Krill oil microcapsule powder with high embedding rate as well as preparation method and application of krill oil microcapsule powder

Abstract

The invention relates to krill oil micro-capsule powder with a high embedding rate as well as a preparation method and application of the krill oil micro-capsule powder. Specifically, the invention discloses krill oil micro-capsule powder which comprises (a) an active ingredient which comprises krill oil; (b) sodium caseinate; and (c) a filler, wherein the filler comprises maltodextrin; moreover, the embedding rate of the krill oil microcapsule powder is greater than or equal to 80%. The krill oil microcapsule powder disclosed by the invention has the characteristics of high embedding rate, high oil loading capacity, good product stability, high bioavailability and uniform particle size, and can be applied to baked foods, solid beverages, health foods, dairy products, capsule products and special medical foods.

Description

Technical Field

[0001] The present invention relates to the field of food processing, and in particular to krill oil microcapsule powder with high embedding rate, a preparation method and application thereof. Background Art

[0002] Antarctic krill oil is rich in phospholipids and contains a high concentration of the ω-3 polyunsaturated fatty acids EPA and DHA, making it an excellent health food ingredient with promising development and utilization prospects. Antarctic krill is rich in natural nutrients and has a wide range of benefits for human health. Research has shown that phospholipid-rich Antarctic krill oil can improve memory, lower blood sugar and lipids, and prevent Alzheimer's disease, cardiovascular and cerebrovascular diseases, and atherosclerosis. Krill oil's marine phospholipid-based ω-3 fatty acids have a 99% absorption rate and can regulate blood lipids, blood pressure, weight, and blood sugar, improve cognition, and have anti-inflammatory properties. Choline can nourish the nervous system and enhance memory, while astaxanthin, a natural antioxidant, can protect the body against oxidative aging. Krill oil is rich in n-3 PUFAs (polyunsaturated fatty acids), phospholipids (PL), astaxanthin, vitamins, flavonoids, and minerals. The phospholipid forms of DHA and EPA in krill oil are more easily absorbed and utilized by the body than their triglyceride counterparts in fish oil, which has important implications for the prevention and treatment of cardiovascular disease. Krill oil also has potential anti-inflammatory and anti-tumor properties, improving insulin sensitivity and secretion to help manage diabetes. With rising global consumer health awareness and increasing market acceptance of krill oil, the Antarctic krill industry is poised to continue expanding, necessitating the high-value application of Antarctic krill resources and product upgrades.

[0003] Krill oil has strong antioxidant activity, but it also suffers from poor water solubility, a strong odor, and susceptibility to oxidative degradation. Domestically, the country's lack of independent development and innovation capabilities in DHA / EPA raw materials and encapsulation makes it susceptible to technical barriers, resulting in high product costs, poor stability, and a strong odor, limiting its application in the food industry.

[0004] Therefore, there is an urgent need in the art to develop a krill oil microcapsule powder with high encapsulation efficiency, high oil loading, good product stability and high bioavailability. Summary of the Invention

[0005] The purpose of the present invention is to provide a krill oil microcapsule powder with high embedding rate, high oil loading, high bioavailability and good product stability.

[0006] The first aspect of the present invention provides a krill oil microcapsule powder, comprising:

[0007] (a) an active ingredient, the active ingredient comprising krill oil;

[0008] (b) sodium caseinate; and

[0009] (c) a bulking agent, the bulking agent comprising maltodextrin;

[0010] Furthermore, the embedding rate of the krill oil microcapsule powder is ≥80%.

[0011] In another preferred embodiment, the krill oil is Antarctic krill oil.

[0012] In another preferred embodiment, the krill oil microcapsule powder is composed of a core material and a wall material, wherein the core material includes the active ingredient; and the wall material includes sodium caseinate and a filler.

[0013] In another preferred embodiment, the filler is selected from maltodextrin, resistant dextrin or a combination thereof.

[0014] In another preferred embodiment, the krill oil microcapsule powder has the following components in parts by weight:

[0015] 15-30 parts by weight of active ingredient

[0016] Filler 65-80 parts by weight

[0017] 3 to 15 parts by weight of sodium caseinate

[0018] In another preferred embodiment, the krill oil microcapsule powder further comprises a thickener and / or an emulsifier.

[0019] In another preferred embodiment, the thickener is selected from the group consisting of sodium starch octenylsuccinate, gum arabic, gelatin or a combination thereof.

[0020] In another preferred embodiment, the emulsifier includes mono- and diglyceride fatty acids.

[0021] In another preferred embodiment, the krill oil microcapsule powder contains the following components:

[0022]

[0023] In another preferred embodiment, the krill oil microcapsule powder comprises:

[0024] Active ingredients, sodium caseinate, maltodextrin, sodium starch octenylsuccinate and mono- and diglycerides of fatty acids.

[0025] In another preferred embodiment, the krill oil microcapsule powder has the following components in parts by weight:

[0026]

[0027] In another preferred embodiment, the krill oil microcapsule powder contains the following components:

[0028]

[0029]

[0030] In another preferred embodiment, the average particle size of the krill oil microcapsule powder is 100-500 nm, preferably 150-400 nm, and more preferably 200-300 nm.

[0031] In another preferred embodiment, the oil loading amount of the krill oil microcapsule powder is ≥15%, preferably ≥18%, and more preferably ≥20%.

[0032] In another preferred embodiment, the encapsulation rate of the krill oil microcapsule powder is ≥85%, preferably ≥90%, and more preferably ≥91%.

[0033] In another preferred embodiment, the krill oil microcapsule powder is acid- and alkali-stable.

[0034] In another preferred embodiment, the acid-base stability refers to: within the pH range of 2 to 9, a relative change in the particle size of the krill oil microcapsule powder is from -50% to 100%, preferably from -25% to 50%; and / or a relative change in the potential of the krill oil microcapsule powder is from -70% to 20%.

[0035] In another preferred embodiment, the acid-base stability refers to: within the pH range of 2 to 9, the particle size of the krill oil microcapsule powder varies in the range of -20.3 nm to 103.7 nm; and / or the potential of the krill oil microcapsule powder varies in the range of -19 mV to 4.16 mV.

[0036] In another preferred embodiment, the krill oil microcapsule powder is thermally stable.

[0037] In another preferred embodiment, the thermal stability refers to: within the temperature range of 25-100° C., a relative change in the particle size of the krill oil microcapsule powder is -50%-60%, preferably -30%-40%; and / or a relative change in the potential of the krill oil microcapsule powder is 20%-80%, preferably 30%-60%.

[0038] In another preferred embodiment, the thermal stability means that within the temperature range of 25-100° C., the particle size of the krill oil microcapsule powder varies in the range of 11.2-30.5 nm; and the potential of the krill oil microcapsule powder varies in the range of 13.6-9.2 mV.

[0039] The krill oil microcapsule powder is stable at pH = 2 to 9, preferably at pH = 3 to 8, and more preferably at pH = 4 to 8.

[0040] In another preferred embodiment, the krill oil microcapsule powder is stable at 0-120°C, preferably 20-110°C, and more preferably 25-100°C.

[0041] In another preferred embodiment, the potential of the krill oil microcapsule powder is -47 to -35 mV, preferably -45 to -40 mV, and more preferably -45 to -42 mV.

[0042] In another preferred embodiment, the krill oil microcapsule powder is placed at room temperature for 6 months without any oil floating, precipitation or bottle sticking.

[0043] In another preferred embodiment, the DHA concentration P1 in the blood of a subject administered with the krill oil microcapsule powder is compared with the DHA concentration P0 in the blood of a subject administered with krill oil, and the ratio (P1 / P0) is ≥1.0, preferably ≥1.2, and more preferably ≥1.4.

[0044] In another preferred embodiment, the EPA concentration Z1 in the blood of a subject who has been administered the krill oil microcapsule powder is compared to the EPA concentration Z0 in the blood of a subject who has been administered krill oil, and the ratio (Z1 / Z0) between the two is ≥1.0, preferably ≥1.2, and more preferably ≥1.4.

[0045] In another preferred embodiment, the subject is a mammal, preferably a human or a non-human mammal (such as a rodent).

[0046] In another preferred embodiment, the krill oil microcapsule powder does not contain or substantially does not contain other oily components (ie, other oily components ≤ 1 wt %, preferably ≤ 0.5 wt %, based on the total weight of the krill oil microcapsule powder).

[0047] The second aspect of the present invention provides a method for preparing the krill oil microcapsule powder according to the first aspect of the present invention, the method comprising the steps of:

[0048] (s1) providing krill oil, sodium caseinate and fillers;

[0049] (s2) krill oil, sodium caseinate and maltodextrin are mixed, sheared, homogenized and dried to obtain krill oil microcapsule powder.

[0050] In another preferred embodiment, the filler includes maltodextrin, resistant dextrin or a combination thereof.

[0051] In another preferred embodiment, a thickener and / or an emulsifier is further provided in step (s1).

[0052] In another preferred embodiment, the method comprises the steps of:

[0053] (s1) providing a wall material and a core material, wherein the wall material comprises sodium caseinate, a filler, and a thickener; and the core material comprises krill oil and an emulsifier;

[0054] (s2) mixing, shearing, homogenizing, and drying the wall material and the core material to obtain krill oil microcapsule powder.

[0055] In another preferred embodiment, the filler is selected from the following group: maltodextrin or resistant dextrin.

[0056] In another preferred embodiment, the thickener is selected from the following group: sodium starch octenylsuccinate, gum arabic or gelatin.

[0057] In another preferred example, the weight proportions of sodium caseinate, filler and thickener in the wall material are 3-15, 65-80 and 1-5 respectively, preferably 3-10, 68-78 and 1-4, more preferably 3-8, 70-75 and 1-3, for example 5, 73 and 1.

[0058] In another preferred embodiment, the emulsifier includes mono- and diglyceride fatty acids.

[0059] In another preferred embodiment, the method comprises:

[0060] (s1) providing a wall material and a core material, wherein the first composition comprises sodium caseinate, maltodextrin, and sodium starch octenylsuccinate; and the second composition comprises krill oil and an emulsifier;

[0061] (s2) mixing, shearing, homogenizing, and drying the wall material and the core material to obtain krill oil microcapsule powder.

[0062] In another preferred embodiment, in step (s2), the mixing and shearing are performed simultaneously.

[0063] In another preferred example, the weight proportions of sodium caseinate, maltodextrin and sodium starch octenylsuccinate in the wall material are 3-15, 65-80 and 1-5 respectively, preferably 3-10, 68-78 and 1-4, more preferably 3-8, 70-75 and 1-3, for example 5, 73 and 1.

[0064] In another preferred embodiment, the weight proportions of krill oil and emulsifier in the core material are 15-30, 0.1-3.0, preferably 18-30, 0.5-1.5, more preferably 20-25, 0.7-1.5, for example 20 and 1 respectively.

[0065] In another preferred embodiment, the shearing is performed for 10 to 20 minutes, preferably 12 to 18 minutes, more preferably 13 to 17 minutes, for example 15 minutes.

[0066] In another preferred embodiment, the shearing is carried out at 8500-12000 r / min, preferably 9000-11000 r / min, more preferably 9500-11500 r / min, for example 10000 r / min.

[0067] In another preferred embodiment, in step (s2), the homogenization is performed at 30-40 MPa, preferably 32-38 MPa, and more preferably 34-36 MPa.

[0068] In another preferred embodiment, in step (s2), homogenization is performed twice, the first homogenization is performed at 60-80 Bar, and the second homogenization is performed at 330-370 Bar.

[0069] In another preferred embodiment, in step (s2), the drying is spray drying.

[0070] In another preferred embodiment, in step (s2), the drying injection temperature is 160-200°C, preferably 170-190°C, more preferably 175-185°C, for example 180°C.

[0071] In another preferred embodiment, in step (s2), the drying flow rate is 15-25 mL / min, preferably 17-23 mL / min, more preferably 18-21 mL / min, for example 20 mL / min.

[0072] In another preferred example, the preparation method of the wall material includes the following steps: taking 35 to 45 times the amount of sodium caseinate in water, heating the mixture to 60 to 80°C in a water bath, adding sodium caseinate, maltodextrin and / or sodium starch octenylsuccinate while stirring, mixing, and cooling the mixture to 45 to 55°C to obtain the wall material.

[0073] In another preferred embodiment, the method for preparing the core material comprises the following steps: mixing krill oil with mono- and diglyceride fatty acids to obtain the core material.

[0074] In another preferred embodiment, the wall material is prepared at 60-80°C, preferably 65-75°C, more preferably 68-72°C, for example 70°C.

[0075] In another preferred embodiment, the core material is prepared at 40-60°C, preferably 45-55°C, more preferably 48-52°C, for example 50°C.

[0076] In a third aspect, the present invention provides a use of the krill oil microcapsule powder according to the first aspect of the present invention for preparing food additives, baked goods, solid beverages, health foods, dairy products, capsule products, or special medical foods.

[0077] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features described in detail below (such as in the embodiments) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be listed here one by one. BRIEF DESCRIPTION OF THE DRAWINGS

[0078] Figure 1 The technical route for preparing krill oil microcapsule powder is shown.

[0079] Figure 2 Shows the changes in EPA entering the blood of mice in the animal blood pharmacokinetics test.

[0080] Figure 3 Shows the changes in DHA entering the blood of mice in the animal blood pharmacokinetics test. DETAILED DESCRIPTION

[0081] After extensive and in-depth research, numerous experiments, and screening, the present inventors unexpectedly discovered a krill oil microcapsule powder. This krill oil microcapsule powder exhibits high encapsulation efficiency, high oil loading, excellent product stability, high bioavailability, and uniform particle size. Furthermore, it exhibits excellent resolubility and lacks an odor. Compared to krill oil of equal concentration, its blood absorption rate is significantly higher than that of krill oil. This is the basis for the present invention.

[0082] the term

[0083] In order to make the present invention easier to understand, certain technical and scientific terms are specifically defined below. Unless otherwise clearly defined in this article, all other technical and scientific terms used herein have the meanings generally understood by those of ordinary skill in the art to which the present invention belongs. Before describing the present invention, it should be understood that the present invention is not limited to the specific methods and experimental conditions, because such methods and conditions can change. It should also be understood that the terms used herein are intended only to describe specific embodiments and are not intended to be restrictive, and the scope of the present invention will only be limited by the appended claims.

[0084] As used herein, the term “comprise” or variations thereof such as “include” or “comprising”, etc., is understood to include the stated elements or components but does not exclude other elements or components.

[0085] In the present invention, the term "active ingredient" refers to krill oil.

[0086] Microencapsulation technology

[0087] Microencapsulation, also known as microencapsulation, is a technique for encapsulating trace substances in polymer films. It is a micro-packaging technology for storing solids, liquids, and gases. Specifically, it involves completely encapsulating a target substance (core or inner phase) with a continuous film (wall or outer phase) of various natural or synthetic polymer compounds, without compromising the target's original chemical properties. The target's functions are then gradually re-emerged through certain external stimuli or sustained-release effects, or the core material is protected by the shielding effect of the capsule wall.

[0088] Krill Oil Microcapsule Powder

[0089] "Krill oil microcapsule powder," "compound of the present invention," "krill oil microcapsules of the present invention," "microcapsule powder of the present invention," and "microcapsule powder of the present invention" are used interchangeably to refer to krill oil microcapsule powder prepared by the method of the present invention, which exhibits high encapsulation efficiency, high oil loading, good product stability, and high bioavailability. The krill oil microcapsule powder comprises a core material comprising krill oil and a wall material comprising sodium caseinate, maltodextrin, and sodium starch octenylsuccinate.

[0090] The krill oil microcapsule powder of the present invention has the following components and weight proportions of each component:

[0091]

[0092] Wherein, the active ingredient is krill oil.

[0093] The added filler can be one or more ingredients that increase the weight and volume of the tablet. In the present invention, the filler is selected from maltodextrin or resistant dextrin. The filler accounts for 65-80% of the total amount of the krill oil microcapsule powder, preferably 68-78%, and more preferably 70-75%. In another preferred embodiment, the filler is a mixture of maltodextrin and resistant dextrin.

[0094] In the present invention, the thickener is selected from sodium starch octenylsuccinate, gum arabic, or gelatin. The thickener accounts for 1-5%, preferably 1-4%, and more preferably 1-3%, of the total amount of the krill oil microcapsule powder. In another preferred embodiment, the thickener is sodium starch octenylsuccinate, gum arabic, gelatin, or a mixture thereof.

[0095] In the present invention, the emulsifier includes mono- and diglycerol fatty acids, and accounts for 0.1 to 3.0% of the total amount of the krill oil microcapsule powder, preferably 0.5 to 1.5%, and more preferably 0.7 to 1.5%.

[0096] In the present invention, the krill oil microcapsule powder has an encapsulation efficiency of ≥80%, preferably ≥85%, more preferably ≥90%, for example, ≥91%, ≥92%, ≥93%, ≥94%, ≥95%, ≥96%, or ≥97%. The krill oil microcapsule powder has an oil loading capacity of ≥18%, preferably ≥20%, more preferably ≥25%, for example, ≥26%, ≥27%, ≥28%, ≥29%, or ≥30%.

[0097] In another preferred embodiment, the krill oil microcapsule powder is acid- and alkali-stable.

[0098] In another preferred embodiment, the acid-base stability refers to: within the pH range of 2 to 9, a relative change in the particle size of the krill oil microcapsule powder is from -50% to 100%, preferably from -25% to 50%; and / or a relative change in the potential of the krill oil microcapsule powder is from -70% to 20%.

[0099] In another preferred embodiment, the acid-base stability refers to: within the pH range of 2 to 9, the particle size of the krill oil microcapsule powder varies in the range of -20.3 nm to 103.7 nm; and / or the potential of the krill oil microcapsule powder varies in the range of -19 mV to 4.16 mV.

[0100] In another preferred embodiment, the krill oil microcapsule powder is thermally stable.

[0101] In another preferred embodiment, the thermal stability refers to: within the temperature range of 25-100° C., a relative change in the particle size of the krill oil microcapsule powder is -50%-60%, preferably -30%-40%; and / or a relative change in the potential of the krill oil microcapsule powder is 20%-80%, preferably 30%-60%.

[0102] In another preferred embodiment, the thermal stability means that within the temperature range of 25-100° C., the particle size of the krill oil microcapsule powder varies in the range of 11.2-30.5 nm; and the potential of the krill oil microcapsule powder varies in the range of 13.6-9.2 mV.

[0103] The krill oil microcapsule powder of the present invention has a high encapsulation rate, indicating that the krill oil is completely encapsulated by the wall material. Therefore, in actual products, the krill oil is not easily released, thereby achieving a sustained release effect, effectively improving taste, masking odors, and reducing the loss of active ingredients.

[0104] The krill oil microcapsule powder of the present invention uses a hydrophilic material as the wall material, which is hydrophilic, more soluble in water, and more easily absorbed. However, due to its poor solubility in water, krill oil has limited applications. The krill oil microcapsule powder produced using the present method can be applied to aqueous systems, expanding its scope of application.

[0105] The krill oil microcapsule powder of the present invention can be used as a food additive and added to liquid beverages, solid beverages, milk and dairy products, emulsions, health foods and other foods.

[0106] The features described above, or in the embodiments, may be combined in any combination. All features disclosed in this specification may be used in any combination, and each feature disclosed in this specification may be replaced by any alternative feature that serves the same, equivalent, or similar purpose. Therefore, unless otherwise specified, the features disclosed are merely general examples of equivalent or similar features.

[0107] Preparation method of krill oil microcapsule powder

[0108] The technical route for preparing krill oil microcapsule powder of the present invention is as follows: Figure 1 shown.

[0109] (1) Prepare the aqueous phase: Take pure water, heat it to 60-80°C in a water bath, transfer it to a blender, add the above-mentioned aqueous phase reagents (wall materials) while stirring, stir to mix, and cool to 30-55°C;

[0110] (2) Preparation of the oil phase: Place the desired krill oil in a beaker in a water bath at 40-70°C, transfer to a blender, add mono- and diglyceride fatty acids (emulsifier, powder) while stirring, and mix thoroughly to obtain the oil phase;

[0111] (3) Shearing: The water phase is moved to a shearing machine for shearing, and the oil phase is slowly added while shearing;

[0112] (4) Homogenization;

[0113] (5) Spray drying to obtain krill oil microcapsule powder.

[0114] The main advantages of the present invention include:

[0115] (1) The krill oil microcapsule powder of the present invention has the characteristics of high embedding efficiency, high oil loading, good product stability, high bioavailability, and uniform particle size;

[0116] (2) The embedding efficiency of the krill oil microcapsule powder of the present invention is ≥95%;

[0117] (3) The oil loading of the krill oil microcapsule powder of the present invention is ≥20%;

[0118] (4) The krill oil microcapsule powder of the present invention has good resolubility;

[0119] (5) The krill oil microcapsule powder of the present invention has no fishy smell;

[0120] (6) The krill oil microcapsule powder of the present invention has a higher blood absorption rate than krill oil of equal concentration and dosage, and has higher DHA and EPA.

[0121] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended to illustrate the present invention only and are not intended to limit the scope of the invention. The experimental methods in the following examples, for which specific conditions are not specified, are generally based on conventional conditions, such as those described in Sambrook et al., Molecular Cloning: A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are weight percentages and parts by weight.

[0122] Reagents, instruments and experimental animals

[0123] Reagents: Antarctic krill oil (Shandong Luhua Marine Biotechnology Co., Ltd.); sodium caseinate (Shanghai Titan Technology Co., Ltd.); maltodextrin (Shanghai Shisheng Xibas Technology Development Co., Ltd.); mono- and diglycerides of fatty acids (Jiangsu Yuanzhiyuan Biotechnology Co., Ltd.); gelatin (Shanghai Shisheng Xibas Technology Development Co., Ltd.); petroleum ether (Sinopharm Chemical Reagent Co., Ltd.); n-hexane (Sinopharm Chemical Reagent Co., Ltd.); chloroform (Sinopharm Chemical Reagent Co., Ltd.); sodium starch octenylsuccinate (Roquette Nutrition Food Co., Ltd.); sodium carboxymethyl cellulose (CMC-Na) (Shanghai Changguang Enterprise Development Co., Ltd.)

[0124] Instruments: Water bath (Shanghai Titan Technology Co., Ltd.); stirrer (Aika (Guangzhou) Instrument Equipment Co., Ltd.); homogenizer (Spix Fluids Co., Ltd.); shear (Shanghai Fluke Technology Development Co., Ltd.); spray dryer (Buchi Laboratory Equipment Trading (Shanghai) Co., Ltd.); Soxhlet extractor (Shanghai Titan Technology Co., Ltd.); electric blast drying oven (Shanghai Yiheng Scientific Instrument Co., Ltd.); potentiometric particle size analyzer (Shanghai Spectris Instrument Systems Co., Ltd.); magnetic stirrer (Beijing Southeast Yicheng Laboratory Equipment Co., Ltd.)

[0125] Eight-week-old male C57BL / 6 mice were purchased from Beijing Weitonglihua Laboratory Animal Technology Co., Ltd. (Shanghai Branch).

[0126] Preparation Example

[0127] Example 1

[0128] 400g of pure water was placed in a 1000ml container and heated in a water bath at 70°C. 50g of sodium caseinate, 10g of gelatin, and 80g of maltodextrin were added to the water while stirring (500 rpm). After uniform mixing, the aqueous phase was obtained and cooled to 50°C. 60g of Antarctic krill oil was placed in a 250ml beaker and heated in a 50°C water bath to obtain the oil phase. This oil phase mixture was slowly poured into the aqueous phase and sheared at 50°C and 10,000 rpm for 15 minutes. The mixture was then homogenized twice at 35 MPa to obtain an emulsion. The emulsion was spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder was collected in an aluminum foil bag and sealed.

[0129] Example 2

[0130] 800g of pure water was placed in a 1000ml container and heated in a 70°C waterbath. 50g of sodium caseinate, 10g of gelatin, and 100g of maltodextrin were added to the water while stirring (500 rpm). After uniform mixing, the mixture was cooled to 50°C. 40g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C waterbath to obtain the oil phase. This oil phase mixture was slowly poured into the aqueous phase. After shearing at 50°C and 10,000 rpm for 15 minutes, the mixture was homogenized twice at 35 MPa to obtain an emulsion. The emulsion was pressure spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the mixture was passed through a 50-mesh sieve and the krill oil microencapsulated powder product was collected in an aluminum foil bag and sealed.

[0131] Example 3

[0132] 800g of pure water was placed in a 1000ml container and heated in a 70°C water bath. 50g of sodium caseinate, 10g of gum arabic, and 100g of maltodextrin were added to the pure water while stirring (500 rpm). After uniform mixing, the aqueous phase was obtained and cooled to 50°C. 40g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. The oil phase mixture was slowly poured into the aqueous phase and sheared at 50°C and 10,000 rpm for 15 minutes. The mixture was then homogenized twice under 35 MPa to obtain an emulsion. The emulsion was spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder product was collected in an aluminum foil bag and sealed.

[0133] Example 4

[0134] 800g of pure water was placed in a 1000ml container and heated in a 70°C water bath. 50g of sodium caseinate, 10g of gelatin, and 100g of resistant dextrin were added to the pure water while stirring (500 rpm). After uniform mixing, the aqueous phase was obtained and cooled to 50°C. 40g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. The oil phase was slowly poured into the aqueous phase and sheared at 50°C and 10,000 rpm for 15 minutes. The emulsion was then homogenized twice under 35 MPa to obtain an emulsion. The emulsion was spray dried using a pressure spray method at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder product was collected in an aluminum foil bag and sealed.

[0135] Example 5

[0136] 800g of pure water was placed in a 1000ml container and heated in a water bath at 70°C. 50g of sodium caseinate, 10g of sodium starch glycolate octenylsuccinate, and 120g of resistant dextrin were added to the water while stirring (500 rpm). After uniform mixing, the aqueous phase was obtained and cooled to 50°C. 20g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. This oil phase was slowly poured into the aqueous phase and sheared at 50°C and 10,000 rpm for 15 minutes. The emulsion was then homogenized twice at 35 MPa to obtain an emulsion. The emulsion was spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder was collected in an aluminum foil bag and sealed.

[0137] Example 6

[0138] 800g of pure water was placed in a 1000ml container and heated in a water bath at 70°C. 50g of sodium caseinate, 10g of gelatin, and 90g of resistant dextrin were added to the pure water while stirring (500 rpm). After uniform mixing, the aqueous phase matrix was obtained and cooled to 50°C. 50g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. This oil phase mixture was slowly poured into the aqueous phase matrix and sheared at 50°C and 10,000 rpm for 15 minutes. After homogenization at 35 MPa, the emulsion was homogenized twice to obtain an emulsion. The emulsion was spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder product was collected in an aluminum foil bag and sealed.

[0139] Example 7

[0140] Place 500g of pure water in a 1000ml container and heat in a 70°C waterbath. Add 40g of sodium caseinate, 89g of resistant dextrin, and 3g of potassium citrate to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 60g of Antarctic krill oil in a 100ml beaker and heat in a 50°C waterbath. Add 4g of the antioxidant ascorbyl palmitate and 4g of the emulsifiers mono- and diglycerides while stirring (500 rpm). Stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0141] Example 8

[0142] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 126g of maltodextrin, and 4g of gum arabic to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 20g of mono- and diglycerol fatty acid emulsifiers while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0143] Example 9

[0144] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 20g of sodium caseinate and 130g of maltodextrin to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 10g of mono- and diglycerol fatty acid emulsifier while stirring (500r / min). Stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0145] Example 10

[0146] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 20g of sodium caseinate, 126g of maltodextrin, and 4g of gum arabic to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 10g of mono- and diglycerol fatty acid emulsifier while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0147] Example 11

[0148] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate and 144g of maltodextrin to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 6g of mono- and diglycerol fatty acid emulsifier while stirring (500r / min). Stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0149] Example 12

[0150] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 142g of maltodextrin, and 2g of gum arabic to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 6g of mono- and diglycerol fatty acid emulsifiers while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0151] Example 13

[0152] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 116g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 70g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerol fatty acid emulsifiers while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0153] Example 14

[0154] Place 200g of pure water in a 500ml container and heat it in a waterbath at 70°C. Add 20g of sodium caseinate, 134g of maltodextrin, and 4g of sodium starch glycolate octenylsuccinate to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerides of fatty acids (an emulsifier) while stirring (500r / min) to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0155] Example 15

[0156] 200g of pure water was placed in a 500ml container and heated in a water bath at 70°C. 10g of sodium caseinate and 150g of maltodextrin were added to the pure water while stirring (500r / min). After stirring, the aqueous phase was obtained and cooled to 50°C. 40g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. The oil phase was slowly poured into the aqueous phase and sheared at 50°C and 10,000r / min for 15 minutes. The emulsion was then homogenized twice under 35MPa to obtain an emulsion. The emulsion was spray dried by pressure at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added according to the weight of the powder. After mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder product was collected in an aluminum foil bag and sealed.

[0157] Example 16

[0158] Place 200g of pure water in a 500ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate and 148g of maltodextrin to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerides of fatty acids (an emulsifier) while stirring (500r / min) to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0159] Example 17

[0160] 200g of pure water was placed in a 500ml container and heated in a water bath at 70°C. 10g of sodium caseinate, 148g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate were added to the water while stirring (500 rpm). After uniform mixing, the aqueous phase was obtained and cooled to 50°C. 40g of Antarctic krill oil was placed in a 100ml beaker and heated in a 50°C water bath to obtain the oil phase. The oil phase was slowly poured into the aqueous phase and sheared at 50°C and 10,000 rpm for 15 minutes. The emulsion was then homogenized twice at 35 MPa to obtain an emulsion. The emulsion was spray dried at a flow rate of 20mL / min, an inlet air temperature of 180°C, and an outlet air temperature of 100-120°C. The powder was collected and 0.3% food-grade silica was added based on the weight of the powder. After uniform mixing, the powder was passed through a 50-mesh sieve and the krill oil microencapsulated powder was collected in an aluminum foil bag and sealed.

[0161] Example 18

[0162] Place 200g of pure water in a 500ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 144g of maltodextrin, and 4g of sodium starch glycolate octenylsuccinate to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerides of fatty acids (an emulsifier) while stirring (500r / min) to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0163] Example 19

[0164] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 136g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 50g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerol emulsifiers while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0165] Example 20

[0166] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 126g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 60g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerol fatty acid emulsifiers while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0167] Example 21

[0168] Place 200g of pure water in a 500ml container and heat it in a waterbath at 70°C. Add 20g of sodium caseinate, 136g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerides of fatty acids (an emulsifier) while stirring (500r / min) to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0169] Example 22

[0170] Place 400g of pure water in a 1000ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 166g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500 rpm). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 20g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerol fatty acid emulsifier while stirring (500 rpm) and stir thoroughly to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000 rpm for 15 minutes, and homogenize twice at 35 MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0171] Example 23

[0172] Place 400g of pure water in a 500ml container and heat it in a waterbath at 70°C. Add 10g of sodium caseinate, 146g of maltodextrin, and 2g of sodium starch glycolate octenylsuccinate to the water while stirring (500r / min). Stir thoroughly to obtain the aqueous phase matrix, which is then cooled to 50°C. Place 40g of Antarctic krill oil in a 100ml beaker and heat it in a 50°C waterbath. Add 2g of mono- and diglycerides of fatty acids (an emulsifier) while stirring (500r / min) to obtain the oil phase mixture. Slowly pour this oil phase mixture into the aqueous phase matrix, shear at 50°C and 10,000r / min for 15 minutes, and homogenize twice at 35MPa to obtain an emulsion. The emulsion was spray dried by pressure spray drying at a flow rate of 20 mL / min, an air inlet temperature of 180° C., and an air outlet temperature of 100-120° C. The powder was collected, and 0.3% food-grade silicon dioxide was added according to the weight of the powder. After mixing evenly, the powder was passed through a 50-mesh sieve and the krill oil microcapsule powder product was collected in an aluminum foil bag and sealed.

[0173] Table 1 shows the weight percentages of the components of the krill oil microcapsule powders of Examples 1 to 23.

[0174] Table 1

[0175]

[0176] Effect Examples

[0177] Example 24: Encapsulation efficiency, potential and particle size of krill oil microcapsule powder.

[0178] 24.1 Methods

[0179] (1) Determination of surface oil content of microcapsule powder

[0180] Accurately weigh 2 g of the krill oil microcapsule powder prepared in Example 23, add 20 mL of a 4:1 volume ratio of n-hexane to chloroform, shake rapidly, and centrifuge at 3000 rpm for 2 minutes. Then, remove 10 mL of the supernatant using a pipette and place it into a weighed single-necked flask. Recover the solvent until it is completely evaporated. The added mass of the flask, W1, is equivalent to the mass of surface oil in 1 g of microcapsules. The surface oil content of the microcapsules is represented by F, where F = W1 × 100%.

[0181] (2) Determination of total oil content of microcapsule powder:

[0182] The total oil content of the microcapsules (Example 23) was extracted using the Soxhlet extraction method, where W2 represents the total oil content in 2 g of microcapsules. T represents the total oil content of the microcapsules, and W represents the theoretical mass of Antarctic krill oil in a 2 g microcapsule sample. Thus, T = (W2 / W) x 100%.

[0183] (3) Calculation of microcapsule embedding rate:

[0184] Microcapsule embedding efficiency (%) = (TF) / T × 100%. Where: T is the total oil content of microcapsules; F is the oil content on the surface of microcapsules.

[0185] 24.2 Results

[0186] Table 2 shows the encapsulation efficiency, potential and particle size of the krill oil microcapsule powder prepared in Examples 1 to 23.

[0187] Table 2

[0188] Grouping Embedding rate Potential (mV) Particle size (nm) Example 1 56.6％ - - Example 2 75.3％ - - Example 3 78.7％ - - Example 4 79.9％ - - Example 5 82.6％ - - Example 6 70.4％ -45.85 263.8 Example 7 75.6％ -42.47 229.828 Example 8 52.8％ - - Example 9 63.5％ - - Example 10 65.9％ - - Example 11 72.3％ - - Example 12 75.8％ - - Example 13 63.80％ - - Example 14 95.90％ - - Example 15 87.60％ -40.81 235.8 Example 16 87.90％ -47.07 241.4 Example 17 92.90％ -42.98 208.9 Example 18 88.40％ -41.13 223.6 Example 19 87.50％ - - Example 20 85.60％ - - Example 21 87.50％ - - Example 22 93％ - - Example 23 97％ -44.55 220.4

[0189] Note: “-” means not tested.

[0190] Table 2 shows that the krill oil microcapsule powder prepared using 5% sodium caseinate, 73% maltodextrin, 1% mono- and diglycerides of fatty acids, and 1% sodium starch octenylsuccinate exhibits optimal potential, particle size, and encapsulation efficiency. Therefore, the krill oil microcapsule powder prepared in Example 23 was used as a sample for subsequent experiments.

[0191] Example 25 Redissolution stability of krill oil microcapsule powder

[0192] 25.1 Methods

[0193] Take 5g of krill oil microcapsule powder and put it into 150mL of water. After stirring to dissolve, pour it into a 150mL sealed bottle and maintain it at 95℃ for 10 minutes. Place the bottle upside down. The microcapsule powder can form a stable emulsion with water. Place it at room temperature for 6 months to observe whether the krill oil microcapsule powder has any oil floating, precipitation, or sticking to the bottle.

[0194] 25.2 Results

[0195] The particle size and potential of the krill oil microcapsule powder changed minimally before and after reconstitution, indicating a relatively stable overall system. Furthermore, after reconstitution, the emulsion showed no signs of oil scattering, sedimentation, or bottle sticking even after six months of storage.

[0196] Table 3 shows the particle size and potential value of the krill oil microcapsule powder prepared in Example 23 before and after reconstitution.

[0197] Table 3 Particle size and potential value of emulsion before and after reconstitution of microcapsule powder

[0198] Temperature (℃) Microcapsule powder emulsion before reconstitution Emulsion after reconstitution of microcapsule powder Particle size (nm) 217.4 269.1 Potential (mV) -36.02 -27.01

[0199] Example 26 Thermal Stability of Krill Oil Microcapsule Powder

[0200] 26.1 Methods

[0201] Krill oil microcapsules were dissolved in deionized water at 25, 62, and 100°C to a final concentration of 0.05 mg / mL. Heat in a water bath for 30 minutes and cool to room temperature. Particle size and potential parameters were measured.

[0202] 26.2 Results

[0203] The particle size and potential of the microcapsule powder did not change much after being treated at different temperatures, indicating that it has good heat resistance after heat treatment and can be used in beverages such as milk powder, yogurt and beverages.

[0204] Table 4 shows the particle size and potential of the krill oil microcapsule powder prepared in Example 23 after being treated at 25, 62 and 100°C.

[0205] Table 4 Particle size and potential after heat treatment

[0206] Temperature (℃) 25 62 100 Particle size (nm) 209.3 219.6 251 Potential (mV) -30.98 -32.45 -35.38

[0207] Example 27 pH Stability of Krill Oil Microcapsule Powder

[0208] 27.1 Methods

[0209] Dissolve the microcapsule powder in 62°C water to a concentration of 0.05 mg / mL. Adjust the pH of the sample to the pH shown in Table 3 by adding 0.01–2 mol / L of HCl or NaOH. Stir continuously for 1 hour using a magnetic stirrer at 500 rpm. Analyze parameters such as particle size and potential.

[0210] 27.2 Results

[0211] The particle size and potential of the microcapsule powder did not change much after being treated with different pH values, indicating that it has good stability in both acidic and alkaline environments and can be used in acidic or alkaline foods.

[0212] Table 5 shows the particle size and potential of the krill oil microcapsule powder prepared in Example 23 after 1 hour at different pH values.

[0213] Table 5 Particle size and potential after treatment with different pH values

[0214] pH 2 3 4 5 6 7 8 9 Particle size 276.3 257.4 302.3 227.3 248.4 200.1 265.5 304.1 Potential -29.29 -37.8 -36.16 -33.4 -48.66 -34.34 -25.5 -31.86

[0215] Example 28 Pharmacokinetic Test of Krill Oil Microcapsule Powder in Mice

[0216] 28.1 Methods

[0217] After one week of adaptive feeding, the mice were randomly divided into two groups, each with four mice. They were gavaged with krill oil and krill oil microcapsule powder (Example 23) containing the same concentration (0.57 g / kg) of omega-3 unsaturated fatty acids (EPA+DHA) (i.e., the krill oil dose was 3 g / kg (concentration 0.15 g / mL) and the krill oil microcapsule powder dose was 15 g / kg (concentration 0.75 g / mL). Blood was collected from the mice 1, 2, 4, and 8 hours after the gavage treatment, and the changes in the blood concentrations of EPA and DHA in the mice of the different gavage treatment groups were measured.

[0218] 28.2 Results

[0219] Depend on Figure 2 、 Figure 3 As shown in Table 6, by detecting the EPA and DHA absorption into the blood of mice at 1 hour and 8 hours, the EPA and DHA contents of mice treated with krill oil microcapsule powder by gavage were higher than those of mice treated with krill oil at the same concentration.

[0220] like Figure 3 As shown in the results, within 8 hours, the cumulative amount of DHA in mice treated with krill oil microcapsules by oral gavage was higher than that in mice treated with krill oil at the same concentration.

[0221] Therefore, krill oil microcapsule powder can increase the content of DHA and EPA in mice.

[0222] The present inventors speculate that the above results are due to the effect of the aqueous solution, which causes it to dissolve and absorb quickly.

[0223] Table 6 DHA and EPA in the blood of mice

[0224]

[0225]

[0226] discuss

[0227] This study measured the particle size, potential, embedding efficiency, and blood pharmacokinetic performance of Antarctic krill oil microcapsules prepared using different wall material ratios in mice. The results showed that the krill oil microcapsules prepared using a wall material composition consisting of 5% sodium caseinate, 73% maltodextrin, 1% mono- and diglycerides of fatty acids, and 1% sodium starch octenylsuccinate achieved optimal potential, particle size, and embedding efficiency. Mice were treated with krill oil microcapsules prepared using this wall material ratio to investigate the pharmacokinetic effects of krill oil and krill oil microcapsules in blood. Two groups of mice were treated with krill oil and krill oil microcapsules, and changes in blood drug concentrations in each group were measured and analyzed. The results showed that the blood EPA and DHA levels of krill oil microcapsules were essentially the same as those of krill oil, with no statistically significant differences.

[0228] Compared with a certain microencapsulation process that has been applied for and published, the present invention differs in experimental design and results: First, in terms of raw material production, the microencapsulation production process of fish oil, algae oil, vegetable fat powder, etc. is relatively complex, and the aqueous wall materials used are β-cyclodextrin, oligomaltose, and modified starch. The wall materials used in the present invention are sodium caseinate, maltodextrin, mono- and diglyceride fatty acids, and sodium starch octenylsuccinate, which have a simple formula, a simple preparation process, and are easy to operate. Secondly, the present invention uses an animal blood pharmacokinetic test to verify the bioavailability of krill oil microcapsule powder, which is not available in other patents. The present invention uses a mouse animal experiment to verify the blood pharmacokinetic test of krill oil microcapsule powder, and the results show that the EPA and DHA content of krill oil microcapsule powder in the blood is basically the same as that of krill oil, with no statistically significant difference. The DHA absorption rate of krill oil microcapsule powder into the blood is slightly higher than that of krill oil, which may be due to the effect of the aqueous solution, which allows it to dissolve and absorb quickly.

[0229] In summary, krill oil microcapsule powder prepared with a specific ratio of wall materials can achieve high encapsulation rate, good stability, high oil loading capacity, uniform particle size, and high production efficiency. Combined with animal experiments, it was shown that the EPA and DHA content of krill oil microcapsule powder entering the blood is basically the same as that of krill oil, with no statistically significant difference.

[0230] The present invention can be used to improve the embedding rate of Antarctic krill oil, achieve the characteristics of high embedding rate, good stability, high oil loading, uniform particle size, high production efficiency, etc. of Antarctic krill oil, and solve the shelf life problem of Antarctic krill oil.

[0231] The method of the present invention can be applied to the development of products such as baking, solid beverages, health foods, dairy products, capsule products, and special medical foods.

[0232] The preparation of microencapsulated powder of krill oil derived from Antarctic krill has broad market and development potential, and the microencapsulated powder prepared by the present invention has higher encapsulation efficiency and oil loading than other methods. Furthermore, existing patents for the preparation of Antarctic krill oil microencapsulated powder use single testing parameters and lack visual data, which makes microencapsulated powder stability testing lacking in certain persuasiveness. The Antarctic krill oil microencapsulated powder prepared by the present invention can achieve an encapsulation efficiency of up to 97%. Furthermore, a multi-faceted, multi-level validation approach was employed to verify the stability of the Antarctic krill oil microencapsulated powder, including tests on reconstitution stability, thermal stability, pH stability, and microencapsulated powder encapsulation efficiency. Furthermore, a pharmacokinetic study in mice confirmed that the blood entry rate and retention rate of the Antarctic krill oil microencapsulated powder were substantially consistent with those of krill oil. Furthermore, the DHA / EPA in krill oil has higher bioavailability than fish oil and algae oil. Therefore, research is underway to address the stability, water solubility, acid and alkali resistance, low bioavailability, and odor issues associated with encapsulating functional oils such as krill oil DHA / EPA. Furthermore, efforts are underway to address the compatibility of krill oil microencapsulated powder with functional foods, as well as the issue of breakage and leakage during processing, which can lead to severe odors. This ensures that the product's taste and flavor remain unchanged, ensuring consumer preference.

[0233] All documents mentioned in this application are incorporated herein by reference, just as if each document were incorporated herein by reference individually. It should also be understood that after reading the above teachings of the present invention, those skilled in the art may make various changes or modifications to the present invention, and that such equivalents also fall within the scope of the claims appended hereto.

Claims

1. A krill oil microcapsule powder, characterized in that: include: (a) an active ingredient, the active ingredient comprising krill oil; (b) sodium caseinate; and (c) a bulking agent, the bulking agent comprising maltodextrin; Furthermore, the embedding rate of the krill oil microcapsule powder is ≥80%.

2. The krill oil microcapsule powder according to claim 1, wherein: The krill oil microcapsule powder further comprises a thickener and / or an emulsifier.

3. The krill oil microcapsule powder according to claim 2, wherein: The thickener is selected from the group consisting of sodium starch octenylsuccinate, gum arabic, gelatin, or a combination thereof.

4. The krill oil microcapsule powder according to claim 2, wherein: The emulsifier includes mono- and di-glyceride fatty acids.

5. The krill oil microcapsule powder according to claim 1, wherein: The krill oil microcapsule powder has the following components in parts by weight:

6. The krill oil microcapsule powder according to any one of claims 1 to 5, characterized in that: The DHA concentration P1 in the blood of the subject who administered the krill oil microcapsule powder is compared with the DHA concentration P0 in the blood of the subject who administered krill oil, and the ratio (P1 / P0) is ≥1.0; the EPA concentration Z1 in the blood of the subject who administered the krill oil microcapsule powder is compared with the EPA concentration Z0 in the blood of the subject who administered krill oil, and the ratio (Z1 / Z0) is ≥1.

0.

7. The krill oil microcapsule powder according to any one of claims 1 to 5, characterized in that: The oil loading amount of the krill oil microcapsule powder is ≥15%.

8. A method for preparing the krill oil microcapsule powder according to any one of claims 1 to 7, characterized in that: The method comprises the steps of: (s1) providing krill oil, sodium caseinate and fillers; (s2) krill oil, sodium caseinate and maltodextrin are mixed, sheared, homogenized and dried to obtain krill oil microcapsule powder.

9. The method according to claim 8, wherein The bulking agent includes maltodextrin, resistant dextrin, or a combination thereof.

10. A use of the krill oil microcapsule powder according to any one of claims 1 to 7, characterized in that: Used in the preparation of food additives, baked goods, solid beverages, health foods, dairy products, capsule products, or special medical foods.

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