098] According to the results in Table 1, we can see that:

[0099] According to the data of Examples 1-3, the composite of the sunflower mallow extract and the evening primrose root extract obtained by the composite extraction of sunflower mallow and evening primrose root of the present invention can have a higher elastase inhibition rate; and according to the comparison of the data between Examples 1-3, it can be seen that sunflower mallow and evening primrose root are both important components, and it is not the case that one of them is the main component and the other plays a supporting role; the dosage ratio of sunflower mallow and evening primrose root will also have a certain impact on the elastase inhibition rate of the composition.

[0100] According to the comparison of the data of Example 4 and Example 1, the anti-wrinkle composition obtained by adding safflower to the compound extraction of sunflower and evening primrose root has a more significant elastase inhibition rate.

[0101] At the same time, according to the comparison of the data of Example 1, Example 4 and Comparative Examples 1-3, it can be seen that the present application produces a significant improvement in the elastase inhibition rate by compounding the sunflower extract and the evening primrose root extract, indicating that the sunflower extract and the evening primrose root extract have a synergistic effect of improving the elastase inhibition rate of the composition;

[0102] At the same time, in Example 4 of the present application, the addition of Carthamus tinctorius extract to the sunflower extract and the evening primrose root extract resulted in a more significant improvement in the elastase inhibition rate, which may be due to one of the following reasons:

[0103] (1) There is a significant synergistic effect between the extracts of Hibiscus sabdariffa and Carthamus tinctorius;

[0104] (2) There is a significant synergistic effect between evening primrose root extract and safflower flower extract;

[0105] (3) As a synergistic ingredient, safflower flower extract can further enhance the synergistic effect of sunflower extract and evening primrose root extract.

[0106] In order to verify the above reasons, the present application verified whether there is a synergistic effect between the sunflower extract and the Carthamus tinctorius flower extract, and between the evening primrose root extract and the Carthamus tinctorius flower extract through comparative examples 4 and 5 respectively;

[0107] According to the data comparison of Example 4 and Comparative Examples 1-5 in Table 1, it can be seen that:

[0108] (1) There is only a simple superposition of the effects between the extracts of Hibiscus sabdariffa and Carthamus tinctorius, or the synergistic effect between the two is not obvious;

[0109] (2) There is only a simple superposition of efficacy between evening primrose root extract and safflower flower extract, or the synergistic effect between the two is not obvious;

[0110] It can be seen that Example 4 of the present application produces a further synergistic effect by adding Carthamus tinctorius extract on the basis of Hibiscus sibiricum extract and Oenothera biennis root extract. Carthamus tinctorius extract, as a significant synergistic ingredient, further enhances the synergistic effect of Hibiscus sibiricum extract and Oenothera biennis root extract.

[0111] 2. Human fibroblast β-galactosidase staining experiment

[0112] 1. Experimental Purpose and Principle

[0113] Cellular senescence is a persistent state of cell cycle arrest that accumulates in aging organisms, and wrinkles are an outward manifestation of cellular senescence. Human fibroblasts were induced with hydrogen peroxide (H2O2) and β-galactosidase was used as a substrate. Senescent cells produce a dark blue product under the catalysis of β-galactosidase using X-Gal as a substrate.

[0114] Human fibroblasts can be used as a cell model to study the anti-wrinkle efficacy of cosmetics. The efficacy of the samples can be evaluated by comparing the degree of β-galactosidase staining between the negative control group cells and the sample group cells.

[0115] 2 Test indicators

[0116] The criteria for judging the degree of human fibroblast β-galactosidase staining are as follows: the lower the degree of human fibroblast β-galactosidase staining, the higher the efficacy of the sample and the better the anti-wrinkle effect.

[0117] 3 Experimental materials and methods

[0118] 3.1 Instruments: MF52-N inverted fluorescence microscope.

[0119] 3.2 Cells used in the experiment: human fibroblasts.

[0120] 3.3 Test methods

[0121] (1) Treatment of test samples (human fibroblast β-galactosidase staining test)

[0122] Sample group: basal culture medium containing H2O2 and 0.1% sample.

[0123] Negative control: basal culture medium containing H2O2.

[0124] (2) Test operation steps

[0125] Human fibroblast β-galactosidase staining assay: Human fibroblasts were plated in a 6-well plate and cultured for 24 hours. β-galactosidase fixative was added and fixed at room temperature for 15 minutes. β-galactosidase working solution was then added to each well and incubated at 37°C overnight. Blue-stained cells were observed under a standard optical microscope.

[0126] (3) Data analysis

[0127] Quantitative analysis of the staining images was performed using analysis software.

[0128] Example 4 was tested according to the above test method, and the results are shown in Table 2 and Figure 1-2 As shown, improvement rate % = (staining degree of negative control - staining degree of sample) / staining degree of negative control * 100%; Figure 1 This is the β-galactosidase staining picture of human fibroblasts in the negative control group. Figure 2 This is a 0.1% β-galactosidase staining image of human fibroblasts in Example 4.

[0129] Table 2 Results of human fibroblast β-galactosidase staining experiment in Example 4

[0130] Group Degree of dyeing Improvement rate% Example 4 6.87 66.22 Negative control 20.33 /

[0131] According to the results in Table 2, we can see that:

[0132] Example 4 of the present application has significant skin anti-wrinkle effect.

[0133] 3. Stimulation Experiment

[0134] Test method: Chicken embryo chorioallantoic membrane test

[0135] 1. Purpose and Principle of the Test

[0136] The chick chorioallantoic membrane test is an early in vitro method for evaluating eye irritation. The chorioallantoic membrane (CAM) is a respiratory membrane that surrounds the chick embryo. This test utilizes the intact, clear, and transparent vascular system of the chorioallantoic membrane of mid-stage incubated chick embryos. A certain amount of the test substance is directly exposed to the CAM. After a period of exposure, changes in toxic effect indicators (such as bleeding, coagulation, and vascular melting) are observed. These indicators reflect changes in the morphology, structure, color, and permeability of the blood vessels and vascular network, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. These indicators are then combined to generate a score used to assess the eye irritation potential of the test substance.

[0137] The purpose of this test is to test the ability of the test substance to cause toxic changes in the chicken embryo chorioallantoic membrane and to evaluate the factors and processes of the potential eye irritation of the evaluated substance.

[0138] 2. Experimental Group Treatment and Reagents

[0139] Sample group: Example 4 was diluted into a 2% oil solution.

[0140] Negative control: 0.9% saline.

[0141] Positive control: 1% sodium dodecyl sulfate (SDS) solution.

[0142] 3. Experimental steps

[0143] Six chick embryos were used in each group for this test. The chorioallantoic membrane (CAM) was recorded using a camera. A polytetrafluoroethylene (PTFE) ring was placed on the CAM and photographed. The sample to be tested was added to the ring, the time of sample addition was recorded, and the air chamber was covered with moistened plastic wrap. The embryos were then transferred to a constant temperature and humidity chamber for incubation, and the extent of each toxic effect was observed.

[0144] 4. Calculation of results

[0145] The endpoint evaluation method was used to calculate the endpoint evaluation (ES) with the result rounded to two decimal places. Each chicken embryo was scored as the sum of the observed bleeding, coagulation and vascular lysis. The mathematical sums obtained for ES-6 chicken embryos were averaged.

[0146] The average ES score is calculated using the following formula:

[0147] .

[0148] 5. Result judgment criteria

[0149] ES≤4, non-irritating;

[0150] 4<ES≤12, mild irritation;

[0151] 12<ES<16, moderate irritation;

[0152] ES ≥ 16, strong irritant / corrosive.

[0153] Test results

[0154] The sample of Example 4 was tested according to the above test method, and the ES value was 2.00, which was determined to be non-irritating.

[0155] Example 4 The results of chicken embryos before and after use are as follows Figure 3 、 Figure 4 As shown, it is proved that the technical solution of the present invention is mild and non-irritating.

[0156] Application Examples

[0157] An anti-wrinkle product, the formula is shown in Table 3:

[0158] Table 3 Formula of anti-wrinkle products

[0159]

[0160] An anti-wrinkle product, the formula is shown in Table 3, and the specific preparation method is as follows:

[0161] Step 1: Preheat phase A to 80-85°C to dissolve, stir and mix evenly, and set aside;

[0162] Step 2: Add the ingredients in phase B to the main pot in sequence and stir evenly;

[0163] Step 3: Add phase A and phase C and stir well.

[0164] The embodiments presented herein are merely embodiments selected from a combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include subranges therein, and variations in these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle composition, characterized in that It is composed of sunflower extract, evening primrose root extract and safflower flower extract, which is obtained by compound extraction of sunflower, evening primrose root and safflower in a weight ratio of 2~8:3~9:5~20; The composite extraction method of the sunflower extract, evening primrose root extract and safflower flower extract is as follows: Step 1: Add 7-12 BV of 95% ethanol solution to the sunflower, evening primrose root and safflower, stir and extract at 40±5°C for 3-4 hours, and centrifuge to obtain an extract; Step 2: Concentrate the extract into an extract, add a fat-soluble solvent to the extract, stir and extract for 1 to 1.5 hours, and centrifuge to obtain the supernatant.

2. Use of the anti-wrinkle composition according to claim 1 in preparing skin care products.

3. A skin care product, characterized in that: Contains 0.01 to 50 wt % of the anti-wrinkle composition according to claim 1.

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