Decoction for promoting blood circulation, removing blood stasis and eliminating diseases and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and provides a decoction for promoting blood circulation to remove blood stasis and eliminating diseases and a preparation method thereof, and the decoction comprises the following components in parts by weight: 15-25 parts of pollen typhae, 15-25 parts of trogopterus dung, 15-25 parts of angelica sinensis, 12-18 parts of parched white peony root, 15-25 parts of pseudo-ginseng, 12-18 parts of curcuma zedoary, 15-25 parts of salvia miltiorrhiza, 10-15 parts of madder, 4-8 parts of liquorice, 15-25 parts of rhizoma cyperi, 12-18 parts of selfheal, 15-20 parts of ligusticum wallichii and 8-12 parts of leech. Aiming at the pathogenesis characteristics of meridian stasis and qi stagnation of patients with endometriosis, secondary dysmenorrhea, irregular menstruation, pelvic masses and ovarian cyst, the decoction for promoting blood circulation to remove blood stasis can effectively promote blood circulation to remove blood stasis, soften hardness to dissipate stagnation, promote qi circulation to relieve pain and improve the symptoms of endometriosis, secondary dysmenorrhea and the like, and the curative effect is exact.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines and relates to a blood-activating, blood-stasis-removing and symptom-eliminating soup and a preparation method thereof. Background Art

[0002] Endometriosis refers to the growth of functional endometrial tissue (glands and / or stroma) outside the uterine lining and myometrium. It can form nodules and masses, causing pain and infertility. 70%-80% of endometriosis patients experience varying degrees of pelvic pain, which varies in proportion to the severity of the disease. Symptoms include dysmenorrhea (typically secondary dysmenorrhea, which progressively worsens) and non-menstrual abdominal pain. Adenomyosis refers to the invasion of endometrial glands and stroma into the myometrium by various pathogenic factors, with increased menstrual flow, prolonged menstruation, and secondary, progressively worsening dysmenorrhea as the primary clinical manifestations.

[0003] Dysmenorrhea refers to pain that accompanies menstruation. The pain can occur before, during, or after menstruation, and is primarily concentrated in the lower abdomen. It is often cramping and is usually accompanied by other symptoms, including low back and leg pain, headache, dizziness, fatigue, nausea, vomiting, abdominal distension, and diarrhea. Secondary dysmenorrhea has a clear cause and is caused by organic pelvic diseases that cause abdominal pain during menstruation, such as endometriosis, adenomyosis, pelvic infection, endometrial polyps, submucosal fibroids, intrauterine adhesions, cervical stenosis, uterine malformation, pelvic congestion syndrome, and transverse vaginal septum. Traditional Chinese Medicine believes that dysmenorrhea is related to poor circulation of Qi and blood. Its cause is mostly due to obstruction of the Chong and Ren meridians or cold stagnation in the meridians. Pain occurs when there is obstruction, and the key to the pathogenesis is stasis.

[0004] While Western medicine is effective in relieving pain, it struggles to prevent recurrences. Hormone therapy also requires prolonged use and is often associated with adverse reactions. For women experiencing dysmenorrhea, preventing recurrences is as important as relieving pain. Traditional Chinese medicine (TCM) treats secondary dysmenorrhea caused by endometriosis and adenomyosis through a holistic approach, not only improving symptoms but also regulating the yin and yang balance of the body's internal organs, thereby addressing the underlying cause. Women with dysmenorrhea have diverse clinical syndromes, and TCM offers tailored treatment options. Some medications can also treat primary conditions such as endometriosis and adenomyosis that cause secondary dysmenorrhea. Compared to modern medical treatments, TCM treatments for dysmenorrhea are subject to fewer limitations, demonstrate good clinical efficacy in clinical studies, and are readily accepted. Furthermore, TCM can prevent recurrences of dysmenorrhea with fewer toxic side effects and adverse reactions than Western medicine, making it a viable alternative or complementary treatment for dysmenorrhea.

[0005] Shaofu Zhuyu Decoction, a classic prescription, originates from the book "Yi Lin Gaicuo," which explores the functions and physiological mechanisms of the human internal organs and details the causes and treatments of blood stasis symptoms. Shaofu Zhuyu Decoction is composed of 1.5g fennel root, 1.5g dried ginger, 3g Corydalis yanhusuo, 3g myrrh, 9g angelica root, 3g Chuanxiong root, 3g cinnamon bark, 6g red peony root, 9g pollen cattail, and 6g wulingzhi root. The angelica root, red peony root, and Chuanxiong root in this recipe promote blood circulation and dissipate blood stasis, replenishing blood and promoting blood circulation, regulating blood circulation and menstruation, dispelling stasis without harming the body and promoting new blood. Wulingzhi, pollen cattail, Corydalis yanhusuo, and myrrh promote blood circulation and dissipate stasis, while regulating qi and alleviating pain. Dried ginger, cinnamon bark, and fennel warm the meridians and dispel cold.

[0006] The prescription of Shaofu Zhuyu Decoction combined with Danzhi Xiaoyao Powder (Gu Xumeng, Liu Tongtong, Wang Xiaobin, et al. Exploration of the clinical application of Shaofu Zhuyu Decoction based on "Cong's Cold Region Gynecology" [J]. Jiangxi Traditional Chinese Medicine, 2025(4)) is 15g of fennel, 10g of roasted ginger, 15g of Corydalis yanhusuo, 15g of aconite root, 15g of frankincense, 15g of myrrh, 15g of angelica, 10g of pollen, 15g of cinnamon bark, 15g of red peony root, 10g of dried ginger, 5g of asarum, 1g of blood clotting agent, 10g of peony bark, 10g of gardenia, 20g of white peony root, 20g of bupleurum, 15g of poria, 15g of atractylodes macrocephala, 10g of licorice, and 15g of polygala. The prescription is complicated. Summary of the Invention

[0007] In light of this, the present invention provides a Huoxue Huayu Xiaozheng Decoction and its preparation method. The Huoxue Huayu Xiaozheng Decoction is derived from Shaofu Zhuyu Decoction. This decoction is specifically designed to effectively promote blood circulation, dispel stasis, soften and disperse nodules, promote qi circulation and relieve pain, and improve the symptoms of endometriosis and secondary dysmenorrhea, with a proven efficacy. This decoction addresses the pathogenesis of endometriosis, secondary dysmenorrhea, menstrual disorders, pelvic masses, and ovarian cysts, characterized by blood stasis blocking the meridians and qi stagnation.

[0008] The present invention also provides a preparation method of the blood-activating, blood-stasis-removing and syndrome-eliminating decoction.

[0009] The present invention also provides the use of the above-mentioned blood-activating, blood-stasis-removing and symptom-eliminating decoction in preparing a medicine for treating endometriosis, secondary dysmenorrhea, irregular menstruation, pelvic mass or ovarian cyst.

[0010] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are as follows:

[0011] The first aspect of the present invention provides a blood-activating, blood-stasis-removing and symptom-eliminating soup, which is prepared from the following raw materials, by weight: 15-25 parts of pollen, 15-25 parts of teff, 15-25 parts of angelica, 12-18 parts of stir-fried white peony root, 15-25 parts of Panax notoginseng, 12-18 parts of zedoaria, 15-25 parts of salvia miltiorrhiza, 10-15 parts of madder, 4-8 parts of liquorice, 15-25 parts of cyperus rotundus, 12-18 parts of selfheal, 15-20 parts of ligusticum, and 8-12 parts of leech.

[0012] As a preferred technical solution of the present invention, it is prepared from the following raw materials, which are as follows by weight: 20 parts of pollen, 20 parts of cinnamon bark, 20 parts of angelica, 15 parts of stir-fried white peony root, 20 parts of Panax notoginseng, 15 parts of Curcuma, 20 parts of Salvia miltiorrhiza, 12 parts of Rubia cordifolia, 6 parts of liquorice, 20 parts of Cyperus rotundus, 15 parts of Prunella vulgaris, 18 parts of Ligusticum chuanxiong and 10 parts of Hirudo.

[0013] As a preferred technical solution of the present invention, it is prepared from the following raw materials, which are as follows by weight: 15 parts of pollen, 25 parts of cinnamon bark, 15 parts of angelica, 18 parts of stir-fried white peony root, 25 parts of Panax notoginseng, 18 parts of Curcuma, 15 parts of Salvia miltiorrhiza, 15 parts of Rubia cordifolia, 8 parts of liquorice, 25 parts of Cyperus rotundus, 12 parts of Prunella vulgaris, 20 parts of Chuanxiong and 8 parts of Hirudo.

[0014] As a preferred technical solution of the present invention, it is prepared from the following raw materials, which are as follows by weight: 25 parts of pollen, 15 parts of cinnamon bark, 25 parts of angelica, 12 parts of stir-fried white peony root, 15 parts of Panax notoginseng, 12 parts of Curcuma, 25 parts of Salvia miltiorrhiza, 10 parts of Rubia cordifolia, 4 parts of liquorice, 15 parts of Cyperus rotundus, 18 parts of Prunella vulgaris, 15 parts of Ligusticum chuanxiong and 12 parts of Hirudo.

[0015] The second aspect of the present invention provides a preparation method of the above-mentioned blood-activating, blood-stasis-removing and syndrome-eliminating soup, comprising the following steps: weighing the raw materials according to the formula, mixing, adding 6-8 times the weight of the mixture in water to the mixture, soaking for 0.5-1h, decocting the obtained soaking liquid to 95-100°C, then cooling to 90-95°C and decocting for 0.5-1h, filtering to obtain a first decoction and medicinal residues; adding 4-6 times the weight of the medicinal residues in water to the medicinal residues, then boiling to 95-100°C, then cooling to 90-95°C and decocting for 0.5-1h, filtering to obtain a second decoction; and mixing the first decoction and the second decoction.

[0016] As a preferred technical solution of the present invention, the preparation method of the blood-activating, blood-stasis-removing and symptom-eliminating soup comprises the following steps: weighing raw materials according to the formula, mixing, adding water 6 times the weight of the mixture to the mixture, soaking for 1 hour, decocting the obtained soaking liquid to 95°C, then cooling to 95°C and decocting for 1 hour, filtering to obtain a first decoction and medicinal residues; adding water 6 times the weight of the medicinal residues to the medicinal residues, then boiling to 95°C, then cooling to 90°C and decocting for 0.5 hour, filtering to obtain a second decoction; and mixing the first decoction and the second decoction.

[0017] The third aspect of the present invention provides a traditional Chinese medicine preparation of Huoxue Huayu Xiaozheng Decoction, characterized in that the traditional Chinese medicine preparation is prepared using the above-mentioned Huoxue Huayu Xiaozheng Decoction as a raw material.

[0018] Furthermore, the Chinese medicine preparation also includes pharmaceutically acceptable excipients. The dosage form of the Chinese medicine preparation includes granules, capsules, tablets, powders, pills or oral liquids.

[0019] The fourth aspect of the present invention provides the use of the Huoxue Huayu Xiaozheng Decoction in preparing a medicament for preventing or treating endometriosis, secondary dysmenorrhea, irregular menstruation, pelvic mass or ovarian cyst caused by blood stasis in the meridians and qi stagnation.

[0020] The fifth aspect of the present invention provides use of the Huoxue Huayu Xiaozheng Decoction in preparing a medicament for preventing or treating endometriosis or secondary dysmenorrhea.

[0021] Formula analysis:

[0022] 1. Main medicine: Pollen Typhae, Ophiopogon japonicus

[0023] Puhuang: sweet in taste, neutral in nature, enters the liver and pericardium meridians, stops bleeding, resolves blood stasis, and relieves stranguria; used for hematemesis, epistaxis, hemoptysis, metrorrhagia, bleeding from trauma, amenorrhea, stabbing pain in the chest and abdomen, swelling and pain from falls, and painful stranguria caused by blood.

[0024] Wulingzhi (Oxyphylla) is bitter and sweet in flavor, warm in nature, and enters the liver and spleen meridians. Raw Wulingzhi promotes blood circulation and relieves pain, treating heart and abdominal pain, amenorrhea, and postpartum blood stasis. It can also be used externally to treat snake, scorpion, and centipede bites. Stir-fried, it can be used to stop bleeding and treat metrorrhagia, excessive menstruation, and persistent red discharge.

[0025] 2. Assistant herbs: Angelica sinensis, stir-fried white peony root, Panax notoginseng, Curcuma zedoaria

[0026] Angelica: spicy and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, metrorrhagia, abdominal pain due to deficiency and cold, paralysis, numbness of the skin, dry intestines and constipation, heaviness after dysentery, carbuncles, ulcers, and injuries from falls.

[0027] Fried white peony root: bitter and sour in taste, slightly cold in nature, enters the liver and spleen meridians, nourishes blood and regulates menstruation, astringes yin and stops sweating, softens the liver and relieves pain, and suppresses liver yang; used for blood deficiency and sallow complexion, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, cramps in the limbs, headache and dizziness.

[0028] Panax notoginseng: sweet, slightly bitter, warm in nature, enters the liver and stomach meridians, disperses blood stasis and stops bleeding, reduces swelling and relieves pain, and also has anti-thrombotic and anti-inflammatory effects and functions.

[0029] Curcuma: It tastes pungent and bitter, is warm in nature, and can promote qi and resolve blood, eliminate accumulation and relieve pain; it is used to treat heart pain caused by blood and qi, food stagnation, abdominal distension and pain, blood stasis and amenorrhea, dysmenorrhea, tumors and lumps, and injuries from falls.

[0030] 3. Adjuvants: Salvia miltiorrhiza, Rubia cordifolia, Licorice root

[0031] Salvia miltiorrhiza: bitter in taste, slightly cold in nature, enters the heart and liver meridians, removes blood stasis and relieves pain, promotes blood circulation and dredges menstruation, clears the heart and relieves restlessness; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, stabbing pain in the chest and abdomen, pain due to heat rheumatic arthritis, swollen and painful sores, restlessness and insomnia; hepatosplenomegaly, and angina pectoris.

[0032] Rubia cordifolia: bitter in taste, cold in nature, enters the liver meridian, cools blood, removes blood stasis, stops bleeding, and regulates menstruation. Used for hematemesis, epistaxis, metrorrhagia, traumatic bleeding, blood stasis, amenorrhea, joint pain, and swelling and pain from falls.

[0033] Licorice: sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians, tonifies the spleen and replenishes qi, clears away heat and detoxifies, eliminates phlegm and relieves cough, relieves urgency and stops pain, and harmonizes various medicines; it is used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, cramps and pain in the abdomen and limbs, carbuncles, swellings, and sores, and to relieve the toxicity and potency of drugs.

[0034] 4. Envoy drugs: Cyperus rotundus, Prunella vulgaris, Chuanxiong, Hirudo

[0035] Cyperus rotundus: It tastes pungent, slightly bitter and sweet, and is neutral in nature. It enters the lung, liver, spleen and stomach meridians, relieves liver depression, regulates qi and relieves fullness, regulates menstruation and relieves pain; it is used for liver depression and qi stagnation, chest and flank pain, hernia pain, breast pain, spleen and stomach qi stagnation, abdominal distension and pain, bloating and pain, irregular menstruation, amenorrhea and dysmenorrhea.

[0036] Prunella Vulgaris: Pungent, bitter, and cold in flavor, it enters the Liver and Gallbladder Meridians, specifically purging liver fire. It also improves eyesight, dissipates stagnation, and reduces swelling. According to Shennong's Herbal Classic, it "treats cold and heat, arthritis, carbuncle, rat bite, head sores, tumors, and relieves goiter and qi accumulation, as well as swelling and dampness in the feet."

[0037] Chuanxiong: Pungent and warm in flavor, it enters the liver and gallbladder meridians, promoting qi and relieving depression, dispelling wind and dampness, and promoting blood circulation and relieving pain. It is used to treat headaches and dizziness caused by wind-cold, flank pain and abdominal pain, cold arthritis and muscle spasms, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncles, and ulcers. It is also used for menstrual disorders, amenorrhea, dysmenorrhea, abdominal pain caused by masses, stabbing pain in the chest and flanks, swelling and pain caused by falls, headaches, and rheumatic pain.

[0038] Leech: salty and bitter in taste, neutral in nature, enters the liver meridian, breaks blood, removes blood stasis, and promotes menstruation; used for abdominal masses, blood stasis, amenorrhea, and injuries from falls.

[0039] The secret of compatibility:

[0040] In Huoxue Huayu Xiaozheng Decoction, Puhuang stops bleeding and dissolves blood stasis, while Wulingzhi promotes blood circulation and relieves pain. These two herbs dissolve blood stasis, activate blood circulation, and promote qi circulation and relieve pain, making them the main herbs. Angelica sinensis replenishes blood, activates blood circulation, regulates menstruation, and relieves pain. Stir-fried white peony root nourishes blood and regulates menstruation. Panax notoginseng dissipates blood stasis, stops bleeding, reduces swelling, and relieves pain. Curcuma zedoaria promotes qi circulation, dissolves blood stasis, and relieves accumulation. These four herbs enhance the main herbs' ability to dissolve blood stasis, activate blood circulation, and relieve pain, making them the assistant herbs. Salvia miltiorrhiza dissolves blood stasis, relieves pain, and promotes blood circulation and menstruation. Rubia cordifolia dissolves blood stasis, stops bleeding, and promotes menstruation. Licorice root tonifies the spleen and replenishes qi, clears heat, and detoxifies, making them the adjuvant herbs. Cyperus rotundus regulates qi, relieves depression, relieves pain, and regulates menstruation. Prunella vulgaris dissipates swelling, Chuanxiong promotes qi circulation, relieves depression, promotes blood circulation, and relieves pain. Hirudo dispels blood stasis and promotes menstruation, making them the guiding herbs. These herbs prevent the bitter and cold effects of the other herbs from stagnating qi and blood, while also relieving pain and alleviating symptoms. Overall, the prescription is both tonifying and purging, treating both the symptoms and the root causes, and has the effects of promoting blood circulation and removing blood stasis, softening and dispersing nodules, promoting qi and relieving pain.

[0041] The beneficial effects of the blood-activating, blood-stasis-removing and syndrome-eliminating decoction of the present invention are:

[0042] The blood-activating, blood-stasis-eliminating and symptom-eliminating decoction of the present invention significantly improves the pain symptoms of a model of dysmenorrhea secondary to endometriosis, reduces the degree of uterine swelling, improves and reduces uterine inflammation, and has a positive therapeutic effect on dysmenorrhea secondary to endometriosis.

[0043] Changing the components or compatibility ratios of the present invention's formula weakens the therapeutic effect of Huoxue Huayu Xiaozheng Decoction on dysmenorrhea secondary to endometriosis. The components of the present invention act synergistically. Huoxue Huayu Xiaozheng Decoction is superior to Shaofu Zhuyu Decoction in treating dysmenorrhea secondary to endometriosis.

[0044] The blood-activating, blood-stasis-removing and symptom-eliminating decoction of the present invention has low toxic and side effects and adverse reaction incidence, and can be used as a substitute or supplementary treatment for clinical dysmenorrhea. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 Comparison of the number of abdominal contractions among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05).

[0046] Figure 2 Comparison of uterine swelling among rats in each group. Different lowercase letters above the bar graph indicate significant differences among different groups (P<0.05). DETAILED DESCRIPTION

[0047] To validate the efficacy of the Huoxue Huayu Xiaozheng Decoction of the present invention, the inventors conducted pharmacodynamic studies. It should be noted that the drugs selected for the pharmacodynamic studies were those obtained from a representative formulation and preparation method of the present invention. Due to space limitations, the tests and results related to other formulations and preparation methods encompassed by the present invention are not exhaustively listed here.

[0048] Example 1 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0049] 20 parts of Pollen Typhae, 20 parts of Ophiopogon japonicus, 20 parts of Chinese Angelica sinensis, 15 parts of stir-fried white peony root, 20 parts of Panax notoginseng, 15 parts of Curcuma zedoariae, 20 parts of Salvia miltiorrhiza, 12 parts of Rubia cordifolia, 6 parts of Licorice root, 20 parts of Cyperus rotundus, 15 parts of Prunella vulgaris, 18 parts of Ligusticum chuanxiong and 10 parts of Hirudo.

[0050] The raw materials are weighed according to the formula, mixed, and water 6 times the weight of the mixture is added to the mixture, soaked for 1 hour, decocted the obtained soaking liquid to 95°C, then cooled to 95°C and decocted for 1 hour, and filtered to obtain a first decoction liquid and a medicinal residue; water 6 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 95°C, then cooled to 90°C and decocted for 0.5 hour, and filtered to obtain a second decoction liquid; and the first decoction liquid and the second decoction liquid are mixed.

[0051] Example 2 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0052] 15 parts of Pollen Typhae, 25 parts of Ophiopogon japonicus, 15 parts of Chinese Angelica sinensis, 18 parts of stir-fried white peony root, 25 parts of Panax notoginseng, 18 parts of Curcuma zedoariae, 15 parts of Salvia miltiorrhiza, 15 parts of Rubia cordifolia, 8 parts of Licorice root, 25 parts of Cyperus rotundus, 12 parts of Prunella vulgaris, 20 parts of Ligusticum chuanxiong and 8 parts of Hirudo.

[0053] The raw materials were weighed and mixed according to the formula, and water 8 times the weight of the mixture was added to the mixture, and the mixture was soaked for 0.5 minutes. The obtained soaking liquid was decocted to 100° C., and then cooled to 90° C. and decocted for 1 hour, and filtered to obtain a first decoction liquid and a medicinal residue; water 4 times the weight of the medicinal residue was added to the medicinal residue, and then boiled to 100° C., and then cooled to 95° C. and decocted for 1 hour, and filtered to obtain a second decoction liquid; the first decoction liquid and the second decoction liquid were mixed.

[0054] Example 3 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0055] 25 parts of Pollen Typhae, 15 parts of Ophiopogon japonicus, 25 parts of Chinese Angelica sinensis, 12 parts of stir-fried white peony root, 15 parts of Panax notoginseng, 12 parts of Curcuma zedoariae, 25 parts of Salvia miltiorrhiza, 10 parts of Rubia cordifolia, 4 parts of Licorice root, 15 parts of Cyperus rotundus, 18 parts of Prunella vulgaris, 15 parts of Ligusticum chuanxiong and 12 parts of Hirudo.

[0056] The raw materials were weighed and mixed according to the formula, and water 6 times the weight of the mixture was added to the mixture, and the mixture was soaked for 0.5 h. The obtained soaking liquid was decocted to 100° C., and then cooled to 95° C. and decocted for 0.5 h, and filtered to obtain a first decoction liquid and a medicinal residue; water 4 times the weight of the medicinal residue was added to the medicinal residue, and then boiled to 100° C., and then cooled to 95° C. and decocted for 0.5 h, and filtered to obtain a second decoction liquid; and the first decoction liquid and the second decoction liquid were mixed.

[0057] Example 4 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0058] 15 parts of Pollen Typhae, 20 parts of Ophiopogon japonicus, 25 parts of Chinese Angelicae Sinensis, 18 parts of stir-fried White Peony Root, 25 parts of Panax Notoginseng, 15 parts of Curcuma Rhizoma, 20 parts of Salvia miltiorrhiza, 15 parts of Rubia cordifolia, 6 parts of Licorice, 15 parts of Cyperus rotundus, 18 parts of Prunella Vulgaris, 18 parts of Ligusticum chuanxiong and 10 parts of Hirudo

[0059] The raw materials were weighed and mixed according to the formula, and water 8 times the weight of the mixture was added to the mixture, and the mixture was soaked for 0.5 h. The obtained soaking liquid was decocted to 95° C., and then cooled to 90° C. and decocted for 1 h, and filtered to obtain a first decoction liquid and a medicinal residue; water 4 times the weight of the medicinal residue was added to the medicinal residue, and then boiled to 95° C., and then cooled to 90° C. and decocted for 0.5 h, and filtered to obtain a second decoction liquid; the first decoction liquid and the second decoction liquid were mixed.

[0060] Example 5 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0061] 20 parts of Pollen Typhae, 15 parts of Ophiopogon japonicus, 15 parts of Chinese Angelica sinensis, 16 parts of stir-fried white peony root, 20 parts of Panax notoginseng, 18 parts of Curcuma zedoariae, 25 parts of Salvia miltiorrhizae, 10 parts of Rubia cordifolia, 4 parts of Licorice root, 25 parts of Cyperus rotundus, 16 parts of Prunella vulgaris, 15 parts of Ligusticum chuanxiong and 12 parts of Hirudo.

[0062] The raw materials were weighed and mixed according to the formula, and water 8 times the weight of the mixture was added to the mixture, and the mixture was soaked for 0.5 h. The obtained soaking liquid was decocted to 95° C., and then cooled to 90° C. and decocted for 1 h, and filtered to obtain a first decoction liquid and a medicinal residue; water 4 times the weight of the medicinal residue was added to the medicinal residue, and then boiled to 95° C., and then cooled to 90° C. and decocted for 1 h, and filtered to obtain a second decoction liquid; and the first decoction liquid and the second decoction liquid were mixed.

[0063] Example 6 A blood-activating, blood-stasis-removing and symptom-eliminating soup

[0064] 25 parts of Pollen Typhae, 25 parts of Ophiopogonis, 20 parts of Chinese Angelicae Sinensis, 12 parts of stir-fried White Peony Root, 15 parts of Panax Notoginseng, 12 parts of Curcuma Rhizoma, 15 parts of Salvia miltiorrhiza, 12 parts of Rubia cordifolia, 8 parts of Licorice, 20 parts of Cyperus rotundus, 12 parts of Prunella Vulgaris, 20 parts of Ligusticum chuanxiong and 8 parts of Hirudo.

[0065] The raw materials are weighed according to the formula, mixed, and water 6 times the weight of the mixture is added to the mixture, soaked for 1 hour, decocted the obtained soaking liquid to 95°C, then cooled to 95°C and decocted for 1 hour, and filtered to obtain a first decoction liquid and a medicinal residue; water 6 times the weight of the medicinal residue is added to the medicinal residue, then boiled to 95°C, then cooled to 90°C and decocted for 0.5 hour, and filtered to obtain a second decoction liquid; and the first decoction liquid and the second decoction liquid are mixed.

[0066] Comparative Example 1

[0067] 20 parts of Chinese Angelicae Sinensis, 15 parts of stir-fried white peony root, 20 parts of Panax notoginseng, 15 parts of Curcuma Rhizoma, 20 parts of Salvia miltiorrhiza, 12 parts of Rubia cordifolia, 6 parts of Licorice root, 20 parts of Cyperus rotundus, 15 parts of Prunella Vulgaris, 18 parts of Ligusticum chuanxiong and 10 parts of Hirudo.

[0068] Compared with Example 1, the difference is that it does not contain Typhae Pollen and Atractylodes Rhizoma, and the other components and dosages remain unchanged.

[0069] The preparation method is as in Example 1.

[0070] Comparative Example 2

[0071] 20 parts of Pollen Typhae, 20 parts of Ophiopogon japonicus, 20 parts of Radix Notoginseng, 15 parts of Rhizoma Curcumae, 20 parts of Salvia miltiorrhizae, 12 parts of Rubia cordifolia, 6 parts of Radix Glycyrrhizae, 20 parts of Rhizoma Cyperi, 15 parts of Spica Prunellae, 18 parts of Rhizoma Chuanxiong and 10 parts of Hirudo.

[0072] Compared with Example 1, the difference is that angelica and stir-fried white peony root are not contained, and other components and dosages remain unchanged.

[0073] The preparation method is as in Example 1.

[0074] Comparative Example 3

[0075] 20 parts of Pollen Typhae, 20 parts of Ophiopogon japonicus, 20 parts of Chinese Angelica sinensis, 15 parts of stir-fried white peony root, 20 parts of Panax notoginseng, 15 parts of Curcuma zedoaria, 6 parts of Licorice root, 20 parts of Cyperus rotundus, 15 parts of Prunella vulgaris, 18 parts of Ligusticum chuanxiong and 10 parts of Hirudo.

[0076] Compared with Example 1, the difference is that Salvia miltiorrhiza and Rubia cordifolia are not contained, and other components and dosages remain unchanged.

[0077] The preparation method is as in Example 1.

[0078] Comparative Example 4

[0079] Shaofu Zhuyu Decoction: fennel 1.5g, dried ginger 1.5g, Corydalis yanhusuo 3g, myrrh 3g, angelica 9g, Chuanxiong 3g, cinnamon 3g, red peony root 6g, pollen 9g, and aconite 6g.

[0080] The preparation method is as in Example 1.

[0081] Study on the effect of Huoxue Huayu Xiaozheng decoction on rats with endometritis-induced dysmenorrhea model

[0082] 1. Experimental Methods

[0083] Forty-eight female SD rats weighing 180-220 g were randomly selected as blank control group and 42 as modeling group after one week of adaptive feeding.

[0084] Rats in the modeling group were anesthetized with 5 mL / kg of 4% chloral hydrate. Abdominal hair was trimmed, and the skin was disinfected with 75% alcohol. A 2 cm midline incision was made in the lower abdomen to open the abdominal cavity. A transverse incision was made in the midsection of the left uterus. A sterile plastic tube (0.2 cm diameter, 0.5 cm long) disinfected with alcohol was placed in the uterus and sutured to the uterine incision to prevent it from falling off. 0.5 mL of 800,000 U penicillin sodium solution was dripped onto the wound. Oral administration began the next day after surgery.

[0085] After modeling, the rats were randomly divided into model control group, experimental group 1, experimental group 2, control group 1, control group 2, control group 3, and control group 4, with 6 rats in each group.

[0086] The experimental groups and gavage schedule are shown in Table 1.

[0087] Table 1 Oral gavage regimen for rats in each group

[0088] Group dose Oral dose Blank control group distilled water - Model control group distilled water - Experimental group 1 Example 1 Blood Activating, Stasis Removing and Symptom Removing Decoction 26g / kg (calculated as crude drug) Experimental Group 2 Example 2 Blood Activating, Stasis Removing and Symptom Eliminating Decoction 26g / kg (calculated as crude drug) Control group 1 Comparative Example 1 Chinese medicine composition 26g / kg (calculated as crude drug) Control group 2 Comparative Example 2 Chinese medicine composition 26g / kg (calculated as crude drug) Control group 3 Comparative Example 3 Chinese medicine composition 26g / kg (calculated as crude drug) Control group 4 Comparative Example 4: Shaofu Zhuyu Decoction 26g / kg (calculated as crude drug)

[0089] The drug was administered orally once daily for 7 consecutive days.

[0090] Determination of the number of abdominal contractions in rats

[0091] On day 7, 40 minutes after drug administration, 4 U of oxytocin was injected intraperitoneally per rat. The number of abdominal contractions within 15 minutes after oxytocin injection was recorded.

[0092] Determination of hemorheological parameters in rats

[0093] Twenty minutes after oxytocin injection, rats were anesthetized with 5 mL / kg of 4% chloral hydrate. Then, 5 mL of blood was collected from the abdominal aorta and placed into a heparinized tube. The tube was slowly inverted several times to prevent local coagulation. Three mL of blood was collected for determination of erythrocyte aggregation rate. The remaining 2 mL of blood was centrifuged at 3000 rpm for 5 minutes, and plasma was collected for determination of plasma viscosity.

[0094] Determination of rat uterine swelling

[0095] After blood was collected from the rats, the uterus on both sides was removed and quickly rinsed in ice-cold physiological saline to rinse away the blood. The uterus was then wiped dry with filter paper and weighed to calculate the degree of swelling (mg = weight of the inflamed side uterus - weight of the non-inflamed side uterus).

[0096] 2. Statistical methods

[0097] GraphPad Prism 7.0 software was used for data processing and the quantitative data were The comparison between groups was performed with one-way analysis of variance, and the comparison between two groups was performed with LSD-t test. The difference was considered statistically significant when P < 0.05.

[0098] 3. Experimental Results

[0099] 3.1 Effect on the number of abdominal contractions in rats

[0100] Intraperitoneal injection of oxytocin induces a complex pattern in rats, characterized by curling up into a ball, abdominal contraction, and the anterior abdominal wall pressing against the cage floor. One strong abdominal contraction is counted as one contraction.

[0101] Compared with the blank control group, the number of abdominal contractions in the model control group was significantly increased.

[0102] Compared with the model control group, the number of abdominal contractions in the rats in experimental group 1 and experimental group 2 was significantly reduced, indicating that the Huoxue Huayu Xiaozheng Decoction of the present invention can significantly relieve the pain of the model rats. Compared with the control groups 1-4, there was a significant difference in reducing the number of abdominal contractions in the rats in experimental group 1 and experimental group 2. Figure 1 .

[0103] 3.2 Effects on hemorheological parameters in rats

[0104] Compared with the blank control group, the plasma viscosity and red blood cell aggregation rate of rats in the model control group were significantly increased.

[0105] Compared with the model control group, the plasma viscosity and red blood cell aggregation rate of rats in Experimental Groups 1 and 2 were significantly reduced, indicating that the Huoxue Huayu Xiaozheng Decoction of the present invention can significantly alleviate uterine inflammation in model rats. Compared with Control Groups 1-4, Experimental Groups 1 and 2 showed significant differences in reducing plasma viscosity and red blood cell aggregation rate. The results are shown in Table 2.

[0106] Table 2 Comparison of blood rheology indicators in each group ( n=6)

[0107]

[0108] Note: Different lowercase letters in the same column indicate significant differences among different groups (P < 0.05).

[0109] 3.3 Effect on rat uterine swelling

[0110] Compared with the blank control group, the uterine swelling of rats in the model control group was significantly increased.

[0111] Compared with the model control group, the uterine swelling of rats in experimental group 1 and experimental group 2 was significantly reduced, indicating that the Huoxue Huayu Xiaozheng Decoction of the present invention can significantly reduce uterine inflammation in model rats. Compared with control groups 1-4, experimental group 1 and experimental group 2 showed significant differences in reducing uterine swelling in rats. Figure 2 .

[0112] Changing the components or compatibility ratios of the present invention's formula weakens the therapeutic effect of Huoxue Huayu Xiaozheng Decoction on dysmenorrhea secondary to endometriosis. The components of the present invention act synergistically. Huoxue Huayu Xiaozheng Decoction is superior to Shaofu Zhuyu Decoction in treating dysmenorrhea secondary to endometriosis.

[0113] The above embodiments do not limit the present invention in any form. For those skilled in the art, any modifications, replacements, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A blood-activating, blood-stasis-removing and syndrome-eliminating soup, characterized in that: The blood-activating, blood-stasis-removing and syndrome-eliminating soup comprises the following raw materials by weight: 15-25 parts of pollen, 15-25 parts of cinnamon bark, 15-25 parts of angelica sinensis, 12-18 parts of stir-fried white peony root, 15-25 parts of notoginseng, 12-18 parts of zedoaria, 15-25 parts of salvia miltiorrhiza, 10-15 parts of madder, 4-8 parts of liquorice, 15-25 parts of cyperus rotundus, 12-18 parts of selfheal, 15-20 parts of ligusticum chuanxiong and 8-12 parts of leech.

2. The blood-activating, blood-stasis-removing and syndrome-eliminating decoction according to claim 1, characterized in that: The blood-activating, blood-stasis-removing and syndrome-eliminating soup is prepared from the following components by weight: 20 parts of pollen, 20 parts of aconite root, 20 parts of angelica root, 15 parts of stir-fried white peony root, 20 parts of notoginseng, 15 parts of zedoaria, 20 parts of salvia miltiorrhiza, 12 parts of madder root, 6 parts of liquorice, 20 parts of cyperus rotundus, 15 parts of selfheal, 18 parts of ligusticum chuanxiong and 10 parts of leech.

3. The blood-activating, blood-stasis-removing and symptom-eliminating decoction according to claim 1, characterized in that: The blood-activating, blood-stasis-removing and symptom-eliminating soup is prepared from the following raw materials by weight: 15 parts of pollen typhae, 25 parts of aconite root, 15 parts of angelica root, 18 parts of stir-fried white peony root, 25 parts of notoginseng, 18 parts of zedoaria, 15 parts of salvia miltiorrhiza, 15 parts of madder root, 8 parts of liquorice, 25 parts of cyperus rotundus, 12 parts of selfheal, 20 parts of ligusticum chuanxiong and 8 parts of leech.

4. The blood-activating, blood-stasis-removing and syndrome-eliminating decoction according to claim 1, characterized in that: The blood-activating, blood-stasis-removing and symptom-eliminating soup is prepared from the following raw materials by weight: 25 parts of pollen typhae, 15 parts of aconite root, 25 parts of angelica root, 12 parts of stir-fried white peony root, 15 parts of notoginseng, 12 parts of zedoaria, 25 parts of salvia miltiorrhiza, 10 parts of madder root, 4 parts of liquorice, 15 parts of cyperus rotundus, 18 parts of selfheal, 15 parts of ligusticum chuanxiong and 12 parts of leech.

5. The method for preparing the Huoxue Huayu Xiaozheng Decoction according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: weighing raw materials according to a formula, mixing, adding water 6-8 times the weight of the mixture to the mixture, soaking for 0.5-1h, decocting the obtained soaking liquid to 95-100°C, cooling to 90-95°C and decocting for 0.5-1h, filtering to obtain a first decoction liquid and medicinal residues; adding water 4-6 times the weight of the medicinal residues to the medicinal residues, then boiling to 95-100°C, cooling to 90-95°C and decocting for 0.5-1h, filtering to obtain a second decoction liquid; and mixing the first decoction liquid and the second decoction liquid.

6. A Chinese medicine preparation of Huoxue Huayu Xiaozheng decoction, characterized in that: The traditional Chinese medicine preparation is prepared with the blood-activating, blood-stasis-removing and symptom-eliminating decoction according to any one of claims 1 to 4 as a raw material.

7. The Chinese medicine preparation of Huoxue Huayu Xiaozheng Decoction according to claim 6, characterized in that: The traditional Chinese medicine preparation also includes pharmaceutically acceptable excipients.

8. The Chinese medicine preparation of Huoxue Huayu Xiaozheng Decoction according to claim 6 or 7, characterized in that: The dosage forms of the traditional Chinese medicine preparation include granules, capsules, tablets, powders, pills or oral liquids.

9. Use of the Huoxue Huayu Xiaozheng Decoction according to any one of claims 1 to 4 in the preparation of a medicament for preventing or treating endometriosis, secondary dysmenorrhea, irregular menstruation, pelvic mass or ovarian cyst caused by blood stasis in the meridians and qi stagnation.

10. Use of the Huoxue Huayu Xiaozheng decoction according to any one of claims 1 to 4 in the preparation of a medicament for preventing or treating endometriosis or secondary dysmenorrhea.

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