Burkholderia sp. SJ08 strain, microbial inoculum as well as preparation method and application of Burkholderia sp. SJ08 strain

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a Burkholderia sp. SJ08 strain, a Burkholderia sp. SJ08 fungicide, and a preparation method and application thereof. The invention discloses a strain of Burkholderia cepacia SJ08, the Burkholderia cepacia SJ08 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC (China General Microbiological Culture Collection Center): 65908, and the preservation date is February 18, 2025. The Burkholderia sp. SJ08 strain is screened from seed radix pseudostellariae, crop diseases caused by fusarium oxysporum can be effectively inhibited, continuous cropping obstacles of crops can be inhibited, crop growth is promoted, and the Burkholderia sp. SJ08 strain is safe and free of pollution. The Burkholderia sp. SJ08 strain provided by the invention has a good application prospect in crop production.

Description

Technical Field

[0001] The present invention belongs to the field of microbial technology, and specifically relates to a Burkholderia SJ08 strain, a bacterial agent, and a preparation method and application thereof. Background Art

[0002] Pseudostellariae Radix is also known as baby ginseng and child ginseng. It is the tuberous root of the perennial herbaceous plant Stellaria heterophylla of the Caryophyllaceae family. Pseudostellariae Radix was first recorded in the "Compendium of Materia Medica" and is currently included in the list of Chinese medicinal materials that can be used in health foods. Pseudostellariae Radix is a tonic herb suitable for treating spleen and lung deficiency, qi and yin deficiency, and qi and fluid deficiency. Pseudostellariae Radix contains a variety of trace elements, cyclic peptides, amino acids, sugars, glycosides, phospholipids, fatty acids, sterols and other chemical components. It has a good effect on improving myocardial ischemia, diabetes mellitus, and memory. Therefore, artificial cultivation of Pseudostellariae Radix has good application prospects.

[0003] However, during the cultivation of Pseudostellaria chinensis, serious soil-borne diseases often occur, which in turn leads to reduced production and quality of Pseudostellaria chinensis. Soil-borne diseases seriously restrict the development of the Pseudostellaria chinensis industry. Currently, chemical control methods are commonly used to control Pseudostellaria chinensis soil-borne diseases. However, the use of chemical drugs to control soil-borne diseases is prone to cause pesticide residues, which in turn affects the safety and effectiveness of Pseudostellaria chinensis, a traditional Chinese medicine. Therefore, how to provide a safe and pollution-free method that can effectively inhibit Pseudostellaria chinensis soil-borne diseases and inhibit the continuous cropping problem of Pseudostellaria chinensis has become an urgent problem to be solved in this field. Summary of the Invention

[0004] In view of this, the present invention provides a microorganism that can inhibit soil-borne diseases of Pseudostellaria spp., inhibit continuous cropping obstacles of Pseudostellaria spp., and promote the growth of Pseudostellaria spp., belonging to the genus Burkholderia (Burkholderia cepacia), named SJ08 Burkholderia, which can effectively inhibit soil-borne diseases of Pseudostellaria spp. caused by Fusarium oxysporum, and specifically includes the following technical solutions:

[0005] The present invention provides a Burkholderia cepacia SJ08, which is preserved in the Guangdong Provincial Microbial Culture Collection Center with a preservation number of GDMCC No: 65908 and a preservation date of February 18, 2025.

[0006] The present invention also provides a microbial agent, comprising the Burkholderia SJ08 described above.

[0007] Preferably, the concentration of Burkholderia SJ08 in the microbial agent is ≥4×10 8 CFU / mL.

[0008] The present invention also provides a method for preparing the microbial agent as described above, comprising the following steps:

[0009] Burkholderia SJ08 was inoculated into LB liquid medium and cultured until the OD value of the contents of the LB liquid medium was 600 When the value reaches 0.5 to 0.6, the bacterial solution is obtained;

[0010] The bacterial solution was centrifuged for the first time, the supernatant was removed, sterile water was added, and the solution was centrifuged again, the supernatant was removed, and the precipitate was obtained;

[0011] Sterile water is added to the precipitate and mixed to obtain the microbial agent.

[0012] Preferably, the first centrifugation and the second centrifugation are both performed at 1000 rpm for 3 to 7 minutes.

[0013] The present invention also provides the use of the Burkholderia SJ08 strain, microbial agent, or the microbial agent prepared by the preparation method described above in any one or more of inhibiting soil-borne crop diseases, inhibiting continuous cropping obstacles, and promoting crop growth.

[0014] Preferably, the crops include medicinal crops; the medicinal crops include Pseudostellaria heterophylla.

[0015] Preferably, the soil-borne crop diseases include soil-borne diseases caused by Fusarium oxysporum.

[0016] The present invention also provides a method for inhibiting soil-borne crop diseases, inhibiting continuous cropping obstacles and / or promoting crop growth, which comprises soaking crop seeds with the microbial agent described above.

[0017] Preferably, the soaking time is 0.5 to 1.5 hours.

[0018] The beneficial effects of the present invention are:

[0019] The present invention provides a Burkholderia cepacia SJ08 strain, which is deposited with the Guangdong Provincial Microbial Culture Collection Center under the deposit number GDMCC No. 65908 and the deposit date of February 18, 2025. The Burkholderia cepacia SJ08 strain is isolated from the Pseudostellaria heterophylla variety and can effectively inhibit crop diseases caused by Fusarium oxysporum, prevent continuous cropping, and promote crop growth. It is safe and pollution-free, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments are briefly introduced below.

[0021] Figure 1 The comparison analysis results of the sequencing sequences in Example 1 of the present invention;

[0022] Figure 2 This is a schematic diagram of the results of the flat plate confrontation experiment in Example 2 of the present invention;

[0023] Figure 3 This is a schematic diagram of the experimental results of IAA production in Example 2 of the present invention;

[0024] Among them, the left side is the control group and the right side is the experimental group;

[0025] Figure 4 This is a schematic diagram of the ammonia production experimental results of Example 2 of the present invention;

[0026] Among them, the left side is the control group and the right side is the experimental group;

[0027] Figure 5 This is a schematic diagram of the potassium dissolution experimental results of Example 2 of the present invention;

[0028] Figure 6 This is a schematic diagram of the results of the phosphorus dissolution experiment in Example 2 of the present invention;

[0029] Figure 7 This is a schematic diagram of the gelatin liquefaction experimental results of Example 2 of the present invention;

[0030] The left side is the experimental group, and the right side is the control group;

[0031] Figure 8 The results of the antibacterial experiment on Burkholderia SJ08 in Example 5 of the present invention are shown below.

[0032] Among them, the left side is the Pseudostellaria baicalensis of the experimental group, and the right side is the Pseudostellaria baicalensis of the CK group.

[0033] Biological deposit information

[0034] Burkholderia SJ08, biological classification name: Burkholderia cepacia SJ08, was deposited in Guangdong Provincial Microbiological Culture Collection Center on February 18, 2025, with the deposit number GDMCCNo: 65908, and the deposit address is 100 meters west of No. 2, 102 Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province. DETAILED DESCRIPTION

[0035] The present invention provides a strain of Burkholderia cepacia SJ08, which is preserved in the Guangdong Provincial Microbial Culture Collection Center with a preservation number of GDMCC No: 65908, a preservation date of February 18, 2025, and a preservation location of the Guangdong Provincial Microbial Culture Collection Center. The Burkholderia SJ08 provided by the present invention is a flat, moist colony on LB solid culture medium, and the colony has a metallic luster. The Burkholderia SJ08 described in the present invention has the ability to produce IAA, ammonia, dissolve potassium, dissolve phosphorus, and dissolve gelatin. Moreover, whether it is a strain antagonism experiment or a Pseudostellaria spp. disease resistance experiment, Burkholderia SJ08 shows a good inhibitory effect on pathogenic Fusarium oxysporum. The Burkholderia SJ08 of the present invention can inhibit soil-borne diseases of Pseudostellaria spp. caused by pathogenic Fusarium oxysporum.

[0036] The present invention also provides a microbial agent, comprising the Burkholderia SJ08 described above.

[0037] As an embodiment, the concentration of Burkholderia SJ08 in the microbial agent of the present invention is ≥4×10 8 CFU / mL. As another embodiment, the concentration of Burkholderia SJ08 in the microbial agent of the present invention can be 4×10 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL and 9×10 8 In a specific embodiment, the concentration of Burkholderia SJ08 in the microbial agent of the present invention is 4×10 8 CFU / mL.

[0038] The present invention also provides a method for preparing the microbial agent as described above, comprising the following steps:

[0039] Burkholderia SJ08 was inoculated into LB liquid medium and cultured until the OD value of the contents of the LB liquid medium was 600 After the value reaches 0.5 to 0.6, a bacterial solution is obtained; the bacterial solution is centrifuged for the first time, the supernatant is removed, sterile water is added, and the solution is centrifuged again, the supernatant is removed, and a precipitate is obtained; sterile water is added to the precipitate, and the mixture is mixed to obtain the microbial agent.

[0040] The present invention inoculates Burkholderia SJ08 into LB liquid culture medium and cultures the contents of the LB liquid culture medium until the OD value is 600As an embodiment, the OD value of the contents of the LB liquid culture medium is 0.5 to 0.6, and the bacterial solution is obtained. 600 The value can be any one of 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59 and 0.6.

[0041] After obtaining the bacterial solution, the present invention performs a first centrifugation on the bacterial solution, removes the supernatant, adds sterile water, and centrifuges again to remove the supernatant to obtain a precipitate. In one embodiment, the first centrifugation and the second centrifugation are both performed at 1000 rpm for 3 to 7 minutes. In another embodiment, the duration of the first centrifugation and the second centrifugation can be any one of 3 minutes, 4 minutes, 5 minutes, 6 minutes, and 7 minutes. In one embodiment, the amount of sterile water added can be 0.5 to 1.5 mL. In another embodiment, the amount of sterile water added can be any one of 0.5 mL, 0.6 mL, 0.7 mL, 0.8 mL, 0.9 mL, 1.0 mL, 1.1 mL, 1.2 mL, 1.3 mL, 1.4 mL, and 1.5 mL. In the present invention, the bacterial solution is centrifuged at 1000 rpm for 5 minutes, performs a first centrifugation, removes the supernatant, adds 1 mL of sterile water, and centrifuges again at 1000 rpm for 5 minutes to remove the supernatant to obtain a precipitate.

[0042] After obtaining the precipitate, the present invention adds sterile water to the precipitate and mixes to obtain the microbial agent. As an embodiment, the effective concentration of the strain in the microbial agent is ≥4×10 8 CFU / mL. As another embodiment, the effective concentration of the microbial inoculant of the present invention can be 4×10 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL and 9×10 8 In a specific embodiment, the effective concentration of the microbial agent of the present invention is 4×10 8 CFU / mL.

[0043] The present invention also provides the use of the Burkholderia SJ08 strain, microbial agent or the microbial agent prepared by the preparation method described above in any one or more of inhibiting soil-borne crop diseases, inhibiting continuous cropping obstacles and promoting crop growth.

[0044] In one embodiment, the crops include medicinal crops, and the medicinal crops include Pseudostellaria heterophylla. In one embodiment, the soil-borne diseases of crops include soil-borne diseases caused by Fusarium oxysporum.

[0045] The present invention also provides a method for inhibiting soil-borne crop diseases, inhibiting continuous cropping problems, and / or promoting crop growth, comprising soaking crop seeds with the microbial agent described above. In one embodiment, the soaking time is 0.5 to 1.5 hours. In another embodiment, the soaking time can be any one of 0.5 hours, 0.6 hours, 0.7 hours, 0.8 hours, 0.9 hours, 1.0 hours, 1.1 hours, 1.2 hours, 1.3 hours, 1.4 hours, and 1.5 hours.

[0046] To further illustrate the present invention, the following detailed description of the Burkholderia SJ08 strain, bacterial agent, preparation method and application thereof is provided by the present invention in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0047] Example 1 Isolation, screening and identification of Burkholderia SJ08 strain

[0048] In this embodiment, Pseudostellaria chinensis seeds were collected from a Pseudostellaria chinensis planting area in Zherong County, Fujian Province. 5 g of Pseudostellaria chinensis seeds were weighed and placed in a conical flask. 45 mL of sterile water was added and the flask was placed in a constant temperature shaker and fully shaken for 30 min to obtain 10 -1 diluent.

[0049] Will be equipped with 10 -1 The conical flask of the dilution solution was placed in a 75℃ constant temperature water bath and heated for 30 min. 5 mL of 10 -1 The diluted solution was transferred to another conical flask containing 45 mL of sterile water to obtain 10 -2 According to the above operation, 10 -3 diluent, 10 -4 diluent.

[0050] Take 50 μL of dilutions of different concentrations and evenly spread them on LB culture medium, and then place them in a 37°C incubator for 12 hours. After the incubation, observe the growth of microorganisms at each dilution and select the 10 microorganisms with the best growth. -2 The dilution solution is used as the optimal dilution. All single colonies on the plate are selected and transferred to new plates for continued culture. The culture time is about 10 hours. When the colonies are clearly visible, the isolated and screened bacteria are obtained and placed in a 4°C refrigerator for short-term storage.

[0051] The isolated and screened bacteria were molecularly identified using PCR amplification of the 16s to 23s rRNA gene region. The primer sequences are shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequencing sequence is shown in SEQ ID NO.3:

[0052] 27F (SEQ ID NO. 1): 5′-AGAGTTTGATCCTGGCTCAG-3′;

[0053] 1492R (SEQ ID NO. 2): 5′-TACGACTTAACCCCAATCGC-3′;

[0054] SEQ ID NO.3:

[0055]

[0056] The PCR reaction system (25 μL) was as follows: 1 μL each of upstream and downstream primers, 2 μL of template, 8.5 μL of enzyme-free water, and 12.5 μL of mix.

[0057] The PCR reaction program was denaturation at 94°C for 1 min, annealing at 55°C for 45 s, and extension at 72°C for 90 s for 35 cycles. The sequencing sequence was compared with the NCBI database using the BLAST tool. The results are shown in the figure. Figure 1 shown.

[0058] Molecular biological identification determined that the bacterium was Burkholderia, named Burkholderia cepaciaSJ08. The isolated strain was deposited in the Guangdong Provincial Microbiological Culture Collection on February 18, 2025, with the deposit number GDMCCNo: 65908.

[0059] Observation of the Burkholderia SJ08 showed that the Burkholderia SJ08 was flat and moist colonies on the LB solid culture medium, and the colonies had a metallic luster.

[0060] Example 2 Experiment on the physiological and biochemical characteristics of Burkholderia SJ08

[0061] The screened Burkholderia SJ08 was transferred to LB liquid culture medium for expansion culture. The specific expansion culture was as follows: 30 μl of bacterial liquid was added to the sterilized LB liquid culture medium and cultured in a shaker to obtain Burkholderia SJ08 culture liquid, and plate confrontation test, IAA production experiment, ammonia production experiment, phosphate solubility experiment, potassium solubility experiment and gelatin liquefaction experiment were carried out respectively.

[0062] (1) Flat plate confrontation experiment

[0063] Use a sterilized white pipette tip to dip the pathogenic Fusarium oxysporum (Fusarium oxysporum was disclosed in the article "LiuYT, Zhang YX, Wang XY, et al. Pseudostellaria heterophylla cultivar mixtures driven changes in rhizosphere metabolites to suppress soil-borne Fusarium disease [J]. Agriculture Ecosystems & Environment, 2025, 380.") hyphae, spot it in the center of the culture dish filled with PDA culture medium, and place it in a 37°C biochemical incubator for 10 hours. Use a sterilized white pipette tip to dip Burkholderia SJ08, draw a 1 cm horizontal line at 1 cm from the center of the culture medium, and place it in a 37°C biochemical incubator for 24 hours. Observe the growth of Fusarium oxysporum and Burkholderia SJ08. The results are as follows: Figure 2 It can be seen that the Burkholderia SJ08 strain provided by the present invention can significantly inhibit the growth of pathogenic Fusarium hyphae.

[0064] (2) IAA production experiment

[0065] The Burkholderia SJ08 strain was inoculated into LB liquid medium and shaken to prepare a culture solution. Then 50 μl of the bacterial solution was inoculated into a liquid medium containing 3 mM L-tryptophan. The culture was shaken at 37° C. and 200 rpm for 24 h to obtain a Burkholderia SJ08 bacterial suspension.

[0066] Take 1mL of Burkholderia SJ08 suspension and centrifuge at 4000rpm / min and 4℃ for 10min. Take 500μl of supernatant and add it to a 2mL EP tube. Then add 1mL of Salkowski colorimetric solution (50mL 35% HClO4 + 1mL mol / L ferric chloride) to the EP tube. The solution in the EP tube is used as the experimental group, and the EP tube with pure 500μl culture supernatant added with 1mL of Salkowski colorimetric solution is used as the control. Place it in the dark at room temperature for 30min and observe the results. Figure 3 .

[0067] Depend on Figure 3 It can be seen that the solution in the control EP tube on the left did not change color, while the solution in the EP tube on the right to which the supernatant of the Burkholderia SJ08 bacterial suspension was added turned red, indicating that the Burkholderia SJ08 screened in this application can produce IAA.

[0068] (3) Ammonia production experiment

[0069] Ammonia production assay medium was prepared according to the composition shown in Table 1.

[0070] Table 1 Preparation of ammonia production assay medium

[0071]

[0072] 30 μl of Burkholderia SJ08 was inoculated into the ammonia production assay medium as the experimental group. At the same time, an ammonia production assay medium without inoculation was prepared as the control group. The control group and the experimental group were placed in a 37°C incubator for 24 hours. After incubation, 5 mL of sodium reagent was added to the culture medium and gently shaken. The results were as follows: Figure 4 As shown. Figure 4 It can be seen that the experimental group produced an orange-red precipitate, which was a positive reaction, indicating that Burkholderia SJ08 had the ability to produce ammonia.

[0073] (4) Potassium dissolution test

[0074] Prepare potassium-soluble medium according to Table 2.

[0075] Table 2 Preparation of potassium-soluble culture medium

[0076]

[0077] 30 μl of Burkholderia SJ08 was inoculated into potassium-soluble medium and cultured in a 37°C incubator for 48 h. Figure 5 As shown, according to Figure 5 It can be seen that a colorless transparent circle appears around Burkholderia SJ08, indicating that Burkholderia SJ08 has the ability to dissolve potassium.

[0078] (5) Phosphate dissolution experiment

[0079] Prepare phosphate solubilization medium according to Table 3.

[0080] Table 3 Configuration of phosphate solubilization medium

[0081]

[0082] Inoculate the test bacteria into the phosphate solubilizing medium, take 30μl of Burkholderia SJ08 bacterial solution and drop it in the center of the medium. After the bacterial solution dries, seal the plate and place it in a 37℃ incubator to observe whether there is a transparent circle around the bacteria. If a transparent circle appears, it proves that it has the ability to solubilize phosphate. Figure 6 ,Depend on Figure 6 It can be seen that the Burkholderia SJ08 strain screened out in the present invention has the ability to solubilize phosphate.

[0083] (6) Gelatin liquefaction experiment

[0084] Prepare gelatin liquefaction medium according to Table 4.

[0085] Table 4 Gelatin culture medium

[0086]

[0087] After the culture medium is prepared, sterilize it in a high-temperature and high-pressure autoclave (pressure 121MPa, time 21min). If the culture medium is in a solidified state before use, heat it to melt it and pour it into the plate. Use an inoculation loop to dip a small amount of SJ08 strain and inoculate it into the gelatin culture medium using the puncture inoculation method. After incubation at 37℃ for two days, place it in the refrigerator for a period of time, and check the liquefaction of the culture medium. The results are as follows: Figure 7 As shown, it can be seen that the culture medium of the experimental group is still in a liquefied state, indicating that the bacteria have the ability to liquefy gelatin.

[0088] Example 3 Preparation Method of Burkholderia SJ08 Bacterial Liquid

[0089] Burkholderia SJ08 was inoculated into 800 μL LB liquid and cultured for 18 h until OD 600 After the pH value was 0.5-0.6, a culture solution was obtained, which was centrifuged at 1000 rpm for 5 min, the supernatant was discarded, 1 mL of sterile water was added, and the mixture was shaken evenly. The supernatant was removed to wash away the residual LB, and 5 mL of sterile water was added to the precipitate and shaken evenly to prepare a bacterial solution. The concentration of Burkholderia SJ08 in the bacterial solution was 4.0 × 10 8 cfu / mL.

[0090] Example 4 A method for inhibiting soil-borne diseases and continuous cropping obstacles of Pseudostellaria heterophylla and promoting the growth of Pseudostellaria heterophylla

[0091] Pretreatment of seed ginseng: Select Pseudostellaria heterophylla seed ginseng of the same size, wash off the surface soil with clean water, disinfect the seed ginseng with 2% sodium hypochlorite solution for 1 minute, rinse with distilled water 5 times, then disinfect with 75% ethanol for 2 minutes, rinse with distilled water 3 times, and absorb the surface moisture with filter paper.

[0092] Soak the Pseudostellariae pseudoginseng seeds in the Burkholderia SJ08 bacterial solution as described in Example 2 for 30 minutes.

[0093] Example 5 Verification of the antibacterial effect of Burkholderia SJ08

[0094] In this example, 12 Pseudostellaria heterophylla species were taken and divided into 4 groups on average.

[0095] Treatment 1: Take 3 Pseudostellaria heterophylla seeds and soak them in the Burkholderia SJ08 bacterial solution prepared by the preparation method described in Example 3. After soaking for 30 minutes, take out the seeds;

[0096] Treatment 2 (CK group): 3 ginseng seeds were soaked in sterile water as CK control, and the ginseng seeds were taken out after soaking for 30 minutes;

[0097] Treatment 3: Three Pseudostellaria chinensis seeds were soaked in the SJ06 inoculant for 30 minutes before removal. The SJ06 inoculant was prepared using the same method as in Example 3, except that the strain SJ06 was used. SJ06 is another strain that the applicants screened from Pseudostellaria chinensis seeds in previous experiments using the method described in Example 1.

[0098] Treatment 4: Take three Pseudostellariae seeds and soak them in the FJ11 inoculant for 30 minutes before removing the seeds. The preparation method for the FJ11 inoculant is the same as in Example 3, except that the FJ11 strain is used. FJ11 is another strain that the applicant screened from Pseudostellariae seeds in previous experiments using the method described in Example 1.

[0099] Use a scalpel in a sterilizing pot to cut a 0.5 cm small incision on the surface of the ginseng seeds soaked in the above different treatments. Use a sterilized white gun tip to dip the mycelium of Fusarium oxysporum and apply it to the wound surface. Place the ginseng seeds in a culture dish covered with sterilized filter paper, then add 4 mL of sterile water to the culture dish to keep the ginseng seeds moist. Place the culture dish in a biochemical incubator and culture at 37°C. Observe the growth of Fusarium oxysporum on the wound surface of the ginseng seeds. The results are as follows. Figure 8 The lengths of the colonies of Pseudostellaria heterophylla infected by Fusarium oxysporum at 24, 36 and 48 h after inoculation were counted, and the results are shown in Table 5.

[0100] Table 5 Length of Fusarium oxysporum fungus at different times (cm)

[0101] Treatment group / time (h) 24 36 48 Process 2 (CK) 0.5 0.8 1.23 Process 1 0.32 0.45 0.62 Process 3 0.48 0.73 1.05 Process 4 0.48 0.75 1.14

[0102] Depend on Figure 8 As shown in Table 5, compared with the control group, after the ginseng treated with Burkholderia SJ08 was infected by pathogenic Fusarium oxysporum, the wound surface formed by the wound deformation was smaller and the length of the Fusarium oxysporum fungus block was smaller, indicating that the bacteria has a good antibacterial effect.

[0103] In summary, the present invention provides a Burkholderia SJ08. The bacterial liquid prepared using the Burkholderia SJ08 described in the present invention has a good inhibitory effect on soil-borne diseases of Pseudostellaria zeylanicum, can effectively inhibit crop diseases caused by Fusarium oxysporum, and can also inhibit crop continuous cropping disorders and promote crop growth. It is safe and pollution-free, and has good application prospects.

[0104] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments like this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A Burkholderia cepacia SJ08, characterized in that: The Burkholderia SJ08 is deposited in Guangdong Provincial Microbiological Culture Collection Center with the deposit number GDMCC No: 65908 and the deposit date of February 18, 2025.

2. A microbial agent, characterized in that: The active ingredient in the microbial agent includes the Burkholderia SJ08 as described in claim 1.

3. The microbial agent according to claim 2, wherein The concentration of Burkholderia SJ08 in the microbial agent is ≥4×10 8 CFU / mL.

4. The method for preparing the microbial agent according to claim 2 or 3, characterized in that: The steps include: Burkholderia SJ08 was inoculated into LB liquid medium and cultured until the OD value of the contents of the LB liquid medium was 600 When the value reaches 0.5 to 0.6, the bacterial solution is obtained; The bacterial solution was centrifuged for the first time, the supernatant was removed, sterile water was added, and the solution was centrifuged again, the supernatant was removed, and the precipitate was obtained; Sterile water is added to the precipitate and mixed to obtain the microbial agent.

5. The preparation method according to claim 4, wherein The specific operations of the first centrifugation and the second centrifugation are both centrifugation at 1000 rpm for 3 to 7 minutes.

6. Use of the Burkholderia SJ08 according to claim 1, the microbial agent according to claim 2 or 3, or the microbial agent prepared by the preparation method according to claim 4 or 5 in any one or more of suppressing soil-borne crop diseases, suppressing continuous cropping disorders, and promoting crop growth.

7. The use according to claim 6, characterized in that The crops include medicinal crops; the medicinal crops include Pseudostellaria heterophylla.

8. The use according to claim 6, characterized in that The soil-borne diseases of crops include soil-borne diseases caused by Fusarium oxysporum.

9. A method for inhibiting soil-borne crop diseases, inhibiting continuous cropping disorders and / or promoting crop growth, characterized in that: Crop seeds are soaked with the microbial agent as claimed in claim 2 or 3.

10. The use according to claim 9, characterized in that The soaking time is 0.5 to 1.5 hours.

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