Airlift biological reaction device for culturing tobacco hairy roots

Abstract

The invention discloses an airlift biological reaction device for culturing tobacco hairy roots, and belongs to the technical field of tobacco hairy root cultivation, the device comprises a reaction cylinder, the top of the reaction cylinder is provided with a stirring assembly, the interior of the reaction cylinder is provided with an inflation assembly, and the bottom of the reaction cylinder is provided with a dilution assembly; the angles of the stirring blades are driven to be adjusted through components such as springs and adjusting blocks, so that self-adaptive stirring is realized; when the viscosity of the solution is too large, the resistance of the stirring blades is increased, the adjusting rod rotates to start the dilution assembly, and sterile water is introduced into the diluted solution through a water inlet pipe and other channels; the gas charging assembly can change the relative position of the baffle and the communicating groove according to the concentration of the solution, and the gas charging pressure is automatically adjusted, so that the problems of non-uniform gas distribution, difficult stirring and the like caused by the change of the viscosity of the solution in the prior art are effectively solved; good stirring, gas supply and solution concentration adjustment in each reaction stage in the tobacco hairy root cultivation process are ensured, and the hairy root growth and secondary metabolite synthesis effects are improved.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco hairy root cultivation, in particular to an airlift bioreactor for cultivating tobacco hairy roots. Background Art

[0002] Tobacco hairy root cultivation typically involves explant disinfection, infection with Agrobacterium rhizogenes, co-cultivation, sterilization, and subsequent subculture. First, the tobacco explants are rigorously disinfected to eliminate surface microorganisms. The disinfected explants are then infected with Agrobacterium rhizogenes, prompting the integration of the T-DNA on the Agrobacterium Ri plasmid into the tobacco cell genome. After co-cultivation to allow the Agrobacterium to fully interact with the tobacco cells, sterilization is performed to remove any remaining Agrobacterium. Finally, the successfully induced hairy roots are transferred to a suitable culture medium for subculture to achieve substantial proliferation and yield the desired secondary metabolites.

[0003] The viscosity of the reaction solution changes significantly throughout the cultivation process. Initially, the viscosity is primarily determined by the composition of the culture medium. As the hairy roots grow, their metabolic products are continuously secreted, and substances such as polysaccharides and proteins gradually accumulate, causing the viscosity to rise continuously. After entering the stable phase, the viscosity stabilizes but remains high.

[0004] However, this change in viscosity brings many problems to the cultivation process. When the viscosity is too high, it makes it difficult for gas to penetrate the solution, resulting in uneven distribution of gas in the solution and insufficient oxygen supply. The hairy roots cannot obtain sufficient oxygen for respiration and metabolism. High viscosity also increases the difficulty of stirring, increases stirring resistance, reduces stirring efficiency, and makes it difficult to ensure uniform mixing of the reaction solution, which in turn affects the transfer of nutrients and the growth environment of the hairy roots, ultimately leading to slow growth of the hairy roots and reduced synthesis of secondary metabolites.

[0005] To this end, the present invention provides an airlift bioreactor for cultivating tobacco hairy roots to solve the above problems. Summary of the Invention

[0006] In view of the deficiencies in the prior art, the present invention provides an airlift bioreactor for cultivating tobacco hairy roots, which solves the above problems.

[0007] To achieve the above object, the present invention is realized through the following technical solutions: An air-lift bioreactor for cultivating tobacco hairy roots, comprising a reaction cylinder, a stirring assembly is arranged at the top of the reaction cylinder, an air-inflating assembly is arranged inside the stirring assembly, and a dilution assembly is arranged at the bottom of the stirring assembly. The stirring assembly includes a rotating cylinder, a top cylinder is fixedly installed at the top of the rotating cylinder, the top cylinder is rotatably connected inside the top wall of the reaction cylinder, a support plate is fixedly installed inside the top cylinder, an adjusting rod is rotatably connected inside the support plate, the adjusting rod is located inside the rotating cylinder, an adjusting block is fixedly installed on the outer side of the adjusting rod, a moving rod is fixedly installed on the outer side of the adjusting block, a pushing block is fixedly installed on the inner wall of the top cylinder, and a spring is fixedly installed between the corresponding moving rod and the pushing block. A rotating rod is rotatably connected to the side wall of the rotating cylinder, stirring blades are fixedly installed on the outer side of the rotating rod, a first bevel gear is fixedly installed at the inner end of the rotating rod, a second bevel gear is fixedly installed on the outer side of the adjusting rod, and the first bevel gear meshes with the second bevel gear.

[0008] Preferably: A bracket is fixedly installed at the top of the reaction cylinder, a motor is fixedly installed at the top of the bracket, the output end of the motor movably penetrates through the inside of the bracket, a power plate is fixedly installed at the bottom of the output end of the motor, a connecting rod is fixedly installed at the bottom of the power plate, a power cylinder is fixedly installed at the bottom of the connecting rod, and a dial rod is fixedly installed at the bottom of the power cylinder, and the dial rod is attached to the side surface of the corresponding moving rod.

[0009] Preferably: A vertical rod is fixedly installed at the bottom of the bracket, a support ring is fixedly installed at the bottom of the vertical rod, the support ring is rotatably connected to the outside of the power plate and the inside of the power cylinder, and a water inlet pipe is fixedly installed at the top of the support ring, and the water inlet pipe is communicated with the inside of the power cylinder.

[0010] Preferably: The top of the adjusting rod is rotatably connected to the bottom of the power cylinder, and an intermediate groove is opened inside the adjusting rod, and the intermediate groove is communicated with the inside of the power cylinder.

[0011] Preferably: A bottom cylinder is fixedly installed at the bottom of the rotating cylinder, the bottom of the adjusting rod movably penetrates through the bottom wall of the rotating cylinder and the top wall of the bottom cylinder, and an inner cylinder is fixedly installed at the bottom of the adjusting rod, and the inner cylinder is communicated with the inside of the intermediate groove.

[0012] Preferably: An arc-shaped baffle is fixedly installed on the inner bottom wall of the bottom cylinder, a discharge groove is opened on the side wall of the inner cylinder, the arc-shaped baffle is attached to the outside of the inner cylinder, and the corresponding arc-shaped baffle is attached to the outside of the corresponding discharge groove. A second one-way nozzle is fixedly installed on the side wall of the bottom cylinder, and the communication direction of the second one-way nozzle is from the inside of the bottom cylinder to the outside.

[0013] Preferably, the inflation assembly includes a fixing ring. A support rod is fixedly installed between the fixing ring and the top of the reaction cylinder. An air inlet pipe is fixedly installed on the side of the fixing ring, and the air inlet pipe communicates with the inside of the fixing ring. The fixing ring is rotatably connected between the power cylinder and the top cylinder. A communication groove is formed inside the support plate, and the communication groove communicates the upper and lower parts above the top cylinder. The lower part of the top cylinder communicates with the inside of the rotating cylinder.

[0014] Preferably, a first one-way nozzle is fixedly installed on the outer side of the rotating cylinder, and the communication direction of the first one-way nozzle is from the inside of the rotating cylinder to the outside. A baffle is fixedly installed at the bottom of the moving rod, and the baffle fits above the communication groove.

[0015] Preferably, a feeding port and a pneumatic balance valve are fixedly installed on the top of the reaction cylinder, and a discharge valve is fixedly installed at the bottom of the reaction cylinder.

[0016] Beneficial effects

[0017] The present invention provides an airlift bioreactor for culturing tobacco hairy roots. Compared with the prior art, it has the following beneficial effects:

[0018] 1. The airlift bioreactor for culturing tobacco hairy roots realizes adaptive stirring of solutions with different concentrations through the stirring assembly. When the solution concentration changes, the resistance received by the stirring blades changes, pushing the spring to contract to different degrees, and then driving the adjusting block and the adjusting rod to rotate, realizing the adjustment of the angle of the stirring blades. For solutions with low viscosity, the stirring blades at a smaller angle can generate stronger axial flow, improving the mixing efficiency and avoiding shear damage to the tobacco hairy roots at the same time. For solutions with high viscosity, the stirring blades at a larger angle increase the radial pushing effect, reducing solution wall sticking and accumulation, and ensuring good stirring effects in each reaction stage.

[0019] 2. When the viscosity of the solution in the reaction cylinder is too high and affects the reaction, the resistance received by the stirring blades increases, and the relative rotation angle between the adjusting rod and the rotating cylinder reaches the maximum, causing the inner cylinder and the bottom cylinder to rotate relative to each other, and the arc-shaped baffle no longer seals the discharge groove. The sterile water passes through the power cylinder, the intermediate groove, and the inner cylinder from the water inlet pipe and is introduced into the solution through the second one-way nozzle for dilution, and automatically stops after the concentration drops, ensuring the normal progress of the reaction.

[0020] 3. The inflation assembly of the airlift bioreactor for culturing tobacco hairy roots can automatically adjust the gas inlet pressure according to the solution concentration. As the solution concentration changes, the relative position between the rotating cylinder and the adjusting rod changes, and the position of the baffle covering the communication groove changes accordingly, thereby adjusting the amount of gas entering the rotating cylinder per unit time. When the solution concentration is low, the gas is evenly dispersed; when the concentration is high, the gas pressure is increased to ensure that the gas fully enters the system and maintain the smooth progress of the reaction. Brief Description of the Drawings

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0022] Figure 1 is a three-dimensional external structure diagram of the present invention;

[0023] Figure 2 is a three-dimensional internal structure diagram of the present invention;

[0024] Figure 3 is a three-dimensional internal overall structure diagram of the present invention;

[0025] Figure 4 is of the present invention Figure 3 enlarged structure diagram of part A;

[0026] Figure 5 is a three-dimensional external structure diagram of the stirring assembly and the dilution assembly of the present invention

[0027] Figure 6 is a three-dimensional internal sectional structure diagram of the stirring assembly of the present invention;

[0028] Figure 7 is a three-dimensional internal sectional structure diagram of the dilution assembly of the present invention;

[0029] Figure 8 is a three-dimensional internal structure diagram of the stirring assembly of the present invention;

[0030] Figure 9 is a three-dimensional exploded internal structure diagram of the stirring assembly of the present invention;

[0031] Figure 10 is a three-dimensional internal structure diagram of the dilution assembly of the present invention.

[0032] In the figure: 1, reaction cylinder; 2, stirring assembly; 21, rotating cylinder; 22, stirring blades; 23, rotating rod; 24, top cylinder; 25, power cylinder; 26, shifting rod; 27, power plate; 28, connecting rod; 29, adjusting rod; 210, first bevel gear; 211, second bevel gear; 212, support plate; 213, adjusting block; 214, moving rod; 215, spring; 216, pushing block; 3, air inflation assembly; 31, first one-way nozzle; 32, fixing ring; 33, support rod; 34, air inlet pipe; 35, baffle plate; 36, communicating groove; 4, dilution assembly; 41, bottom cylinder; 42, second one-way nozzle; 43, inner cylinder; 44, middle groove; 45, discharge groove; 46, arc-shaped baffle plate; 47, support ring; 48, water inlet pipe; 49, vertical rod; 5, feeding port; 6, air pressure balance valve; 7, discharge valve; 8, support; 9, motor. Detailed implementation manners

[0033] It should be noted that in the description of the embodiments of the present application, the orientation or positional relationships indicated by terms such as "front, back", "left, right", "up, down", etc. are all based on the orientation or positional relationships shown in the drawings. It is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application. The terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific situations.

[0034] The present application will be further described in detail below with reference to the drawings and embodiments.

[0035] Refer to Figures 1 to 10, an embodiment of the present application provides an air-lift bioreactor for cultivating tobacco hairy roots, including a reaction cylinder 1. A stirring assembly 2 is arranged at the top of the reaction cylinder 1. An air inflation assembly 3 is arranged inside the stirring assembly 2. A dilution assembly 4 is arranged at the bottom of the stirring assembly 2. The stirring assembly 2 includes a rotating cylinder 21. A top cylinder 24 is fixedly installed at the top of the rotating cylinder 21. The top cylinder 24 is rotatably connected inside the top wall of the reaction cylinder 1. A support plate 212 is fixedly installed inside the top cylinder 24. An adjusting rod 29 is rotatably connected inside the support plate 212. The adjusting rod 29 is located inside the rotating cylinder 21. An adjusting block 213 is fixedly installed on the outer side of the adjusting rod 29. A moving rod 214 is fixedly installed on the outer side of the adjusting block 213. A pushing block 216 is fixedly installed on the inner wall of the top cylinder 24. A spring 215 is fixedly installed between the corresponding moving rod 214 and the pushing block 216. A rotating rod 23 is rotatably connected to the side wall of the rotating cylinder 21. Stirring blades 22 are fixedly installed on the outer side of the rotating rod 23. A first bevel gear 210 is fixedly installed at the inner end of the rotating rod 23. A second bevel gear 211 is fixedly installed on the outer side of the adjusting rod 29. The first bevel gear 210 and the second bevel gear 211 are meshed with each other.

[0036] A bracket 8 is fixedly installed at the top of the reaction cylinder 1. A motor 9 is fixedly installed at the top of the bracket 8. The output end of the motor 9 movably penetrates inside the bracket 8. A power plate 27 is fixedly installed at the bottom of the output end of the motor 9. A connecting rod 28 is fixedly installed at the bottom of the power plate 27. A power cylinder 25 is fixedly installed at the bottom of the connecting rod 28. A dial rod 26 is fixedly installed at the bottom of the power cylinder 25. The dial rod 26 is attached to the side of the corresponding moving rod 214.

[0037] In this embodiment, when culturing tobacco hairy roots, the raw material nutrient solution and the like are fed into the interior of the reaction cylinder 1 through the feeding port 5. The motor 9 is started to drive the power plate 27 to rotate, and the connecting rod 28 drives the entire power cylinder 25 to rotate. The rotation of the power cylinder 25 drives the lower dial rod 26 to rotate. The lower moving rod 214 is toggled by the dial rod 26. After the moving rod 214 is toggled, the spring 215 contracts. The force exerted by the moving rod 214 on the spring 215 acts on the push block 216, thereby pushing the push block 216 and the outer top cylinder 24 to rotate. The rotation of the top cylinder 24 drives the lower rotating cylinder 21 to rotate, and the rotation of the rotating cylinder 21 drives the stirring blades 22 to rotate, so as to stir and mix the internal reactants, with a relatively fast reaction rate. When the stirring blades 22 rotate, the higher the viscosity of the internal solution, the greater the resistance to the stirring blades 22. When the dial rod 26 toggles the moving rod 214 to swing, the greater the resistance received. Therefore, the distance by which the spring 215 is compressed after the overall rotation becomes larger, thus realizing that the greater the solution concentration, the greater the contraction distance of the spring 215, and vice versa. When the spring 215 is compressed and the moving rod 214 moves, the adjusting block 213 rotates, so that the adjusting block 213 rotates relative to the top cylinder 24 and the rotating cylinder 21. The greater the degree of compression of the spring 215, the greater the relative rotation distance of the adjusting block 213. Therefore, it can be realized that the greater the solution concentration, the greater the rotation angle of the adjusting block 213. The rotation of the adjusting block 213 can drive the adjusting rod 29 to rotate, and the rotation of the adjusting rod 29 can drive the second bevel gear 211 to rotate, and synchronously drive the first bevel gear 210 and the rotating rod 23 to rotate, so as to adjust the angle of the stirring blades 22. The initial angle of the stirring blades 22 is 45 degrees. The greater the liquid concentration, the greater the angle of the stirring blades 22, and the maximum adjustment distance is 90 degrees. For low-viscosity solutions, the angle of the stirring blades 22 is small. Such an angle can cause a strong axial flow of the solution during stirring, making the overall solution flow and improving the mixing efficiency. At the same time, it avoids excessive shear force caused by too large an angle and damaging the tobacco hairy roots that are vulnerable to shear force. For high-viscosity solutions, a larger angle can increase the radial pushing effect of the paddle on the solution, enabling the viscous solution to better turn up and down in the reactor, reducing the phenomenon of solution sticking to the wall and accumulation, improving the stirring effect, and promoting the uniform distribution of substances. Through this method, it can be ensured that a good stirring reaction effect can be achieved at different solution concentrations for each reaction time;

[0038] When the stirring blade 22 is stirring and the liquid concentration remains unchanged, the rotating cylinder 21 and the adjusting rod 29 rotate synchronously and are relatively stationary to each other. When the liquid concentration changes and the obstruction on the stirring blade 22 changes, the force required to drive the push block 216 to move by squeezing the spring 215 will change. Therefore, the degree of squeezing of the spring 215 will change. At this time, the relative positions of the adjusting block 213 and the adjusting rod 29 with respect to the rotating cylinder 21 will change. Therefore, the adjusting rod 29 rotates a certain angle relative to the rotating cylinder 21, thereby realizing the angle adjustment of the stirring blade 22.

[0039] Referring to Figures 1 to 10 , in one aspect of this embodiment, a vertical rod 49 is fixedly installed at the bottom of the bracket 8. The bottom of the vertical rod 49 is fixedly installed with a support ring 47. The support ring 47 is rotatably connected to the outside of the power plate 27 and is rotatably connected to the inside of the power cylinder 25. The top of the support ring 47 is fixedly installed with a water inlet pipe 48, and the water inlet pipe 48 communicates with the inside of the power cylinder 25. The top of the adjusting rod 29 is rotatably connected to the bottom of the power cylinder 25. An intermediate groove 44 is formed inside the adjusting rod 29, and the intermediate groove 44 communicates with the inside of the power cylinder 25. The bottom of the rotating cylinder 21 is fixedly installed with a bottom cylinder 41. The bottom of the adjusting rod 29 movably penetrates the bottom wall of the rotating cylinder 21 and the top wall of the bottom cylinder 41. The bottom of the adjusting rod 29 is fixedly installed with an inner cylinder 43, and the inner cylinder 43 communicates with the inside of the intermediate groove 44.

[0040] An arc-shaped baffle 46 is fixedly installed on the inner bottom wall of the bottom cylinder 41. A discharge groove 45 is formed on the side wall of the inner cylinder 43. The arc-shaped baffle 46 is attached to the outside of the inner cylinder 43, and the corresponding arc-shaped baffle 46 is attached to the outside of the corresponding discharge groove 45. A second one-way spray head 42 is fixedly installed on the side wall of the bottom cylinder 41, and the communication direction of the second one-way spray head 42 is from the inside to the outside of the bottom cylinder 41.

[0041] In this embodiment, the water inlet pipe 48 is connected to an external water supply pipe, and sterile water is introduced into the interior of the water inlet pipe 48. The sterile water will enter the interior of the power cylinder 25. When the power plate 27 and the power cylinder 25 rotate, it does not affect the entry of the sterile water into the interior of the power cylinder 25. After the sterile water enters the interior of the power cylinder 25, it enters the interior of the lower inner cylinder 43 through the intermediate groove 44. The discharge groove 45 is sealed by the arc-shaped baffle 46 to ensure that the sterile water does not enter the interior of the bottom cylinder 41 through the discharge groove 45. When the viscosity of the solution inside the reaction cylinder 1 is too high, it seriously affects the internal reaction effect at this time. When the stirring blades 22 rotate, a large resistance will be generated. At this time, the relative rotation angle between the adjusting rod 29 and the rotating cylinder 21 is the largest. Therefore, the relative rotation angle between the inner cylinder 43 and the bottom cylinder 41 connected to the adjusting rod 29 and the rotating cylinder 21 respectively is the largest. Therefore, the rotation angle of the arc-shaped baffle 46 relative to the inner cylinder 43 is the largest. At this time, the arc-shaped baffle 46 can no longer seal the discharge groove 45, and the sterile water inside the inner cylinder 43 will enter the interior of the bottom cylinder 41 and be introduced into the internal solution through the second one-way nozzle 42, thereby diluting the internal solution. When diluted to a certain extent, the solution concentration decreases, and the generated resistance decreases. The arc-shaped baffle 46 fits against the outside of the discharge groove 45 again, and the introduction of sterile water stops, and the internal reaction proceeds normally. Thus, when the solution concentration is too high, sterile water is automatically introduced for concentration dilution to ensure the normal progress of the reaction.

[0042] Referring to Figures 1 to 10 , in one aspect of this embodiment, the inflation assembly 3 includes a fixed ring 32. A support rod 33 is fixedly installed between the fixed ring 32 and the top of the reaction cylinder 1. An air inlet pipe 34 is fixedly installed on the side of the fixed ring 32, and the air inlet pipe 34 communicates with the interior of the fixed ring 32. The fixed ring 32 is rotatably connected between the power cylinder 25 and the top cylinder 24. A communication groove 36 is formed inside the support plate 212, and the communication groove 36 communicates the upper and lower parts above the top cylinder 24. The lower part of the top cylinder 24 communicates with the interior of the rotating cylinder 21.

[0043] A first one-way nozzle 31 is fixedly installed on the outside of the rotating cylinder 21. The communication direction of the first one-way nozzle 31 is from the interior of the rotating cylinder 21 to the outside. A baffle 35 is fixedly installed at the bottom of the moving rod 214, and the baffle 35 fits against the upper part of the communication groove 36. A feeding port 5 and a pneumatic balance valve 6 are fixedly installed on the top of the reaction cylinder 1. A discharge valve 7 is fixedly installed at the bottom of the reaction cylinder 1.

[0044] In this embodiment, the air inlet pipe 34 is connected to the external air supply pipe, and the air supply pipe transports the gas required for the reaction into the air inlet pipe 34. The gas enters the interior of the fixed ring 32 through the air inlet pipe 34, and then enters the interior of the top cylinder 24. Thereafter, the gas enters the interior of the rotating cylinder 21 through the connecting groove 36. When the gas is ejected through the first one-way nozzle 31 on the outside of the rotating cylinder 21, the reaction gas is quickly mixed with the internal solution by the rapid rotation of the first one-way nozzle 31 and the rapid stirring of the stirring blade 22. When the concentration of the internal solution changes, the relative position of the rotating cylinder 21 and the adjusting rod 29 changes, and the relative position of the baffle 35 and the support plate 212 changes. Therefore, the position of the baffle 35 covering the connecting groove 36 will change, and the solution The higher the concentration, the lower the baffle 35 covers the connecting groove 36. Therefore, the amount of gas passing through the connecting groove 36 and entering the rotating cylinder 21 per unit time becomes larger, so the pressure flow rate of the gas ejected through the first one-way nozzle 31 becomes larger. Conversely, the lower the liquid concentration, the smaller the amount of gas passing through the connecting groove 36 per unit time, and the smaller the pressure of the ejected gas. Automatically adjusting the gas introduction pressure by solution concentration can achieve the following: when the solution concentration is low, gas introduction is relatively easy, and bubbles can rise quickly, ensuring that the gas is evenly dispersed in the solution. When the solution concentration is high, the gas introduction resistance increases, the bubble rising speed slows down, and the gas dispersion becomes worse. At this time, the pressure of the gas introduction is increased to ensure that the gas can fully enter the system and maintain the reaction.

[0045] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0046] Working principle: Stirring working principle: Start the motor 9, its output end drives the power plate 27 to rotate, and the power cylinder 25 rotates through the connecting rod 28. The lever 26 at the bottom of the power cylinder 25 drives the moving rod 214, and the spring 215 contracts, pushing the top cylinder 24 to rotate, thereby driving the rotating cylinder 21 and the stirring blade 22 to rotate; when the solution concentration changes, the resistance experienced by the stirring blade 22 changes the compression degree of the spring 215, and the adjusting block 213 rotates, and the rotating rod 23 is driven to rotate through the adjusting rod 29, the second bevel gear 211, and the first bevel gear 210, thereby realizing automatic adjustment of the angle of the stirring blade 22;

[0047] Dilution working principle: The water inlet pipe 48 is connected to the external water supply pipe to introduce sterile water, which then enters the inner cylinder 43 through the power cylinder 25 and the middle tank 44. When the viscosity of the solution is too high, the obstruction of the stirring blade 22 increases, the relative rotation angle between the adjustment rod 29 and the rotating cylinder 21 reaches the maximum, the inner cylinder 43 and the bottom cylinder 41 rotate relative to each other, and the arc baffle 46 no longer seals the discharge tank 45. Sterile water enters the bottom cylinder 41 and is diluted by the second one-way nozzle 42. After the concentration drops, the arc baffle 46 reseals the discharge tank 45, and the dilution stops.

[0048] Inflation working principle: The air inlet pipe 34 is connected to an external gas supply pipeline to transport reaction gas. The gas sequentially passes through the fixed ring 32, the top cylinder 24, and the communication groove 36 and enters the rotating cylinder 21, and is ejected through the first one-way nozzle 31. When the solution concentration changes, the relative position between the rotating cylinder 21 and the adjusting rod 29 changes, and the position of the baffle 35 covering the communication groove 36 changes, so as to adjust the amount of gas entering the rotating cylinder 21 per unit time, and realize the automatic adjustment of the gas inlet pressure according to the solution concentration.

[0049] Overall working process: When cultivating tobacco hairy roots, the raw material nutrient solution and the like are sent into the reaction cylinder 1 through the feeding port 5. The motor 9 is started to drive the stirring assembly 2 to work, and at the same time, the inflation assembly 3 supplies reaction gas. During the cultivation process, the stirring assembly 2, the dilution assembly 4, and the inflation assembly 3 work together according to the change of the solution concentration to adjust the stirring effect, the solution concentration, and the gas supply in real time. After the cultivation is completed, the product is discharged through the discharge valve 7, and the air pressure balance valve 6 ensures the stable air pressure in the reaction cylinder 1.

[0050] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0051] Although the embodiments of the present application have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present application. The scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An airlift bioreactor for cultivating tobacco hairy roots, comprising a reaction cylinder (1), characterized in that: A stirring assembly (2) is provided at the top of the reaction cylinder (1). An air inflation assembly (3) is arranged inside the stirring assembly (2). A dilution assembly (4) is provided at the bottom of the stirring assembly (2). The stirring assembly (2) includes a rotating cylinder (21). A top cylinder (24) is fixedly installed at the top of the rotating cylinder (21). The top cylinder (24) is rotatably connected inside the top wall of the reaction cylinder (1). A support plate (212) is fixedly installed inside the top cylinder (24). An adjusting rod (29) is rotatably connected inside the support plate (212). The adjusting rod (29) is located inside the rotating cylinder (21). An adjusting block (213) is fixedly installed on the outer side of the adjusting rod (29). A moving rod (214) is fixedly installed on the outer side of the adjusting block (213). A pushing block (216) is fixedly installed on the inner wall of the top cylinder (24). A spring (215) is fixedly installed between the corresponding moving rod (214) and the pushing block (216). A rotating rod (23) is rotatably connected to the side wall of the rotating cylinder (21). Stirring blades (22) are fixedly installed on the outer side of the rotating rod (23). A first bevel gear (210) is fixedly installed at the inner end of the rotating rod (23). A second bevel gear (211) is fixedly installed on the outer side of the adjusting rod (29). The first bevel gear (210) and the second bevel gear (211) are meshed with each other.

2. The air-lift bioreactor for culturing tobacco hairy roots according to claim 1, wherein: A bracket (8) is fixedly installed at the top of the reaction cylinder (1). A motor (9) is fixedly installed at the top of the bracket (8). The output end of the motor (9) movably penetrates inside the bracket (8). A power plate (27) is fixedly installed at the bottom of the output end of the motor (9). A connecting rod (28) is fixedly installed at the bottom of the power plate (27). A power cylinder (25) is fixedly installed at the bottom of the connecting rod (28). A dial rod (26) is fixedly installed at the bottom of the power cylinder (25). The dial rod (26) is attached to the side surface of the corresponding moving rod (214).

3. An airlift bioreactor for culturing tobacco hairy roots according to claim 2, characterized in that: A vertical rod (49) is fixedly installed at the bottom of the bracket (8). A support ring (47) is fixedly installed at the bottom of the vertical rod (49). The support ring (47) is rotatably connected to the outside of the power plate (27). The support ring (47) is rotatably connected to the inside of the power cylinder (25). A water inlet pipe (48) is fixedly installed at the top of the support ring (47). The water inlet pipe (48) is communicated with the inside of the power cylinder (25).

4. The airlift bioreactor for cultivating tobacco hairy roots according to claim 1, characterized in that: The top of the adjusting rod (29) is rotatably connected to the bottom of the power cylinder (25). An intermediate groove (44) is formed inside the adjusting rod (29). The intermediate groove (44) is communicated with the inside of the power cylinder (25).

5. The airlift bioreactor for culturing tobacco hairy roots according to claim 4, characterized in that: A bottom cylinder (41) is fixedly installed at the bottom of the rotating cylinder (21). The bottom of the adjusting rod (S29) movably penetrates the bottom wall of the rotating cylinder (21) and the top wall of the bottom cylinder (41). An inner cylinder (43) is fixedly installed at the bottom of the adjusting rod (29). The inner cylinder (43) is communicated with the inside of the intermediate groove (44).

6. The airlift bioreactor for cultivating tobacco hairy roots according to claim 5, characterized in that: An arc-shaped baffle (46) is fixedly installed on the inner bottom wall of the bottom cylinder (41). A discharge groove (45) is formed in the side wall of the inner cylinder (43). The arc-shaped baffle (46) is attached to the outside of the inner cylinder (43), and the corresponding arc-shaped baffle (46) is attached to the outside of the corresponding discharge groove (45). A second one-way spray head (42) is fixedly installed on the side wall of the bottom cylinder (41), and the communication direction of the second one-way spray head (42) is from the inside of the bottom cylinder (41) to the outside.

7. An airlift bioreactor for cultivating tobacco hairy roots according to claim 1, characterized in that: The inflation assembly (3) includes a fixed ring (32). A support rod (33) is fixedly installed between the fixed ring (32) and the top of the reaction cylinder (1). An air inlet pipe (34) is fixedly installed on the side surface of the fixed ring (32), and the air inlet pipe (34) communicates with the inside of the fixed ring (32). The fixed ring (32) is rotatably connected between the power cylinder (25) and the top cylinder (24). A communication groove (36) is formed in the inside of the support plate (212), and the communication groove (36) communicates the upper and lower parts above the top cylinder (24). The lower part of the top cylinder (24) communicates with the inside of the rotating cylinder (21).

8. The air-lift bioreactor for cultivating tobacco hairy roots according to claim 1, characterized in that: A first one-way spray head (31) is fixedly installed on the outside of the rotating cylinder (21), and the communication direction of the first one-way spray head (31) is from the inside of the rotating cylinder (21) to the outside. A baffle (35) is fixedly installed at the bottom of the moving rod (214), and the baffle (35) is attached above the communication groove (36).

9. The airlift bioreactor for cultivating tobacco hairy roots according to claim 1, characterized in that: A feeding port (5) and a pressure balance valve (6) are fixedly installed on the top of the reaction cylinder (1). A discharge valve (7) is fixedly installed at the bottom of the reaction cylinder (1).

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