Boa-oil-containing composition for prevention and adjuvant therapy of bedsore and preparation method of boa-oil-containing composition

Abstract

The invention provides a python oil-containing composition for prevention and adjuvant therapy of bedsore and a preparation method thereof. The python oil-containing composition comprises the following raw materials in parts by weight: 15-25 parts of python oil, 10-30 parts of pseudo-ginseng, 10-30 parts of dragon's blood, 10-20 parts of saussurea palustris, 10-20 parts of oldenlandia diffusa and 5-15 parts of a compound extract, the compound extract is a sea-buckthorn extract and a green tea extract. According to the boa-oil-containing composition for prevention and adjuvant therapy of bedsore, a pressure sore rat model is constructed, TNF-alpha in rat serum is reduced after treatment is conducted for 3 days, the local inflammatory response of a bedsore wound on the body surface of a rat can be effectively inhibited, the curative effect of wound healing is accelerated, the wound area in 3 days is 1.76-1.96 cm < 2 >, and the treatment effect is good. The boa oil-containing composition can promote wound healing, and has the effects of rapidly promoting tissue regeneration and the like.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and in particular to a python oil-containing composition for preventing and assisting in treating bedsores and a preparation method thereof. Background Art

[0002] Decubitus, also known as pressure sores, pressure ulcers, or pressure injuries, are caused by sustained pressure on localized tissue, leading to impaired blood circulation, ischemia, hypoxia, and nutrient deficiency, resulting in loss of normal skin function and tissue breakdown and necrosis. Long-term bed rest, restricted mobility, and prolonged pressure on the skin are the primary causes of decubitus ulcers. The formation of decubitus ulcers is a complex process, resulting from the combined effects of local and systemic factors, leading to skin tissue deformation and necrosis. Traditional treatments for decubitus ulcers involve cleaning the wound surface and applying sterile dressings, but these methods are slow to respond and can easily lead to systemic infection, further exacerbating the condition. Both traditional Chinese and Western medicine are conducting extensive research on the treatment of decubitus ulcers, and the development of effective medications that shorten treatment times remains a hot topic. Numerous traditional Chinese medicine preparations are commercially available for the treatment of decubitus ulcers, such as Qili Powder and Xiaoyan Shengji Cream. These can further shorten treatment times for early-stage decubitus ulcers, improving patient quality. Summary of the Invention

[0003] In view of this, the object of the present invention is to provide a python oil-containing composition for preventing and assisting in the treatment of bedsores and a preparation method thereof.

[0004] The technical solution of the present invention is achieved as follows:

[0005] A python oil-containing composition for preventing and assisting in the treatment of bedsores, comprising the following raw materials in parts by weight: 15-25 parts of python oil, 10-30 parts of Panax notoginseng, 10-30 parts of Dragon's Blood, 10-20 parts of Saussurea dasyphylla, 10-20 parts of Hedyotis diffusa, and 5-15 parts of a compound extract;

[0006] The compound extract is sea buckthorn extract and green tea extract.

[0007] Panax notoginseng (Burk.) FHChen, a plant of the Araliaceae family, is a dried root. It enters the Liver and Stomach meridians, and is sweet, slightly bitter, and warm in nature. Its functions include dispersing blood stasis, arresting bleeding, and relieving swelling and pain. It is used for hemoptysis, hematemesis, epistaxis, bloody stools, metrorrhagia, bleeding from trauma, chest and abdominal pain, and swelling and pain from falls.

[0008] Xuejie (Dragon's Blood) is made from the resin exuded from the fruit of Daemonorops draco Blume (palmaceae). It enters the Heart and Liver meridians and is sweet, salty, and neutral in nature. It can dispel blood stasis, relieve pain, and stop bleeding and promote tissue regeneration. It is used for fractures and bruises, internal injuries, and persistent bleeding from external injuries.

[0009] Saussurea arenaria, also known as Saussurea arenaria Maxim, is a plant of the Asteraceae family. The volatile oil from the whole plant is rich in β-selinene, dihydrodehydrocostuslactone, 4-methyl-2,6-di-tert-butylphenol, 6,10,14-trimethyl-2-pentadecanone, octadecane, and 1,1,2-tetradecane. Octadecane, nonadecane, 1,1,4,7-tetram-ethyl-1α,2,3,4,4α,5,6,7b-octahydro-1H-cycloprop[e]azulene, pentadecane, 4-methoxy-1-tert-butyloxybenzene, etc. It tastes pungent and bitter, and is cold in nature. Its main functions include treating wind-heat on the surface, detoxifying, and stopping bleeding. It is used to treat wind-heat colds, sores, carbuncles, and bleeding from trauma.

[0010] Hedyotis diffusa (Willd.) Roxb., a plant of the Rubiaceae family, is the whole herb. Hedyotis diffusa (Willd.) Roxb., a plant of the genus Hedyotis, has been isolated from the whole herb. Heteronesane, stigmasterol, ursolic acid, oleanolic acid, β-sitosterol, β-sitosterol-D-glucoside, and p-coumaric acid are among the components of this herb. Modern pharmacology suggests that Hedyotis diffusa has antibacterial and anti-inflammatory properties. It enters the stomach, large intestine, and small intestine meridians, and has a sweet, mild, and cooling nature. Its main functions include clearing away heat and toxic substances, promoting diuresis and reducing swelling, and promoting blood circulation and alleviating pain. It is used for intestinal carbuncle (appendicitis), sores, boils, damp-heat jaundice, and dysuria. It is also used externally to treat sores, boils, carbuncles, and snake bites.

[0011] Seabuckthorn is the dried mature fruit of Hippophae rhamnoides L., a plant of the genus Hippophae in the family Elaeagnaceae. It is sour, astringent, and warm in nature. Its main functions include relieving cough and expectoration, promoting digestion and relieving stagnation, promoting blood circulation and dispersing blood stasis. It is used for cough with excessive sputum, indigestion, abdominal pain caused by food stagnation, amenorrhea caused by blood stasis, and swelling caused by falls. Seabuckthorn extract is rich in vitamin E, carotene, carotenoids, β-sitosterol, and unsaturated fatty acids, which can inhibit the development of subcutaneous inflammation, enhance the anti-inflammatory effects of inflammatory centers, and significantly promote ulcer healing.

[0012] Panax notoginseng and Sanguisorba officinalis have the effects of promoting blood circulation, removing blood stasis, stopping bleeding and promoting tissue regeneration. Saussurea dasyphylla and Hedyotis diffusa have the effects of clearing away heat and detoxifying, and having antibacterial and anti-inflammatory effects. Combined with the effects of python oil in moisturizing the skin, reducing inflammation and swelling, as well as enhancing cell vitality and accelerating metabolism, it helps to accelerate pus discharge, promote tissue regeneration and relieve pain. Combined with the antioxidant and inflammatory relief effects of sea buckthorn extract and green tea extract, it can improve the body's immune function, enhance the body's disease resistance, and has a certain anti-allergic effect, further accelerating the healing rate of bedsore wounds and improving the quality of treatment.

[0013] A further solution comprises the following raw materials in parts by weight: 20-25 parts of python oil, 12-25 parts of Panax notoginseng, 12-25 parts of dragon's blood, 12-15 parts of Saussurea dasyphylla, 12-15 parts of Hedyotis diffusa and 5-10 parts of the compound extract;

[0014] The compound extract is sea buckthorn extract and green tea extract in a mass ratio of 1-2:1-2.

[0015] A further solution comprises the following raw materials in parts by weight: 20-25 parts of python oil, 15-20 parts of Panax notoginseng, 15-20 parts of Sanguisorba officinalis, 12-15 parts of Saussurea dasyphylla, 12-15 parts of Hedyotis diffusa and 5-8 parts of the compound extract.

[0016] A further solution comprises the following raw materials in parts by weight: 22 parts of python oil, 18 parts of Panax notoginseng, 18 parts of Sanguisorba officinalis, 13 parts of Saussurea dasyphylla, 13 parts of Hedyotis diffusa and 6 parts of the compound extract.

[0017] A further solution is that the preparation method of the seabuckthorn extract comprises: taking seabuckthorn fruit, adding ice water to pulp, performing ultrasonic extraction at 30-40° C., centrifuging, taking the supernatant, and freeze-drying to obtain the seabuckthorn extract;

[0018] The mass volume ratio of the seabuckthorn fruit and ice water is 1: (6-10) g / mL;

[0019] The temperature of the ice water is 4-6°C;

[0020] The power of the ultrasonic extraction is 600-800W, and the time of the ultrasonic extraction is 1-2h.

[0021] In one embodiment of the present invention, ice water is provided for seabuckthorn fruit pulping, and ultrasonic extraction at a relatively low temperature is used to facilitate the full extraction of active ingredients from the seabuckthorn fruit and reduce the loss of active ingredients.

[0022] In a further embodiment, the preparation method of the green tea extract comprises: grinding green tea, adding water for extraction, filtering, and freeze-drying the filtrate to obtain the green tea extract;

[0023] The mass volume ratio of the green tea and water is 1: (8-15) g / mL;

[0024] The extraction temperature is 85-95° C., and the extraction time is 6-8 hours.

[0025] Preferably, the present invention provides a method for preparing the above-mentioned python oil-containing composition for preventing and assisting in treating bedsores, comprising the following steps:

[0026] S1 decoction: according to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water and decoct, filter, and concentrate the filtrate to obtain a decoction;

[0027] S2 mixing: according to the formula, the decoction in step S1 is mixed with python oil and the compound extract to obtain the python oil-containing composition.

[0028] A further solution is that in step S1, the mass volume ratio of the medicinal material and water is 1: (10-20) g / mL.

[0029] Preferably, the present invention also provides the use of the above-mentioned python oil-containing composition for preventing and assisting in treating bedsores in inhibiting inflammatory factors.

[0030] In a further embodiment, the inflammatory factor is TNF-α.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention selects python oil, Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera, Hedyotis diffusa and a compound extract, wherein the compound extract is sea buckthorn extract and green tea extract, and designs a python oil-containing composition for preventing and assisting in treating bedsores. Panax notoginseng and Sanguisorba officinalis have the effects of promoting blood circulation, removing blood stasis, stopping bleeding and promoting tissue regeneration, while Saussurea dactylifera and Hedyotis diffusa have the effects of clearing away heat and detoxifying, and having antibacterial and anti-inflammatory effects. Combined with the effects of python oil on moisturizing the skin, reducing inflammation and swelling, enhancing cell activity, and accelerating metabolism, the composition helps to accelerate pus discharge, promote tissue regeneration and relieve pain. Combined with the effects of sea buckthorn extract and green tea extract on anti-oxidation and relieving inflammatory reactions, the composition can improve the body's immune function, enhance the body's disease resistance, and has a certain anti-allergic effect, further accelerating the healing rate of bedsore wounds and improving the quality of treatment.

[0033] By constructing a pressure ulcer rat model, the TNF-α level in rat serum decreased after 3 days of treatment, effectively inhibiting the local inflammatory response of the pressure ulcer wound on the rat body surface and accelerating the healing of the wound. The wound area at 3 days was 1.76-1.96 cm 2 , indicating that the composition containing python oil can promote wound healing and has the effects of rapid tissue regeneration. DETAILED DESCRIPTION

[0034] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0035] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0036] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0037] The python oil used in the examples and comparative examples of the present invention can be commercially available python oil.

[0038] Example 1

[0039] A python oil-containing composition for preventing and assisting in the treatment of bedsores, comprising (by weight) 22 parts of python oil, 18 parts of Panax notoginseng, 18 parts of Sanguisorba officinalis, 13 parts of Saussurea dactylifera, 13 parts of Hedyotis diffusa, and 6 parts of a compound extract, wherein the compound extract comprises a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0040] The preparation method of sea buckthorn extract is as follows: take sea buckthorn fruit, add ice water at 4-6°C according to the mass-to-volume ratio of material to liquid of 1g:8mL, and fully beat it, then place it in a water bath at 30-40°C, adjust the ultrasonic power to 600W, and perform ultrasonic extraction for 1h. After the ultrasonic extraction is completed, low-temperature centrifugation is performed, and the supernatant is taken and freeze-dried to obtain the sea buckthorn extract.

[0041] The preparation method of green tea extract is as follows: grind green tea, pass it through a 100-120 mesh sieve, add 85-95°C water according to a material-liquid mass volume ratio of 1g:12mL, adjust the temperature to 85-95°C and perform hot water extraction for 6 hours, filter after the hot water extraction is completed, and freeze-dry the filtrate to obtain the green tea extract.

[0042] The preparation method of the composition is:

[0043] (1) Decoction: According to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water at a mass-to-volume ratio of 1 g:15 mL, decoct, filter after decoction, and concentrate the filtrate to obtain a decoction;

[0044] (2) Mixing: According to the formula, the obtained decoction is mixed with python oil and the compound extract to obtain the python oil-containing composition.

[0045] Example 2

[0046] A python oil-containing composition for preventing and assisting in the treatment of bedsores, comprising (by weight) 25 parts of python oil, 30 parts of Panax notoginseng, 10 parts of Sanguisorba officinalis, 10 parts of Saussurea dactylifera, 20 parts of Hedyotis diffusa, and 15 parts of a compound extract, wherein the compound extract comprises a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0047] The preparation method of sea buckthorn extract is as follows: take sea buckthorn fruit, add ice water at 4-6°C according to the mass-to-volume ratio of material to liquid of 1g:8mL, and fully beat it, then place it in a water bath at 30-40°C, adjust the ultrasonic power to 600W, and perform ultrasonic extraction for 1h. After the ultrasonic extraction is completed, low-temperature centrifugation is performed, and the supernatant is taken and freeze-dried to obtain the sea buckthorn extract.

[0048] The preparation method of green tea extract is as follows: grind green tea, pass it through a 100-120 mesh sieve, add 85-95°C water according to a material-liquid mass volume ratio of 1g:12mL, adjust the temperature to 85-95°C and perform hot water extraction for 6 hours, filter after the hot water extraction is completed, and freeze-dry the filtrate to obtain the green tea extract.

[0049] The preparation method of the composition is:

[0050] (1) Decoction: According to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water at a mass-to-volume ratio of 1 g:15 mL, decoct, filter after decoction, and concentrate the filtrate to obtain a decoction;

[0051] (2) Mixing: According to the formula, the obtained decoction is mixed with python oil and the compound extract to obtain the python oil-containing composition.

[0052] Example 3

[0053] A python oil-containing composition for preventing and assisting in the treatment of bedsores, comprising (by weight) 15 parts of python oil, 12 parts of Panax notoginseng, 25 parts of Sanguisorba officinalis, 20 parts of Saussurea dactylifera, 20 parts of Hedyotis diffusa, and 5 parts of a compound extract, wherein the compound extract comprises a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0054] The preparation method of sea buckthorn extract is as follows: take sea buckthorn fruit, add ice water at 4-6°C according to the mass-to-volume ratio of material to liquid of 1g:8mL, and fully beat it, then place it in a water bath at 30-40°C, adjust the ultrasonic power to 600W, and perform ultrasonic extraction for 1h. After the ultrasonic extraction is completed, low-temperature centrifugation is performed, and the supernatant is taken and freeze-dried to obtain the sea buckthorn extract.

[0055] The preparation method of green tea extract is as follows: grind green tea, pass it through a 100-120 mesh sieve, add 85-95°C water according to a material-liquid mass volume ratio of 1g:12mL, adjust the temperature to 85-95°C and perform hot water extraction for 6 hours, filter after the hot water extraction is completed, and freeze-dry the filtrate to obtain the green tea extract.

[0056] The preparation method of the composition is:

[0057] (1) Decoction: According to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water at a mass-to-volume ratio of 1 g:15 mL, decoct, filter after decoction, and concentrate the filtrate to obtain a decoction;

[0058] (2) Mixing: According to the formula, the obtained decoction is mixed with python oil and the compound extract to obtain the python oil-containing composition.

[0059] Example 4

[0060] A python oil-containing composition for preventing and assisting in the treatment of bedsores, comprising (by weight) 22 parts of python oil, 20 parts of Panax notoginseng, 20 parts of Sanguisorba officinalis, 15 parts of Saussurea dactylifera, 12 parts of Hedyotis diffusa, and 15 parts of a compound extract, wherein the compound extract comprises a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0061] The preparation method of sea buckthorn extract is as follows: take sea buckthorn fruit, add ice water at 4-6°C according to the mass-to-volume ratio of material to liquid of 1g:8mL, and fully beat it, then place it in a water bath at 30-40°C, adjust the ultrasonic power to 600W, and perform ultrasonic extraction for 1h. After the ultrasonic extraction is completed, low-temperature centrifugation is performed, and the supernatant is taken and freeze-dried to obtain the sea buckthorn extract.

[0062] The preparation method of green tea extract is as follows: grind green tea, pass it through a 100-120 mesh sieve, add 85-95°C water according to a material-liquid mass volume ratio of 1g:12mL, adjust the temperature to 85-95°C and perform hot water extraction for 6 hours, filter after the hot water extraction is completed, and freeze-dry the filtrate to obtain the green tea extract.

[0063] The preparation method of the composition is:

[0064] (1) Decoction: According to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water at a mass-to-volume ratio of 1 g:15 mL, decoct, filter after decoction, and concentrate the filtrate to obtain a decoction;

[0065] (2) Mixing: According to the formula, the obtained decoction is mixed with python oil and the compound extract to obtain the python oil-containing composition.

[0066] Comparative Example 1

[0067] The difference between this comparative example and Example 1 is that the dosage of the formula is adjusted, and the formula (by weight) is: 5 parts of python oil, 35 parts of Panax notoginseng, 18 parts of Sanguisorba officinalis, 25 parts of Saussurea dasyphylla, 25 parts of Hedyotis diffusa and 6 parts of the compound extract, which is a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0068] Comparative Example 2

[0069] The difference between this comparative example and Example 1 is that python oil is replaced by horse oil, and the formula (by weight) is: 22 parts of horse oil, 18 parts of Panax notoginseng, 18 parts of Sanguisorba officinalis, 13 parts of Saussurea dasyphylla, 13 parts of Hedyotis diffusa and 6 parts of the compound extract, which is a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0070] Comparative Example 3

[0071] The difference between this comparative example and Example 1 is that Saussurea dactylifera is replaced by honeysuckle, and the formula (by weight) is: 22 parts of python oil, 18 parts of Panax notoginseng, 18 parts of Sanguisorba officinalis, 13 parts of honeysuckle, 13 parts of Hedyotis diffusa and 6 parts of the compound extract, which is a sea buckthorn extract and a green tea extract in a mass ratio of 2:1.

[0072] Test Example - Rat Model

[0073] Experimental animals: 45 SPF healthy male SD rats weighing 200 g to 220 g.

[0074] Drug: the pharmaceutical composition prepared in Examples 1 to 4 and Comparative Examples 1 to 3 of the present invention.

[0075] Modeling: Rats were anesthetized with 10% chloral hydrate (350 mg / kg) and depilated in the center of the back. After disinfection with iodine, a transverse incision approximately 3 cm deep to the fascia was made. The tissue was bluntly dissected, and a sterilized iron sheet (25 mm × 25 mm × 0.5 mm) was implanted in the deep fascia of the back. The incision was sutured and disinfected with iodine. On the second postoperative day, a magnet (25 mm × 20 mm × 1 mm) was placed in place of the iron sheet. A cycle of 2 hours of ischemia and 30 minutes of reperfusion was repeated three times daily for three consecutive days. The formation of a Stage III pressure ulcer was determined by darkening and hardening of the skin at the pressure site and the absence of bleeding after needle puncture. After modeling, the iron sheet was removed, and the wound was sutured and cleaned.

[0076] Treatment: After 7 days of adaptive feeding, the rats with successful modeling were randomly divided into a blank group, a model group and a drug group. The blank group and the model group were smeared with iodine tincture, and the drug group was given medication (0.5 g / kg) after smearing with iodine tincture, once a day for 3 consecutive days. During the treatment period, the rats had free access to food and water, and the environment was kept clean.

[0077] Testing: After the end of treatment, 2 mL of abdominal aortic blood was collected from each group of animals under anesthesia. The supernatant was centrifuged, aliquoted into EP tubes, and stored in a -80°C freezer until use. Serum TNF-α levels were measured according to the kit instructions.

[0078] Table 1 TNF-α levels in serum and wound area in rats of each group (n=5)

[0079]

[0080]

[0081] As shown in Table 1 above, the serum TNF-α content of the rats in the model group was the highest. After treatment with different compositions in the drug-administered groups, the serum TNF-α content of the drug-administered groups decreased to varying degrees compared with the model group. The serum TNF-α content of drug-administered groups 1 to 4 decreased the most, indicating that the python oil-containing composition obtained by selecting python oil, Panax notoginseng, Dragon's Blood, Saussurea dactylifera, Hedyotis diffusa, and compound extracts (sea buckthorn extract and green tea extract) of the present invention can inhibit the local inflammatory response of pressure ulcer wounds on the body surface of rats and accelerate wound healing. The wound area on day 3 was 1.76 to 1.96 cm 2 , indicating that the composition containing python oil can promote wound healing and has the effects of rapid tissue regeneration.

[0082] Panax notoginseng and Sanguisorba officinalis have the effects of promoting blood circulation, removing blood stasis, stopping bleeding and promoting tissue regeneration. Saussurea dasyphylla and Hedyotis diffusa have the effects of clearing away heat and detoxifying, and having antibacterial and anti-inflammatory effects. Combined with the effects of python oil in moisturizing the skin, reducing inflammation and swelling, as well as enhancing cell vitality and accelerating metabolism, it helps to accelerate pus discharge, promote tissue regeneration and relieve pain. Combined with the antioxidant and inflammatory relief effects of sea buckthorn extract and green tea extract, it can improve the body's immune function, enhance the body's disease resistance, and has a certain anti-allergic effect, further accelerating the healing rate of bedsore wounds and improving the quality of treatment.

[0083] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A composition containing python oil for preventing and assisting in the treatment of bedsores, characterized in that: The invention comprises the following raw materials in parts by weight: 15-25 parts of python oil, 10-30 parts of Panax notoginseng, 10-30 parts of dragon's blood, 10-20 parts of Saussurea dasyphylla, 10-20 parts of Hedyotis diffusa and 5-15 parts of compound extract; The compound extract is sea buckthorn extract and green tea extract.

2. The python oil-containing composition for preventing and assisting in treating bedsores according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 20-25 parts of python oil, 12-25 parts of Panax notoginseng, 12-25 parts of dragon's blood, 12-15 parts of Saussurea dasyphylla, 12-15 parts of Hedyotis diffusa and 5-10 parts of compound extract; The compound extract is sea buckthorn extract and green tea extract in a mass ratio of 1-2:1-2.

3. The python oil-containing composition for preventing and assisting in treating bedsores according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 20-25 parts of python oil, 15-20 parts of Panax notoginseng, 15-20 parts of dragon's blood, 12-15 parts of Saussurea dasyphylla, 12-15 parts of Hedyotis diffusa and 5-8 parts of compound extract.

4. The python oil-containing composition for preventing and assisting in treating bedsores according to claim 3, characterized in that: The invention comprises the following raw materials in parts by weight: 22 parts of python oil, 18 parts of Panax notoginseng, 18 parts of dragon's blood, 13 parts of Saussurea dasyphylla, 13 parts of Hedyotis diffusa and 6 parts of compound extract.

5. The python oil-containing composition for preventing and assisting in treating bedsores according to claim 1, characterized in that: The preparation method of the seabuckthorn extract comprises: taking seabuckthorn fruit, adding ice water to pulp, performing ultrasonic extraction at 30-40° C., centrifuging, taking the supernatant, and freeze-drying to obtain the seabuckthorn extract; The mass volume ratio of the seabuckthorn fruit and ice water is 1: (6-10) g / mL; The temperature of the ice water is 4-6°C; The power of the ultrasonic extraction is 600-800W, and the time of the ultrasonic extraction is 1-2h.

6. The python oil-containing composition for preventing and assisting in treating bedsores according to claim 1, characterized in that: The preparation method of the green tea extract comprises: grinding green tea, adding water for extraction, filtering, and freeze-drying the filtrate to obtain the green tea extract; The mass volume ratio of the green tea and water is 1: (8-15) g / mL; The extraction temperature is 85-95° C., and the extraction time is 6-8 hours.

7. The method for preparing a composition containing python oil for preventing and assisting in treating bedsores according to any one of claims 1 to 6, characterized in that: The steps include: S1 decoction: according to the formula, take Panax notoginseng, Sanguisorba officinalis, Saussurea dactylifera and Hedyotis diffusa, add water and decoct, filter, and concentrate the filtrate to obtain a decoction; S2 mixing: according to the formula, the decoction in step S1 is mixed with python oil and the compound extract to obtain the python oil-containing composition.

8. The method for preparing a composition containing python oil for preventing and assisting in treating bedsores according to claim 7, characterized in that: In step S1, the mass volume ratio of the medicinal material to water is 1: (10-20) g / mL.

9. Use of the python oil-containing composition for preventing and assisting in treating bedsores according to any one of claims 1 to 6 in inhibiting inflammatory factors.

10. The use according to claim 9, characterized in that The inflammatory factor is TNF-α.

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