Special biological bacterial fertilizer for Citrus chachiensis and application thereof

Abstract

The invention discloses a special biological bacterial fertilizer for Citrus chachiensis and application thereof. The special biological bacterial fertilizer for Citrus chachiensis comprises Streptomyces malachite and a basic fertilizer, and the Streptomyces malachite and the basic fertilizer containing nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium are mixed for use, so that the Streptomyces malachite and the fertilizer are combined for use to achieve a remarkable synergistic effect; when the malachite streptomyces spinosus or the fertilizer is singly used, the growth index of the Citrus chachiensis and the improvement degree of the content of effective components of the Citrus chachiensis are lower than those of the combined use of the two. After the two components are combined for use, the growth of the Citrus chachiensis can be remarkably promoted, the content of effective components of the Citrus chachiensis can be remarkably increased, and the increasing rate reaches 49.79%-78.35%; the combined use also reduces the use amounts of the two, the effect is significantly improved, and the prepared biological bacterial fertilizer is used in the practice operation of artificial planting of Citrus chachiensis, and creates conditions for increasing the volatile oil content of Citrus chachiensis and expanding the planting scale.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial fertilizers, and more specifically relates to a special biological fertilizer for tea-branch oranges and its application. Background Art

[0002] Guang Chenpi (Chinese tangerine peel) is the outer peel of the tea-branched citrus plant (Citrus sinensis), a member of the Rutaceae family. After aging for 2-3 years, it can be used as medicine. Currently, Guang Chenpi is used in a variety of applications, including food, pharmaceuticals, health supplements, and daily chemical products, earning it the reputation of "one ounce of tangerine peel is worth one ounce of gold." Tea-branched citrus is the source plant for Guang Chenpi. Because tea-branched citrus is primarily grown in farmland and other cultivated land, this has reduced my country's arable land area. With the demands of national development, Guang Chenpi cultivation urgently needs to shift from fields to mountainous forests. During the industrial transfer from Guang Chenpi's authentic production areas, changes in the soil environment can lead to a decline in Guang Chenpi's quality, and the application of conventional fertilizers is insufficient to improve its quality. Therefore, there is an urgent need to address the issue of Guang Chenpi's quality degradation caused by the shift in tea-branched citrus cultivation and the resulting changes in the soil environment.

[0003] The growth and quality improvement of tea-branch oranges involve multiple factors, including soil management, the application of microbial fertilizers, and cultivation techniques. Currently, a variety of microbial strains and fertilizers have been studied or applied in tea-branch orange cultivation, but some technical bottlenecks and shortcomings remain. Existing research suggests that microbial strains / fertilizers that can promote the growth and quality of tea-branch oranges include: nitrogen-fixing bacteria (such as Azospirillum brasilense): They promote nitrogen absorption and reduce reliance on chemical fertilizers. Phosphate-solubilizing bacteria (such as Bacillus megaterium): They release insoluble phosphorus from the soil, enhancing sugar accumulation in the fruit. Potassium-solubilizing bacteria (such as Bacillus mucilaginosus): They activate potassium and improve fruit flavor. Plant growth-promoting rhizobacteria (PGPR) (such as Pseudomonas spp.): They secrete growth hormones (IAA) to stimulate root development. Commonly used commercial fertilizers include: compound microbial fertilizers (containing multiple functional bacteria), such as EM bacteria (effective microbial communities), and rhizobium agents. And specific functional fertilizers: such as Bacillus amyloliquefaciens for the prevention and control of Huanglongbing

[0004] Similarly, the above-mentioned microbial strains / fertilizers also have certain problems and defects, such as poor strain adaptability: due to the susceptibility of the soil environment, the colonization of exogenous strains may fail, and the effect is unstable. Conflicts in the compatibility of compound microbial agents: There may be antagonistic effects between different strains (such as some PGPRs inhibiting fungal growth), and the compounding ratio needs to be optimized. Limited long-term effects: dependence on a single microbial fertilizer may lead to an imbalance in the soil microecology, and it needs to be used in combination with organic fertilizers (such as compost and green manure). The mechanism of quality improvement is unclear: how microorganisms affect the synthesis pathways of key quality components of tea citrus (such as volatile oils and hesperidin) still requires molecular research.

[0005] Therefore, there is still a lack of more efficient, stable and adaptable microbial products that can better solve the problem of quality decline during industrial transfer in the authentic production areas of Guang Chenpi, and the inability of ordinary fertilizers to increase the content of effective ingredients in Guang Chenpi. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the defects and shortcomings of the existing Guang Chenpi, such as the quality decline during the industrial transfer of the authentic production area and the inability of ordinary fertilizers to increase the content of effective ingredients in Guang Chenpi, and to provide a special biological fertilizer for tea-branch oranges and its application.

[0007] The invention aims to provide a special biological fertilizer for tea citrus.

[0008] Another object of the present invention is to provide an application of a special biological fertilizer for tea citrus.

[0009] Another object of the present invention is to provide an application of Streptomyces malachite spinulosa in combination with fertilizer in tea-branch citrus.

[0010] Another object of the present invention is to provide a method for promoting the growth of tea-branch citrus or increasing the content of effective ingredients in tea-branch citrus.

[0011] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0012] The invention provides a special biological fertilizer for tea citrus. The biological fertilizer comprises Streptomyces malachite and a basic fertilizer. The volume ratio of the Streptomyces malachite to the basic fertilizer is 1:(1-2); the mass ratio of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium nutrient elements contained in the basic fertilizer is (135-140):(20-25):(55-60):1:(1-5):(1-5):(155-160); and the OD value of the Streptomyces malachite has a value of 0.6-0.8.

[0013] The present invention combines Streptomyces malachite with a basic fertilizer containing nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, and sodium nutrients. The invention finds that the combination of Streptomyces malachite and the basic fertilizer has a significant synergistic effect. When Streptomyces malachite or the fertilizer is used alone, the two have a similar effect on the growth of tea-branch citrus, both promoting the growth of tea-branch citrus and increasing the content of the main monoterpenes (α-pinene, α-thujene, β-pinene, and β-myrcene), the active ingredients of tea-branch citrus. When the basic fertilizer is used alone, the increase rates of plant height, chlorophyll a, chlorophyll b, and total chlorophyll are 26.66%, 12.53%, 20.39%, and 16.15%, respectively. The increase rates of α-pinene, α-thujene, β-pinene, and β-myrcene are 56.71%, 31.70%, 37.75%, and 26.34%, respectively. When the malachite-spined Streptomyces liquid was applied alone, the increase rates of plant height, chlorophyll a, chlorophyll b and total chlorophyll were 28.23%, 14.92%, 11.05% and 18.70% respectively, and the increase rates of α-pinene, α-thujacene, β-pinene and β-myrcene were 67.53%, 38.80%, 42.62% and 31.69% respectively. The combined use of Streptomyces malachite and basic fertilizer significantly promoted the growth of tea-branch oranges and increased their active ingredient content. The combined use of Streptomyces malachite and basic fertilizer increased plant height, chlorophyll a, chlorophyll b, and total chlorophyll by 57.24%, 25.74%, 27.53%, and 26.57%, respectively. The increases in α-pinene, α-thujane, β-pinene, and β-myrcene were 78.35%, 51.22%, 53.92%, and 49.79%, respectively. The combined use reduced the dosage of both fertilizers, achieving significant improvements. The growth indicators of tea-branch oranges were significantly enhanced, and the active ingredient content in dried tangerine peel was effectively increased, demonstrating that Streptomyces malachite can be used as a fertilizer synergist. Therefore, the combination of Streptomyces pomatia and fertilizer promoted the growth and development of tea-branch orange plants, increased the content of effective ingredients in tea-branch oranges, and can further improve the quality of tea-branch oranges. The prepared biological fertilizer was used in the practical operation of artificial cultivation of Guang Chenpi, creating conditions for increasing the volatile oil content of Guang Chenpi and expanding the scale of cultivation.

[0014] More preferably, the mass ratio of the nutrient elements nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium contained in the basic fertilizer is 138:22:57:1:3:4:157.

[0015] Preferably, the Streptomyces malachite is a Streptomyces malachite bacterial liquid, and the basic fertilizer is a nutrient solution.

[0016] More preferably, the bacterial liquid of Streptomyces malachite is obtained by culturing Streptomyces malachite in a liquid culture medium; the components of the liquid culture medium are 10 g / L tryptone, 5 g / L yeast extract, and 10 g / L NaCl.

[0017] More preferably, the nutrient solution comprises 1-3 g / L of potassium dihydrogen phosphate, 0.5-1 g / L of ammonium citrate, 0.01-0.05 g / L of calcium chloride, 0.1-0.3 g / L of magnesium sulfate, and 1-3 g / L of sodium chloride.

[0018] In particular, in this field, the combination of nutrient solution (liquid fertilizer) + bacterial solution is one of the preferred options. The combination of Streptomyces malachite and basic fertilizer can also be achieved by using a basic fertilizer (solid fertilizer) containing a Streptomyces malachite agent (such as a freeze-dried powder) and nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, and sodium nutrient elements.

[0019] More preferably, the concentration of the bacterial solution is not less than 5×10 11 cfu / L.

[0020] More preferably, the nutrient solution comprises 2.0 g / L potassium dihydrogen phosphate, 0.8 g / L ammonium citrate, 0.03 g / L calcium chloride, 0.16 g / L magnesium sulfate, and 2 g / L sodium chloride.

[0021] Preferably, the Streptomyces sp. is the Streptomyces sp. Strep-4 strain, which was deposited in the Guangdong Provincial Microbiological Culture Collection Center on September 30, 2022, with the culture collection number GDMCC NO: 62847.

[0022] As a preferred embodiment, the present invention also provides a method for preparing the above-mentioned biological fertilizer: culturing Streptomyces malachite spinulosa to a bacterial solution OD 600 The concentration of the supernatant was 0.8. After centrifugation, the supernatant was removed and an equal volume of sterile water was added. Accurately weigh potassium dihydrogen phosphate, ammonium citrate, calcium chloride, magnesium sulfate, and sodium chloride and fully dissolve them in purified water. The mixture was then mixed in a 1:1 volume ratio of bacterial suspension to nutrient solution.

[0023] The invention provides application of biological bacterial fertilizer in promoting the growth of tea-branch orange or increasing the content of effective components of tea-branch orange.

[0024] The invention provides an application of Streptomyces malachite spinulosa in combination with a basic fertilizer in promoting the growth of tea-branch citrus or increasing the content of effective components in tea-branch citrus.

[0025] The present invention provides an application of Streptomyces malachite spinulosa in combination with a basic fertilizer in preparing a product for promoting the growth of tea-branch citrus or increasing the content of effective ingredients in tea-branch citrus.

[0026] The present invention provides the use of Streptomyces malachite spinulosa as a fertilizer synergist in promoting the growth of tea-branch citrus or increasing the content of effective ingredients in tea-branch citrus.

[0027] Furthermore, the effective ingredients of tea-branch citrus are monoterpenes, preferably α-pinene, α-thujene, β-pinene, and β-myrcene.

[0028] In addition, the present invention also provides a method for promoting the growth of tea-branch oranges or increasing the content of effective ingredients in tea-branch oranges, comprising mixing a bacterial liquid of Streptomyces malachite and a basic fertilizer in a volume ratio of 1:(1-2) and then watering the tea-branch oranges; or applying the above-mentioned biological fertilizer on the tea-branch oranges.

[0029] Preferably, the basic fertilizer adopts a nutrient solution including 1-3 g / L of potassium dihydrogen phosphate, 0.5-1 g / L of ammonium citrate, 0.01-0.05 g / L of calcium chloride, 0.1-0.3 g / L of magnesium sulfate, and 1-3 g / L of sodium chloride.

[0030] Preferably, the bacterial solution of Streptomyces malachite is a bacterial solution of Streptomyces malachite Strep-4 strain, and its concentration is not less than 5×10 11 cfu / L.

[0031] Preferably, the volume ratio of the Streptomyces pomatia bacterial solution to the nutrient solution is 1:1.

[0032] The present invention has the following beneficial effects:

[0033] The present invention provides a bio-fertilizer specifically for tea-branch citrus fruits, comprising Streptomyces malachite-spined and a basic fertilizer containing nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, and sodium. Studies have shown that the combined use of Streptomyces malachite-spined and basic fertilizer exhibits significant synergistic effects, significantly promoting the growth of tea-branch citrus fruits and increasing the content of their active ingredients. Studies have shown that the use of either Streptomyces malachite-spined or basic fertilizer alone is less effective in enhancing the growth and quality of tea-branch citrus fruits than the combined use of the two. Application of either fertilizer or bacterial solution alone increased plant height by 26.66% to 28.23%, chlorophyll by 11.05% to 20.39%, and the content of major monoterpenes by 26.34% to 67.53%. After using the two together, the growth indicators of tea-branch orange were significantly improved, and the content of four major monoterpenes (α-pinene, α-thujacene, β-pinene, and β-myrcene) of tea-branch orange was significantly increased. The plant height of tea-branch orange increased by 57.24%, the chlorophyll increased by 25.74% to 27.53%, and the main monoterpene content increased by 49.79% to 78.35%.

[0034] Therefore, the combination of Streptomyces pomatia and fertilizer promoted the growth and development of the Guangchenpi plants, increased the content of effective ingredients in Guangchenpi, and can further improve the quality of the tea-branch orange. The prepared biological fertilizer was used in the practical operation of artificial cultivation of tea-branch orange, creating conditions for increasing the volatile oil content of tea-branch orange and expanding the planting scale, and can be better applied to tea-branch orange breeding, seedling cultivation, and seed and seedling cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a diagram showing the effects of the experimental groups of Examples 2 to 4 (from left to right in the figure are the control group, the nutrient solution group of Example 2, the bacterial solution group of Example 3, and the combined use group of Example 4). DETAILED DESCRIPTION

[0036] The present invention will be further described below with reference to the accompanying drawings and specific examples, but the examples do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0037] Unless otherwise specified, the reagents and materials used in the following examples were commercially available.

[0038] In particular, in this field, the following embodiments use a combination of nutrient solution + bacterial solution for tea-branch citrus-specific biological fertilizer as one of the preferred embodiments of the present invention. It is also possible to use a bacterial agent containing Streptomyces malachite (such as a freeze-dried powder) in combination with a basic fertilizer (solid fertilizer) containing nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, and sodium nutrients to achieve the effects of the present invention. When used, the OD value of Streptomyces malachite is adjusted to 0.6 to 0.8.

[0039] Example 1 Activation of strains and preparation of bacterial solution

[0040] The Streptomyces sp. Strep-4 strain used in this example was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 30, 2022, with the culture collection number GDMCC NO: 62847, and the storage address is: 5th Floor, Building 59, 100 Xianlie Middle Road, Guangzhou, China.

[0041] Take the malachite spinosa Streptomyces strain stored in glycerol at -80°C, dip a sterile inoculating needle in an appropriate amount of bacterial liquid, and streak it on a solid culture medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, agar 5 g / L). After the culture medium is placed in a 37°C incubator for 24 hours, pick a single colony of malachite spinosa Streptomyces and place it in a liquid culture medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L) and culture until the bacterial liquid has an absorbance of 0.8 at 600 nm on a spectrophotometer. The concentration of the bacterial liquid is 5×10 11 cfu / L.

[0042] Example 2 Effect of nutrient solution on the growth and volatile oil content of tea-branch orange

[0043] Six-month-old tea-branch orange seedlings were selected and planted in nutrient pots of length × width × height = 15cm × 15cm × 10cm. At the same time, the experimental group was set to use liquid fertilizer nutrient solution (2.0g / L potassium dihydrogen phosphate, 0.8g / L ammonium citrate, 0.03g / L calcium chloride, 0.16g / L magnesium sulfate, 2g / L sodium chloride), and the control group was set to use purified water every day. The nutrient solution was watered once every 15 days (purified water was watered at other times). Each group took 30mL and watered it around the roots of the tea-branch orange. The experimental group and the control group were repeated 3 times, with 20 plants in each repeat. After 60 days of watering, the plant height, chlorophyll content, and volatile oil content of the plants in each group were measured.

[0044] The volatile oil extraction process involved thoroughly grinding the leaves with liquid nitrogen, adding 0.8 mL of n-hexane, vortex-mixing, and then subjecting the leaves to ultrasonic extraction. Gas chromatography-mass spectrometry was then used to determine the contents of four volatile components (α-pinene, α-thujene, β-pinene, and β-myrcene) in the volatile oil from each group of tea-branch citrus leaves.

[0045] The results of the effect of nutrient solution on the growth of tea-branch orange are shown in Table 1, which shows that the plant height and leaf chlorophyll content of tea-branch orange seedlings treated with nutrient solution are increased, and are significantly higher than those of the control group, which can promote the growth of tea-branch orange; further determination of the content of active ingredients in the volatile oil of tea-branch orange is shown in Table 2, which shows that after treatment with nutrient solution, the contents of four volatile components in the leaves of tea-branch orange, α-pinene, α-thujene, β-pinene, and β-myrcene, are significantly increased, and are higher than those of the control group.

[0046] Table 1 Growth indicators of tea-branch orange

[0047]

[0048] Table 2 Volatile oil content of tea branch orange

[0049]

[0050] Example 3 Effect of Streptomyces malachite on the growth and volatile oil content of Citrus aurantium

[0051] Select 6-month-old tea branch orange seedlings and plant them in a nutrient basin of length × width × height = 15cm × 15cm × 10cm. Simultaneously, an experimental group is provided with the Streptomyces malachite-spike bacterial solution obtained in Example 1. The control group uses purified water every day, and the bacterial solution group is watered once every 15 days (purified water is poured at other times). Each group takes 30mL and pours it around the roots of the tea branch orange. The experimental group and the control group are 3 replicates, each with 20 plants. After watering the bacteria, co-cultivation is carried out for 60 days, and the tea branch orange plant height, leaf chlorophyll content, and the content of four volatile components (α-pinene, α-thujene, β-pinene, and β-myrcene) are measured. The assay method for the four volatile components is the same as in Example 2.

[0052] The results of growth index measurements, shown in Table 3, demonstrate that the Streptomyces malachite-spike culture solution increased plant height and chlorophyll content in tea-branch citrus trees compared to the control group, demonstrating a growth-promoting effect. The volatile oil content, shown in Table 4, demonstrates that the Streptomyces malachite-spike culture solution increased the contents of α-pinene, α-thujene, β-pinene, and β-myrcene in tea-branch citrus trees.

[0053] Table 3 Growth indicators of tea-branch orange

[0054]

[0055] Table 4 Content of volatile oil in tea branch orange

[0056]

[0057] Example 4 Effect of the combined use of Streptomyces malachite-spined bacterial solution and nutrient solution on the growth of tea-branch orange

[0058] Six-month-old tea-branch orange seedlings were selected and planted in nutrient pots measuring 15 cm long x 15 cm wide x 10 cm high. The experimental group consisted of a mixed bacterial solution of Streptomyces malachite-spined and a nutrient solution mixed in a 1:1 volume ratio. The control group used purified water, with 30 mL of water taken from each group and poured around the roots of the tea-branch orange. Both the experimental and control groups were replicated three times, with 20 plants per replicate. After co-cultivation for 60 days, the tea-branch orange plant height, leaf chlorophyll content, and the contents of four volatile components (α-pinene, α-thujene, β-pinene, and β-myrcene) were measured. The determination method for the four volatile components was the same as in Example 2.

[0059] Table 5 shows the effects of the combined use of Streptomyces malachite-spike culture solution and nutrient solution on the growth indicators of tea-branch orange. This indicates that the mixed culture significantly increased plant height and chlorophyll content in tea-branch orange, demonstrating a significant growth-promoting effect. Table 6 shows the volatile oil content of tea-branch orange, demonstrating that the mixed culture significantly increased the contents of α-pinene, α-thujene, β-pinene, and β-myrcene in tea-branch orange, increasing the active ingredients in tea-branch orange and improving its quality.

[0060] Moreover, combined with the results of Examples 2 to 3, the study showed that the combined use of the Streptomyces malachite-spined bacterial solution and the nutrient solution had a synergistic effect, and its effect was better than using the Streptomyces malachite-spined bacterial solution or the nutrient solution alone. When the nutrient solution was applied alone, the increase rates of plant height, chlorophyll a, chlorophyll b and total chlorophyll were 26.66%, 12.53%, 20.39% and 16.15%, respectively, and the increase rates of α-pinene, α-thujene, β-pinene and β-myrcene were 56.71%, 31.70%, 37.75% and 26.34%, respectively; when the malachite spinulosa Streptomyces liquid was applied alone, the increase rates of plant height, chlorophyll a, chlorophyll b and total chlorophyll were 28.23%, 14.92%, 11.05% and 18.70%, respectively, and the increase rates of α-pinene, α-thujene, β-pinene and β-myrcene were 67.53%, 38.80%, 42.62% and 31.69%, respectively. When the two are used together, they can significantly promote the growth of tea-branch orange and significantly increase the content of effective ingredients in tea-branch orange. The increase rates of plant height, chlorophyll a, chlorophyll b, and total chlorophyll are 57.24%, 25.74%, 27.53%, and 26.57%, respectively. The increase rates of α-pinene, α-thujane, β-pinene, and β-myrcene are 78.35%, 51.22%, 53.92%, and 49.79%, respectively.

[0061] The growth of tea-branch orange plants in different treatment groups Figure 1 As shown, the combined treatment group can significantly promote the growth of tea-branch orange and improve the quality of tea-branch orange. This improvement effect is specifically reflected in the content of monoterpenes. Monoterpenes are the medicinal components of tea-branch orange, and the level of monoterpenes represents the quality of tea-branch orange. After the malachite-spined Streptomyces bacterial solution is used in combination with the nutrient solution, the content of four main monoterpenes (α-pinene, α-thujane, β-pinene, and β-myrcene) is significantly increased, which can significantly improve the quality of tea-branch orange.

[0062] Table 5 Plant height and chlorophyll content of tea-branch orange

[0063]

[0064] Table 6 Tea Branch Citrus Volatile Oil Content

[0065]

[0066] In summary, the present invention combines Streptomyces malachite with a basic fertilizer whose main components are nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium, and finds that the combination of Streptomyces malachite and the basic fertilizer has a significant synergistic effect. When the basic fertilizer or Streptomyces malachite is applied alone, the two have a similar effect on the growth of tea-branch orange, can promote the growth of tea-branch orange and increase the content of the main monoterpenes (α-pinene, α-thujane, β-pinene, β-myrcene) of the effective ingredients of tea-branch orange; when the fertilizer or the bacterial solution is applied alone, the plant height of tea-branch orange is increased by 26.66% to 28.23%, the chlorophyll increase rate is 11.05% to 20.39%, and the main monoterpene content increase rate is 26.34% to 67.53%. The combined use of Streptomyces malachite and fertilizer significantly improved the growth indicators of tea-branch oranges, and the content of the four main monoterpenes (α-pinene, α-thujene, β-pinene, and β-myrcene) of tea-branch oranges was significantly increased. The plant height of tea-branch oranges increased by 57.24%, the chlorophyll increased by 25.74% to 27.53%, and the main monoterpene content increased by 49.79% to 78.35%. Moreover, the combined use also reduced the dosage of both, achieving a significant improvement in the growth indicators of tea-branch oranges and effectively promoting the increase of the effective ingredients in tea-branch oranges. Therefore, the combination of Streptomyces malachite and fertilizer promoted the growth and development of tea-branch orange plants, increased the content of the effective ingredients in tea-branch oranges, and could further improve the quality of tea-branch oranges. The prepared biofertilizer can be used in the practical operation of artificial cultivation of tea-branch oranges, creating conditions for increasing the volatile oil content of tea-branch oranges and expanding the scale of cultivation.

[0067] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be considered as equivalent replacement methods and are included in the scope of protection of the present invention.

Claims

1. A biological fertilizer for tea citrus, characterized in that: The invention comprises Streptomyces malachite and basic fertilizer, wherein the volume ratio of the Streptomyces malachite to the basic fertilizer is 1:(1-2); the mass ratio of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium nutrient elements contained in the basic fertilizer is (135-140):(20-25):(55-60):1:(1-5):(1-5):(155-160); and the OD value of the Streptomyces malachite is 0.6-0.

8.

2. The biological fertilizer according to claim 1, characterized in that The Streptomyces malachite spinulosa is a Streptomyces malachite spinulosa bacterial solution, the concentration of which is not less than 5×10 11 cfu / L.

3. The biological fertilizer according to claim 2, characterized in that The basic fertilizer adopts a nutrient solution, including 1-3 g / L of potassium dihydrogen phosphate, 0.5-1 g / L of ammonium citrate, 0.01-0.05 g / L of calcium chloride, 0.1-0.3 g / L of magnesium sulfate, and 1-3 g / L of sodium chloride.

4. The biological fertilizer according to any one of claims 1 to 3, characterized in that: The Streptomyces sp. is the Streptomyces sp. Strep-4 strain, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 30, 2022, and the culture collection number is GDMCC NO: 62847.

5. Use of the biological fertilizer according to any one of claims 1 to 4 in promoting the growth of tea-branch orange or increasing the content of effective ingredients in tea-branch orange.

6. The application of Streptomyces malachite spinulosa in combination with basic fertilizer in promoting the growth of tea-branch orange or increasing the content of effective ingredients in tea-branch orange, characterized in that: The Streptomyces sp. is the Streptomyces sp. Strep-4 strain, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 30, 2022, with the culture collection number GDMCC NO: 62847; the mass ratio of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium nutrients contained in the basic fertilizer is (135-140): (20-25): (55-60): 1: (1-5): (1-5): (155-160).

7. Use of Streptomyces pomatiae in combination with basic fertilizer in the preparation of a product for promoting the growth of tea-branch orange or increasing the content of effective ingredients in tea-branch orange, characterized in that: The Streptomyces malachite is the Streptomyces malachite (Streptomyces cessp.) Strep-4 strain, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 30, 2022, and the culture collection number is GDMCC NO: 62847; the mass ratio of nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium nutrients contained in the basic fertilizer is (135~140):(20~25):(55~60):1:(1~5):(1~5):(155~160).

8. Use of Streptomyces malachite-spinosa as a fertilizer synergist in promoting the growth of tea-branch orange or increasing the content of effective ingredients in tea-branch orange, characterized in that: The Streptomyces sp. is the Streptomyces sp. Strep-4 strain, which was deposited in the Guangdong Provincial Microbial Culture Collection Center on September 30, 2022, and the culture collection number is GDMCC NO: 62847.

9. A method for promoting the growth of tea-branch citrus or increasing the content of effective ingredients in tea-branch citrus, characterized in that: The bacterial liquid of Streptomyces malachite spinulosa is mixed with basic fertilizer in a volume ratio of 1:(1-2) and then watered on tea-branch oranges; or the biological fertilizer according to any one of claims 1 to 4 is applied to the tea-branch oranges; the mass ratio of the nitrogen, phosphorus, potassium, calcium, magnesium, sulfur and sodium nutrients contained in the basic fertilizer is (135-140):(20-25):(55-60):1:(1-5):(1-5):(155-160).

10. The method according to claim 9, characterized in that: The basic fertilizer adopts a nutrient solution, including 1-3 g / L of potassium dihydrogen phosphate, 0.5-1 g / L of ammonium citrate, 0.01-0.05 g / L of calcium chloride, 0.1-0.3 g / L of magnesium sulfate, and 1-3 g / L of sodium chloride.

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