Sample preservation solution for vagina micro-ecology detection and preparation method of sample preservation solution

The cell structure is fixed by combining paraformaldehyde and glutaraldehyde, and stabilizers such as sodium alginate, Tween-20, β-cyclodextrin are used to solve the instability of cell preservation fluid in vaginal microecology detection, achieving efficient cell morphology maintenance and staining effects, reducing false negative and false positive rates, and improving detection accuracy.

CN120424770APending Publication Date: 2025-08-05SUZHOU TURING MICROBIAL TECH CO LTD
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Patent Information

Application Number
CN202510626542.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-15
Publication Date
2025-08-05

AI Technical Summary

Technical Problem

In the prior art, the cell preservation solution detected by vaginal microecology has problems such as cell accumulation, unstable staining effect, and uneven decolorization time, resulting in high false negative and false positive rates, making it difficult to accurately identify lesion cells.

Method used

The combination of paraformaldehyde and glutaraldehyde was used to fix the cell structure, and sodium alginate, Tween-20 and β-cyclodextrin were added as stabilizers. Teasaponin and ammonium chloride were used to destroy the red blood cells. Cell stability was maintained using buffer to ensure cell morphology and Gram staining effect.

Benefits of technology

Effectively reduce the false negative and false positive rates, keep the cell morphology unchanged, improve the difference between staining stability and microscopic analysis, ensure that the gram-positive properties of bacteria and fungi remain unchanged, and improve detection accuracy.

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Abstract

The invention relates to the technical field of biology, in particular to a sample preservation solution for vagina micro-ecology detection and a preparation method of the sample preservation solution. The invention relates to a sample preservation solution for vaginal micro-ecology detection. The sample preservation solution comprises the following components in percentage by mass: 0.5-0.9% of paraformaldehyde, 0.08-0.12% of glutaraldehyde, 10-18% of a buffer solution, 0.1-0.4% of inositol, 1-10% of a stabilizer, 0.8-5% of a cell treatment agent and the balance of water, the stabilizing agent is prepared from sodium alginate, tween-20 and beta-cyclodextrin. The invention aims to solve the problems of high false negative and false positive rate or difficulty in interpretation caused by liquid-based cell accumulation, unstable subsequent dyeing and decoloration time and the like of a cell preservation solution in the prior art, so that the sample preservation solution for sample cells can keep the forms of cells, protozoa and the like unchanged, and ensures that gram staining negative and positive properties of bacteria and fungi are not changed. The staining and decoloring are relatively stable, the false negative and false positive rate of sample cell detection is effectively reduced, and the sample cell lesion detection and judgment are more accurate.
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Citation Information

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