Preparation method and application of medicinal and edible oral liquid with effects of dispelling effects of alcohol and protecting liver

Abstract

The invention belongs to the field of life health and health products, and discloses a preparation method and application of medicinal and edible oral liquid with effects of dispelling effects of alcohol and protecting liver. The raw material formula comprises a ginseng extract, hovenia dulcis thunb, radix puerariae, roselle, rhizoma polygonati, gastrodia elata, schisandra chinensis and a dihydroquercetin extract, the hovenia dulcis thunb, the radix puerariae, the roselle, the rhizoma polygonati, the gastrodia elata and the schisandra chinensis are taken in proportion, water reflux extraction and filtration are performed, extracting solutions are combined, the ginseng extract and the dihydroquercetin extract are added, and filling and sterilization are performed to obtain the traditional Chinese medicine composition. Functions of the raw materials are fully utilized, the oral liquid is capable of relieving drunkenness, enlivening spleen, protecting liver and benefiting gallbladder, and behavioral observation of a mouse acute alcoholism experiment proves that the oral liquid can delay drunkenness, accelerate sobering up and relieve swelling pain of brain and liver, drunkenness and the like caused by excessive drinking. Enzymatic index detection of a mouse acute alcoholism experiment proves that the oral liquid can quickly improve the alcohol decomposition capability of the liver after being taken, delay the absorption of alcohol in the body and achieve the effects of dispelling the effects of alcohol and relieving drunkenness.

Description

Technical Field

[0001] The present invention belongs to the field of life health and health care products, and relates to the technical field of liver disease treatment research and functional product development. It mainly provides a preparation method and application of a medicine-food oral liquid with alcohol-relieving and liver-protecting effects, specifically the application of a medicine-food formula in the preparation of a drug for protecting chemical liver damage and a health food for alcohol-relieving and liver-protecting effects. Background Art

[0002] Regular alcohol consumption can cause severe damage to multiple organs, including the heart, brain, liver, pancreas, and kidneys. Excessive drinking can weaken the heart muscle, cause brain damage, and lead to depression and memory loss. Excessive drinking can also lead to alcoholic cirrhosis and even alcoholic hepatitis, and cause pancreatic dysfunction and pancreatitis. Excessive alcohol consumption can also lead to high blood pressure, the second leading cause of kidney failure.

[0003] According to the latest data released by The Lancet, approximately 300 million people worldwide suffer from alcoholic liver damage, with 2 million developing cirrhosis each year and resulting in 1 million deaths. The latest data from the China Liver Disease Burden Report shows that the number of liver disease patients in my country has reached an alarming 400 million, with 60 million suffering from alcoholic liver disease. Over 60% of these patients are at risk of developing cirrhosis or even liver cancer.

[0004] What needs to be paid close attention to is that the quantity issue is not the only risk. According to the statistical results of scientific research big data, nearly 50% of individuals in Asia have alcohol intolerance (only 30% in other regions), and the activity of acetaldehyde dehydrogenase, which metabolizes alcohol in their bodies, is relatively low. Long-term excessive drinking can cause fatty degeneration of liver cells, and the risk of developing alcoholic fatty liver is 5 to 25 times higher than that of ordinary people. When the liver's metabolic function declines, the incidence of 61 diseases, including stroke and myocardial infarction, will increase to 68%. Therefore, the development of drugs or health foods that can sober up and protect the liver is of great significance for the treatment of liver disease and drug research and development.

[0005] While a wide variety of alcohol-detoxifying and liver-protecting products have been developed in recent years, most contain complex ingredients and are slow to take effect, requiring a certain amount of time to take effect. This inability to effectively detoxify alcohol in a timely manner results in limited effectiveness. The existing art also lacks any public reports on the alcohol-detoxifying and liver-protecting effects of the present invention's formulation. Summary of the Invention

[0006] Against this backdrop and to address the shortcomings of the prior art, the present invention, through extensive innovative experiments, provides a medicinal and edible formula, extract preparation method, and application thereof, with alcohol sobering and liver-protecting effects. This formula is highly safe and has excellent liver-protecting effects. It can effectively lower transaminases, triglycerides, and blood lipids, enhance antioxidant activity, and accelerate metabolism to prevent and improve alcoholic fatty liver disease. Experiments have confirmed that this formula can enhance liver metabolic capacity and improve liver function, effectively achieving the goals of liver protection and alcohol sobering.

[0007] The medicinal and edible formula comprises ginseng extract, Hovenia dulcis fruit, Pueraria root, Hibiscus sabdariffa, Polygonatum sibiricum, Gastrodia elata, Schisandra chinensis, and dihydroquercetin extract. Based on the relationship between monarch, minister, assistant, and envoy drugs in traditional Chinese medicine prescriptions, in this formula, ginseng extract is the monarch drug, Hovenia dulcis fruit and Pueraria root are minister drugs, Hibiscus sabdariffa, Polygonatum sibiricum, and Gastrodia elata are adjuvant drugs, and Schisandra chinensis and dihydroquercetin extract are envoy drugs.

[0008] Ginseng (Panax ginseng CA ley.), a perennial herb in the Araliaceae family, has the effects of nourishing the five internal organs, calming the mind, soothing the soul, calming palpitations, dispelling pathogenic qi, and improving eyesight and intelligence. Modern pharmacological research has revealed that ginsenosides, the main active ingredient in ginseng, are the material basis for its hepatoprotective effects. Hovenia dulcis (Hovenia aurantium) is a plant of the Rhamnaceae family, known for its ability to expand blood volume, promote urine excretion, and accelerate intestinal motility, thereby accelerating alcohol metabolism and excretion, reducing alcohol concentration in the body and achieving the effect of sobering up. Pueraria root (Kuang) is the dried root of the Fabaceae plant, known for its detoxifying and spleen-stimulating properties. Its sweet and mild nature and flavor are effective in detoxifying alcohol, invigorating the spleen and stomach, and quenching thirst. It is used to treat symptoms such as headache, dizziness, thirst, vomiting, and chest fullness caused by excessive drinking. Solanum sabdariffa (Sabdariffa) is an annual herb in the Malvaceae family, containing a variety of natural medicinal ingredients and antioxidants that enhance the liver's detoxification capacity, reduce alcohol damage, and have a significant effect in sobering up. Polygonatum sibiricum, a plant of the Liliaceae family, boasts numerous benefits, including nourishing yin and moistening the lungs, tonifying the spleen and replenishing qi, enhancing immunity, and delaying aging. It can mitigate the harmful effects of alcohol and has a certain hangover-relief effect. Gastrodia elata, a plant of the genus Gastrodia in the Orchidaceae family, has the effects of calming wind and relieving spasms, calming liver yang, and dispelling wind and unblocking the meridians. It can be used to treat symptoms such as internal liver wind, epilepsy, convulsions, dizziness, and headaches. Drinking a moderate amount of Gastrodia elata water after a hangover can alleviate the discomfort caused by intoxication to a certain extent and accelerate alcohol metabolism, thus aiding in hangover relief. The fruit of Schisandra chinensis contains schisandrin, vitamin C, resins, tannins, and a small amount of sugars. It has the effects of astringing the lungs and relieving coughs, nourishing and astringing essence, and stopping diarrhea and sweating. Schisandra chinensis extract can reduce elevated alanine aminotransferase levels in experimental animals induced by carbon tetrachloride and thioethanolamine. Schisandrin B has anti-liver damage effects. Furthermore, schisandrin has a central nervous system sedative effect. Dihydroquercetin is a new food resource derived from larch. It can inhibit lipogenesis and protect the liver by regulating the expression of related transcription factors. Based on the effects of the aforementioned Chinese medicinal and edible herbs in alcohol detoxification and liver protection, they were scientifically formulated according to their proportions to produce the oral liquid formulation of the present invention.

[0009] The extract preparation method comprises the following steps:

[0010] (1) Take ginseng root, soak it in 60-95% edible alcohol for 12-24 hours, then heat and reflux and extract it 1-3 times, each time for 1-3 hours, combine the extracts, concentrate under reduced pressure, and apply non-polar macroporous adsorption resin D101 to the extract, and elute it with water, 30% ethanol and 70% ethanol in sequence. Collect the fraction eluted with 70% ethanol, and recover the solvent to obtain a crude extract. Add 3-8 times the amount of water to the residue after the above ginseng extraction, extract it multiple times at 80-100℃, each time for 1-3 hours, combine the extracts, and concentrate under reduced pressure to obtain a ginseng crude enzyme solution. Mix the ginseng crude extract and the ginseng crude enzyme solution in a weight ratio of 1:1-10, and react at 30-40℃ for 40-50 hours. After the reaction, ginseng extract is obtained, and the total saponin content should be 70-95%.

[0011] (2) Take the larch root, crush it, and then extract it with 75% ethanol solution under reflux, filter it, collect the filtrate, and concentrate it to obtain an extract. The extract is dispersed and dissolved in water, applied to a macroporous adsorption resin column, and eluted with water and ethanol solution in sequence. The ethanol eluate is collected and concentrated to obtain a dihydroquercetin extract, and the dihydroquercetin content should be above 80%.

[0012] (3) Extract Hovenia dulcis, Pueraria root, Solanum sabdariffa, Polygonatum sibiricum, Gastrodia elata, and Schisandra chinensis with water under reflux, filter, and obtain a filtrate mother liquor. Add the ginseng extract and dihydroquercetin extract to each liter of the filtrate and mix well.

[0013] (4) Filling and sterilization.

[0014] The extract preparation method comprises weighing 5-20 parts of Hovenia dulcis fruit, 5-18 parts of Pueraria root, 2-8 parts of Hibiscus sabdariffa, 2-10 parts of Polygonatum sibiricum, 1-5 parts of Gastrodia elata, and 1-4 parts of Schisandra chinensis.

[0015] In the extract preparation method, the amount of ginseng extract added per liter of filtrate is equivalent to 5-30g of ginseng.

[0016] In the extract preparation method, 1-15 g of dihydroquercetin extract is added per liter of filtrate.

[0017] The medicine-food oral liquid can improve liver function, assist in protecting chemical liver damage, increase the SOD level of the liver after drinking, reduce the MDA level, improve liver function biochemical indicators, and protect the liver.

[0018] The medicine-food oral liquid can increase the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, enhance the alcohol metabolism ability of the liver, delay drunkenness, quickly sober up, and achieve the effect of sobering up.

[0019] The daily dosage of the medicine-food oral liquid for adults is 10-100 mL.

[0020] The medicine-food oral liquid can be concentrated and dried into powder and then taken with water.

[0021] The medicine-food oral liquid can also be made into ointment, granules, tablets, solid beverages and the like.

[0022] The present invention provides a preparation method and application of a medicinal and edible oral liquid with the effects of sobering up and protecting the liver. It has been found that the prescription has significant effects of sobering up and protecting the liver, and can be used to produce health care products such as protecting chemical liver damage and sobering up. The beneficial effects of the present invention are that the medicinal and edible prescription is highly safe, easy to take, and can be drunk directly. After taking this product, the human body's tolerance to alcohol can be enhanced, symptoms such as dizziness, fatigue, vomiting, rapid heartbeat, and increased blood pressure caused by drinking can be alleviated, and symptoms of slow reaction and nervousness that occur after drinking can be alleviated. Experiments have confirmed that the prescription can enhance the liver's alcohol metabolism capacity, improve liver function, and can well achieve the purposes of liver protection and sobering up, and has a good market prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 To study the effects of different formula combinations of medicine-food oral liquid on the intoxication time and sobering time of mice with acute alcohol poisoning; Figure 1 A shows the effects of different formula combinations of medicine-food oral solution on righting time and sleep time in mice with acute alcohol poisoning; Figure 1 B shows the effects of different formula combinations of medicine-food oral liquid on the awakening time and recovery time of mice with acute alcohol poisoning; Figure 1 C is the effect of different formula combinations of medicine-food oral liquid on the drunkenness rate of mice with acute alcohol poisoning; Figure 1 D is the effect of different formula combinations of medicine-food oral liquid on the blood ethanol concentration of mice with acute alcohol poisoning;

[0024] Figure 2 To study the effects of different formula combinations of medicine-food oral liquid on alcohol dehydrogenase, acetaldehyde dehydrogenase and liver function biochemical indexes in mice with acute alcohol poisoning. Figure 2 A is the effect of different formula combinations of medicine-food oral liquid on the activity of alcohol dehydrogenase in mice with acute alcohol poisoning; Figure 2 B is the effect of different formula combinations of medicine-food oral liquid on acetaldehyde dehydrogenase activity in mice with acute alcohol poisoning; Figure 2 C is the effect of different formula combinations of medicine-food oral liquid on ALT activity in mice with acute alcohol poisoning; Figure 2 D is the effect of different formula combinations of medicine-food oral liquid on AST activity in mice with acute alcohol poisoning; Figure 2 E is the effect of different formula combinations of medicine-food oral liquid on SOD enzyme activity in mice with acute alcohol poisoning; Figure 2 F is the effect of different formula combinations of medicine-food oral liquid on MDA content in mice with acute alcohol poisoning;

[0025] Figure 3 The effect of medicine-food oral solution on the intoxication time and sobering time of acute alcohol poisoning mice; Figure 3 A shows the effects of different doses of medicine-food oral solution on righting time and sleep time in mice with acute alcohol poisoning; Figure 3 B shows the effects of different doses of medicine-food oral solution on the awakening time and recovery time of mice with acute alcohol poisoning; Figure 3 C is the effect of different doses of medicine-food oral solution on the drunkenness rate of mice with acute alcohol poisoning; Figure 3 D is the effect of different doses of medicine-food oral solution on the blood ethanol concentration in mice with acute alcohol poisoning;

[0026] Figure 4 This study aimed to investigate the effects of the food-drug oral solution on alcohol dehydrogenase, acetaldehyde dehydrogenase and liver function biochemical indices in mice with acute alcohol poisoning. Figure 4 A shows the effects of different doses of medicine-food oral solution on the activity of alcohol dehydrogenase in mice with acute alcohol poisoning; Figure 4 B is the effect of different doses of medicine-food oral solution on acetaldehyde dehydrogenase activity in mice with acute alcohol poisoning; Figure 4 C is the effect of different doses of medicine-food oral solution on ALT activity in mice with acute alcohol poisoning; Figure 4 D is the effect of different doses of medicine-food oral solution on AST activity in mice with acute alcohol poisoning; Figure 4 E is the effect of different doses of medicine-food oral solution on SOD enzyme activity in mice with acute alcohol poisoning; Figure 4 F shows the effects of different doses of medicine-food oral solution on MDA content in mice with acute alcohol poisoning. DETAILED DESCRIPTION

[0027] The present invention is further described in detail below in conjunction with experiments and specific embodiments. However, this should not be understood as limiting the scope of the present invention to the following embodiments, and all technologies implemented based on the present invention fall within the scope of the present invention.

[0028] Example 1 Comparison of formulas of alcohol-relieving and liver-protecting medicinal and edible oral liquids.

[0029] (1) Take ginseng root, soak it in 60% edible alcohol for 12 hours, then heat and reflux and extract it twice, each time for 1 hour, combine the extracts, concentrate under reduced pressure, and apply the extract to non-polar macroporous adsorption resin D101, eluting it with water, 30% ethanol and 70% ethanol in sequence. Collect the fraction eluted with 70% ethanol, and recover the solvent to obtain a crude extract. Add 5 times the amount of water to the residue after the above ginseng extraction, extract it twice at 100°C, each time for 2 hours, combine the extracts, and concentrate under reduced pressure to obtain a ginseng crude enzyme solution. Mix the ginseng crude extract and the ginseng crude enzyme solution in a weight ratio of 1:1 and react at 37°C for 48 hours. After the reaction, the ginseng extract is obtained, and the content of ginsenosides Re, Rb1, Rg3, Rh2, Rg4, Rg6 and other saponins is detected to be 73%.

[0030] (2) The roots of larch were ground and then reflux-extracted with 75% ethanol solution twice for 2 hours each time. The extract was filtered, the filtrate was collected, and concentrated to obtain an extract. The extract was dispersed and dissolved in water, applied to a macroporous adsorption resin column, and eluted with water and ethanol solution in sequence. The ethanol eluate was collected and concentrated to obtain a dihydroquercetin extract. The dihydroquercetin content was detected to be 91%.

[0031] (3) Weigh 5 parts of Hovenia dulcis, 5 parts of Pueraria root, 2 parts of Hibiscus sabdariffa, 2 parts of Polygonatum sibiricum, 1 part of Gastrodia elata, and 1 part of Schisandra chinensis. Add water at a 10-fold solid-liquid ratio and reflux extract twice for 1 hour each time. Filter to obtain the filtrate mother liquor, which is used to prepare Formula 1.

[0032] (4) Add ginseng extract equivalent to 20 g of ginseng per liter of filtrate mother liquor and mix well to make formula 2.

[0033] (5) Add 5 g of dihydroquercetin extract to each liter of filtrate mother liquor and mix well to make Formula 3.

[0034] (6) Add ginseng extract equivalent to 20 g of ginseng and 5 g of dihydroquercetin extract to each liter of filtrate mother liquor, mix well, and prepare the formula.

[0035] Example 2

[0036] Effects of different formulas of alcohol-detoxifying and liver-protecting medicinal and edible oral liquid on the detoxification and liver protection of alcohol-poisoned mice.

[0037] 1. Establishment and Treatment of Alcohol Intoxication Mouse Model

[0038] Forty SPF-grade ICR male mice were adaptively reared for five days and then divided into five groups of eight mice each, including a control group, a formula 1 group (10 mL / kg body weight), a formula 2 group (10 mL / kg body weight), a formula 3 group (10 mL / kg body weight), and a formula group (10 mL / kg body weight). Each group of mice was fasted for 12 hours but not water. At the beginning of the experiment, mice were gavaged with normal saline and oral solutions of different formulas based on their body weight. Thirty minutes later, mice were gavaged with 50% alcohol (12 mL / kg body weight, approximately the amount a human drinks in one sitting). The state of intoxication and duration of each group of mice were observed.

[0039] 2. Behavioral responses to intoxication and alcohol metabolism in mice

[0040] After administering alcohol orally, mice were turned over on their backs every 1 minute. If the mice could maintain the back-down position for more than 30 seconds, their righting reflex was lost and they were considered intoxicated. Righting time: the time from alcohol administration to the loss of the righting reflex. Sleep time: the time from alcohol administration to the onset of sleep. Awakening time: the time from alcohol administration to the return of the righting reflex. Recovery time: the time from alcohol administration to the return of free movement. Intoxication rate: the number of mice in each group with loss of righting reflex / the number of mice in each group * 100%.

[0041] After the mice were gavaged with alcohol, blood was collected from the eye sockets of the mice in each group at 1 hour and 3 hours respectively. The serum was collected by centrifugation and the ethanol concentration in the blood was measured using an ethanol content determination kit.

[0042] Righting time and falling asleep time are comprehensive reflections of the incubation period of mice intoxication, while awakening time and recovery time are comprehensive reflections of mice’s hangover recovery. In the hangover recovery experiment of acute alcohol poisoning, after oral administration of large amounts of alcohol, mice in all groups showed symptoms of slow movement, lack of energy, and drowsiness. Figure 1As shown, the righting time and sleep onset time were 12.1 minutes and 36.8 minutes for the control group, 16.5 minutes and 42.3 minutes for the formulation 1 group, 23.5 minutes and 49.7 minutes for the formulation 2 group, 25.3 minutes and 50.5 minutes for the formulation 3 group, and 29.9 minutes and 58.2 minutes for the formulation group. This indicates that the different formulations of the medicinal and edible oral liquid prolonged the incubation period of intoxication in mice, with the formulation group being the most significant. The awakening time and recovery time were 223 minutes and 295 minutes for the control group, 181 minutes and 243 minutes for the formulation 1 group, 165 minutes and 224 minutes for the formulation 2 group, 157 minutes and 217 minutes for the formulation 3 group, and 110 minutes and 179 minutes for the formulation group, shortening the sobering and recovery time of the mice. The drunkenness rate was 100% in the control group, 87.5% in the formula 1 group, 75% in the formula 2 group, 87.5% in the formula 3 group, and 66.7% in the formula group. It can be seen that each treatment group reduced the drunkenness rate of alcohol-poisoned mice, and the formula had a significant alcohol-relieving effect.

[0043] The ethanol level in the blood reflects the amount of alcohol in the mouse's body and is directly related to the intoxication reaction. Figure 1 As shown in the figure, the serum ethanol concentrations 1 hour and 3 hours after oral administration of alcohol were 95 mg / 100 mL and 42 mg / 100 mL in the control group, 85 mg / 100 mL and 30 mg / 100 mL in the formula 1 group, 74 mg / 100 mL and 25 mg / 100 mL in the formula 2 group, 76 mg / 100 mL and 23 mg / 100 mL in the formula 3 group, and 68 mg / 100 mL and 12 mg / 100 mL in the formula group. It can be seen that each treatment group can reduce the blood ethanol level after drinking alcohol, among which the formula has the strongest alcohol-relieving effect, which reflects the synergistic effect of ginseng extract and dihydroquercetin extract in the formula.

[0044] 3. Determination of liver tissue biochemical indicators

[0045] After the mice were adapted to feeding, they were divided into 5 groups, including control group, formula group 1, formula group 2, formula group 3, and formula group. Each group of mice was fasted for 12 hours but not water. At the beginning of the experiment, they were gavaged with normal saline, different doses of the formula, and positive drugs according to their body weight. After 30 minutes, 50% alcohol (12mL / kg body weight) was gavaged, and the mouse livers were taken after another 30 minutes. An appropriate amount of liver tissue was weighed, ground with 9 times normal saline, and then the ground liquid was centrifuged at 4°C and 3000rpm for 10 minutes. The supernatant was taken and tested for alcohol dehydrogenase, acetaldehyde dehydrogenase, ALT, AST, SOD, MDA, etc. All indicators were tested according to the operating instructions of the kit.

[0046] The enzymes involved in the metabolism of ethanol in the body include alcohol dehydrogenase and acetaldehyde dehydrogenase. Alcohol dehydrogenase can oxidize ethanol into acetaldehyde, which is further decomposed into acetic acid under the catalysis of acetaldehyde dehydrogenase and then excreted from the body through metabolism. Figure 2 As shown, the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase were 0.08 U / mL and 0.5 U / mL in the control group, 0.11 U / mL and 1.1 U / mL in the formulation 1 group, 0.19 U / mL and 1.6 U / mL in the formulation 2 group, 0.15 U / mL and 1.5 U / mL in the formulation 3 group, and 0.27 U / mL and 2.1 U / mL in the formulation 2 group. This indicates that the different formulations of the medicinal and edible oral liquid can all increase the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver of mice, and the formulation 2 in particular has a significant ability to accelerate alcohol metabolism.

[0047] The medicinal and edible formula improved the liver function and biochemical indicators of mice. ALT and AST levels can reflect the damage of liver function, while SOD and MDA reflect the oxidative protection and oxidative damage. Figure 2 As shown in the data, after mice drank a lot of alcohol and suffered acute alcohol poisoning, the liver ALT and AST levels were 108.7U / L and 74.6U / L in the control group, 83.8U / L and 52.1U / L in the formula 1 group, 72.1U / L and 42.7U / L in the formula 2 group, 83.2U / L and 48.9U / L in the formula 3 group, and 60.3U / L and 38.7U / L in the formula group. Different formulas of medicine and food can reduce the liver ALT and AST levels to varying degrees. At the same time, the SOD and MDA levels were 8.4 Units and 4.6 nmol / mgprot in the control group, 9.9 Units and 3.2 nmol / mgprot in the formula 1 group, 12.3 Units and 2.9 nmol / mgprot in the formula 2 group, 11.9 Units and 3.1 nmol / mgprot in the formula 3 group, and 13.8 Units and 2.5 nmol / mgprot in the combined formula group. It can be seen that different formulas can reduce MDA and increase SOD enzyme activity. The overall effect shows that the combined formula has the best liver-protecting effect, which comprehensively reflects the significant alcohol-relieving and liver-protecting effects.

[0048] Example 3

[0049] A preparation method of a hangover-relieving and liver-protecting medicinal and edible oral liquid, specifically comprising:

[0050] (1) Take ginseng root, soak it in 60% edible alcohol for 12 hours, then heat and reflux and extract it twice, each time for 1 hour. Combine the extracts and concentrate under reduced pressure. Apply the extract to non-polar macroporous adsorption resin D101 and elute it with water, 30% ethanol and 70% ethanol in sequence. Collect the fraction eluted with 70% ethanol and recover the solvent to obtain a crude extract. Add 5 times the amount of water to the residue after the above ginseng extraction, extract it twice at 100°C, each time for 2 hours. Combine the extracts and concentrate under reduced pressure to obtain a ginseng crude enzyme solution. Mix the ginseng crude extract and the ginseng crude enzyme solution in a weight ratio of 1:1 and react at 37°C for 48 hours. After the reaction, ginseng extract is obtained. The content of ginsenosides Re, Rb1, Rg3, Rh2, Rg4, Rg6 and other saponins was detected to be 72%.

[0051] (2) The roots of larch were ground and then reflux-extracted with 75% ethanol solution twice for 2 hours each time. The extract was filtered, the filtrate was collected, and concentrated to obtain an extract. The extract was dispersed and dissolved in water, applied to a macroporous adsorption resin column, and eluted with water and ethanol solution in sequence. The ethanol eluate was collected and concentrated to obtain a dihydroquercetin extract. The dihydroquercetin content was detected to be 92%.

[0052] (3) Weigh 5 parts of Hovenia dulcis, 5 parts of Pueraria root, 2 parts of Solanum sabdariffa, 2 parts of Polygonatum sibiricum, 1 part of Gastrodia elata, and 1 part of Schisandra chinensis. Add water at a 10-fold solid-liquid ratio and reflux extract twice for 1 hour each time. Filter to obtain the filtrate mother liquor.

[0053] (4) Add 10 g of ginseng extract and 1 g of dihydroquercetin extract per liter of filtrate mother liquor and mix well.

[0054] (5) Filling and sterilization to obtain the oral solution.

[0055] Example 4

[0056] A preparation method of a hangover-relieving and liver-protecting medicinal and edible oral liquid, specifically comprising:

[0057] (1) Take ginseng root, soak it in 75% edible alcohol for 24 hours, then heat and reflux and extract it twice, each time for 2 hours. Combine the extracts and concentrate under reduced pressure. Apply the extract to non-polar macroporous adsorption resin D101 and elute it with water, 30% ethanol and 70% ethanol in sequence. Collect the fraction eluted with 70% ethanol and recover the solvent to obtain a crude extract. Add 5 times the amount of water to the residue after the above ginseng extraction, extract it twice at 100°C, each time for 2 hours. Combine the extracts and concentrate under reduced pressure to obtain a ginseng crude enzyme solution. Mix the ginseng crude extract and the ginseng crude enzyme solution in a weight ratio of 1:5 and react at 37°C for 48 hours. After the reaction, the ginseng extract is obtained. The content of ginsenosides Re, Rb1, Rg3, Rh2, Rg4, Rg6 and other saponins is detected to be 82%.

[0058] (2) Take the larch root, crush it, and then reflux extract it with 75% ethanol solution twice, each time for 2 hours, filter it, collect the filtrate, and concentrate it to obtain the extract. The extract is dispersed and dissolved in water, applied to a macroporous adsorption resin column, and eluted with water and ethanol solution in sequence. The ethanol eluate is collected and concentrated to obtain the dihydroquercetin extract.

[0059] (3) Weigh 10 parts of Hovenia dulcis, 12 parts of Pueraria root, 4 parts of Solanum sabdariffa, 6 parts of Polygonatum sibiricum, 3 parts of Gastrodia elata, and 2 parts of Schisandra chinensis. Add water at a 10-fold solid-liquid ratio and reflux extract twice for 1 hour each time. Filter to obtain the filtrate mother liquor.

[0060] (4) Add 20 g of ginseng extract and 5 g of dihydroquercetin extract per liter of filtrate mother liquor and mix well.

[0061] (5) Filling and sterilization to obtain the oral solution.

[0062] Example 5

[0063] A preparation method of a hangover-relieving and liver-protecting medicinal and edible oral liquid, specifically comprising:

[0064] (1) Take ginseng root, soak it in 95% edible alcohol for 24 hours, then heat and reflux and extract it twice, each time for 2 hours. Combine the extracts and concentrate under reduced pressure. Apply the extract to non-polar macroporous adsorption resin D101 and elute it with water, 30% ethanol and 70% ethanol in sequence. Collect the fraction eluted with 70% ethanol and recover the solvent to obtain a crude extract. Add 5 times the amount of water to the residue after the above ginseng extraction, extract it twice at 100°C, each time for 2 hours. Combine the extracts and concentrate under reduced pressure to obtain a ginseng crude enzyme solution. Mix the ginseng crude extract and the ginseng crude enzyme solution in a weight ratio of 1:10 and react at 37°C for 48 hours. After the reaction, ginseng extract is obtained. The content of ginsenosides Re, Rb1, Rg3, Rh2, Rg4, Rg6 and other saponins is detected to be 95%.

[0065] (2) Take the root of larch, crush it, and then reflux extract it with 75% ethanol solution twice, each time for 2 hours. Filter it, collect the filtrate, and concentrate it to obtain an extract. The extract is dispersed and dissolved in water, applied to a macroporous adsorption resin column, and eluted with water and ethanol solution in sequence. The ethanol eluate is collected and concentrated to obtain a dihydroquercetin extract. The dihydroquercetin content is detected to be 95%.

[0066] (3) Weigh 20 parts of Hovenia dulcis, 18 parts of Pueraria root, 8 parts of Solanum sabdariffa, 10 parts of Polygonatum sibiricum, 5 parts of Gastrodia elata, and 4 parts of Schisandra chinensis. Add water at a 10-fold solid-liquid ratio and reflux extract twice for 1 hour each time. Filter to obtain the filtrate mother liquor.

[0067] (4) Add 30 g of ginseng extract and 15 g of dihydroquercetin extract per liter of filtrate mother liquor and mix well.

[0068] (5) Filling and sterilization to obtain the oral solution.

[0069] Example 6

[0070] An oral liquid for detoxifying alcohol and protecting the liver, which has medicinal and edible properties, has detoxifying and liver-protecting effects on alcohol-poisoned mice.

[0071] 1. Establishment and Treatment of Alcohol Intoxication Mouse Model

[0072] Forty SPF-grade ICR male mice were acclimated for five days and then divided into five groups of eight mice each, including a control group, an oyster extract group (10g / kg body weight) as a positive control, a low-dose group (10mL / kg body weight), a medium-dose group (10mL / kg body weight), and a high-dose group (10mL / kg body weight). Each group of mice was fasted for 12 hours but not water. At the beginning of the experiment, mice were gavaged with normal saline, oyster extract, and different doses of the formula according to their body weight. Thirty minutes later, mice were gavaged with 50% alcohol (12mL / kg body weight, approximately the amount of alcohol a person drinks in one sitting). The state of intoxication and duration of each group of mice were observed.

[0073] Preparation of oyster extract: Take 1 kg of fresh oyster meat, mash it, add appropriate amount of water, heat and extract twice, filter, combine the extracts, refrigerate for 24 hours, fine filter, and adjust the concentration to contain 1 g / mL oyster meat.

[0074] 2. Behavioral responses to intoxication and alcohol metabolism in mice

[0075] After administering alcohol orally, mice were turned over on their backs every 1 minute. If the mice could maintain the back-down position for more than 30 seconds, their righting reflex was lost and they were considered intoxicated. Righting time: the time from alcohol administration to the loss of the righting reflex. Sleep time: the time from alcohol administration to the onset of sleep. Awakening time: the time from alcohol administration to the return of the righting reflex. Recovery time: the time from alcohol administration to the return of free movement. Intoxication rate: the number of mice in each group with loss of righting reflex / the number of mice in each group * 100%.

[0076] After the mice were gavaged with alcohol, blood was collected from the eye sockets of the mice in each group at 1 hour and 3 hours respectively. The serum was collected by centrifugation and the ethanol concentration in the blood was measured using an ethanol content determination kit.

[0077] Righting time and falling asleep time are comprehensive reflections of the incubation period of mice intoxication, while awakening time and recovery time are comprehensive reflections of mice’s hangover recovery. In the hangover recovery experiment of acute alcohol poisoning, after oral administration of large amounts of alcohol, mice in all groups showed symptoms of slow movement, lack of energy, and drowsiness. Figure 3As shown, the righting time and sleep onset time were 15.5 minutes and 38.6 minutes for the control group, 20 minutes and 39 minutes for the oyster extract group, 18.6 minutes and 47 minutes for the low-dose group, 28 minutes and 55 minutes for the medium-dose group, and 32.5 minutes and 52.7 minutes for the high-dose group. This indicates that the different dose groups of the medicinal and edible oral liquid significantly prolonged the incubation period of intoxication in mice. The awakening time and recovery time were 218.3 minutes and 273.8 minutes for the control group, 162 minutes and 211.7 minutes for the oyster extract group, 126.4 minutes and 230.8 minutes for the low-dose group, 99.5 minutes and 167.7 minutes for the medium-dose group, and 139.6 minutes and 156.3 minutes for the high-dose group, shortening the sobering and recovery time of the mice. The drunkenness rate was 100% in the control group, 75% in the oyster extract group, 66.7% in the low-dose group, 75% in the medium-dose group, and 75% in the high-dose group. It can be seen that each treatment group reduced the drunkenness rate of alcohol-poisoned mice, and the prescription had a significant alcohol-relieving effect.

[0078] The ethanol level in the blood reflects the amount of alcohol in the mouse's body and is directly related to the intoxication reaction. Figure 3 As shown in the figure, the serum ethanol concentrations 1 hour and 3 hours after oral administration of alcohol were 92.3 mg / 100 mL and 37.3 mg / 100 mL in the control group, 78.6 mg / 100 mL and 26.5 mg / 100 mL in the oyster extract group, 73.1 mg / 100 mL and 19.9 mg / 100 mL in the low-dose group, 68.5 mg / 100 mL and 15.6 mg / 100 mL in the medium-dose group, and 66.9 mg / 100 mL and 9.7 mg / 100 mL in the high-dose group. It can be seen that each treatment group can significantly reduce the blood ethanol level after drinking, which comprehensively reflects the alcohol-relieving effect of the prescription.

[0079] 3. Determination of liver tissue biochemical indicators

[0080] After the mice were adaptively raised, they were divided into 5 groups, including a control group, an oyster extract group, a low-dose group, a medium-dose group, and a high-dose group. Each group of mice was fasted for 12 hours but not water. At the beginning of the experiment, they were gavaged with normal saline, different doses of the formula, and a positive drug according to their body weight. After 30 minutes, 50% alcohol (12 mL / kg body weight) was gavaged, and the mouse livers were taken after another 30 minutes. An appropriate amount of liver tissue was weighed, ground with 9 times normal saline, and then the ground liquid was centrifuged at 4°C and 3000 rpm for 10 minutes. The supernatant was taken to measure alcohol dehydrogenase, acetaldehyde dehydrogenase, ALT, AST, SOD, MDA, etc. All indicators were tested according to the operating instructions of the kit.

[0081] The enzymes involved in the metabolism of ethanol in the body include alcohol dehydrogenase and acetaldehyde dehydrogenase. Alcohol dehydrogenase can oxidize ethanol into acetaldehyde, which is further decomposed into acetic acid under the catalysis of acetaldehyde dehydrogenase and then excreted from the body through metabolism. Figure 4 As shown, the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase were 0.059 U / mL and 0.496 U / mL in the control group, 0.125 U / mL and 0.672 U / mL in the oyster extract group, 0.129 U / mL and 1.721 U / mL in the low-dose group, 0.141 U / mL and 1.911 U / mL in the medium-dose group, and 0.234 U / mL and 2.716 U / mL in the high-dose group. This indicates that the different dose groups of the medicinal and edible oral liquid significantly increased the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver of mice, and have a significant ability to accelerate alcohol metabolism.

[0082] The medicinal and edible formula improved the liver function and biochemical indicators of mice. ALT and AST levels can reflect the damage of liver function, while SOD and MDA reflect the oxidative protection and oxidative damage. Figure 4 As shown, after mice drank a lot of alcohol and suffered acute alcohol poisoning, the ALT and AST levels in the liver were 120.8U / L and 60.9U / L in the control group, 88.4U / L and 48.4U / L in the oyster extract group, 82.1U / L and 44.3U / L in the low-dose group, 71.7U / L and 45.1U / L in the medium-dose group, and 64.4U / L and 40.5U / L in the high-dose group. The medicine-food formula can reduce the ALT and AST levels in the liver. At the same time, the SOD and MDA levels were 10.84 Units and 5.17 nmol / mgprot in the control group, 13.69 Units and 3.64 nmol / mgprot in the oyster extract group, 13.95 Units and 2.89 nmol / mgprot in the low-dose group, 14.61 Units and 2.54 nmol / mgprot in the medium-dose group, and 15.78 Units and 2.25 nmol / mgprot in the high-dose group. It can be seen that the oral solution of the formula can reduce MDA and increase SOD enzyme activity, indicating that the formula has a liver-protective effect, and comprehensively reflects the significant alcohol-relieving and liver-protecting effects.

[0083] The above-described embodiments are only preferred embodiments of the present invention, and are not intended to be all feasible embodiments of the present invention. Any obvious modifications made by a person skilled in the art without departing from the principles and spirit of the present invention should be considered to be included within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing a medicinal and edible oral liquid with alcohol-relieving and liver-protecting effects, characterized in that: The following steps are involved: (1) Ginseng root is extracted with edible alcohol under hot reflux, concentrated under reduced pressure, and recovered after adsorption with a non-polar macroporous adsorption resin to obtain a crude ginseng extract. Water is added to the residue for further extraction, and concentrated under reduced pressure to obtain a crude ginseng enzyme solution. The crude ginseng extract and the crude ginseng enzyme solution are mixed in a weight ratio of 1:1-10, and reacted at 30-40°C for 40-50h to obtain a ginseng extract. (2) Take larch roots, crush them, and then extract them with 75% ethanol solution under reflux, filter, collect the filtrate, and concentrate to obtain an extract. Dissolve the extract in water, apply it to a macroporous adsorption resin column, and elute it with water and ethanol solution in sequence. Collect the ethanol eluate and concentrate it to obtain a dihydroquercetin extract; (3) extracting roselle, Hovenia dulcis, Pueraria root, Polygonatum sibiricum, Gastrodia elata, and Schisandra chinensis by reflux extraction with water, filtering, and obtaining a filtrate mother liquor. Adding ginseng extract and dihydroquercetin extract to each liter of filtrate and mixing uniformly; (4) Filling and sterilization.

2. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: In step (3), the raw materials are weighed in the following proportions: 5-20 parts of Hovenia dulcis, 5-18 parts of Pueraria root, 2-8 parts of Hibiscus sabdariffa, 2-10 parts of Polygonatum sibiricum, 1-5 parts of Gastrodia elata, and 1-4 parts of Schisandra chinensis.

3. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: The amount of ginseng extract added in step (3) per liter of filtrate is equivalent to 5-30 g of ginseng.

4. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: In step (3), 1-15 g of dihydroquercetin extract is added per liter of filtrate.

5. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: The preparation method of the crude ginseng extract in step (1) is specifically as follows: soaking in 60-95% edible alcohol for 12-24 hours, then heating and refluxing for extraction 1-3 times, each time for 1-3 hours, combining the extracts, concentrating under reduced pressure, applying the extracts to a non-polar macroporous adsorption resin D101, eluting with water, 30% ethanol and 70% ethanol in sequence, collecting the fraction eluted with 70% ethanol, and recovering the solvent to obtain the crude ginseng extract.

6. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: The preparation method of step (1) crude ginseng enzyme solution is as follows: adding 3-8 times the amount of water to the residue after ginseng extraction, extracting multiple times at 80-100° C. for 1-3 hours each time, combining the extracts, and concentrating under reduced pressure to obtain crude ginseng enzyme solution.

7. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: The total saponin content in the ginseng extract of step (1) is 70-95%.

8. The method for preparing the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 1, characterized in that: The dihydroquercetin content in the dihydroquercetin extract of step (1) is greater than 80%.

9. An application of a medicinal and edible oral liquid with alcohol-relieving and liver-protecting effects, characterized in that: The daily dosage of the medicine-food oral liquid for adults is 10-100 mL.

10. The use of the medicine-food oral liquid with alcohol-relief and liver-protection effects according to claim 9, characterized in that: The medicine-food oral liquid can be concentrated and dried into powder and then taken with water; it can also be made into ointment, granules, tablets, and solid beverages.

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