Vibration slicing device capable of correcting knife shape and used for large-size biological sample

Abstract

The invention relates to the technical field of biological sample slicing equipment, in particular to a vibration slicing device capable of correcting a knife type for a large-size biological sample. According to the technical scheme, the vibration slicing device comprises a vibration slicing device body for cutting a sample, and further comprises a driving mechanism, a transmission mechanism and a cutting mechanism which are arranged on the vibration slicing device body. Through the design of the driving mechanism, the transmission mechanism, the cutting mechanism and other structures, when the device is used, small-size samples such as mouse brains and large-size samples such as monkey brains can obtain the same slicing quality. The stepped plate spring mechanism additionally arranged on the device can effectively regulate and control the inherent frequency of vibration. The bow-shaped plate spring flexible mechanism is composed of a middle plate spring flexible mechanism and an inner side plate spring flexible mechanism, interference of vibration terminal connection on amplitude can be reduced, parasitic motion is stabilized, and the problem that parasitic motion at the two ends of the blade is too large is solved. According to the blocking flexible clamping mechanism, the knife type is quantitatively changed through deformation, mutual influence of all flexible blocks is small, the knife type can be accurately adjusted, and various slicing requirements are met.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample slicing equipment, and particularly to a vibration slicing device with a correctable blade type for large-volume biological samples. Background Art

[0002] In the field of modern biomedicine, due to the inherent structural characteristics of biological tissues, it is often necessary to cut the tissues into thin slices with a uniform thickness ranging from several micrometers to dozens of micrometers during research. Then, through steps such as optical clearing, staining, and imaging, research results can be obtained according to different requirements. Slicing is essential throughout this entire process. Currently, there are different forms of slicing techniques. Among them, vibration slicing is particularly suitable for slicing embedded soft tissues, which can preserve more properties of biological samples and facilitate the acquisition of more biological information. Vibration slicing mainly utilizes the mechanism of driving a blade to reciprocate to achieve the fracture of soft tissues. If combined with a high-precision displacement platform, continuous slicing of biological tissues with a uniform thickness ranging from dozens to hundreds of micrometers can be achieved. According to the implementation form of vibration, vibration slicing can be divided into different types, but their purpose is to reduce parasitic motion, improve the slicing quality, and thereby improve the continuity and integrity of the acquired data.

[0003] There are several types of vibration slicing devices according to the vibration form. A slicing machine described in Chinese Patent CN101500767 uses an eccentric wheel combined with a guide rail structure to achieve reciprocating vibration. By changing the motor speed, frequency conversion operation can be achieved. Therefore, it is not applicable to high-frequency vibration, and at the same time, it does not limit the parasitic motion of the cutting edge, and is only suitable for small-volume samples and low-frequency slicing; U.S. Patent US6651538 describes a vibration slicing device driven by electromagnetic force and towed by a spring to achieve reciprocating motion. Similarly, it does not limit the parasitic motion. The phenomenon that the parasitic motion in the middle of the clamped blade is small and the parasitic motion at both ends is large will occur along the vibration direction; Chinese Patent CN104729874 describes a vibration slicing machine driven by a variable electromagnetic force, which uses a double leaf spring flexible mechanism to limit the parasitic motion, and at the same time, the tool inclination adjustment is achieved through a fine-tuning nut. However, the vibration frequency is limited, and the change of the blade type is not considered. Since it uses a leaf spring flexible mechanism with one end fixed and the other end vibrating, the parasitic motion at both ends of the tool is large and it is not suitable for slicing large-volume samples; Chinese Patent CN111504685 describes a vibration slicing device that uses a voice coil motor to drive, a flexible mechanism to restrain and correct the change of parasitic motion. Among them, the use of a double parallelogram mechanism will cause the parasitic motion at both ends of the tool to be too large. At the same time, it has high requirements for processing accuracy. If the centroid of the vibrating part is not in the vibration direction, the parasitic motion will be increased, and the influence of the change of the blade type on the flatness of the slicing surface is not considered. Generally speaking, the existing vibration slicing devices have different degrees of problems in terms of vibration frequency, parasitic motion control, applicable sample volume, consideration of blade type change, and processing accuracy. Summary of the Invention

[0004] The object of the present invention is to propose a vibration slicing device with a modifiable knife shape for large-volume biological samples, in view of the problems to varying degrees existing in the existing vibration slicing devices in terms of vibration frequency, parasitic motion control, applicable sample volume, consideration of knife shape changes, and processing accuracy.

[0005] The technical solution of the present invention: A vibration slicing device with a modifiable knife shape for large-volume biological samples, including a vibration slicing device body for cutting samples, further including: a driving mechanism, a transmission mechanism, and a cutting mechanism provided on the vibration slicing device body; the driving mechanism includes a voice coil motor, a fixing member, and a constraining member; the transmission mechanism includes a first outer stepped leaf spring flexible mechanism, a second outer stepped leaf spring flexible mechanism, a pair of middle leaf spring flexible mechanisms, a middle inner leaf spring connection block, a pair of inner leaf spring flexible mechanisms, a fixing bracket of the leaf spring, and a vibration terminal; the cutting mechanism includes a tool holder connected to the vibration terminal, a blade clamping mechanism, and a blade.

[0006] Optionally, the fixing member is connected to the threaded hole on the fixing bracket through a bolt, the diameter of the waist-shaped groove of the fixing member is 1 mm larger than the diameter of the bolt, the voice coil motor realizes reciprocating motion with the assistance of an external cylindrical magnet, the distance between the internal coil and the external cylindrical magnet is adjusted by manually moving the fixing member through the waist-shaped groove of the fixing member, and the internal coil and the constraining member are connected through bolts to jointly drive the vibration terminal to vibrate.

[0007] Optionally, one end of the first outer stepped leaf spring flexible mechanism is connected to the fixing bracket of the leaf spring, one end of the second outer stepped leaf spring flexible mechanism is connected to the vibration terminal, one end of the middle leaf spring flexible mechanism is connected to the vibration terminal, and the other end is connected to the middle inner leaf spring connection block. The other end of the middle inner leaf spring connection block is connected to the inner leaf spring flexible mechanism, and the other end of the inner leaf spring flexible mechanism is connected to the fixing bracket of the leaf spring. A pair of square holes are provided on the vibration terminal.

[0008] Optionally, the vibration terminal drives the blade clamping mechanism and the blade to realize reciprocating linear motion through the tool holder. The tool holder is connected to the vibration terminal through a threaded hole, the blade clamping mechanism is connected to the tool holder through a thread pair, and the thread pair of the tool holder is set as a triangular structure.

[0009] Optionally, a gasket for adjusting the rotation angle of the blade is provided between the vibration terminal and the tool holder, and the rotation angle of the blade is adjusted through the gasket at the contact surface position of the tool holder and the blade clamping mechanism.

[0010] Optionally, the blade clamping mechanism includes evenly distributed clamping pieces, and fine-tuning bolts that can be tightened or loosened at corresponding positions are provided at the convex or concave positions of the blade.

[0011] Optionally, the voice coil motor on the driving mechanism changes its working state by adjusting the relative position between the internal coil and the external cylindrical magnet.

[0012] Optionally, the first outer stepped leaf spring flexible mechanism and the second outer stepped leaf spring flexible mechanism of the transmission mechanism are used to balance the vibration frequency and amplitude of the transmission mechanism, and the middle leaf spring flexible mechanism, the middle inner leaf spring connecting block, the inner leaf spring flexible mechanism, the first outer stepped leaf spring flexible mechanism and the second outer stepped leaf spring flexible mechanism are used to control the linear reciprocating motion of the vibrating terminal.

[0013] Optionally, the cutting mechanism adjusts the slicing state by adjusting the blade.

[0014] In summary, the present application includes at least one of the following beneficial technical effects:

[0015] Through the design of structures such as the driving mechanism, the transmission mechanism and the cutting mechanism, when using this device, small-volume samples such as mouse brains and large-volume samples such as monkey brains can obtain the same slicing quality. The added stepped leaf spring mechanism in the device can effectively regulate the vibration natural frequency. The bow-shaped leaf spring flexible mechanism is composed of the middle and inner leaf spring flexible mechanisms, which can reduce the interference of the connection of the vibrating terminal on the amplitude, stabilize the parasitic motion, and overcome the problem of excessive parasitic motion at both ends of the blade. The segmented flexible clamping mechanism changes the blade shape quantitatively through deformation, and the flexible blocks have little influence on each other, so the blade shape can be accurately adjusted to meet various slicing requirements. Description of the Drawings

[0016] Figure 1 Provide a structural schematic diagram of a vibration slicing device with a modifiable blade shape for large-volume biological samples of the present invention;

[0017] Figure 2 It is a schematic diagram of the inconsistent motion states of the blade and the tool holder;

[0018] Figure 3 It is a schematic diagram of the changes in different states of the blade cutting the sample;

[0019] Figure 4 For Figure 1 The connection cross-sectional view of the fixing part and the voice coil motor in

[0020] Figure 5 It is a structural schematic diagram of the blade clamping mechanism;

[0021] Figure 6 It is a schematic diagram of the change of the blade clamping mechanism.

[0022] Reference numerals: 1. Second outer stepped leaf spring flexible mechanism; 2. Tool rest; 3. Thread pair; 4. Blade clamping mechanism; 5. Clip; 6. Blade; 7. Square hole; 8. Threaded hole; 9. Middle leaf spring flexible mechanism; 10. Inner leaf spring flexible mechanism; 11. First outer stepped leaf spring flexible mechanism; 12. Voice coil motor; 1201. External cylindrical magnet; 1202. Internal coil; 13. Vibration terminal; 14. Fixing member; 15. Fixed bracket; 16. Constraint member; 17. Middle-inner leaf spring connecting block; 18. Fine adjustment bolt; 19. Sample. Detailed implementation manners

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Apparently, the described embodiments are some but not all of the embodiments of the present invention.

[0024] The components of the embodiments of the present invention usually described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents the selected embodiments of the present invention.

[0025] All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0026] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0027] It should be noted that the term "comprising", "including" or any other variant thereof is intended to cover a non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0028] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0029] Embodiment

[0030] As Figures 1 to 6 shown, a vibration sectioning device with a modifiable blade shape for large-volume biological samples proposed by the present invention includes a vibration sectioning device body for cutting a sample 19, a driving mechanism, a transmission mechanism, and a cutting mechanism provided on the vibration sectioning device body. The cutting mechanism adjusts the sectioning state by adjusting a blade 6. This device operates in cooperation with the driving, transmission, and cutting mechanisms. The voice coil motor 12, fixing member 14, and restraint member 16 in the driving mechanism work by adjusting the relative position between the internal coil 1202 and the external cylindrical magnet 1201. The transmission mechanism includes, for example, the first and second outer stepped leaf spring flexible mechanisms, etc. Using a multi-layer leaf spring structure, especially the stepped leaf spring, one end is fixed and the other end vibrates, balancing and adjusting the natural frequency and amplitude, overcoming the unstable output of the voice coil motor, stabilizing the parasitic movement of the blade, and reducing the influence of the blade size on the sectioning quality. The cutting mechanism includes a tool holder 2, a blade clamping mechanism 4, and a blade 6. The clamping pieces 5 of the blade clamping mechanism are evenly distributed. By using the fine-tuning bolts 18 to correct the deformation of the blade 6, the specified area is corrected in blocks, restricting the influence of the mismatch between the blade shape and the vibration direction and the change of the blade shape on the sectioning flatness, and finally constructing a stable device suitable for vibration sectioning of large-volume biological samples.

[0031] In addition, as Figures 1 to 6 shown, the driving mechanism includes a voice coil motor 12, a fixing member 14, and a restraint member 16; the transmission mechanism includes a first outer stepped leaf spring flexible mechanism 11, a second outer stepped leaf spring flexible mechanism 1, a pair of middle leaf spring flexible mechanisms 9, a middle-inner leaf spring connection block 17, a pair of inner leaf spring flexible mechanisms 10, a fixing bracket 15 of the leaf spring, and a vibration terminal 13; the cutting mechanism includes a tool holder 2, a blade clamping mechanism 4, and a blade 6 connected to the vibration terminal 13. The blade clamping mechanism 4 includes evenly distributed clamping pieces 5. Fine-tuning bolts 18 that can be tightened or loosened at corresponding positions are provided at the convex or concave positions of the blade 6. The voice coil motor 12 on the driving mechanism changes its working state by adjusting the relative position between the internal coil 1202 and the external cylindrical magnet 1201.

[0032] Among them, as Figures 1 to 2As shown, the fixing member 14 is connected to the threaded hole on the fixing bracket 15 by bolts. The diameter of the waist-shaped slot of the fixing member 14 is 1 mm larger than the diameter of the bolts. The voice coil motor 12 realizes reciprocating motion with the assistance of the external cylindrical magnet 1201. The waist-shaped slot of the fixing member 14 is used to adjust the distance between the internal coil 1202 and the external cylindrical magnet 1201 by manually moving the fixing member 14. Moreover, the internal coil 1202 and the restraint member 16 are connected by bolts to jointly drive the vibration terminal 13 to vibrate.

[0033] Secondly, as Figure 1 shown, one end of the first outer stepped leaf spring flexible mechanism 11 is connected to the fixing bracket 15 of the leaf spring. One end of the second outer stepped leaf spring flexible mechanism 1 is connected to the vibration terminal 13. One end of the middle leaf spring flexible mechanism 9 is connected to the vibration terminal 13, and the other end is connected to the middle inner leaf spring connection block 17. The other end of the middle inner leaf spring connection block 17 is connected to the inner leaf spring flexible mechanism 10. The other end of the inner leaf spring flexible mechanism 10 is connected to the fixing bracket 15 of the leaf spring. A pair of square holes 7 are provided on the vibration terminal 13.

[0034] Furthermore, as Figures 1 to 2 shown, the vibration terminal 13 drives the blade clamping mechanism 4 and the blade 6 to realize reciprocating linear motion through the tool holder 2. The tool holder 2 is connected to the vibration terminal 13 through the threaded hole 8. The blade clamping mechanism 4 is connected to the tool holder 2 through the thread pair 3. Moreover, the thread pair 3 on the tool holder 2 is set as a triangular structure. The thread pair 3 is a connection pair composed of a mutually matching internal thread and external thread. The structural composition is that the external thread is usually located on the surface of a cylinder or a cone and has raised spiral lines; the internal thread is processed on the inner wall of parts such as holes or sleeves and is in the shape of a concave spiral groove. The parameters such as the tooth profile, pitch, and thread angle of the two need to match each other to achieve a tight fit. Its working principle is to rotate the external thread to make it screw with the internal thread, and use the frictional force and mechanical bite generated by the helix angle of the thread to realize the connection, fastening, or transmission function between parts. During the rotation process, the thread pair 3 can convert the rotational motion into a linear motion, or vice versa.

[0035] In addition, as Figure 1 、 Figure 2 and Figure 5 shown, there is a gasket for adjusting the rotation angle of the blade 6 between the vibration terminal 13 and the tool holder 2. The rotation angle of the blade 6 is adjusted by the gasket at the contact surface position between the tool holder 2 and the blade clamping mechanism 4.

[0036] Still further, as Figure 1 and Figure 4As shown, the first outer stepped leaf spring flexible mechanism 11 and the second outer stepped leaf spring flexible mechanism 1 of the transmission mechanism are used to balance the vibration frequency and amplitude of the transmission mechanism. The middle leaf spring flexible mechanism 9, the middle inner leaf spring connecting block 17, the inner leaf spring flexible mechanism 10, the first outer stepped leaf spring flexible mechanism 11, and the second outer stepped leaf spring flexible mechanism 1 are used to control the linear reciprocating motion of the vibrating terminal 13.

[0037] In this embodiment, first, the fixing member 14 is connected to the threaded hole on the fixing bracket 15 through bolts. Since the diameter of the waist-shaped groove of the fixing member 14 is 1 mm larger than the diameter of the bolt, the fixing member 14 can be manually moved to adjust the distance between the inner coil 1202 and the outer cylindrical magnet 1201. The voice coil motor 12 works with the assistance of the outer cylindrical magnet 1201. When the relative positions of the inner coil 1202 and the outer cylindrical magnet 1201 are adjusted, the inner coil 1202 of the voice coil motor 12 can achieve reciprocating motion under the magnetic field of the outer cylindrical magnet 1201. At the same time, the inner coil 1202 and the restraint member 16 are connected by bolts to jointly drive the vibrating terminal 13 to vibrate.

[0038] When the transmission mechanism is used, one end of the first outer stepped leaf spring flexible mechanism 11 is connected to the fixing bracket 15 of the leaf spring, and one end of the second outer stepped leaf spring flexible mechanism 1 is connected to the vibrating terminal 13. When the voice coil motor 12 drives the vibrating terminal 13 to vibrate, the first outer stepped leaf spring flexible mechanism 11 and the second outer stepped leaf spring flexible mechanism 1 play a role in balancing the vibration frequency and amplitude of the transmission mechanism. One end of the middle leaf spring flexible mechanism 9 is connected to the vibrating terminal 13, and the other end is connected to the middle inner leaf spring connecting block 17. The other end of the middle inner leaf spring connecting block 17 is connected to the inner leaf spring flexible mechanism 10, and the other end of the inner leaf spring flexible mechanism 10 is connected to the fixing bracket 15 of the leaf spring. The middle leaf spring flexible mechanism 9, the middle inner leaf spring connecting block 17, the inner leaf spring flexible mechanism 10 work together with the first outer stepped leaf spring flexible mechanism 11 and the second outer stepped leaf spring flexible mechanism 1 to precisely control the vibrating terminal 13 to perform linear reciprocating motion. A pair of square holes 7 on the vibrating terminal 13 contribute to its stability and adaptability during the movement process.

[0039] When the cutting mechanism needs to be used, the vibrating terminal 13 drives the blade clamping mechanism 4 and the blade 6 to perform reciprocating linear motion through the tool holder 2. The tool holder 2 is connected to the vibrating terminal 13 through the threaded hole 8 to ensure the stability of the connection. The blade clamping mechanism 4 is connected to the tool holder 2 through the thread pair 3, and the thread pair 3 of the tool holder 2 is set as a triangular structure, which can enhance the reliability of the connection. When it is necessary to adjust the rotation angle of the blade 6, gaskets can be placed between the vibrating terminal 13 and the tool holder 2 and at the contact surface position between the tool holder 2 and the blade clamping mechanism 4, so as to realize the adjustment of the rotation angle of the blade 6. The blade clamping mechanism 4 includes evenly distributed clamping pieces 5 for firmly clamping the blade 6. If the blade 6 bulges upward or downward, it can be corrected by the corresponding fine adjustment bolt 18. During the cutting process, the sectioning parameters can also be adjusted by adjusting parameters such as the position and angle of the blade 6 to meet different sectioning requirements for large-volume biological samples.

[0040] The preferred embodiments of the present invention described above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principle and practical application of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A vibration sectioning device with a modifiable knife shape for large-volume biological samples, comprising a vibration sectioning device body for cutting a sample (19), characterized in that, It further includes: A driving mechanism, a transmission mechanism, and a cutting mechanism provided on the main body of the vibrating slicing device; The driving mechanism includes a voice coil motor (12), a fixing member (14), and a constraining member (16); The transmission mechanism includes a first outer stepped leaf spring flexible mechanism (11), a second outer stepped leaf spring flexible mechanism (1), a pair of middle leaf spring flexible mechanisms (9), a middle inner leaf spring connecting block (17), a pair of inner leaf spring flexible mechanisms (10), a fixing bracket (15) of the leaf spring, and a vibrating terminal (13); The cutting mechanism includes a tool holder (2), a blade clamping mechanism (4), and a blade (6) connected to the vibrating terminal (13).

2. The vibrating sectioning device with a modifiable knife shape for large-volume biological samples according to claim 1, wherein, The fixing member (14) is connected to the threaded hole on the fixing bracket (15) by a bolt. The diameter of the waist-shaped groove of the fixing member (14) is 1 mm larger than the diameter of the bolt. The voice coil motor (12) realizes reciprocating motion with the assistance of an external cylindrical magnet (1201). The fixing member (14) is used to adjust the distance between the internal coil (1202) and the external cylindrical magnet (1201), and the internal coil (1202) and the constraining member (16) are connected by bolts to drive the vibrating terminal (13) to vibrate together.

3. The vibrating microtome device with a modifiable knife type for large-volume biological samples according to claim 1, characterized in that, One end of the first outer stepped leaf spring flexible mechanism (11) is connected to the fixing bracket (15) of the leaf spring. One end of the second outer stepped leaf spring flexible mechanism (1) is connected to the vibrating terminal (13). One end of the middle leaf spring flexible mechanism (9) is connected to the vibrating terminal (13), and the other end is connected to the middle inner leaf spring connecting block (17). The other end of the middle inner leaf spring connecting block (17) is connected to the inner leaf spring flexible mechanism (10). The other end of the inner leaf spring flexible mechanism (10) is connected to the fixing bracket (15) of the leaf spring. A pair of square holes (7) are provided on the vibrating terminal (13).

4. A vibration sectioning device with a modifiable knife shape for large-volume biological samples according to claim 1, characterized in that, The vibrating terminal (13) drives the blade clamping mechanism (4) and the blade (6) to realize reciprocating linear motion through the tool holder (2). The tool holder (2) is connected to the vibrating terminal (13) through a threaded hole (8). The blade clamping mechanism (4) is connected to the tool holder (2) through a thread pair (3), and the thread pair (3) of the tool holder (2) is set as a triangular structure.

5. The vibrating sectioning device with a modifiable knife shape for large-volume biological samples according to claim 1, characterized in that, A gasket for adjusting the rotation angle of the blade (6) is provided between the vibrating terminal (13) and the tool holder (2). The rotation angle of the blade (6) is adjusted by the gasket at the contact surface position of the tool holder (2) and the blade clamping mechanism (4).

6. The vibrating microtome device with a modifiable knife shape for large-volume biological samples according to claim 1, wherein The blade clamping mechanism (4) includes evenly distributed clamping pieces (5). Fine adjustment bolts (18) that can be tightened or loosened at corresponding positions are provided at the convex or concave positions of the blade (6).

7. A vibration sectioning device with a modifiable knife shape for large-volume biological samples according to claim 1, characterized in that, The voice coil motor (12) on the driving mechanism changes its working state by adjusting the relative position between the internal coil (1202) and the external cylindrical magnet (1201).

8. A vibration slicing device with a modifiable knife shape for large-volume biological samples according to claim 1, characterized in that, The first outer stepped leaf spring flexible mechanism (11) and the second outer stepped leaf spring flexible mechanism (1) of the transmission mechanism are used to balance the vibration frequency and amplitude of the transmission mechanism, and the middle leaf spring flexible mechanism (9), the middle inner leaf spring connecting block (17), the inner leaf spring flexible mechanism (10), the first outer stepped leaf spring flexible mechanism (11), and the second outer stepped leaf spring flexible mechanism (1) are used to control the linear reciprocating motion of the vibrating terminal (13).

9. The vibrating microtome device with a modifiable knife shape for large-volume biological samples according to claim 1, wherein, The cutting mechanism adjusts the slicing state by adjusting the blade (6).

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