Virus inactivation device for preparing artificial biological tendon

Abstract

The invention belongs to the technical field of virus inactivation equipment, and particularly relates to a virus inactivation device for artificial biological tendon preparation, which comprises a normal saline cylinder, the lower surface of the normal saline cylinder is fixedly connected with a plurality of support legs, and the outer walls of the plurality of support legs are jointly and fixedly connected with a bottom plate; the lower surface of the bottom plate is fixedly connected with a driving motor and a PLC. The virus inactivation device for preparing the artificial biological tendon has the functions of virus separation and inactivation, provenance cells and a biological scaffold material are prevented from being damaged in the virus inactivation process, the activity and the function of the provenance cells are guaranteed, and the adverse effect of the performance of the biological scaffold material is avoided; and the device has an efficient protection function through the heat preservation mechanism, so that the preparation quality of the artificial biological tendon is effectively improved, meanwhile, no accurate control step and no complex instrument are used in the virus inactivation process, time and labor are saved, and the preparation convenience and efficiency of the artificial biological tendon are effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of virus inactivation equipment, and in particular relates to a virus inactivation device for preparing artificial biological tendon. Background Art

[0002] Artificial bio-tendons are biomedical engineering products that simulate the structure and function of natural tendons and are used to replace damaged tendons. In the preparation of artificial bio-tendons, cell-derived materials and bio-scaffold materials need to be virus-inactivated. Seed-derived cells may carry viruses and be transmitted to patients. If the bio-scaffold materials are derived from animal tissues, they are easily contaminated by viruses, affecting the transplant effect. Virus inactivation can ensure the safety of artificial bio-tendons, prevent viral infection, protect human health after transplantation, improve product quality and reliability, and lay a safe foundation for clinical applications. For example, the patent with authorization announcement number CN212730501U discloses a virus inactivation device.

[0003] In the preparation of artificial bio-tendons, ensuring the safety of the seed cells and bio-scaffold materials is extremely critical. Traditional methods of viral inactivation currently have many drawbacks. For example, the peracetic acid-ethanol method, a commonly used chemical inactivation method, can achieve viral inactivation to a certain extent, but chemical reagents are very likely to remain. These residual reagents can adversely affect the activity and function of the seed cells, as well as the performance of the bio-scaffold materials, ultimately affecting the quality of the artificial bio-tendon. In the field of physical inactivation methods, gamma-ray irradiation can indeed effectively inactivate viruses. However, if the irradiation dose is not properly controlled, it will destroy the DNA structure of the germ cells, adversely affecting cell survival and the normal functioning of subsequent functions. Therefore, whether it is the amount of reagents used in the chemical inactivation method or the irradiation dose of gamma rays in the physical inactivation method, they must be precisely controlled according to the specific volume of the germ cells and biological scaffold materials. However, this precise control process is quite inconvenient. It not only requires professional technology and equipment, but also consumes a lot of time and energy, greatly reducing the convenience and efficiency of the virus inactivation link in the preparation of artificial biological tendons.

[0004] To this end, we proposed a virus inactivation device for artificial biological tendon preparation to solve the above problems. Summary of the Invention

[0005] The purpose of the present invention is to provide a virus inactivation device for preparing artificial biological tendon in response to the above problems.

[0006] To achieve the above-mentioned objectives, the present invention adopts the following technical solutions: a virus inactivation device for preparing artificial biological tendon, comprising a physiological saline cylinder, a plurality of support legs fixedly connected to the lower surface of the physiological saline cylinder, the outer walls of the plurality of support legs commonly fixedly connected to a bottom plate, a drive motor and a PLC controller fixedly connected to the lower surface of the bottom plate, a drive end of the drive motor passing through the upper surface of the bottom plate, a through hole formed at the bottom end of the physiological saline cylinder, a first sealed bearing fixedly connected to the hole wall of the through hole, an inner wall of the first sealed bearing fixedly connected to the drive end of the drive motor, a circular through hole formed on the upper surface of the physiological saline cylinder, a second sealed bearing fixedly connected to the hole wall of the circular through hole, a virus separation mechanism fixedly connected to the inner wall of the second sealed bearing; The inner wall of the physiological saline cylinder is fixedly connected with a virus inactivation mechanism; The side wall of the physiological saline cylinder is provided with a mounting hole, and the wall of the mounting hole is fixedly connected with a heat preservation mechanism; The inner wall of the physiological saline cylinder is fixedly connected with a physiological saline circulation mechanism.

[0007] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the virus separation mechanism includes a bio-scaffold filter cartridge fixedly connected to the inner wall of the second sealed bearing, the inner wall of the bio-scaffold filter cartridge is fixedly connected to two symmetrically distributed connecting rods, and the opposite sides of the two connecting rods are commonly fixedly connected to a first conduit, the top of the first conduit is fixedly connected to a cell filter cartridge, the top inner wall of the first conduit is fixedly connected to a first separation component, the bottom end of the bio-scaffold filter cartridge is fixedly connected to a second conduit, the top inner wall of the second conduit is fixedly connected to a second separation component, the bottom end of the second conduit is sealed and fixedly connected to the driving end of the drive motor, the bottom outer wall of the second conduit is provided with a drainage through hole, and the inner walls of the bio-scaffold filter cartridge and the cell filter cartridge are both fixedly connected to a silicone buffer sleeve.

[0008] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the virus inactivation mechanism includes a micro-pump fixedly connected to the inner wall of the bottom end of the physiological saline cylinder, the outer wall of the bottom end of the second catheter is fixedly sleeved with a third sealed bearing, the outer surface of the outer ring of the third sealed bearing is fixedly sleeved with an isolation cover, the bottom end of the isolation cover is fixedly connected to the inner wall of the bottom end of the physiological saline cylinder, the liquid inlet end of the micro-pump is fixedly connected to the outer wall of the isolation cover, the outlet end of the micro-pump is fixedly connected to a delivery pipe, the tube wall of the delivery pipe is fixedly sleeved with an arc cover, the outer wall of the arc cover is fixedly connected to the inner wall of the physiological saline cylinder, the upper surface of the physiological saline cylinder is provided with a fixed through hole, and the hole wall of the fixed through hole is fixedly connected with an electric heating tube, the electric heating tube is located inside the arc cover, and the outer wall of the bottom end of the arc cover is fixedly connected with a discharge one-way valve.

[0009] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the heat preservation mechanism includes a semiconductor refrigeration plate fixedly embedded in the side wall of the bottom end of the physiological saline cylinder, the cooling side of the semiconductor refrigeration plate is fixedly connected to a stainless steel heat-conducting mesh block, the heat dissipation side of the semiconductor refrigeration plate is fixedly connected to a heat sink, the outer wall of the heat sink is fixedly connected to a cooling fan, the bottom end of the physiological saline cylinder is provided with a fixing hole, and the hole wall of the fixing hole is fixedly connected to a temperature sensor.

[0010] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the physiological saline circulation mechanism includes a support plate fixedly connected to the inner wall of the bottom end of the physiological saline cylinder, the outer wall of the support plate is fixedly connected to a hollow cylinder, the bottom end of the hollow cylinder is provided with a through hole, and the hole wall of the through hole is fixedly connected to a T-shaped rod, the top end of the T-shaped rod is fixedly connected to a sealing cover, the outer wall of the sealing cover is sealingly and slidingly connected to the inner wall of the hollow cylinder, the rod wall of the T-shaped rod is sleeved with a reset spring, and the two ends of the reset spring are respectively connected to The outer wall of the T-shaped rod is fixedly connected to the inner wall of the hollow cylinder. The top of the hollow cylinder is fixedly connected to a liquid inlet one-way valve and a liquid outlet one-way valve. The liquid outlet end of the liquid outlet one-way valve is fixedly connected to an arc-shaped tube. The top of the arc-shaped tube passes through the upper surface of the physiological saline cylinder. The outlet end of the arc-shaped tube is located above the cell filtration cylinder. The tube wall of the arc-shaped tube is fixedly connected to an arc-shaped branch tube. The liquid outlet end of the arc-shaped branch tube is located above the biological scaffold filtration cylinder. The tube wall of the second conduit is fixedly sleeved with an inclined cylinder for raising and lowering the T-shaped rod.

[0011] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the first separation component includes a first clamping ring fixedly connected to the inner wall of the top end of the first catheter, the outer wall of the first clamping ring is clamped with a matching first silicone lip ring, and the inner wall of the first silicone lip ring is fixedly connected with a first virus separation membrane layer.

[0012] In the above-mentioned virus inactivation device for preparing artificial biological tendon, the second separation component includes a second clamping ring fixedly connected to the inner wall of the top end of the second catheter, the outer wall of the second clamping ring is clamped with a matching second silicone lip ring, and the inner wall of the second silicone lip ring is fixedly connected with a supporting mesh and a second virus separation membrane layer.

[0013] In the above-mentioned virus inactivation device for preparing artificial biological tendon, a physiological saline replenishing screw hole is opened at the top of the physiological saline cylinder, and the hole wall of the physiological saline replenishing screw hole is threadedly connected with a sealing plug, and a plurality of through holes are opened at the top of the physiological saline cylinder, and the hole walls of the plurality of through holes are fixedly connected with ultraviolet disinfection lamps of different wavelengths.

[0014] Compared with existing technologies, the advantages of a virus inactivation device for preparing artificial biological tendons are: 1. Through the virus separation mechanism and drive motor, when the seed-derived cells and bioscaffold materials need to be virus-inactivated before artificial bio-tendon preparation, physiological saline is first injected into the physiological saline cylinder, and the drive motor is started by the PLC controller. The drive motor rotates the cell filter cartridge and the bioscaffold filter cartridge in the virus separation mechanism. The cell filter cartridge generates centrifugal force during rotation. Since the mass of the seed-derived cells is relatively large, they are thrown to the edge of the cell filter cartridge under the action of centrifugal force. The mass of the viruses carried by the seed-derived cells is relatively small. Under the action of centrifugal force, the centripetal force required for the viruses to perform circular motion is also small. Because the viruses cannot obtain sufficient centripetal force, they are gradually separated from the seed-derived cells in the relative motion generated by the centrifugal force and the interaction with the surrounding medium, and are free to the top of the first tube. Similarly, the viruses on the bioscaffold material are also free to the top of the second tube under the action of centrifugal force and the centripetal force during the centrifugal process. This mechanism enables the virus inactivation device for artificial bio-tendon preparation to have the function of virus separation, so that the seed-derived cells and bioscaffold materials will not be damaged during virus inactivation, thereby ensuring the quality of artificial bio-tendon preparation.

[0015] 2. Through the virus inactivation mechanism and physiological saline circulation mechanism, during the virus separation process, the PLC controller simultaneously controls the micro-pump and the electric heating tube to be powered on and started. The electric heating tube generates heat and raises the temperature inside the arc cover to 85℃-93℃. This high temperature environment can be used to inactivate the physiological saline carrying viruses. The micro-pump of the virus inactivation mechanism can suck the physiological saline carrying viruses between the cell filter cartridge and the biological support filter cartridge. Finally, the viruses in the physiological saline are inactivated by high temperature in the arc cover. In addition, the driving motor controls the rotation of the first catheter and drives the physiological saline circulation mechanism to work. The physiological saline circulation machine The structure replenishes the physiological saline in the physiological saline cylinder into the cell filter cartridge and the bio-scaffold filter cartridge to ensure that there is sufficient virus-free physiological saline in the cell filter cartridge and the bio-scaffold filter cartridge to separate the viruses in the seed cells and the bio-scaffold materials. This cycle lasts for 15-20 minutes, which can effectively separate and inactivate the viruses carried by the seed cells and the bio-scaffold materials in the process of preparing artificial bio-tendon. This mechanism enables the virus inactivation device for artificial bio-tendon preparation to have the function of virus inactivation, and the virus inactivation process does not require precise control steps and the use of complex instruments, which saves time and effort, and effectively improves the convenience and efficiency of artificial bio-tendon preparation.

[0016] 3. Through the provided insulation mechanism, when the high-temperature saline discharged from the discharge one-way valve enters the saline cylinder, it is first mixed with the saline in the saline cylinder for preliminary cooling. At the same time, the insulation mechanism will detect the temperature of the saline. If the saline temperature exceeds the high-temperature threshold of 37°C preset by the PLC controller, the insulation mechanism will work to cool it down in time to avoid the high-temperature saline causing inactivation of the germ cells. When the temperature of the saline in the saline cylinder is lower than the high-temperature threshold of 37°C, the PLC controller will promptly control the semiconductor refrigeration plate and the cooling fan to pause to avoid the continuous cooling of the semiconductor refrigeration plate causing the saline temperature to be too low to damage the germ cells, thereby ensuring the safety of the germ cells. This mechanism enables the virus inactivation device for artificial biological tendon preparation to have the function of germ cell safety protection, thereby improving the reliability of equipment use. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic structural diagram of a virus inactivation device for preparing artificial biological tendon provided by the present invention; Figure 2 This is a schematic cross-sectional structural diagram of a virus inactivation device for preparing an artificial biological tendon provided by the present invention; Figure 3 yes Figure 2 A schematic diagram of the partially enlarged structure; Figure 4 This is a schematic structural diagram of a physiological saline circulation mechanism in a virus inactivation device for preparing an artificial biological tendon provided by the present invention; Figure 5 This is a schematic structural diagram of a heat sink in a virus inactivation device for preparing an artificial biological tendon provided by the present invention; Figure 6 This is a schematic structural diagram of an inclined cylinder in a virus inactivation device for preparing an artificial biological tendon provided by the present invention; Figure 7 This is a schematic structural diagram of the first separation component in a virus inactivation device for preparing artificial biological tendon provided by the present invention; Figure 8 It is a structural schematic diagram of the second separation component in a virus inactivation device for preparing artificial biological tendon provided by the present invention.

[0018] In the figure: 1 physiological saline cylinder, 2 support legs, 3 bottom plate, 4 virus separation mechanism, 41 biological support filter cartridge, 42 connecting rod, 43 first conduit, 44 cell filter cartridge, 45 second conduit, 46 drainage through hole, 47 silicone cushion sleeve, 5 virus inactivation mechanism, 51 micro-pump, 52 third sealed bearing, 53 isolation cover, 54 delivery pipe, 55 arc cover, 56 electric heating pipe, 57 discharge check valve, 6 insulation mechanism, 61 semiconductor cooling plate, 62 stainless steel heat conductive mesh block, 63 heat sink, 64 cooling fan, 65 temperature sensor, 7 physiological saline circulation machine Structure, 71 support plate, 72 hollow cylinder, 73 T-shaped rod, 74 sealing cover, 75 return spring, 76 liquid inlet check valve, 77 liquid outlet check valve, 78 arc tube, 79 arc branch pipe, 710 inclined cylinder, 8 first separation component, 81 first clamping ring, 82 first silicone lip ring, 83 first virus separation membrane layer, 9 second separation component, 91 second clamping ring, 92 second silicone lip ring, 93 support mesh, 94 second virus separation membrane layer, 10 PLC controller, 11 first sealed bearing, 12 second sealed bearing, 13 drive motor, 14 sealing plug, 15 ultraviolet disinfection lamp. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] like Figures 1-8As shown, a virus inactivation device for preparing artificial biological tendon includes a physiological saline cylinder 1, a plurality of support legs 2 are fixedly connected to the lower surface of the physiological saline cylinder 1, the outer walls of the plurality of support legs 2 are fixedly connected to a bottom plate 3, a drive motor 13 and a PLC controller 10 are fixedly connected to the lower surface of the bottom plate 3, the driving end of the drive motor 13 passes through the upper surface of the bottom plate 3, a through hole is provided at the bottom end of the physiological saline cylinder 1, and a first sealed bearing 11 is fixedly connected to the hole wall of the through hole, the inner wall of the first sealed bearing 11 is fixedly connected to the driving end of the drive motor 13, a circular through hole is provided on the upper surface of the physiological saline cylinder 1, and a second sealed bearing 12 is fixedly connected to the hole wall of the circular through hole, the inner wall of the second sealed bearing 12 is fixedly connected to a virus separation mechanism 4, and the virus separation mechanism 4 includes a biological support filter cartridge 41 fixedly connected to the inner wall of the second sealed bearing 12 The inner wall of the bio-scaffold filter cartridge 41 is fixedly connected to two symmetrically distributed connecting rods 42, and the opposite sides of the two connecting rods 42 are commonly fixedly connected to a first conduit 43, the top of the first conduit 43 is fixedly connected to a cell filter cartridge 44, the top inner wall of the first conduit 43 is fixedly connected to a first separation component 8, the bottom end of the bio-scaffold filter cartridge 41 is fixedly connected to a second conduit 45, the top inner wall of the second conduit 45 is fixedly connected to a second separation component 9, the bottom end of the second conduit 45 is sealed and fixedly connected to the driving end of the drive motor 13, and a drainage hole 46 is provided on the outer wall of the bottom end of the second conduit 45. The inner walls of the bio-scaffold filter cartridge 41 and the cell filter cartridge 44 are fixedly connected to a silicone buffer sleeve 47. This mechanism can separate the virus and then inactivate it without affecting the raw materials for preparing artificial biological tendons, thereby improving the success rate and effect of artificial biological tendon preparation.

[0021] The first separation component 8 includes a first clamping ring 81 fixedly connected to the inner wall of the top end of the first catheter 43, the outer wall of the first clamping ring 81 is clamped with a matching first silicone lip ring 82, and the inner wall of the first silicone lip ring 82 is fixedly connected to the first virus separation membrane layer 83. The second separation component 9 includes a second clamping ring 91 fixedly connected to the inner wall of the top end of the second catheter 45, the outer wall of the second clamping ring 91 is clamped with a matching second silicone lip ring 92, and the inner wall of the second silicone lip ring 92 is fixedly connected to a supporting mesh 93 and a second virus separation membrane layer 94. Both the first virus separation membrane layer 83 and the second virus separation membrane layer 94 can allow viruses and saline to pass through, thereby ensuring the effect of virus separation.

[0022] The inner wall of the physiological saline cylinder 1 is fixedly connected to a virus inactivation mechanism 5, which includes a micro-liquid pump 51 fixedly connected to the inner wall of the bottom end of the physiological saline cylinder 1. The outer wall of the bottom end of the second conduit 45 is fixedly sleeved with a third sealed bearing 52. The outer surface of the outer ring of the third sealed bearing 52 is fixedly sleeved with an isolation cover 53. The bottom end of the isolation cover 53 is fixedly connected to the inner wall of the bottom end of the physiological saline cylinder 1. The liquid inlet end of the micro-liquid pump 51 is fixedly connected to the outer wall of the isolation cover 53. The outlet end of the micro-liquid pump 51 is fixedly connected to the outer wall of the isolation cover 53. There is a delivery pipe 54, and the wall of the delivery pipe 54 is fixedly sleeved with an arc cover 55. The outer wall of the arc cover 55 is fixedly connected to the inner wall of the saline cylinder 1. A fixed through hole is opened on the upper surface of the saline cylinder 1, and the hole wall of the fixed through hole is fixedly connected with an electric heating pipe 56. The electric heating pipe 56 is located inside the arc cover 55. The outer wall of the bottom end of the arc cover 55 is fixedly connected to a discharge one-way valve 57. This mechanism can effectively inactivate viruses in raw materials during the preparation of artificial biological tendons and ensure the quality of artificial biological tendon preparation.

[0023] A mounting hole is provided on the side wall of the physiological saline cylinder 1, and a heat preservation mechanism 6 is fixedly connected to the hole wall of the mounting hole. The heat preservation mechanism 6 includes a semiconductor refrigeration plate 61 fixedly embedded in the side wall of the bottom end of the physiological saline cylinder 1, and the cooling side of the semiconductor refrigeration plate 61 is fixedly connected to a stainless steel heat-conducting mesh block 62, and the heat dissipation side of the semiconductor refrigeration plate 61 is fixedly connected to a heat sink 63. The outer wall of the heat sink 63 is fixedly connected to a heat dissipation fan 64. A fixing hole is provided at the bottom end of the physiological saline cylinder 1, and a temperature sensor 65 is fixedly connected to the hole wall of the fixing hole. This mechanism enables the virus inactivation device for preparing artificial biological tendon to have the function of seed-derived cell safety protection, thereby improving the reliability of the equipment.

[0024] The inner wall of the physiological saline cylinder 1 is fixedly connected to a physiological saline circulation mechanism 7, which includes a support plate 71 fixedly connected to the inner wall of the bottom end of the physiological saline cylinder 1, and the outer wall of the support plate 71 is fixedly connected to a hollow cylinder 72. The bottom end of the hollow cylinder 72 is provided with a through hole, and the hole wall of the through hole is fixedly connected to a T-shaped rod 73. The top of the T-shaped rod 73 is fixedly connected to a sealing cover 74. The outer wall of the sealing cover 74 is sealingly and slidingly connected to the inner wall of the hollow cylinder 72. The rod wall of the T-shaped rod 73 is sleeved with a reset spring 75. The two ends of the reset spring 75 are fixedly connected to the outer wall of the T-shaped rod 73 and the inner wall of the hollow cylinder 72 respectively. The top of the hollow cylinder 72 is fixedly connected to a liquid inlet check valve 76 and a liquid outlet check valve 77. The liquid outlet end of the liquid outlet check valve 77 is fixedly connected to an arc tube 78. The top of the arc tube 78 passes through the upper surface of the physiological saline cylinder 1. The outlet end of the arc tube 78 is located above the cell filter cartridge 44. The wall of the arc tube 78 is fixedly connected to an arc branch pipe 79. The liquid outlet end of the arc branch pipe 79 is located above the bio-scaffold filter cartridge 41. The wall of the second conduit 45 is fixedly connected to an inclined cylinder 710 for raising and lowering the T-shaped rod 73. This mechanism can realize the recycling of physiological saline, thereby achieving the purpose of saving physiological saline.

[0025] A physiological saline replenishing screw hole is provided at the top of the physiological saline cylinder 1, and a sealing plug 14 is threadedly connected to the hole wall of the physiological saline replenishing screw hole. A plurality of through holes are provided at the top of the physiological saline cylinder 1, and ultraviolet disinfection lamps 15 with different wavelengths are fixedly connected to the hole walls of the plurality of through holes. The ultraviolet disinfection lamps 15 with different wavelengths have the function of efficiently inactivating certain types of viruses. For example, the light of a 254nm wavelength ultraviolet lamp can efficiently inactivate a variety of viruses including influenza virus, hepatitis B virus, bacteriophage virus, polio virus, etc., and the light of a 222nm wavelength ultraviolet lamp has a significant inactivation effect on a variety of viruses such as H1N1 influenza virus. The virus inactivation effect of the equipment is assisted by multiple ultraviolet disinfection lamps 15.

[0026] The micro liquid pump 51, electric heating tube 56, semiconductor refrigeration plate 61, cooling fan 64, drive motor 13 and ultraviolet disinfection lamp 15 are all electrically connected to the output end of the PLC controller 10 through wires, and the temperature sensor 65 is electrically connected to the input end of the PLC controller 10 through wires. The above-mentioned power-on equipment and electrical connections are all existing technologies and will not be repeated here.

[0027] The operating principle of the present invention is described as follows: when it is necessary to inactivate viruses of the seed-derived cells and the bioscaffold material before preparing the artificial biological tendon, first open the sealing plug 14, and inject physiological saline into the physiological saline cylinder 1 until the physiological saline cylinder 1 is filled. After filling, install the sealing plug 14, and at the same time, inject an appropriate amount of physiological saline into the cell filter cylinder 44 and the bioscaffold filter cylinder 41. Then, the seed-derived cells are placed in the cell filter cylinder 44, and the bioscaffold material is placed in the bioscaffold filter cylinder 41. Then, the PLC controller 10 controls the start of the drive motor 13 and controls the speed of the drive motor 13 to the safe speed R1 (at the safe speed R1, the seed-derived cells will not be damaged when centrifuged, which can ensure the biological activity of the seed-derived cells). Then, the drive motor 13 drives the second catheter 45 to The bio-scaffold filter cartridge 41 rotates, and the bio-scaffold filter cartridge 41 causes the cell filter cartridge 44 to rotate together through the connecting rod 42. The rotation of the cell filter cartridge 44 generates centrifugal force. Since the mass of the seed-derived cells is relatively large, they will be thrown to the edge of the cell filter cartridge 44 under the action of the centrifugal force. The mass of the viruses carried by the seed-derived cells is relatively small. Under the action of the centrifugal force, the centripetal force required for the viruses to perform circular motion is also small. The seed-derived cells provide a large centripetal force at the edge to maintain the circular motion. The viruses, unable to obtain sufficient centripetal force, gradually separate from the seed-derived cells in the relative motion generated by the centrifugal force and the interaction with the surrounding medium, and float to the top of the first conduit 43. Similarly, the viruses on the bio-scaffold material will also float to the top of the second conduit 45 under the action of the centrifugal force and the centripetal force during the centrifugal process. At the same time, the PLC controller 10 simultaneously controls the micro-liquid pump 51 and the electric heating tube 56 to be powered on and started. The electric heating tube 56 generates heat, raising the temperature inside the arc cover 55 to 85°C-93°C. This high temperature environment can be used to inactivate the physiological saline carrying the virus. The micro-liquid pump 51 sucks the air and physiological saline inside the isolation cover 53, so that the isolation cover 53 is in a negative pressure state. The isolation cover 53 in a negative pressure state sucks the air and physiological saline in the second conduit 45 through the drainage hole 46, thereby making the second conduit 45 also in a negative pressure state. Then the second conduit 45 in a negative pressure state sucks the physiological saline at the center of the bio-scaffold filter cartridge 41 through the second virus separation membrane layer 94. At this time, the physiological saline carrying the virus at the center of the bio-scaffold filter cartridge 41 passes through the second virus separation membrane layer 94. Since the filtration channel of the second virus separation membrane layer 94 can meet the passage of the virus, the virus also passes through the second virus separation membrane layer 94 with the physiological saline, and due to the physiological saline in the bio-scaffold filter cartridge 41 The pressure inside the first conduit 43 is reduced, and the bottom of the first conduit 43 is submerged in the physiological saline inside the bio-scaffold filter cartridge 41, so that the interior of the first conduit 43 is also in a negative pressure state. The first conduit 43 in the negative pressure state sucks the physiological saline at the center of the cell filter cartridge 44 through the first virus separation membrane layer 83. At this time, the physiological saline carrying the virus at the center of the cell filter cartridge 44 passes through the first virus separation membrane layer 83. Since the filtration channel of the first virus separation membrane layer 83 can meet the requirements of virus penetration, the virus also passes through the first virus separation membrane layer 83 along with the physiological saline. Then, the physiological saline carrying the virus flows to the center of the bio-scaffold filter cartridge 41 and passes through the second virus separation membrane layer 94 along with the physiological saline at the center of the bio-scaffold filter cartridge 41. Finally, the physiological saline carrying the virus is transported to the inside of the arc cover 55 through the micro-liquid pump 51 and the delivery pipe 54, and the high temperature environment inside the arc cover 55 is used to achieve the purpose of inactivating the virus. The physiological saline after virus inactivation then flows back into the physiological saline cylinder 1 through the discharge one-way valve 57; When the first tube 43 is driven by the driving motor 13 to rotate, the inclined cylinder 710 is also driven to rotate. The protruding portion of the inclined cylinder 710 will push the T-shaped rod 73 upward. While the T-shaped rod 73 pushes the sealing cover 74 to move upward, it also drives the return spring 75 to stretch, so that the return spring 75 has a rebound force. After the protruding portion of the inclined cylinder 710 is away from the T-shaped rod 73, the T-shaped rod 73 moves downward under the action of the rebound force of the return spring 75, and the bottom of the T-shaped rod 73 contacts the bottom of the inclined cylinder 710. In this way, the inclined cylinder 710 rotates one circle to make the T-shaped rod 73 reciprocate up and down once. When the T-shaped rod 73 drives the sealing cover 74 to move downward, the physiological saline in the physiological saline cylinder 1 is affected by the negative pressure inside the hollow cylinder 72 and enters the interior of the hollow cylinder 72 through the liquid inlet check valve 76; when the T-shaped rod 73 drives the sealing cover 74 to move upward, the physiological saline in the hollow cylinder 72 passes through the liquid outlet check valve 77 and enters the arc tube 78. The physiological saline in the arc tube 78 passes through The top outlet of the arc tube 78 and the outlet of the arc branch 79 are respectively supplied to the cell filter cartridge 44 and the bioscaffold filter cartridge 41, ensuring that there is sufficient virus-free physiological saline in the cell filter cartridge 44 and the bioscaffold filter cartridge 41 to separate the viruses in the seed-derived cells and the bioscaffold material. This cycle is repeated for 15-20 minutes, which can effectively separate and inactivate the viruses carried by the seed-derived cells and the bioscaffold material in the process of preparing the artificial bio-tendon. This mechanism enables the virus inactivation device for preparing the artificial bio-tendon to have the functions of virus separation and inactivation, thereby preventing the seed-derived cells and the bioscaffold material from being damaged during the virus inactivation process, ensuring the activity and function of the seed-derived cells, and avoiding adverse effects on the performance of the bioscaffold material, thereby effectively improving the quality of the artificial bio-tendon preparation. In addition, the virus inactivation process does not require precise control steps and the use of complex instruments, saving time and effort, and effectively improving the convenience and efficiency of the artificial bio-tendon preparation. When the high-temperature saline discharged from the discharge one-way valve 57 enters the saline cylinder 1, it is first mixed with the saline in the saline cylinder 1 to be initially cooled. In addition, the temperature sensor 65 also detects the temperature of the saline in the saline cylinder 1 in real time and sends the temperature value to the PLC controller 10 in the form of an electrical signal. If the temperature of the saline in the saline cylinder 1 exceeds the high-temperature threshold of 37°C preset by the PLC controller 10, the PLC controller 10 receives the electrical signal from the temperature sensor 65 and promptly controls the semiconductor refrigeration plate 61 and the cooling fan 64 to start. The cooling side of the semiconductor refrigeration plate 61 promptly reduces the temperature of the saline through the stainless steel heat-conducting mesh block 62 to avoid the inactivation of the seed-derived cells caused by the high-temperature saline. At the same time, the cooling fan The fan 64 can speed up the heat dissipation speed of the heat sink 63, so that the heat dissipation side of the semiconductor refrigeration plate 61 can dissipate heat efficiently, thereby ensuring the cooling effect of the semiconductor refrigeration plate 61. After the temperature sensor 65 detects that the temperature of the physiological saline in the physiological saline cylinder 1 is lower than the high temperature threshold of 37°C, the PLC controller 10 promptly controls the semiconductor refrigeration plate 61 and the heat dissipation fan 64 to pause, so as to avoid the continuous cooling of the semiconductor refrigeration plate 61 causing the physiological saline temperature to be too low to damage the seed cells, thereby ensuring the safety of the seed cells. After the virus of the seed cells and the biological scaffold material is inactivated, they are placed in a professional artificial biological tendon preparation equipment for preparation. This mechanism enables the virus inactivation device for artificial biological tendon preparation to have the function of seed cell safety protection, thereby improving the reliability of the equipment.

[0028] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A virus inactivation device for preparing artificial biological tendon, comprising a normal saline cylinder (1), characterized in that: The lower surface of the physiological saline cylinder (1) is fixedly connected to a plurality of support legs (2), the outer walls of the plurality of support legs (2) are fixedly connected to a bottom plate (3), the lower surface of the bottom plate (3) is fixedly connected to a drive motor (13) and a PLC controller (10), the drive end of the drive motor (13) passes through the upper surface of the bottom plate (3), a through hole is provided at the bottom end of the physiological saline cylinder (1), and a first sealed bearing (11) is fixedly connected to the hole wall of the through hole, the inner wall of the first sealed bearing (11) is fixedly connected to the drive end of the drive motor (13), a circular through hole is provided on the upper surface of the physiological saline cylinder (1), and a second sealed bearing (12) is fixedly connected to the hole wall of the circular through hole, and the inner wall of the second sealed bearing (12) is fixedly connected to the virus separation mechanism (4); A virus inactivation mechanism (5) is fixedly connected to the inner wall of the physiological saline cylinder (1); A mounting hole is provided on the side wall of the physiological saline cylinder (1), and a heat preservation mechanism (6) is fixedly connected to the wall of the mounting hole; A physiological saline circulation mechanism (7) is fixedly connected to the inner wall of the physiological saline cylinder (1).

2. A virus inactivation device for preparing artificial biological tendon according to claim 1, characterized in that: The virus separation mechanism (4) comprises a bio-scaffold filter cartridge (41) fixedly connected to the inner wall of the second sealed bearing (12); the inner wall of the bio-scaffold filter cartridge (41) is fixedly connected to two symmetrically distributed connecting rods (42); opposite sides of the two connecting rods (42) are fixedly connected to a first conduit (43); the top end of the first conduit (43) is fixedly connected to a cell filter cartridge (44); the top inner wall of the first conduit (43) is fixedly connected to a first separation component (8); the bottom end of the bio-scaffold filter cartridge (41) is fixedly connected to a second conduit (45); the top inner wall of the second conduit (45) is fixedly connected to a second separation component (9); the bottom end of the second conduit (45) is sealed and fixedly connected to the driving end of the driving motor (13); the bottom outer wall of the second conduit (45) is provided with a drainage through hole (46); the inner walls of the bio-scaffold filter cartridge (41) and the cell filter cartridge (44) are both fixedly connected to a silicone buffer sleeve (47).

3. A virus inactivation device for preparing artificial biological tendon according to claim 2, characterized in that: The virus inactivation mechanism (5) comprises a micro-liquid pump (51) fixedly connected to the inner wall of the bottom end of the physiological saline cylinder (1); the outer wall of the bottom end of the second conduit (45) is fixedly sleeved with a third sealing bearing (52); the outer surface of the outer ring of the third sealing bearing (52) is fixedly sleeved with an isolation cover (53); the bottom end of the isolation cover (53) is fixedly connected to the inner wall of the bottom end of the physiological saline cylinder (1); the liquid inlet end of the micro-liquid pump (51) is fixedly connected to the outer wall of the isolation cover (53); the micro-liquid pump The outlet end of (51) is fixedly connected to a delivery pipe (54), the wall of the delivery pipe (54) is fixedly sleeved with an arc cover (55), the outer wall of the arc cover (55) is fixedly connected to the inner wall of the physiological saline cylinder (1), the upper surface of the physiological saline cylinder (1) is provided with a fixed through hole, and the hole wall of the fixed through hole is fixedly connected to an electric heating pipe (56), the electric heating pipe (56) is located inside the arc cover (55), and the outer wall of the bottom end of the arc cover (55) is fixedly connected to a discharge one-way valve (57).

4. A virus inactivation device for preparing artificial biological tendon according to claim 1, characterized in that: The heat preservation mechanism (6) comprises a semiconductor refrigeration plate (61) fixedly embedded in the side wall of the bottom end of the physiological saline cylinder (1); the cooling side of the semiconductor refrigeration plate (61) is fixedly connected to a stainless steel heat-conducting mesh block (62); the heat dissipation side of the semiconductor refrigeration plate (61) is fixedly connected to a heat sink (63); the outer wall of the heat sink (63) is fixedly connected to a heat dissipation fan (64); the bottom end of the physiological saline cylinder (1) is provided with a fixing hole, and the hole wall of the fixing hole is fixedly connected to a temperature sensor (65).

5. The virus inactivation device for preparing artificial biological tendon according to claim 2, characterized in that: The physiological saline circulation mechanism (7) comprises a support plate (71) fixedly connected to the inner wall of the bottom end of the physiological saline cylinder (1); the outer wall of the support plate (71) is fixedly connected to a hollow cylinder (72); the bottom end of the hollow cylinder (72) is provided with a through hole, and the hole wall of the through hole is fixedly connected to a T-shaped rod (73); the top end of the T-shaped rod (73) is fixedly connected to a sealing cover (74); the outer wall of the sealing cover (74) is sealingly and slidingly connected to the inner wall of the hollow cylinder (72); the rod wall of the T-shaped rod (73) is sleeved with a reset spring (75); the two ends of the reset spring (75) are respectively fixed to the outer wall of the T-shaped rod (73) and the inner wall of the hollow cylinder (72). The top end of the hollow tube (72) is fixedly connected to a liquid inlet check valve (76) and a liquid outlet check valve (77), the liquid outlet end of the liquid outlet check valve (77) is fixedly connected to an arc tube (78), the top end of the arc tube (78) passes through the upper surface of the physiological saline cylinder (1), the outlet end of the arc tube (78) is located above the cell filter cylinder (44), the wall of the arc tube (78) is fixedly connected to an arc branch tube (79), the liquid outlet end of the arc branch tube (79) is located above the biological support filter cylinder (41), and the wall of the second conduit (45) is fixedly sleeved with an inclined tube (710) for lifting and lowering the T-shaped rod (73).

6. A virus inactivation device for preparing artificial biological tendon according to claim 2, characterized in that: The first separation component (8) includes a first clamping ring (81) fixedly connected to the inner wall of the top end of the first conduit (43), the outer wall of the first clamping ring (81) is clamped with a matching first silicone lip ring (82), and the inner wall of the first silicone lip ring (82) is fixedly connected with a first virus separation membrane layer (83).

7. A virus inactivation device for preparing artificial biological tendon according to claim 2, characterized in that: The second separation component (9) includes a second clamping ring (91) fixedly connected to the inner wall of the top end of the second conduit (45), the outer wall of the second clamping ring (91) is clamped with a matching second silicone lip ring (92), and the inner wall of the second silicone lip ring (92) is fixedly connected with a supporting mesh (93) and a second virus separation membrane layer (94).

8. The virus inactivation device for preparing artificial biological tendon according to claim 1, characterized in that: The top of the physiological saline cylinder (1) is provided with a physiological saline replenishing screw hole, and the hole wall of the physiological saline replenishing screw hole is threadedly connected to a sealing plug (14). The top of the physiological saline cylinder (1) is provided with a plurality of through holes, and the hole walls of the plurality of through holes are fixedly connected to ultraviolet disinfection lamps (15) of different wavelengths.

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