An apparatus and method for producing an infection-inhibiting indwelling needle

Abstract

The application discloses a production device and a production method of an indwelling needle for inhibiting infection, and belongs to the technical field of indwelling needle anti-infection, and aims to solve the problems of short active duration and poor efficiency of the indwelling needle for inhibiting infection in the prior art. The production device comprises a first etching unit and a coating unit arranged in sequence. The coating unit comprises a ring-shaped brush, a brush mounting ring, a liquid pipeline and a liquid supply cavity. The brush mounting ring is a hollow structure. The liquid outlet end of the liquid supply cavity is connected with the liquid inlet end of the liquid pipeline. The liquid outlet end of the liquid pipeline is connected with the liquid inlet port of the brush mounting ring. The ring-shaped brush is arranged on the inner wall of the brush mounting ring and is in contact with the liquid outlet port of the brush mounting ring. The liquid supply cavity is internally provided with, from bottom to top, an organic solvent solution of a first infection inhibiting layer material, an organic solvent solution of a second infection inhibiting layer material and an organic solvent solution of a third infection inhibiting layer material. The application can be used for the production of the indwelling needle for inhibiting infection.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of indwelling needle anti-infection, and particularly relates to a production device and a production method of an indwelling needle for inhibiting infection. BACKGROUND

[0002] As a widely used medical device in clinical intravenous infusion treatment, the indwelling needle can achieve multiple drug delivery by leaving the hose in the blood vessel, thereby avoiding the pain and blood vessel damage of the patient caused by repeated puncture. With the extension of the indwelling time (usually 72-96 hours), bacteria are easily adhered to the surface of the indwelling needle and form a biofilm, which becomes an important risk factor for causing indwelling needle-related bloodstream infections.

[0003] At present, the main way to inhibit infection of the indwelling needle is to coat the surface with an antibacterial agent, such as chlorhexidine, silver ions, rifampicin or minocycline, to inhibit bacterial proliferation by using the slow-release effect.

[0004] However, the inhibiting infection activity of the antibacterial agent coating lasts for a short time, and the inhibiting infection efficiency will decrease significantly as the coating falls off or the drug is released. SUMMARY

[0005] In view of the above analysis, the application aims to provide a production device and a production method of an indwelling needle for inhibiting infection, so as to solve the problems of short active duration and poor efficiency of the indwelling needle for inhibiting infection in the prior art.

[0006] The main purpose of the application is achieved by the following technical solutions.

[0007] In a first aspect, the application provides a production device of an indwelling needle for inhibiting infection, the indwelling needle for inhibiting infection comprising a puncture needle and an indwelling tube sleeved on the outer wall of the puncture needle, the indwelling tube comprising a base layer and a first inhibiting infection layer, a second inhibiting infection layer and a third inhibiting infection layer formed in sequence on the outer wall of the base layer; the production device comprising a first etching unit and a coating unit arranged in sequence; the coating unit comprising a ring-shaped brush, a brush mounting ring, a liquid pipeline and a liquid supply cavity, the brush mounting ring being a hollow structure, the liquid outlet end of the liquid supply cavity being connected with the liquid inlet end of the liquid pipeline, the liquid outlet end of the liquid pipeline being connected with the liquid inlet port of the brush mounting ring, and the ring-shaped brush being arranged on the inner wall of the brush mounting ring and being in contact with the liquid outlet port of the brush mounting ring.

[0008] Further, the first inhibiting infection layer material organic solvent solution, the second inhibiting infection layer material organic solvent solution and the third inhibiting infection layer material organic solvent solution are arranged in the liquid supply cavity from bottom to top.

[0009] Further, the first etching unit is used to form a rough structure on the surface of the base layer.

[0010] Furthermore, the coating unit is used to sequentially form a first infection-inhibiting layer, a second infection-inhibiting layer, and a third infection-inhibiting layer on the outer wall of the substrate.

[0011] Furthermore, the production equipment for the infection-inhibiting indwelling needle also includes a second etching unit for forming a micron-scale groove array on the outer wall of the third infection-inhibiting layer.

[0012] Furthermore, the equipment for suppressing infection with indwelling needles also includes a reciprocating motor for driving the brush mounting ring to reciprocate.

[0013] Secondly, the present invention also provides a method for producing an indwelling needle for inhibiting infection, using the aforementioned equipment for producing an indwelling needle for inhibiting infection, and the method includes the following steps:

[0014] Step 1: Provide a base tube blank, turn on the first etching unit, and perform plasma etching on the outer wall of the base tube blank to form a rough structure and obtain the base layer;

[0015] Step 2: Place the circular brush on the outer wall of the base layer and drive the circular brush to slide back and forth relative to the base layer. The organic solvent solution of the first anti-infection layer material in the liquid supply chamber flows to the circular brush through the liquid pipeline and the brush installation circle, coating all the organic solvent solution of the first anti-infection layer material onto the base layer. The circular brush then stops sliding.

[0016] Step 3: Curing the first infection-inhibiting layer material to obtain the first infection-inhibiting layer. The contact surface between the first infection-inhibiting layer and the base layer has a rough structure.

[0017] Step 4: Drive the circular brush to slide back and forth relative to the first anti-infection layer. The organic solvent solution of the second anti-infection layer material in the liquid supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, coating all the organic solvent solution of the second anti-infection layer material onto the first anti-infection layer. The circular brush then stops sliding.

[0018] Step 5: Curing the second infection-inhibiting layer material to obtain the second infection-inhibiting layer;

[0019] Step 6: Drive the circular brush to slide back and forth relative to the second anti-infection layer. The organic solvent solution of the third anti-infection layer material in the liquid supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, coating all the organic solvent solution of the third anti-infection layer material onto the second anti-infection layer. The circular brush then stops sliding.

[0020] Step 7: Curing the third infection-inhibiting layer material to obtain the third infection-inhibiting layer;

[0021] Step 8: Place the indwelling catheter over the outer wall of the puncture needle to obtain an indwelling needle that inhibits infection.

[0022] Furthermore, the following steps are included between steps 7 and 8:

[0023] Plasma etching technology was used to etch the outer wall of the third infection-inhibiting layer to form a micron-scale groove array.

[0024] Furthermore, the width of the grooves on the surface of the third infection-inhibiting layer is 5–10 μm.

[0025] Furthermore, the depth of the grooves on the surface of the third infection-inhibiting layer is 2–3 μm.

[0026] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects:

[0027] A) The equipment for producing infection-inhibiting indwelling needles provided by this invention has a simple structure and can realize the production of infection-inhibiting indwelling needles with multi-layer composite structures.

[0028] B) The production equipment for the indwelling needle for inhibiting infection provided by the present invention, due to the different materials of the base layer and the first inhibitory layer, can form a rough structure on the outer wall of the base layer by setting the first etching unit, thereby making the contact surface between the base layer and the first inhibitory layer a rough structure. The rough structure can ensure the connection stability between the base layer and the first inhibitory layer, reduce the situation where the first inhibitory layer, the second inhibitory layer and the third inhibitory layer fall off the base layer, and ensure sufficient and long-lasting anti-infection efficacy of the indwelling tube.

[0029] C) The production equipment for the indwelling needle for inhibiting infection provided by the present invention includes, in the fluid supply chamber, organic solvent solutions of the first, second, and third infection-inhibiting layers are sequentially arranged from bottom to top. Then, the organic solvent solution of the pyridine thionone compound material in the fluid supply chamber is sequentially coated onto the base layer by a circular brush, achieving one-time feeding and coating of multiple infection-inhibiting layers. In the first, second, and third infection-inhibiting layers, the pyridine thionone compound molecules are effectively bonded to the elastic base layer molecular chains through supramolecular forces (hydrogen bonds), making the pyridine thionone compound and the elastic base layer tightly combined. This effectively enhances the overall performance of the first, second, and third infection-inhibiting layers. At the same time, pyridine thionone compounds have the characteristics of low toxicity and high efficiency. Coating with this type of material can inhibit the growth of various bacteria, achieve long-term infection inhibition, and reduce the risk of infection.

[0030] In this invention, the above-described technical solutions can be combined with each other to achieve more preferred combinations. Other features and advantages of this invention will be set forth in the following description, and some advantages may become apparent from the description or be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained through the embodiments described and the accompanying drawings, which are particularly pointed out. Attached Figure Description

[0031] The accompanying drawings are for illustrative purposes only and are not intended to limit the invention. Throughout the drawings, the same reference numerals denote the same parts.

[0032] Figure 1 A schematic diagram of the production equipment for the infection-inhibiting indwelling needle provided by the present invention;

[0033] Figure 2 This is a schematic diagram of the structure of an infection-inhibiting indwelling needle produced by the production equipment for the infection-inhibiting indwelling needle provided by the present invention.

[0034] Figure label:

[0035] 1-Base layer; 2-First infection-inhibiting layer; 3-Second infection-inhibiting layer; 4-Third infection-inhibiting layer; 5-Circular brush; 6-Brush mounting ring; 7-Liquid pipeline; 8-Liquid supply chamber; 9-Puncture needle. Detailed Implementation

[0036] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which constitute a part of the present invention and are used together with the embodiments of the present invention to illustrate the principles of the present invention, but are not intended to limit the scope of the present invention.

[0037] In a first aspect, the present invention provides a production apparatus for an indwelling needle that inhibits infection, see [link to relevant documentation]. Figure 1 The system includes a first etching unit and a coating unit arranged sequentially. The coating unit includes a circular brush 5, a brush mounting ring 6, a liquid pipeline 7, and a liquid supply chamber 8. The brush mounting ring 6 has a hollow structure. The liquid outlet of the liquid supply chamber 8 is connected to the liquid inlet of the liquid pipeline 7. The liquid outlet of the liquid pipeline 7 is connected to the liquid inlet of the brush mounting ring 6. The circular brush 5 is disposed on the inner wall of the brush mounting ring 6 and contacts the liquid outlet of the brush mounting ring 6.

[0038] It should be noted that for the structure of indwelling needles used to suppress infection, please refer to [link to documentation]. Figure 2The device includes a puncture needle 9 and an indwelling tube sleeved on the outer wall of the puncture needle 9. The indwelling tube includes a base layer 1 and a first infection-inhibiting layer 2, a second infection-inhibiting layer 3, and a third infection-inhibiting layer 4 sequentially formed on the outer wall of the base layer 1. The contact surface between the base layer 1 and the first infection-inhibiting layer 2 has a rough structure. The materials of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4 include an elastic base layer (e.g., polyurethane elastomer) and a pyridine thione compound, with a mass ratio of 100:5 to 8. The pyridine thione compound molecules are hydrogen-bonded to the molecular chains of the elastic base layer.

[0039] Accordingly, the first etching unit is used to form a rough structure on the surface of the substrate 1, and the coating unit is used to sequentially form a first infection-inhibiting layer 2, a second infection-inhibiting layer 3, and a third infection-inhibiting layer 4 on the outer wall of the substrate 1. The liquid supply chamber 8 is provided with, from bottom to top, an organic solvent solution of the first infection-inhibiting layer 2 material, an organic solvent solution of the second infection-inhibiting layer 3 material, and an organic solvent solution of the third infection-inhibiting layer 4 material.

[0040] It should be noted that layering can be achieved by introducing branches of different densities into the polyurethane elastomer molecular chain, or by using organic solvents of different densities.

[0041] Compared with the prior art, the production equipment for the infection-inhibiting indwelling needle provided by the present invention has a simple structure and can realize the production of infection-inhibiting indwelling needles with multi-layer composite structures.

[0042] On the one hand, since the base layer 1 and the first infection-inhibiting layer 2 are made of different materials, the first etching unit can form a rough structure on the outer wall of the base layer 1, thereby making the contact surface between the base layer 1 and the first infection-inhibiting layer 2 a rough structure. The rough structure can ensure the connection stability between the base layer 1 and the first infection-inhibiting layer 2, reduce the possibility of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3 and the third infection-inhibiting layer 4 falling off the base layer 1, and ensure sufficient and long-lasting anti-infection efficacy of the indwelling tube.

[0043] On the other hand, in the supply chamber 8, the organic solvent solutions of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4 are sequentially arranged from bottom to top. Then, the organic solvent solution of the pyridine thionone compound material in the supply chamber 8 is sequentially coated onto the base layer 1 through the circular brush 5, realizing the coating of multiple infection-inhibiting layers (i.e., the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4) with one feeding. In the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4, the pyridine thionone compound molecules are effectively bonded to the elastic base layer molecular chains through supramolecular forces (hydrogen bonds), so that the pyridine thionone compound and the elastic base layer are tightly combined, which can effectively enhance the overall performance of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4. At the same time, the pyridine thionone compound has the characteristics of low toxicity and high efficiency. Coating with this type of material can inhibit the growth of various bacteria, achieve long-term infection inhibition, and reduce the risk of infection.

[0044] To reduce the area for bacterial biofilm formation, the manufacturing equipment for the aforementioned infection-inhibiting indwelling needle also includes a second etching unit for forming a micron-scale groove array on the outer wall of the third infection-inhibiting layer 4. This micron-scale groove array, through a triple mechanism of "physical isolation - mechanical disruption - liquid removal," inhibits biofilm formation. The regular geometric structure of the grooves causes the surface of the third infection-inhibiting layer 4 to exhibit periodic undulations, significantly reducing the actual contact area between bacteria and the third infection-inhibiting layer 4. Bacteria can only adhere through a few contact points at the groove edges, making stable adhesion difficult. Simultaneously, the sharp edges of the grooves create localized stress concentration on the bacterial cell membrane, leading to membrane deformation or even rupture, thereby further improving the long-term infection-inhibiting effect of the aforementioned infection-inhibiting indwelling needle.

[0045] It is understandable that, in order to enable the circular brush 5 and the brush mounting ring 6 to slide back and forth axially relative to the base layer 1, the aforementioned production equipment for the infection-inhibiting indwelling needle also includes a reciprocating motor for driving the reciprocating motion of the brush mounting ring 6. By driving the circular brush 5 and the brush mounting ring 6 to slide back and forth axially relative to the base layer 1 through the reciprocating motor, the organic solvent solution of the first infection-inhibiting layer 2 material, the organic solvent solution of the second infection-inhibiting layer 3 material, and the organic solvent solution of the third infection-inhibiting layer 4 material are coated.

[0046] To facilitate the transition between the base layer 1 and the first, second, and third infection-inhibiting layers 2, 3, and 4, the content of pyridine thiophene compounds in the organic solvents of the first, second, and third infection-inhibiting layers 2 and 4 gradually increases. This adjustment of the pyridine thiophene compound content ensures that the elastic base layer content in the first infection-inhibiting layer 2 is closer to that of the base layer 1, improving the bonding performance between the first infection-inhibiting layer 2 and the base layer 1 and preventing the first infection-inhibiting layer 2 from detaching from the base layer 1. Simultaneously, the outermost third infection-inhibiting layer 4 has the highest content of pyridine thiophene compounds, thus effectively reducing the amount of pyridine thiophene compounds added while maintaining the infection-inhibiting effect.

[0047] For example, the ratio of the content of pyridinethione compounds in the first infection-inhibiting layer 2, the content of pyridinethione compounds in the second infection-inhibiting layer 3, and the content of pyridinethione compounds in the third infection-inhibiting layer 4 is 1-3:4-6:7-10.

[0048] Secondly, the present invention provides a method for producing an indwelling needle for inhibiting infection, using the production equipment for the indwelling needle for inhibiting infection provided in the first aspect, the method comprising the following steps:

[0049] Step 1: Provide a base tube blank, turn on the first etching unit, and perform plasma etching on the outer wall of the base tube blank to form a rough structure, thus obtaining base layer 1;

[0050] Step 2: Place the circular brush 5 on the outer wall of the base layer 1, drive the circular brush 5 to slide back and forth relative to the base layer 1, and the organic solvent solution of the first infection-inhibiting layer 2 material in the liquid supply chamber 8 flows to the circular brush 5 through the liquid pipeline 7 and the brush mounting ring 6 in sequence, so that the organic solvent solution of the first infection-inhibiting layer 2 material is completely coated on the base layer 1, and the circular brush 5 stops sliding.

[0051] Step 3: Curing the first infection-inhibiting layer 2 material to obtain the first infection-inhibiting layer 2. The contact surface between the first infection-inhibiting layer 2 and the base layer 1 has a rough structure.

[0052] Step 4: Drive the circular brush 5 to slide back and forth relative to the first infection-inhibiting layer 2. The organic solvent solution of the second infection-inhibiting layer 3 material in the liquid supply chamber 8 flows to the circular brush 5 through the liquid pipeline 7 and the brush mounting ring 6 in sequence, so that the organic solvent solution of the second infection-inhibiting layer 3 material is completely coated on the first infection-inhibiting layer 2, and the circular brush 5 stops sliding.

[0053] Step 5: Curing the second infection-inhibiting layer 3 material to obtain the second infection-inhibiting layer 3;

[0054] Step 6: Drive the circular brush 5 to slide back and forth relative to the second infection inhibition layer 4. The organic solvent solution of the third infection inhibition layer 4 material in the liquid supply chamber 8 flows to the circular brush 5 through the liquid pipeline 7 and the brush mounting ring 6 in sequence, coating all the organic solvent solution of the third infection inhibition layer 4 material onto the second infection inhibition layer 3. The circular brush 5 then stops sliding.

[0055] Step 7: Curing the third infection-inhibiting layer 4 material to obtain the third infection-inhibiting layer 4;

[0056] Step 8: Place the indwelling catheter onto the outer wall of the puncture needle 9 to obtain an indwelling needle that inhibits infection.

[0057] It should be noted that the specific curing process parameters in steps 3, 5, and 7 above can be selected according to the actual curing conditions and the different organic solvents.

[0058] Compared with the prior art, the beneficial effects of the method for producing an indwelling needle for inhibiting infection provided by the present invention are basically the same as the beneficial effects of the production equipment for producing an indwelling needle for inhibiting infection provided in the first aspect, and will not be elaborated here.

[0059] In order to form a micrometer-scale groove array, the following steps are included between steps 7 and 8 above:

[0060] The second etching unit is activated, and plasma etching technology is used to etch the outer wall of the third infection suppression layer 4 to form a micron-scale groove array.

[0061] For example, the width of the groove on the surface of the third infection-inhibiting layer 4 is 5 to 10 μm, and the depth of the groove is 2 to 3 μm.

[0062] For example, the following steps are included before step 1 above:

[0063] Organic solvent solutions of materials for the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4 are prepared.

[0064] Specifically, the preparation methods of the organic solvent solutions for the materials of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4 are basically the same, all including the following steps:

[0065] Step A: Stir the dried hydroxyl-terminated polymer and polyisocyanate, and react them at 75-82°C for 1.5-2.5 h in a protective atmosphere to obtain a polyurethane prepolymer. The hydroxyl-terminated polymer is one or more of polymethyl dimethylsiloxane oxide (PDMS), polytetrahydrofuran ether diol (PTMEG), and polycaprolactone diol (PCL) in any proportion, with a molecular weight of 1000-4000 g / mol. The polyisocyanate is one or more of toluene diisocyanate (TDI), diphenylmethane diisocyanate (MDI), and dicyclohexylmethane diisocyanate (HMDI) in any proportion.

[0066] Step B: Add the organic solvent solution of the chain extender to the polyurethane prepolymer and react at 75-82°C for 4-5 hours. Mix and stir to obtain an organic solvent solution of polyurethane elastomer. The chain extender is one or more of 1,4-butanediol, ethylene glycol, and diaminopyridine in any proportion.

[0067] Step C: Add the organic solvent solution of pyridinethione compound to the organic solvent solution of polyurethane elastomer, and react at 75-82°C for 5-10 hours to obtain organic solvent solutions of the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4, respectively. The pyridinethione compound is one or more of pyridinethione, sodium pyridinethione, and zinc pyridinethione in any proportion.

[0068] It should be noted that the preparation process of the organic solvent solution for the first infection-inhibiting layer 2, the second infection-inhibiting layer 3, and the third infection-inhibiting layer 4 is the same, the only difference being the ratio of pyridine thionone compounds to polyurethane elastomer.

[0069] Existing technologies mainly focus on adding reactive pyridine thionone compounds during the synthesis stage of polyurethane elastomers and on melt blending polyurethane elastomers with pyridine thionone compounds. The production method of the organic solvent solution of the above-mentioned anti-infection layer material adopts the solution blending method, in which pyridine thionone compounds are added to polyurethane elastomers and dispersed at the molecular level, so that pyridine thionone compound molecules and polyurethane elastomer molecular chains form hydrogen bonds extensively, thereby achieving a long-term anti-infection effect.

[0070] For example, in the above preparation method, the organic solvent solution raw materials of the polyurethane elastomer include 60-84% hydroxyl-terminated polymer, 13-33% polyisocyanate, 3-7% chain extender and 0.3-0.6% organic solvent, and the organic solvent solution of the pyridine thionone compound includes 2-8% pyridine thionone compound and 92-98% organic solvent, calculated by mass percentage.

[0071] The present invention provides embodiments 1 to 3, which specifically describe the production method of the indwelling needle for inhibiting infection, comprising the following steps:

[0072] Step a: Provide a puncture needle and a base tube blank;

[0073] Step b: Stir the dried hydroxyl-terminated polymer and polyisocyanate and react them under a protective atmosphere to obtain a polyurethane prepolymer;

[0074] Step c: Add the organic solvent solution of the chain extender to the polyurethane prepolymer, react, mix and stir to obtain the organic solvent solution of the polyurethane elastomer;

[0075] Step d: Add the organic solvent solution of pyridine thionone compound to the organic solvent solution of polyurethane elastomer to react and obtain the organic solvent solutions of the first anti-infection layer, the second anti-infection layer and the third anti-infection layer materials, respectively.

[0076] Step e: Sequentially add the organic solvent solution of the first, second, and third infection-inhibiting layer materials into the liquid supply chamber;

[0077] Step f: Etch the outer wall of the base tube blank to form a rough structure and obtain the base layer;

[0078] Step g: Place the circular brush onto the outer wall of the base layer;

[0079] Step h: Drive the circular brush to slide back and forth relative to the base layer. The organic solvent solution of the first anti-infection layer material in the liquid supply chamber flows to the circular brush through the liquid pipeline and the brush installation circle, coating all the organic solvent solution of the first anti-infection layer material onto the base layer. The circular brush then stops sliding.

[0080] Step i: Curing the first infection-inhibiting layer material to obtain the first infection-inhibiting layer;

[0081] Step j: Drive the circular brush to slide back and forth relative to the first anti-infection layer. The organic solvent solution of the second anti-infection layer material in the liquid supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, coating all the organic solvent solution of the second anti-infection layer material onto the first anti-infection layer. The circular brush then stops sliding.

[0082] Step k: Curing the second infection-inhibiting layer material to obtain the second infection-inhibiting layer;

[0083] Step 1: Drive the circular brush to slide back and forth relative to the second anti-infection layer. The organic solvent solution of the third anti-infection layer material in the liquid supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, coating all the organic solvent solution of the third anti-infection layer material onto the second anti-infection layer. The circular brush then stops sliding.

[0084] Step m: Curing the third infection-inhibiting layer material to obtain the third infection-inhibiting layer;

[0085] Step n: Place the indwelling catheter on the outer wall of the puncture needle.

[0086] For the specific process parameters in the production methods of the indwelling needles for inhibiting infection in Examples 1 to 3, please refer to Table 1.

[0087]

[0088] The indwelling needles for inhibiting infection prepared in Examples 1-3 were tested for their anti-infection activity using the surface contact method: Bacterial suspensions (Escherichia coli or Staphylococcus aureus) were cultured in Mueller-Hinton Broth (MHB) medium at 37°C until the optical density at 600 nm reached 0.8, indicating the entry into the logarithmic growth phase. The bacterial cells were collected at 4°C, washed with sterile PBS (pH 7.4), and resuspended in PBS to a final concentration of 6 × 10⁻⁶. 6 cells / mL. Examples 1-3 and Comparative Example 1 (area 0.01-0.04 cm²) were compared. 2 Add 50 μL of bacterial suspension and shake at 37 °C for 4 h. Then, take 25 μL of the suspension and inoculate it onto sterile LB culture dishes at a series of gradient concentrations (×1, ×10, ×100, ×1000).

[0089] After incubation at 37°C for 12 hours, bacterial colonies were counted, and Examples 1-3 were compared with Comparative Example 1. The inhibition rates of Examples 1-3 and Comparative Example 1 against Escherichia coli and Staphylococcus aureus were all 100%.

[0090] Examples 1-3 and Comparative Example 1 were each added to 50 μL of bacterial suspension and shaken for 15 days. After shaking, the suspension was removed, rinsed with water, and then added back to 50 μL of freshly prepared bacterial suspension (the bacterial suspension production method is as described above). The suspension was shaken at 37°C for 4 hours. Then, 25 μL of the suspension was inoculated onto sterile LB petri dishes at a series of gradient concentrations (×1, ×10, ×100, ×1000). After incubation at 37°C for 12 hours, bacterial colony counts were performed. Examples 1-3 showed that the inhibition rate against Escherichia coli and Staphylococcus aureus remained above 76%.

[0091] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for producing an indwelling needle for inhibiting infection, characterized in that, The indwelling needle for inhibiting infection includes a puncture needle and an indwelling tube sleeved on the outer wall of the puncture needle. The indwelling tube includes a base layer and a first, second, and third infection-inhibiting layer sequentially formed on the outer wall of the base layer. The production method uses production equipment, which includes a first etching unit and a coating unit arranged sequentially. The coating unit includes a circular brush, a brush mounting ring, a liquid pipeline, and a liquid supply chamber. The brush mounting ring has a hollow structure. The outlet end of the liquid supply chamber is connected to the inlet end of the liquid pipeline, and the outlet end of the liquid pipeline is connected to the brush mounting ring. The brush mounting ring is connected to the liquid inlet, and the circular brush is disposed on the inner wall of the brush mounting ring and in contact with the liquid outlet of the brush mounting ring; the production method includes the following steps: Step 1: Provide a base tube blank, turn on the first etching unit, and perform plasma etching on the outer wall of the base tube blank to form a rough structure and obtain the base layer; Step 2: Place the circular brush on the outer wall of the base layer, drive the circular brush to slide back and forth relative to the base layer, and the organic solvent solution of the first anti-infection layer material in the supply chamber flows to the circular brush through the liquid pipeline and the brush mounting ring in sequence, so as to apply the first anti-infection layer... Step 3: The organic solvent solution of the material is completely coated on the base layer, and the circular brush stops sliding; Step 4: The first anti-infection layer material is cured to obtain the first anti-infection layer, and the contact surface between the first anti-infection layer and the base layer has a rough structure; Step 5: The circular brush is driven to slide back and forth relative to the first anti-infection layer, and the organic solvent solution of the second anti-infection layer material in the supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, so that the organic solvent solution of the second anti-infection layer material is completely coated on the first anti-infection layer, and the circular brush stops sliding; Step 6: The organic solvent solution of the second anti-infection layer material is completely coated on the first anti-infection layer, and the circular brush stops sliding; Step 6: The second infection-inhibiting layer material is cured to obtain the second infection-inhibiting layer; Step 7: The circular brush is driven to slide back and forth relative to the second infection-inhibiting layer, and the organic solvent solution of the third infection-inhibiting layer material in the supply chamber flows sequentially through the liquid pipeline and the brush installation to the circular brush, so that the organic solvent solution of the third infection-inhibiting layer material is completely coated on the second infection-inhibiting layer, and the circular brush stops sliding; Step 8: The third infection-inhibiting layer material is cured to obtain the third infection-inhibiting layer; Step 9: The indwelling tube is sleeved on the outer wall of the puncture needle to obtain the infection-inhibiting indwelling needle.

2. The method for producing the indwelling needle for inhibiting infection according to claim 1, characterized in that, The liquid supply chamber contains, from bottom to top, organic solvent solutions of the first infection-inhibiting layer material, the second infection-inhibiting layer material, and the third infection-inhibiting layer material.

3. The method for producing the indwelling needle for inhibiting infection according to claim 1, characterized in that, The first etching unit is used to form a rough structure on the substrate surface.

4. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The coating unit is used to sequentially form a first infection-inhibiting layer, a second infection-inhibiting layer, and a third infection-inhibiting layer on the outer wall of the base layer.

5. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The production equipment for the infection-inhibiting indwelling needle also includes a second etching unit for forming a micron-scale groove array on the outer wall of the third infection-inhibiting layer.

6. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The production equipment for the infection-inhibiting indwelling needle also includes a reciprocating motor for driving the brush mounting ring to reciprocate.

7. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The following steps are also included between step 7 and step 8: Plasma etching technology was used to etch the outer wall of the third infection-inhibiting layer to form a micron-scale groove array.

8. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The width of the grooves on the surface of the third infection-inhibiting layer is 5~10μm.

9. The method for producing an indwelling needle for inhibiting infection according to claim 1, characterized in that, The depth of the grooves on the surface of the third infection-inhibiting layer is 2~3μm.

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