Biological cell culture container

Abstract

The present invention relates to the technical field of microbial culture, and discloses a biological cell culture container, which comprises: a plate body, which is provided with a plurality of base holes, and is provided with a fluid channel between two adjacent base holes in each column; a liquid transfer channel is arranged between the bottom of the base hole and the upper plate surface of the plate body on the periphery of the hole opening of the base hole, and the liquid transfer channel penetrates through the upper plate surface of the plate body to form a liquid transfer port; vertically extending hole cavities are formed in the fluid channels on the two sides of the base hole, and the stopping rods are inserted into the hole cavities to close the fluid channels; a liquid transfer member that sucks the culture medium from or re-feeds the culture medium into the base hole by means of the liquid transfer port and through the liquid transfer channel; and after the culture medium in the basal hole is transferred, the cell transfer tube extends into the bottom of the basal hole to pick up cells.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, in particular to a biological cell culture container. Background Art

[0002] In the prior art, a typical container capable of simultaneously culturing a large number of biological cells is a well plate, which specifically includes a plate body and rectangularly arranged basic holes arranged on the plate body. The biological cells are arranged in the basic holes and cultured with the culture medium therein. In order to be able to replace or transfer the culture medium as uniformly as possible, fluid channels are opened between each column of the basic holes to enable communication between adjacent basic holes. In this way, the culture medium can flow along the fluid channels to achieve the replacement or transfer of the culture medium, and interception structures are arranged on both sides of each basic hole to limit the biological cells from moving to other basic holes with the flow of the culture medium.

[0003] When culturing biological cells using well plates, there may be a requirement to transfer cells from one (or some) of the wells. However, the presence of a large amount of culture medium in the wells makes it difficult to capture or pick up the cells in the culture medium, thus making the transfer operation difficult. Currently, the following two methods are mainly used in the existing technology to achieve cell transfer.

[0004] The first method involves pre-placing a filter basket in each culture medium. When cells need to be transferred from the basal wells, the filter basket is lifted so that only its bottom is in the culture medium. This helps limit the free range of cells, facilitating their capture or pickup using a transfer tube. However, this method has two drawbacks: First, the filter basket is typically fixed to the well plate. For example, the filter basket is often bonded to the well plate to ensure its stability. However, if the filter basket is lifted using tools (such as tweezers), it cannot be re-secured and thus becomes difficult to stabilize in the basal wells. Second, when the filter basket, which is typically made of a mesh and filter cloth, is lifted, cells may adhere to the basket and become difficult to locate in the culture medium at the bottom of the basket, making it difficult to pick up the cells or easily damaging them during pickup.

[0005] The second method is to temporarily discharge the culture medium in the column where the base wells are located so that the cells to be picked up can only float freely in a smaller range, thereby facilitating the picking of cells. However, this method is bound to affect the culture of cells in other base wells, and after picking up the cells, a large amount of culture medium needs to be re-injected into the base wells. Summary of the Invention

[0006] In view of the above technical problems existing in the prior art, the present invention provides a biological cell culture container.

[0007] In order to solve the above technical problems, the technical solution adopted by the present invention is: A biological cell culture container, comprising: A plate body is provided with a plurality of rectangularly arranged basic holes, a fluid channel is provided between two adjacent basic holes in each row, and interception structures are provided between both sides of each basic hole and the fluid channel for allowing culture medium to pass through while restricting the passage of cells; a liquid transfer channel is provided between the bottom of the basic hole and the upper plate surface of the plate body at the periphery of the orifice of the basic hole, and the liquid transfer channel extends through the upper plate surface of the plate body to form a liquid transfer port; Stop rods, wherein vertically extending cavities are provided in the fluid channels on both sides of the base hole, and the stop rods are inserted into the cavities to close the fluid channels; a liquid transfer component for aspirating a culture medium from the base well or resupplying the culture medium into the base well via the liquid transfer port and the liquid transfer channel; The cell transfer tube extends to the bottom of the base well to pick up cells after the culture medium in the base well is transferred.

[0008] Preferably, the liquid transfer component comprises an inner cylinder and an outer cylinder, wherein the inner cylinder and the outer cylinder are sealed at the top and bottom to define an annular cavity, and both the inner cylinder and the outer cylinder are made of bellows to enable the liquid transfer component to elastically expand and contract axially; wherein: The bottom of the liquid transfer component is provided with a liquid through hole that passes through the annular cavity. If the culture medium in the base hole needs to be transferred, the liquid through hole at the bottom of the liquid transfer component is docked with the liquid transfer port, and the culture medium in the base hole is sucked into the annular cavity by axially compressing the liquid transfer component and then resetting it; after the required cells are transferred from the base hole, the culture medium in the annular cavity is re-supplied into the base hole by axially compressing the liquid transfer component.

[0009] Preferably, the biological cell culture container further comprises a rod having a central hole, and the liquid transfer component is attached to the bottom of the rod; wherein: The bottom of the liquid transfer component is provided with a lower ring plate, and the liquid through hole passes through the lower ring plate; two first magnetic pieces extending in the circumferential direction are fixed to the annular area outside the orifice of each base hole, and the two first magnetic pieces are butted together in the circumferential direction, and the two magnetic poles of the two first magnetic pieces are both located at the two ends in the circumferential direction, and the magnetic poles of the butted ends of the two first magnetic pieces are the same, so that the magnetic poles of the non-butted ends of the two first magnetic pieces are the same; a second magnetic piece is fixed to the bottom of the lower ring plate, and the two magnetic poles of the second magnetic piece are located at the two ends in the circumferential direction; An air guide groove extending radially and extending beyond the annular area is provided on the periphery of the opening of the base hole; After the lower ring plate of the liquid transfer component is brought into contact with the annular area and the liquid through hole is docked with the liquid transfer port by means of the rod, the second magnetic piece is opposed to one of the first magnetic pieces to achieve mutual attraction; After the liquid transfer component is driven by the rod to cause the lower ring plate to twist, the second magnetic piece rotates to be opposite to the other first magnetic piece to achieve mutual repulsion, and after the liquid transfer component is twisted, the liquid through hole is opposite to the radial inner end of the air guide groove, and the external gas is connected to the liquid through hole through the air guide groove.

[0010] Preferably, an upper ring plate is provided on the top of the liquid transfer component, a third magnetic piece is fixed on the upper ring plate, and two magnetic poles of the third magnetic piece are located at two ends in the circumferential direction; a fourth magnetic piece is fixed on the bottom of the rod body, and two magnetic poles of the fourth magnetic piece are located at two ends in the circumferential direction, and the fourth magnetic piece and the third magnet are attracted to each other by making the fourth magnetic piece face the third magnetic piece; wherein: The magnetic attraction between the fourth magnetic piece and the third magnet is made greater than the magnetic attraction between the second magnetic piece and the first magnetic piece.

[0011] Preferably, an annular groove is provided in the annular area, and the annular groove is used for axial positioning of the lower ring plate.

[0012] Preferably, the liquid transfer port is covered with a valve plate made of a flexible material, a central area of the valve plate is provided with circumferentially arranged expandable gaps, and an annular protrusion is arranged around the periphery of the expandable gaps.

[0013] Preferably, the liquid transfer channel includes a first vertical channel, a second vertical channel and a transverse channel, the first vertical channel extends vertically from the bottom of the plate body to the liquid transfer port, the second vertical channel extends vertically from the bottom of the plate body to below the base hole, and the transverse channel is opened on the lower surface of the plate body and extends to the lower ends of the first vertical channel and the second vertical channel respectively; the bottom of the plate body is covered with a bottom plate.

[0014] Preferably, the lower portion of the base hole is configured to be conical, and a plurality of flow gaps arranged circumferentially are opened in the lower portion of the base hole, and the second vertical channel extends to the flow gaps.

[0015] Preferably, the intercepting structure comprises a plurality of grids defining a plurality of fluid gaps.

[0016] Preferably, a hole wall is defined between the fluid channel and the base hole, and a plurality of the grids are processed on the hole wall.

[0017] Compared with the prior art, the biological cell culture container provided by the present invention has the following beneficial effects: The biological cell culture device provided by the present invention makes the transfer operation of cells in certain (certain) base wells simple.

[0018] This disclosure is an overview of various implementations or examples of the technology, and is not a comprehensive disclosure of the full scope or all features of the disclosed technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar components in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar components. The accompanying drawings generally illustrate various embodiments by way of example and not limitation, and together with the description and claims, serve to illustrate the embodiments of the invention. Where appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive of the embodiments of the present apparatus or method.

[0020] Figure 1 This is a schematic diagram of the three-dimensional structure of the biological cell culture container provided by the present invention.

[0021] Figure 2 This is a top view of the plate body in the biological cell culture container provided by the present invention.

[0022] Figure 3 for Figure 2 An enlarged view of detail A.

[0023] Figure 4 for Figure 3 BB cross-sectional view.

[0024] Figure 5 for Figure 4 An enlarged view of a portion C.

[0025] Figure 6 Schematic diagram of the three-dimensional structure of the rod body and the liquid transfer component (showing the bottom of the liquid transfer component).

[0026] Figure 7 Schematic diagram of the three-dimensional structure of the liquid transfer component (showing the top of the liquid transfer component).

[0027] Figure 8 Schematic diagram of the three-dimensional structure of the rod body (showing the bottom of the rod body).

[0028] Figure 9 This is a view of the liquid transfer component in use (the liquid transfer component is in the extended state).

[0029] Figure 10 This is a view of the liquid transfer component in use (the liquid transfer component is in a compressed state).

[0030] Reference numerals: 10-plate body; 11-upstream liquid storage tank; 12-downstream liquid storage tank; 13-base hole; 131-flow gap; 14-fluid channel; 141-upstream expanded arc cavity; 142-downstream expanded arc cavity; 143-upstream interception structure; 144-downstream interception structure; 145-hole; 15-annular sink; 16-air guide groove; 20-liquid transfer channel; 21-first vertical channel; 211-liquid transfer port; 22-second vertical channel; 23-transverse channel; 24-valve plate; 241-expandable gap; 242-annular protrusion; 30-liquid transfer component; 31-outer cylinder; 32-inner cylinder; 33-lower ring plate; 34-upper ring plate; 35-liquid through hole; 40-rod body; 41-first magnetic plate; 42-second magnetic plate; 51-third magnetic plate; 52-fourth magnetic plate; 60-stop rod. DETAILED DESCRIPTION

[0031] Unless otherwise defined, the technical or scientific terms used in the present invention shall have the usual meanings understood by persons of ordinary skill in the field to which the present invention belongs. The words "first", "second" and similar terms used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" mean that the elements or objects preceding the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. Words such as "connect" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0032] In order to keep the following description of the embodiments of the present invention clear and concise, detailed descriptions of known functions and known components are omitted.

[0033] like Figures 1 to 10 As shown, the present invention discloses a biological cell culture container, which includes: a plate body 10, a stop rod 60, a liquid transfer component 30 and a cell transfer tube (not shown in the drawings).

[0034] A plurality of basic holes 13 arranged in a matrix are provided on the upper surface of the plate body 10. Cells are located in the basic holes 13 and cultured with the culture medium therein. A fluid channel 14 is provided between each adjacent two basic holes 13 in each column of basic holes 13. The fluid channel 14 connects all the basic holes 13 in each column in series. An upstream liquid reservoir 11 and a downstream liquid reservoir 12 are provided on both sides of the upper surface of the plate body 10. The upstream liquid reservoir 11 and the downstream liquid reservoir 12 are respectively opposite to the basic holes 13 in all columns. The fluid channels 14 upstream of the basic holes 13 in all columns are connected to the upstream liquid reservoir 11, and the fluid channels 14 downstream of the basic holes 13 in all columns are connected to the downstream liquid reservoir 12. In this way, the culture medium can flow from the upstream liquid reservoir 11 through the fluid channels 14 and the basic holes 13 to the downstream liquid reservoir 12 by tilting the plate body 10 or by flowing the culture medium in the downstream liquid reservoir 12 to the upstream liquid reservoir 11, so that there is always culture medium in the basic holes 13 and the culture medium keeps flowing.

[0035] like Figure 3 and Figure 4 As shown, an upstream interception structure 143 is configured between the fluid channel 14 upstream of each basal pore 13 and the basal pore 13, and a downstream interception structure 144 is configured between the fluid channel 14 downstream and the basal pore 13, so as to limit the cells in the basal pore 13 from transferring to other basal pores 13 through the fluid channel 14. Preferably, the upstream interception structure 143 and the downstream interception structure 144 are both formed by alternating arrangement of fluid slits and grids processed on the wall of the basal pore 13. In order to prevent the interception structure from throttling the culture medium, an upstream expanded arc-shaped cavity 141 extending in the circumferential direction is processed upstream of the upstream interception structure 143, and a downstream expanded arc-shaped cavity 142 extending in the circumferential direction is processed downstream of the downstream interception structure 144, so as to increase the flow cross-section allowing fluid to pass through by increasing the number of fluid slits arranged.

[0036] In the present invention, Figure 3 As shown, the fluid channel 14 upstream and the fluid channel 14 downstream of each base hole 13 are provided with a cavity 145 whose radial dimension is greater than the width of the fluid channel 14, and the radial dimension of the stop rod 60 is roughly equal to the radial dimension of the cavity 145. In this way, the upstream and downstream fluid channels 14 are closed by inserting two stop rods 60 into the cavities 145 upstream and downstream of the base hole 13 using a tool such as tweezers, thereby preventing the culture medium in other base holes 13 and fluid channels 14 from being transferred to the base hole 13 between the two stop rods 60.

[0037] In the present invention, Figure 4As shown, a liquid transfer channel 20 is constructed between the bottom of the base hole 13 and the upper plate surface of the plate body 10 at the periphery of the orifice of the base hole 13, and the liquid transfer channel 20 is connected to the upper plate surface of the plate body 10 to form a liquid transfer port 211. In this way, when it is necessary to transfer the culture medium in a certain base hole 13 or to re-supply the culture medium into the base hole 13, first, two stop rods 60 are inserted into the upstream and downstream cavities 145 of the base hole 13, and then, the culture medium can be sucked from the base hole 13 or re-supplyed into the base hole 13 through the liquid transfer component 30 (to be described below) with the help of the liquid transfer port 211 and through the liquid transfer channel 20.

[0038] like Figure 4 As shown, the liquid transfer channel 20 specifically includes a first vertical channel 21, a second vertical channel 22, and a transverse channel 23. The first vertical channel 21 extends vertically from the bottom of the plate body 10 to the liquid transfer port 211, and the second vertical channel 22 extends vertically from the bottom of the plate body 10 to below the base hole 13. The transverse channel 23 is opened on the lower surface of the plate body 10 and extends to the lower ends of the first vertical channel 21 and the second vertical channel 22 respectively, and the bottom of the plate body 10 is covered by the bottom plate. The above-mentioned liquid transfer channel 20 is easy to process and form on the plate body 10.

[0039] The base hole 13 is preferably configured as follows: the lower portion of the base hole 13 is configured with a tapered structure and is provided with numerous circumferentially arranged flow slits 131. The liquid transfer channel 20 (e.g., the second vertical channel 22) extends through the flow slits 131. Thus, as the liquid transfer component 30 aspirates the culture medium within the base hole 13, the liquid level within the base hole 13 drops to the lower portion of the base hole 13, confining the cells to a smaller active area, thereby facilitating cell collection using a cell transfer tube.

[0040] A cell transfer tube in the prior art is selected as the cell transfer tube of the present invention (not shown in the drawings). The cell transfer tube is used to suck cells into the tube holes manually or by external equipment by forming negative pressure inside the tube.

[0041] like Figures 6 to 10 Combined with Figure 3As shown, the present invention provides a liquid transfer component 30 that operates better than instruments such as syringes. The liquid transfer component 30 comprises an inner tube 32 and an outer tube 31. The outer tube 31 is sleeved over the inner tube 32. The top and bottom of the outer tube 31 and the inner tube 32 are both sealed to define an annular cavity. The outer tube 31 and the inner tube 32 are constructed of bellows, allowing the liquid transfer component 30 to expand and contract axially. After axial compression and the removal of the axial force, the liquid transfer component 30 automatically extends and returns to its original position. A lower ring plate 33 is fixed to the bottom of the outer tube 31 and the inner tube 32 to strengthen the bottom of the liquid transfer component 30. The lower ring plate 33 is provided with a liquid through hole 35 that extends into the annular cavity. Thus, compression of the liquid transfer component 30 allows fluid in the annular cavity to be discharged through the liquid through hole 35, and resetting and extending the liquid transfer component 30 allows fluid to be drawn into the annular cavity through the liquid through hole 35. An annular groove 15 is machined around the opening of the base hole 13 to position the lower ring plate 33 and limit radial movement of the lower ring plate 33. The liquid transfer port 211 passes through the bottom of the annular groove 15.

[0042] In order to facilitate the operation of the liquid transfer component 30, the present invention also equips the above-mentioned liquid transfer component 30 with a rod body 40. The top of the above-mentioned liquid transfer component 30 is attached to the bottom of the rod body 40. In this way, the liquid transfer component 30 can be moved, axially compressed, and released by holding the rod body 40.

[0043] When it is necessary to transfer the culture medium in the base hole 13, the rod body 40 is held and the lower part of the liquid transfer component 30 is brought close to the base hole 13, and the liquid through hole 35 of the liquid transfer component 30 is aligned with the liquid transfer port 211 at the bottom of the annular trough 15 (the liquid through hole 35 and the liquid transfer port 211 can be quickly aligned by arranging markings on the edge of the annular trough 15 and the lower part of the outer cylinder 31 of the liquid transfer component 30 and aligning the markings), and then the lower ring plate 33 is positioned in the annular trough 15. Then, as shown in FIG. Figure 10 As shown, the rod body 40 is used to axially compress the liquid transfer component 30, and the gas in the annular cavity of the liquid transfer component 30 is discharged through the liquid transfer channel 20, the base hole 13, the inner cylinder 32 and finally from the central hole of the rod body 40. Then, as shown in FIG. Figure 9 As shown, the rod body 40 is used to release the liquid transfer component 30 so that the liquid transfer component 30 is reset and extended, thereby sucking the culture medium in the base hole 13 through the liquid transfer channel 20 and the cells are intercepted by the lower part of the base hole 13. Subsequently, the rod body 40 is used to make the liquid transfer component 30 leave the base hole 13, and then the cells are transferred using the cell transfer tube.

[0044] When the culture medium in the liquid transfer component 30 needs to be re-supplied into the culture medium, the liquid through hole 35 of the liquid transfer component 30 is realigned with the liquid transfer port 211, and then the liquid transfer component 30 is axially compressed again to supply the culture medium in the liquid transfer component 30 into the culture medium. Then, the liquid transfer component 30 is removed from the base hole 13 (or removed from the plate body 10) under the premise of ensuring that the culture medium in the base hole 13 is not sucked a second time.

[0045] An air guide groove 16 is formed radially outward from the bottom of the annular trough 15 and is located at a different circumferential position from the liquid transfer port 211. The function of the air guide groove 16 is to: when the liquid transfer component 30 needs to be removed from the base hole 13, especially when the liquid transfer component 30 in a compressed state after the culture medium has been re-introduced into the base hole 13 needs to be removed from the base hole 13, the rod 40 can be used to twist the liquid transfer component 30 so that the liquid through hole 35 of the liquid transfer component 30 is rotated from a position opposite the liquid transfer port 211 to a position opposite the radial inner end of the air guide groove 16, thereby allowing external gas to enter the annular cavity through the air guide groove and the liquid through hole 35, thereby reducing the adsorption force of the liquid transfer component 30 on the plate body 10 caused by negative pressure, thereby facilitating the liquid transfer component 30 to leave the base hole 13.

[0046] Two circumferentially extending first magnetic pieces 41 are fixed to the bottom of the annular groove 15, such that the south and north poles of the first magnetic pieces 41 are located at the ends of the circumference rather than on both sides of the thickness. This allows the two first magnetic pieces 41 to butt together in the circumferential direction and have the same magnetic poles at the butting ends. For example, both butting ends have south poles. Similarly, the non-butting ends of the two first magnetic pieces 41 also have the same magnetic poles. For example, both non-butting ends have north poles. Furthermore, the first magnetic pieces 41 do not protrude from the bottom of the annular groove 15. A second magnetic piece 42 is fixed to the lower ring plate 33 at the bottom of the liquid transfer component 30. The second magnetic piece 42 has substantially the same size as the first first magnetic piece 41, and its south and north poles are located at the ends of the circumference rather than on both sides of the thickness. In this way, when the second magnetic piece 42 of the lower ring plate 33 is opposite to the first first magnetic piece 41, the S pole and N pole of the second magnetic piece 42 are respectively opposite to the N pole and S pole of the first magnetic piece 41, so that the first first magnetic piece 41 and the second magnetic piece 42 form a magnetic attraction. When the second magnetic piece 42 of the lower ring plate 33 is opposite to the second first magnetic piece 41, the S pole and N pole of the second magnetic piece 42 are respectively opposite to the S pole and N pole of the first magnetic piece 41, so that the second first magnetic piece 41 and the second magnetic piece 42 form a magnetic repulsion. It can be further known that in the process of switching the second magnetic piece 42 from being opposite to the first first magnetic piece 41 to being opposite to the second first magnetic piece 41 by twisting the liquid transfer component 30, the liquid transfer component 30 switches from being attracted to being repelled. The relationship between the curvature of the first magnetic piece 41 and the torsion angle is configured such that when the liquid transfer component 30 is twisted so that the liquid through hole 35 is opposite to the radial inner end of the air guide groove 16, the first magnet switches from being opposite to the first first magnetic piece 41 to being opposite to the second first magnetic piece 41. The advantage of the cooperation between the first magnet and the second magnet is that: after the liquid transfer component 30 is twisted in order to make the liquid transfer component 30 in a compressed state leave the base hole 13 after the culture medium has been re-supplied into the base hole 13, the magnetic repulsion between the first magnetic piece 41 and the second magnetic piece 42 can immediately overcome the adsorption force of the liquid transfer component 30 on the plate body 10 due to the negative pressure, so that it is not necessary to use the air guide groove 16 to completely reset the liquid transfer component 30 before facilitating the liquid transfer component 30 to leave. Therefore, by cooperating with the air guide groove 16, the liquid transfer component 30 can be easily twisted and smoothly leave the base hole 13. More importantly, it is not necessary to twist it exactly to the angle relative to the liquid through hole 35 and the air guide groove 16, but it is only necessary to make the liquid through hole 35 leave the base hole 13 with the help of the magnetic repulsion at the moment when it passes through the air guide groove 16.

[0047] In some preferred embodiments, the top of the transfer component is provided with an upper ring plate 34, on which a third magnetic piece 51 is fixed, with two magnetic poles of the third magnetic piece 51 located at two ends of the circumference. A fourth magnetic piece 52 is fixed to the bottom of the rod body 40, with two magnetic poles of the fourth magnetic piece 52 located at two ends of the circumference. By arranging the fourth magnetic piece 52 and the third magnetic piece 51 opposite each other, the fourth magnetic piece 52 and the third magnet are attracted to each other, so that the magnetic attraction between the fourth magnetic piece 52 and the third magnet is greater than the magnetic attraction between the second magnetic piece 42 and the first magnetic piece 41. The third magnetic piece 51 and the fourth magnetic piece 52 cooperate to facilitate detachable attachment of the liquid transfer component 30 to the rod body 40 and prevent relative rotation between the rod body 40 and the liquid transfer component 30 when the liquid transfer component 30 is twisted.

[0048] In some preferred embodiments, the liquid transfer port 211 is covered with a valve plate 24 made of a flexible material. A central region of the valve plate 24 defines a circumferentially arranged expandable slit 241. An annular protrusion 242 is disposed around the periphery of the expandable slit 241 and is located radially inward of the liquid transfer port 211. The expandable slit 241 deforms to open during medium transfer, allowing medium to pass through. When medium is not being transferred, the expandable slit 241 returns to its original position and closes, providing a sealing effect. The annular protrusion 242 on the flexible valve plate 24 serves to provide a sealing effect when the liquid through hole 35 is mated with the liquid transfer port 211. Furthermore, when the liquid transfer member 30 is twisted, the annular protrusion 242 deforms downward, substantially preventing the twisting of the liquid transfer member 30.

[0049] Furthermore, although exemplary embodiments have been described herein, the scope of the present invention includes any and all embodiments based on the present invention with equivalent elements, modifications, omissions, combinations (e.g., solutions that intersect various embodiments), adaptations, or changes. The elements of the claims are to be interpreted broadly based on the language employed in the claims and are not limited to the examples described in this specification or during the prosecution of this application, which examples are to be construed as non-exclusive. Therefore, it is intended that this specification and examples be considered merely as examples, with the true scope and spirit being indicated by the following claims and their full scope of equivalents.

[0050] The above description is intended to be illustrative and not restrictive. For example, the above examples (or one or more versions thereof) may be used in combination with each other. For example, a person of ordinary skill in the art may use other embodiments when reading the above description. In addition, in the above detailed description, various features may be grouped together to simplify the invention. This should not be interpreted as an intention that a disclosed feature that is not claimed for protection is essential to any claim. On the contrary, the subject matter of the present invention may have less than all the features of a particular disclosed embodiment. Therefore, the following claims are incorporated into the detailed description as examples or embodiments, with each claim independently serving as a separate embodiment, and it is contemplated that these embodiments may be combined with each other in various combinations or arrangements. The scope of the invention should be determined with reference to the appended claims and the full scope of equivalents to which such claims are entitled.

[0051] The above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the scope of the present invention. The scope of protection of the present invention is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the spirit and scope of protection of the present invention, and such modifications or equivalent substitutions shall also be deemed to fall within the scope of protection of the present invention.

Claims

1. A biological cell culture container, characterized in that: include: A plate body is provided with a plurality of rectangularly arranged basic holes, a fluid channel is provided between two adjacent basic holes in each row, and interception structures are provided between both sides of each basic hole and the fluid channel for allowing culture medium to pass through while restricting the passage of cells; a liquid transfer channel is provided between the bottom of the basic hole and the upper plate surface of the plate body at the periphery of the orifice of the basic hole, and the liquid transfer channel extends through the upper plate surface of the plate body to form a liquid transfer port; Stop rods, wherein vertically extending cavities are provided in the fluid channels on both sides of the base hole, and the stop rods are inserted into the cavities to close the fluid channels; a liquid transfer component for aspirating a culture medium from the base well or resupplying the culture medium into the base well via the liquid transfer port and the liquid transfer channel; The cell transfer tube extends to the bottom of the base well to pick up cells after the culture medium in the base well is transferred.

2. The biological cell culture container according to claim 1, characterized in that: The liquid transfer component comprises an inner cylinder and an outer cylinder, wherein the inner cylinder and the outer cylinder are sealed at the top and bottom to define an annular cavity, and the inner cylinder and the outer cylinder are both made of bellows to enable the liquid transfer component to elastically expand and contract axially; wherein: The bottom of the liquid transfer component is provided with a liquid through hole that passes through the annular cavity. If the culture medium in the base hole needs to be transferred, the liquid through hole at the bottom of the liquid transfer component is docked with the liquid transfer port, and the culture medium in the base hole is sucked into the annular cavity by axially compressing the liquid transfer component and then resetting it; after the required cells are transferred from the base hole, the culture medium in the annular cavity is re-supplied into the base hole by axially compressing the liquid transfer component.

3. The biological cell culture container according to claim 2, characterized in that: The biological cell culture container further comprises a rod having a central hole, and the liquid transfer component is attached to the bottom of the rod; wherein: The bottom of the liquid transfer component is provided with a lower ring plate, and the liquid through hole passes through the lower ring plate; two first magnetic pieces extending in the circumferential direction are fixed to the annular area outside the orifice of each base hole, and the two first magnetic pieces are butted together in the circumferential direction, and the two magnetic poles of the two first magnetic pieces are both located at the two ends in the circumferential direction, and the magnetic poles of the butted ends of the two first magnetic pieces are the same, so that the magnetic poles of the non-butted ends of the two first magnetic pieces are the same; a second magnetic piece is fixed to the bottom of the lower ring plate, and the two magnetic poles of the second magnetic piece are located at the two ends in the circumferential direction; An air guide groove extending radially and extending beyond the annular area is provided on the periphery of the opening of the base hole; After the lower ring plate of the liquid transfer component is brought into contact with the annular area and the liquid through hole is docked with the liquid transfer port by means of the rod, the second magnetic piece is opposed to one of the first magnetic pieces to achieve mutual attraction; After the liquid transfer component is driven by the rod to cause the lower ring plate to twist, the second magnetic piece rotates to be opposite to the other first magnetic piece to achieve mutual repulsion, and after the liquid transfer component is twisted, the liquid through hole is opposite to the radial inner end of the air guide groove, and the external gas is connected to the liquid through hole through the air guide groove.

4. The biological cell culture container according to claim 3, characterized in that: The top of the liquid transfer component is provided with an upper ring plate, on which a third magnetic piece is fixed, with two magnetic poles of the third magnetic piece located at two ends in the circumferential direction; the bottom of the rod body is provided with a fourth magnetic piece, with two magnetic poles of the fourth magnetic piece located at two ends in the circumferential direction, and the fourth magnetic piece and the third magnet are attracted to each other by making the fourth magnetic piece face the third magnetic piece; wherein: The magnetic attraction between the fourth magnetic piece and the third magnet is made greater than the magnetic attraction between the second magnetic piece and the first magnetic piece.

5. The biological cell culture container according to claim 3, characterized in that: An annular groove is provided in the annular area, and the annular groove is used for axial positioning of the lower ring plate.

6. The biological cell culture container according to claim 3, characterized in that: The liquid transfer port is covered with a valve plate made of flexible material. A central area of the valve plate is provided with circumferentially arranged expandable gaps, and an annular protrusion is arranged around the periphery of the expandable gaps.

7. The biological cell culture container according to claim 1, characterized in that: The liquid transfer channel includes a first vertical channel, a second vertical channel and a transverse channel. The first vertical channel extends vertically from the bottom of the plate body to the liquid transfer port, the second vertical channel extends vertically from the bottom of the plate body to below the base hole, and the transverse channel is opened on the lower surface of the plate body and extends to the lower ends of the first vertical channel and the second vertical channel respectively; the bottom of the plate body is covered with a bottom plate.

8. The biological cell culture container according to claim 7, characterized in that: The lower portion of the base hole is configured to be conical, and a plurality of flow gaps arranged circumferentially are opened in the lower portion of the base hole, and the second vertical channel extends to the flow gaps.

9. The biological cell culture container according to claim 1, characterized in that: The intercepting structure includes a plurality of grids defining a plurality of fluid gaps.

10. The biological cell culture container according to claim 9, characterized in that: A hole wall is defined between the fluid channel and the base hole, and a plurality of grids are processed on the hole wall.

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