Ultrasonic-assisted method for separating shell meat of euphausia superba

Abstract

The application discloses a kind of based on ultrasonic auxiliary Euphausia Superba shell meat separation method.The shell meat separation method includes: S1, preparation Euphausia Superba sample;S2, Euphausia Superba sample is pretreated, Euphausia Superba sample is immersed in the cleaning fluid prepared in advance, and stationary for a certain time;S3, ultrasonic cleaning device is used to Euphausia Superba sample is treated with ultrasonic cleaning, under the action of ultrasonic, the shell of Euphausia Superba sample and meat body are separated;S4, the meat body and shell separated are respectively fished from cleaning fluid.The shell meat separation method of the application carries out the separation processing of shell and meat body to Euphausia Superba, can realize shell meat interface accurate dissociation on microscale, while maximum limit retains muscle tissue activity, and it is very simple, efficient, low in cost to operate.

Description

Technical Field

[0001] This invention relates to a krill shell and meat separation technology, and more particularly to an ultrasound-assisted method for separating Antarctic krill shell and meat. Background Technology

[0002] With the deepening development and utilization of Antarctic krill resources, the demand for high-purity tissue samples in the scientific research field has increased dramatically. For example, studying the expression of krill muscle-specific proteins requires avoiding interference from shell-derived chitin or pigments; studying the diet and response to environmental changes of krill at different growth and development stages requires intact and clean shells and meat. However, the high adhesion between the shell and muscle tissue of Antarctic krill, its small size (typically 3-6 cm in length), and the fragility of its shell make shell-meat separation a key technical bottleneck restricting the efficient extraction of active ingredients, molecular biology and ecological research, and precise analysis in the laboratory.

[0003] Currently, the separation of shell and meat in Antarctic krill mainly relies on traditional manual peeling, mechanical crushing and screening, enzymatic hydrolysis, or temperature difference methods, all of which have significant drawbacks. For example, the traditional manual peeling method depends on the experience of the experimenter, and the processing time for a single krill is at least 5 minutes. Moreover, the separation of shell and meat is incomplete, with a high rate of shell fragment residue (5-15%) in the muscle tissue. Although mechanical crushing and screening can improve the processing speed, it is difficult to achieve precise separation of shell and muscle. Enzymatic hydrolysis uses proteases (such as trypsin) to degrade the shell-meat connecting tissue, but it suffers from problems such as high enzyme costs, long reaction time (several hours), residual enzymes interfering with subsequent detection and analysis (such as mass spectrometry), and limited damage to the chitinous structure of the inner shell, resulting in unstable separation effects. The temperature difference method is energy-intensive and easily leads to meat shrinkage and deterioration, interfering with subsequent detection and analysis.

[0004] In summary, the problem that this invention aims to solve is:

[0005] The aim is to find a method that can achieve precise dissociation of the shell-meat interface at the microscale while preserving the activity of muscle tissue to the maximum extent. Summary of the Invention

[0006] The purpose of this invention is to provide an ultrasound-assisted method for separating the shell and meat of Antarctic krill, which enables precise separation of the shell and meat from the krill.

[0007] To achieve the above-mentioned technical objectives, the present invention adopts the following technical solution:

[0008] An ultrasound-assisted method for separating the shell and meat of Antarctic krill, the method comprising:

[0009] S1, Prepare Antarctic krill samples;

[0010] S2, Pre-treat the Antarctic krill samples by immersing them in a pre-prepared cleaning solution and letting them stand for a certain period of time.

[0011] S3. The Antarctic krill sample was cleaned using an ultrasonic cleaning device. Under the action of ultrasound, the shell and flesh of the Antarctic krill sample were separated.

[0012] S4, the separated flesh and shell are retrieved from the cleaning solution.

[0013] Furthermore, the cleaning solution is a functional cleaning solution, which is a mixed solution of NaCl and EDTA, and the temperature of the cleaning solution is controlled within the range of 4-10℃.

[0014] Furthermore, the collected Antarctic krill samples were categorized into three body length grades: small, medium, and large.

[0015] in,

[0016] Antarctic krill classified as small in body length have a body length range of >2 and ≤3 cm.

[0017] Antarctic krill classified as medium body length ranges from >3 to ≤4 cm in length.

[0018] Antarctic krill classified as large body length ranges from >4 to ≤6 cm;

[0019] The concentration gradient of EDTA in the cleaning solution is adjusted according to the body size grades of Antarctic krill.

[0020] For Antarctic krill of small body length, the concentration gradient of EDTA in the washing solution was set at 0.01% EDTA.

[0021] For Antarctic krill of medium body length, the concentration gradient of EDTA in the washing solution was set at 0.03% EDTA.

[0022] For Antarctic krill of a certain size, the concentration gradient of EDTA in the cleaning solution was set to 0.05% EDTA.

[0023] The concentration of NaCl in the cleaning solution is 0.1%.

[0024] Furthermore, the collected Antarctic krill samples were categorized into three body length grades: small, medium, and large.

[0025] in,

[0026] Antarctic krill classified as small in body length have a body length range of >2 and ≤3 cm.

[0027] Antarctic krill classified as medium body length ranges from >3 to ≤4 cm in length.

[0028] Antarctic krill classified as large body length ranges from >4 to ≤6 cm;

[0029] For Antarctic krill of small body length, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 40kHz, the power density to 60-80W / L, the cleaning time to 2-3 minutes, and the cleaning temperature to 10-12℃.

[0030] For medium-length Antarctic krill, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 30kHz, the power density to 80-100W / L, the cleaning time to 3-4 minutes, and the cleaning temperature to 8-10℃.

[0031] For Antarctic krill of similar body length, the ultrasonic cleaning device was set to an ultrasonic frequency of 20kHz, a power density of 100-120W / L, a cleaning time of 4-5 minutes, and a cleaning temperature of 4-5℃.

[0032] Furthermore, when retrieving the meat and shell from the cleaning solution, a screen device is used to separate the meat and shell from the cleaning solution.

[0033] Furthermore, the shell-meat separation method further includes:

[0034] S5, Evaluation of the effectiveness of the method implementation, specifically including:

[0035] When preparing Antarctic krill samples, all Antarctic krill samples were counted to obtain the total number of samples. All Antarctic krill samples were weighed and their total weight was obtained. ;

[0036] After retrieving the separated flesh and shell from the cleaning solution, the retrieved shells are counted to obtain the number of sample shells. The retrieved meat was weighed and its weight was recorded to obtain the sample weight. ;

[0037] The shell integrity rate is calculated using the formula. and meat recycling rate ,

[0038] Shell integrity rate The calculation formula is:

[0039]

[0040] Meat recycling rate The calculation formula is:

[0041]

[0042] In the formula, This indicates the muscle percentage of Antarctic krill, which averages 35-40% in laboratory tests.

[0043] Based on the calculated shell integrity rate and meat recycling rate The numerical value is used to quantify the effectiveness of the method implementation.

[0044] The advantages of the ultrasound-assisted Antarctic krill shell-meat separation method of the present invention compared with the prior art are as follows:

[0045] The shell-meat separation method of the present invention is used to separate the shell and meat of Antarctic krill. It can achieve precise separation of the shell-meat interface at the microscopic scale, while maximizing the preservation of muscle tissue activity. It is also very simple to operate, highly efficient and low in cost. Attached Figure Description

[0046] Figure 1 This is a schematic flowchart of the ultrasound-assisted Antarctic krill shell and meat separation method of the present invention. Detailed Implementation

[0047] The following provides further details on specific embodiments of the present invention:

[0048] This embodiment provides an ultrasound-assisted method for separating the shell and meat of Antarctic krill. This method achieves efficient separation and precise quantification of the shell and meat in a laboratory setting by optimizing parameters and standardizing operating procedures, thereby meeting the reliability requirements of scientific research data.

[0049] The most important innovative feature of the shell-meat separation method in this embodiment is that it utilizes ultrasonic dynamic parameter matching (20-40 kHz) and EDTA (ethylenediaminetetraacetic acid) gradient chelation technology, combined with shell integrity rate... and meat recycling rate The calculation formula enables rapid, low-loss separation and accurate quantitative analysis.

[0050] See Figure 1 The shell-meat separation method of this embodiment specifically includes the following steps S1 to S5.

[0051] S1. Prepare Antarctic krill samples, specifically including the following steps S11 to S13.

[0052] S11, collect fresh Antarctic krill samples, and remove damaged individuals from the collected Antarctic krill samples.

[0053] S12, data statistics were performed on all Antarctic krill samples, including:

[0054] All Antarctic krill samples were counted and statistically analyzed to obtain the total number of samples. This represents the total number of samples;

[0055] All Antarctic krill samples were weighed and their total weight was calculated. The total weight of the sample is expressed as ±1 mg (the data obtained is accurate to ±1 mg).

[0056] S13. For the collected Antarctic krill samples, the body length is classified into different grades.

[0057] In this embodiment, the collected Antarctic krill samples were divided into three body length categories: small, medium, and large.

[0058] in,

[0059] Antarctic krill classified as small in body length have a body length range of >2 and ≤3.

[0060] Antarctic krill classified as medium body length ranges from >3 to ≤4 cm in length.

[0061] Antarctic krill classified as large krill have a body length range of >4 and ≤6.

[0062] The unit for the body length data above is centimeters (cm).

[0063] S2, Preprocessing of the Antarctic krill sample prepared in step S1, including steps S21 to S22.

[0064] S21. Prepare a cleaning solution for cleaning Antarctic krill samples in advance. The cleaning solution is a functional cleaning solution, specifically a mixed solution of 0.1% NaCl + gradient EDTA. The temperature of the cleaning solution is controlled within the range of 4-10℃.

[0065] It should be noted that the concentration gradient of EDTA in the cleaning solution is adjusted according to the body length grades of Antarctic krill. Specifically,

[0066] For Antarctic krill of small body length, the concentration gradient of EDTA in the washing solution was set at 0.01% EDTA.

[0067] For Antarctic krill of medium body length, the concentration gradient of EDTA in the washing solution was set at 0.03% EDTA.

[0068] For Antarctic krill of the appropriate body length, the concentration gradient of EDTA in the cleaning solution was set to 0.05% EDTA.

[0069] The functional cleaning solution is characterized by: chelating calcium and magnesium ions in the shell membrane, directionally breaking the chitin-collagen cross-linking structure, and forming microcracks at the interface to protect the integrity of the muscle cell membrane and the bioactivity of the muscle.

[0070] S22. Immerse the Antarctic krill sample in a pre-prepared functional cleaning solution and let it stand for 10 minutes. The purpose is to precisely create micro-cracks at the shell-meat interface to maximize the preservation of muscle freshness and cell integrity.

[0071] Specifically

[0072] In the cleaning solution, EDTA, as a potent multidentate chelating agent, binds to Ca²⁺ and Mg²⁺ in the krill shell membrane, disrupting the cross-linked structure of the chitin-collagen complex and significantly improving shell integrity. Simultaneously, EDTA inhibits free radical generation (Fenton reaction) by chelating Fe²⁺ / Cu²⁺, reducing lipid peroxidation value by 50-70% and ensuring the integrity of active muscle components.

[0073] S3. For the Antarctic krill samples that have undergone pretreatment in step S2, use a pre-equipped ultrasonic cleaning device to perform ultrasonic cleaning on the Antarctic krill samples.

[0074] It should be noted that the ultrasonic cleaning device is a prior art device, and those skilled in the art will understand that the ultrasonic cleaning device includes an ultrasonic cleaning tank, a mesh basket, and other components.

[0075] Specifically

[0076] The cleaning solution, along with the Antarctic krill sample, was poured into the ultrasonic cleaning tank of the ultrasonic cleaning device (the Antarctic krill sample was placed in a net basket).

[0077] Then, different ultrasonic cleaning device parameters were set for Antarctic krill samples of different body lengths.

[0078] Specifically

[0079] For Antarctic krill of small body length, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 40kHz, the power density to 60-80W / L, the cleaning time to 2-3 minutes, and the cleaning temperature to 10-12℃.

[0080] For medium-length Antarctic krill, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 30kHz, the power density to 80-100W / L, the cleaning time to 3-4 minutes, and the cleaning temperature to 8-10℃.

[0081] For Antarctic krill of general length, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 20kHz, the power density to 100-120W / L, the cleaning time to 4-5 minutes, and the cleaning temperature to 4-5℃.

[0082] Then, the ultrasonic cleaning device is turned on to perform ultrasonic cleaning on the Antarctic krill sample. Under the action of ultrasound, the shell of the Antarctic krill sample separates from the flesh, thus achieving molting.

[0083] During the cleaning process, the net basket can be manually shaken to prevent the Antarctic krill samples from piling up.

[0084] After all the Antarctic krill samples had molted, the ultrasonic cleaning device was turned off, thus completing the ultrasonic cleaning process for the Antarctic krill samples.

[0085] It should be noted that,

[0086] For Antarctic krill of small body length, an ultrasonic frequency of 40kHz was selected. This allows the high-frequency cavitation effect to precisely disrupt the thin-shell connective tissue.

[0087] The 30 kHz ultrasonic frequency is beneficial for balancing penetration and cavitation intensity, making it suitable for medium-length Antarctic krill.

[0088] The 20 kHz ultrasonic frequency is beneficial for enhancing penetration of the thick shells of Antarctic krill of large body length, reducing power and preventing damage.

[0089] S4. After completing the ultrasonic cleaning of the Antarctic krill samples, a pre-prepared sieve device was used to separate the meat and shell in the cleaning solution. The separated meat and shell were then taken out of the cleaning solution and subjected to subsequent processing (data statistics).

[0090] Specifically

[0091] First, a primary sieve device (300μm mesh diameter) is used to remove the shell from the cleaning solution.

[0092] Then, a two-stage sieve device (100μm mesh diameter) is used to remove large meat particles from the cleaning solution.

[0093] Finally, a three-stage sieve device (with a mesh diameter of 50μm) is used to remove the loose meat pieces from the cleaning solution.

[0094] When retrieving fragmented meat, low-temperature centrifugation-screening integrated technology can be used to obtain meat tissue.

[0095] The retrieved shells were counted and statistically analyzed to obtain the number of sample shells. To indicate the number of sample shells

[0096] The retrieved meat was weighed and its weight was recorded to obtain the sample weight. This is used to represent the weight of the sample body.

[0097] S5, Evaluation of the effectiveness of the method implementation.

[0098] Specifically, based on previously determined data, including the total number of samples... Total sample weight Number of sample shells Sample body weight .

[0099] Calculate the shell integrity rate separately and meat recycling rate ,

[0100] Specifically

[0101] Shell integrity rate The calculation formula is:

[0102]

[0103] Meat recycling rate The calculation formula is:

[0104]

[0105] In the formula, This indicates the percentage of muscle in Antarctic krill, which averages 35-40% in laboratory tests.

[0106] The final calculated shell integrity rate and meat recycling rate In general, the larger the values ​​of both, the better the implementation effect of the shell-meat separation method in this embodiment, and the more thorough the separation of the shell and the meat.

[0107] The shell-meat separation method of this embodiment is used to separate the shell and meat of Antarctic krill. This shell-meat separation method can achieve precise separation of the shell-meat interface at the microscopic scale, while preserving the activity of muscle tissue to the maximum extent. It is also very simple to operate, highly efficient and low in cost, which is conducive to obtaining accurate data related to Antarctic krill experiments.

[0108] Furthermore, the shell-meat separation method of this embodiment also has the following advantages:

[0109] 1) Experimental-grade non-destructive separation: shell integrity rate ≥90%, meat recovery rate ≥85%, meeting the needs of omics analysis;

[0110] 2) Zero chemical pollution: The entire process is enzyme-free and solvent-free, compatible with metabolomics / transcriptomics;

[0111] 3) High-throughput adaptation: Processing 50 samples (2-6 cm) in a single batch takes ≤6 minutes, improving efficiency by 40 times;

[0112] 4) Precise quality assessment: Establish shell integrity rate With meat recycling rate Calculation formulas, standardized data reports;

[0113] 5. Zero hardware modification: Optimize parameters based on existing ultrasonic cleaning equipment to adapt to laboratory resource constraints.

[0114] Here is a specific example:

[0115] In a specific experimental project, 50 Antarctic krill (medium body length grade) were tested, and the results are shown in the table below:

[0116]

[0117] The above are merely preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for separating the shell and meat of Antarctic krill based on ultrasound assistance, characterized in that: The shell-meat separation method includes: S1, Prepare Antarctic krill samples; S2, Pre-treat the Antarctic krill samples by immersing them in a pre-prepared cleaning solution and letting them stand for a certain period of time. S3. The Antarctic krill sample was cleaned using an ultrasonic cleaning device. Under the action of ultrasound, the shell and flesh of the Antarctic krill sample were separated. S4, the separated flesh and shell are taken out of the cleaning solution respectively; The cleaning solution is a functional cleaning solution, which is a mixed solution of NaCl and EDTA, and the temperature of the cleaning solution is controlled within the range of 4-10℃. The collected Antarctic krill samples were classified into three body length categories: small, medium, and large. in, Antarctic krill classified as small in body length have a body length range of >2 and ≤3 cm. Antarctic krill classified as medium body length ranges from >3 to ≤4 cm in length. Antarctic krill classified as large body length ranges from >4 to ≤6 cm; The concentration gradient of EDTA in the cleaning solution is adjusted according to the body size grades of Antarctic krill. For Antarctic krill of small body length, the concentration gradient of EDTA in the washing solution was set at 0.01% EDTA. For Antarctic krill of medium body length, the concentration gradient of EDTA in the washing solution was set at 0.03% EDTA. For Antarctic krill of a certain size, the concentration gradient of EDTA in the cleaning solution was set to 0.05% EDTA. The concentration of NaCl in the cleaning solution is 0.1%; For Antarctic krill of small body length, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 40kHz, the power density to 60-80W / L, the cleaning time to 2-3 minutes, and the cleaning temperature to 10-12℃. For medium-length Antarctic krill, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 30kHz, the power density to 80-100W / L, the cleaning time to 3-4 minutes, and the cleaning temperature to 8-10℃. For Antarctic krill of general length, the ultrasonic frequency parameters of the ultrasonic cleaning device are set to 20kHz, the power density to 100-120W / L, the cleaning time to 4-5 minutes, and the cleaning temperature to 4-5℃. For small-sized Antarctic krill, a 40kHz ultrasonic frequency is selected, as the high-frequency cavitation effect precisely disrupts the thin shell connecting tissue. A 30kHz ultrasonic frequency is beneficial for balancing penetration power and cavitation intensity, making it suitable for medium-sized Antarctic krill. A 20kHz ultrasonic frequency is beneficial for enhancing penetration of the thick shell of large-sized Antarctic krill, reducing power and preventing damage.

2. The method for separating Antarctic krill shell and meat based on ultrasound assistance according to claim 1, characterized in that: When retrieving the meat and shell from the cleaning solution, a screen device is used to separate the meat and shell from the cleaning solution.

3. The method for separating Antarctic krill shell and meat based on ultrasound assistance according to claim 1, characterized in that: The shell-meat separation method further includes: S5, Evaluation of the effectiveness of the method implementation, specifically including: When preparing Antarctic krill samples, all Antarctic krill samples were counted to obtain the total number of samples. All Antarctic krill samples were weighed and their total weight was obtained. ; After retrieving the separated flesh and shell from the cleaning solution, the retrieved shells are counted to obtain the number of sample shells. The retrieved meat was weighed and its weight was recorded to obtain the sample weight. ; The shell integrity rate is calculated using the formula. and meat recycling rate , Shell integrity rate The calculation formula is Meat recycling rate The calculation formula is In the formula, This indicates the muscle percentage of Antarctic krill, which averages 35-40% in laboratory tests. Based on the calculated shell integrity rate and meat recycling rate The numerical value is used to quantify the effectiveness of the method implementation.

Images