Composition with sensitive skin repairing and anti-aging functions as well as emulsion and application thereof

Abstract

The invention discloses a composition with sensitive skin repairing and anti-aging functions as well as an emulsion and application of the composition, and belongs to the technical field of cosmetics. The composition specifically comprises a pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5, acetylchitosamine, sodium hyaluronate, silanetriol, caffeine and acetyl hexapeptide-8, and can promote the generation of laminin-5 and integrin beta1, promote the vitality of keratinocytes and fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, inhibit the growth of the keratinocytes and the fibroblasts, and promote the growth of the keratinocytes and the fibroblasts. The generation of inflammatory factors (IL-6) is inhibited; the generation of collagen IV and collagen VII is promoted; the invention further provides an emulsion prepared from the composition, and the optimal formula of the emulsion is obtained through optimization. The active composition disclosed by the invention has a wide application prospect in the field of cosmetics, and particularly has important significance in sensitive skin repair and anti-aging directions.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition having sensitive skin repair and anti-aging functions, an emulsion thereof, and applications thereof. Background Art

[0002] In recent years, the proportion of people with sensitive skin has been increasing. With the widespread adoption of scientific skincare and the continuous advancement of industry technology, people with sensitive skin have gradually advanced from basic care to functional skincare, with anti-aging needs becoming increasingly prominent. Sensitive skin can present problems such as a damaged skin barrier, neurovascular hyperreactivity, and skin inflammation. Furthermore, skin tolerance is poor, making it less able to resist external stimuli and more sensitive to UV rays that cause photoaging. It is also more susceptible to aging issues such as dry lines, fine lines, and decreased skin elasticity. Typical skincare strategies for sensitive skin focus on barrier repair, moisturizing, and anti-inflammatory measures. Due to the complex causes of sensitive skin, symptoms can recur, and there can be intolerance to functional ingredients and products. For people with sensitive skin, improving skin sensitivity, maintaining skin health and stability, and meeting the skin's anti-aging needs are difficult skincare pain points to address. Patent CN 119157771 A discloses a polypeptide composition with repairing and anti-aging effects, as well as its preparation method and application. The composition composed of polypeptide and milk thistle can enhance fibroblast activity and promote collagen and keratinocyte repair, but there are no reports on its use in improving the skin of people with sensitive skin.

[0003] The amount of collagen in the skin directly determines the thickness and elasticity of the skin. In addition to the quantity, the quality of collagen also has an important impact on its role in the skin barrier function. Among them, type I collagen is related to the elasticity of the skin; types IV and VII and tight proteins are related to the firmness of the skin, communication between the true epidermis and the epidermis, and wound healing. At the same time, collagen can promote the proliferation of collagen-forming fibroblasts, optimize the extracellular matrix microenvironment, and induce cell self-repair, thereby endogenously promoting the growth and repair of skin tissue, and further promoting barrier recovery and inflammation regulation. Therefore, promoting the various collagen structures of the skin and enhancing the vitality of epidermal and dermal cells, promoting skin health, improving sensitive conditions and skin aging is a comprehensive and relatively safe solution for anti-aging of sensitive skin. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition having sensitive skin repair and anti-aging functions, an emulsion thereof and an application thereof.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: The present invention provides a composition with sensitive skin repair and anti-aging functions, comprising the following components: Component A: Pseudoalteromonas ferment extract, collagen and palmitoyl tripeptide-5; Component B: acetyl glucosamine, sodium hyaluronate and silanetriol; Component C: Caffeine, Acetyl Hexapeptide-8.

[0006] Furthermore, the composition includes the following components, in parts by weight: Pseudoalteromonas fermentation extract: 0.15-5 parts; Collagen: 0.003-0.05 parts; Palmitoyl tripeptide-5: 0.0001-0.1 parts; Acetyl glucosamine: 0.05-3 parts; Sodium hyaluronate: 0.1018-0.135 parts; Silanetriol: 0.0015-0.03 parts; Caffeine: 0.1-0.5 parts; Acetyl hexapeptide-8: 0.0002-0.1 parts.

[0007] Furthermore, the components A and B have the ability to synergistically promote cell proliferation and type I collagen production; the composition A promotes the activation of transforming growth factor (TGF-β).

[0008] Furthermore, the composition A, composition B and composition C synergistically promote the production of type IV and VII collagen.

[0009] Furthermore, the composition A, composition B and composition C promote the production of multilaminin-5 and integrin β1.

[0010] Furthermore, the composition includes the following components, in parts by weight: 0.25 parts of Pseudoalteromonas fermentation product extract, 0.005 parts of collagen, 0.0005 parts of palmitoyl tripeptide-5, 1 part of acetyl glucosamine, 0.107 parts of sodium hyaluronate, 0.006 parts of silanetriol, 0.5 parts of caffeine, and 0.001 parts of acetyl hexapeptide-8.

[0011] The present invention also provides an emulsion prepared by using the composition, wherein the 40°C ΔBS value of the emulsion is 0.30%-5%; and the -20°C ΔBS value of the emulsion is 2%-5%.

[0012] Furthermore, the particle size of the emulsion is 750-790 nm.

[0013] Furthermore, the preparation method of the emulsion comprises the following specific steps: (1) Preparation of oil phase: Weigh the oil and emulsifier in the oil phase, heat in a water bath, stir and keep warm until they are completely dissolved into the oil phase; (2) Preparation of thickening phase: Weigh the thickener, mix it with the humectant, add water, heat and swell it to form a thickening phase; (3) Mix the oil phase and thickening phase homogeneously, stir and cool; (4) adding the composition, moisturizer and preservative, and stirring to obtain the emulsion.

[0014] Furthermore, the oil includes at least one of isononyl isononanoate, shea butter and polydimethylsiloxane; the emulsifier includes at least one of SIMULSOL165, C12-20 acid PEG-8 ester and cetyl alcohol; the thickener includes at least one of ammonium acryloyldimethyltaurate / VP copolymer, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer and xanthan gum; the moisturizer includes at least one of tocopheryl acetate and sorbitol.

[0015] Furthermore, in step (1), the water bath heating temperature is 80-85° C., and the insulation time is 20-45 min.

[0016] Furthermore, the heating temperature in step (2) is 80-85°C.

[0017] Furthermore, the thickening phase in step (2) is in a translucent, uniform, non-flowable gel state.

[0018] Furthermore, the homogenization speed in step (3) is 2900-3000 rpm, the homogenization time is 5-10 min, and the temperature after cooling is 35-45°C.

[0019] Furthermore, the stirring time in step (4) is 4-8 minutes.

[0020] Furthermore, in parts by weight, the amount of the oil is 0-8 parts of isononyl isononanoate, 0-6 parts of avocado butter, and 1 part of polydimethylsiloxane; the amount of the emulsifier is 0-0.8 parts of SIMULSOL165, 0-2.5 parts of C12-20 acid PEG-8 ester, and 0-2 parts of cetyl alcohol; the amount of the thickener is 0-0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer, 0-0.15 parts of acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, and 0-0.1 parts of xanthan gum; the amount of the moisturizer is 0-2 parts of tocopheryl acetate and 0-2 parts of sorbitol.

[0021] Furthermore, the optimal formula of the emulsion is, in parts by mass: 4 parts of isononyl isononanoate; 3 parts of shea butter; 1 part of polydimethylsiloxane; 1 part of tocopheryl acetate; 2 parts of cetyl alcohol; 0.8 parts of SIMULSOL 165; 2.5 parts of C12-20 acid PEG-8 ester; 0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer; 0.05 parts of xanthan gum; 1 part of sorbitol; and 0.15 parts of acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer.

[0022] The present invention also provides use of the composition or the emulsion in preparing cosmetics with sensitive skin repair and anti-aging functions.

[0023] Compared with the prior art, the present invention has the following advantages and beneficial effects 1. The present invention provides a composition that acts on the collagen and tight protein structure of the entire skin layer, has both the effects of repairing sensitive skin and anti-aging. The composition can promote the production of type I, type IV and type VII collagen, as well as laminin-5 and integrin β1 in all layers of the skin, promote the vitality of keratinocytes and fibroblasts, inhibit the production of inflammatory factors (IL-6), and improve skin aging.

[0024] 2. Component A of the present invention can endogenously promote the production of collagen and tight proteins in all layers of the skin and promote cell vitality; component B can promote the signal transduction of hyaluronic acid, endogenously promote the synthesis of hyaluronic acid and the secretion and synthesis of EGF, participate in the communication between cells and ECM, strengthen the communication between cells, inhibit inflammatory factors, and provide nutritional and environmental support for collagen production and barrier repair; component C can improve the appearance of aging skin, reduce wrinkles and enhance firmness.

[0025] 3. The present invention provides a method for preparing an emulsion using the above composition. The optimal ratio of the emulsion is obtained through experimental optimization. At the same time, the obtained emulsion has a 40°C ΔBS value of 0.30%-5%, and the particle size of the emulsion is 750-790nm.

[0026] 4. The composition of the present invention can improve skin elasticity and firmness, reduce wrinkles, enhance skin fineness and smoothness, and can be used in anti-aging care products for sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Added case images of the collagen echo in the dermis of the skin after using the lotion of Experimental Example 2 for 2 and 4 weeks.

[0028] Figure 2 This is an example of skin redness improvement after using the lotion in Experimental Example 2 for 4 weeks.

[0029] Figure 3This is an example of how skin wrinkles and fineness improved after using the lotion in Experimental Example 2 for 4 weeks. DETAILED DESCRIPTION

[0030] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the reagents, materials, instruments, etc. used in the following examples are all commercially available unless otherwise specified.

[0031] The Pseudoalteromonas fermentation product extract in the present invention is sourced from LIPOTEC SAU; collagen and palmitoyl tripeptide-5 are sourced from Shanghai Jiakai Biotechnology Co., Ltd.; acetyl hexapeptide-8 is sourced from LIPOTEC SAU; caffeine is sourced from AXIALYS INNOVATIONS, acetyl glucosamine is sourced from Bloomage Biotechnology Co., Ltd., and sodium hyaluronate and silanetriol are sourced from EXSYMOL SAM, EXSYMOL SAM, and Bloomage Biotechnology Co., Ltd. SIMULSOL 165 was sourced from SEPPIC SA; C12-20 PEG-8 esters were sourced from KREGLINGEREUROPE NV, and cetyl alcohol was sourced from Edenor Oleochemicals (M) Sdn. Bhd.; ammonium acryloyldimethyltaurate / VP copolymer was sourced from Clariant Chemical Technology (Shanghai) Co., Ltd.; acrylates / C10-30 alkyl acrylate crosspolymer was sourced from Evonik Operations GmbH; xanthan gum was sourced from CP KelcoSingapore Pte., Ltd.; isononyl isononanoate, shea butter, and dimethicone were sourced from The Nisshin OilliO Group, Ltd., Jarchem Innovative Ingredients, LLC, and The Dow Chemical (Shanghai) Co., Ltd., respectively; and tocopheryl acetate was sourced from DSM Nutritional Products. Ltd.; sorbitol was sourced from Roquette (China) Fine Chemicals Co., Ltd.; 1,2-pentanediol was sourced from Dezhixin Flavors & Fragrances (Nantong) Co., Ltd.; and 1,3-butanediol was sourced from KH Neochem Co., Ltd.

[0032] Example 1: Screening experiment of active components 1. Active ingredient mouse fibroblast proliferation experiment Prepare different active ingredient combination solutions according to the mass of different active substances in every 100g aqueous solution. Measured by content (g).

[0033] Active component A includes Pseudoalteromonas fermentation extract, collagen, and palmitoyl tripeptide-5; active component B includes acetyl glucosamine, sodium hyaluronate, and silanetriol; and active component C includes acetyl hexapeptide-8 and caffeine. Compositions 1-9 combine components A, B, and C at different concentrations according to orthogonal experimental rules, while compositions 10-18 combine high, medium, and low concentrations of each combination. Specific contents are shown in Table 1.

[0034] Table 1. Content of each active ingredient

[0035] The experimental materials for the cell culture were DMEM high-glucose medium, PBS (pH = 7.4) buffer, FBS, 0.25% trypsin, MTT, and DMSO. Negative control: complete culture; positive control: 20 ng / mL transforming growth factor β1 (TGF-β1). Well-grown L929 mouse fibroblasts were cultured at 2×10 cells per well. 4 Cells were seeded at a density of 100 μL / mL in a 96-well culture plate, with 100 μL per well inoculated. The plates were incubated overnight at 37°C in a 5% CO2 environment. The culture medium was discarded, and samples of varying concentrations were added and diluted with complete DMEM culture medium. Six replicates were set up for each concentration. Complete DMEM culture medium was added to the cell control group, and complete culture medium containing 20 ng / mL transforming growth factor β1 (TGF-β1) was added to the positive control group, with 100 μL per well inoculated. The plates were incubated for 48-72 hours. The culture medium was discarded, the plates were washed twice with PBS, and 100 μL of MTT (1.0 g·L) was added. -1 ) solution, incubate at 37°C, 5% CO2 for 4 hours, discard the solution, add 150μL DMSO, and incubate at 37°C for 10 minutes. Then measure the absorbance of each well at a wavelength of 490nm.

[0036] Calculation formula: V(%)=(OD 样品 -OD 对照 ) / (OD 细胞对照 -OD 空白对照 ) × 100% In the formula: V%=(OD−OD) / (OD−OD)×100% V(%)——cell viability,; OD sample - the absorbance of the reaction system containing the sample to be tested; OD blank control - absorbance of an empty plate without any substance; OD cell control - absorbance of the reaction system without the sample to be tested; The components and proportions of each composition in 100 g of aqueous solution are shown in Table 1, and the cell proliferation test results of each composition are shown in Table 2.

[0037] Table 2. Experimental results of each active composition promoting fibroblast proliferation

[0038] 2. ELISA method to detect type Ⅰ collagen content Based on the results of the cell proliferation assay, active compositions 1, 2, 4, 5, and 11 were assayed for type I collagen content. Cells were stably passaged twice from cryopreserved cells and seeded into 96-well plates. The cells were cultured overnight in a 37°C, 5% CO2 incubator. The culture medium in the 96-well plates was discarded, and blank, positive, and test sample groups (1%, 0.5%, and 0.1%) were set up for dosing. Three parallel groups were set up for each group. After dosing, the 96-well plates were placed in a CO2 incubator and incubated for 24 h ± 2 h. After incubation, the samples were assayed using the human type I collagen enzyme-linked immunosorbent assay kit (ELISA) with the absorbance of each well measured at 450 nm using a microplate reader. A corresponding curve was constructed, with the absorbance (OD) value as the abscissa (X) and the corresponding concentration of the test substance standard as the ordinate (Y). The test substance content in the sample was calculated from the standard curve based on the OD value. The final type I collagen result was calculated as the average of the three replicate wells in each group.

[0039] Calculation formula for type I collagen upregulation rate Increase rate (%) = (T / C-1) × 100% Where: T is the average content of type I collagen in the test substance; C is the average content of type I collagen in the blank control.

[0040] The measurement results are shown in Table 3.

[0041] Table 3. The upregulation rate of type I collagen by each active composition

[0042] According to the above experimental results, excessively high concentrations of acetyl glucosamine, caffeine and Pseudoalteromonas fermentation product extract have certain cytotoxicity. At medium and low concentrations, component A (Pseudoalteromonas fermentation product extract, collagen, palmitoyl tripeptide-5) and component B (acetyl glucosamine, sodium hyaluronate and silanetriol) have the effect of synergistically promoting cell proliferation and promoting the production of type I collagen.

[0043] 3. Human skin keratinocyte protein expression test (multi-layer adhesion protein-5, integrin β1, COL IV, COL VII) Based on the results of cell proliferation and type I collagen content experiments, active ingredient combinations 1, 2, 4, and 5 were tested for protein expression in human keratinocytes. Human keratinocyte NHEK cells were used. This cell line has passed cell line quality control testing and STR genotyping, and the results were satisfactory. The number of passages in this experiment was P 14. Reagents included fetal bovine serum, high-glucose DMEM medium, penicillin-streptomycin solution, trypsin-EDTA solution, human type IV collagen (COL IV) kit, human type VII collagen (Col VII) kit, human laminin-5 (LM 5) kit, human integrin β1 (ITG β1) kit, and TGF-β1. Instruments included an inverted microscope, a CO2 incubator, and a microplate reader.

[0044] Experimental setup: ① Blank control: complete culture medium; ② Positive control: 100 ng / mL TGF-β1; ③ Different composition and comparative sample concentrations: 1%, 0.5%, and 0.1%. Cell preparation: Cell cultures from cryopreserved cells were stably passaged twice and seeded into 24-well plates. Three replicates were set up for each of the blank, positive, and test sample groups. The plates were incubated overnight in a CO2 incubator. Approximately 24 hours after the cells formed a monolayer, the culture medium was discarded from the 24-well plates and the sample solutions of varying concentrations were added. After dosing, the plates were incubated in a CO2 incubator for 48 hours. Protein Assays: After incubation, cell supernatants were collected and assayed using the human collagen type IV (COLIV) kit, human collagen type VII (Col VII) kit, human laminin-5 (LM 5) kit, and human integrin β1 (ITG β1) kit. The absorbance of each sample well was measured at 450 nm using a microplate reader. The viability of human skin keratinocyte NHEKs is shown in Table 4, and the results of the various protein assays are shown in Table 5.

[0045] Table 4. Experimental results of each active composition promoting the proliferation of human skin keratinocytes NHEK

[0046] Table 5. The upregulation rates of multilaminin-5, integrin β1, COL IV, and COL VII promoted by each active composition

[0047] 4. Inhibition of IL-6 secretion by RAW264.7 mouse macrophages Based on the results of human skin keratinocyte protein expression tests, Compositions 1 and 2 were used in an experiment to inhibit the secretion of the inflammatory factor IL-6 by RAW264.7 mouse macrophages to determine their anti-inflammatory effects. Cryopreserved mouse mononuclear macrophages (RAW264.7) were revived and cultured in a T25 culture flask with 5 mL of complete culture medium (DMEM high-glucose medium, 10% FBS). The culture medium was then replaced with 5 mL of complete culture medium (DMEM high-glucose medium, 10% FBS) in an incubator at 37°C and 5% CO2 for 24 hours, and the culture medium was then replaced for passage.

[0048] RAW264.7 cells cultured to the logarithmic growth phase were plated at 1×10 5 The cells were seeded at a density of 100 cells / well in a 96-well plate. Except for the cells in the normal group, the cells in the other groups were added with a final concentration of 1µg∙mL -1 After 24 hours of culture, cells were divided into the following groups and incubated for an additional 24 hours: NC group (normal group), 200 µL of complete culture medium was added; MC group (model group), 200 µL of complete culture medium containing 1 µg∙mL-1 LPS was added; SL group (low-dose group), 200 µL of complete culture medium containing 1 µg∙mL-1 LPS was added, with a sample concentration of 0.1%; SM group (medium-dose group), 200 µL of complete culture medium containing 1 µg∙mL-1 LPS was added, with a sample concentration of 0.5%; SH group (high-dose group), 200 µL of complete culture medium containing 1 µg∙mL-1 LPS was added, with a sample concentration of 1%. After 24 hours of culture, cell supernatants were collected and IL-6 levels were measured using an ELISA kit. The results of the inhibition of inflammatory factors by each combination are shown in Table 6.

[0049] Table 6. Inhibition of IL-6, an inflammatory factor in macrophages of RAW264.7 mice, by each active composition

[0050] According to experimental results, the palmitoyl tripeptide-5 in component A (Pseudoalteromonas fermentation extract, collagen, and palmitoyl tripeptide-5) of the present invention can promote the activation of transforming growth factor (TGF-β), significantly enhancing cell activity and promoting collagen synthesis. Collagen, a major component of the dermal extracellular matrix and a structural protein, provides a source substrate for collagen synthesis. The silanol in component B (acetylglucosamine, sodium hyaluronate, and silanetriol) enhances the permeability of hyaluronic acid and acetylglucosamine, improving the cellular microenvironment and providing nutrients for cell growth and collagen synthesis. Components A, B, and C synergistically provide essential structural support for keratinocytes and fibroblasts, regulate inflammation, promote epidermal renewal and skin repair, and promote and control the exchange of substances between the dermis and the epidermis.

[0051] Components A, B, and C of the present invention can also effectively promote the production of type IV and VII collagen. Type IV collagen can promote the proliferation of keratinocytes, increase epidermal thickness, and improve basement membrane structure, thereby enhancing the skin's barrier function. Type VII collagen plays an important role in maintaining skin strength and elasticity and promoting wound healing.

[0052] Components A, B, and C of the present invention can also effectively promote the production of laminin-5 and integrin β1. Laminin-5 is a major component of the epithelial basement membrane and plays an important role in the stable binding between epithelial cells and the basement membrane. It mediates cell adhesion to the extracellular matrix, helping cells maintain their correct position and is crucial for the structure of skin tissue and the integrity of the barrier. Integrin β1 participates in skin tissue remodeling, regulating the migration and proliferation of fibroblasts, causing them to secrete appropriate amounts of collagen and other extracellular matrix components, helping to repair damaged skin tissue and restore the skin's normal structure and function. It also interacts with intracellular signaling pathways to influence the expression of inflammation-related genes, thereby regulating skin inflammatory responses.

[0053] According to the screening results of different cell models, active composition 2 was comprehensively obtained as the best combination, which was further prepared into an emulsion.

[0054] Example 2: Emulsion formula screening experiment In this example, active ingredient combination 2 obtained in Example 1 was used to formulate an emulsion comprising an emulsifier, a thickener, an oil, a preservative, a humectant, and water. The emulsifiers included at least SIMULSOL 165, C12-20 PEG-8 acid ester, and cetyl alcohol; the thickeners included at least ammonium acryloyldimethyltaurate / VP copolymer, acrylates / C10-30 alkyl acrylate crosspolymer, and xanthan gum; the oils included isononyl isononanoate, Butyrospermum parkii (shea) butter, and dimethicone; and the humectants included tocopheryl acetate and sorbitol.

[0055] 1. Preparation of Emulsion Based on the results of the efficacy composition screening experiment, Composition 2 was further prepared into an emulsion. The mass and amount of the formulation parameters of each experimental example, calculated based on 100g of emulsion, are shown in Table 7. The specific preparation method is as follows.

[0056] (1) Preparation of oil phase: Weigh the oil and emulsifier in the oil phase, heat in a water bath to 80-85°C, stir, and keep warm for 30 minutes. Observe the state of the oil phase. If it is clear and transparent without particles, it is completely dissolved. (2) Preparation of thickening phase: Weigh different thickening materials of ammonium acryloyldimethyltaurate / VP copolymer, acrylic acid (ester) / C10-30 alkyl acrylate cross-polymer and xanthan gum, mix them with 1,3-butanediol, sorbitol and 1,2-pentanediol, add water, heat and stir at 80-85°C to swell, and form a thickening phase; the thickening phase is a translucent, uniform, non-flowable gel state; (3) After mixing the heated and dissolved oil phase with the thickening phase, homogenize at a high speed of 2900-3000 rpm for 5-10 minutes; then stir and cool to below 40°C; (4) Add the active ingredient combination solution and preservatives, and stir at medium-low speed for 5 minutes to obtain a combination emulsion.

[0057] Table 7. Recipe parameters for each experimental example ,

[0058] Example 3: Investigation of the physicochemical properties and stability of the emulsion Sample stability was tested using a Turbiscan Lab multiple light scattering (MLS) analyzer (Formulaction, France). The instrument's probe consists of a pulsed near-infrared light source (wavelength 880 nm) and two synchronized detectors. The probe moves vertically across the sample cell, within a range of 0-55 mm, scanning the entire sample. Transmitted and backscattered light data are collected at 40 μm intervals. The resulting graphs demonstrate the uniformity of sample concentration and particle size. The ΔBS values measured over 24 hours under heat-resistant conditions (40°C) and freeze-thaw conditions (-20°C) are used as test results. Generally, a ΔBS value of 0 < 5% indicates absolute stability, 5% < 8% indicates relative stability, and ΔBS > 8% indicates instability. The test results for each experimental example are shown in Table 8.

[0059] Table 8 Stability test results of each experimental example

[0060] Stability testing results indicate that the emulsion's stability is related to the synergistic effect of the emulsifiers SIMULSOL 165 and C12-20 PEG-8 esters. Furthermore, cetyl alcohol, as a co-emulsifier, synergistically enhances the formula's stability. Regarding oils and fats, high levels of BUTYROSPERMUM PARKII butter and isononyl isononanoate can affect the emulsifier's emulsification, thereby impacting the emulsion's final stability. In addition to the emulsifiers and oils, the thickeners ammonium acryloyldimethyltaurate / VP copolymer and acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer are combined to maintain the emulsion's viscosity after the active ingredients are added to the system, enhancing its low-temperature stability.

[0061] Example 4: Emulsion particle size testing and sensory evaluation Based on the stability test results, particle size measurement and sensory evaluation were performed on Experiments 1, 2, 4, and 5, which showed high stability. Measurements were performed using a Malvern particle size analyzer. Parameter settings included: Temperature: 22°C, Duration Used: 60, Count Rate (kcps): 485.9, Measurement Position (mm): 4.65, Cell Description: Disposablesizing cuvette, Attenuator: 8. Click the "Start" button. After the measurement is complete, a trend graph and statistical data, such as average particle size and particle size distribution, will be displayed. The software can then select a record to record the emulsion particle size.

[0062] The application process was assessed using a sensory testing method for smear-type products. The sensory evaluation items were divided into stickiness, moisturizing, and absorption speed. Eleven subjects were recruited and recorded sensory evaluation scores during the application process. The scores were assigned on a 7-point scale, with 1 for stickiness (least sticky, 7 for most sticky), and so on. Moisturizing (1 for least moisturizing, 7 for most moisturizing), and so on. Absorption (1 for slowest absorption, 7 for best absorption, and so on) was assessed. The particle size measurement results and sensory evaluation results are shown in Table 9.

[0063] Table 9 Emulsion particle size test results and sensory test scores of each experimental example

[0064] In terms of emulsion particle size, the combination of emulsifier SIMULSOL 165 and C12-20 acid PEG-8 ester helps optimize the emulsion's particle size and maintain a small size under current high-speed homogenization process conditions. The emulsion's particle size and the oil / fat ratio in the formula jointly influence its absorption and moisturizing properties. Particle size results and sensory evaluations show that Experiment 2 exhibits a smaller particle size and a higher skin feel score. Furthermore, the addition of BUTYROSPERMUM PARKII fruit butter synergizes with the relatively refreshing isononyl isononanoate, maintaining stability and a small particle size under current emulsification conditions and process parameters, while also providing superior absorption and enhancing the emulsion's moisturizing properties.

[0065] Example 5: Human efficacy evaluation of emulsion Based on the above test results, the emulsion prepared in Experimental Example 2 was subjected to a human efficacy evaluation test.

[0066] 1. Test conditions Test environment temperature: (21±1)°C; relative humidity: 40%-60%. Avoid measurements in direct sunlight. Maintain consistent test conditions throughout the test, including lighting conditions, instrument parameter settings, image acquisition and positioning method, image analysis software version and parameter settings, test area, and tester capabilities. Subjects must remain stable under these conditions for at least 30 minutes before evaluation and testing. For a series of tests, maintain consistent conditions as much as possible.

[0067] 2. Subject requirements Eleven eligible subjects were selected. Eligible subjects met the following criteria: age 35-60 years, a self-assessed score of 30 or higher on the Bergman Skin Type Questionnaire (Sensitive Skin), self-reported sensitive symptoms such as facial erythema, dryness, and itching, and significant facial wrinkles, dull skin tone, and sagging. Subjects were required to use the product regularly and as directed throughout the study period, and to refrain from using other products. Subjects were ineligible for inclusion if: pregnant, breastfeeding, or attempting pregnancy; currently participating in a similar study; recently undergone facial treatment, cosmetic surgery, or surgery; or had severe chronic medical conditions, endocrine disorders, major infectious diseases, or facial skin diseases that affect skin physiology or color changes, potentially impacting assessment. Other subjects deemed unsuitable by the trial investigators.

[0068] 3. Test items and steps During the subject's first visit, the study was explained to the subject. After being fully informed, the subject signed a written informed consent form. Subjects were asked questions based on the inclusion and exclusion criteria, and a comprehensive assessment of the subject's skin area and test screening records were performed. Test and control samples were distributed, and the subject was informed of their use. For 30 minutes before each round of testing, the subject was asked to relax. The test area should be exposed and relaxed. The subject should cleanse their face with water, allow it to air dry, and then wait for testing. After receiving the samples, the subject underwent a four-week test. Each morning and evening, the subject should apply a soybean-sized amount of the test sample evenly to both sides of their face, replacing their own serum and lotion. Facial skin examinations were performed before the test, and in weeks 1, 2, and 4. VISIA-CR images were taken of the subject's front, left, and right sides of the face during each round. Data from the subject was collected using a Dermalab skin tester during each round. Transepidermal water loss, elasticity data, and collagen density images were collected at fixed points on both sides of the subject's face. Stratum corneum moisture and color were tested at fixed points on both sides of the subject's face, forehead, and chin. Each point was tested 3 times and the average value was taken. The data and images were recorded and statistically analyzed.

[0069] 4. Test Results In human efficacy evaluation, elasticity values (E) and (VE) represent changes in skin elasticity and improvements in aging. Young's modulus (E) is calculated by measuring the distance the skin stretches within the probe chamber when a specific, preset negative pressure is applied. The shorter the stretch, the higher the E value, indicating greater skin rigidity. The viscoelastic (VE) value is calculated by dividing the retraction time by the elastic modulus, taking into account both the lifting and retraction phases. Higher VE values indicate greater skin elasticity.

[0070] According to the test results, combined with Table 10, after using the lotion sample for 4 weeks, the E value and VE value increased by 6.65% and 8.26% respectively. The skin ultrasound probe can detect the density of the dermis and the content of skin collagen. After using the lotion sample for 4 weeks, the density of the dermis increased by 15.94%. Figure 1It shows that the bright yellow area in the photo is the echo color of collagen, and the increase in the bright yellow area indicates an increase in the collagen content in the skin. According to the image presentation, after using the product for 2 weeks and 4 weeks, the collagen content in the subject's skin increased significantly, indicating that the lotion can increase the thickness of the dermis, improve skin elasticity, and can better improve the aging of the underlying skin; according to the VISIA image analysis results, after using the lotion sample for 4 weeks, the subjects' wrinkles improved by an average of 17.94%, and the skin roughness decreased by 8.44%, indicating that the lotion has a good improvement effect on wrinkles and roughness of aging skin; after using the lotion sample for 4 weeks, the subject's facial redness value decreased by 5.53%, the skin moisture content increased by 26.3%, and the transepidermal water loss TWEL value decreased by 8.25%, indicating that the lotion sample has a good repair effect on the skin barrier, can moisturize and repair the skin at the same time, and improve skin redness and other inflammatory conditions. This lotion can improve skin aging while repairing the sensitive state of the skin, and is suitable for the anti-aging field of sensitive skin. See the improvement effect diagram of some cases. Figures 1 to 3 .

[0071] Table 10. Change rate of human efficacy test index of emulsion particles in experimental example 2

[0072] In summary, the present invention provides a composition and its emulsion and application with sensitive skin repair and anti-aging effects. The active composition and its emulsion can significantly promote the growth of keratinocytes and fibroblasts, inhibit inflammatory factors, repair the skin barrier, and thus reduce the dryness, redness and burning of sensitive skin; at the same time, it can significantly promote the production of type I, type IV and type VII collagen, laminin-5 and integrin β1 in the skin, improve skin elasticity and firmness, reduce wrinkles, enhance skin fineness and smoothness, and can be used in anti-aging care products for sensitive skin.

[0073] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions claimed to be protected by the present invention.

Claims

1. A composition with sensitive skin repair and anti-aging functions, characterized in that: The composition comprises the following components: Component A: Pseudoalteromonas ferment extract, collagen and palmitoyl tripeptide-5; Component B: acetyl glucosamine, sodium hyaluronate and silanetriol; Component C: Caffeine, Acetyl Hexapeptide-8.

2. A composition with sensitive skin repair and anti-aging effects according to claim 1, wherein The composition comprises the following components, in parts by weight: Pseudoalteromonas fermentation product extract: 0.15-5 parts; Collagen: 0.003-0.05 parts; Palmitoyl tripeptide-5: 0.0001-0.1 parts; Acetyl glucosamine: 0.05-3 parts; Sodium hyaluronate: 0.1018-0.135 parts; Silanetriol: 0.0015-0.03 parts; Caffeine: 0.1-0.5 parts; Acetyl hexapeptide-8: 0.0002-0.1 parts.

3. A composition with sensitive skin repair and anti-aging effects according to claim 2, characterized in that: The composition includes the following components, calculated in parts by weight: 0.25 parts of Pseudoalteromonas fermentation product extract, 0.005 parts of collagen, 0.0005 parts of palmitoyl tripeptide-5, 1 part of acetyl glucosamine, 0.107 parts of sodium hyaluronate, 0.006 parts of silanetriol, 0.5 parts of caffeine, and 0.001 parts of acetyl hexapeptide-8.

4. An emulsion prepared by using the composition according to any one of claims 1 to 3, characterized in that: described The 40°C ΔBS value of the emulsion is 0.30%-5%; the -20°C ΔBS value of the emulsion is 2%-5%.

5. The emulsion according to claim 4, characterized in that The particle size of the emulsion is 750-790 nm.

6. The emulsion according to claim 4, characterized in that The preparation method of the emulsion comprises the following specific steps: (1) Preparation of oil phase: weigh the oil and emulsifier in the oil phase, heat in a water bath, stir and keep warm. until completely dissolved into the oil phase; (2) Preparation of thickening phase: weigh the thickener, mix it with the moisturizer, add water, heat and swell, and form To form a thickening phase; (3) Mix the oil phase and thickening phase homogeneously, stir and cool; (4) adding the composition, moisturizer and preservative, and stirring to obtain the emulsion.

7. The emulsion according to claim 6, characterized in that The oil includes at least one of isononyl isononanoate, shea butter and polydimethylsiloxane; the emulsifier includes at least one of SIMULSOL165, C12-20 acid PEG-8 ester and cetyl alcohol; the thickener includes at least one of ammonium acryloyldimethyltaurate / VP copolymer, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer and xanthan gum; the moisturizer includes at least one of tocopheryl acetate and sorbitol.

8. The emulsion according to claim 7, characterized in that In parts by weight, the amount of the oil is 0-8 parts of isononyl isononanoate, 0-6 parts of avocado butter, and 1 part of polydimethylsiloxane; the amount of the emulsifier is 0-0.8 parts of SIMULSOL 165, 0-2.5 parts of C12-20 acid PEG-8 ester, and 0-2 parts of cetyl alcohol; the amount of the thickener is 0-0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer, 0-0.15 parts of acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, and 0-0.1 parts of xanthan gum; the amount of the moisturizer is 0-2 parts of tocopheryl acetate and 0-2 parts of sorbitol.

9. The emulsion according to claim 4, characterized in that In parts by mass, the optimal formula of the emulsion is: 4 parts of isononyl isononanoate; 3 parts of shea butter; 1 part of polydimethylsiloxane; 1 part of tocopheryl acetate; 2 parts of cetyl alcohol; 0.8 parts of SIMULSOL 165; 2.5 parts of C12-20 acid PEG-8 esters; 0.3 parts of ammonium acryloyldimethyltaurate / VP copolymer; 0.05 parts of xanthan gum; 1 part of sorbitol; 0.15 parts of acrylates / C10-30 alkyl acrylate crosspolymer.

10. Use of the composition according to any one of claims 1 to 3 or the emulsion according to claim 4 in the preparation of cosmetics with sensitive skin repair and anti-aging functions.

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