Auxiliary device for rapidly marking and positioning biological material sample

Abstract

The invention relates to the technical field of biological samples, and discloses a biological material sample rapid marking and positioning auxiliary device which comprises a base, a control table is fixedly installed at the top of the base, a support is fixedly installed on the front side of the control table, a marking mechanism is arranged on the front side of the control table, and the marking mechanism comprises a first motor. And a first motor is fixedly installed at the top of the support, a lead screw is connected to an output shaft of the first motor, and the lead screw is sleeved with a sliding sleeve. According to the rapid marking and positioning auxiliary device for the biological material sample, through mutual cooperation of all structures of the marking mechanism, a fluorescent agent and the sample are rapidly combined, the effect of rapidly marking the sample is achieved, through arrangement of the positioning system and mutual cooperation of all modules of the positioning system, rapid positioning of a reagent tube is achieved, and the accuracy of marking is improved. Therefore, the test tube with a poor marking effect can be quickly and accurately positioned, and the experiment detection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample materials, and specifically to a rapid labeling and positioning assistance device for biological material samples. Background Art

[0002] Biological sample materials refer to various biological samples and their related data used in medical research, clinical diagnosis, and drug development, including body fluid types, cell and molecular types, and tissue and organ types. Collection principle: It is necessary to ensure representativeness (such as avoiding contaminated areas) and typicality (selecting specific parts according to the research target); storage conditions: Most samples need to be stored frozen at -20°C to -80°C, and some need to add anticoagulants or be centrifuged; Quality control: It is necessary to record clinical information, informed consent documents, and follow standardized operating procedures (SOP).

[0003] Existing biological sample materials are usually labeled with fluorescent reagents. Immunofluorescence labeling technology utilizes the specific recognition and efficient binding between antigens and antibodies, and has a wide range of applications in research fields such as life science and biomedicine. Immunofluorescence technology combines with fluorescence microscopy imaging equipment to achieve visualization and quantitative analysis of biomolecules.

[0004] After retrieval, according to a full-automatic immunofluorescence labeling device and an immunofluorescence labeling method disclosed in the invention patent with Chinese patent number CN118501482A, the full-automatic immunofluorescence labeling device described in the invention patent includes: a control mechanism, a power mechanism, a reagent suction mechanism, and a sample labeling mechanism; the power mechanism is electrically connected to the control mechanism, the reagent suction mechanism and the sample labeling mechanism are both connected to the power mechanism, and the power mechanism is used to drive the reagent suction mechanism to suck the reagents required for immunofluorescence labeling and transport the reagents required for immunofluorescence labeling to the sample labeling mechanism; the sample labeling mechanism is used to add the reagents required for immunofluorescence labeling to the corresponding culture dish to be labeled, realizing the steps of automatically performing immunofluorescence labeling.

[0005] However, during the process of adding fluorescent agents, this device requires a large number of pipelines to be connected to different probes, and the device wiring is disordered. Once a certain pipeline is damaged and cannot be discovered in time, it will lead to the failure of adding fluorescent agents, and it is impossible to quickly and accurately position the samples to be detected, thus reducing the use efficiency of the device. At the same time, this device cannot quickly fuse the samples and reagents, resulting in low sample labeling efficiency. Therefore, a rapid labeling and positioning assistance device for biological material samples is proposed to solve the above-mentioned problems. Summary of the Invention

[0006] (1) Technical Problems to be Solved

[0007] In view of the deficiencies of the prior art, the present invention provides a rapid marking and positioning assistance device for biological material samples, which has the advantages of facilitating the rapid marking and positioning of samples, and solves the problems that a large number of pipelines need to be connected to different probes during the addition of fluorescent agents, the device wiring is disordered, and once a certain pipeline is damaged, it cannot be detected in time, which will lead to the failure of fluorescent agent addition, and the samples to be detected cannot be accurately positioned quickly, thus reducing the use efficiency of the device. At the same time, the device cannot quickly fuse the samples and reagents, resulting in low sample marking efficiency.

[0008] Technical solution

[0009] To achieve the purpose of rapid marking and positioning of the above samples, the present invention provides the following technical solution: A rapid marking and positioning assistance device for biological material samples, including a base, a control console is fixedly installed on the top of the base, a bracket is fixedly installed on the front side of the control console, and a marking mechanism is arranged on the front side of the control console;

[0010] The marking mechanism includes a first motor, a lead screw, a sliding sleeve, a placement table, a reagent box, a metering cylinder, a probe body, a second motor, a turntable, a test tube, a first support, a first electrode plate, a second support, a second electrode plate and a pressing plate. A first motor is fixedly installed on the top of the bracket, a lead screw is connected to the output shaft of the first motor, a sliding sleeve is sleeved outside the lead screw, a placement table is connected to the outside of the sliding sleeve, a reagent box is installed on the top of the placement table, a metering cylinder is fixedly installed on the top of the placement table, a probe body is installed inside the placement table body, a second motor is fixedly installed on the top of the base, a turntable is connected to the output shaft of the second motor, a test tube is movably installed inside the turntable, a first support is installed inside the test tube, a first electrode plate is installed inside the first support, a second support is installed on the top of the first support, a second electrode plate is installed inside the second support, and a pressing plate is installed on the top of the second support.

[0011] Preferably, a positioning system is arranged on the top of the base, and the positioning system includes a main control module, an RFID tag module, a data acquisition module, a data processing module, a tag positioning model module and a result output module;

[0012] Among them, a main control module is arranged inside the control console, and the main control module controls the RFID tag module, the data acquisition module, the data processing module, the tag positioning model module and the result output module, so that each module works in cooperation to achieve accurate positioning of the test tube.

[0013] Preferably, the RFID tag module precisely locates each test tube by implanting an RFID tag in the test tube. The data acquisition module uses an RFID reader to collect data from the RFID tag, import the data into the data processing module, and the tag positioning model module determines the positions of the tags and imports the results into the console.

[0014] Preferably, one side of the lead screw away from the first motor is rotatably connected to the bottom of the base, and a chute is provided inside the bracket.

[0015] Preferably, a limit block is installed on the left side of the sliding sleeve, and the side of the limit block away from the sliding sleeve is slidably connected to the chute. A first conduit connecting the right side of the reagent box to the left side of the metering cylinder is provided.

[0016] Preferably, a second conduit connecting the right side of the metering cylinder to the left side of the probe body is provided. Solenoid valves are installed on the outer parts of both the first conduit and the second conduit, and delivery pumps are installed on the outer parts of both the first conduit and the second conduit.

[0017] Preferably, the probe body includes a first needle rod and a second needle rod, and the first needle rod and the second needle rod are threadedly connected.

[0018] Preferably, a placement groove is provided inside the turntable, the test tube is located inside the placement groove, a card hole is provided at the top of the first support, and a clamping rod is connected to the bottom of the second support.

[0019] Preferably, the first electrode plate is threadedly connected to the first support, the second electrode plate is threadedly connected to the second support, a sample body is provided inside the test tube, the sample body is located between the first electrode plate and the second electrode plate, an electric push rod is fixedly installed inside the metering cylinder, and a pressing plate is fixedly installed at the bottom of the electric push rod.

[0020] Advantages

[0021] Compared with the prior art, the present invention provides a rapid marking and positioning assistance device for biological material samples, having the following advantages:

[0022] 1. In the rapid marking and positioning assistance device for biological material samples, through the cooperation of the various structures of the marking mechanism, the fluorescent agent and the sample are quickly combined, thus achieving the effect of quickly marking the sample.

[0023] 2. In the rapid marking and positioning assistance device for biological material samples, by setting up a positioning system and the cooperation of the various modules of the positioning system, the reagent tubes can be quickly positioned, and thus the test tubes with poor marking effects can be accurately positioned quickly, improving the experimental detection efficiency. Brief Description of the Drawings

[0024] Figure 1 This is the three-dimensional front view schematic diagram of the present invention;

[0025] Figure 2 This is the schematic diagram of the marking mechanism of the present invention;

[0026] Figure 3 This is the sectional view schematic diagram of the present invention;

[0027] Figure 4 This is the schematic diagram of the local marking mechanism of the present invention;

[0028] Figure 5 This is the three-dimensional schematic diagram of the first support of the present invention;

[0029] Figure 6 This is the three-dimensional schematic diagram of the second support of the present invention;

[0030] Figure 7 This is the schematic diagram of the positioning system of the present invention.

[0031] In the figure: 1 base, 2 control console, 3 bracket, 4 marking mechanism, 401 first motor, 402 lead screw, 403 sliding sleeve, 404 placement table, 405 reagent box, 406 measuring cylinder, 407 probe body, 4071 first needle rod, 4072 second needle rod, 408 second motor, 409 turntable, 410 test tube, 411 first support, 412 first electrode plate, 413 second support, 414 second electrode plate, 415 pressing plate, 5 first conduit, 6 second conduit, 7 clamping hole, 8 clamping rod, 9 electric push rod, 10 pressing plate. Detailed Description of the Invention

[0032] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0033] Please refer to Figure 1-7 , a rapid marking and positioning assistance device for biological material samples, including a base 1, a control console 2 fixedly installed on the top of the base 1, a bracket 3 fixedly installed on the front side of the control console 2, and a marking mechanism 4 arranged on the front side of the control console 2;

[0034] The labeling mechanism 4 includes a first motor 401, a lead screw 402, a sliding sleeve 403, a placement table 404, a reagent box 405, a measuring cylinder 406, a probe body 407, a second motor 408, a turntable 409, a test tube 410, a first support 411, a first electrode plate 412, a second support 413, a second electrode plate 414, and a pressing plate 415. A first motor 401 is fixedly installed at the top of the bracket 3. A lead screw 402 is connected to the output shaft of the first motor 401. A sliding sleeve 403 is sleeved outside the lead screw 402. A placement table 404 is connected to the outside of the sliding sleeve 403. A reagent box 405 is installed on the top of the placement table 404. A measuring cylinder 406 is fixedly installed on the top of the placement table 404. A probe body 407 is installed inside the placement table body 1. A second motor 408 is fixedly installed at the top of the base 1. A turntable 409 is connected to the output shaft of the second motor 408. A test tube 410 is movably installed inside the turntable 409. A first support 411 is installed inside the test tube 410. A first electrode plate 412 is installed inside the first support 411. A second support 413 is installed on the top of the first support 411. A second electrode plate 414 is installed inside the second support 413. A pressing plate 415 is installed on the top of the second support 413.

[0035] In Figure 7 it, a positioning system is provided at the top of the base 1. The positioning system includes a main control module, an RFID tag module, a data acquisition module, a data processing module, a tag positioning model module, and a result output module;

[0036] Among them, a main control module is provided inside the console 2. The RFID tag module, the data acquisition module, the data processing module, the tag positioning model module, and the result output module are controlled through the main control module, so that each module works in coordination to achieve precise positioning of the test tube 410. The RFID tag module realizes precise positioning of each test tube 410 by implanting an RFID tag in the test tube 410. The data acquisition module, the RFID reader can collect data from the RFID tag, import the data into the data processing module, and the tag positioning model module judges the positions of each tag and imports the results into the console 2.

[0037] Specifically, by setting up the positioning system, the modules of the positioning system cooperate with each other to quickly locate the reagent tube 410, and then accurately locate the test tubes 410 with poor labeling effects, improving the experimental detection efficiency.

[0038] I. Data acquisition module

[0039] Label signal acquisition

[0040] Deploy RFID readers and antenna arrays to receive parameters such as RSSI / PDOA of the radio frequency signals reflected by the tags in real time;

[0041] Support active tags to actively emit signals or passive tags to passively reflect signals 58;

[0042] Construction of environmental fingerprint database

[0043] Establish a two-dimensional / three-dimensional radio frequency signal fingerprint database in the positioning area and record the signal characteristics at different positions.

[0044] II. Data processing module

[0045] Data preprocessing

[0046] Filter and normalize the collected RSSI / PDOA signals to eliminate noises such as multipath interference 3;

[0047] Tag positioning model module

[0048] Use convolutional neural networks such as ResNet, U-Net, etc. to extract the spatial features of signals 3;

[0049] The input layer receives joint fingerprint data such as RSSI+PDOA matrix, and the output layer predicts the tag coordinates 3.

[0050] III. Online positioning module

[0051] Real-time signal analysis

[0052] Obtain the real-time signal of the tag to be positioned through the reader and convert it into the model input format;

[0053] Position calculation and optimization

[0054] The CNN model infers the tag position and combines algorithms such as Kalman filtering to optimize the positioning accuracy.

[0055] IV. System management module

[0056] Visualization interface

[0057] Display the real-time position, movement trajectory of the tag and warning information such as out-of-bounds prompt;

[0058] Network communication

[0059] Transmit the positioning data to the backend server through Ethernet / WLAN;

[0060] Such systems are especially suitable for complex indoor environments such as subway stations. Compared with the traditional triangulation method, CNN can effectively cope with the multipath effect, and the positioning error can be reduced by 30%-50%.

[0061] In Figure 1 and Figure 2Among them, one side of the lead screw 402 away from the first motor 401 is rotatably connected to the bottom of the base 1, and a chute is provided inside the bracket 3.

[0062] Specifically, when the controller drives the first motor 401 to start, it can drive the lead screw 402 to rotate, so that the sliding sleeve 403 drives the placement table 404 to lift, enabling the probe body 407 to pierce into the test tube 410 to achieve the delivery of the fluorescent agent.

[0063] In Figure 1 and Figure 3 Among them, a limit block is installed on the left side of the sliding sleeve 403, and one side of the limit block away from the sliding sleeve 403 is slidably connected to the chute. The right side of the reagent box 405 is connected to a first conduit 5 that is interconnected with the left side of the metering cylinder 406.

[0064] Specifically, by setting the metering cylinder 406, when the controller drives the electric push rod 9 to start, the telescopic movement of the electric push rod 9 can drive the pressing plate 10 to move downward, so that the reagent enters the probe body 407 through the second conduit 6, which can ensure that the same amount of reagent is added to the test tubes of the same batch and avoid poor labeling effects caused by uneven feeding.

[0065] In Figure 3 and Figure 4 Among them, the right side of the metering cylinder 406 is connected to a second conduit 6 that is interconnected with the left side of the probe body 407. Solenoid valves are installed on the outside of both the first conduit 5 and the second conduit 6, and delivery pumps are installed on the outside of both the first conduit 5 and the second conduit 6.

[0066] Specifically, by setting the second motor 408, when the controller drives the second motor 408 to start, it drives the turntable 409 to rotate, moving the reagent tube 410 to directly below the probe body 407, thus facilitating the implantation of the reagent.

[0067] In Figure 2 and Figure 4 Among them, the probe body 407 includes a first needle rod 4071 and a second needle rod 4072, and the first needle rod 4071 and the second needle rod 4072 are threadedly connected.

[0068] In Figure 4 and Figure 5 Among them, a placement groove is provided inside the turntable 409, the test tube 410 is located inside the placement groove, a card hole 7 is provided at the top of the first support 411, and a card rod 8 is connected to the bottom of the second support 413.

[0069] In Figure 5 and Figure 6In it, the first electrode sheet 412 is threadedly connected to the first support 411, the second electrode sheet 414 is threadedly connected to the second support 413, a sample body is arranged inside the test tube 410, and the sample body is located between the first electrode sheet 412 and the second electrode sheet 414. An electric push rod 9 is fixedly installed inside the metering cylinder 406, and a pressing plate 10 is fixedly installed at the bottom of the electric push rod 9

[0070] In summary, when using this rapid marking and positioning assistance device for biological material samples, first connect the first support 411 with the first electrode sheet 412 and then tighten the second support 413 and the second electrode sheet 414. At this time, place the sample in the first support 411, move the second support 413 downward so that the clamping rod 8 enters the clamping hole 7, and install a pressing plate 415 on the top of the second support 413. At this time, close the test tube 410 and place it in the turntable 409, and fill the turntable 409 in sequence. When marking the sample, the controller drives the first motor 401 to start, and drives the probe body 407 to move downward through the sliding sleeve 403 and insert it into the test tube 410. At this time, the solenoid valve starts, the reagent enters the metering cylinder 406 from the reagent tank 405, and is extruded by the pressing plate 10 and enters the test tube 410 through the probe body 407. At this time, the first electrode sheet 412 and the second electrode sheet 414 are electrified, so that the sample and the reagent are quickly fused to achieve the effect of rapid marking.

[0071] Moreover, by setting the first motor 401, the controller drives the first motor 401 to start, which can drive the lead screw 402 to rotate, so that the sliding sleeve 403 drives the placement table 404 to lift and lower, enabling the probe body 407 to pierce into the test tube 410 to realize the delivery of the fluorescent agent. By setting the metering cylinder 406, the controller drives the electric push rod 9 to start, and the telescopic movement of the electric push rod 9 can drive the pressing plate 10 to move downward, so that the reagent enters the probe body 407 from the second conduit 6, which can ensure that the same amount of reagent is added to the test tubes of the same batch, avoiding poor marking effects caused by uneven feeding. By setting the second motor 408, the controller drives the second motor 408 to start and drive the turntable 409 to rotate, moving the reagent tube 410 directly below the probe body 407, thus facilitating the implantation of the reagent. By setting the first support 411 and the second support 413 to limit the first electrode sheet 412 and the second electrode sheet 414, when the reagent is injected into the test tube 410 and flows between the first electrode sheet 412 and the second electrode sheet 414 through the pressing plate 415, at this time, the first electrode sheet 412 and the second electrode sheet 414 are electrified, so that the sample and the reagent are quickly fused to achieve the effect of rapid marking.

[0072] It should be noted that, in this document, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the element.

[0073] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A rapid labeling and positioning assistance device for biological material samples, comprising a base (1), characterized in that: A console (2) is fixedly installed at the top of the base (1), a bracket (3) is fixedly installed on the front side of the console (2), and a marking mechanism (4) is arranged on the front side of the console (2). The marking mechanism (4) includes a first motor (401), a lead screw (402), a sliding sleeve (403), a placement table (404), a reagent box (405), a measuring cylinder (406), a probe body (407), a second motor (408), a turntable (409), a test tube (410), a first support (411), a first electrode plate (412), a second support (413), a second electrode plate (414) and a pressing plate (415). A first motor (401) is fixedly installed at the top of the bracket (3), a lead screw (402) is connected to the output shaft of the first motor (401), a sliding sleeve (403) is sleeved outside the lead screw (402), a placement table (404) is connected to the outside of the sliding sleeve (403), a reagent box (405) is installed on the top of the placement table (404), a measuring cylinder (406) is fixedly installed on the top of the placement table (404), a probe body (407) is installed inside the placement table body (1), a second motor (408) is fixedly installed at the top of the base (1), a turntable (409) is connected to the output shaft of the second motor (408), a test tube (410) is movably installed inside the turntable (409), a first support (411) is installed inside the test tube (410), a first electrode plate (412) is installed inside the first support (411), a second support (413) is installed on the top of the first support (411), a second electrode plate (414) is installed inside the second support (413), and a pressing plate (415) is installed on the top of the second support (413).

2. The rapid labeling and positioning assistance device for a biological material sample according to claim 1, wherein: A positioning system is arranged at the top of the base (1), and the positioning system includes a main control module, an RFID tag module, a data acquisition module, a data processing module, a tag positioning model module and a result output module; Among them, a main control module is arranged inside the console (2), and the RFID tag module, the data acquisition module, the data processing module, the tag positioning model module and the result output module are controlled by the main control module, so that each module works in cooperation to achieve precise positioning of the test tube (410).

3. The rapid marking and positioning assistance device for a biological material sample according to claim 2, wherein: The RFID tag module realizes precise positioning of each test tube (410) by implanting an RFID tag in the test tube (410). The data acquisition module can collect data of the RFID tag by an RFID reader, import the data into the data processing module, and the tag positioning model module judges the positions of the tags and imports the results into the console (2).

4. A rapid labeling and positioning assistance device for biological material samples according to claim 1, characterized in that: One side of the lead screw (402) away from the first motor (401) is rotatably connected to the bottom of the base (1), and a chute is opened inside the bracket (3).

5. The rapid labeling and positioning assistance device for a biological material sample according to claim 4, wherein: A limiting block is installed on the left side of the sliding sleeve (403). The side of the limiting block away from the sliding sleeve (403) is slidably connected to the chute. The right side of the reagent box (405) is connected to a first conduit (5) that is interconnected with the left side of the metering cylinder (406).

6. The rapid labeling and positioning assistance device for a biological material sample according to claim 1, characterized in that: The right side of the metering cylinder (406) is connected to a second conduit (6) that is interconnected with the left side of the probe body (407). Solenoid valves are installed on the exteriors of both the first conduit (5) and the second conduit (6). Delivery pumps are installed on the exteriors of both the first conduit (5) and the second conduit (6).

7. A rapid labeling and positioning assistance device for biological material samples according to claim 1, characterized in that: The probe body (407) includes a first needle rod (4071) and a second needle rod (4072). The first needle rod (4071) and the second needle rod (4072) are threadedly connected.

8. A rapid marking and positioning assistance device for a biological material sample according to claim 1, characterized in that: A placement groove is formed inside the turntable (409). The test tube (410) is located inside the placement groove. A clamping hole (7) is formed at the top of the first support (411). A clamping rod (8) is connected to the bottom of the second support (413).

9. A rapid labeling and positioning assistance device for biological material samples according to claim 1, characterized in that: The first electrode plate (412) is threadedly connected to the first support (411). The second electrode plate (414) is threadedly connected to the second support (413). A sample body is disposed inside the test tube (410). The sample body is located between the first electrode plate (412) and the second electrode plate (414). An electric push rod (9) is fixedly installed inside the metering cylinder (406). A pressing plate (10) is fixedly installed at the bottom of the electric push rod (9).

Images