Feed additive for preventing sheep brucellosis and preparation method thereof

Abstract

The invention provides a feed additive for preventing sheep brucellosis and a preparation method thereof, and belongs to the technical field of animal feed additives. The feed additive for preventing sheep brucellosis is prepared from the following components in parts by weight: 25-35 parts of radix scutellariae, 18-24 parts of herba houttuyniae, 15-20 parts of herba andrographitis, 12-16 parts of herba portulacae, 10-15 parts of radix angelicae sinensis, 10-15 parts of colla corii asini, 5-10 parts of rhizoma atractylodis macrocephalae, 4-8 parts of coix seeds and 3-5 parts of radix glycyrrhizae. The components of the feed additive are used together, so that the feed additive can inhibit the growth and reproduction of brucella, regulate the gastrointestinal health of animals, improve the immunity and enhance the disease resistance of the animals, thereby effectively preventing and controlling sheep brucellosis. The feed additive disclosed by the invention is good in prevention and treatment effect, small in toxic and side effects and not easy to generate drug resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of animal feed additives, and in particular relates to a feed additive for preventing sheep brucellosis and a preparation method thereof. Background Art

[0002] Brucellosis (also known as brucellosis or "brucellosis") is a zoonotic disease caused by the bacterium Brucella (also known as Brucella) and is widespread worldwide. Brucellosis primarily spreads among livestock such as sheep, cattle, and pigs, causing economic losses to the livestock industry. Adult animals infected with the disease can suffer reproductive system damage, with males experiencing orchitis and epididymitis, and pregnant females experiencing premature birth and miscarriage.

[0003] Currently, antibiotics are the first choice for treating brucellosis, but long-term use of antibiotics brings many hazards, such as the increase of drug-resistant strains, endocrine dysfunction, excessive antibiotic residues and veterinary drug residues, etc., which seriously affect human health. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a feed additive for preventing sheep brucellosis and a preparation method thereof, so as to solve the problems of excessive antibiotic residues and veterinary drug residues generated by existing drug treatments.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a feed additive for preventing sheep brucellosis. The feed additive comprises the following components in parts by weight: 25-35 parts of scutellaria baicalensis, 18-24 parts of houttuynia cordata, 15-20 parts of andrographis paniculata, 12-16 parts of purslane, 10-15 parts of angelica sinensis, 10-15 parts of donkey-hide gelatin, 5-10 parts of atractylodes macrocephala, 4-8 parts of coix seed, and 3-5 parts of liquorice.

[0007] Preferably, the following components are included in parts by weight: 28-32 parts of Scutellaria baicalensis, 20-22 parts of Houttuynia cordata, 16-19 parts of Andrographis paniculata, 13-15 parts of Portulaca oleracea, 11-14 parts of Angelica sinensis, 11-14 parts of donkey-hide gelatin, 6-9 parts of Atractylodes macrocephala, 5-7 parts of Coix seed, and 4 parts of Licorice.

[0008] The present invention also provides a method for preparing the feed additive, comprising the following steps:

[0009] 1) Scutellaria baicalensis, donkey-hide gelatin, coix seed and water are mixed, decocted, and filtered to obtain filtrate A;

[0010] 2) Grinding Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis separately, mixing, and reflux extraction to obtain extract A;

[0011] 3) soaking the mixed angelica and atractylodes macrocephala in water, and extracting by steam distillation to obtain extract B;

[0012] 4) The filtrate A, the extract A and the extract B are mixed and spray-dried to obtain the feed additive.

[0013] Preferably, in step 1), the mass-to-volume ratio of the total mass of the scutellaria baicalensis, donkey-hide gelatin, and coix seed to water is 1 g:8-12 mL; the decoction time is 1-3 h; and the decoction is repeated 2-4 times.

[0014] Preferably, the solvent used in the reflux extraction in step 2) is an ethanol solution with a volume concentration of 70% to 95%; the mass-to-volume ratio of the total mass of Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Licorice to the ethanol solution is 1 g:5 to 10 mL.

[0015] Preferably, the reflux extraction in step 2) is performed 2 to 4 times; and the reflux extraction time is 2 to 4 hours per time.

[0016] Preferably, the conditions for the steam distillation extraction in step 3) are: the mass-to-volume ratio of the total mass of the angelica and atractylodes to water is 1 g:8-12 mL; the soaking time is 1-3 h; and the extraction time is 4-8 h.

[0017] Preferably, the spray drying temperature is 80-120° C., and the spray drying time is 2-4 hours.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention comprises scutellaria baicalensis, herba houttuyniae, herba andrographis paniculatae, herba purslane, herba angelicae sinensis, herba donkey-hide gelatin, herba atractylodes macrocephalae, herba coicis, and herba licorice, which synergistically enhance the antibacterial, heat-clearing and detoxifying, and spleen-strengthening effects. The herba licorice, in addition to containing multiple active ingredients with detoxifying properties, can also harmonize the medicinal properties of the overall formula and serve as an adjuvant. The present invention comprises various components used in combination to inhibit the growth and reproduction of Brucella, regulate the gastrointestinal health of animals, enhance immunity, strengthen the animals' disease resistance, and thereby effectively prevent and control brucellosis in sheep. The feed additive of the present invention not only has a good preventive and therapeutic effect, but also has minimal toxic and side effects and is less likely to develop drug resistance. DETAILED DESCRIPTION

[0020] The invention provides a feed additive for preventing sheep brucellosis. The feed additive comprises the following components in parts by weight: 25-35 parts of scutellaria baicalensis, 18-24 parts of houttuynia cordata, 15-20 parts of andrographis paniculata, 12-16 parts of purslane, 10-15 parts of angelica sinensis, 10-15 parts of donkey-hide gelatin, 5-10 parts of atractylodes macrocephala, 4-8 parts of coix seed, and 3-5 parts of liquorice.

[0021] In the present invention, the dosage of Scutellaria baicalensis is preferably 28 to 32 parts. Scutellaria baicalensis is bitter and cold in nature; it enters the lung, gallbladder, spleen, large intestine, and small intestine meridians; it clears heat and dampness, purges fire and detoxifies, stops bleeding, and stabilizes pregnancy. Scutellaria baicalensis contains a large number of flavonoids, glycosides, terpenes, trace elements, enzymes, sterols, organic acids, and other substances, and has anti-inflammatory, antipyretic, sedative, analgesic, and antibacterial effects, with a wide range of antibacterial properties.

[0022] In the present invention, the dosage of Houttuynia cordata is preferably 18 to 24 parts. Houttuynia cordata is pungent and cold; it enters the liver and lung meridians; it has multiple benefits, including clearing heat and detoxifying, promoting diuresis and relieving stranguria, eliminating carbuncle and pus, and providing antibacterial and anti-inflammatory effects, as well as enhancing immunity. Houttuynia cordata has inhibitory effects on various microorganisms, with significant inhibitory effects on hemolytic Streptococcus, influenza bacilli, and pneumococci. It also has effects on Escherichia coli and Salmonella typhi, demonstrating extremely strong antibacterial effects.

[0023] In the present invention, the dosage of Andrographis paniculata is preferably 16 to 19 parts. Andrographis paniculata is bitter and cold in nature; it enters the Heart, Lung, Large Intestine, and Bladder meridians; it has the effects of clearing heat and detoxifying, cooling blood and reducing swelling, drying dampness, and providing antibacterial and anti-inflammatory effects, as well as enhancing immunity. Andrographis paniculata has an inhibitory effect on various bacteria. The active ingredients in Andrographis paniculata can destroy bacterial cell walls, inhibiting bacterial growth and reproduction, thereby achieving an antibacterial effect.

[0024] In the present invention, the amount of purslane is preferably 13 to 15 parts. Purslane tastes sour and is cold in nature; it enters the liver meridian and the large intestine meridian; it clears away heat and detoxifies, disperses blood and reduces swelling. It is used for bloody stools and uterine bleeding and has a hemostatic effect. Purslane contains a variety of active ingredients, including flavonoids, alkaloids, organic acids, polysaccharides and proteins, etc., which have pharmacological activities such as antibacterial, anti-ulcer, anti-inflammatory and promoting wound healing. It is suitable for preventing and treating various animal diseases caused by these bacteria, such as mastitis, dermatitis, arthritis in dairy sheep and diarrhea in piglets.

[0025] In the present invention, the dosage of angelica is preferably 11 to 14 parts. Angelica sinensis has a sweet, pungent, and bitter flavor and is warm in nature. It enters the liver, heart, and spleen meridians and has the effects of nourishing blood and activating blood circulation, regulating menstruation and relieving pain, moistening the intestines and promoting bowel movements, dispelling wind and dampness, and enhancing immunity. It is primarily used to treat reproductive system disorders such as postpartum hemorrhage, retained placenta, and lochia. It also has antibacterial effects against dysentery, typhoid fever, paratyphoid fever, Escherichia coli, diphtheria, Vibrio cholerae, and α- and β-hemolytic streptococci.

[0026] In the present invention, the dosage of the donkey-hide gelatin is preferably 11 to 14 parts; donkey-hide gelatin tastes sweet and is neutral in nature; it enters the lung meridian, liver meridian, and kidney meridian; it nourishes yin and moistens dryness, replenishes blood, stops bleeding, and calms the fetus; it is used for various bleeding syndromes, yin deficiency syndromes, and dryness syndromes, hematemesis and hematuria, bloody stools and metrorrhagia, pregnancy and fetal bleeding, yin damage caused by febrile diseases, restlessness and insomnia, and cough and hemoptysis caused by fatigue; the nutrients in donkey-hide gelatin can enhance the immunity of animals, improve their resistance, and prevent the occurrence of diseases.

[0027] In the present invention, the dosage of Atractylodes macrocephala is preferably 6 to 9 parts. Atractylodes macrocephala has a bitter and sweet taste and is warm in nature. It enters the spleen and stomach meridians and has the effects and efficacy of strengthening the spleen and replenishing qi, drying dampness and promoting diuresis, strengthening the exterior and stopping sweating, stabilizing pregnancy, and having anti-inflammatory and antibacterial effects. It can regulate spleen and stomach function, enhance yang energy in the body, and reduce dampness retention, and is beneficial for the spleen and kidney yang deficiency type of orchitis.

[0028] In the present invention, the dosage of coix seed is preferably 5 to 7 parts; coix seed tastes sweet, light, and slightly cold; enters the spleen meridian, lung meridian, and kidney meridian; strengthens the spleen, nourishes the lungs, clears away heat, and promotes dampness; and can relieve edema caused by orchitis and epididymitis.

[0029] In the present invention, the dosage of licorice is preferably 4 parts. Licorice is sweet and neutral in nature; it enters the spleen, stomach, and lung meridians; and has the functions of replenishing qi and tonifying the middle, removing phlegm and relieving cough, detoxifying, relieving pain, and alleviating the effects of the drug. Licorice contains rich nutrients, such as glycyrrhizic acid and glycyrrhizin, which can promote the growth and development of animals and enhance their immunity.

[0030] The present invention also provides a method for preparing the feed additive, comprising the following steps:

[0031] 1) Scutellaria baicalensis, donkey-hide gelatin, coix seed and water are mixed, decocted, and filtered to obtain filtrate A;

[0032] 2) Grinding Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis separately, mixing, and reflux extraction to obtain extract A;

[0033] 3) soaking the mixed angelica and atractylodes macrocephala in water, and extracting by steam distillation to obtain extract B;

[0034] 4) The filtrate A, the extract A and the extract B are mixed and spray-dried to obtain the feed additive.

[0035] In the present invention, scutellaria baicalensis, donkey-hide gelatin, coix seed, and water are mixed, decocted, and filtered to obtain filtrate A. The mass-to-volume ratio of the total mass of scutellaria baicalensis, donkey-hide gelatin, and coix seed to water is preferably 1 g:8-12 mL, more preferably 1 g:9-11 mL; the decocting time is preferably 1-3 h, more preferably 1.5-2.5 h; and the number of decocting times is preferably 2-4 times, more preferably 3 times.

[0036] In the present invention, houttuynia cordata, andrographis paniculata, purslane, and licorice are pulverized separately and then mixed, and reflux extracted to obtain extract A. The solvent used in the reflux extraction is preferably an ethanol solution with a volume concentration of 70% to 95%, and the volume concentration of the ethanol solution is more preferably 75 to 90%; the mass-to-volume ratio of the total mass of houttuynia cordata, andrographis paniculata, purslane, and licorice to the ethanol solution is preferably 1 g:5 to 10 mL, and more preferably 1 g:6 to 9 mL; the number of reflux extractions is preferably 2 to 4 times, and more preferably 3 times; and the time of the reflux extraction is preferably 2 to 4 hours per time, and more preferably 2.5 to 3.5 hours per time.

[0037] In the present invention, a mixture of angelica and atractylodes is immersed in water and extracted by steam distillation to obtain an extract B. The steam distillation extraction is specifically as follows: the mixture of angelica and atractylodes is immersed in water and placed in a round-bottom flask, water is added from the upper end of the condenser until it fills the scale part of the volatile oil measuring instrument and overflows into the flask, the flask is placed in an electric heating mantle and heated to boiling, and the extraction is maintained at a slight boiling point, then the heating is stopped, the mixture is allowed to stand for a while, the piston at the lower end of the measuring instrument is opened, the water is slowly released, and the extract B is collected. The conditions for the steam distillation extraction are: the mass-to-volume ratio of the total mass of the angelica and atractylodes to water is preferably 1g:8-12mL, more preferably 9-11mL; the soaking time is preferably 1-3h, more preferably 1.5-2.5h; the extraction time is preferably 4-8h, more preferably 5-7h.

[0038] In the present invention, filtrate A, extract A, and extract B are mixed and spray-dried to obtain the feed additive. The spray-drying temperature is preferably 80-120° C., more preferably 90-110° C.; the spray-drying time is preferably 2-4 hours, more preferably 2.5-3.5 hours.

[0039] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0040] Example 1

[0041] A feed additive for preventing sheep brucellosis consists of the following components in parts by weight: 30 parts of scutellaria baicalensis, 20 parts of houttuynia cordata, 18 parts of andrographis paniculata, 14 parts of purslane, 12 parts of angelica sinensis, 12 parts of donkey-hide gelatin, 8 parts of atractylodes macrocephala, 6 parts of coix seed, and 4 parts of liquorice.

[0042] The preparation method of the feed additive is:

[0043] Fresh, intact, insect-free, mildew-free, and unmixed scutellaria baicalensis, donkey-hide gelatin, and coix seed were mixed with 10 times the total mass of water and decocted three times, each time for 2 hours. After each decoction, the residue was separated and the filtrate was collected. The filtrates were then combined to obtain filtrate A.

[0044] Fresh, intact, insect-free, mildew-free, and unmixed Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis were selected and ground separately, then passed through a 70-mesh sieve. The sieve residues were collected and mixed, and 85% ethanol solution was added at a mass-to-volume ratio of 1 g:8 mL of the total mass of Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis to the ethanol solution, and refluxed and extracted three times for 3 hours each time to obtain Extract A.

[0045] Select fresh, intact, insect-free, mildew-free, and uncontaminated Angelica sinensis and Atractylodes macrocephala, crush and mix them, place in a round-bottom flask, and add water and several glass beads in a mass-to-volume ratio of 1 g of Angelica sinensis and Atractylodes macrocephala to water (10 mL). Soak for 2 hours. Add water from the top of the condenser tube until the scale on the volatile oil analyzer is filled and overflows into the flask. Heat the flask in an electric heating mantle to boiling and maintain a slight boiling for 6 hours. Remove the heat, let it stand for a while, open the stopcock at the bottom of the analyzer, slowly drain the water, and collect the extract, Solution B.

[0046] The filtrate A, the extract A and the extract B were mixed, and spray-dried at a temperature of 10° C. for 3 hours to obtain the feed additive.

[0047] Example 2

[0048] A feed additive for preventing sheep brucellosis consists of the following components in parts by weight: 25 parts of scutellaria baicalensis, 18 parts of houttuynia cordata, 15 parts of andrographis paniculata, 12 parts of purslane, 10 parts of angelica sinensis, 10 parts of donkey-hide gelatin, 5 parts of atractylodes macrocephala, 4 parts of coix seed, and 3 parts of liquorice.

[0049] The preparation method of the feed additive is:

[0050] Fresh, intact, insect-free, mildew-free, and unmixed scutellaria baicalensis, donkey-hide gelatin, and coix seed were mixed with 8 times their total mass of water and decocted twice, each time for 1 hour. After each decoction, the residue was separated and the filtrate was collected. The filtrates were then combined to obtain filtrate A.

[0051] Fresh, intact, insect-free, mildew-free, and unmixed Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis were selected and ground separately, then passed through a 60-mesh sieve. The sieve residues were collected and mixed, and 70% ethanol solution was added at a mass-to-volume ratio of 1 g:5 mL of the total mass of Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis to the ethanol solution, and refluxed and extracted twice for 2 h each time to obtain Extract A.

[0052] Select fresh, intact, insect-free, mildew-free, and uncontaminated Angelica sinensis and Atractylodes macrocephala, crush and mix them, place in a round-bottom flask, and add water and several glass beads in a mass-to-volume ratio of 1g:8mL for the total weight of Angelica sinensis and Atractylodes macrocephala. Soak for 1 hour. Add water from the top of the condenser tube until the scale on the volatile oil analyzer is filled and overflows into the flask. Heat the flask in an electric heating mantle to boiling and maintain a slight boiling for 4 hours. Remove the heat, let it stand for a while, open the stopcock at the bottom of the analyzer, slowly drain the water, and collect the extract, Solution B.

[0053] The filtrate A, the extract A and the extract B were mixed, and spray-dried at 80° C. for 2 h to obtain the feed additive.

[0054] Example 3

[0055] A feed additive for preventing sheep brucellosis consists of the following components in parts by weight: 35 parts of scutellaria baicalensis, 24 parts of houttuynia cordata, 20 parts of andrographis paniculata, 16 parts of purslane, 15 parts of angelica sinensis, 15 parts of donkey-hide gelatin, 10 parts of atractylodes macrocephala, 8 parts of coix seed, and 5 parts of liquorice.

[0056] The preparation method of the feed additive is:

[0057] Fresh, intact, insect-free, mildew-free, and unmixed scutellaria baicalensis, donkey-hide gelatin, and coix seed were mixed with 12 times their total mass of water and decocted four times, each time for 3 hours. After each decoction, the residue was separated and the filtrate was collected. The filtrates were then combined to obtain filtrate A.

[0058] Fresh, intact, insect-free, mildew-free, and unmixed Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis were selected and ground separately, then passed through an 80-mesh sieve. The sieve residues were collected and mixed, and 95% ethanol solution was added at a mass-to-volume ratio of 1 g:10 mL of the total mass of Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis to the ethanol solution, and refluxed and extracted four times for 4 hours each time to obtain Extract A.

[0059] Select fresh, intact, insect-free, mildew-free, and uncontaminated Angelica sinensis and Atractylodes macrocephala, crush and mix them, place in a round-bottom flask, and add water and several glass beads in a mass-to-volume ratio of 1g:12mL for the total weight of Angelica sinensis and Atractylodes macrocephala. Soak for 3 hours. Add water from the top of the condenser tube until the scale on the volatile oil analyzer is filled and overflows into the flask. Heat the flask in an electric heating mantle to boiling and maintain a slight boiling for 8 hours. Remove the heat, let it stand for a while, open the stopcock at the bottom of the analyzer, slowly drain the water, and collect the extract, Solution B.

[0060] The filtrate A, the extract A and the extract B were mixed, and spray-dried at a temperature of 120° C. for 4 hours to obtain the feed additive.

[0061] Comparative Example 1

[0062] The difference from Example 1 is that Scutellaria baicalensis is not added.

[0063] Comparative Example 2

[0064] The difference from Example 1 is that Houttuynia cordata is not added.

[0065] Comparative Example 3

[0066] The difference from Example 1 is that the amount of Scutellaria baicalensis is 20 parts.

[0067] Comparative Example 4

[0068] The difference from Example 1 is that the amount of Scutellaria baicalensis is 40 parts.

[0069] Comparative Example 5

[0070] The difference from Example 1 is that water is added according to the mass volume ratio of the total mass of Angelica sinensis and Atractylodes macrocephala to water of 1 g:10 mL, and the mixture is decocted three times, each time for 2 hours. After each decoction, the mixture is filtered to separate the residue and the filtrate is collected.

[0071] Experimental Example 1

[0072] Nine goat farms of the same breeding scale were randomly selected, the sites were disinfected, and after one week of natural aging, 400 goats were newly introduced to each site for conventional breeding. The goats in the nine sites were all raised by the same person. The feed additives prepared in Examples 1 to 3 and Comparative Examples 1 to 5 were added to the goats in the eight farms at a ratio of 1% of the daily diet. The ninth farm served as a control group and no feed additive was added.

[0073] The serological test method used was the tube agglutination test (SAT) in accordance with GB / T 18646-2018.

[0074] Experimental results: as shown in Table 1.

[0075] Table 1 Serological test results of different treatment groups

[0076]

[0077]

[0078] As shown in Table 1, the positive serum antibody rate of goats fed with the feed additive groups prepared in Examples 1 to 3 of the present invention was much lower than that in Comparative Examples 1 to 5 and the control group, indicating that the feed additive of the present invention has a significant preventive effect on brucellosis.

[0079] Experimental Example 2

[0080] At a goat breeding base, 200 goats were selected and tested using a goat brucellosis ELISA kit (purchased from Jiangsu Boshen Biotechnology Co., Ltd.). Among them, 148 goats were positive for brucellosis and 52 were negative. They were fed ordinary, commercially available compound feed and added with 1% of the feed additive prepared in Example 1. They were fed directly in the morning and evening every day for a test period of 60 days. After 60 days, the development of goat brucellosis symptoms during the test period was tested. The specific results are shown in Table 2 below.

[0081] Table 2 Development of Brucellosis Symptoms in Goats in Different Treatment Groups

[0082]

[0083] As shown in Table 2, after feeding the feed additive prepared in Example 1, the number of goats positive for brucellosis decreased from 74% to 48.5%, a decrease of 25.5%, indicating that the feed additive of the present invention has a significant control effect on goat brucellosis.

[0084] It can be seen from the above embodiments and experimental examples that the feed additive prepared by the present invention has obvious prevention and control effects on brucellosis in sheep.

[0085] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A feed additive for preventing sheep brucellosis, characterized in that: The invention comprises the following components in parts by weight: 25-35 parts of scutellaria baicalensis, 18-24 parts of houttuynia cordata, 15-20 parts of andrographis paniculata, 12-16 parts of purslane, 10-15 parts of angelica sinensis, 10-15 parts of donkey-hide gelatin, 5-10 parts of atractylodes macrocephala, 4-8 parts of coix seed and 3-5 parts of liquorice.

2. The feed additive according to claim 1, characterized in that The invention comprises the following components in parts by weight: 28-32 parts of scutellaria baicalensis, 20-22 parts of houttuynia cordata, 16-19 parts of andrographis paniculata, 13-15 parts of purslane, 11-14 parts of angelica sinensis, 11-14 parts of donkey-hide gelatin, 6-9 parts of atractylodes macrocephala, 5-7 parts of coix seed and 4 parts of liquorice.

3. The method for preparing the feed additive according to claim 1 or 2, characterized in that: The following steps are involved: 1) Scutellaria baicalensis, donkey-hide gelatin, coix seed and water are mixed, decocted, and filtered to obtain filtrate A; 2) Grinding Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Glycyrrhiza uralensis separately, mixing, and reflux extraction to obtain extract A; 3) soaking the mixed angelica and atractylodes macrocephala in water, and extracting by steam distillation to obtain extract B; 4) The filtrate A, the extract A and the extract B are mixed and spray-dried to obtain the feed additive.

4. The preparation method according to claim 3, characterized in that Step 1) The mass volume ratio of the total mass of the scutellaria baicalensis, donkey-hide gelatin, and coix seed to water is 1g:8-12mL; the decocting time is 1-3h; and the decocting times are 2-4 times.

5. The preparation method according to claim 3, characterized in that Step 2) The solvent used in the reflux extraction is an ethanol solution with a volume concentration of 70% to 95%; the mass volume ratio of the total mass of the Houttuynia cordata, Andrographis paniculata, Portulaca oleracea, and Licorice to the ethanol solution is 1g:5 to 10mL.

6. The preparation method according to claim 3, characterized in that Step 2) The reflux extraction is performed 2 to 4 times; and the reflux extraction time is 2 to 4 hours per time.

7. The preparation method according to claim 3, characterized in that Step 3) The steam distillation extraction conditions are as follows: the mass volume ratio of the total mass of the angelica and white atractylodes to water is 1g:8-12mL; the soaking time is 1-3h; and the extraction time is 4-8h.

8. The preparation method according to claim 3, characterized in that The spray drying temperature is 80-120° C., and the spray drying time is 2-4 hours.

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