Flash evaporation drying method of salinomycin fermentation liquor

Abstract

The invention provides a flash evaporation drying method of salinomycin fermentation liquor, and belongs to the technical field of antibiotic production. The preparation method comprises the following steps: preheating salinomycin fermentation liquor, and mixing the preheated salinomycin fermentation liquor with a flocculating agent, diatomite and white carbon black to obtain dispersion liquid; carrying out plate frame filtration on the dispersion liquid to obtain wet mycelia; and carrying out flash evaporation drying on the wet mycelium. After the salinomycin fermentation liquor is preheated, the salinomycin fermentation liquor is mixed with the flocculating agent, the kieselguhr and the white carbon black, and the flocculating agent and the kieselguhr can effectively improve the plate frame filtering speed of the salinomycin fermentation liquor; the salinomycin fermentation liquor contains residual oil (including soybean oil and the content of the residual oil in the salinomycin fermentation liquor is 2-8wt%), and materials are easy to accumulate during flash evaporation, the white carbon black is added, so that the residual oil can be effectively adsorbed, the problems of material accumulation and wall sticking during flash evaporation can be solved, the salinomycin flash evaporation powder with qualified quality can be obtained, and the salinomycin yield can be increased.

Description

Technical Field

[0001] The invention relates to the technical field of antibiotic production, in particular to a flash drying method for salinomycin fermentation liquor. Background Art

[0002] Salinomycin is an antibiotic for animal use, first discovered and isolated by Japanese scientists in 1974 from the fermentation broth of Streptomyces albus. Its structure contains 17 chiral centers. Due to the presence of a carboxylic acid functional group and a polyether chain, it belongs to the monocarboxylic acid polyether antibiotic class. Salinomycin, in its sodium salt form (salinomycin sodium), is commonly added to poultry feed as an anticoccidial drug. Salinomycin sodium also promotes nutrient absorption and improves feeding efficiency in ruminants, and can be used as a fattening agent to promote ruminant growth, leading to its widespread use in many countries.

[0003] The related art discloses that salinomycin fermentation broth is mostly made into granules after pretreatment, but there are few reports on salinomycin fermentation broth being made into flash powder. Spray granulation requires multiple procedures, including spraying, drying, collection and other steps, so the drying efficiency is relatively low; while flash drying can be completed in one operation, with high drying efficiency, and is suitable for situations where mass production is required. In addition, flash drying has lower energy consumption and environmental protection costs than spray granulation. Making flash powder from salinomycin fermentation broth can not only simplify the production process, but also reduce production costs. However, there are two major technical barriers to making flash powder from salinomycin fermentation broth through plate and frame filtration and flash drying. One is that the plate and frame filtration speed of the fermentation broth is not smooth; the other is that it is easy for the material to accumulate and stick to the wall during flash evaporation, making it impossible to produce smoothly.

[0004] In view of the increasing demand for salinomycin, it is necessary to find a method for preparing flash powder from salinomycin fermentation broth. Summary of the Invention

[0005] In view of this, the object of the present invention is to provide a flash drying method for salinomycin fermentation broth. The flash drying method of the present invention has a fast salinomycin plate-frame filtration speed, is not prone to material accumulation and wall adhesion during flash evaporation, and is suitable for industrial production.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a flash drying method for salinomycin fermentation broth, comprising the following steps:

[0008] The salinomycin fermentation liquid is preheated and then mixed with a flocculant, diatomaceous earth and white carbon black to obtain a dispersion liquid;

[0009] The dispersion is subjected to plate-and-frame filtration to obtain wet mycelia;

[0010] The wet mycelia are flash dried.

[0011] Preferably, the temperature of the salinomycin fermentation broth after preheating is 60-80°C.

[0012] Preferably, the dosage of the flocculant is 0.01% to 0.1% of the mass of the salinomycin fermentation broth, and the solid content of the salinomycin fermentation broth is 10% to 15%.

[0013] Preferably, the amount of the flocculant used is 0.05% to 0.08% of the mass of the salinomycin fermentation broth.

[0014] Preferably, the amount of the diatomaceous earth is 0.05% to 5% of the mass of the salinomycin fermentation broth, and the solid content of the salinomycin fermentation broth is 10% to 15%.

[0015] Preferably, the amount of diatomaceous earth used is 0.1% to 4% of the mass of the salinomycin fermentation broth.

[0016] Preferably, the amount of white carbon black is 0.05% to 3% of the mass of the salinomycin fermentation broth, and the solid content of the salinomycin fermentation broth is 10% to 15%.

[0017] Preferably, the amount of white carbon black is 0.15% to 1.5% of the mass of the salinomycin fermentation broth.

[0018] Preferably, the air inlet temperature of the flash drying is 110-130°C, and the air outlet temperature is 55-75°C.

[0019] Preferably, the air inlet temperature of the flash drying is 120-125°C, and the air outlet temperature is 60-70°C.

[0020] The invention provides a flash drying method for salinomycin fermentation broth, comprising the following steps: preheating the salinomycin fermentation broth, mixing it with a flocculant, diatomaceous earth and white carbon black to obtain a dispersion; performing plate-and-frame filtration on the dispersion to obtain wet mycelium; and flash drying the wet mycelium.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention preheats the salinomycin fermentation broth and mixes it with a flocculant, diatomaceous earth, and white carbon black. The flocculant and diatomaceous earth can effectively increase the plate-and-frame filtration speed of the salinomycin fermentation broth. The salinomycin fermentation broth contains residual oil (including soybean oil, with the residual oil content in the salinomycin fermentation broth being 2-8 wt%), which is prone to material accumulation during flash evaporation. The present invention adds white carbon black to effectively absorb the residual oil, solving the problems of material accumulation and wall sticking during flash evaporation, thereby obtaining salinomycin flash powder of qualified quality (low impurity content), and increasing salinomycin production. Furthermore, spray drying consumes relatively high energy consumption, while the present invention uses plate-and-frame filtration combined with flash drying, resulting in lower energy consumption.

[0023] Furthermore, the present invention limits the inlet air temperature of flash drying to 110-130°C and the outlet air temperature to 55-75°C. Salinomycin has a low melting point, and the inlet air temperature is high during flash evaporation, which makes the material easy to soften and stick to the wall. The present invention appropriately lowers the inlet air temperature during flash evaporation, further avoiding the problems of material accumulation and wall sticking during flash evaporation. DETAILED DESCRIPTION

[0024] The present invention provides a flash drying method for salinomycin fermentation broth, comprising the following steps:

[0025] The salinomycin fermentation liquid is preheated and then mixed with a flocculant, diatomaceous earth and white carbon black to obtain a dispersion liquid;

[0026] The dispersion is subjected to plate-and-frame filtration to obtain wet mycelia;

[0027] The wet mycelia are flash dried.

[0028] The invention preheats the salinomycin fermentation liquid and then mixes it with a flocculant, diatomaceous earth and white carbon black to obtain a dispersion.

[0029] The present invention has no particular limitation on the source of the salinomycin fermentation broth, and any source well known to those skilled in the art may be used.

[0030] In the present invention, the solid content of the salinomycin fermentation broth is preferably 10% to 15%, that is, the solid content of the mycelium in the salinomycin fermentation broth is preferably 10 to 15 wt%, and the residual oil content is preferably 2 to 8 wt%, specifically 2, 3, 4, 5, 6, 7 or 8 wt%.

[0031] In the present invention, the temperature of the salinomycin fermentation broth after preheating is preferably 60-80°C, specifically 60, 65, 70, 75 or 80°C; the preheating can be.

[0032] In the present invention, the preheating is preferably steam heating.

[0033] In the present invention, the dosage of the flocculant is preferably 0.01% to 0.1% of the mass of the salinomycin fermentation broth, more preferably 0.05% to 0.08%, specifically 0.01%, 0.05%, 0.07%, 0.08% or 0.1%.

[0034] In the present invention, the flocculant preferably includes aluminum sulfate, which is cheap and low in cost.

[0035] In the present invention, the amount of diatomaceous earth is preferably 0.05% to 5% of the mass of the salinomycin fermentation broth, more preferably 0.1% to 4%, specifically 0.05%, 0.08%, 1%, 2%, 2.9%, 3%, 4% or 5%.

[0036] In the present invention, the particle size of the diatomaceous earth is preferably 5 to 60 μm, more preferably 10 to 30 μm, specifically 5, 10, 30, 40, 50 or 60 μm.

[0037] In the present invention, the flocculant and diatomaceous earth can effectively improve the plate-and-frame filtration speed of salinomycin fermentation liquid; if the amount of flocculant and diatomaceous earth added is too little, the flocculation effect cannot be achieved, resulting in a too slow plate-and-frame filtration speed; if too much flocculant is added, the pH value of the fermentation liquid is low, affecting product quality.

[0038] In the present invention, the amount of the white carbon black is preferably 0.05% to 3% of the mass of the salinomycin fermentation broth, more preferably 0.15% to 1.5%, specifically 0.05%, 0.15%, 0.5%, 1.5% or 3%; the white carbon black can effectively adsorb residual oil and solve the problems of material accumulation and wall sticking during flash evaporation. If too little white carbon black is added, it is easy to stick to the wall during flash evaporation. If too much white carbon black is added, the specific gravity of the product is affected, thereby reducing the product quality.

[0039] In the present invention, the particle size of the white carbon black is preferably 10 to 100 nm, more preferably 20 to 50 nm, and specifically may be 10, 20, 40, 50, 60, 80 or 100 nm.

[0040] In the present invention, the salinomycin fermentation liquid is preferably heated first, a flocculant is added, the diatomaceous earth and white carbon black are added, and then mechanical stirring is performed to obtain the dispersion.

[0041] In the present invention, the rotation speed of the mechanical stirring is preferably 100 to 800 rpm, preferably 200 to 600 rpm, and the time is preferably 0.5 to 1 h.

[0042] After obtaining the dispersion, the present invention performs plate and frame filtration on the dispersion to obtain wet mycelium.

[0043] In the present invention, the plate and frame filtration rate is preferably 1 to 10 m 3 / h, specifically 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10m 3 / h.

[0044] After obtaining the wet mycelia, the present invention performs flash drying on the wet mycelia.

[0045] In the present invention, the inlet air temperature of the flash drying is preferably 110-130°C, more preferably 120-125°C, specifically 110, 115, 120, 125 or 130°C, and the outlet air temperature is preferably 55-75°C, more preferably 60-70°C, specifically 55, 60, 65, 70 or 75°C; salinomycin has a low melting point, and the inlet air temperature is high during flash evaporation, so the material tends to soften and stick to the wall. The present invention appropriately reduces the inlet air temperature during flash evaporation, further avoiding the problems of material accumulation and wall sticking during flash evaporation.

[0046] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions of the present invention. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0047] The solid content of the mycelium of the salinomycin fermentation broth used in the embodiment of the present invention is 12 wt %, and the content of residual oil is 8 wt %; the particle size of the diatomaceous earth is 20 μm, and the particle size of the white carbon black is 40 nm.

[0048] Yield = mass of salinomycin flash powder / (mass of mycelium in salinomycin fermentation broth + mass of flocculant + mass of diatomaceous earth + mass of white carbon black) × 100%

[0049] Example 1

[0050] (1) Plate and frame filtration: 96,000 kg of salinomycin fermentation broth was steam heated to 80 °C, 48 kg of aluminum sulfate was added, followed by 960 kg of diatomaceous earth and 48 kg of white carbon black, and mechanical stirring was performed for 0.5 h. The plate and frame filtration rate was 6 m 3 / h, to obtain wet mycelium;

[0051] (2) Flash drying: The air inlet temperature was controlled at 110°C and the air outlet temperature was controlled at 55°C to obtain 11318 kg of salinomycin flash powder with a specific gravity of 0.45 g / mL and a yield of 90%. The content of salinomycin in the salinomycin flash powder was 37.04 wt%.

[0052] Example 2

[0053] (1) Plate and frame filtration: 94000 kg of salinomycin fermentation liquid was steam heated to 70 °C, 94 kg of aluminum sulfate was added, followed by 1880 kg of diatomaceous earth and 470 kg of white carbon black, mechanically stirred for 0.5 h, and plate and frame filtered (7 m 3 / h), to obtain wet mycelium;

[0054] (2) Flash drying: The air inlet temperature was controlled at 115°C and the air outlet temperature was controlled at 60°C to obtain 12557 kg of salinomycin flash powder with a specific gravity of 0.48 g / mL and a yield of 91.5%. The content of salinomycin in the salinomycin flash powder was 34.48 wt%.

[0055] Example 3

[0056] (1) Plate and frame filtration: 95000 kg of salinomycin fermentation liquid was steam heated to 75 °C, 9.5 kg of aluminum sulfate was added, followed by 2820 kg of diatomaceous earth and 142.5 kg of white carbon black, mechanically stirred for 0.5 h, and plate and frame filtration (7.5 m 3 / h), to obtain wet mycelium;

[0057] (2) Flash drying: The air inlet temperature was controlled at 120°C and the air outlet temperature was controlled at 65°C to obtain 13078 kg of salinomycin flash powder with a specific gravity of 0.53 g / mL and a yield of 91%. The content of salinomycin in the salinomycin flash powder was 32.26 wt%.

[0058] Example 4

[0059] (1) Plate and frame filtration: 98,000 kg of salinomycin fermentation liquid was steam heated to 80 ° C, 78.4 kg of aluminum sulfate was added, followed by 3920 kg of diatomaceous earth and 2940 kg of white carbon black, mechanically stirred for 0.5 h, and plate and frame filtered (8.5 m 3 / h), to obtain wet mycelium;

[0060] (2) Flash drying: The air inlet temperature was controlled at 125°C and the air outlet temperature was controlled at 70°C to obtain 17202 kg of salinomycin flash powder with a specific gravity of 0.5 g / mL and a yield of 92%. The content of salinomycin in the salinomycin flash powder was 26.32 wt%.

[0061] Example 5

[0062] (1) Plate and frame filtration: 96,000 kg of salinomycin fermentation liquid was steam heated to 60 ° C, 67.2 kg of aluminum sulfate was added, and then 3840 kg of diatomaceous earth and 1440 kg of white carbon black were added. The mixture was mechanically stirred for 0.5 h and then filtered through a plate and frame (8.5 m 3 / h), to obtain wet mycelium;

[0063] (2) Flash drying: The air inlet temperature was controlled at 115°C and the air outlet temperature was controlled at 60°C to obtain 15687 kg of salinomycin flash powder with a specific gravity of 0.49 g / mL and a yield of 93%. The content of salinomycin in the salinomycin flash powder was 28.57 wt%.

[0064] Comparative Example 1

[0065] Same as Example 1, except that no aluminum sulfate was added and the plate and frame filtration rate was 5.5 m 3 / h.

[0066] 10648 kg of salinomycin flash powder was obtained, with a specific gravity of 0.45 g / mL and a yield of 85%. The content of salinomycin in the salinomycin flash powder was 38.46 wt%.

[0067] Comparative Example 2

[0068] Same as Example 1, except that diatomaceous earth was not added and the plate and frame filtration rate was 4.5 m 3 / h.

[0069] 10185 kg of salinomycin flash powder was obtained, with a specific gravity of 0.44 g / mL and a yield of 88%. The content of salinomycin in the salinomycin flash powder was 39.68 wt%.

[0070] Comparative Example 3

[0071] Same as Example 1, except that no white carbon black is added and the plate and frame filtration rate is 3.5m 3 / h.

[0072] 11137 kg of salinomycin flash powder was obtained, with a specific gravity of 0.55 g / mL and a yield of 89%. The content of salinomycin in the salinomycin flash powder was 33.3 wt%.

[0073] Comparative Example 4

[0074] (1) Plate and frame filtration: 96,000 kg of salinomycin fermentation liquid was steam heated to 80 ° C, 480 kg of aluminum sulfate was added, and then 6720 kg of diatomaceous earth and 3840 kg of white carbon black were added. The mixture was mechanically stirred for 0.5 h and then plate and frame filtered (4.5 m 3 / h), to obtain wet mycelium;

[0075] (2) Flash drying: The air inlet temperature was controlled at 110°C and the air outlet temperature was controlled at 55°C to obtain 19176 kg of salinomycin flash powder with a specific gravity of 0.51 g / mL and a yield of 85%. The content of salinomycin in the salinomycin flash powder was 21.28 wt%.

[0076] Comparative Example 5

[0077] (1) Plate and frame filtration: 96000 kg of salinomycin fermentation liquid was steam heated to 80 °C, 4.8 kg of aluminum sulfate was added, followed by 9.6 kg of diatomaceous earth and 9.6 kg of white carbon black, and mechanical stirring was performed for 0.5 h. Plate and frame filtration (3 m 3 / h), to obtain wet mycelium;

[0078] (2) Flash drying: The air inlet temperature was controlled at 110°C and the air outlet temperature was controlled at 55°C to obtain 9232 kg of salinomycin flash powder with a specific gravity of 0.53 g / mL and a yield of 80%. The content of salinomycin in the salinomycin flash powder was 41.32 wt%.

[0079] Comparative Example 6

[0080] The same as Example 1, the only difference is that the inlet air temperature is controlled at 135°C and the outlet air temperature is controlled at 50°C.

[0081] 10689 kg of salinomycin flash powder was obtained, with a specific gravity of 0.45 g / mL and a yield of 85%. The content of salinomycin in the salinomycin flash powder was 37 wt%.

[0082] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation thereto. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications shall also be considered within the scope of protection of the present invention.

Claims

1. A flash drying method for salinomycin fermentation broth, characterized in that: The following steps are involved: The salinomycin fermentation liquid is preheated and then mixed with a flocculant, diatomaceous earth and white carbon black to obtain a dispersion liquid; The dispersion is subjected to plate-and-frame filtration to obtain wet mycelia; The wet mycelia are flash dried.

2. The flash drying method according to claim 1, wherein The temperature of the salinomycin fermentation liquid after preheating is 60-80°C.

3. The flash drying method according to claim 1, wherein The dosage of the flocculant is 0.01% to 0.1% of the mass of the salinomycin fermentation liquid, and the solid content of the salinomycin fermentation liquid is 10% to 15%.

4. The flash drying method according to claim 3, wherein The dosage of the flocculant is 0.05% to 0.08% of the mass of the salinomycin fermentation liquid.

5. The flash drying method according to claim 1, wherein The dosage of the diatomaceous earth is 0.05% to 5% of the mass of the salinomycin fermentation liquid, and the solid content of the salinomycin fermentation liquid is 10% to 15%.

6. The flash drying method according to claim 5, characterized in that The dosage of the diatomaceous earth is 0.1% to 4% of the mass of the salinomycin fermentation liquid.

7. The flash drying method according to claim 1, wherein The amount of white carbon black used is 0.05% to 3% of the mass of the salinomycin fermentation liquid, and the solid content of the salinomycin fermentation liquid is 10% to 15%.

8. The flash drying method according to claim 7, characterized in that The amount of white carbon black used is 0.15% to 1.5% of the mass of the salinomycin fermentation liquid.

9. The flash drying method according to claim 1, wherein: The air inlet temperature of the flash drying is 110-130°C, and the air outlet temperature is 55-75°C.

10. The flash drying method according to claim 9, characterized in that: The air inlet temperature of the flash drying is 120-125°C, and the air outlet temperature is 60-70°C.