A traditional Chinese medicine fermentation composition with the function of tonifying qi and blood and a preparation method thereof

Abstract

The application discloses a traditional Chinese medicine fermentation composition with the functions of tonifying qi and blood and a preparation method thereof, and belongs to the technical field of health products. The traditional Chinese medicine fermentation composition is prepared from the following raw materials in parts by weight: radix codonopsis 15-20 parts, angelica 12-18 parts, longan arillus 5-10 parts, spironast 3-7 parts, yellow mustard seed 2-6 parts, amomum 4-9 parts and radix asparagi 5-11 parts. The traditional Chinese medicine fermentation composition is prepared through the synergistic compatibility of various traditional Chinese medicines, an efficient extraction process and a multi-strain synergistic fermentation process, can significantly improve the functions of tonifying qi and blood of the traditional Chinese medicine fermentation composition, and provides an innovative solution for the industrialized production of health products for tonifying qi and blood.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health products, more particularly to a traditional Chinese medicine fermentation composition with the function of tonifying qi and blood and a preparation method thereof. BACKGROUND

[0002] With the increase of life rhythm and life pressure, most people are in a sub-health state, among which many are qi and blood deficiency.

[0003] Most traditional Chinese medicine qi and blood tonifying compositions adopt single-step extraction or single-strain fermentation process, and have the problems of low conversion rate of active ingredients and insufficient synergistic effect of medicinal ingredients. For example, the content of cycloastragenol as a key active ingredient is easily affected by raw material compatibility, extraction process and microbial fermentation conditions. The content of active ingredients and the synergistic effect of medicinal ingredients in the qi and blood tonifying composition determine its qi and blood tonifying effect. If the raw material compatibility is unreasonable, the extraction process or the microbial fermentation process is not suitable, the qi and blood tonifying effect will be affected, resulting in low trust of consumers in qi and blood tonifying health products. SUMMARY

[0004] The present application aims to overcome the deficiencies of the prior art, provide a traditional Chinese medicine fermentation composition with the function of tonifying qi and blood and a preparation method thereof, significantly improve the qi and blood tonifying effect of the traditional Chinese medicine fermentation composition, and provide an innovative solution for the industrialized production of qi and blood tonifying health products.

[0005] In order to achieve the above-mentioned purpose, the present application adopts the following technical solutions:

[0006] A traditional Chinese medicine fermentation composition with the function of tonifying qi and blood, which is made of raw materials including the following weight parts: radix codonopsis 15-20 parts, angelica 12-18 parts, longan arillus 5-10 parts, spironolactone 3-7 parts, yellow mustard seed 2-6 parts, amomum 4-9 parts, and monkshood 5-11 parts.

[0007] The present application further provides that the traditional Chinese medicine fermentation composition is made of raw materials including the following weight parts: radix codonopsis 18.6 parts, angelica 15 parts, longan arillus 7.5 parts, spironolactone 4.9 parts, yellow mustard seed 3.3 parts, amomum 6 parts, and monkshood 8.7 parts.

[0008] A preparation method of a traditional Chinese medicine fermentation composition with the function of tonifying qi and blood, comprising the following steps:

[0009] (1) Mix radix codonopsis, amomum, monkshood and angelica and crush them to 60-100 mesh, then mix the crushed powder with an ethanol aqueous solution and heat extract, heat concentrate the extract to 1 / 4 of the original volume, and obtain a mixed concentrate;

[0010] (2) mix purslane, longan meat and water to make pulp, then put the pulp in 40℃ water bath environment for 1-2h to obtain mixed pulp;

[0011] (3) add pulverized Semen Ranunculi powder with granularity of 60-100 to water, and put it in 40-45℃ for 2-3h to obtain Semen Ranunculi slurry;

[0012] (4) mix the mixed concentrate, mixed pulp and Semen Ranunculi slurry, sterilize, inoculate Bacillus subtilis and Monascus for aerobic fermentation, then inoculate Lactobacillus for anaerobic fermentation, filter the fermentation product after fermentation, vacuum dry the product, and sterilize by ultraviolet irradiation to obtain the traditional Chinese medicine fermentation composition.

[0013] The application is further provided that the extraction in step (1) is a three-stage extraction, i.e., first extraction at 50-60℃ for 20-30min, then extraction at 70-75℃ for 40-45min, and finally extraction at 85℃ for 15-20min.

[0014] The application is further provided that the ratio of material to liquid is 1:8-10 during extraction in step (1), and the concentration of the ethanol aqueous solution is 50wt%.

[0015] The application is further provided that the content rate of the mixed pulp is 20-30% in step (2).

[0016] The application is further provided that the water content rate of the Semen Ranunculi slurry is 30-40% in step (3).

[0017] The application is further provided that the Bacillus subtilis is Bacillus subtilis DE111, and the Lactobacillus is Lactobacillus delbrueckii subsp. bulgaricus in step (4).

[0018] The application is further provided that the inoculation amount of the Bacillus subtilis is 5-6%, the inoculation amount of the Monascus is 3-4%, and the inoculation amount of the Lactobacillus is 4-5% in step (4).

[0019] The application is further provided that the aerobic fermentation is carried out at 35-37℃ for 40-48h, the fermentation is stirred every 8h, the oxygen concentration is controlled at 10-15% during the fermentation, and the anaerobic fermentation is carried out at 30-37℃ for 30-36h in step (4).

[0020] In summary, the application has the following beneficial effects:

[0021] By the synergistic combination of main drugs such as radix codonopsis and angelica with spilanthes acmella and sinapis arvensis, combined with three-stage gradient temperature extraction and multi-strain synergistic fermentation process, the dissolution rate of active ingredients in medicinal materials is significantly improved, efficient conversion and stable accumulation of active ingredients are realized, the qi and blood tonifying effect of the traditional Chinese medicine fermented composition is significantly improved, and an innovative solution is provided for the industrialized production of qi and blood tonifying health products. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0023] The traditional Chinese medicine fermented composition with the function of tonifying qi and blood is made of raw materials including the following weight parts: radix codonopsis 15-20 parts, angelica 12-18 parts, longan meat 5-10 parts, spilanthes acmella 3-7 parts, sinapis arvensis 2-6 parts, amomum villosum 4-9 parts, and asparagus 5-11 parts. The preparation method is as follows:

[0024] (1) Mix and crush radix codonopsis, amomum villosum, asparagus, and angelica to 60-100 mesh, and then mix the mixed powder with 50wt% ethanol aqueous solution (solid-liquid ratio of 1:8-10), and then heat extraction (three-stage extraction: first extraction at 50-60℃ for 20-30min, then extraction at 70-75℃ for 40-45min, and then extraction at 85℃ for 15-20min), and then heat concentration of the extraction liquid to 1 / 4 of the original volume to obtain a mixed concentrate;

[0025] (2) Mix and beat spilanthes acmella, longan meat, and water to obtain a mixed slurry (content rate of 20-30%) by standing in a 40℃ water bath environment for 1-2h;

[0026] (3) Add sinapis arvensis powder crushed to 60-100 mesh to water, and then stand at 40-45℃ for 2-3h to obtain sinapis arvensis slurry (water content rate of 30-40%);

[0027] (4) the mixed concentrate, the mixed slurry, the prunus heterophylla slurry are mixed and sterilized, inoculated into bacillus subtilis (preferably bacillus subtilis DE111) and monascus for aerobic fermentation at 35-37°C for 40-48h, the inoculation amount of bacillus subtilis is 5-6%, the inoculation amount of monascus is 3-4%, the fermentation is stirred every 8h, and the oxygen concentration is controlled at 10-15% during the fermentation; then inoculated into lactobacillus (preferably lactobacillus delbrueckii bulgarian) for anaerobic fermentation at 30-37°C for 30-36h, the inoculation amount of lactobacillus is 4-5%, and the fermentation product is filtered after the fermentation is completed, the product is vacuum dried, and then ultraviolet radiation sterilization is performed to obtain the traditional Chinese medicine fermentation composition.

[0028] Example 1

[0029] (1) the following mass parts of raw materials are prepared: radix codonopsitis 18.6 parts, angelica 15 parts, longan meat 7.5 parts, spironast 4.9 parts, prunus heterophylla 3.3 parts, amomum villosum 6 parts, and monkshood 8.7 parts.

[0030] (2) radix codonopsitis, amomum villosum, monkshood, and angelica are mixed and crushed to 60-100 meshes, and the mixed powder is mixed with an ethanol aqueous solution with a concentration of 50wt% (the solid-liquid ratio is 1:8) and then heated for extraction (three-stage extraction is adopted: first extraction at 60°C for 20min, then extraction at 72°C for 45min, and finally extraction at 85°C for 15min), and the extract is concentrated to 1 / 4 of the original volume to obtain a mixed concentrate;

[0031] (3) spironast, longan meat, and water are mixed and slurried, and then the slurry is placed in a 40°C water bath environment for 1.5h to obtain a mixed slurry (the content rate is controlled to be 25%);

[0032] (4) prunus heterophylla powder crushed to 60-100 meshes is added to water, and the mixture is placed at 40°C for 2h to obtain a prunus heterophylla slurry (the water content rate is controlled to be 30%);

[0033] (5) the mixed concentrate, the mixed slurry, and the prunus heterophylla slurry are mixed and sterilized, inoculated into bacillus subtilis DE111 and monascus for aerobic fermentation at 37°C for 48h, the inoculation amount of bacillus subtilis is 5.1%, the inoculation amount of monascus is 3.6%, the fermentation is stirred every 8h, and the oxygen concentration is controlled at 12% during the fermentation; then inoculated into lactobacillus delbrueckii bulgarian for anaerobic fermentation at 35°C for 30h, the inoculation amount of lactobacillus is 4%, and the fermentation product is filtered after the fermentation is completed, the product is vacuum dried, and then ultraviolet radiation sterilization is performed to obtain the traditional Chinese medicine fermentation composition.

[0034] Comparative examples 1-4

[0035] The traditional Chinese medicine fermentation composition was prepared according to the method of Example 1, except that: no spilanthes acmella was added in Comparative Example 1, no Semen Raphani was added in Comparative Example 3, no Amomum kravanh was added in Comparative Example 3, and no Asparagus cochinchinensis was added in Comparative Example 4.

[0036] Comparative Example 5

[0037] The traditional Chinese medicine fermentation composition was prepared according to the method of Example 1, except that: no Monascus was added for fermentation.

[0038] Comparative Example 6

[0039] The traditional Chinese medicine fermentation composition was prepared according to the method of Example 1, except that: no Bacillus subtilis DE111 was added for fermentation.

[0040] Comparative Example 7

[0041] The traditional Chinese medicine fermentation composition was prepared according to the method of Example 1, except that: no Lactobacillus delbrueckii subsp. bulgaricus was subjected to anaerobic fermentation.

[0042] The traditional Chinese medicine fermentation compositions prepared in Example 1, Comparative Examples 1-7 were respectively subjected to cycloastragenol saponin content testing. It was found that only the content of the traditional Chinese medicine fermentation composition in Example 1 was more than 4.3%, and the contents of the traditional Chinese medicine fermentation compositions in Example 1, Comparative Examples 5-7 were 5.2%, 4.0%, 3.1%, and 3.7%, respectively.

[0043] Pharmacodynamic animal test of qi and blood tonifying

[0044] Experimental animals: SD rats, body weight 220±5g, half male and half female, SPF level, and all female rats were not pregnant.

[0045] Construction of blood deficiency rat model: the rats were randomly divided into 10 groups, 10 rats in each group; group 1 was administered with the traditional Chinese medicine fermentation composition of Example 1; group 2 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 1; group 3 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 2; group 4 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 3; group 5 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 4; group 6 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 5; group 7 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 6; group 8 was administered with the traditional Chinese medicine fermentation composition of Comparative Example 7; group 9, model group, was administered with the same volume of normal saline; group 10, blank control group, was administered with the same volume of normal saline. Before the experiment, except for the blank control group, the other groups were subcutaneously injected with acetylphenylhydrazine 10, 20mg / kg on the 2nd and 5th days, and the administration group was administered 30 minutes after injection; from the 5th day, intraperitoneal injection of cyclophosphamide 20mg / kg was performed for 4 consecutive days, and the administration group was administered 30 minutes after injection. On the 1st day of the formal experiment, normal saline and the traditional Chinese medicine fermentation composition were administered, continuously for 15 days, and each group of rats was administered twice a day, and each time 0.5g of the traditional Chinese medicine fermentation composition was dispersed in 200ml of water for administration in groups 1-8.

[0046] The results show that the administration group has the effect of increasing the decreased hemoglobin of the rats (P<0.05), and after 15 days of administration, the average hemoglobin content in the blood of the rats in group 1 to group 10 is 108.7 g / L, 90.1 g / L, 95.5 g / L, 94.3 g / L, 97.4 g / L, 85.2 g / L, 83.4 g / L, 78.5 g / L, 65.9 g / L (model group), and 156.4 g / L (control group) in turn.

[0047] The above is only the preferred embodiment of the present application, and the protection scope of the present application is not limited to the above-mentioned embodiments only, and any technical scheme falling within the concept of the present application shall fall within the protection scope of the present application. It should be noted that, for ordinary skilled persons in the art, some improvements and refinements without departing from the principles of the present application shall also be considered as the protection scope of the present application.

Claims

1. A method for preparing a fermented traditional Chinese medicine composition with the function of nourishing qi and blood, characterized in that, Includes the following steps: (1) Mix and pulverize Codonopsis pilosula, Amomum tsao-ko, Asparagus cochinchinensis and Angelica sinensis to 60-100 mesh. Mix the mixed powder of Codonopsis pilosula, Amomum tsao-ko, Angelica sinensis and Asparagus cochinchinensis with an ethanol aqueous solution and heat to extract. Heat and concentrate the extract to 1 / 4 of the original volume to obtain a mixed concentrate. (2) Mix purslane, longan pulp and water to make a paste, and then let the paste stand in a 40℃ water bath for 1-2 hours to obtain a mixed paste; (3) Add the yellow mustard powder, which has been pulverized to 60-100 mesh, to water and let it stand at 40-45℃ for 2-3 hours to obtain yellow mustard slurry; (4) After mixing the concentrated mixture, mixed slurry, and yellow mustard slurry, sterilize them, inoculate Bacillus subtilis and Monascus purpureus for aerobic fermentation, then inoculate Lactobacillus for anaerobic fermentation, filter the fermentation product after fermentation, vacuum dry the product and sterilize it by ultraviolet irradiation to obtain the traditional Chinese medicine fermentation composition. This traditional Chinese medicine fermentation composition is made from the following raw materials in parts by weight: 15-20 parts of Codonopsis pilosula, 12-18 parts of Angelica sinensis, 5-10 parts of longan pulp, 3-7 parts of Portulaca oleracea, 2-6 parts of Sinapis alba, 4-9 parts of Amomum tsao-ko, and 5-11 parts of Asparagus cochinchinensis.

2. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, This traditional Chinese medicine fermentation composition is made from the following raw materials in parts by weight: Codonopsis pilosula 18.6 parts, Angelica sinensis 15 parts, longan pulp 7.5 parts, purslane 4.9 parts, yellow mustard seed 3.3 parts, amomum tsao-ko 6 parts, and asparagus root 8.7 parts.

3. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (1), a three-stage extraction method is used: first, extract at 50-60℃ for 20-30 min, then extract at 70-75℃ for 40-45 min, and then extract at 85℃ for 15-20 min.

4. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (1), the material-to-liquid ratio during extraction is 1:8-10; the concentration of the ethanol-water solution is 50wt%.

5. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (2), the content of the mixed slurry is 20-30%.

6. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (3), the moisture content of the yellow mustard slurry is 30-40%.

7. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (4), the Bacillus subtilis is Bacillus subtilis DE111 and the Lactobacillus is Lactobacillus delbrueckii subsp. Bulgaria.

8. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (4), the inoculation amount of Bacillus subtilis is 5-6%; the inoculation amount of Monascus purpureus is 3-4%; and the inoculation amount of Lactobacillus is 4-5%.

9. The method for preparing a traditional Chinese medicine fermentation composition with qi and blood nourishing function according to claim 1, characterized in that, In step (4), aerobic fermentation is carried out at 35-37°C for 40-48 hours, with the mixture turned over every 8 hours during fermentation, and the oxygen concentration is controlled at 10-15% during fermentation; anaerobic fermentation is carried out at 30-37°C for 30-36 hours.