Preparation process of improved fructus forsythiae extract

Abstract

The invention provides a preparation process of an improved fructus forsythiae extract, and relates to the technical field of preparation of fructus forsythiae extracts. The preparation process of the improved fructus forsythiae extract comprises the following specific steps: S1, firstly, cleaning a fructus forsythiae raw material with clear water, and putting the fructus forsythiae raw material in an environment of long normal temperature for natural airing; s2, putting the aired fructus forsythiae raw material into a container, and then adding a rosmarinic acid solution into the container for soaking. The fructus forsythiae raw material is soaked in a rosmarinic acid solution when being treated, so that the fructus forsythiae raw material is conveniently subjected to bacteriostasis, the influence of bacteria, fungi and mould contained in the fructus forsythiae raw material on the fructus forsythiae extract is avoided, and when the fructus forsythiae extract is prepared, a Tris-HCI buffer solution is added, so that the fructus forsythiae raw material is effectively prevented from being degraded. The molecular structure in the fructus forsythiae extract can be protected, so that active molecules in the fructus forsythiae extract are prevented from being easily inactivated in acidity or alkalinity when the extraction solvent is added.

Description

Technical Field

[0001] The present invention relates to the technical field of forsythia extract preparation, and specifically to an improved preparation process for forsythia extract. Background Art

[0002] Traditional Chinese medicine mainly originated in China and is a medicine used for preventing, diagnosing, treating diseases or regulating human body functions under the guidance of traditional Chinese medicine theory. Most of them are plant medicines, and there are also animal medicines, mineral medicines and some chemical and biological product medicines. Traditional Chinese medicine is divided into patent medicines and Chinese medicinal materials according to the processing technology. For Chinese medicinal materials, how to efficiently extract their effective pharmaceutical ingredients is one of the current research hotspots. Among them, forsythia has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and is one of the commonly used traditional Chinese medicines in Chinese clinical practice, often used to treat acute wind-heat colds, lymph node tuberculosis and other diseases.

[0003] At present, various extraction solvents will be added during the preparation of forsythia extract, but the addition of various solvents will make the solvent acidic or alkaline, and the active molecules in the forsythia extract are prone to lose their activity in an acidic or alkaline environment, thus affecting the reduction of the effective ingredients of the forsythia extract. Summary of the Invention

[0004] (1) Technical Problems to be Solved

[0005] In view of the deficiencies of the prior art, the present invention provides an improved preparation process for forsythia extract, which solves the problem that various extraction solvents are added during the preparation of forsythia extract, and the addition of various solvents will make the solvent acidic or alkaline, and the active molecules in the forsythia extract are prone to lose their activity in an acidic or alkaline environment, thus affecting the reduction of the effective ingredients of the forsythia extract.

[0006] (2) Technical Solutions

[0007] To achieve the above objectives, the present invention is realized through the following technical solutions: an improved preparation process for forsythia extract, including the following specific steps:

[0008] S1. First, wash the forsythia raw materials with clean water and place them in a normal temperature environment for natural air drying;

[0009] S2. Place the air-dried forsythia raw materials in a container, and then add rosmarinic acid solution to the container for soaking;

[0010] S3. After the forsythia raw materials are soaked, they are air-dried, and then the forsythia raw materials are crushed using a crushing device and screened using a 100-200 mesh sieve to obtain forsythia powder for standby;

[0011] S4. Add the forsythia powder obtained in S3 to a container, then add Tris-HCI buffer solution to the container, and then stir evenly using a stirring rod;

[0012] S5. Add sodium citrate solution and calcium chloride solution into the container successively, stir evenly with a stirring rod, and let it stand for precipitation. After the standing precipitation is completed, add ethanol to the container again to resuspend the precipitate.

[0013] S6. Filter out the precipitate in S5 to obtain forsythia extract solution, and finally inject the obtained forsythia extract solution into a spray dryer for drying treatment to obtain forsythia extract powder.

[0014] Preferably, the mass parts of the forsythia raw material, rosmarinic acid solution, Tris-HCl buffer solution, sodium citrate solution, calcium chloride solution and ethanol are respectively: 10-30 parts of forsythia raw material, 10-15 parts of rosmarinic acid solution, 6-7 parts of Tris-HCl buffer solution, 10-13 parts of sodium citrate solution, 8-12 parts of calcium chloride solution, 5-9 parts of ethanol and 20-40 parts of water.

[0015] Preferably, the forsythia raw material in S2 needs to be cut into pieces and the soaking time is 10-15 minutes.

[0016] Preferably, the stirring methods in S4 and S5 both adopt the method of alternating forward stirring and reverse stirring, and the stirring time is 5-8 min.

[0017] Preferably, the standing precipitation time in S5 is 8-10 min.

[0018] Preferably, the temperature of the spray dryer in S6 during use is 100-120 °C.

[0019] (III) Beneficial effects

[0020] The present invention provides an improved preparation process of forsythia extract, having the following beneficial effects:

[0021] 1. In the present invention, when treating the forsythia raw material, by soaking the forsythia raw material in the rosmarinic acid solution, it is convenient to inhibit bacteria on the forsythia raw material and avoid the influence of bacteria, fungi and molds contained in the forsythia raw material on the forsythia extract.

[0022] 2. In the present invention, when preparing the forsythia extract, by adding Tris-HCl buffer solution, the molecular structure in the forsythia extract can be protected, and it is avoided that the active molecules in the forsythia extract lose their activity in an acidic or alkaline environment while adding the extraction solvent.

[0023] 3. In the present invention, during the drying process, by using a spray dryer for drying, it is beneficial to improve the drying efficiency of the extract, reduce the decomposition and loss of active ingredients, and make the final extract have better efficacy. Detailed implementation manners

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0025] Embodiment:

[0026] The embodiment of the present invention provides an improved preparation process of forsythia extract, including the following specific steps:

[0027] S1. First, clean the forsythia raw materials with clean water and place them in a normal temperature environment for natural air drying. This cleaning mainly removes impurities such as dust attached to the forsythia raw materials;

[0028] S2. Place the air-dried forsythia raw materials in a container, and then add rosmarinic acid solution to the container for soaking. The rosmarinic acid solution mainly plays an antibacterial role to prevent bacteria, fungi, and molds contained in the forsythia raw materials from affecting the forsythia extract;

[0029] S3. After the forsythia raw materials are soaked, they are air-dried, and then the forsythia raw materials are crushed using a crushing device and screened using a 100-200 mesh sieve to obtain forsythia powder for standby. The crushed forsythia powder can fully contact with the extraction solvent, and the extraction effect is better;

[0030] S4. Add the forsythia powder obtained in S3 to a container, then add Tris-HCI buffer solution to the container, and then stir evenly with a stirring rod. By adding Tris-HCI buffer solution, the molecular structure in the forsythia extract can be protected to prevent the active molecules in the forsythia extract from losing activity in an acidic or alkaline environment when adding the extraction solvent;

[0031] S5. Add sodium citrate solution and calcium chloride solution to the container successively, stir evenly with a stirring rod, and let it stand for precipitation. After the standing precipitation is completed, add ethanol to the container again to resuspend the precipitate;

[0032] S6. Filter out the precipitate in S5 to obtain the forsythia extract solution. Finally, inject the obtained forsythia extract solution into a spray dryer for drying treatment to obtain forsythia extract powder. By using a spray dryer for drying, it is beneficial to improve the drying efficiency of the extract, reduce the decomposition and loss of active ingredients, and make the final extract have better efficacy.

[0033] The spray dryer utilizes spray technology and hot air flow technology. It sprays the forsythia extract in liquid form into the drying chamber, and through the action of the hot air flow, the moisture is quickly evaporated, thus obtaining the dried forsythia extract powder. Its working process can be briefly summarized as the following steps:

[0034] 1. Liquid spraying: The forsythia extract is sprayed into tiny droplets through a nozzle. These droplets have a large surface area, which is conducive to the evaporation of moisture and the progress of the drying process.

[0035] 2. Action of hot air flow: The hot air flow passes through the spraying chamber, contacts the sprayed droplets, and quickly evaporates the moisture in the droplets. The temperature and flow rate of the hot air flow need to be adjusted according to the specific forsythia extract to ensure the effect and quality of the drying process.

[0036] 3. Collection of dried powder: The evaporated moisture will be carried away together with the forsythia extract. The water vapor is discharged through the exhaust gas system of the drying chamber, while the dried forsythia extract powder is collected.

[0037] The mass parts of forsythia raw materials, rosmarinic acid solution, Tris-HCI buffer solution, sodium citrate solution, calcium chloride solution, and ethanol are as follows: 30 parts of forsythia raw materials, 15 parts of rosmarinic acid solution, 7 parts of Tris-HCI buffer solution, 13 parts of sodium citrate solution, 12 parts of calcium chloride solution, 9 parts of ethanol, and 40 parts of water. In S2, the forsythia raw materials need to be cut into pieces and the soaking time is 15 minutes. The stirring methods in S4 and S5 both adopt the alternating stirring method of forward stirring and reverse stirring, and the stirring time is 8 minutes each. The standing precipitation time in S5 is 10 minutes. The temperature of the spray dryer during use in S6 is 110°C.

[0038] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An improved preparation process of forsythia extract, characterized in that: It includes the following specific steps: S1. First, wash the forsythia raw materials with clean water and place them in a normal temperature environment for natural air drying; S2. Place the air-dried forsythia raw materials in a container, and then add rosmarinic acid solution to the container for soaking; S3. After the forsythia raw materials are soaked, air-dry them, then use a pulverizing device to pulverize the forsythia raw materials, and use a 100-200 mesh sieve for screening to obtain forsythia powder for standby; S4. Add the forsythia powder obtained in S3 to a container, then add Tris-HCI buffer solution to the container, and then stir evenly with a stirring rod; S5. Add sodium citrate solution and calcium chloride solution to the container successively, stir evenly with a stirring rod, and let it stand for precipitation. After the standing precipitation is completed, add ethanol to the container again to resuspend the precipitate; S6. Filter out the precipitate in S5 to obtain a forsythia extract solution. Finally, inject the obtained forsythia extract solution into a spray dryer for drying treatment to obtain forsythia extract powder.

2. An improved preparation process of forsythia extract according to claim 1, characterized in that: The mass parts of the forsythia raw materials, rosmarinic acid solution, Tris-HCI buffer solution, sodium citrate solution, calcium chloride solution and ethanol are respectively: 10-30 parts of forsythia raw materials, 10-15 parts of rosmarinic acid solution, 6-7 parts of Tris-HCI buffer solution, 10-13 parts of sodium citrate solution, 8-12 parts of calcium chloride solution, 5-9 parts of ethanol and 20-40 parts of water.

3. An improved preparation process of forsythia extract according to claim 1, characterized in that: In S2, the forsythia raw materials need to be cut into pieces, and the soaking time is 10-15 minutes.

4. An improved preparation process of forsythia extract according to claim 1, characterized in that: The stirring methods in S4 and S5 both adopt the method of alternating forward stirring and reverse stirring, and the stirring time is 5-8 min.

5. An improved preparation process of forsythia extract according to claim 1, characterized in that: The standing precipitation time in S5 is 8-10 min.

6. An improved preparation process of forsythia suspense extract according to claim 1, characterized in that: The temperature of the spray dryer in S6 during use is 100-120 °C.