Composition with anti-wrinkle and firming effects and application thereof

Abstract

The invention provides a composition with anti-wrinkle and firming effects and application of the composition. The composition with the anti-wrinkle and firming effects comprises sterols, nicotinamide and arginine / lysine polypeptide. The sterols comprise PEG-10 (Polyethylene Glycol) wild soybean sterols. According to the invention, nicotinamide, arginine / lysine polypeptide and sterols are creatively combined into a brand-new composition, and the three components have excellent performance in anti-wrinkle and firming under the condition of lower content and have potential mutual promotion effect; particularly, the PEG-10 wild soybean sterol can play a role in promoting the anti-wrinkle and firming synergistic effect of the nicotinamide and the arginine / lysine polypeptide, and the nicotinamide, the arginine / lysine polypeptide and the PEG-10 wild soybean sterol improve the elasticity and the firming degree of the skin by promoting I-type collagen gene expression and elastin expression, enhancing the oxidation resistance and promoting the elastase inhibition rate.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily cosmetics, and in particular relates to a composition with anti-wrinkle and firming effects and an application thereof. Background Art

[0002] In 2024, the global anti-aging market reached $266.2 billion and is projected to soar to nearly $500 billion by 2032, with a compound annual growth rate of 8%. In 2023, the Chinese anti-aging skincare market exceeded 150 billion yuan, accounting for 62% of the industry. In the anti-aging market, skincare products hold a dominant position with a 58% share. However, consumers' understanding of anti-aging has long gone beyond the simple "anti-wrinkle" and "firming" needs, turning to the deeper integration of cosmetics and medical aesthetics, as well as the integration of innovative technologies. Innovative methods such as non-invasive beauty effects and nano-transdermal technology can significantly improve the absorption rate of active ingredients, which are issues that consumers value.

[0003] CN119280102A discloses a skin care composition with anti-aging effects, its preparation method and application, belonging to the field of beauty and skin care technology. In terms of the combination of ingredients, retinol, polypeptide complex, ceramide, astaxanthin and resveratrol work synergistically to fight aging in all directions, each of which plays a role in promoting collagen production, solving muscle contraction wrinkles, enhancing barrier function, scavenging free radicals, inhibiting melanin formation, etc. In terms of preparation method, advanced technologies such as precise temperature control, appropriate stirring mode, high-precision stirrer, etc. ensure uniform mixing of ingredients, inert gas protection and PID temperature controller retain the activity and efficacy of ingredients. In terms of application, it can be reasonably added to creams, lotions, essences and masks to meet diverse needs, providing deep nourishment, refreshing care, efficient penetration and immediate effects respectively. In short, it provides consumers with an efficient, safe and comprehensive anti-aging solution to improve signs of aging, with broad prospects and high value.

[0004] CN116763683A discloses an anti-aging skin composition and its use. Its ingredients primarily include: a solvent, a moisturizer, oxidized tannic acid, oxidized linoleic acid, a skin conditioner, an antioxidant, a thickener, a solubilizer, and a fragrance. This invention optimizes the formulation of the anti-aging skin composition. By precisely combining multiple active ingredients for synergistic anti-aging effects, it fully leverages the skincare benefits of each raw material component. The combination of multiple active ingredients enhances the anti-aging effect, significantly increasing the skincare efficacy. The invention introduces oxidized tannic acid and oxidized linoleic acid into the anti-aging skin composition, allowing them to interact with collagen, another component, to form a stable network, enhancing the antioxidant activity and emulsion stability of the composition.

[0005] Although the above-mentioned anti-aging products with different mechanisms of action can improve skin sagging and other problems to a certain extent, their active ingredients generally have problems such as poor stability, poor permeability, and difficulty in absorption. Therefore, it is very necessary and there is a huge market demand to provide a product with better permeability of active ingredients and more significant efficacy. Summary of the Invention

[0006] In view of the deficiencies of the prior art, the present invention provides a composition with anti-wrinkle and firming effects and its application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises sterols, niacinamide, and arginine / lysine polypeptide;

[0009] The sterols include PEG-10 soja sterol.

[0010] The PEG-10 soybean sterol in the composition of the present invention is a nonionic surfactant with a unique amphiphilic structure. It can not only improve the stability of the formula but also promote the penetration of active ingredients. This ingredient not only has excellent skin conditioning effects, but also its unique amphiphilic structure can achieve optimal penetration of water-soluble niacinamide and small molecule peptides. This is because intercellular lipids and cell membranes are oil-soluble components, and soybean sterol is a sterol substance whose basic structure includes a four-ring steroid nucleus and several side chains. These structures have strong lipophilicity and can penetrate better than a simple fully water-soluble amino acid sequence.

[0011] Nicotinamide is a water-soluble vitamin that can participate in the energy metabolism process of cells, regulate the redox state in cells, thereby reducing the generation of free radicals, stimulate the synthesis of collagen in skin fibroblasts, inhibit the activity of MMPs, and reduce the degradation of elastin, thereby maintaining the elasticity of the skin and achieving anti-wrinkle and firming effects.

[0012] The arginine / lysine peptide, primarily composed of two basic amino acids, arginine and lysine, can target and block voltage-dependent sodium channels. This tightly interacts with the target, effectively blocking neuromuscular electrical conduction, instantly relaxing facial skin and restoring skin elasticity. Arginine / lysine peptides regulate neuromuscular interactions to block voltage-dependent sodium channels. This tight interaction, particularly targeting the muscle channel NAV1.4, effectively blocks neuromuscular electrical conduction, relaxes muscles, and instantly reduces wrinkles and enhances firmness. A relatively high concentration is required for their effectiveness on the human body.

[0013] The present invention creatively combines niacinamide, arginine / lysine polypeptide and sterols into a new composition. The three ingredients have excellent anti-wrinkle and firming performance at relatively low levels and have potential to promote each other.

[0014] Preferably, the composition with anti-wrinkle and firming effects comprises, by weight, 0.01-10 parts of sterols, 0.1-25 parts of niacinamide, and 0.01-15 parts of arginine / lysine polypeptide.

[0015] In the anti-wrinkle and firming composition of the present invention, the added amount of the sterols can be 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0016] The amount of nicotinamide added can be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts or 24 parts, etc.;

[0017] The added amount of the arginine / lysine polypeptide can be 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.

[0018] Preferably, the composition with anti-wrinkle and firming effects comprises, by weight, 0.5-5 parts of sterols, 1-20 parts of niacinamide, and 0.1-10 parts of arginine / lysine polypeptide.

[0019] Preferably, the sterols further include cholesterol, rape sterols and phytosterol oleate.

[0020] Any specific point value within the above numerical range can be selected and will not be described in detail here.

[0021] In a second aspect, the present invention provides a use of the composition with anti-wrinkle and firming effects according to the first aspect in the preparation of cosmetics.

[0022] Preferably, the cosmetics include any one of essence, facial mask, cream, lotion or skin care water.

[0023] Preferably, the mass percentage of the composition with anti-wrinkle and firming effects in the cosmetic is 1-15%, for example, it can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%.

[0024] Any specific point value within the above numerical range can be selected and will not be described in detail here.

[0025] In a third aspect, the present invention provides an essence with anti-wrinkle and firming effects, wherein the components of the essence with anti-wrinkle and firming effects include the composition with anti-wrinkle and firming effects described in the first aspect, a moisturizer, a thickener, an antioxidant, an emollient and water.

[0026] Preferably, the components of the essence with anti-wrinkle and firming effects include, by weight, 1-10 parts of the composition with anti-wrinkle and firming effects described in the first aspect, 10-25 parts of moisturizer, 0.1-5 parts of thickener, 1-5 parts of antioxidant, 1-10 parts of emollient and 45-90 parts of water.

[0027] The amount of the composition with anti-wrinkle and firming effects added to the essence of the present invention can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0028] The amount of the moisturizer added can be 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts or 24 parts, etc.;

[0029] The amount of the thickener added can be 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts, etc.;

[0030] The amount of the antioxidant added can be 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts;

[0031] The amount of the emollient added can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0032] The amount of water added can be 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts or 85 parts, etc.

[0033] Preferably, the moisturizing agent includes any one of glycerin, butylene glycol, dipropylene glycol or glycereth-26, or a combination of at least two thereof.

[0034] Preferably, the thickener comprises any one or a combination of at least two of xanthan gum, hydroxyethyl cellulose, Sphingomonas fermentation product extract, carbomer, or acrylates / C10-30 alkyl acrylate crosspolymer.

[0035] Preferably, the antioxidant includes any one of p-hydroxyacetophenone, tocopherol or tocopherol acetate, or a combination of two thereof.

[0036] Preferably, the emollient comprises any one or a combination of at least two of caprylic / capric triglyceride, shea butter, coconut oil caprylate / caprate, dimethicone, isononyl isononanoate or hydrogenated polyisobutene.

[0037] Preferably, the emollient is a combination of caprylic / capric triglyceride, coconut caprylate / caprate, isononyl isononanoate and hydrogenated polyisobutene.

[0038] The emollient of the present invention can enhance the barrier function of the skin, improve the skin's resistance to the external environment, reduce the damage to the skin caused by external stimuli, and thus enhance the anti-wrinkle and firming effects of the skin through the synergistic combination of caprylic / capric triglyceride, coconut oil caprylate / caprate, isononyl isononanoate and hydrogenated polyisobutene.

[0039] Preferably, the mass ratio of caprylic / capric triglyceride, coconut oil caprylate / caprate, isononyl isononanoate and hydrogenated polyisobutene is (0.5-2): (1-4): (2-4): (1-3).

[0040] Wherein “0.5-2” can be 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0041] "1-4" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 or 3.8, etc.;

[0042] "2-4" can be 2.1, 2.3, 2.5, 2.8, 3, 3.2, 3.5, 3.7 or 3.9, etc.;

[0043] “1-3” can be 1.1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7 or 2.9, etc.

[0044] Preferably, the essence with anti-wrinkle and firming effects also includes a skin conditioner.

[0045] Preferably, the essence with anti-wrinkle and firming effects further comprises 0.1-5 parts by weight of a skin conditioner, for example, 0.2 parts, 0.5 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts or 4.5 parts.

[0046] Preferably, the skin conditioner composition comprises any one or a combination of at least two of dipotassium glycyrrhizate, allantoin, hyaluronic acid or sodium hyaluronate.

[0047] Preferably, the skin conditioning agent is a combination of dipotassium glycyrrhizate, allantoin and hyaluronic acid.

[0048] In the present invention, the hyaluronic acid can provide deep moisturizing and increase collagen synthesis, while allantoin can repair the epidermal barrier and accelerate cell renewal, reducing the activity of collagen-degrading enzymes. Dipotassium glycyrrhizate can inhibit oxidative damage and scavenge free radicals. The three together build a multi-layered protective network from the epidermis to the dermis, thereby better promoting the anti-wrinkle and firming effects of the essence.

[0049] Preferably, the mass ratio of dipotassium glycyrrhizate, allantoin and hyaluronic acid is (0.1-2): (0.1-1.5): (1-3).

[0050] Wherein “0.1-2” can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.;

[0051] “0.1-1.5” can mean 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3 or 1.4, etc.;

[0052] “1-3” can be 1.1, 1.3, 1.5, 1.8, 2, 2.2, 2.5, 2.7 or 2.9, etc.

[0053] Preferably, the essence with anti-wrinkle and firming effects further comprises any one of a preservative, a pH regulator or a fragrance, or a combination of at least two of them.

[0054] Any specific point value within the above numerical range can be selected and will not be described in detail here.

[0055] In a fourth aspect, the present invention provides a method for preparing the essence with anti-wrinkle and firming effects according to the third aspect, the preparation method comprising:

[0056] (1) Mixing a moisturizing agent, a thickener, an emollient, and water to obtain a mixed solution;

[0057] (2) The mixed solution obtained in step (1), the composition with anti-wrinkle and firming effects, and an antioxidant are mixed and homogenized to obtain the essence with anti-wrinkle and firming effects.

[0058] Preferably, the mixing temperature in step (1) is 60-70°C (for example, 61°C, 63°C, 65°C, 67°C or 69°C), and the mixing time is 5-10 min (for example, 6 min, 7 min, 8 min or 9 min).

[0059] Preferably, the mixing in step (1) further comprises adding a skin conditioner.

[0060] Preferably, the temperature of the mixing and homogenizing in step (2) is 35-45°C (for example, 36°C, 38°C, 40°C, 42°C or 44°C), and the mixing time is 20-40 min (for example, 22 min, 25 min, 28 min, 30 min, 32 min, 35 min or 38 min).

[0061] Preferably, step (2) further comprises adding any one or a combination of at least two of a preservative, a pH adjuster or a fragrance during the mixing and homogenization.

[0062] Any specific point value within the above numerical range can be selected and will not be described in detail here.

[0063] Compared with the prior art, the present invention has the following beneficial effects:

[0064] (1) The present invention creatively combines niacinamide, arginine / lysine peptides and sterols into a new composition. The three ingredients have excellent performance in anti-wrinkle and firming at low levels and have potential to promote each other. In particular, PEG-10 soybean sterol can promote the synergistic anti-wrinkle and firming effects of niacinamide and arginine / lysine peptides. The three improve the elasticity and firmness of the skin by promoting the expression of type I collagen gene, enhancing antioxidant capacity and promoting the inhibition rate of elastase.

[0065] (2) In the present invention, the hyaluronic acid can provide deep moisturizing, increase collagen synthesis, allantoin can repair the epidermal barrier and accelerate cell renewal, reduce the activity of collagen degrading enzymes, and dipotassium glycyrrhizate can inhibit oxidative damage and scavenge free radicals. The three together build a multi-level protection network from the epidermis to the dermis, thereby better promoting the anti-wrinkle and firming effect of the essence. DETAILED DESCRIPTION

[0066] The technical solution of the present invention is further described below by way of specific embodiments. It should be understood by those skilled in the art that the embodiments are merely to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0067] The sources of some raw materials in the examples or comparative examples of the present invention are shown in Table 1 below.

[0068] Table 1

[0069]

[0070] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0071] Example 1

[0072] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of niacinamide, and 5 parts of arginine / lysine polypeptide;

[0073] The sterols are a combination of PEG-10 soybean sterol, cholesterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0074] Example 2

[0075] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 1 part of sterols, 16 parts of niacinamide, and 1 part of arginine / lysine polypeptide;

[0076] The sterols are a combination of PEG-10 soybean sterol, cholesterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.5:0.3:0.5.

[0077] Example 3

[0078] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 5 parts of sterols, 5 parts of niacinamide, and 8 parts of arginine / lysine polypeptide;

[0079] The sterols are a combination of PEG-10 soybean sterol, cholesterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.3:0.8:0.2.

[0080] Example 4

[0081] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of niacinamide, and 5 parts of arginine / lysine polypeptide;

[0082] The sterol is PEG-10 soybean sterol.

[0083] Example 5

[0084] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of niacinamide, and 5 parts of arginine / lysine polypeptide;

[0085] The sterols are a combination of PEG-10 soybean sterol, cholesterol and rapeseed sterols in a mass ratio of 1:0.8:0.5.

[0086] Example 6

[0087] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of niacinamide, and 5 parts of arginine / lysine polypeptide;

[0088] The sterols are a combination of PEG-10 soybean sterol, cholesterol and phytosterol oleate in a mass ratio of 1:0.8:0.3.

[0089] Example 7

[0090] This embodiment provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols, 10 parts of niacinamide, and 5 parts of arginine / lysine polypeptide;

[0091] The sterols are a combination of PEG-10 soybean sterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.5:0.3.

[0092] Comparative Example 1

[0093] This comparative example provides a composition with anti-wrinkle and firming effects, which comprises 10 parts of niacinamide and 5 parts of arginine / lysine polypeptide in parts by weight.

[0094] Comparative Example 2

[0095] This comparative example provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises, by weight, 3 parts of sterols and 5 parts of arginine / lysine polypeptides;

[0096] The sterols are a combination of PEG-10 soybean sterol, cholesterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0097] Comparative Example 3

[0098] This comparative example provides a composition with anti-wrinkle and firming effects, wherein the composition with anti-wrinkle and firming effects comprises 3 parts of sterols and 10 parts of niacinamide in parts by weight;

[0099] The sterols are a combination of PEG-10 soybean sterol, cholesterol, rape sterols and phytosterol oleate in a mass ratio of 1:0.8:0.5:0.3.

[0100] Application Examples 1-3

[0101] This application example provides three essences with anti-wrinkle and firming effects. The components of the essences with anti-wrinkle and firming effects are shown in Table 2 below. The dosage of each component in the table is in parts:

[0102] Table 2

[0103]

[0104] The preparation method of the essence with anti-wrinkle and firming effects is as follows:

[0105] (1) Mixing a moisturizer, a thickener, an emollient, a skin conditioner, and deionized water at 65°C for 8 minutes to obtain a mixed solution;

[0106] (2) The mixed solution obtained in step (1), the composition with anti-wrinkle and firming effects, and the antioxidant are mixed and homogenized at 40° C. for 30 minutes to obtain the essence with anti-wrinkle and firming effects.

[0107] Application Examples 4-7

[0108] This application example provides four essences with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the composition with anti-wrinkle and firming effects provided in Example 1 is replaced with the compositions with anti-wrinkle and firming effects provided in Examples 4-7 in equal amounts. The remaining components and addition amounts are the same as those in Application Example 1. The preparation method refers to Application Example 1.

[0109] Application Example 8

[0110] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the emollient does not include caprylic / capric triglyceride, and its reduced amount is distributed according to the proportion of parts to coconut oil caprylate / caprate, isononyl isononanoate and hydrogenated polyisobutene. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0111] Application Example 9

[0112] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the emollient does not include coconut oil caprylate / caprate, and its reduced amount is distributed according to the proportion of parts to caprylic / capric triglyceride, isononyl isononanoate and hydrogenated polyisobutene. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0113] Application Example 10

[0114] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the emollient does not include isononyl isononanoate, and its reduced amount is distributed among caprylic / capric triglyceride, coconut oil caprylate / caprate and hydrogenated polyisobutene in proportion. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0115] Application Example 11

[0116] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the emollient does not include hydrogenated polyisobutene, and its reduced amount is distributed in proportion to caprylic / capric triglyceride, coconut oil caprylate / caprate and isononyl isononanoate. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0117] Application Example 12

[0118] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the essence does not include a skin conditioner, and the reduced amount is supplemented by deionized water. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0119] Application Example 13

[0120] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the skin conditioner does not include dipotassium glycyrrhizate, and its reduced amount is distributed to allantoin and hyaluronic acid in proportion. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0121] Application Example 14

[0122] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the skin conditioner does not include allantoin, and its reduced amount is distributed to dipotassium glycyrrhizate and hyaluronic acid in proportion. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0123] Application Example 15

[0124] This application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the skin conditioner does not include hyaluronic acid, and its reduced amount is distributed to dipotassium glycyrrhizate and allantoin in proportion. The remaining components and addition amounts are consistent with those in Application Example 1. The preparation method refers to Application Example 1.

[0125] Comparative Application Examples 1-3

[0126] This comparative application example provides three essences with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the composition with anti-wrinkle and firming effects provided in Example 1 is replaced by the compositions with anti-wrinkle and firming effects provided in Comparative Examples 1-3 in equal amounts. The remaining components and addition amounts are the same as those in Application Example 1. The preparation method refers to Application Example 1.

[0127] Comparative Application Example 4

[0128] This comparative application example provides an essence with anti-wrinkle and firming effects. The only difference from Application Example 1 is that the essence does not include a composition with anti-wrinkle and firming effects, and the reduced amount is supplemented by deionized water. The remaining components and added amounts are consistent with Application Example 1. The preparation method refers to Application Example 1.

[0129] Test Example 1 Antioxidant Efficacy Study - DPPH Free Radical Scavenging Experiment

[0130] Test samples: samples provided in Application Examples 1-15 and Comparative Application Examples 1-4;

[0131] Test method:

[0132] Accurately weigh 47.7 mg of DPPH reagent and dilute to a 100 ml brown volumetric flask. Take 5 ml of the solution and dilute to a 50 ml brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (47.7 mg / L). Pipette 2 mL of the DPPH test solution and add 0.1 mL of the sample provided in Application Examples 1-15 and Comparative Application Examples 1-4, respectively, as shown in Table 3 below. Then add an appropriate amount of anhydrous ethanol to equalize the total mass. After thorough mixing, let it stand in the dark for 30 minutes. Measure its absorbance at 517 nm using anhydrous ethanol as a blank control and vitamin C as a control. Calculate the DPPH scavenging rate of each extract according to the following formula:

[0133] DPPH clearance rate (%) = [1-(As-Ar) / A0] × 100%

[0134] Where: As is the absorbance of the reaction system of the extract and DPPH; Ar is the absorbance of the extract-anhydrous ethanol; A0 is the absorbance of the blank control anhydrous ethanol.

[0135] Table 3

[0136]

[0137] The DPPH free radical scavenging rate reflects, to a certain extent, its antioxidant capacity. A higher scavenging rate indicates a greater antioxidant capacity and, therefore, a greater anti-aging effect. Therefore, the anti-aging effect of an essence can be assessed by studying its DPPH free radical scavenging capacity. Vitamin C is recognized as a highly effective antioxidant in the cosmetics industry. This experiment used vitamin C as a control to examine its antioxidant capacity. The test results are shown in Table 4.

[0138] Table 4

[0139]

[0140] As shown in Table 4, compared with Comparative Application Example 4, the samples of Application Examples 1-3 all have better scavenging effects on DPPH, and the scavenging rates are all above 60%.

[0141] As shown in Application Examples 4-7, the anti-wrinkle and firming composition of the present invention can synergize with PEG-10 soybean sterol to enhance the antioxidant effect of the product by adding cholesterol, rape sterols, and phytosterol oleate, and there is a synergistic effect between the three.

[0142] However, the DPPH scavenging effect of Application Examples 12-15 was somewhat reduced, indicating that the addition of skin conditioning agents to the essence can improve the antioxidant effect of the product, and the synergistic combination of dipotassium glycyrrhizate, allantoin and hyaluronic acid can make the antioxidant effect of the product even better;

[0143] The DPPH scavenging effect in comparative application examples 1-3 decreased, indicating that the sterols, niacinamide and arginine / lysine polypeptide in the anti-wrinkle and firming composition of the present invention promote each other in terms of antioxidant effect and have a synergistic effect.

[0144] Test Example 2: Anti-wrinkle and skin firming test

[0145] The skin tightening effect of the essences prepared in Example 1-15 and Comparative Example 1-4 was evaluated:

[0146] 133 subjects aged 20-45 were randomly selected and divided into 19 groups of 7 subjects each. All 19 groups applied the essences prepared in Application Examples 1-15 and Comparative Application Examples 1-4 to their left and right faces (once in the morning and once in the evening) for 28 days. During the trial, subjects were prohibited from using any other products that could maintain skin elasticity or cosmetics that could affect the results. They also maintained their dietary habits.

[0147] Skin elasticity was measured on days 0 and 28 using a Cutometer MPA580. The test site was the zygomatic area. The probe was repeated three times in the same test area, and the average value was taken. The test parameter was R2 (the ratio of the skin's rebound value (Ua) without negative pressure to the maximum stretch (Uf) with negative pressure). The closer R2 is to 1, the better the skin's elasticity. The average R2 change value = R2 value on day 28 - R2 value on day 0. The average R2 change rate = R2 average change value / R2 value on day 0. The specific skin elasticity test results are shown in Table 5.

[0148] Table 5

[0149]

[0150] As shown in Table 5, compared with Comparative Application Example 4, the essences of Examples 1-3 of the present invention have better skin firming effects, indicating that the anti-wrinkle and firming compositions provided by the present invention can significantly improve the skin firming effects of the products. Furthermore, as shown in Comparative Application Examples 1-3, the three components, namely, sterols, niacinamide, and arginine / lysine polypeptides, mutually enhance the effects, resulting in an unexpected synergistic effect.

[0151] Test Example 3: Anti-wrinkle and firming test

[0152] The anti-wrinkle and firming effects of the samples prepared from Examples 1-15 and Comparative Examples 1-5 were evaluated:

[0153] The specific test method is as follows:

[0154] (1) Upregulation rate of type I collagen (COL1AI) and elastin (ELN) expression in human dermal fibroblasts:

[0155] Test Principle: Fibroblasts synthesize and secrete collagen and elastin, forming collagen fibers, reticular fibers, and elastic fibers. They also synthesize and secrete matrix components such as glycosaminoglycans and glycoproteins. Type I and II collagen are major components of the dermal extracellular matrix. In infant and adolescent skin, type I collagen accounts for approximately 70%. The appearance of skin wrinkles is closely related to the normal synthesis and expression of collagen. Elastin, present in the extracellular matrix, plays a vital role in maintaining skin elasticity and is a major contributor to aging phenomena such as skin laxity and sagging. Ultraviolet radiation can cause skin to become rough, thicken, and lose elasticity, leading to wrinkles. Skin fragility is partly attributed to changes in hemidesmosomes and downregulation of various collagen proteins (such as Collagen I) at the dermal-epidermal junction. In this study, using human dermal fibroblasts, the relative expression of COL1A1 and ELN genes was measured after the samples were applied to the cells to evaluate their anti-wrinkle and firming properties.

[0156] Experimental steps:

[0157] (1) Inoculate cells into 6-well plates (3x10 5 The cells were cultured at 37°C and 5% CO2 for 24 h.

[0158] (2) After the incubation, the culture medium was removed and washed 1-2 times with D-Hanks. The sample group and the model control group were then irradiated with UVA (9 J / cm²).

[0159] (3) After modeling, the normal control group and the model control group were replaced with fresh culture medium, and fresh culture medium containing samples was added to the sample wells, and the cells were incubated at 37°C and 5% CO2 for 24 h.

[0160] (4) Total RNA was extracted from each experimental group, cDNA was synthesized, and the gene expression of β-actin and target genes was detected by q-PCR.

[0161] (5) Using β-actin as an internal reference for gene expression, calculate the relative RNA expression of the target gene.

[0162] Relative RNA expression = 2 ΔΔC(t) ;

[0163] Where, ΔΔC(t) = ΔC(t) 模型对照组 -ΔC(t) 样品组 ;

[0164] ΔC(t) = C(t) 目的基因 -C(t) β-actin ;

[0165] Increase rate (%) = x100%

[0166] Where P: relative expression level of COL1AI and ELN genes

[0167] Statistical results are expressed as mean ± SD. This study used an independent sample T-test between two groups for statistical analysis. Statistical results were considered significant with a threshold of α = 0.05 and p < 0.05 (compared with the model control group). Compared with the model control group, the relative expression levels of COL1AI and ELN genes in the sample group were significantly upregulated, indicating anti-wrinkle and firming effects.

[0168] (2) Determination of anti-wrinkle and firming efficacy of elastase activity inhibition

[0169] Test Principle: Elastic fibers, composed of elastin and microfibrils, are distributed throughout the dermis and subcutaneous tissue, contributing to the skin's elasticity. Environmental factors such as UV radiation, stress, and pollution stimulate the production of elastase in the body. Elastase, a member of the chymotrypsin family, degrades elastin, leading to the loss of connective tissue in the epidermis and contributing to skin aging, wrinkles, and photoaging.

[0170] Through the experimental design, the sample group and the blank control group were compared to test the elastase inhibition rate. If the inhibition rate of the sample group is better (higher) than that of the blank control group and the statistical difference P value is less than 0.05, it can be considered that the sample group has certain anti-wrinkle and firming effects;

[0171] Test method:

[0172] (1) Weighing the sample;

[0173] (2) Pre-treating the sample group to prepare a mixed solution;

[0174] (3) Incubate at 37°C for a period of time; then add a certain amount of elastase and continue incubation;

[0175] (4) Test the OD value of elastase inhibition rate on the machine;

[0176] (5) Record data, calculate inhibition rate and statistical difference P value;

[0177] The specific test results are shown in Table 6 below:

[0178] Table 6

[0179]

[0180] As shown in Table 6, compared with Comparative Application Example 4, the essences of Application Examples 1-3 of the present invention increased the expression of type I collagen (COL1AI) by more than 80%, the expression of elastin (ELN gene) by more than 58%, and the inhibition rate of elastase by more than 28%, all of which were significantly improved. This shows that the composition with anti-wrinkle and firming effects provided by the present invention has significant anti-aging effects.

[0181] From the comparison of Application Examples 4-7, it can be seen that the anti-wrinkle and firming composition of the present invention can synergize with PEG-10 soybean sterol to better enhance the anti-aging effect of the product by adding cholesterol, rape sterols, and phytosterol oleate, and there is a synergistic effect between the three.

[0182] From the comparison of Application Examples 8-11, it can be seen that the selection of the emollient also affects the expression of type I collagen (COL1AI), the expression of elastin (ELN gene) and the inhibitory effect of elastase;

[0183] As can be seen from Application Example 1 and Application Examples 12-15, adding a skin conditioner to the essence of the present invention can make the product's anti-aging effect better, and the formula of the skin conditioner also affects the anti-aging effect of the essence;

[0184] From the comparison between Application Example 1 and Comparative Application Examples 1-3, it can be seen that the sterols, nicotinamide and arginine / lysine polypeptide have an unexpected synergistic effect in anti-aging efficacy.

[0185] Test Example 4: Safety Evaluation Experiment

[0186] The specific method is as follows: 40 women aged 18-35 years with no history of allergies were selected. Each subject was treated with the samples prepared in Examples 1-15. After cleaning the subject's back, a patch tester containing the sample was applied to the selected area on the back using non-irritating tape. After application, the patch was evenly applied to the skin using gentle pressure with fingers. This was maintained for 48 hours. The subject was instructed to keep the patch area dry for 48 hours and avoid strenuous exercise, scratching, or prolonged sunlight exposure. After 48 hours, the patch tester was removed and marked. After 30 minutes, once the indentation had disappeared, the test was performed under adequate lighting.

[0187] The grading standards for adverse skin reactions are shown in Table 7:

[0188] Table 7

[0189]

[0190] The results are shown in Table 8:

[0191] Table 8

[0192]

[0193] The data in Table 8 show that after removing the spot tester, no adverse reactions were observed on the skin of the volunteers. This indicates that the raw materials used in the essence of the present invention are safe and non-irritating, have no adverse reactions to the human body, and are highly safe.

[0194] The applicant states that while the above-described embodiments illustrate the anti-wrinkle and firming composition and its applications, the present invention is not limited to these embodiments. This does not necessarily mean that the present invention must rely on these embodiments in order to be implemented. Persons skilled in the art will appreciate that any improvements to the present invention, equivalent substitutions for raw materials in the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention.

[0195] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0196] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition with anti-wrinkle and firming effects, characterized in that: The composition with anti-wrinkle and firming effects comprises sterols, niacinamide and arginine / lysine polypeptide; The sterols include PEG-10 soja sterol.

2. The composition with anti-wrinkle and firming effects according to claim 1, characterized in that: In parts by weight, the composition with anti-wrinkle and firming effects comprises 0.01-10 parts of sterols, 0.1-25 parts of niacinamide, and 0.01-15 parts of arginine / lysine polypeptide; The sterols also include cholesterol, campesterols and phytosterol oleate.

3. Use of the composition with anti-wrinkle and firming effects according to claim 1 or 2 in the preparation of cosmetics.

4. The use according to claim 3, characterized in that The cosmetics include any one of essence, facial mask, cream, lotion or skin care water; The mass percentage of the composition with anti-wrinkle and firming effects in the cosmetics is 1-15%.

5. An essence with anti-wrinkle and firming effects, characterized in that: The components of the essence with anti-wrinkle and firming effects include the composition with anti-wrinkle and firming effects according to claim 1 or 2, a moisturizer, a thickener, an antioxidant, an emollient and water.

6. The essence with anti-wrinkle and firming effects according to claim 5, characterized in that: The components of the essence with anti-wrinkle and firming effects include, by weight, 1-10 parts of the composition with anti-wrinkle and firming effects according to claim 1 or 2, 10-25 parts of a moisturizer, 0.1-5 parts of a thickener, 1-5 parts of an antioxidant, 1-10 parts of an emollient, and 45-90 parts of water.

7. The anti-wrinkle and firming essence according to claim 5 or 6, characterized in that: The moisturizing agent includes any one of glycerin, butylene glycol, dipropylene glycol or glyceryl polyether-26, or a combination of at least two thereof; The thickener includes any one or a combination of at least two of xanthan gum, hydroxyethyl cellulose, Sphingomonas fermentation product extract, carbomer, or acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer; The antioxidant includes any one of p-hydroxyacetophenone, tocopherol or tocopherol acetate, or a combination of two thereof; The emollient is a combination of caprylic / capric triglyceride, coco-caprylate / caprate, isononyl isononanoate, and hydrogenated polyisobutene.

8. The anti-wrinkle and firming essence according to claim 5 or 6, characterized in that: The essence with anti-wrinkle and firming effects further comprises 0.1-5 parts by weight of a skin conditioning agent; The skin conditioning agent is a combination of dipotassium glycyrrhizate, allantoin and hyaluronic acid; The essence with anti-wrinkle and firming effects further comprises any one of a preservative, a pH regulator or a fragrance, or a combination of at least two of them.

9. A method for preparing an anti-wrinkle and firming essence according to any one of claims 5 to 8, characterized in that: The preparation method comprises: (1) Mixing a moisturizing agent, a thickener, an emollient, and water to obtain a mixed solution; (2) The mixed solution obtained in step (1), the composition with anti-wrinkle and firming effects, and an antioxidant are mixed and homogenized to obtain the essence with anti-wrinkle and firming effects.

10. The preparation method according to claim 9, characterized in that The mixing temperature in step (1) is 60-70°C, and the mixing time is 5-10 min; The mixing in step (1) further comprises adding a skin conditioning agent; The temperature of the mixing and homogenization in step (2) is 35-45°C, and the mixing time is 20-40 minutes; The step (2) of mixing and homogenizing further includes adding any one or a combination of at least two of a preservative, a pH adjuster or a fragrance.

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