Insecticidal synergistic composition containing tea saponin and application thereof
Through the specific ratio combination of tea saponin and neonicotinoid insecticides, the drug resistance problems of cotton aphids and thrips in the west are solved, the insecticide effect is improved, and the amount of pesticides is reduced, and efficient pest control is achieved.
Patent Information
- Application Number
- CN202510833343.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-20
- Publication Date
- 2025-08-01
AI Technical Summary
The existing insecticides have serious drug resistance to cotton aphids and thrips. Tea saponin is poor in rapid effect and has a large amount of use when used alone, and lacks effective synergistic combination solutions.
Tea saponin is compounded with neonicotinic insecticide imidacloprid, flunarinil or ethyl polyvinylcin at a specific weight ratio to form an insecticidal and synergistic composition.
It significantly improves insecticidal activity, reduces the accumulation of resistance of pests to agents, reduces the use of pesticides and control costs, and provides a green prevention and control plan for cotton aphids and western flower thrips.
Smart Images
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Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of pest control, and particularly relates to an insecticidal synergistic composition containing tea saponin and its application. Background Art
[0002] Aphis gossypii and Frankliniella occidentalis are the main pests that damage crops such as cotton and melons, and their resistance to pesticides has become a major challenge in global agricultural production. At present, traditional insecticides for such pests, such as organophosphates, carbamates, pyrethroids and neonicotinoids, have generally become ineffective, and pests have developed serious resistance to them. For example, sulfoxaflor, as the first Sulfoximine insecticide, initially showed excellent control effects on piercing-sucking mouthpart pests, but recent monitoring data shows that the resistance multiples of Aphis gossypii in the northwestern inland cotton area and the Yellow River Basin cotton area of China have reached as high as 30 - 151 times; although spinetoram was once considered the most effective agent for controlling thrips, in the field populations in Hebei, Beijing, Henan, Shandong and other places, its resistance multiples have risen to 62.27 - 259.80 times, and in some areas it has even reached a high resistance level.
[0003] As a natural surfactant, tea saponin has the effects of contact killing, stomach poisoning and repelling pests, but when used alone, it has problems such as poor quick-acting property and large application amount, and the actual control effect is limited. In the prior art, there is no effective solution to synergistically combine tea saponin with insecticidal active ingredients such as imidacloprid, sulfoxaflor or spinetoram. Summary of the Invention
[0004] The present invention aims to provide an insecticidal synergistic composition containing tea saponin and its application. The insecticidal synergistic composition can effectively control pests and diseases, improve the insecticidal effect, and effectively delay the resistance development of Aphis gossypii and Frankliniella occidentalis to bactericides.
[0005] To solve the above technical problems, the technical solution adopted by the present invention is as follows:
[0006] An insecticidal synergistic composition containing tea saponin, comprising tea saponin and an insecticidal active ingredient, the insecticidal active ingredient includes but is not limited to one of neonicotinoid insecticides, sulfoxaflor or spinetoram, and the weight ratio of the tea saponin to the insecticidal active ingredient is 1:1 - 50:1.
[0007] The insecticidal active ingredient is a neonicotinoid insecticide, and the weight ratio of the tea saponin to the neonicotinoid insecticide is 1:1 - 50:1.
[0008] Preferably, the neonicotinoid insecticide includes one of imidacloprid, thiamethoxam, dinotefuran, nitenpyram.
[0009] Preferably, the insecticidal active ingredient is sulfoxaflor, and the weight ratio of the tea saponin to sulfoxaflor is 1:1 - 50:1.
[0010] Preferably, the insecticidal active ingredient is spinetoram, and the weight ratio of the tea saponin to spinetoram is 1:1 - 50:1.
[0011] The present invention also provides an application of the insecticidal synergistic composition as described above in controlling pests and diseases.
[0012] The present invention also provides an application of the insecticidal synergistic composition as described above in controlling Aphis gossypii and / or Frankliniella occidentalis.
[0013] The present invention also provides an insecticide, which is prepared from the insecticidal synergistic composition and agriculturally acceptable auxiliaries and / or carriers.
[0014] Compared with the prior art, the present invention has the following advantages and technical effects:
[0015] The present invention discloses an insecticidal synergistic composition containing tea saponin and its application. By compounding natural tea saponin with imidacloprid, sulfoxaflor or spinetoram in a specific weight ratio, the insecticidal activity is significantly improved. Compared with a single insecticidal active ingredient, the accumulation of resistance of Aphis gossypii or Frankliniella occidentalis to the medicament can be significantly reduced, and the usage amount of pesticides and the control cost of pests can be reduced. Through the synergistic effect of natural and chemical components, the present invention solves the problems of high resistance, large dosage and high environmental risk of traditional insecticides, and provides an efficient technical solution for the green control of Aphis gossypii and Frankliniella occidentalis.
[0016] The technical solution of the present invention will be further described in detail below through examples. Detailed Description of the Invention
[0017] The technical solution of the present invention will be further described below through examples.
[0018] Unless otherwise defined, the technical terms or scientific terms used in the present invention should have the ordinary meanings understood by those of ordinary skill in the art to which the present invention belongs.
[0019] The sulfoxaflor technical material was purchased from Dow AgroSciences, USA; the imidacloprid technical material, spinetoram technical material and tea saponin technical material were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.
[0020] In the present invention, unless otherwise specified, other test materials and instrument equipment are all conventional test materials in the art and can be obtained through commercial channels.
[0021] Example 1
[0022] Dissolve tea saponin and imidacloprid in water and DMSO, respectively, to prepare 1% stock solutions for later use. Dilute the tea saponin stock solution with a 0.1% Tween solution to 200, 400, 800, 1600, and 3200 mg / L; dilute the imidacloprid stock solution with a 0.1% Tween solution to 6.25, 12.5, 25, 50, and 100 mg / L. According to the ratio of imidacloprid solution to tea saponin solution in Table 1 below, the total concentrations of the test solutions were prepared as follows: imidacloprid: tea saponin = 1:1, 5, 10, 20, 40, 80 mg / L; imidacloprid: tea saponin = 1:10, 10, 20, 40, 80, 160 mg / L; imidacloprid: tea saponin = 1:50, 20, 40, 80, 160, 320 mg / L; imidacloprid: tea saponin = 10:1, 5, 10, 20, 40, 80 mg / L; imidacloprid: tea saponin = 50:1, 5, 10, 20, 40, 80 mg / L.
[0023] Directly immerse cotton leaves with cotton aphids in the test solution for 5 seconds, then take them out and place them in a clean culture dish covered with filter paper. After drying, seal the dish and place it in a (27±1)℃ light incubator for 48 hours to check the mortality rate.
[0024] The criterion for judging death was that the insects did not respond or responded uncoordinatedly when the needle was lightly touched. The mortality rate was calculated and the toxicity regression curve was fitted using DPS software to calculate the LC 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC) of the mixture, and the synergistic effect of the drugs was evaluated based on the calculated co-toxicity coefficient (CTC). A CTC ≤ 80 indicates an antagonistic effect, 80 < CTC < 120 indicates an additive effect, and CTC ≥ 120 indicates a synergistic effect. The results are shown in Table 1.
[0025] Table 1 Toxicity test results of imidacloprid and tea saponin at different ratios to cotton aphids
[0026]
[0027] Example 2
[0028] The original drugs of tea saponin and sulfoxaflor were separately dissolved in water and DMSO to prepare 1% stock solutions for standby. Then, the above tea saponin stock solution was diluted with an aqueous solution containing 0.1% Tween to 200, 400, 800, 1600, 3200 mg / L; the sulfoxaflor stock solution was diluted with an aqueous solution containing 0.1% Tween to 12.5, 25, 50, 100, 200 mg / L. And according to the ratio of sulfoxaflor liquid medicine to tea saponin liquid medicine in Table 2 below, the total concentration of the test liquid medicine was configured as follows: sulfoxaflor: tea saponin = 1:1, 3.125, 6.25, 12.5, 25, 50 mg / L; sulfoxaflor: tea saponin = 1:10, 3.125, 6.25, 12.5, 25, 50 mg / L; sulfoxaflor: tea saponin = 1:50, 13.125, 26.25, 32.5, 75, 150 mg / L; sulfoxaflor: tea saponin = 10:1, 6.25, 12.5, 25, 50, 100 mg / L; sulfoxaflor: tea saponin = 50:1, 1.75, 3.125, 6.25, 12.5, 25 mg / L.
[0029] The cotton leaves with cotton aphids were directly immersed in the test liquid medicine for 5 seconds and then taken out and placed in a clean petri dish lined with filter paper, dried, sealed, and cultured in a light incubator at (27 ± 1)°C for 48 hours, and then the mortality rate was checked. The death judgment criteria and calculation method were the same as in Example 1, and the results are shown in Table 2 below.
[0030] Table 2 Toxicity determination results of sulfoxaflor and tea saponin against cotton aphids under different ratios
[0031]
[0032]
[0033] As can be seen from Table 2, the synergistic coefficients of sulfoxaflor and tea saponin under the ratios of 1:1, 1:10, 1:50, 10:1, and 50:1 are all greater than 120, indicating a synergistic effect. Among them, the synergistic coefficients are the highest under the ratios of imidacloprid to tea saponin of 1:10 and 1:50, which are 717.58 and 635.92 respectively.
[0034] Example 3
[0035] Dissolve the crude tea saponin and the crude spinetoram in water and DMSO respectively to prepare 1% stock solutions for standby. Then dilute the above-mentioned tea saponin stock solution with an aqueous solution containing 0.1% Tween to 200, 400, 800, 2000, 4000 mg / L; dilute the spinetoram stock solution with an aqueous solution containing 0.1% Tween to 2, 4, 8, 16, 32 mg / L. And according to the ratio of spinetoram solution to tea saponin solution in Table 3 below, prepare the total concentration of the test solution as follows: spinetoram: tea saponin = 1:1, 1, 2, 4, 8, 16 mg / L; spinetoram: tea saponin = 1:10, 4, 8, 16, 32, 64 mg / L; spinetoram: tea saponin = 1:50, 3.125, 6.25, 12.5, 25, 50 mg / L; spinetoram: tea saponin = 10:1, 2, 4, 8, 16 mg / L; spinetoram: tea saponin = 50:1, 6, 12, 24, 48 mg / L.
[0036] Dip the kidney bean leaves directly into the test solution for 5 seconds, then take them out and place them in a clean petri dish lined with filter paper until they are dry. Then pick about 50 adult western flower thrips into each dish and place them in a (27 ± 1)°C light incubator for 48 hours, and then check the mortality rate. The death judgment standard and calculation method are the same as in Example 1, and the results are shown in Table 3 below.
[0037] Table 3 Toxicity determination results of spinetoram and tea saponin against western flower thrips under different ratios
[0038]
[0039] As can be seen from Table 3, the synergistic coefficients of spinetoram and tea saponin are all greater than 120 under the ratios of 1:1, 1:10, 1:50 and 10:1, indicating a synergistic effect. Among them, the synergistic coefficient is the highest under the ratio of spinetoram to tea saponin of 1:10, which is 488.57 respectively.
[0040] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions of the present invention or make equivalent replacements, and these modifications or equivalent replacements cannot make the modified technical solutions deviate from the spirit and scope of the technical solutions of the present invention.
Claims
1. An insecticidal synergistic composition containing tea saponin, characterized in that, Containing tea saponin and insecticidal active ingredients, the insecticidal active ingredients include, but are not limited to, one of neonicotinoid insecticides, sulfoxaflor or spinetoram, and the weight ratio of tea saponin to the insecticidal active ingredients is 1:1 - 50:
1.
2. The insecticidal synergistic composition according to claim 1, wherein The insecticidal active ingredient is a neonicotinoid insecticide, and the weight ratio of tea saponin to the neonicotinoid insecticide is 1:1 - 50:
1.
3. The insecticidal synergistic composition according to claim 2, wherein The neonicotinoid insecticide includes one of imidacloprid, thiamethoxam, dinotefuran, nitenpyram.
4. The insecticidal synergistic composition according to claim 1, characterized in that, The insecticidal active ingredient is sulfoxaflor, and the weight ratio of tea saponin to sulfoxaflor is 1:1 - 50:
1.
5. The pesticidal synergistic composition according to claim 1, characterized in that, The insecticidal active ingredient is spinetoram, and the weight ratio of tea saponin to spinetoram is 1:1 - 50:
1.
6. Use of the insecticidal synergistic composition according to any one of claims 1 - 5 in the control of pests and diseases.
7. Use of the insecticidal synergistic composition according to any one of claims 1 - 5 in the control of Aphis gossypii and / or Frankliniella occidentalis.
8. An insecticide, characterized in that, The insecticide is prepared from the insecticidal synergistic composition according to any one of claims 1 - 5 and agriculturally acceptable auxiliaries and / or carriers.