Platelet aggregation function detection kit
By using a biochemically active membrane composed of collagen and raptorycin, combined with capillaries and sample pools, the problem in the prior art that the blood outflow to the clotting process cannot be accurately simulated, and the precise measurement of platelet aggregation function is achieved.
Patent Information
- Application Number
- CN202510616932.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-14
- Publication Date
- 2025-08-01
AI Technical Summary
The prior art is difficult to accurately simulate the process of blood flowing out of blood vessels to coagulation in vitro, and it is impossible to effectively measure the time point of platelet aggregation function.
Using a biochemically active membrane containing collagen and raptorycin, combined with capillaries and sample pools, simulates the blood flow out of the blood vessels to the coagulation process, and measures the time when platelet emboli blocks pores as a quantitative indicator of platelet aggregation function.
The accurate measurement of platelet aggregation function in vitro is achieved, providing more accurate platelet function analysis.
Smart Images
Figure CN120405154A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of computer technology, and more particularly, to a kit for detecting platelet aggregation function. Background Art
[0002] At present, since the formation of platelet emboli can gradually reduce blood flow and ultimately block it, platelets are an important product of the human body. It is necessary to determine how to accurately simulate the process of blood flowing out of blood vessels and coagulating in vitro. Another problem is how to determine the time from the start of the test to the occlusion of the pores by platelet emboli and measure the platelet aggregation function. Summary of the Invention
[0003] The purpose of the present invention is to provide a kit for detecting platelet aggregation function to solve the above problems existing in the prior art.
[0004] An embodiment of the present invention provides a kit for detecting platelet aggregation function, characterized by comprising a sample pool and a biochemical activity membrane assembly: The sample pool is a cavity capable of accommodating citrate-anticoagulated whole blood; the top of the sample pool is a sample addition port; the sample addition port is separated from the biochemical activity membrane assembly at the top; The biochemical activity membrane assembly has a biochemical activity membrane laid flat in the center; there is an opening at the position where the biochemical activity membrane is laid flat in the biochemical activity membrane assembly; the biochemical activity membrane assembly communicates the biochemical activity membrane with the sample pool through the opening; There is a membrane pore in the center of the biochemical activity membrane; the membrane pore is a round hole; the membrane pore can suck citrate-anticoagulated whole blood from the sample pool; The biochemical activity membrane includes a membrane surface coating; the membrane surface coating is composed of a combination of collagen and ristocetin; the collagen is a subendothelial protein; the ristocetin and collagen are used for platelet activation in the aggregation test.
[0005] Optionally, it further includes a capillary tube; The capillary tube is fixed below the biochemical activity membrane assembly; the capillary tube is placed above the citrate-anticoagulated whole blood in the sample pool; The capillary tube is used to suck the citrate-anticoagulated whole blood in the sample pool and pass through the biochemical activity membrane through the membrane pore; Among them, the platelets of the citrate-anticoagulated whole blood sucked from the sample pool through the capillary tube and the membrane pore are in a high shear flow state.
[0006] Optionally, 25 μl of a mixed solution is dripped in the middle of the round hole of the biochemical activity membrane; The mixed solution is a solution obtained by mixing a collagen solution and a ristocetin solution in a ratio of 1:1; The mixed solution is evenly spread on the biochemical active membrane.
[0007] Optionally, the ristocetin can initiate and release particulate matter; the particulate matter can cause platelets to adhere to each other to form aggregates; The aggregates form platelet plugs in the membrane pores; the platelet plugs gradually reduce the blood flow until the blood flow is blocked; the aggregates can be used to measure platelet aggregation function.
[0008] Optionally, obtain the closing time; the closing time represents the time from the start of the aggregation test to the time when the platelet plug blocks the membrane pores and blocks the blood flow; The closing time can be used as a quantitative index of platelet aggregation function.
[0009] Optionally, the mixed solution in the biochemical active membrane has been uniformly dried.
[0010] Optionally, the content range of collagen after drying of 25 μl of the mixed solution is 0.1 to 10 μg, and the content range of ristocetin is 0.1 to 50 μg.
[0011] Optionally, the sample pool, the biochemical active membrane assembly and the capillary are encapsulated into a sealed system; the sealed system is sealed at the upper opening by an upper opening sealer and at the bottom opening by a bottom opening sealer; The sealed system and the desiccant are placed in an aluminum foil bag; The aluminum foil bag containing the sealed system and the desiccant is a sealed aluminum foil bag; The aluminum foil bag is stored at a temperature of 2-8 °C.
[0012] Optionally, the sample inlet is communicated with the sample pool; The sample inlet can be used to add citrate anticoagulated whole blood into the sample pool.
[0013] Optionally, an analyzer: The biochemical active membrane assembly that can place the citrate anticoagulated whole blood inhaled into the sample pool can be placed in the analyzer; The analyzer includes a vacuum pump assembly; The vacuum pump assembly can automatically adjust the pressure parameters to keep the sealed system at a constant negative pressure value; the constant negative pressure value is preset; The vacuum pump assembly can judge whether a thrombus is completely formed to block the hole and obtain the blood flow velocity according to the pressure parameters and the constant negative pressure value.
[0014] Compared with the prior art, the embodiments of the present invention achieve the following beneficial effects: The embodiments of the present invention also provide a platelet aggregation function detection kit.
[0015] According to the present invention, a biochemical active membrane composed of two platelet inducers, collagen and ristocetin, can more accurately determine the platelet aggregation state. The special structure composed of a capillary, a biochemical active membrane assembly, a sample pool and a biochemical active membrane can simulate the process of blood flowing out of blood vessels to coagulation in vitro. The time from the start of the aggregation test to the occlusion of the pores by platelet plugs is detected, and this time duration is used as the closing time. The closing time is used as a quantitative index of platelet aggregation function. The technical effect of more accurately measuring the platelet function in the platelet function analysis system is achieved. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 FIG. 1 is a schematic front view of the structure of a platelet aggregation function detection kit provided by an embodiment of the present invention when it is opened.
[0017] Figure 2 FIG. 2 is a schematic structural view of a capillary in a platelet aggregation function detection kit provided by an embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION
[0018] The present invention will be described in detail below with reference to the accompanying drawings.
[0019] Example 1 As Figure 1 shown, an embodiment of the present invention provides a platelet aggregation function detection kit, including a sample pool and a biochemical active membrane assembly: The sample pool is a cavity capable of accommodating citrate anticoagulated whole blood; the top of the sample pool is a sampling port; the sampling port is separated from the biochemical active membrane assembly at the top; A biochemical active membrane is laid flat in the center of the biochemical active membrane assembly; there is an opening at the position where the biochemical active membrane is laid flat in the biochemical active membrane assembly; the biochemical active membrane assembly communicates the biochemical active membrane with the sample pool through the opening; There is a membrane pore in the center of the biochemical active membrane; the membrane pore is a round hole; the membrane pore can suck citrate anticoagulated whole blood from the sample pool.
[0020] Among them, in this embodiment, the area of the round hole is 1 / 1000 of the area of the biochemical active membrane.
[0021] The biochemical active membrane includes a membrane surface coating; the membrane surface coating is composed of a combination of collagen and ristocetin; the collagen is a subendothelial protein; the ristocetin and collagen are used for platelet activation in the aggregation test.
[0022] Among them, the collagen is the initial matrix for platelet adhesion, and the adhesion of platelets to collagen is the initial physiological stimulus that triggers platelet activation.
[0023] Optionally, a capillary is further included.
[0024] Among them, the structure of the capillary is as Figure 2 shown.
[0025] The capillary is fixed below the biochemical active membrane assembly; the capillary is placed above the citrate anticoagulated whole blood in the sample pool.
[0026] The capillary is used to inhale the citrate anticoagulated whole blood in the sample pool and pass through the membrane pores through the biochemical active membrane.
[0027] Among them, the platelets of the citrate anticoagulated whole blood inhaled from the sample pool through the capillary and the membrane pores are in a high-shear flow state.
[0028] Optionally, 25 μl of the mixed solution is dripped in the middle of the round hole of the biochemical active membrane.
[0029] Among them, when the mixed solution is dripped, a pipette is used to aspirate it.
[0030] The mixed solution is a solution obtained by mixing a collagen solution and a ristocetin solution in a ratio of 1:1; The mixed solution is evenly spread inside the biochemical active membrane.
[0031] Among them, the mixed solution shall not flow out of the boundary between the inside and the outside of the biochemical active membrane.
[0032] Optionally, the ristocetin can initiate and release particulate matter; the particulate matter can cause platelets to adhere to each other to form aggregates; The aggregates form platelet plugs in the membrane pores; the platelet plugs gradually reduce the blood flow until the blood flow is blocked; The aggregates can be used to measure the platelet aggregation function.
[0033] Optionally, the closure time is obtained; the closure time represents the time from the start of the aggregation test to the time when the platelet plug blocks the membrane pores and blocks the blood flow; The closure time can be used as a quantitative index for the platelet aggregation function.
[0034] Among them, the platelet aggregation function means that platelets adhere to each other to form aggregates, gradually reducing the blood flow until the blood flow is blocked.
[0035] Optionally, the mixed solution in the biochemical active membrane has been uniformly dried.
[0036] Among them, in this embodiment, after all the biochemical active membranes are dripped with the mixed solution, a vacuum drying oven is used for uniform drying. A desiccant is placed in the vacuum drying oven, and the biochemical active membrane assembly dripped with the mixed solution is placed in the vacuum drying oven and dried for more than 10 hours.
[0037] Among them, the biochemical activity membrane is coated with a mixed solution and dried.
[0038] Optionally, the content of collagen after drying 25 μl of the mixed solution ranges from 0.1 to 10 μg, and the content of ristocetin ranges from 0.1 to 50 μg.
[0039] Optionally, the structure composed of the analyzer, sample cell, biochemical activity membrane assembly, and capillary after sealing and the desiccant are placed in an aluminum foil bag; The aluminum foil bag containing the analyzer, sample cell, biochemical activity membrane assembly, capillary structure, and desiccant is a sealed aluminum foil bag; The aluminum foil bag is stored at a temperature of 2 - 8 °C.
[0040] Optionally, it further includes a sample addition port; The sample addition port communicates with the sample cell; The sample addition port can be used to add citrate anticoagulated whole blood into the sample cell.
[0041] Optionally, it further includes an analyzer: The sample cell, biochemical activity membrane assembly, and capillary are encapsulated into a sealed system; the sealed system seals the upper opening through an upper opening sealer and seals the bottom opening through a bottom opening sealer.
[0042] The analyzer includes a vacuum pump assembly; The vacuum pump assembly can automatically adjust the pressure parameters to keep the sealed system at a constant negative pressure value; the constant negative pressure value is preset; The vacuum pump assembly can judge whether the thrombus completely forms to seal the hole and obtain the blood flow velocity according to the pressure parameters and the constant negative pressure value.
[0043] Among them, when the citrate anticoagulated whole blood is drawn out from the sample cell through the capillary and the round hole of the biochemical activity membrane, the platelets are exposed to a high - speed shear flow state. When the platelets gradually form platelet plugs in the membrane pores, the blood flow will continuously decrease. To maintain the constant negative pressure value, the sealed system will automatically adjust the pressure parameters.
[0044] Example 2 The detection principle is that the platelet aggregation function detection kit (collagen / ristocetin - triggered closure time method) simulates the process of blood flowing out of blood vessels to coagulation in vitro, and is composed of a series of integrated components, including a sample cell, a capillary, and a biochemical activity membrane assembly with a round hole in the center.
[0045] Citrate anticoagulated whole blood is aspirated from the sample pool through a capillary and the pores of the membrane. During this process, platelets are exposed to a high shear flow state. The collagen coated on the membrane surface is a subendothelial protein and is widely regarded as the initial matrix for platelet attachment. The attachment of platelets to collagen is the initial physiological stimulus that triggers platelet activation. In addition, the membrane surface is also coated with ristocetin, and ristocetin and collagen are widely used together for platelet activation in aggregation tests. It starts and releases its granular substances. With the release of the granular substances, platelets adhere to each other to form aggregates. The aggregation of platelets leads to the formation of platelet plugs at the pores, thereby gradually reducing the blood flow and ultimately blocking it, which is used as a method to measure platelet function in a platelet function analysis system.
[0046] The platelet aggregation function detection kit (collagen / ristocetin-triggered closure time method) measures the time from the start of the test to the occlusion of the pores by platelet plugs, and reports this duration as the closure time (CT). The closure time is used as a quantitative indicator of platelet aggregation function.
[0047] The kit consists of a sample pool, a capillary, and a biochemical activity membrane assembly with a round hole in the center. The biochemical activity membrane is coated with a liquid medicine and dried.
[0048] The detection device used is the HaFe 210 platelet analyzer.
[0049] The analysis principle of the testing equipment is that the analyzer and the test sample pool form a sealed system. Through the vacuum pump component of the analyzer, the system maintains a constant negative pressure value set as expected. The sample is drawn out from the storage area of the test pool through the capillary and the round hole of the biochemical activity membrane, and the platelets are exposed to a high-speed shear flow state. When platelets gradually form platelet plugs in the holes, the blood flow will continuously decrease. To maintain the constant negative pressure value, the sealed system will automatically adjust the pressure parameters. The analyzer calculates the blood flow velocity through the pressure compensation parameters and the pressure value, and determines whether a thrombus is completely formed to block the hole. The instrument measures the time from the start to the formation of a platelet plug to seal the hole, that is, the closure time (Closure Time; CT), which is used as an indicator of platelet function.
[0050] The production equipment includes a bottom sealer, a top sealer, and a vacuum drying oven. Kit production First, prepare the liquid medicine. The range of the collagen solution is 0.1 - 10 μg, and the range of the ristocetin solution is 0.1 - 50 μg.
[0051] The attachment of platelets to collagen is the initial physiological stimulus that triggers platelet activation. In addition, the membrane surface is also coated with ristocetin, and ristocetin and collagen are widely used together for platelet activation in aggregation tests. Start and release its granular substances. With the release of the granular substances, platelets adhere to each other to form aggregates.
[0052] Second, perform dotting on the membrane and drying. Mix the prepared collagen solution and ristocetin solution in a 1:1 ratio. Use a pipette to aspirate 25 μl of the mixed solution and sequentially dot it in the middle of each biochemical activity membrane with a round hole in the center. The liquid droplet should be evenly spread around the round membrane and not flow out of the edge. After all the biochemical activity membranes are dotted, perform drying uniformly. Place a desiccant in the vacuum drying oven, and put the integrated biochemical activity membrane with the applied liquid into the vacuum drying oven for drying for more than 10 hours.
[0053] Third, perform assembly and sealing. Seal the bottom opening of the sample cell with a bottom sealing machine. Assemble the sample cell with a capillary and an integrated biochemical activity membrane with a round hole in the center. Seal the upper opening of the sample cell with an upper sealing machine.
[0054] Third, perform storage. Put the sealed sample cell into an aluminum foil bag, place a desiccant in the aluminum foil bag, seal the aluminum foil bag, and store it in a refrigerator at 2-8 °C.
Claims
1. A platelet aggregation function detection kit, characterized in that, It includes a sample pool and a biochemical activity membrane assembly: The sample pool is a cavity capable of accommodating citrate anticoagulated whole blood; the top of the sample pool is a sample addition port; the sample addition port is partitioned from the biochemical activity membrane assembly at the top; A biochemical activity membrane is spread flat in the center of the biochemical activity membrane assembly; There is an opening at the position where the biochemical activity membrane is spread flat in the biochemical activity membrane assembly; The biochemical activity membrane assembly communicates the biochemical activity membrane with the sample pool through the opening; There is a membrane pore in the center of the biochemical activity membrane; the membrane pore is a round hole; the membrane pore can suck citrate anticoagulated whole blood from the sample pool; The biochemical activity membrane includes a membrane surface coating; the membrane surface coating is composed of a combination of collagen and ristocetin; the collagen is a subendothelial protein; the ristocetin and collagen are used for platelet activation in the aggregation test.
2. The platelet aggregation function detection kit according to claim 1, characterized in that, It also includes a capillary; The capillary is fixed below the biochemical activity membrane assembly; the capillary is placed above the citrate anticoagulated whole blood in the sample pool; The capillary is used to suck the citrate anticoagulated whole blood in the sample pool and pass through the biochemical activity membrane through the membrane pore; Among them, the platelets of the citrate anticoagulated whole blood sucked from the sample pool through the capillary and the membrane pore are in a high shear flow state.
3. The platelet aggregation function detection kit according to claim 1, characterized in that, 25 μl of the mixed solution is dripped in the middle of the round hole of the biochemical activity membrane; The mixed solution is a solution obtained by mixing a collagen solution and a ristocetin solution in a ratio of 1:1; The mixed solution is evenly spread on the biochemical activity membrane.
4. The platelet aggregation function detection kit according to claim 2, characterized in that, The ristocetin can initiate and release particulate matter; the particulate matter can cause platelets to adhere to each other to form aggregates; The aggregates form platelet plugs in the membrane pores; the platelet plugs gradually reduce the blood flow until the blood flow is blocked; the aggregates can be used to measure the platelet aggregation function.
5. The platelet aggregation function detection kit according to claim 4, wherein, Obtain the closure time; the closure time represents the time from the start of the aggregation test to the platelet plug blocking the membrane pore and blocking the blood flow; The closure time can be used as a quantitative index for platelet aggregation function.
6. The platelet aggregation function detection kit according to claim 1, characterized in that The mixed solution in the biochemical activity membrane has been uniformly dried.
7. The method of the platelet aggregation function detection kit according to claim 6, wherein, The content range of collagen after drying of 25 μl of the mixed solution is 0.1 to 10 μg, and the content range of ristocetin is 0.1 to 50 μg.
8. The method of the platelet aggregation function detection kit according to claim 3, wherein The sample pool, the biochemical activity membrane assembly and the capillary are encapsulated into a sealed system; the sealed system is sealed at the upper opening by an upper opening sealer and at the bottom opening by a bottom opening sealer; The sealed system and the desiccant are placed in an aluminum foil bag; The aluminum foil bag containing the sealed system and the desiccant is a sealed aluminum foil bag; The aluminum foil bag is stored at a temperature of 2 - 8 °C.
9. The method of the platelet aggregation function detection kit according to claim 1, wherein The sample addition port communicates with the sample pool; The sample addition port can be used to add citrate anticoagulated whole blood to the sample pool.
10. A platelet aggregation function detection kit according to claim 8, characterized in that, Analyzer: The biochemical activity membrane assembly that can suck the citrate anticoagulated whole blood in the sample pool can be placed in the analyzer; The analyzer includes a vacuum pump assembly; The vacuum pump assembly can automatically adjust the pressure parameters to keep the sealed system at a constant negative pressure value; the constant negative pressure value is preset; The vacuum pump assembly can judge whether a thrombus is completely formed to block the hole and obtain the blood flow rate according to the pressure parameters and the constant negative pressure value.