Traditional Chinese medicine composition for treating acute gonarthromeningitis and application thereof

Abstract

The invention relates to a traditional Chinese medicine composition for treating acute gonarthromeningitis. The traditional Chinese medicine composition is prepared from, by weight, 13-17 parts of radix angelicae pubescentis, 13-17 parts of poria cocos, 10-14 parts of rhizoma atractylodis macrocephalae, 13-17 parts of coix seeds, 7-11 parts of cassia twigs, 4-8 parts of rhizoma galangae, 4-8 parts of radix saposhnikoviae, 10-14 parts of radix achyranthis bidentatae, 10-14 parts of herba taxilli, 10-14 parts of cortex eucommiae, 7-11 parts of pawpaw, 10-14 parts of caulis spatholobi, 10-14 parts of rhizoma anemarrhenae, 10-14 parts of radix paeoniae alba, 13-17 parts of radix codonopsis and 4-8 parts of radix glycyrrhizae. The invention further provides application of the traditional Chinese medicine composition. The medicine has the effects of dispelling wind, eliminating dampness, relaxing tendons, relieving pain and strengthening muscles and bones. The monarch is radix angelicae pubescentis. The medicine is mainly used for expelling wind-damp and relieving arthralgia. Indian buead, largehead atractylodes rhizome, coix seed, cassia twig and galangal are combined to promote diuresis, excrete dampness, tonify spleen, warm yang and dispel cold for treatment. Divaricate saposhnikovia root strengthens wind dispelling treatment. The radix achyranthis bidentatae has the effects of relieving swelling, strengthening knees and guiding downward flow of blood; the traditional Chinese medicine composition is mainly used for relieving swelling, removing dampness and strengthening muscles and bones, has the effects of tonification and purgation and treats both symptoms and root causes.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and specifically, to a traditional Chinese medicine composition for treating acute knee synovitis and its application. Background Art

[0002] Acute knee synovitis is common in middle-aged and elderly patients. Most cases have knee osteoarthritis (KOA) as the underlying disease. Due to various reasons, there are acute knee swelling and pain, and the pain intensifies after walking and moving, with limited mobility. The tongue is light red, the coating is thin and white, and the pulse is slippery and thready. According to traditional Chinese medicine differentiation, it is knee bi syndrome with deficiency in origin and excess in superficiality. It is due to the deficiency of the body constitution, the syndrome of liver and kidney deficiency, and the invasion of the knee joint by wind-cold-damp pathogens.

[0003] Chinese patent document CN 111346190A discloses a traditional Chinese medicine composition for treating knee synovitis and its preparation method. On the basis of astragalus membranaceus, polygala tenuifolia, achyranthes bidentata, dendrobium officinale, and honeysuckle flower, this formula adds salvia miltiorrhiza, angelica sinensis, rhizoma sparganii, olibanum, and myrrh, which promote blood circulation, dredge stasis, and relieve pain; supplemented by fructus corni and codonopsis pilosula to tonify the liver and kidney and assist the healthy qi, and anemarrhena asphodeloides to clear heat; added poria cocos to strengthen the spleen and resolve dampness; the combination of olibanum and myrrh has the effect of promoting the flow of qi and blood in the meridians. Therefore, the whole formula has good effects of clearing heat and promoting diuresis, dredging the joints, and nourishing the liver and kidney. In animal experiments and clinical trials, this traditional Chinese medicine composition has a significant therapeutic effect on knee synovitis. Chinese patent document CN 105770846A discloses a traditional Chinese medicine combination for treating knee synovitis. The present invention is made from the following raw materials in grams by weight: 3 - 9 grams of arctium lappa, 3 - 9 grams of drynaria fortunei, 1.5 - 9 grams of manis pentadactyla, 9 - 30 grams of typha angustata, 3 - 9 grams of bletilla striata, 9 - 30 grams of bamboo juice, 1.5 - 9 grams of ginger, 6 - 15 grams of piper kadsura, 3 - 9 grams of eucommia ulmoides, and 5 - 10 grams of dipsacus asperoides. The traditional Chinese medicine combination adopted in the present invention can be prepared into common oral preparations by using existing traditional Chinese medicine technologies. The adopted drugs first disperse wind and clear heat, relieve pain, dredge meridians, activate blood stasis, eliminate carbuncles and swellings, cool blood and activate blood circulation, reduce swelling and remove necrosis, and promote granulation. Then, according to the condition, tonify the liver and kidney and strengthen the bones and muscles, which is beneficial to eliminating symptoms, improving the physical health of patients, and avoiding recurrence.

[0004] There are many such traditional Chinese medicine compositions for treating acute knee synovitis, but they all have more or less disadvantages. For example, there are too many medicinal flavors, difficult material collection, high prices; the medicinal effects are not obvious, and the treatment effects are poor, etc. Therefore, there is an urgent need for a drug with obvious effects, convenient preparation, and easy use for treating acute knee synovitis. Summary of the Invention

[0005] The purpose of the present invention is to provide a traditional Chinese medicine composition for treating acute knee synovitis and its application in view of the deficiencies in the prior art.

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating acute knee synovitis, which is prepared from the following raw materials in parts by weight: 13-17 parts of Angelica dahurica, 13-17 parts of Poria cocos, 10-14 parts of Atractylodes macrocephala, 13-17 parts of Coix seed, 7-11 parts of cinnamon twig, 4-8 parts of Alpinia officinalis, 4-8 parts of Saposhnikovia divaricata, 10-14 parts of Achyranthes bidentata, 10-14 parts of Morus alba, 10-14 parts of Eucommia bark, 7-11 parts of papaya, 10-14 parts of Millettia reticulata, 10-14 parts of Anemarrhena asphodeloides, 10-14 parts of White Peony Root, 13-17 parts of Codonopsis pilosula, and 4-8 parts of Licorice.

[0007] As a preferred example, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14-16 parts of Angelica dahurica, 14-16 parts of Poria cocos, 11-13 parts of Atractylodes macrocephala, 14-16 parts of Coix seed, 8-10 parts of cinnamon twig, 5-7 parts of Alpinia officinalis, 5-7 parts of Saposhnikovia divaricata, 11-13 parts of Achyranthes bidentata, 11-13 parts of Morus alba, 11-13 parts of Eucommia bark, 8-10 parts of papaya, 11-13 parts of Millettia reticulata, 11-13 parts of Anemarrhena asphodeloides, 11-13 parts of White Peony Root, 14-16 parts of Codonopsis pilosula, and 5-7 parts of Licorice.

[0008] As another preferred example, the Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of Angelica dahurica, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix seed, 9 parts of cinnamon twig, 6 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Morus alba, 12 parts of Eucommia ulmoides, 9 parts of papaya, 12 parts of Millettia reticulata, 12 parts of Anemarrhena asphodeloides, 12 parts of White Peony Root, 15 parts of Codonopsis pilosula, and 6 parts of Licorice.

[0009] In a second aspect, the present invention provides use of a traditional Chinese medicine composition in preparing a medicament for treating acute knee synovitis.

[0010] The advantages of the present invention are as follows: The treatment principle of the present invention is to dispel wind and dampness, relax muscles and relieve pain, and strengthen tendons and bones. The main ingredient is Angelica dahurica. It mainly dispels rheumatism and relieves arthralgia. The auxiliary ingredients are Poria, Atractylodes macrocephala, Coix seed, Cinnamon twig, and Alpinia officinalis, which are combined to promote diuresis, eliminate dampness, strengthen the spleen and warm yang, and dispel cold. Saposhnikovia divaricata strengthens the wind-dispelling treatment. Achyranthes bidentata reduces swelling, strengthens the knees, and guides blood downward, and is combined with Viscum album and Eucommia ulmoides to strengthen tendons and bones and nourish the liver and kidneys. Papaya and Millettia reticulata are combined to relax muscles and activate collaterals to lubricate the knee joints. The auxiliary ingredients are Paeonia lactiflora and Anemarrhena asphodeloides to nourish yin and protect fluid, and prevent warm and dry drugs from damaging yin and consuming body fluid. Codonopsis pilosula to balance qi and blood. The envoy is Licorice to harmonize the various medicines. The various medicines work together, with reducing swelling and dampness, strengthening tendons and bones as the main attack and supplement, to treat both the symptoms and the root causes. DETAILED DESCRIPTION

[0011] Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiments are only used to illustrate the present invention and are not used in limiting the scope of the present invention.In addition, should be understood that after reading the content of the present invention record, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall equally within the scope limited by the application's appended claims.

[0012] Example 1 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (I)

[0013] 15 parts of Angelica pubescens, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix lacryma-jobi, 9 parts of Cinnamomum cassia, 6 parts of Alpinia officinarum, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Taxillus chinensis, 12 parts of Eucommia ulmoides, 9 parts of Chaenomeles sinensis, 12 parts of Spatholobus suberectus, 12 parts of Anemarrhena asphodeloides, 12 parts of Paeonia lactiflora, 15 parts of Codonopsis pilosula, 6 parts of Glycyrrhiza uralensis.

[0014] Example 2 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (II)

[0015] 15 parts of Angelica pubescens, 14 parts of Poria cocos, 13 parts of Atractylodes macrocephala, 17 parts of Coix lacryma-jobi, 8 parts of Cinnamomum cassia, 5 parts of Alpinia officinarum, 7 parts of Saposhnikovia divaricata, 10 parts of Achyranthes bidentata, 14 parts of Taxillus chinensis, 12 parts of Eucommia ulmoides, 9 parts of Chaenomeles sinensis, 11 parts of Spatholobus suberectus, 12 parts of Anemarrhena asphodeloides, 12 parts of Paeonia lactiflora, 14 parts of Codonopsis pilosula, 6 parts of Glycyrrhiza uralensis.

[0016] Example 3 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (III)

[0017] 14 parts of Angelica pubescens, 16 parts of Poria cocos, 10 parts of Atractylodes macrocephala, 15 parts of Coix lacryma-jobi, 10 parts of Cinnamomum cassia, 7 parts of Alpinia officinarum, 4 parts of Saposhnikovia divaricata, 14 parts of Achyranthes bidentata, 12 parts of Taxillus chinensis, 11 parts of Eucommia ulmoides, 8 parts of Chaenomeles sinensis, 13 parts of Spatholobus suberectus, 11 parts of Anemarrhena asphodeloides, 11 parts of Paeonia lactiflora, 16 parts of Codonopsis pilosula, 5 parts of Glycyrrhiza uralensis.

[0018] Example 4 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (IV)

[0019] 16 parts of Angelica pubescens, 13 parts of Poria cocos, 14 parts of Atractylodes macrocephala, 14 parts of Coix lacryma-jobi, 7 parts of Cinnamomum cassia, 4 parts of Alpinia officinarum, 8 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 11 parts of Taxillus chinensis, 13 parts of Eucommia ulmoides, 10 parts of Chaenomeles sinensis, 10 parts of Spatholobus suberectus, 13 parts of Anemarrhena asphodeloides, 13 parts of Paeonia lactiflora, 13 parts of Codonopsis pilosula, 7 parts of Glycyrrhiza uralensis.

[0020] Example 5 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (V)

[0021] 13 parts of Angelica pubescens, 17 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 16 parts of Coix lacryma-jobi, 11 parts of Cinnamomum cassia, 8 parts of Alpinia officinarum, 6 parts of Saposhnikovia divaricata, 11 parts of Achyranthes bidentata, 13 parts of Taxillus chinensis, 10 parts of Eucommia ulmoides, 7 parts of Chaenomeles sinensis, 14 parts of Spatholobus suberectus, 10 parts of Anemarrhena asphodeloides, 10 parts of Paeonia lactiflora, 17 parts of Codonopsis pilosula, 4 parts of Glycyrrhiza uralensis.

[0022] Example 6 Traditional Chinese Medicine Composition for Acute Knee Synovitis of the Present Invention (VI)

[0023] 17 parts of Angelica dahurica, 15 parts of Poria, 11 parts of Atractylodes macrocephala, 13 parts of Coix seeds, 9 parts of cinnamon twig, 6 parts of Alpinia officinalis, 5 parts of Saposhnikovia divaricata, 13 parts of Achyranthes bidentata, 10 parts of Morus alba, 14 parts of Eucommia bark, 11 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 14 parts of Rhizoma Anemarrhenae, 14 parts of White Peony Root, 15 parts of Codonopsis pilosula, and 8 parts of Licorice.

[0024] Example 7 Chinese medicine composition for acute knee synovitis of the present invention (VII)

[0025] 15 parts of Angelica dahurica, 16 parts of Poria, 10 parts of Atractylodes macrocephala, 15 parts of Coix seeds, 10 parts of cinnamon twigs, 7 parts of Alpinia officinarum, 4 parts of Saposhnikovia divaricata, 14 parts of Achyranthes bidentata, 12 parts of Morus alba, 11 parts of Eucommia bark, 10 parts of Chaenomeles sinensis, 13 parts of Millettia reticulata, 11 parts of Rhizoma Anemarrhenae, 11 parts of White Peony Root, 13 parts of Codonopsis pilosula, and 5 parts of Licorice.

[0026] Example 8 Chinese medicine composition for acute knee synovitis of the present invention (VIII)

[0027] 14 parts of Angelica dahurica, 13 parts of Poria, 14 parts of Atractylodes macrocephala, 14 parts of Coix seeds, 7 parts of cinnamon twig, 4 parts of Alpinia officinalis, 8 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 11 parts of Morus alba, 13 parts of Eucommia bark, 7 parts of Chaenomeles sinensis, 10 parts of Millettia reticulata, 13 parts of Rhizoma Anemarrhenae, 13 parts of White Peony Root, 17 parts of Codonopsis pilosula, and 7 parts of Licorice.

[0028] Example 9 Chinese medicine composition for acute knee synovitis of the present invention (IX)

[0029] 16 parts of Angelica dahurica, 17 parts of Poria, 12 parts of Atractylodes macrocephala, 16 parts of Coix seeds, 11 parts of cinnamon twigs, 8 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 11 parts of Achyranthes bidentata, 13 parts of Morus alba, 10 parts of Eucommia bark, 11 parts of Chaenomeles sinensis, 14 parts of Millettia reticulata, 10 parts of Anemarrhena asphodeloides, 10 parts of White Peony Root, 15 parts of Codonopsis pilosula, and 4 parts of Licorice.

[0030] Example 10 Chinese medicine composition for acute knee synovitis of the present invention (Ten)

[0031] 13 parts of Angelica dahurica, 15 parts of Poria, 11 parts of Atractylodes macrocephala, 13 parts of Coix seeds, 9 parts of cinnamon twig, 6 parts of Alpinia officinalis, 5 parts of Saposhnikovia divaricata, 13 parts of Achyranthes bidentata, 10 parts of Morus alba, 14 parts of Eucommia bark, 9 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 14 parts of Rhizoma Anemarrhenae, 14 parts of White Peony Root, 14 parts of Codonopsis pilosula, and 8 parts of Licorice.

[0032] Example 11 Chinese medicine composition for acute knee synovitis of the present invention (XI)

[0033] 17 parts of Angelica dahurica, 14 parts of Poria, 13 parts of Atractylodes macrocephala, 17 parts of Coix seeds, 8 parts of cinnamon twig, 5 parts of Alpinia officinalis, 7 parts of Saposhnikovia divaricata, 10 parts of Achyranthes bidentata, 14 parts of Morus alba, 12 parts of Eucommia bark, 8 parts of Chaenomeles sinensis, 11 parts of Millettia reticulata, 12 parts of Rhizoma Anemarrhenae, 12 parts of White Peony Root, 16 parts of Codonopsis pilosula, and 6 parts of Licorice.

[0034] Example 12 Clinical Trial

[0035] 1 Clinical trial method

[0036] Select 30 patients with acute knee synovitis who visited the orthopedics department of Jing'an District Traditional Chinese Medicine Hospital from August 2022 to July 2023 and took orally the medicine in Example 1 of the present invention. Usage method: Decoct in water for oral administration, twice a day, 250 ml each time. 7 days is a course of treatment. Treat for 4 weeks.

[0037] 1.1 Diagnosis

[0038] Refer to the "Expert Consensus on the Diagnosis and Clinical Efficacy Evaluation of Adult Knee Synovitis" to formulate the western medicine diagnostic criteria for acute knee synovitis:

[0039] (1) Have a history of trauma or strain; (2) More common in the elderly and obese; (3) Knee joint swelling; (4) Knee joint pain; (5) Difficulty in flexion and extension of the knee joint; (6) The pressure pain point is not fixed; (7) The floating patella test is positive, and the skin temperature can be increased when touched; (8) The joint puncture fluid is light pink or light yellow liquid, and there is no fat on the surface.

[0040] Combined with clinical practice, after simultaneously meeting (3) and (4), and simultaneously meeting two other items, it can be diagnosed as acute knee synovitis.

[0041] 1.2 Inclusion criteria

[0042] (1) Meet the western medicine diagnostic criteria for acute knee synovitis and have been treated with oral administration of Xiaozhong Qiangxi Decoction for 3 weeks as required. (2) Meet the traditional Chinese medicine syndrome types of wind-cold-dampness obstruction and liver-kidney deficiency; (3) Age greater than 40 years old; (4) Exclude those with open injuries; (5) Undergo X-ray examination: no fractures, joint dislocations or bone diseases;

[0043] 1.3 Exclusion criteria

[0044] (1) Age < 40 years old; (2) Women in menstruation, pregnancy, lactation or preparing to get pregnant; (3) Patients with pain and swelling caused by rheumatism and bacterial infectious inflammation; (4) Synovitis caused by trauma or pathological fractures, dislocations or other bone diseases; (5) Patients with associated symptoms of meniscus tear and ligament rupture; (6) Patients with combined severe cardiovascular and cerebrovascular, tumor, endocrine or nervous system diseases, etc.; (7) Patients with obvious dysphagia, esophageal reflux, gastrointestinal ulcers or bleeding and other digestive system diseases that affect the patient's oral administration of decoction. (8) Patients with severe allergic diseases or allergic constitution; (9) Patients who cannot cooperate subjectively with the treatment; (10) Patients who have not signed the informed consent form;

[0045] 1.4 Exclusion criteria

[0046] (1) Those who did not complete the treatment as required after enrollment; (2) Patients who could not continue due to various reasons during the treatment. (3) Those with incomplete observation items and unable to evaluate the curative effect as required.

[0047] 1.5 Sample size of the study

[0048] This study belongs to a prospective self-before-and-after paired design. According to the calculation results, at least 20 patients need to be observed.

[0049] 1.6 Observation indicators

[0050] VAS score, JOA (Japanese Orthopaedic Association Assessment Treatment Score) knee osteoarthritis score, liver and kidney functions. Criteria for judging curative effect:

[0051] Calculate the improvement rate according to the JOA score. Improvement rate = (score after treatment - score before treatment) / (100 - score before treatment) × 100%. An improvement rate of 75% - 100% is excellent (marked effect), 50% - 74% is good (effective), 25% - 49% is fair (effective), and 0 - 24% is poor (ineffective). The total effective rate is calculated based on marked effect and effective.

[0052] 1.7 Observation time

[0053] After being included in the group, the curative effect is observed at two time points: at the time of enrollment and 4 weeks after treatment, and the JOA score is observed. All patients have their blood drawn to observe liver and kidney functions at the time of enrollment and 4 weeks after treatment.

[0054] 1.8 Statistical analysis

[0055] SPSS 26.0 software is used for statistical analysis of the data. Count data are described by frequency and percentage. Measurement data conforming to normal distribution are described by mean ± standard deviation, and paired sample t-test is used; non-normal distribution data are described by median M (interquartile range QR or P25, P75), and paired sample Wilcoxon rank sum test is used. P < 0.05 indicates that the difference is statistically significant. P < 0.01 indicates that the difference is significantly statistically significant.

[0056] 2 Research results

[0057] 2.1 General information

[0058] Thirty patients were included. (2 females and 1 male were excluded). Females accounted for 90% and males accounted for 10%. The average age was 58.4 years. The average onset time was 3.5 days. The distribution of the onset age of the patients is shown in Table 1.

[0059] Table 1 Distribution of the onset age of the patients

[0060]

[0061]

[0062] 2.2 Comparison of VAS scores before and after treatment

[0063] After treatment, the VAS scores of the patients were significantly improved, and the difference was significant (P<0.01). See Table 2.

[0064] Table 2 Comparison of VAS scores before and after treatment

[0065]

[0066] 2.3 Comparison of JOA scores before and after treatment

[0067] After treatment, the JOA scores of the patients were significantly improved, and there was a difference compared before and after treatment (P<0.05). See Table 3.

[0068] Table 3 Comparison of JOA scores before and after treatment

[0069]

[0070] 2.4 Curative effect results

[0071] Calculate the improvement rate according to the JOA score. Improvement rate = (score after treatment - score before treatment) / (100 - score before treatment) × 100%, an improvement rate of 75% - 100% is excellent (markedly effective), 50% - 74% is good (effective), 25% - 49% is fair (effective), 0 - 24% is poor (ineffective), and the total effective rate is calculated based on markedly effective and effective. After 4 weeks of treatment, 6 cases were markedly effective, 20 cases were effective, and 4 cases were ineffective, with a total effective rate of 86.7%. See Table 4

[0072] Table 4 Curative effect after treatment [case (%)]

[0073]

[0074] 2.5 Safety index situation

[0075] Among the 30 patients included in this study, during the treatment process, 2 patients had gastrointestinal reactions, manifested as nausea, decreased appetite, etc., but the treatment process was not interrupted and they still persisted in completing the treatment. There were no abnormalities in liver and kidney functions and other adverse reactions. See Table 5.

[0076] Table 5 Adverse reactions during treatment

[0077]

[0078] Example 13 Animal experiment

[0079] 1 Experimental materials

[0080] 1.1 Animals

[0081] Fifty-three male large-eared rabbits (2300-2900 g), aged 14-16 weeks, were housed in a clean animal room maintained at a temperature of 22-25°C and a humidity of 40%-70%. The rabbits were housed for approximately one week prior to the experiment to allow them to acclimate to the environment and were provided with free access to water and food.

[0082] 1.2.1 Main drugs for modeling

[0083] The present invention provides a Chinese medicine composition comprising 15 parts of Angelica dahurica, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix seeds, 9 parts of Cinnamon twig, 6 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Morus alba, 12 parts of Eucommia bark, 9 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 12 parts of Rhizoma Anemarrhenae, 12 parts of Paeonia lactiflora, 15 parts of Codonopsis pilosula, and 6 parts of Licorice root. The composition is administered orally twice daily for 4 weeks.

[0084] The second Chinese medicine composition of the present invention is as follows: 15 parts of Angelica dahurica, 14 parts of Poria cocos, 13 parts of Atractylodes macrocephala, 17 parts of Coix seeds, 8 parts of Cinnamon twig, 5 parts of Alpinia officinalis, 7 parts of Saposhnikovia divaricata, 10 parts of Achyranthes bidentata, 14 parts of Morus alba, 12 parts of Eucommia bark, 9 parts of Chaenomeles sinensis, 11 parts of Millettia reticulata, 12 parts of Rhizoma Anemarrhenae, 12 parts of Paeonia lactiflora, 14 parts of Codonopsis pilosula, and 6 parts of Licorice root are weighed in proportion by weight. The composition is taken orally twice a day for 4 weeks.

[0085] Control group: Traditional Chinese medicine composition: 15 parts of Angelica dahurica, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix seed, 9 parts of Magnolia florid, 6 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Morus parasiticus, 12 parts of Eucommia bark, 9 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 12 parts of Ophiopogon japonicus, 12 parts of White Peony Root, 15 parts of Codonopsis pilosula, and 6 parts of Licorice root were weighed and administered orally twice daily for 4 weeks.

[0086] Control group 2: Traditional Chinese medicine composition: 15 parts of Angelica dahurica, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix seed, 9 parts of Cinnamon twig, 6 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Morus alba, 12 parts of Eucommia barbata, 9 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 12 parts of Ophiopogon japonicus, 12 parts of Angelica sinensis, 15 parts of Codonopsis pilosula, and 6 parts of Licorice root were weighed and administered orally twice daily for 4 weeks.

[0087] Control group three: Traditional Chinese medicine composition: 15 parts of Angelica dahurica, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix seed, 9 parts of Magnolia florid, 6 parts of Alpinia officinalis, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Morus alba, 12 parts of Eucommia barbata, 9 parts of Chaenomeles sinensis, 12 parts of Millettia reticulata, 12 parts of Anemarrhena asphodeloides, 12 parts of Angelica sinensis, 15 parts of Codonopsis pilosula, and 6 parts of Licorice root. The composition was administered orally twice daily for 4 weeks.

[0088] Fourth traditional Chinese medicine composition of the control group: Weigh 15 parts of Angelica pubescens, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix lacryma-jobi, 9 parts of Magnolia liliflora Desr., 6 parts of Alpinia officinarum Hance, 6 parts of Saposhnikovia divaricata (Turcz.) Schischk., 12 parts of Achyranthes bidentata Blume, 12 parts of Taxillus chinensis (DC.) Danser, 12 parts of Eucommia ulmoides Oliv., 9 parts of Chaenomeles sinensis (Thouin) Koehne, 12 parts of Spatholobus suberectus Dunn, 12 parts of Ophiopogon japonicus (Thunb.) Ker-Gawl., 12 parts of Angelica sinensis (Oliv.) Diels, 15 parts of Codonopsis pilosula (Franch.) Nannf., and 6 parts of Glycyrrhiza uralensis Fisch. per weight portion. Administer by gavage twice a day for 4 weeks of treatment.

[0089] 1.3 Method for establishing the model of acute knee synovitis

[0090] Six big ear rabbits were selected and divided into the blank group, and normal saline was injected daily. The remaining 47 big ear rabbits were used for modeling.

[0091] The specific method is as follows: Fix the big ear rabbit, cut the hair around the knee joint of the left hind limb (modeling limb) with scissors, and remove the remaining hair with 8% sodium sulfide to expose the skin. Anesthetize the rabbit by slowly injecting 3% pentobarbital sodium at 1 mL / kg through the marginal ear vein, then place the rabbit on its side on the experimental table with the modeling limb facing up. Vertically insert an acupuncture needle into the midpoints of the quadriceps femoris and gastrocnemius of the modeling limb of the rabbit. Connect the electroacupuncture therapeutic instrument, select the continuous wave waveform frequency, and continuously and passively flex and extend the knee joint to cause damage to the knee synovitis, once a day, 90 minutes each time, for 10 consecutive days. Measure the increase in the joint circumference and the increase in skin temperature to confirm the successful establishment of the animal model of traumatic knee synovitis in rabbits.

[0092] Modeling results of 47 big ear rabbits: 42 big ear rabbits were successfully modeled, and 5 big ear rabbits failed to be modeled. The 42 successfully modeled big ear rabbits were randomly divided into groups of 6 each. One group was used as the model group without administration, and the other 6 groups were administered in the above manner.

[0093] 1.4 Observation indicators

[0094] Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were detected by enzyme-linked immunosorbent assay.

[0095] 2 Statistical analysis

[0096] Analysis and processing were performed using SPSS 16.0 statistical software. The data were expressed as (x±s), and the t-test was used for comparison between groups.

[0097] 3 Results

[0098] 3.1 As shown in Table 6.

[0099] Table 6: Comparison of the expressions of IL-6 and TNF-α in the knee joint fluid of rabbits in each group (x-±s, ng / L)

[0100]

[0101] Note: Compared with the blank group 1) P < 0.05, compared with the model group 2) P < 0.05, compared with control group 2 3) P < 0.05, compared with control group 3 4) P < 0.05, compared with control group 4 5) P < 0.05, compared with control group 1 6) P < 0.05, compared with the second group of the present invention 7) P < 0.05

[0102] Efficacy comparison: The first group of the present invention > the second group of the present invention > the first control group > the second control group > the third control group > the fourth control group. The first group of the present invention has the best efficacy. In summary, the efficacy of the present invention group is definite; the efficacy is the best

[0103] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the method of the present invention, several improvements and supplements can be made, and these improvements and supplements should also be regarded as the protection scope of the present invention

Claims

1. A traditional Chinese medicine composition for treating acute knee synovitis, characterized in that, The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 13-17 parts of Heracleum hemsleyanum, 13-17 parts of Poria cocos, 10-14 parts of Atractylodes macrocephala, 13-17 parts of Coix lacryma-jobi, 7-11 parts of Cinnamomum cassia, 4-8 parts of Alpinia officinarum, 4-8 parts of Saposhnikovia divaricata, 10-14 parts of Achyranthes bidentata, 10-14 parts of Taxillus chinensis, 10-14 parts of Eucommia ulmoides, 7-11 parts of Chaenomeles sinensis, 10-14 parts of Spatholobus suberectus, 10-14 parts of Anemarrhena asphodeloides, 10-14 parts of Paeonia lactiflora, 13-17 parts of Codonopsis pilosula, 4-8 parts of Glycyrrhiza uralensis.

2. The traditional Chinese medicine composition according to claim 1, wherein The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 14-16 parts of Heracleum hemsleyanum, 14-16 parts of Poria cocos, 11-13 parts of Atractylodes macrocephala, 14-16 parts of Coix lacryma-jobi, 8-10 parts of Cinnamomum cassia, 5-7 parts of Alpinia officinarum, 5-7 parts of Saposhnikovia divaricata, 11-13 parts of Achyranthes bidentata, 11-13 parts of Taxillus chinensis, 11-13 parts of Eucommia ulmoides, 8-10 parts of Chaenomeles sinensis, 11-13 parts of Spatholobus suberectus, 11-13 parts of Anemarrhena asphodeloides, 11-13 parts of Paeonia lactiflora, 14-16 parts of Codonopsis pilosula, 5-7 parts of Glycyrrhiza uralensis.

3. The traditional Chinese medicine composition according to claim 1, wherein The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 15 parts of Heracleum hemsleyanum, 15 parts of Poria cocos, 12 parts of Atractylodes macrocephala, 15 parts of Coix lacryma-jobi, 9 parts of Cinnamomum cassia, 6 parts of Alpinia officinarum, 6 parts of Saposhnikovia divaricata, 12 parts of Achyranthes bidentata, 12 parts of Taxillus chinensis, 12 parts of Eucommia ulmoides, 9 parts of Chaenomeles sinensis, 12 parts of Spatholobus suberectus, 12 parts of Anemarrhena asphodeloides, 12 parts of Paeonia lactiflora, 15 parts of Codonopsis pilosula, 6 parts of Glycyrrhiza uralensis.

4. Use of the traditional Chinese medicine composition according to any one of claims 1-3 in the preparation of a medicament for treating acute knee synovitis.

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