High performance liquid chromatograph and control method thereof

Abstract

The invention discloses a high performance liquid chromatograph, which comprises a body, a placing box, a sample injection pipe, a lifting mechanism and two groups of fixing mechanisms, each group of fixing mechanism comprises a clamping assembly, a supporting assembly and a shielding assembly, and each group of clamping assembly comprises a mounting box, two vertical plates, two first baffle plates, a sliding plate, two second baffle plates and two clamping plates, the two vertical plates are vertically and fixedly arranged on the bottom face of the mounting box, the two first baffles are vertically and fixedly arranged at the end, away from the mounting plate, of the bottom face of the mounting box, a spring is fixedly arranged between one end of the sliding plate and the mounting box, and the other end of the sliding plate is rotationally connected with a first rolling wheel. A rotating rod is fixedly arranged on the bottom surface of the mounting box, the two clamping plates are rotationally connected to the rotating rod, the ends, close to the sliding plate, of the clamping plates are rotationally connected with second rolling wheels, and the other ends are rotationally connected with third rolling wheels. The invention is used for solving the technical problem that the reagent bottle placed on the chromatograph is easy to topple over.

Description

Technical Field

[0001] The invention belongs to the technical field of chromatographs, and in particular relates to a high performance liquid chromatograph and a control method thereof. Background Art

[0002] High-performance liquid chromatography (HPLC) is a highly efficient separation and analysis instrument based on liquid chromatography technology. It is widely used in chemistry, biology, medicine, environment, food and other fields. The working principle is as follows: the mobile phase in the reservoir is pumped into the system by a high-pressure pump, and the sample solution enters the mobile phase through the injector and is carried into the chromatographic column by the mobile phase. The chromatographic column is equipped with a stationary phase. Due to the different distribution systems of the components of the sample solution in the two phases, when they move relative to each other in the two phases, after repeated adsorption-desorption distribution processes, the components have large differences in movement speed and are separated into individual components. They flow out of the column in turn. After these components are detected by the detector, their sample concentrations are converted into electrical signals and transmitted to the recorder, which is then printed out in the form of a graph.

[0003] Currently, during actual use of the chromatograph, reagent bottles are usually placed in the PCR plate on top of the chromatograph. However, the size of the holes on the PCR plate used to place reagent bottles is fixed, and it is impossible to place reagent bottles of different sizes. Moreover, during the detection process, the traction of the injection system may cause the reagent bottles to fall, thereby affecting the detection process. Summary of the Invention

[0004] In view of the above problems, the present invention provides a high performance liquid chromatograph and a control method thereof.

[0005] To achieve the above-mentioned object, the present invention provides a high-performance liquid chromatograph, comprising a body, a placement box fixedly mounted on the top of the body, a sample injection tube, a lifting mechanism, and two sets of fixing mechanisms, wherein the placement box is hollow inside and has an open top, the sample injection tube is a flexible tube with one end extending into the body and the other end located in the placement box, the lifting mechanism is disposed on the bottom surface of the placement box, a mounting block is fixedly mounted on the lifting mechanism, a mounting plate is symmetrically fixedly mounted on the mounting block, and the two sets of fixing mechanisms are symmetrically disposed on the mounting plate; Each group of the fixing mechanisms includes multiple groups of clamping components, multiple groups of supporting components, and multiple groups of shielding components, and the three correspond to each other one by one. Each group of the clamping components includes a mounting box fixed on the top surface of the mounting plate, two vertical plates, two first baffles, a sliding plate arranged along the length direction of the mounting box, two second baffles, and two clamping plates. The mounting box is hollow inside and is opened on one side away from the mounting plate. The two vertical plates are vertically fixed on the bottom surface of the mounting box, and a gap is left between the two for the sliding plate to pass through. The two first baffles are vertically fixed on the bottom surface of the mounting box at one end away from the mounting plate, and a gap is left between the two for the sliding plate to pass through. The cam is secured to the chassis and has a first end that can be adjusted to fit the needs of the vehicle's operation and to enable the vehicle to move freely in the vehicle. The cam is secured to the chassis and has a first end that can adjust the speed of the vehicle's operation and to allow the vehicle to move freely in the vehicle. The cam is secured to the chassis and has a first end that can adjust the speed of the vehicle's operation and to allow the vehicle to move freely in the vehicle.

[0006] Optionally, the lifting mechanism includes a cylinder, the bottom end of the cylinder is fixed on the bottom surface of the placement box, the cylinder piston rod is upward, and the mounting block is fixed on the top end of the cylinder piston rod.

[0007] Optionally, one end of the clamping plate close to the sliding plate is a straight plate, and the other end is a curved plate.

[0008] Optionally, the support assembly includes a first fixing plate, which is arranged in an "L" shape, with one end of the first fixing plate fixed on the mounting plate and the other end extending below the clamping plate.

[0009] Optionally, a buffer pad is fixed on the first fixing plate.

[0010] Optionally, the shielding assembly includes a second fixed plate vertically fixed on the mounting box, a slider, a connecting plate, and a third baffle located above the clamping plate, the second fixed plate is provided with a sliding groove for the sliding of the slider, the connecting plate passes through the slider and is rotatably connected to it, one end of the connecting plate is fixed to the third baffle, and the other end is provided with a knob.

[0011] Optionally, the placement box is hinged with a cover at the opening, and a handle is fixed on the top surface of the cover.

[0012] A control method for a high performance liquid chromatograph comprises the following steps: S1: Start chromatography software; S2: set parameters; S3: Create instrument method and edit; S4: The operation is divided into manual operation and automatic operation, among which the manual operation is divided into manual injection and manual elution; S5: Automatic operation; S6: View the graph.

[0013] The beneficial effects of the present invention are: 1. The present invention adopts the setting of the clamping component to clamp reagent bottles of different specifications and sizes, which is convenient for better fixing the reagent bottles and preventing the reagent bottles from tipping over during operation, so as not to affect the detection process.

[0014] 2. The present invention can support the bottom end of the reagent bottle by adopting the setting of the support component, so as to support the reagent bottle and prevent the reagent bottle from falling due to its own gravity.

[0015] 3. The present invention adopts the setting of the shielding component to shield the reagent bottle without the bottle cap, thereby reducing the dust from falling into the reagent bottle to avoid affecting the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0018] Figure 2 It is a partial cross-sectional view to show the lifting mechanism and the fixing mechanism.

[0019] Figure 3 It is a partial cross-sectional view to show the fixing assembly.

[0020] Figure 4 It is a schematic diagram to show part of the structure of the support assembly.

[0021] Figure 5 yes Figure 4 Enlarged view of part A in .

[0022] Description of Reference Numerals 1. Main body; 2. Placement box; 21. Cover plate; 22. Handle; 3. Sample injection tube; 4. Lifting mechanism; 41. Cylinder; 42. Mounting block; 43. Mounting plate; 5. Fixing mechanism; 51. Clamping assembly; 511. Mounting box; 5111. Rotating rod; 512. Vertical plate; 513. First baffle; 514. Sliding plate; 5141. First roller; 515. Second baffle; 516. Clamping plate; 517. Spring; 518. Second roller; 519. Third roller; 52. Support assembly; 521. First fixed plate; 522. Buffer pad; 53. Shielding assembly; 531. Second fixed plate; 5311. Slide groove; 532. Slider; 533. Connecting plate; 534. Third baffle; 535. Knob. DETAILED DESCRIPTION

[0023] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can also be implemented in other ways different from those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Reference Figure 1 and Figure 2 A high performance liquid chromatograph comprises a main body 1, a storage box 2, a sample injection tube 3, a lifting mechanism 4, and a fixing mechanism 5. The storage box 2 is fixedly mounted on the top of the main body 1, and the storage box 2 is hollow inside and has an opening at the top. A cover plate 21 is hinged at the opening, and a handle 22 is fixed on the top surface of the cover plate 21. The sample injection tube 3 is a hose, and one end extends into the main body 1 and is connected to the sample injection pump in the main body 1. The other end is located in the storage box 2 and can be inserted into the fixing mechanism 5 for sampling. The lifting mechanism 4 is arranged in the middle position of the bottom surface of the storage box 2, and the fixing mechanism 5 is arranged on the lifting mechanism 4, which can not only adjust the height of the fixing mechanism 5, but also fix the reagent bottle.

[0025] When in use, the cover 21 is opened, and the operator can start the lifting mechanism 4 to raise the fixing mechanism 5, put the reagent bottle into the fixing mechanism 5 for fixing, and then start the lifting mechanism 4 again to return the fixing mechanism 5 to the placement box 2 for easy storage of the reagent bottle.

[0026] Reference Figure 2 Lifting mechanism 4 includes a cylinder 41, which is positioned along the height of storage box 2. The piston rod of cylinder 41 faces upward, with its bottom end fixed to the bottom surface of storage box 2. A mounting block 42 is fixed to the top of the piston rod of cylinder 41, and fixing mechanism 5 is mounted on mounting block 42. When cylinder 41 is activated, the piston rod of cylinder 41 drives fixing mechanism 5 to adjust its height, making it easier for the operator to operate.

[0027] Reference Figure 2-Figure 4 , the mounting blocks 42 are respectively fixed with mounting plates 43 at both ends along the width direction thereof, and the mounting plates 43 are arranged along the length direction of the placement box 2. There are two groups of fixing mechanisms 5, and the two groups of fixing mechanisms 5 are symmetrically fixed on the two mounting plates 43. Each group of fixing mechanisms 5 includes a clamping assembly 51, a support assembly 52, and a shielding assembly 53. The number of clamping assemblies 51, support assemblies 52, and shielding assemblies 53 is multiple groups, and in this embodiment, there are five groups, and they correspond one to one. Each group of clamping assemblies 51 includes an installation box 511, a vertical plate 512, a first baffle 513, a sliding plate 514, a second baffle 515, and a clamping plate 516. The installation box 511 is hollow inside and is opened on one side away from the installation plate 43. One end of the installation box 511 is fixed on the top surface of the installation plate 43. There are two vertical plates 512, each fixed vertically at the center of the bottom surface of the installation box 511, with a gap between them for the sliding plate 514 to pass through. There are two first baffles 513, each fixed vertically at the bottom surface of the installation box 511 at the end away from the installation plate 43, with a gap between them for the sliding plate 514 to pass through. The sliding plate 514 is arranged along the length of the installation box 511 and slides within the installation box 511. One end of the sliding plate 514 passes through the gap between the two vertical plates 512, and a spring 517 is fixed between it and the side wall of the installation box 511 near the installation plate 43. The other end passes through the gap between the two first baffles 513 and is rotatably connected to the first roller 5141. There are two second baffles 515, symmetrically positioned on the sliding plate 514 between the vertical plates 512 and the first baffle 513. One end of each second baffle 515 is fixedly connected to the sliding plate 514 along its length, while the other end is angled away from the sliding plate 514. This allows the two vertical plates 512 to support the two second baffles 515 when the sliding plate 514 slides. There are two sets of clamping plates 516, symmetrically positioned on either side of the sliding plate 514. Rotating rods 5111 are fixed to the bottom surface of the mounting box 511, one on each side of the first baffle 513. The middle portion of each clamping plate 516 is rotatably connected to the two rotating rods 5111. The ends of the clamping plates 516 closest to the sliding plate 514 are straight, while the ends away from the sliding plate 514 are curved. The straight ends of the clamping plates 516 are rotatably connected to second rollers 518, which roll along the second baffle 515. The arc-shaped plate end of the clamping plate 516 is rotatably connected to the third roller 519.

[0028] Before the reagent bottle is placed, the spring 517 is in its original length. During use, the operator places the reagent bottle on the arc-shaped plate end of the two clamping plates 516, so that the first roller 5141 and the two third rollers 519 are respectively in contact with the reagent bottle to achieve clamping and fixing of the reagent bottle. At this time, the sliding plate 514 will be pushed to move toward the mounting plate 43. The first baffle 513 and the second baffle 515 will limit the sliding plate 514, and the spring 517 will also be in a compressed state. After taking out the reagent bottle, the sliding plate 514 will be moved away from the mounting plate 43 under the elastic force of the spring 517, so that the sliding plate 514 can be quickly restored to its original position for reuse. In addition, the provision of the first roller 5141 and the third roller 519 can facilitate the clamping of reagent bottles of different specifications.

[0029] Reference Figure 4 The support assembly 52 includes a first fixing plate 521 and a cushion 522. The first fixing plate 521 is arranged in an "L" shape, with one end of the first fixing plate 521 fixed to the bottom surface of the mounting plate 43 and the other end extending below the curved plate of the clamping plate 516. A cushion 522 is fixed to the top surface of one end of the first fixing plate 521 below the curved plate of the clamping plate 516 to support the reagent bottle.

[0030] Reference Figure 2 and Figure 5 The shielding assembly 53 includes a second fixed plate 531, a slider 532, a connecting plate 533, and a third baffle 534. The second fixed plate 531 is vertically fixed to the top surface of the installation box 511, and a slide groove 5311 is provided on the second fixed plate 531. The slide groove 5311 is arranged along the height direction of the second fixed plate 531. The slider 532 is arranged in an "I" shape, and the slider 532 moves in the slide groove 5311. The connecting plate 533 passes through the slider 532 and is rotatably connected to the slider 532. One end of the connecting plate 533 is fixedly connected to the third baffle 534, and the other end is fixed with a knob 535. The third baffle 534 is located above the clamping plate 516 and is used to shield reagent bottles without bottle caps to prevent the entry of dust and other impurities that affect the detection of samples.

[0031] When a reagent bottle needs to be placed, the operator can move the slider 532 to move the baffle upward, and then turn the knob 535 to make the third baffle 534 vertical, so that the reagent bottle can be placed. Then, the operator turns the knob 535 again to make the third baffle 534 parallel to the top surface of the installation box 511, and moves the slider 532 to the top position of the reagent bottle, so that the opening of the reagent bottle without a bottle cap can be covered to reduce the entry of impurities into the reagent bottle.

[0032] A control method for a high performance liquid chromatograph comprises the following steps: S1: Start chromatography software; S2: set parameters; S3: Create an instrument method and edit it. Select the "Automatic" method template, then click the "Select" tab, enter the UV detector dual-channel wavelength, enter the "Sample Name", "Sample Group Name", "Packaging Material", "Column Code" and "Mobile Phase Information", which will be displayed when the report is output later. Click "UV Zero" to select whether the UV detector will reset the detector value to zero when running the method. Click "Gradient Table" to edit the gradient table parameters, then click "Confirm" to save the changes, and then click "Save" to save the method changes. S4: The operation is divided into manual operation and automatic operation. The manual operation is as follows: click the "Home" tab in the navigation bar to switch to the operation interface, click the "Manual" button to enter the manual operation mode, click the "UV" button, and the "Light On", "Light Off" and "Zero" function buttons will appear. Click the "UV" button again to hide the function buttons. Click the value in the "Channel 1" or "Channel 2" indication area to pop up the wavelength modification window, modify the wavelength value, and click "Execute" to modify the current wavelength of the detector; click "Light On" and "Execute" to turn on the deuterium lamp of the detector, click "Light Off" and "Execute" to turn off the deuterium lamp of the detector, and click "Zero" and "Execute" to reset the current detector dual-channel absorption value to zero; Manual injection: Click the "Set flow rate" numerical input box to set the flow rate, click "Execute" to save the change, click the "Set injection time" numerical input box to set the injection time, click "Execute" to save the change, the body 1 is provided with an injection pump, place the reagent bottle on the clamping component 51, when injection is required, extend the injection tube 3 into the reagent bottle, click "RUN" to start the injection pump, and the injection can be carried out. Click "STOP" to stop the injection pump, and you can view the running data batch and save path; Manual elution: Click the "Set flow" numerical input box to set the flow, click "Execute" to save the changes, click the "Set elution time" numerical input box to set the elution time, click "Execute" to save the changes, the main body 1 is equipped with pump A and elution pump, click the "A pump ratio" numerical input box to set the A pump ratio, click "Execute" to save the changes, click "RUN" to run the elution pump, click "PAUSH" to pause the elution pump, click again to continue running the pump, click "STOP" to end the elution pump, view the running data batch and save path; S5: Automatic operation: Click the "Home" tab in the navigation bar to switch to the operation interface, click the "Auto" button to enter the automatic operation mode, edit the sequence, click "Sequence Settings", enter the sequence name, click the blank box, the method selection window pops up, select the batch number, click "Select" to confirm the selection, click "+" to add a row of methods, select a row of methods, right-click the mouse, a function list pops up, select Delete Row or Clear All Rows to clear the sequence method, after editing, click "Save" to save the sequence, or click "Load" to load the sequence into the system for running. When you need to call a saved or loaded sequence, click the arrow button to pop up the list of sequences that have been run, click "Select" of the selected sequence to copy the method list of the sequence; Click "RUN" to start the pump, click "PAUSH" to pause the pump, click again to continue the pump, click "STOP" to stop the pump, click "NEXT" to end the current method of the sequence and switch to the next method, click "Gradient Table" to pop up the gradient table of the current running method of the sequence, and view the running data batch and save path; S6: View spectrum: You can view the UV detector spectrum, run the detection spectrum, view the alarm list, and finally perform data analysis and data backup.

[0033] The operating principle of the present invention is: the operator opens the cover 21, moves the shielding component 53, and then places the reagent bottle between the two clamping plates 516 of each group of clamping components 51, so that the bottom of the reagent bottle abuts against the buffer pad 522, and then moves the shielding mechanism to shield the open reagent bottle, reduce the entry of dust and other impurities, so as not to affect the detection effect.

[0034] Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

Claims

1. A high performance liquid chromatograph, characterized in that: The invention comprises a main body (1), a placement box (2) fixed on the top of the main body (1), a sample injection tube (3), a lifting mechanism (4), and two sets of fixing mechanisms (5); the placement box (2) is hollow inside and has an open top; the sample injection tube (3) is a hose, one end of which extends into the main body (1) and the other end is located in the placement box (2); the lifting mechanism (4) is arranged on the bottom surface of the placement box (2); a mounting block (42) is fixed on the lifting mechanism (4); a mounting plate (43) is symmetrically fixed on the mounting block (42); and the two sets of fixing mechanisms (5) are symmetrically arranged on the mounting plate (43); Each set of the fixing mechanisms (5) comprises a plurality of clamping assemblies (51), a plurality of supporting assemblies (52), and a plurality of shielding assemblies (53), and the three correspond to each other. Each set of the clamping assemblies (51) comprises a mounting box (511) fixed on the top surface of the mounting plate (43), two vertical plates (512), two first baffles (513), a sliding plate (514) arranged along the length direction of the mounting box (511), two second baffles (515), two The clamping plate (516) is provided, the installation box (511) is hollow inside and is opened on one side away from the installation plate (43), the two vertical plates (512) are fixed vertically on the bottom surface of the installation box (511), and a gap is left between the two for the sliding plate (514) to pass through, the two first baffles (513) are fixed vertically on the bottom surface of the installation box (511) at one end away from the installation plate (43), and a gap is left between the two for the sliding plate (514) to pass through, the sliding One end of the plate (514) passes through the gap between the two vertical plates (512) and is fixed with a spring (517) between the side wall of the installation box (511) close to the installation plate (43). The other end passes through the gap between the two first baffles (513) and is rotatably connected to the first roller (5141). The two second baffles (515) are symmetrically arranged on the sliding plate (514) between the vertical plate (512) and the first baffle (513). The second baffles (515) One end is fixed on the sliding plate (514), and the other end is tilted away from the sliding plate (514). The bottom surface of the installation box (511) is fixed with rotating rods (5111) on both sides of the first baffle (513). The two clamping plates (516) are rotatably connected to the rotating rods (5111). One end of the clamping plate (516) close to the sliding plate (514) is rotatably connected to the second roller (518), and the other end is rotatably connected to the third roller (519).

2. The high performance liquid chromatograph according to claim 1, wherein: The lifting mechanism (4) comprises a cylinder (41), the bottom end of the cylinder (41) is fixed on the bottom surface of the placement box (2), the piston rod of the cylinder (41) faces upward, and the mounting block (42) is fixed on the top end of the piston rod of the cylinder (41).

3. The high performance liquid chromatograph according to claim 1, wherein: One end of the clamping plate (516) close to the sliding plate (514) is a straight plate, and the other end is a curved plate.

4. The high performance liquid chromatograph according to claim 1, wherein: The support assembly (52) includes a first fixing plate (521), which is arranged in an "L" shape. One end of the first fixing plate (521) is fixed on the mounting plate (43), and the other end extends below the clamping plate (516).

5. The high performance liquid chromatograph according to claim 4, wherein: A buffer pad (522) is fixedly provided on the first fixing plate (521).

6. The high performance liquid chromatograph according to claim 1, wherein: The shielding assembly (53) comprises a second fixed plate (531) vertically fixed on the installation box (511), a slider (532), a connecting plate (533), and a third baffle (534) located above the clamping plate (516); the second fixed plate (531) is provided with a sliding groove (5311) for the slider (532) to slide; the connecting plate (533) passes through the slider (532) and is rotatably connected thereto; one end of the connecting plate (533) is fixed to the third baffle (534), and the other end is fixed with a knob (535).

7. The high performance liquid chromatograph according to claim 1, wherein: The placement box (2) is hinged with a cover plate (21) at the opening, and a handle (22) is fixed on the top surface of the cover plate (21).

8. A method for controlling a high performance liquid chromatograph according to any one of claims 1 to 7, characterized in that: The steps include: S1: Start chromatography software; S2: set parameters; S3: Create instrument method and edit; S4: The operation is divided into manual operation and automatic operation, among which the manual operation is divided into manual injection and manual elution; S5: Automatic operation; S6: View the graph.

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