Amino acid-containing water-soluble fertilizer and preparation method thereof

Abstract

The invention relates to the technical field of fertilizers, and discloses an amino acid-containing water-soluble fertilizer and a preparation method thereof, and the method comprises the steps of raw material preparation, carrier system construction, signal molecule extraction and activation, stress-resistant amino acid compound preparation, multi-element synergistic system construction and the like. Through innovative combination of a soil penetrating carrier system and a plant source signal molecule technology, the technical problems of weak penetrating power, low bioavailability and the like of active components of a fertilizer in saline-alkali soil are solved, so that the effective penetrating depth of signal molecules in severe saline-alkali soil is effectively improved, the bioavailability is improved, and the application prospect is wide. The saline-alkali resistance of the plants is obviously enhanced, and the ecological restoration and sustainable utilization of the saline-alkali soil are promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and more specifically, it relates to an amino acid-containing water-soluble fertilizer and a preparation method thereof. Background Art

[0002] The soil environment of saline-alkali land is characterized by high pH value, high salt content, low organic matter content, etc., which severely restricts crop growth and leads to a significant decline in yield and quality. At present, the improvement of saline-alkali land mainly faces the following technical problems: the active ingredients in traditional fertilizers are easily fixed in saline-alkali soil and are difficult to be effectively absorbed by plants; the surface charge density of saline-alkali soil particles is high, which has a strong adsorption effect on the charged fertilizer active ingredients, resulting in the difficulty of the fertilizer to penetrate into the effective absorption area of plant roots; plants have insufficient self-stress resistance under saline-alkali stress and cannot fully exert their stress resistance ability; the existing amino acid water-soluble fertilizers mainly improve the stress resistance of plants through a single way of exogenous supplement of nutrients, with poor persistence of action and limited effects.

[0003] Although amino acid-based water-soluble fertilizers are widely used in agricultural production due to their high biological activity and easy absorption by plants, they still face technical bottlenecks such as poor penetration ability, insufficient stability, and single action pathway in the application of saline-alkali soil, and there is an urgent need to develop innovative amino acid water-soluble fertilizers targeting the characteristics of saline-alkali soil. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides an amino acid-containing water-soluble fertilizer, which comprises the following raw materials in parts by weight:

[0005] 15-25 parts of stress resistance-related amino acids, 8-15 parts of salt-tolerant plant raw materials, 10-15 parts of humic acid, 8-12 parts of seaweed extract, 5-8 parts of medium-chain fatty acids, 2-4 parts of organosilicon surface active substances, and 2-3 parts of biogenic wetting agents.

[0006] Preferably: the stress resistance-related amino acids include L-proline, betaine, glutamic acid, and glycine, and are mixed in a mass ratio of 3:3:2:2.

[0007] Preferably: the salt-tolerant plant raw materials are selected from Salicornia europaea or Suaeda glauca growing in high-saline-alkali environments.

[0008] A preparation method of an amino acid-containing water-soluble fertilizer comprises the following steps:

[0009] Step 1: Obtain raw materials according to the ratio;

[0010] Step 2: Construct a soil penetration carrier system, including: activation of medium-chain fatty acids, construction of amphiphilic carriers, integration of biogenic wetting agents, and sol-gel transformation to form a nano-scale dispersion with a particle size of 50-200 nm;

[0011] Step 3: Extract and activate plant-derived signal molecules from salt-tolerant plant raw materials;

[0012] Step 4: Prepare stress-resistant amino acid complexes;

[0013] Step 5: Compound humic acid with seaweed extract, and then integrate it with plant-derived signal molecules, a soil penetration carrier system, and stress-resistant amino acid complexes to construct a multi-component synergistic system.

[0014] Preferably: The steps for activating medium-chain fatty acids include: carrying out an esterification reaction on medium-chain fatty acids and alcohols at a molar ratio of 1:1.2 - 1.5 for 4 - 6 hours at 55 - 65 °C to generate an amphiphilic molecular precursor with an HLB value of 8 - 12.

[0015] Preferably: The steps for constructing an amphiphilic carrier include: compounding the activated medium-chain fatty acids with an organosilicon surfactant at a mass ratio of 1:0.3 - 0.5 at 40 - 50 °C, and treating it with a high-shear emulsification technique at 8000 - 10000 r / min for 15 - 25 minutes.

[0016] Preferably: The steps for sol-gel transformation include: adding polyvalent metal ions or natural polysaccharides as cross-linking agents for gelation under the conditions of pH 5.5 - 6.5 and temperature 25 - 35 °C.

[0017] Preferably: The extraction steps for plant-derived signal molecules include: adding a water-ethanol mixed solvent (water:ethanol = 7:3) at a solid-liquid ratio of 1:8 - 10, and extracting for 4 - 6 hours at 35 - 45 °C using a biomimetic extraction technique, where the pH value is controlled at 5.8 - 6.2 and the redox potential is controlled at 150 - 200 mV.

[0018] Preferably: The activation steps for plant-derived signal molecules include: adding amino acids to form a hydrogen bond complex, performing hydroxymethylation or carboxymethylation modification on the signal molecules, and protecting them through cyclodextrin inclusion or polysaccharide embedding, so that the signal molecules still maintain more than 90% of their biological activity for more than 3 months in a 60 °C environment.

[0019] Preferably: The steps for preparing stress-resistant amino acid complexes include: carrying out amino acid activation treatment for 2 - 3 hours at pH 6.0 - 7.0 and temperature 30 - 40 °C; adding trace element chelates to form amino acid-trace element complexes; adding stabilizers and adjusting the pH value to 6.0 - 6.5.

[0020] Preferably: The trace element chelates include 0.5 - 0.8% iron chelate, 0.3 - 0.5% zinc chelate, 0.2 - 0.3% manganese chelate, 0.1 - 0.2% copper chelate, 0.1 - 0.2% boron, and 0.05 - 0.1% molybdenum, and the total addition amount is 1 - 3%.

[0021] Preferably, the steps for constructing a multi - element collaborative system include: uniformly mixing each component through programmed temperature - controlled mixing or multi - stage emulsification; adjusting the pH value to 6.5 - 7.0, the viscosity to 50 - 100 mPa·s, and the surface tension to 30 - 40 mN / m; and storing at low temperature under the condition of 4 - 8°C.

[0022] Preferably, it further includes the final product processing steps, making a liquid fertilizer through direct packaging, or making a concentrated liquid fertilizer through low - temperature concentration technology, or making a water - soluble powder through spray - drying technology.

[0023] The beneficial effects of the present invention are as follows: The present invention significantly improves the penetration ability and bioavailability of the active ingredients of fertilizers in saline - alkali soil. Through the nano - level carrier system, the effective penetration depth of signal molecules in severely saline - alkali soil (pH > 9.5) is increased from the traditional 5 - 8 cm to 25 - 30 cm, and the bioavailability is increased by more than 300%, significantly improving the utilization efficiency of fertilizers.

[0024] Successfully activates the plant's own stress - resistance response and establishes a new way to trigger the expression of the plant's own stress - resistance genes. The plant - derived signal molecules can activate the anti - saline - alkali defense system in plants, form an internal and external synergistic effect with the externally added stress - resistance amino acids, enabling the plants to maintain a relatively high survival rate in highly saline - alkali soil (pH > 9.0), with the plant's anti - saline - alkali ability increased by more than 60% and the crop yield increased significantly.

[0025] Significantly improves the stability and long - term effectiveness of fertilizers. Through multiple activation technologies, the plant - derived signal molecules can still maintain more than 90% of their biological activity for more than 3 months in an environment of 60°C; through the compounding process, the effective action time of the fertilizer in saline - alkali soil is extended to 2 - 3 times that of conventional fertilizers, reducing the number of fertilizations and lowering the labor cost.

[0026] Effectively improves the saline - alkali soil environment. Humic acid and seaweed extracts can improve the saline - alkali soil structure, enhance the soil's water - retaining and fertilizer - retaining capacity, and promote the reproduction of beneficial microorganisms. After two years of continuous use, the soil pH value can be stably reduced by 0.5 - 1.0 unit, the salt leaching rate is increased by 45%, and the soil organic matter content is increased by more than 30%, forming a virtuous cycle.

[0027] Demonstration applications show that after the amino - acid - containing water - soluble fertilizer prepared by the present invention is applied in saline - alkali areas, the yields of typical crops (such as wheat, rice, corn, etc.) are increased by 25 - 40%, the quality is significantly improved, and the drought - resistance, cold - resistance, and disease - resistance abilities are significantly enhanced. The farming cycle of saline - alkali land can be extended from the traditional single - season to annual planting, greatly improving the utilization value and economic benefits of saline - alkali land.

[0028] In summary, through the organic combination of multiple innovative technologies, the present invention has developed an efficient amino acid water-soluble fertilizer suitable for saline-alkali soil, which not only solves the technical problem of poor application effect of traditional fertilizers in saline-alkali soil, but also realizes the ecological restoration and sustainable utilization of saline-alkali soil, and has significant technical value and application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is the comparison of the effective penetration depth of the present invention;

[0030] Figure 2 is the expression level of salt-alkali resistance-related genes of the present invention;

[0031] Figure 3 is the comparison of the growth conditions of wheat under different concentrations of saline-alkali stress of the present invention;

[0032] Figure 4 is the degradation kinetic curve of different samples of the present invention in saline-alkali soil;

[0033] Figure 5 is the cumulative nutrient release curve of different samples of the present invention under simulated precipitation conditions;

[0034] Figure 6 is the nutrient release rate curve of different samples of the present invention;

[0035] Figure 7 is the comparison of the fertilizer efficiency persistence of different samples during the wheat growth period of the present invention:. DETAILED DESCRIPTION OF THE INVENTION

[0036] Now, the subject matter described herein will be discussed with reference to exemplary embodiments. It should be understood that discussing these embodiments is only to enable those skilled in the art to better understand and thus implement the subject matter described herein, and the functions and arrangements of the elements discussed can be changed without departing from the protection scope of the content of this specification. Each example can omit, substitute or add various processes or components as needed. Additionally, the features described in some examples can also be combined in other examples.

[0037] In at least one embodiment of the present invention, a water-soluble fertilizer containing amino acids is disclosed, which comprises the following raw materials in parts by weight:

[0038] 15 parts of stress-resistant related amino acids, 8 parts of salt-tolerant plant raw materials, 10 parts of humic acid, 8 parts of seaweed extract, 5 parts of medium-chain fatty acids, 2 parts of organosilicon surfactant, and 2 parts of biogenic wetting agent.

[0039] Among them, the stress-resistant related amino acids include L-proline, betaine, glutamic acid and glycine, which are mixed in a mass ratio of 3:3:2:2. The salt-tolerant plant raw material is selected from Salicornia europaea or Suaeda glauca growing in high-saline-alkali environments.

[0040] In at least one embodiment of the present invention, a water-soluble amino acid fertilizer is disclosed, which comprises the following raw materials in parts by weight:

[0041] 20 parts of stress-resistant related amino acids, 11 parts of salt-tolerant plant raw materials, 13 parts of humic acid, 10 parts of seaweed extract, 7 parts of medium-chain fatty acids, 3 parts of organosilicon surfactant, and 2.5 parts of biogenic wetting agent.

[0042] In at least one embodiment of the present invention, a water-soluble amino acid fertilizer is disclosed, which comprises the following raw materials in parts by weight:

[0043] 25 parts of stress-resistant related amino acids, 15 parts of salt-tolerant plant raw materials, 15 parts of humic acid, 12 parts of seaweed extract, 8 parts of medium-chain fatty acids, 4 parts of organosilicon surfactant, and 3 parts of biogenic wetting agent.

[0044] In at least one embodiment of the present invention, a preparation method of a water-soluble amino acid fertilizer comprises the following specific steps:

[0045] 1 Raw material preparation

[0046] Prepare raw materials in the following proportions: 20 parts of stress-resistant related amino acids, 11 parts of salt-tolerant plant raw materials, 13 parts of humic acid, 10 parts of seaweed extract, 7 parts of medium-chain fatty acids, 3 parts of organosilicon surfactant, 2.5 parts of biogenic wetting agent, and appropriate amounts of other auxiliary components (stabilizers, pH regulators, etc.).

[0047] Among them, the stress-resistant related amino acids are selected from L-proline, betaine, glutamic acid and glycine, and are proportioned according to the optimal ratio determined by experiments under saline-alkali stress of plants; the salt-tolerant plant raw materials are selected from Salicornia europaea, Suaeda glauca, etc. that can grow normally in high-saline-alkali environments (pH value > 8.5, salt content > 0.3%) to ensure that the extracted signal molecules have strong anti-saline-alkali activities; the humic acid is selected from potassium-type or sodium-type humic acid to improve its stability and activity in the water-soluble fertilizer; the seaweed extract is selected from brown algae to ensure that the content of alginate and polysaccharides reaches more than 60%.

[0048] 2 Carrier system construction

[0049] Specifically, it includes:

[0050] Medium-chain fatty acid activation: Esterification reaction is carried out on medium-chain fatty acids (caprylic acid, capric acid, etc.) and alcohols (propylene glycol, glycerol, etc.) at a molar ratio of 1:1.2 or 1:1.3 or 1:1.5 for 4 or 5 or 6 hours at 55 or 60 or 65 °C to generate amphiphilic molecular precursors with a hydrophilic-lipophilic balance value (HLB value of 8 or 10 or 12);

[0051] In this implementation: Esterification reaction is carried out on medium-chain fatty acids (caprylic acid, capric acid, etc.) and alcohols (propylene glycol, glycerol, etc.) at a molar ratio of 1:1.3 for 5 hours at 60 °C to generate amphiphilic molecular precursors with a hydrophilic-lipophilic balance value (HLB value of 10).

[0052] The key to this reaction lies in controlling the reaction temperature and time so that the generated amphiphilic molecular precursors have a molecular weight distribution of 500 - 800 Da and an HLB value of 8 - 12 to ensure their subsequent weak bonding ability with signal molecules and soil colloid penetration ability.

[0053] Construction of amphiphilic carrier: The activated medium-chain fatty acids and organosilicon surfactants (polysiloxane copolymers, etc.) are compounded at a mass ratio of 1:0.3 or 1:0.4 or 1:0.5 at 40 or 45 or 50 °C, and treated with high-shear emulsification technology (rotation speed of 8000 or 9000 or 10000 r / min) for 15 or 20 or 25 minutes to form a preliminarily stable emulsion.

[0054] In this implementation: The activated medium-chain fatty acids and organosilicon surfactants (polysiloxane copolymers, etc.) are compounded at a mass ratio of 1:0.4 at 45 °C, and treated with high-shear emulsification technology (rotation speed of 9000 r / min) for 20 minutes to form a preliminarily stable emulsion.

[0055] This step enables the uniform distribution of organosilicon surfactants on the surface of amphiphilic molecules through high-shear emulsification technology to form a composite carrier with a unique structure.

[0056] Integration of biogenic wetting agents: Biogenic wetting agents (phytosaponins, algal polysaccharides, etc.) are added to the above emulsion. The mass ratio of the wetting agent to the amphiphilic carrier is 0.2 - 0.4:1, and gentle stirring is carried out at 30 - 40 °C for 2 - 4 hours to enable the full combination of the wetting agent and the amphiphilic carrier. The key to this step lies in selecting biogenic wetting agents with soil affinity and making them uniformly distributed on the surface of the carrier through gentle stirring (200 - 300 r / min) to form a composite system with soil affinity.

[0057] Sol-gel transformation: The above mixture is subjected to sol-gel transformation at pH 5.5 or 6.0 or 6.5 and temperature 25 or 30 or 35 °C; in this example: The above mixture is subjected to sol-gel transformation at pH 6.0 and temperature 30 °C.

[0058] By adding a cross-linking agent (polyvalent metal ions such as Ca 2+ , Mg 2+ , Fe 3+ , with a concentration of 0.01 - 0.05 mol / L, or natural polysaccharides such as chitin, chitosan, with a concentration of 0.5 - 1.5%), the degree of gelation is controlled, and finally a nano-dispersion with a particle size of 50 - 200 nm is formed. The innovation of this step lies in achieving the nano-sizing of the carrier system by precisely controlling the sol-gel transformation conditions, significantly improving its soil penetration ability.

[0059] The carrier system prepared by the above steps has the following characteristics: One end forms a weak bond with the signal molecule, and the other end has the ability to penetrate soil colloids; as a whole, it forms a nano-dispersion, which can effectively avoid the strong adsorption of saline-alkali soil colloids; it has soil affinity and can release active ingredients directionally under the action of root exudates. These characteristics together enable the carrier system to carry fertilizer active ingredients through the saline-alkali soil colloid layer, significantly improving the bioavailability of the active ingredients.

[0060] 3 Signal molecule extraction and activation

[0061] Specifically include:

[0062] Halophyte extraction: The halophyte raw materials (such as Salicornia europaea, Suaeda salsa, etc.) are washed and chopped, and added to the extraction solvent (a mixture of water and organic solvent, water:ethanol = 7:3) according to a solid-liquid ratio of 1:8 or 1:9 or 1:10 (in this example, according to 1:9), and extracted for 4 or 5 or 6 hours (in this example, extracted for 5 hours) at 35 or 40 or 45 °C (in this example, the temperature is 40 °C) using bionic extraction technology. Bionic extraction technology refers to simulating the environmental conditions of information transmission between plants in nature for extraction, including controlling the pH value in the range of 5.8 or 6.0 or 6.2 (in this example, the pH value is controlled at 6.0), controlling the oxidation-reduction potential (ORP) in the range of 150 - 200 mV, and light conditions (using a light source simulating natural spectrum, with a light intensity of 5000 - 8000 lux), etc. The innovation of this step lies in that the plant hormone-like signal molecules obtained by bionic extraction technology retain more complete biological activity.

[0063] Signal molecule separation: The above extract is processed through separation processes (such as molecular sieve chromatography, membrane separation, etc.) to separate components rich in phytohormone signal molecules (jasmonic acid, salicylic acid, etc.) and polypeptides. According to the molecular weight and polarity characteristics of different signal molecules, different separation conditions are adopted. For jasmonic acid compounds, a C18 reverse-phase chromatography column is used, with the mobile phase being methanol-water-acetic acid (60:39:1) and the flow rate being 1.0 ml / min; for salicylic acid compounds, silica gel column chromatography is used, with the mobile phase being n-hexane-ethyl acetate (3:1) and the flow rate being 0.8 ml / min; for polypeptides, an ultrafiltration membrane (with a molecular weight cut-off value of 500 - 1000 Da) is used for separation, and the operating pressure is 0.2 - 0.3 MPa.

[0064] Signal molecule activation: The separated signal molecule components are processed through an activation process to improve their stability and activity. The activation process includes: (a) forming a hydrogen bond complex with the signal molecule by adding amino acids (such as alanine, leucine, etc., with a mass ratio of signal molecule:amino acid = 1:0.5 - 1.0) to improve its stability; (b) modifying the non-critical functional groups of the signal molecule through mild molecular modification techniques (such as hydroxymethylation, carboxymethylation, etc.), with the modification degree controlled at 10 - 20% to improve its binding ability with the carrier system; (c) protecting the signal molecule through an embedding technique (such as cyclodextrin inclusion, polysaccharide embedding, etc., with the embedding rate controlled at 60 - 80%) to improve its stability in a high-temperature environment. The innovation of this step lies in significantly improving the stability and activity of plant-derived signal molecules through multiple activation techniques, enabling them to maintain more than 90% of their biological activity for more than 3 months in a 60°C environment.

[0065] The plant-derived signal molecules prepared by the above steps have the following characteristics: They have high biological activity and can effectively trigger the expression of the plant's own stress-resistant genes; they have good stability and can still maintain activity at higher temperatures; they can effectively bind to the carrier system to achieve directional transmission and release; they have a complementary and synergistic effect with stress-resistant amino acids, significantly improving the overall effect of fertilizers.

[0066] Preparation of the 4 stress-resistant amino acid complex

[0067] This step mainly processes stress-resistant related amino acids through a process to make them have higher biological activity and better stability, specifically including:

[0068] Amino acid activation: Mix the stress-resistant related amino acids (proline, betaine, glutamic acid, and glycine) in a mass ratio of 3:3:2:2, and conduct activation treatment for 2 - 3 hours under the conditions of pH 6.0 - 7.0 and temperature 30 - 40°C. The activation treatment includes: adjusting the interaction between amino acid molecules to form a cyclic structure or a coiled structure to improve their biological activity; adding cofactors (trace elements such as Fe2+ , Zn 2+ Concentration 0.1 - 0.2%, vitamin B group 0.01 - 0.05%), promoting the interaction between amino acids and plant cells; adopting ultrasonic-assisted dissolution technology (power 300 - 500W, intermittent treatment for 5 - 10 minutes) to improve the solubility and stability of amino acids in water-soluble fertilizers.

[0069] Trace element chelation: Adding trace element chelates (chelates of iron, zinc, manganese, copper, etc.) to the activated amino acid mixture to form amino acid-trace element complexes. The selection and proportion of trace elements are determined according to the needs of typical crops in saline-alkali soil, including iron chelate 0.5 - 0.8%, zinc chelate 0.3 - 0.5%, manganese chelate 0.2 - 0.3%, copper chelate 0.1 - 0.2%, boron 0.1 - 0.2%, molybdenum 0.05 - 0.1%, and the total addition amount is 1 - 3%. This complexing process is carried out at pH 5.5 - 6.5 and temperature 25 - 35°C for 3 - 5 hours to form a stable chelate structure between trace elements and amino acids.

[0070] Stabilization of amino acid complexes: By adding stabilizers (citric acid 0.5 - 1.0%, sorbitol 1.0 - 2.0%, etc.) and adjusting the pH value to 6.0 - 6.5, the stability of amino acid complexes is improved. This step reduces the denaturation risk of the complex in a saline-alkali environment and extends the shelf life by adjusting the molecular structure and surface charge distribution of the complex. The operating temperature is controlled at 20 - 25°C, and the stirring time is 1 - 2 hours to finally obtain a stable stress-resistant amino acid complex.

[0071] The stress-resistant amino acid complex prepared by the above steps has the following characteristics: containing the optimal amino acid combination required by plants under saline-alkali stress; trace elements exist in chelated form with high biological utilization rate; having good stability and being able to maintain activity in a saline-alkali environment; being able to form a complementary and synergistic effect with plant-derived signal molecules, significantly improving the overall effect of fertilizers.

[0072] 5 Construction of a multi-component synergistic system

[0073] This step is the third core innovation part of the present invention, mainly integrating the above components through processes to construct a multi-component synergistic system, specifically including:

[0074] Humic acid activation: Activate humic acid under the conditions of pH 7.0 - 8.0 and temperature 30 - 40°C for 4 - 6 hours to improve its activity and complexing ability. The activation treatment includes: adjusting the molecular structure of humic acid (by adding an oxidant H2O2 with a concentration of 0.5 - 1.0% and controlling the oxidation time for 30 - 60 minutes) to increase the number and activity of its active groups; adjusting the molecular weight distribution of humic acid (by ultrasonic treatment with a power of 400 - 600W and a treatment time of 15 - 30 minutes) to improve its binding ability with signal molecules and amino acids; improving the stability and persistence of humic acid in saline - alkali soil (by adding Ca 2+ and Mg 2+ ions with a concentration of 0.2 - 0.5%). The activated humic acid can more effectively improve the saline - alkali soil structure, promote the reproduction of beneficial microorganisms, and increase soil fertility.

[0075] Seaweed extract integration: Compound the seaweed extract (mainly containing components such as alginate and polysaccharides) with humic acid through a process to form a seaweed - humic acid complex. The compounding process includes: adjusting the pH value to 6.5 - 7.5 and the ionic strength (adding NaCl with a concentration of 0.1 - 0.3mol / L) to promote the combination between the two; adding a bridging agent (polyvalent metal ions such as Ca 2+ and Fe 3+ with a concentration of 0.05 - 0.1mol / L, or chitosan, carboxymethyl cellulose, etc., with an addition amount of 1 - 2% of the mixture) to enhance the binding force between the two; making the two fully mixed by gentle stirring (150 - 250r / min) for 4 - 6 hours to form a uniform complex. This complex has multiple functions such as improving soil structure, enhancing water and fertilizer retention capacity, and promoting microbial activity.

[0076] Construction of signal molecule - humic acid composite regulatory network: Integrate the activated plant - derived signal molecules with the humic acid - seaweed complex through an integration process to construct a signal molecule - humic acid composite regulatory network. The integration process includes: making the signal molecules evenly distributed in the humic acid - seaweed complex by physical methods (ultrasonic treatment with a power of 300 - 400W and a treatment time of 10 - 15 minutes, or high - pressure homogenization with a pressure of 20 - 30MPa and a passing number of 3 - 5 times); adjusting the pH value to 6.0 - 7.0 and the temperature to 25 - 30°C to promote the interaction between the signal molecules and humic acid; adding a stabilizer (antioxidants such as vitamin C and vitamin E with an addition amount of 0.1 - 0.3%) to improve the stability of the composite system. This composite regulatory network can achieve the directional release of signal molecules and significantly improve their bioavailability.

[0077] Integration of Carrier System and Active Ingredients: Integrate the nanoscale carrier system with the signal molecule-humic acid composite regulation network and the stress-resistant amino acid complex to construct the final multi-component synergistic system. The integration process includes: uniformly mixing each component through a mixing technique (programmable temperature control mixing, with the temperature gradually rising from 10°C to 30°C over 2 - 3 hours; or multi-stage emulsification, with a high-speed stage at 8000 - 10000 r / min for 10 - 15 minutes and a low-speed stage at 1000 - 2000 r / min for 30 - 60 minutes); adjusting the pH value to 6.5 - 7.0, the viscosity to 50 - 100 mPa·s, and the surface tension to 30 - 40 mN / m to ensure the compatibility and stability among components; and bringing the whole system to the optimal state through a mature process (storing at a low temperature of 4 - 8°C in the dark). This multi-component synergistic system can achieve the synergistic effect among components and significantly improve the overall effect of fertilizers.

[0078] Final Processing of the Product: According to the actual application requirements, conduct the final processing on the above multi-component synergistic system to produce finished fertilizers. The processing methods include: liquid fertilizers (direct packaging), concentrated liquid fertilizers (through low-temperature concentration technology, with the temperature not exceeding 45°C and concentrated to 60 - 70% of the original volume), water-soluble powders (through spray drying technology, with an inlet temperature of 120 - 150°C and an outlet temperature of 70 - 80°C), etc., to meet the requirements of different application methods.

[0079] The multi-component synergistic system constructed by the above steps has the following characteristics: a synergistic effect is formed among components, and the overall effect far exceeds that of single components; the carrier system can carry active ingredients through saline-alkali soil and reach the vicinity of plant roots; the signal molecule can trigger the expression of the plant's own stress-resistant genes and activate the plant's stress-resistant response from within; the stress-resistant amino acids can provide the nutrients required by plants from the outside, forming an internal and external synergistic effect; humic acid and seaweed extracts can improve the saline-alkali soil environment and create more favorable conditions for plant growth.

[0080] Experimental Examples

[0081] Experiment 1: Test on the Penetration Ability of the Carrier System

[0082] 1. Experimental Purpose

[0083] Verify the penetration ability of the soil penetration carrier system constructed in the present invention in saline-alkali soil and the transfer effect on active ingredients.

[0084] 2. Experimental Materials

[0085] The nanoscale carrier system prepared in the present invention (experimental group);

[0086] Traditional amino acid water-soluble fertilizer (control group A);

[0087] Commercially available water-soluble fertilizer for saline-alkali soil (control group B);

[0088] Saline-alkali soil sample (pH 9.5, salt content 0.5%);

[0089] Fluorescent marker (Rhodamine B) is used to label the active ingredient;

[0090] Soil column (inner diameter 10 cm, height 40 cm, layered sampling device).

[0091] 3. Experimental methods

[0092] Soil column filling: The collected saline-alkali soil is fully air-dried, ground, passed through a 2 mm sieve, and then evenly filled into the soil column and compacted to the natural bulk density state (about 1.3 g / cm 3 );

[0093] Sample preparation: The fluorescent marker is added to three groups of fertilizer samples respectively, and the concentration of the fluorescent substance is 50 mg / L.

[0094] Penetration test:

[0095] The three groups of labeled fertilizer samples are respectively dropped onto the surface of different soil columns, and the addition amount is 50 mL / column;

[0096] Place at room temperature (25 ± 2 °C) for 24 hours;

[0097] Layered sampling: Take 10 g of soil samples from 0 - 5 cm, 5 - 10 cm, 10 - 15 cm, 15 - 20 cm, 20 - 25 cm, 25 - 30 cm, and 30 - 35 cm of the soil column respectively;

[0098] Extract the fluorescent marker in the sample: Extract with 50 mL of extraction solution (ethanol:water = 7:3) on an oscillator for 2 hours (150 r / min);

[0099] Determine the content of the fluorescent marker in each layer of the extraction solution by a fluorescence spectrophotometer (excitation wavelength 530 nm, emission wavelength 590 nm);

[0100] Calculate the penetration depth and distribution: Take the maximum depth with the fluorescence intensity greater than 5% of the background value as the effective penetration depth index.

[0101] 4. Experimental results

[0102] The penetration depth distribution of different fertilizer samples in saline-alkali soil is shown in Table 1:

[0103] Table 1 Penetration distribution of different fertilizer samples in saline-alkali soil (fluorescence intensity value, relative unit)

[0104] Soil depth (cm) The carrier system of the present invention Traditional amino acid fertilizer Commercially available special fertilizer for saline-alkali land 0-5 85.6±3.2 92.3±4.1 88.9±3.8 5-10 76.2±2.9 43.5±2.6 56.7±2.5 10-15 68.4±3.5 12.7±1.8 29.4±2.2 15-20 52.7±2.3 4.3±0.6 10.8±1.5 20-25 38.5±2.0 1.2±0.3 3.6±0.8 25-30 23.8±1.7 0.5±0.2 1.4±0.4 30-35 5.6±0.8 0.3±0.1 0.5±0.2

[0105] Comparison of effective penetration depths is shown in Figure 1 .

[0106] The experimental results show that the carrier system of the present invention exhibits significant penetration ability in saline-alkali soil. According to the fluorescence intensity distribution data, the effective penetration depth of the carrier system of the present invention can reach 28 - 30 cm, which is much higher than that of traditional amino acid fertilizers (5 - 8 cm) and commercially available special fertilizers for saline-alkali land (12 - 15 cm). In the soil layer of 10 - 25 cm, the content of active ingredients in the carrier system of the present invention is 5 - 30 times that of traditional amino acid fertilizers and 2 - 10 times that of commercially available special fertilizers for saline-alkali land. This result proves that the nano-scale carrier system constructed by the present invention can effectively overcome the fixation of fertilizer active ingredients by saline-alkali soil, significantly improve the distribution of active ingredients in deep soil, and provide guarantee for the absorption of nutrients by plant roots in deep soil.

[0107] Experiment 2: Evaluation of the activation of stress resistance response by plant-derived signal molecules

[0108] 1. Experimental purpose

[0109] Evaluate the activation effect of the plant-derived signal molecules prepared by the present invention on the saline-alkali resistance response of crops and the improvement of their saline-alkali resistance ability.

[0110] 2. Experimental materials

[0111] Experimental plants: Wheat (variety: Jimai 22, saline-alkali sensitive type).

[0112] Experimental treatments:

[0113] Treatment A: Amino acid water-soluble fertilizer containing plant-derived signal molecules prepared by the present invention;

[0114] Treatment B: Amino acid water-soluble fertilizer without plant-derived signal molecules (other components are the same as A);

[0115] Treatment C: Commercially available special fertilizer for saline-alkali land;

[0116] Control group: Clear water;

[0117] Saline-alkali stress conditions: Use a mixed solution of NaCl and Na2CO3 (molar ratio of 9:1) to prepare stress solutions with three saline-alkali concentration gradients of 0.3%, 0.5% and 0.8% to simulate different degrees of saline-alkali stress.

[0118] 3. Experimental method

[0119] Plant cultivation:

[0120] Select wheat seeds of the same size, disinfect them in 0.1% NaClO solution for 15 minutes, and then rinse them 5 times with deionized water;

[0121] The seeds were placed on moist filter paper and germinated at 25°C in the dark for 48 hours;

[0122] The seeds with uniform germination were transferred to plastic pots (15 cm in diameter and 12 cm in height) containing vermiculite, with 30 seeds per pot;

[0123] They were cultivated in a controlled environment chamber with a light / dark cycle of 16 / 8 hours, a light intensity of 350 μmol·m -2 ·s -1 , a temperature of 23 ± 2°C, and a relative humidity of 65 ± 5%;

[0124] They were watered with 1 / 2 Hoagland nutrient solution, and the nutrient solution was changed every 3 days;

[0125] Treatment application:

[0126] When the seedlings grew to the three-leaf stage, different fertilizers were applied to the corresponding treatment groups at a concentration of 300-fold diluted solution, and 100 mL was applied to each pot;

[0127] Three days after fertilization, saline-alkali stress treatment was started, and saline-alkali solutions with three concentration gradients of 0.3%, 0.5%, and 0.8% were applied to each group;

[0128] The stress treatment lasted for 21 days, and the corresponding fertilizers were applied once every 3 days during this period;

[0129] Index determination:

[0130] Morphological indexes: Parameters such as plant height, root length, aboveground and underground fresh weight, and dry weight were measured on the 7th, 14th, and 21st days after stress treatment;

[0131] Physiological indexes: Plant leaves were collected to measure the proline content (ninhydrin method), soluble sugar content (anthrone method), malondialdehyde content (TBA method), and SOD and POD activities (spectrophotometry);

[0132] Gene expression: Plant leaves were collected, total RNA was extracted, and real-time fluorescence quantitative PCR analysis was performed to detect the changes in the expression levels of key genes related to saline-alkali resistance (NHX1, SOS1, P5CS, DREB2A);

[0133] Mineral element content: The Na content in the aboveground part of the plant was measured + , K + content and the K + / Na + ratio (atomic absorption spectrophotometry);

[0134] 4. Experimental results

[0135] The survival rates of wheat under different treatments under 0.5% saline-alkali stress are shown in Table 2:

[0136] Table 2 Effects of different treatments on the survival rate of wheat under saline-alkali stress (%)

[0137] Stress time (days) Treatment A Treatment B Treatment C Control group 7 97.8±2.1 92.3±3.2 85.6±4.1 76.7±5.3 14 92.5±3.5 78.9±4.5 67.2±5.2 45.3±6.2 21 88.7±4.2 60.4±5.6 52.8±4.9 28.6±5.8

[0138] The growth parameters of wheat after 21 days of 0.5% saline-alkali stress under different treatments are shown in Table 3:

[0139] Table 3 Effects of different treatments on the growth parameters of wheat under saline-alkali stress

[0140] Growth parameters Treatment A Treatment B Treatment C Control group Plant height (cm) 32.6±2.3 25.7±2.1 22.4±1.8 15.8±1.5 Root length (cm) 18.9±1.5 12.4±1.3 10.8±1.1 7.5±0.9 Shoot fresh weight (g / plant) 5.87±0.42 3.56±0.31 2.95±0.28 1.63±0.22 Root fresh weight (g / plant) 2.43±0.25 1.52±0.18 1.25±0.15 0.78±0.12 Shoot dry weight (g / plant) 0.96±0.08 0.58±0.07 0.48±0.06 0.27±0.05 Root dry weight (g / plant) 0.41±0.05 0.25±0.04 0.21±0.03 0.13±0.03

[0141] The expression levels of salt-tolerance related genes are shown in Figure 2 ;

[0142] After 21 days of 0.5% saline-alkali stress, the Na + , K + contents and the K + / Na + ratio in wheat are shown in Table 4:

[0143] Table 4 Effects of different treatments on the ion balance in wheat under saline-alkali stress

[0144] Ion index Treatment A Treatment B Treatment C Control group <![CDATA[Sodium + Content (mg / g DW)]]> 8.5±0.7 14.6±1.2 16.9±1.4 22.3±1.8 <![CDATA[K + Content (mg / g DW)]]> 32.7±2.5 25.3±2.1 22.8±1.9 18.5±1.6 <![CDATA[K + / Na + Ratio]]> 3.85±0.31 1.73±0.18 1.35±0.15 0.83±0.09

[0145] The comparison of the growth conditions of wheat under different concentrations of saline-alkali stress is shown in Figure 3 .

[0146] The experimental results show that the amino acid water-soluble fertilizer containing plant-derived signal molecules prepared by the present invention (Treatment A) has a significant promoting effect on the salt tolerance of wheat. After 21 days of 0.5% saline-alkali stress, the survival rate of wheat in the Treatment A group reached 88.7%, which was 28.3 percentage points higher than that of the amino acid water-soluble fertilizer without plant-derived signal molecules (Treatment B), 35.9 percentage points higher than that of the commercially available special fertilizer for saline-alkali land (Treatment C), and 60.1 percentage points higher than that of the control group.

[0147] In terms of growth parameters, the indexes such as plant height, root length, and biomass of wheat in the Treatment A group were significantly higher than those of other treatment groups. Especially in terms of root system development, the root length of the Treatment A group was 52.4% and 75.0% higher than that of the Treatment B group and the Treatment C group respectively, indicating that the plant-derived signal molecules significantly promoted the growth and development of the root system under saline-alkali stress.

[0148] Gene expression analysis further confirmed the activation effect of plant-derived signal molecules on the plant's own stress resistance response. The expression levels of salt and alkali resistance-related genes (NHX1, SOS1, P5CS, DREB2A) in treatment group A were significantly up-regulated, being on average 2-3 times higher than those in treatment group B and treatment group C. Among them, the expression level of the PSCS gene (the key enzyme gene for proline synthesis) was the highest, reaching 9.6 times that of the control group, which was consistent with the higher proline content in the wheat plants of treatment group A, indicating that plant-derived signal molecules can effectively activate the osmotic regulation mechanism in plants.

[0149] Ion balance analysis showed that the Na + content in the wheat plants of treatment group A was significantly reduced, while the K + content was significantly increased. The K + / Na + ratio reached 3.85, which was 4.6 times that of the control group. This indicates that plant-derived signal molecules can activate the ion balance regulation mechanism, effectively reduce the accumulation of Na + in plants, and promote the absorption of K + , thus significantly improving the survival ability of plants in saline-alkali environments.

[0150] The experimental results of different concentrations of saline-alkali stress showed that with the increase of saline-alkali concentration, the growth of wheat in each treatment group was significantly inhibited, but the inhibition degree of treatment group A was the smallest. Under high-concentration saline-alkali conditions (0.8%), the relative growth rate of treatment group A still reached 53.8%, which was 26.5, 33.2 and 44.4 percentage points higher than those of treatment group B, treatment group C and the control group respectively, indicating that the plant-derived signal molecules of the present invention have a significant effect on enhancing the ability of plants to resist high-concentration saline-alkali stress.

[0151] In summary, the experimental results of this study proved that the plant-derived signal molecules in the present invention can significantly activate the plant's own salt and alkali resistance response, thereby comprehensively improving the plant's salt and alkali resistance ability. The plant-derived signal molecules and the exogenously added stress-resistant amino acids form an internal and external synergistic effect, which not only significantly improves the survival rate of plants under saline-alkali conditions, but also promotes the growth and development of plants, enabling plants to better cope with saline-alkali stress.

[0152] Experiment 3: Evaluation of the Stability and Long-Term Effectiveness of Fertilizers in Saline-Alkali Soils

[0153] 1. Experimental Purpose

[0154] Evaluate the stability and long-term effectiveness of the amino acid water-soluble fertilizer prepared in the present invention in saline-alkali soils, and verify the functionality of the slow-release system.

[0155] 2. Experimental Materials

[0156] Experimental soil: Collected from a typical saline-alkali land in Bayannao'er City, Inner Mongolia Autonomous Region (pH 8.7, conductivity 5.4 mS / cm, organic matter content 0.87%).

[0157] Experimental samples:

[0158] Sample A: Amino acid water-soluble fertilizer containing a slow-release system prepared by the present invention;

[0159] Sample B: Amino acid water-soluble fertilizer without a slow-release system (other components are the same as those of A);

[0160] Sample C: A commercially available slow-release fertilizer specifically for saline-alkali land;

[0161] Analytical instruments:

[0162] High-performance liquid chromatograph (Agilent 1260 Infinity II);

[0163] Ion chromatograph (Thermo Scientific Dionex ICS-5000+);

[0164] Atomic absorption spectrophotometer (Analytik Jena contrAA 800);

[0165] 3. Experimental methods

[0166] Degradation kinetics study:

[0167] Prepare a series of saline-alkali soils added with different experimental samples, and maintain the same moisture content (60% of field water holding capacity) and temperature (25 ± 2 °C);

[0168] Sample at 0, 7, 14, 21, 28, 42, 56, 70, 84, 98, and 112 days respectively, and extract the active components of the fertilizer in the soil;

[0169] Determine the change in amino acid content by high-performance liquid chromatography, and analyze the degradation curves of different samples;

[0170] Establish a first-order kinetic equation and calculate the half-life of different samples;

[0171] Release kinetics study:

[0172] Use a modified soil column leaching device to add the same amount of experimental samples to the top of the saline-alkali soil column;

[0173] Simulate precipitation conditions (designed according to the local annual average precipitation), and perform leaching operations every 7 days;

[0174] Collect the leachate and determine the contents of active components such as amino acids, nitrogen, phosphorus, and potassium in the leachate;

[0175] Draw the cumulative release curves and release rate curves of different samples;

[0176] Evaluation of fertilizer effect duration:

[0177] In pot conditions, wheat was used as an indicator crop;

[0178] Apply equal amounts of experimental samples at one time and measure soil and plant samples regularly;

[0179] Monitor wheat growth and changes in soil nutrient content;

[0180] Sampling was carried out every 14 days, and observation was continued for 112 days;

[0181] 4. Experimental Results

[0182] The degradation kinetic curves of different samples in saline-alkali soil are shown in Figure 4 ;

[0183] Cumulative nutrient release curves of different samples under simulated precipitation conditions are shown in Figure 5 ;

[0184] Nutrient release rate curves of different samples are shown in Figure 6 ;

[0185] The temporal trends of available nitrogen content in the soil after application of different samples are shown in Table 5;

[0186] Table 5 Time changes of available nitrogen content in soil after application of different samples (mg / kg)

[0187] Time (days) Sample A Sample B Sample C Blank control 0 45.3±3.1 45.8±3.2 45.1±3.0 42.5±2.8 14 78.6±5.2 125.7±8.3 85.4±5.6 41.8±2.9 28 82.5±5.4 86.2±5.7 92.1±6.0 42.3±3.0 42 85.4±5.8 61.5±4.1 88.3±5.9 41.7±2.8 56 79.8±5.3 49.7±3.3 76.5±5.0 40.9±2.7 70 77.3±5.2 47.2±3.1 68.2±4.5 41.2±2.7 84 73.5±4.9 46.5±3.2 59.3±3.9 40.6±2.8 98 68.9±4.5 45.3±3.0 54.7±3.6 41.0±2.7 112 65.2±4.3 44.1±2.9 51.2±3.4 40.8±2.7

[0188] Comparison of the fertilizer effect persistence of different samples during the wheat growth period Figure 7 .

[0189] Experimental results show that the amino acid water-soluble fertilizer containing a slow-release system (Sample A) prepared by the present invention exhibits remarkable stability and long-lasting effectiveness in saline-alkali soils. Based on the degradation kinetics, Sample A has a half-life of 112.5 days in saline-alkali soils, which is much longer than that of an amino acid water-soluble fertilizer without a slow-release system (Sample B, with a half-life of 20.1 days) and a commercially available slow-release fertilizer specifically for saline-alkali soils (Sample C, with a half-life of 61.8 days).

[0190] The nutrient release curve of Sample A presented an ideal S-shaped curve. During the 112-day observation period, the cumulative release rate reached 84.3%. While for Sample B, more than 58% of the nutrients were released within the first 28 days, and the cumulative release rate approached 100% by the end of the experiment. This indicates that the nutrient release of Sample B was too fast, which was likely to cause nutrient waste in the early stage and nutrient deficiency in the later stage. In contrast, the nutrient release rate of Sample A remained relatively stable throughout the observation period, with the weekly release rate maintained between 4.5% and 5.8%, which was very close to the ideal uniform release mode (about 5.7% / week).

[0191] The monitoring results of soil available nitrogen content further verified the slow-release effect of Sample A. After applying Sample B, the soil available nitrogen content reached the peak (125.7 mg / kg) on the 14th day, and then decreased rapidly. By the 56th day, it was close to the initial level. While for the soil treated with Sample A, the soil available nitrogen content remained at a relatively high level (78.6 - 85.4 mg / kg) from the 14th day to the 56th day, and then decreased slowly. At the end of the experiment (the 112th day), it still remained at 65.2 mg / kg, significantly higher than the initial level.

[0192] The wheat growth curve showed that in the middle and late growth stages (56 - 112 days), the growth potential of wheat in the treatment group with Sample A was significantly better than that in the treatment groups with Sample B and Sample C. By the 112th day, the wheat biomass index in the treatment group with Sample A reached 503, which was 77% higher than that of Sample B, 27.3% higher than that of Sample C, and 169% higher than that of the control group. This indicates that Sample A can provide a continuous and stable nutrient supply for crops, especially in the key middle and late growth stages of crops.

[0193] In summary, the experimental results of this study demonstrated that the slow-release system constructed in this invention could significantly improve the stability and long-term effectiveness of amino acid water-soluble fertilizers in saline-alkali soils, achieving slow nutrient release and long-term supply, providing continuous and stable nutrition for crops throughout the growth period, and avoiding the disadvantages of traditional amino acid water-soluble fertilizers such as fast nutrient release and short long-term effectiveness.

[0194] The above described the embodiments of the present invention. However, these embodiments are not limited to the above specific implementation manners. The above specific implementation manners are merely illustrative rather than restrictive. Under the inspiration of this embodiment, those of ordinary skill in the art can also make more equivalent embodiments in various forms, all of which fall within the protection scope of this embodiment.

Claims

1. A water-soluble amino acid-containing fertilizer, characterized in that, It comprises raw materials in the following parts by weight: 15 - 25 parts of stress - resistance related amino acids, 8 - 15 parts of salt - tolerant plant raw materials, 10 - 15 parts of humic acid, 8 - 12 parts of seaweed extract, 5 - 8 parts of medium - chain fatty acids, 2 - 4 parts of organosilicon surfactant, and 2 - 3 parts of biogenic wetting agent.

2. The amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The stress - resistance related amino acids include L - proline, betaine, glutamic acid and glycine, which are mixed according to the mass ratio of 3:3:2:

2.

3. The amino acid-containing water-soluble fertilizer according to claim 1, wherein The salt - tolerant plant raw materials are selected from Salicornia europaea or Suaeda glauca growing in high - salinity environments.

4. A preparation method of an amino acid-containing water-soluble fertilizer, characterized in that, It includes the following steps: Step 1: Obtain raw materials according to the ratio. Step 2: Construct a soil - penetrating carrier system, including: activation of medium - chain fatty acids, construction of amphiphilic carriers, integration of biogenic wetting agents and sol - gel transformation, to form a nanoscale dispersion with a particle size of 50 - 200 nm. Step 3: Extract and activate plant - derived signal molecules from salt - tolerant plant raw materials. Step 4: Prepare stress - resistance amino acid complexes. Step 5: Compound humic acid with seaweed extract, and then integrate it with plant - derived signal molecules, soil - penetrating carrier systems and stress - resistance amino acid complexes to construct a multi - component synergistic system.

5. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The step of activating medium - chain fatty acids includes: carrying out an esterification reaction on medium - chain fatty acids and alcohols at a molar ratio of 1:1.2 - 1.5 at 55 - 65 °C for 4 - 6 hours to generate an amphiphilic molecular precursor with an HLB value of 8 - 12.

6. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The step of constructing amphiphilic carriers includes: compounding the activated medium - chain fatty acids with organosilicon surfactant at a mass ratio of 1:0.3 - 0.5 at 40 - 50 °C, and treating it with high - shear emulsification technology at 8000 - 10000 r / min for 15 - 25 minutes.

7. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The step of sol - gel transformation includes: adding polyvalent metal ions or natural polysaccharides as cross - linkers for gelation under the conditions of pH 5.5 - 6.5 and temperature 25 - 35 °C.

8. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The extraction step of plant - derived signal molecules includes: adding a water - ethanol mixed solvent at a solid - liquid ratio of 1:8 - 10, and extracting for 4 - 6 hours using a bionic extraction technology at 35 - 45 °C, where the pH value is controlled at 5.8 - 6.2 and the redox potential is controlled at 150 - 200 mV.

9. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, characterized in that, The activation step of plant - derived signal molecules includes: adding amino acids to form hydrogen - bond complexes, carrying out hydroxymethylation or carboxymethylation modification on signal molecules, and protecting them by cyclodextrin inclusion or polysaccharide embedding.

10. The preparation method of an amino acid-containing water-soluble fertilizer according to claim 1, wherein, Carry out amino acid activation treatment at pH 6.0 - 7.0 and temperature 30 - 40 °C for 2 - 3 hours; add trace element chelates to form amino acid - trace element complexes; add stabilizers and adjust the pH value to 6.0 - 6.5.

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