Human mesenchymal stem cell separation device

Abstract

The invention discloses a human mesenchymal stem cell separation device which comprises a machine body, a taking and placing cavity and a working cavity are formed in the machine body, a through hole is formed between the taking and placing cavity and the working cavity, a feeding centrifugal mechanism is arranged between the taking and placing cavity and the working cavity, and a transparent window is arranged on the front side of the working cavity. According to the human mesenchymal stem cell separation device, a test tube is put into a test tube rack, then a winding motor pulls a counterweight base to move through a connecting rope, the test tube is fed into a working cavity to be separated, after separation, the winding motor releases the connecting rope, and the counterweight base descends through the gravity of the counterweight base; the test tube rack is sent into the taking and placing cavity from the working cavity, the through hole is covered with the top plate, test tubes can be taken out conveniently, cold air emission is reduced in the taking-out process, fluctuation of the temperature in the working cavity is small, the cooling frequency of continuous centrifugation is reduced, and therefore use convenience can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell separation, and in particular to a human mesenchymal stem cell separation device. Background Art

[0002] Human mesenchymal stem cells (hMSCs) are a type of pluripotent stem cell that possess all the common properties of stem cells, namely, self-renewal and multidirectional differentiation capabilities. They are also the most widely used in clinical applications. Combined use with hematopoietic stem cells can improve the success rate of transplantation and accelerate hematopoietic reconstruction. The isolation of hMSCs is a fundamental step in stem cell research. A centrifuge is used to centrifuge test tubes containing hMSCs. However, in existing human mesenchymal stem cell separation devices, it is not convenient to remove the test tubes from the body after centrifugation. In addition, when removing the test tubes, the cold air inside will be emitted outward, causing the internal temperature to rise. Continuous centrifugation requires further cooling, making the tubes less convenient to use.

[0003] For example, patent publication number CN217838921U specifically discloses a mesenchymal stem cell separation and extraction device, which solves the problem in the prior art that when a centrifuge drives cells to rotate rapidly, the temperature of the cells will rise. When the temperature rises, the cell activity will decrease. In particular, mesenchymal stem cells are extremely sensitive to temperature. The temperature rise will cause deviations in the final research results. At the same time, the centrifugal device will generate certain noise during operation. A mesenchymal stem cell separation and extraction device includes a box body, an inner frame is fixedly connected to the inner side of the box body, and a certain gap is formed between the inner frame and the box body. The method proposed in this utility model uses a liquid nitrogen tank to transport liquid nitrogen gas into the inner frame, thereby cooling the mesenchymal stem cells during centrifugation to ensure the activity of the mesenchymal stem cells. At the same time, the noise generated during centrifugation is isolated by using sound insulation cotton between the box body and the inner frame. However, there is a problem that after centrifuging the cells, it is inconvenient to remove the test tubes in the body, and when removing the test tubes, the cold air inside will be emitted outward, causing the internal temperature to rise. Continuous centrifugation requires cooling again, making the convenience of use less than ideal. Summary of the Invention

[0004] (1) Technical problems solved

[0005] In response to the shortcomings of the existing technology, the present invention provides a human mesenchymal stem cell separation device, which solves the problem that after the human mesenchymal stem cell separation device centrifuges the cells, it is inconvenient to remove the test tube inside the body. When removing the test tube, the cold air inside will be emitted outward, causing the internal temperature to rise. During continuous centrifugation, the temperature needs to be lowered again, making it less convenient to use.

[0006] (2) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: A human mesenchymal stem cell separation device includes a body, a loading and unloading chamber and a working chamber are provided within the body, a through hole is formed between the loading and unloading chamber and the working chamber, a feeding centrifuge mechanism is provided between the loading and unloading chamber and the working chamber, a transparent window is provided on the front side of the working chamber, a disinfection shielding mechanism is provided on one side of the transparent window, a cooling mechanism and a central control box are provided on both sides of the body, and a touch screen is installed on the central control box;

[0008] The top end face of said sliding arm is fixedly provided with a toothed connecting strip which is cooperatively connected with said toothed connecting gear.

[0009] Preferably, the cross-sectional areas of the counterweight base and the top plate are both larger than the cross-sectional area of the through hole, and sealing rings are provided on the surfaces on opposite sides of the counterweight base and the top plate to increase air tightness, thereby reducing cold air loss.

[0010] Preferably, the driven gear matches the driving gear, and the fixing seat is fixedly installed on the top of the inner wall of the pick-and-place cavity, so that the driving gear can mesh with the driven gear.

[0011] Preferably, the driven gear is arranged at the bottom of the counterweight base, and the opening matches the driving gear, so that the driving gear can enter the bottom of the counterweight base through the opening.

[0012] Preferably, the two telescopic guide rods are symmetrically arranged on both sides of the electric push rod, and the two ends of the telescopic guide rod and the electric push rod are fixedly connected to the fixed seat and the movable seat respectively, so that the telescopic guide rod can be used to play a guiding and supporting role, and the electric push rod can be used to drive the movable seat to move.

[0013] Preferably, the support seat is installed inside the pick-and-place cavity, and the output shaft end of the winding motor is connected to the winding shaft through a worm and a worm gear, so that the winding motor can drive the winding shaft to rotate.

[0014] Preferably, the disinfection and shielding mechanism includes an ultraviolet disinfection lamp and a storage box, the storage box is rotatably connected to a rotating shaft inside, a spring is installed at one end of the rotating shaft, a blackout cloth is fixedly connected to the rotating shaft, the bottom end of the blackout cloth is fixedly connected to an extension rod, the end of the extension rod is fixedly connected to a pull rope, the end of the pull rope is fixedly connected to a pull ring, the pull ring is fixedly installed on the top plate, the cross-sectional shape of the extension rod is set to T-shaped, and the storage box is fixedly installed on the top of the machine body, so that the inside of the working chamber can be disinfected and the transparent window can be shielded to prevent ultraviolet rays from causing harm to people around.

[0015] Preferably, the cooling mechanism includes a refrigerator, a circulation pipe is connected to the end of the refrigerator, a heat exchange coil is connected to the end of the circulation pipe, a mounting bracket is provided on the outside of the heat exchange coil, and the cooling mechanism also includes a temperature sensor, and the mounting bracket and the temperature sensor are both fixedly installed inside the working chamber, so that the temperature inside the working chamber can be maintained within a certain temperature range, thereby enabling the cells to be at a suitable temperature and ensuring cell activity.

[0016] The present invention provides a human mesenchymal stem cell separation device. Compared with the prior art, it has the following advantages:

[0017] Beneficial effects:

[0018] (1) The human mesenchymal stem cell separation device places a test tube into a test tube rack, and then uses a winding motor to pull the counterweight base to move by using a connecting rope to send the test tube into the working chamber for separation. After separation, the winding motor releases the connecting rope, and the counterweight base descends by its own gravity, and sends the test tube rack from the working chamber to the inside of the placement chamber, and uses a top plate to cover the through hole, so that the test tube is easy to take out and the emission of cold air is reduced during the removal process. The temperature fluctuation inside the working chamber is small, and the number of cooling times of continuous centrifugation is reduced, thereby effectively improving the convenience of use.

[0019] (2) The human mesenchymal stem cell separation device drives the pull ring to move through the top plate, the pull ring moves to move the pull rope, the pull rope moves to pull the T-shaped extension rod, the extension rod moves to drive the blackout cloth to move, the blackout cloth is pulled out of the storage box to block the transparent window, and then the ultraviolet disinfection lamp is turned on to perform ultraviolet disinfection on the inside of the working chamber, so that the inside of the working chamber can be disinfected and the transparent window can be blocked to prevent ultraviolet rays from causing harm to people around.

[0020] (3) The human mesenchymal stem cell separation device monitors the temperature inside the working chamber through a temperature sensor and uses a programmable PLC to control the refrigerator to operate. The refrigerator circulates the cooling medium so that it flows into the heat exchange coil and cools the temperature inside the working chamber, so that the temperature inside the working chamber can be maintained within a certain temperature range, thereby keeping the cells at a suitable temperature and ensuring cell activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a structural schematic diagram of the feeding centrifugal mechanism of the present invention;

[0023] Figure 3 This is a schematic structural diagram of the fixing seat and driven gear of the present invention;

[0024] Figure 4 It is a schematic diagram of the support base structure of the present invention;

[0025] Figure 5 Schematic diagram of the structure of the disinfection shielding mechanism of the present invention;

[0026] Figure 6 It is a schematic structural diagram of the cooling mechanism of the present invention.

[0027] In the figure: 1. body; 2. centrifugal feeding mechanism; 201. counterweight base; 202. connecting rod; 203. top plate; 204. sealing ring; 205. opening; 206. test tube rack; 207. driven gear; 208. movable seat; 209. driving gear; 210. driving motor; 211. fixed seat; 212. telescopic guide rod; 213. electric push rod; 214. connecting rope; 215. pulley; 216. reel; 217. worm gear; 218. worm ; 219. Winding motor; 220. Support seat; 3. Pick-up and placement chamber; 4. Working chamber; 5. Through hole; 6. Disinfection shielding mechanism; 601. Storage box; 602. Ultraviolet disinfection lamp; 603. Rotating shaft; 604. Blackout cloth; 605. Pull rope; 606. Spring; 607. Pull ring; 7. Cooling mechanism; 701. Refrigeration machine; 702. Circulation pipe; 703. Mounting bracket; 704. Heat exchange coil; 705. Temperature sensor; 8. Central control box; 9. Touch screen. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0029] See also Figure 1-4 , the embodiment of the present invention provides a technical solution: a human mesenchymal stem cell separation device, comprising a body 1, a take-and-place chamber 3 and a working chamber 4 are provided inside the body 1, a through hole 5 is opened between the take-and-place chamber 3 and the working chamber 4, a feeding centrifugal mechanism 2 is provided between the take-and-place chamber 3 and the working chamber 4, a transparent window is provided on the front side of the working chamber 4, a disinfection shielding mechanism 6 is provided on one side of the transparent window, a cooling mechanism 7 and a central control box 8 are provided on both sides of the body 1, a programmable PLC is installed inside the central control box 8, which can control the whole, a touch screen 9 is installed on the central control box 8, the touch screen 9 can facilitate the operation of the staff, and can display the corresponding working parameters, such as temperature, the test tube can be centrifuged by the feeding centrifugal mechanism 2 to separate the human mesenchymal stem cells, and the test tube can be conveniently taken out, and the cold air emission is reduced during the removal process, the temperature fluctuation inside the working chamber 4 is small, and the number of cooling times of continuous centrifugation is reduced, thereby effectively improving the convenience of use.

[0030] The feeding centrifugal mechanism 2 includes a counterweight base 201 and a fixed seat 211. The top of the counterweight base 201 is fixedly connected to a connecting rod 202. The connecting rod 202 is arranged to penetrate the inner wall of the body 1, so that it can guide the movement of the counterweight base 201 and the top plate 203. The top of the connecting rod 202 is fixedly connected to the top plate 203. The cross-sectional area of the counterweight base 201 and the top plate 203 is larger than the cross-sectional area of the through hole 5. A sealing ring 204 is provided on the surface of the opposite side of the counterweight base 201 and the top plate 203 to increase the air tightness, thereby reducing the loss of cold air. An opening 205 is provided on the counterweight base 201, and a test tube rack is rotatably connected between the counterweight base 201 and the top plate 203. 206, the bottom end of the test tube rack 206 is fixedly connected to a driven gear 207, a movable seat 208 is provided on one side of the fixed seat 211, and a telescopic guide rod 212 and an electric push rod 213 are provided between the fixed seat 211 and the movable seat 208. The two telescopic guide rods 212 are symmetrically arranged on both sides of the electric push rod 213, and the ends of the telescopic guide rod 212 and the electric push rod 213 are respectively fixedly connected to the fixed seat 211 and the movable seat 208, so that the telescopic guide rod 212 can be used to play a guiding and supporting role, and the electric push rod 213 can be used to drive the movable seat 208 to move. The movable seat 208 is rotatably connected to a driving gear 209, and the driven gear 207 is set at the bottom of the counterweight base 201. The opening 205 Matching with the driving gear 209, the driving gear 209 can enter the bottom of the counterweight base 201 through the opening 205, the driven gear 207 matches the driving gear 209, and the fixing seat 211 is fixedly installed on the top of the inner wall of the pick-and-place chamber 3, so that the driving gear 209 can mesh with the driven gear 207. The bottom end of the driving gear 209 is fixedly connected to the driving motor 210, and the top of the top plate 203 is fixedly connected to the connecting rope 214. The end of the connecting rope 214 passes through the working chamber 4 and extends to the inside of the pick-and-place chamber 3, and an elastic material is installed at the penetration position for sealing. The elastic material can be made of rubber to fill the gap. A pulley 215 is provided on one side of the connecting rope 214, and the pulley 215 is fixedly installed in the working chamber 4. Internally, the pulley 215 can be used to guide the connecting rope 214 very well. The bottom end of the connecting rope 214 is fixedly connected to the winding shaft 216, and the outer side of the winding shaft 216 is rotatably connected to the support seat 220. The end of the winding shaft 216 is fixedly connected to a worm gear 217, and a worm 218 is provided on one side of the worm gear 217. The self-locking function of the worm gear 217 and the worm gear 218 can lock the winding shaft 216. The end of the worm gear 218 is fixedly connected to a winding motor 219, and the support seat 220 is installed inside the pick-and-place chamber 3, and the output shaft end of the winding motor 219 is connected to the winding shaft 216 through the worm gear 218 and the worm gear 217, so that the winding motor 219 can drive the winding shaft 216 to rotate.

[0031] See also Figure 1 and Figure 5The disinfection shielding mechanism 6 includes an ultraviolet disinfection lamp 602 and a storage box 601. The storage box 601 is internally connected to a rotating shaft 603. A spring 606 is installed at one end of the rotating shaft 603. The spring 606 can reset the rotating shaft 603 so that the blackout cloth 604 can be automatically reeled up. The rotating shaft 603 is fixedly connected to a blackout cloth 604. The blackout cloth 604 can block ultraviolet rays and prevent ultraviolet rays from passing through the transparent window to the outside. The bottom end of the blackout cloth 604 is fixedly connected to an extension rod, and the end of the extension rod is fixedly connected to a pull rope 605. The end of the pull rope 605 is fixedly connected to a pull ring 607. The pull ring 6 07 is fixedly mounted on the top plate 203, the cross-sectional shape of the extension rod is set to T-shaped, and the storage box 601 is fixedly mounted on the top of the body 1. The pull ring 607 can be driven to move by the top plate 203, and the movement of the pull ring 607 drives the pull rope 605 to move. The movement of the pull rope 605 pulls the T-shaped extension rod, and the movement of the extension rod drives the blackout cloth 604 to move, and the blackout cloth 604 is pulled out of the storage box 601 to block the transparent window, and then the ultraviolet disinfection lamp 602 is turned on to perform ultraviolet disinfection on the inside of the working chamber 4, so that the inside of the working chamber 4 can be disinfected and the transparent window can be blocked to prevent ultraviolet rays from causing harm to people around.

[0032] See also Figure 1 and Figure 6 The cooling mechanism 7 includes a refrigerator 701, which can drive the cooling medium to circulate and use the cooling medium to perform heat exchange and cooling. The end of the refrigerator 701 is connected to a circulation pipe 702, and the end of the circulation pipe 702 is connected to a heat exchange coil 704. A mounting bracket 703 is provided on the outside of the heat exchange coil 704. The cooling mechanism 7 also includes a temperature sensor 705. The temperature sensor 705 is a DS18B20 model and can monitor the temperature inside the working chamber 4 in real time. The mounting bracket 703 and the temperature sensor 705 are both fixedly installed inside the working chamber 4. The temperature inside the working chamber 4 can be monitored by the temperature sensor 705, and the programmable PLC is used to control the refrigerator 701 to work. The refrigerator 701 circulates the cooling medium so that it flows into the heat exchange coil 704, and cools down the temperature inside the working chamber 4, so that the temperature inside the working chamber 4 can be maintained within a certain temperature range, thereby enabling the cells to be at a suitable temperature and ensuring cell activity.

[0033] When in use, the test tube is placed inside the test tube rack 206, and then the winding motor 219 drives the worm 218 to rotate, the rotation of the worm 218 drives the worm gear 217 to rotate, the rotation of the worm gear 217 drives the winding shaft 216 to rotate, and the winding shaft 216 rotates to reel the connecting rope 214, and the connecting rope 214 is used to pull the counterweight base 201 to move, and the test tube is sent into the working chamber 4, and then the electric push rod 213 sends the driving gear 209 to the bottom of the counterweight base 201, so that the driving gear 209 is engaged with the driven gear 207, and then the driving motor 210 drives the driving gear 209 to rotate. The driving gear 209 rotates to drive the driven gear 207 to rotate, and the driven gear 207 rotates to drive the test tube rack 206 to rotate for separation. After separation, the electric push rod 213 retracts, and the winding motor 219 rotates in the opposite direction to release the connecting rope 214. The counterweight base 201 uses its own gravity to descend, and the test tube rack 206 is sent from the working chamber 4 to the inside of the loading and unloading chamber 3, and the top plate 203 is used to cover the through hole 5, so that the test tube is convenient to take out and the emission of cold air is reduced during the removal process. The temperature fluctuation inside the working chamber 4 is small, and the number of cooling times of continuous centrifugation is reduced, thereby effectively improving the convenience of use.

[0034] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0035] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0036] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A human mesenchymal stem cell separation device, comprising a body (1), characterized in that: The body (1) is provided with a take-and-place chamber (3) and a working chamber (4), a through hole (5) is provided between the take-and-place chamber (3) and the working chamber (4), a feeding centrifugal mechanism (2) is provided between the take-and-place chamber (3) and the working chamber (4), a transparent window is provided on the front side of the working chamber (4), a disinfection shielding mechanism (6) is provided on one side of the transparent window, a cooling mechanism (7) and a central control box (8) are provided on both sides of the body (1), and a touch screen (9) is installed on the central control box (8); The feeding centrifugal mechanism (2) comprises a counterweight base (201) and a fixed base (211), the top of the counterweight base (201) is fixedly connected to a connecting rod (202), the top of the connecting rod (202) is fixedly connected to a top plate (203), an opening (205) is provided on the counterweight base (201), a test tube rack (206) is rotatably connected between the counterweight base (201) and the top plate (203), the bottom end of the test tube rack (206) is fixedly connected to a driven gear (207), a movable base (208) is provided on one side of the fixed base (211), a telescopic guide rod (212) and an electric push rod (213) are provided between the fixed base (211) and the movable base (208), and a driving gear (207) is rotatably connected to the movable base (208). 09), the bottom end of the driving gear (209) is fixedly connected to a driving motor (210), the top of the top plate (203) is fixedly connected to a connecting rope (214), the end of the connecting rope (214) passes through the working chamber (4) and extends to the inside of the pick-up and release chamber (3), a pulley (215) is provided on one side of the connecting rope (214), and the pulley (215) is fixedly installed inside the working chamber (4), the bottom end of the connecting rope (214) is fixedly connected to a winding shaft (216), the outer side of the winding shaft (216) is rotatably connected to a support seat (220), the end of the winding shaft (216) is fixedly connected to a worm wheel (217), one side of the worm wheel (217) is provided with a worm (218), and the end of the worm (218) is fixedly connected to a winding motor (219).

2. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The cross-sectional areas of the counterweight base (201) and the top plate (203) are both larger than the cross-sectional area of the through hole (5), and sealing rings (204) are provided on the surfaces of opposite sides of the counterweight base (201) and the top plate (203).

3. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The driven gear (207) matches the driving gear (209), and the fixing seat (211) is fixedly mounted on the top of the inner wall of the taking-and-putting cavity (3).

4. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The driven gear (207) is arranged at the bottom of the counterweight base (201), and the opening (205) matches the driving gear (209).

5. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The two telescopic guide rods (212) are symmetrically arranged on both sides of the electric push rod (213), and the two ends of the telescopic guide rod (212) and the electric push rod (213) are fixedly connected to the fixed seat (211) and the movable seat (208) respectively.

6. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The support seat (220) is installed inside the take-and-place chamber (3), and the output shaft end of the winding motor (219) is transmission-connected to the winding shaft (216) through a worm (218) and a worm wheel (217).

7. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The disinfection shielding mechanism (6) comprises an ultraviolet disinfection lamp (602) and a storage box (601); a rotating shaft (603) is rotatably connected inside the storage box (601); a spring (606) is installed at one end of the rotating shaft (603); a blackout cloth (604) is fixedly connected to the rotating shaft (603); an extension rod is fixedly connected to the bottom end of the blackout cloth (604); a pull rope (605) is fixedly connected to the end of the extension rod; and a pull ring (607) is fixedly connected to the end of the pull rope (605).

8. The human mesenchymal stem cell separation device according to claim 7, characterized in that: The pull ring (607) is fixedly mounted on the top plate (203), the cross-sectional shape of the extension rod is set to be T-shaped, and the storage box (601) is fixedly mounted on the top of the body (1).

9. The human mesenchymal stem cell separation device according to claim 1, characterized in that: The cooling mechanism (7) comprises a refrigerator (701), the end of the refrigerator (701) is connected to a circulation pipe (702), the end of the circulation pipe (702) is connected to a heat exchange coil (704), and a mounting frame (703) is provided on the outside of the heat exchange coil (704).

10. The human mesenchymal stem cell separation device according to claim 9, characterized in that: The cooling mechanism (7) further comprises a temperature sensor (705), and the mounting frame (703) and the temperature sensor (705) are both fixedly mounted inside the working chamber (4).

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