Intelligent integrated biological safety double experiment module

Abstract

The invention provides an intelligent integrated biological safety double-experiment cabin. The intelligent integrated biological safety double-experiment cabin comprises a cabin body, the cabin body comprises a sample extraction cabin, an amplification sequencing negative pressure cabin and an isolation room; the sample extraction cabin is provided with a first air outlet duct and a first air inlet duct, and the first air outlet duct and the first air inlet duct form positive pressure in the sample extraction cabin; the amplification sequencing negative pressure cabin is provided with a second air outlet duct and a second air inlet duct, and the second air outlet duct and the second air inlet duct form negative pressure in the amplification sequencing negative pressure cabin; the first air outlet duct and the second air outlet duct are arranged in the isolation room, and the sample extraction cabin is isolated from the amplification sequencing negative pressure cabin through the isolation room. According to the invention, regional operation and management can be carried out based on different operation requirements, and the influence of air flow between different regions on a biological experiment environment is effectively avoided. Meanwhile, experimental equipment can be flexibly arranged according to experimental requirements, so that the overall space utilization rate is increased, and the cabin structure is stabilized.

Description

Technical Field

[0001] The present application belongs to the field of biosafety technology, and in particular relates to an intelligent integrated biosafety dual laboratory cabin. Background Art

[0002] In biological experiments, especially PCR experiments (gene amplification experiments), aerosols and other pollutants are easily generated, which can contaminate samples during the experiment, thus affecting the smooth progress of the biological experiment. In addition, during the operation, the door of the experimental chamber needs to be opened, at which time outside air can easily enter the chamber, which will also affect the detection process of biological samples and thus affect the accuracy of the experimental results. In addition, when the operating chamber is opened for loading, these pollutants are also easy to escape outward, thereby polluting personnel and the environment, resulting in lower safety of the detection operation. Summary of the Invention

[0003] The present application provides an intelligent integrated biosafety double experiment chamber to solve the technical problem of how to improve experimental accuracy and safety and improve space utilization.

[0004] In the first aspect, the present application provides an intelligent integrated biosafety double experiment cabin, comprising a cabin body; the cabin body comprises a sample extraction cabin, an amplification sequencing negative pressure cabin and an isolation room; the sample extraction cabin is provided with a first air outlet and a first air inlet, and the first air outlet and the first air inlet form a positive pressure in the sample extraction cabin; the amplification sequencing negative pressure cabin is provided with a second air outlet and a second air inlet, and the second air outlet and the second air inlet form a negative pressure in the amplification sequencing negative pressure cabin; the first air outlet and the second air outlet are provided in the isolation room, and the sample extraction cabin and the amplification sequencing negative pressure cabin are isolated by the isolation room.

[0005] In an implementation of the first aspect, the cabin includes two layers of space, namely a first layer and a second layer; the sample extraction cabin, the amplification sequencing negative pressure cabin and the isolation room are located in the second layer; the first air inlet duct and the second air inlet duct are located in the first layer.

[0006] In an implementation of the first aspect, the cabin body includes three layers of space, namely, a first layer of space, a second layer of space, and a third layer of space; the sample extraction cabin, the amplification sequencing negative pressure cabin, and the isolation room are located in the second layer of space; the first air inlet duct and the second air inlet duct are located in the first layer of space; a sample extraction device set is provided in the sample extraction cabin, and an amplification sequencing device set is provided in the amplification sequencing negative pressure cabin; the centrifuge and / or the conveying mechanism in the sample extraction device set are located in the third layer of space.

[0007] In an implementation of the first aspect, a first purification device is provided in the first layer of space above the sample extraction cabin, and the first purification device is connected to the air inlet of the first air inlet duct.

[0008] In an implementation of the first aspect, a second purification device is provided in the first layer of space above the amplification sequencing negative pressure cabin, and the second purification device is connected to the air inlet of the second air inlet duct.

[0009] In an implementation of the first aspect, the air outlet of the first air outlet duct and the air outlet of the second air outlet duct both extend from the top of the isolation room to the outside of the cabin.

[0010] In an implementation of the first aspect, a delivery port is provided in the second space, and a microplate containing samples and / or reagents moves between the second space and the third space through the delivery port.

[0011] In an implementation of the first aspect, a clamp is provided in the second-layer space, and the clamp includes a clamping claw and a telescopic rod. The clamping claw is installed at one end of the telescopic rod, and the clamping claw passes through the delivery port through the telescopic rod to transport the clamped microplate containing samples and / or reagents from the second-layer space to the third-layer space, or transport the clamped microplate containing samples and / or reagents from the third-layer space to the second-layer space.

[0012] In an implementation of the first aspect, a clamp is provided in the second layer space, and the clamp is assembled with a moving device provided in the second layer space so that the clamp moves along a first direction and / or a second direction, and the first direction and the second direction are perpendicular to each other.

[0013] In an implementation of the first aspect, the moving device includes a first moving structure, a second moving structure and a third moving structure, the first moving structure and the second moving structure are perpendicular to each other, the third moving mechanism is parallel to the second moving mechanism, the first moving mechanism is assembled with the second moving mechanism and the third moving mechanism respectively so that the first moving mechanism moves along the first direction, and the clamp is assembled with the first moving mechanism so that the clamp moves along the second direction.

[0014] The intelligent integrated biosafety dual-experimental chamber described in this application has the following beneficial effects: It can provide regional operations and management based on different operational requirements, effectively preventing the impact of air flow between different areas on the biological experimental environment. It can also prevent the leakage of pollutants from negatively impacting the experimental environment and experimenters. Furthermore, it can flexibly arrange experimental equipment according to experimental needs, thereby improving overall space utilization and stabilizing the chamber structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Shown is a structural schematic diagram of an intelligent integrated biosafety dual laboratory chamber described in one embodiment of the present application.

[0016] Figure 2 Shown is a structural schematic diagram of an intelligent integrated biosafety dual laboratory chamber described in one embodiment of the present application.

[0017] Figure 3 Shown is a structural schematic diagram of an intelligent integrated biosafety dual laboratory chamber described in one embodiment of the present application.

[0018] Figure 4 Shown is a schematic diagram of the installation of the delivery port in the second layer space according to an embodiment of the present application.

[0019] Figure 5 Shown is a schematic diagram of the installation of the transmission mechanism in the third space according to an embodiment of the present application.

[0020] Figure 6 Shown is a schematic diagram of the installation of the transmission mechanism in the third space according to an embodiment of the present application.

[0021] Figure 7 Shown is a schematic assembly diagram of a mobile device according to an embodiment of the present application.

[0022] Figure 8 Shown is a schematic diagram of the assembly of a mobile device according to an embodiment of the present application.

[0023] Figure 9 Shown is a schematic assembly diagram of a mobile device according to an embodiment of the present application.

[0024] Figure 10 Shown is a top view schematic diagram of a second layer space according to an embodiment of the present application.

[0025] Figure 11 Structural design diagram of a fully automatic robotic arm provided in an embodiment of the present application

[0026] Component number description

[0027] A cabin

[0028] A1 First floor space

[0029] A11 conveyor port

[0030] A2 Second Floor Space

[0031] A3 Third Floor Space

[0032] A11 conveyor port

[0033] A31 centrifuge

[0034] 1 Sample extraction chamber

[0035] 2 Amplification Sequencing Negative Pressure Chamber

[0036] 3 Isolation Room

[0037] 4. Fixture

[0038] 5 Mobile devices

[0039] 51 First moving mechanism

[0040] 52 Second moving mechanism

[0041] 53 Third moving mechanism

[0042] 6 Display Devices

[0043] 7 Transmission mechanism

[0044] 71-72 transfer window

[0045] 10 fully automatic robotic arms DETAILED DESCRIPTION

[0046] The following describes the embodiments of the present application through specific examples. Those skilled in the art can easily understand the other advantages and effects of the present application from the content disclosed in this specification. The present application can also be implemented or applied through other different specific embodiments. The details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present application. It should be noted that the following embodiments and features in the embodiments can be combined with each other unless they conflict.

[0047] It should be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present application. Therefore, the illustrations only show components related to the present application and are not drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component can be changed at will, and the component layout type may also be more complicated.

[0048] In addition, the descriptions of "first", "second", etc. in this application are for descriptive purposes only and should not be understood as indicating or implying their relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions between the various embodiments can be combined with each other, but this must be based on the fact that they can be implemented by ordinary technicians in this field. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by this application.

[0049] In biological experiments, especially PCR experiments (gene amplification experiments), aerosols and other pollutants are easily generated. Existing technologies cannot effectively prevent the leakage of pollutants, which has an adverse effect on the accuracy and safety of the experiment. At the same time, since various large-scale equipment are installed on the operating table, the stability of the laboratory cabin and the convenience of the experiment are affected. In addition, when working in different areas, it is necessary to constantly open doors and windows, which leads to the leakage of pollutants during the experiment and the communication of gases between different working areas, thereby affecting the stability and safety of the experimental environment.

[0050] In order to at least solve the above problems, an embodiment of the present application provides an intelligent integrated biosafety double laboratory cabin that can perform zoned operations and management, avoid air circulation contamination between different areas, and improve space utilization.

[0051] See also Figures 1 to 2 As shown, an intelligent integrated biosafety double experimental cabin provided by an embodiment of the present application includes a cabin body A; the cabin body A includes a sample extraction cabin 1, an amplification sequencing negative pressure cabin 2 and an isolation room 3; the sample extraction cabin 1 is provided with a first air outlet and a first air inlet, and the first air outlet and the first air inlet form a positive pressure in the sample extraction cabin 1; the amplification sequencing negative pressure cabin 2 is provided with a second air outlet and a second air inlet, and the second air outlet and the second air inlet form a negative pressure in the amplification sequencing negative pressure cabin 2; the first air outlet and the second air outlet are arranged in the isolation room 3, and the sample extraction cabin 1 and the amplification sequencing negative pressure cabin 2 are isolated by the isolation room 3.

[0052] It should be noted that the positive pressure in the sample extraction chamber 1 refers to the air pressure inside the chamber being greater than the air pressure outside the chamber. This prevents air outside the chamber from flowing into the chamber through gaps or when the chamber door is opened. The negative pressure in the amplification sequencing negative pressure chamber 2 primarily refers to the air pressure inside the chamber being less than the air pressure outside the chamber. This prevents contaminants generated inside the chamber from flowing out through gaps or when the chamber door is opened.

[0053] In some embodiments, the first air inlet duct includes a first air inlet fan, and the second air inlet duct includes a second air inlet fan. The first air inlet fan can actively input external air into the sample extraction cabin 1, and the second air inlet fan can actively input external air into the amplification sequencing negative pressure cabin 2. The first air outlet duct includes a first exhaust fan, and the second air outlet duct includes a second exhaust fan. The first exhaust fan can actively extract the gas in the sample extraction cabin 1 and discharge it outside the cabin through the first air outlet duct, and the second air inlet fan can actively extract the gas in the amplification sequencing negative pressure cabin 2 and discharge it outside the cabin through the second air outlet duct. In this way, a positive pressure can be formed in the sample extraction cabin 1 through the cooperation between the first air inlet duct and the first air outlet duct, thereby preventing the air outside the cabin from flowing into the cabin through gaps or when the cabin door is open during the sample extraction work and causing contamination to the biological samples, thereby improving the accuracy of the detection. At the same time, negative pressure is formed in the amplification sequencing negative pressure chamber 2 through the second air outlet duct and the second air inlet duct, thereby preventing pollutants generated during the amplification process from being released outward through gaps or cabin doors, thereby preventing the surrounding environment from being polluted, especially toxic gases that may affect the personal health of on-site workers, thereby improving the safety of detection operations.

[0054] Furthermore, both the first and second air outlets are located within the isolation chamber 3, with their outlets extending from the top of the isolation chamber 3 to the exterior of the chamber A. By discharging air from the chamber through a pipe from the top of the isolation chamber 3 to a designated area, the safety of the experimental environment and personnel is further ensured. Furthermore, by isolating the sample extraction chamber 1 and the amplification and sequencing negative pressure chamber 2 through the isolation chamber 3, the extraction and testing processes can be separated into separate zones, thereby managing air pressure in different work areas and preventing air flow between different zones, further enhancing the safety of biological experiments.

[0055] In some embodiments, the sample extraction chamber 1 is provided with a sample extraction device set, and the amplification sequencing negative pressure chamber 2 is provided with an amplification sequencing device set. The sample extraction device set includes a sample lysis device, a sample purification device, a sample or / and reagent transfer device, a centrifuge, a clamp, a moving device, a pipette, a sample separation device, etc.; the amplification sequencing device includes a PCR instrument, a sample or / and reagent transfer device, a gene sequencing device, etc. This application does not impose any restrictions on the specific equipment layout and selection. Taking the above-mentioned experimental equipment as an example, please continue to refer to Figures 1 to 9 The biological experiment process of the intelligent integrated biosafety experiment chamber provided by the embodiment of the present application includes:

[0056] First, reagents are prepared in the sample extraction chamber 1. Reagents are packaged in the sample extraction chamber 1 using a pipette. Afterwards, the biological sample is extracted using a cotton swab and placed in a sample plate, which includes two layers of tube racks, wherein the test tubes on the upper tube rack are shorter and the test tubes on the lower tube rack are longer. The test tube openings on the two tube racks correspond to each other. When the two tube racks are connected, the test tubes on the upper tube rack are inserted into the corresponding test tubes on the lower tube rack, thereby completing the sample preparation. At this time, the sample plate is clamped by a clamp and can be moved in a first direction and / or a second direction by a moving device, and the first direction and the second direction are perpendicular to each other. The moving device sends the sample plate into a centrifuge for cell rupture and separation, and throws the liquid on the tube wall and tube cover to the bottom of the test tube in the lower tube rack. Afterwards, the cotton swabs and other tools still remaining in the upper tube rack are discarded. The clamp continues to clamp the biological sample in the test tube of the lower tube rack for purification and cooling. Afterwards, the sample is moved from the sample extraction chamber 1 to the amplification sequencing negative pressure chamber 2 by a conveyor mechanism for amplification by a PCR instrument. Finally, the PCR amplification product is sent to the biological sample testing equipment in the amplification sequencing negative pressure chamber 2 by a fully automatic robot for amplification analysis.

[0057] The experimental equipment that must be designed in the experimental cabin will be further explained below.

[0058] In some embodiments, the cabin A includes three layers of space, namely, the first layer space A1, the second layer space A2 and the third layer space A3; the sample extraction cabin 1, the amplification sequencing negative pressure cabin 2 and the isolation room 3 are located in the second layer space A2; the first air inlet duct and the second air inlet duct are located in the first layer space A1; the centrifuge and the conveying mechanism are located in the third layer space A3. By performing layered space management on the cabin A, different space layers realize different functions, thereby further improving space utilization. At the same time, if Figure 3 As shown, installing large equipment such as centrifuge A31 in the third space, that is, the bottom of the cabin, can better stabilize the cabin structure. Figure 4 and Figure 6 The biological sample can be moved from the second space A2 to the third space A3 through the delivery port A11 to be separated by the centrifuge A31.

[0059] Please refer to Figure 3 The third-layer space, A3, is a frame structure constructed from multiple aluminum profiles. Large equipment like centrifuges or transmission mechanisms can be installed within the frame gaps. This allows for easy access to equipment within the third-layer space for maintenance when necessary, fully utilizing the gaps within the frame without occupying additional space within the test chamber, thus improving space efficiency.

[0060] It should be noted that the transport mechanism is used to transport the extracted biological sample from the sample extraction chamber 1 to the amplification sequencing negative pressure chamber 2. Figure 4 and Figure 6 In some embodiments, both the sample extraction chamber 1 and the amplification sequencing negative pressure chamber 2 of the second space A2 are provided with a delivery port. The biological sample is delivered from the second space A2 to the third space A3 through the delivery port A11. The biological sample is then delivered from the third space A3 below the sample extraction chamber 1 to the third space A3 below the amplification sequencing negative pressure chamber 2 via a delivery mechanism 7. Regarding the specific type of delivery mechanism 7, it can be a conveyor belt, a motor, or other delivery device, and this application is not limited thereto.

[0061] In some embodiments, a first purifier 11 is provided in the first space A1 above the sample extraction chamber 1. The first purifier 11 is connected to the air inlet of the first air inlet duct. A second purifier 21 is provided in the first space A1 above the amplification sequencing negative pressure chamber 2. The second purifier 51 is connected to the air inlet of the second air inlet duct. The first purifier 11 and the second purifier 21 filter the outside air to prevent pollutants in the outside air from contaminating the biological samples. At the same time, clean air circulation can be effectively achieved, further ensuring the cleanliness of the experimental environment.

[0062] In some embodiments, a first air pressure sensor is provided in the sample extraction cabin 1, so as to utilize the first air pressure sensor to detect the air pressure in the sample extraction cabin 1 in real time, and the first air pressure sensor is communicatively connected to the PLC controller, and the PLC controller is communicatively connected to the exhaust fan of the first air outlet duct. The first air pressure sensor will feed back the detected air pressure information to the PLC controller, and the PLC controller will control the working state of the exhaust fan according to the received air pressure information in the sample extraction cabin 1. If the first air pressure sensor measures that the air pressure in the sample extraction cabin 1 is relatively high, resulting in the positive pressure formed by the air pressure in the sample extraction cabin 1 compared with the external air pressure exceeding the set range, the PLC controller will control the exhaust fan to start or increase the power of the exhaust fan until the air pressure in the sample extraction cabin 1 reaches the set requirements, and the PLC controller will then control the exhaust fan to stop, or run at the corresponding power.

[0063] In some embodiments, a second air pressure sensor is provided in the amplification sequencing negative pressure chamber 2 to detect the air pressure within the amplification sequencing negative pressure chamber 2 in real time. The second air pressure sensor is in communication with a PLC controller, which in turn is in communication with the air intake fan. The second air pressure sensor feeds back the detected air pressure information to the PLC controller, which then controls the operating state of the air intake fan based on the received air pressure information within the amplification sequencing negative pressure chamber 2. If the second air pressure sensor detects that the air pressure within the amplification sequencing negative pressure chamber 2 is low, resulting in the pressure within the amplification sequencing negative pressure chamber 2 being lower than a set pressure value, the PLC controller will control the air intake fan to start or increase its power until the air pressure within the amplification sequencing negative pressure chamber 2 reaches the set pressure value. The PLC controller will then control the air intake fan to stop or operate at a corresponding power level.

[0064] Specifically, a clamp 4 is provided in the second space A2. The clamp 4 includes a clamping claw and a telescopic rod. The clamping claw is mounted on one end of the telescopic rod. The clamping claw passes through the delivery port via the telescopic rod to move the clamped microplate containing samples and / or reagents between the second space A2 and the third space A3. Figure 4 As shown, a transfer port A11 is provided in the second space for transferring a microplate containing samples and / or reagents from the second space A2 to the third space A2, or from the third space A2 to the second space A2.

[0065] In some embodiments, a transmission mechanism 7 is provided in the third space A3. Figure 5 and Figure 6 As shown, the transfer mechanism 7 includes a loading platform and two transfer windows 71-72. The loading platform can move from one transfer window to the other. The two transfer windows are respectively located in the third layer space A3 below the sample extraction chamber 1 and below the amplification sequencing negative pressure chamber 2 in the third layer space A3, thereby realizing the transfer of biological samples on the loading platform. Furthermore, the transfer windows 71~72 can be blocked by a baffle. When the baffle is removed from the transfer window, the stage is connected to the sample extraction chamber 1 or the amplification sequencing negative pressure chamber 2 through the transfer window, so that the microplate containing samples and / or reagents can be clamped by a clamp and moved from the second layer space A2 to the third layer space A3 through the transfer window 71 located below the sample extraction chamber 1, and placed on the stage. The stage moves from the transfer window to the transfer window 72 located below the amplification sequencing negative pressure chamber 2. At this time, the baffle is removed, and the clamp in the amplification sequencing negative pressure chamber 2 extends downward, clamps the biological sample from the transfer window 72, and returns to the amplification sequencing negative pressure chamber 2 for amplification and detection processes.

[0066] The coordination of the fixture 4 and the transport mechanism effectively prevents the chambers from direct contact with the outside air, potentially affecting the air quality inside the sample extraction chamber 1 and the amplification and sequencing negative pressure chamber 2, as well as the outside air quality. This prevents any impact on the corresponding experimental procedures, thereby improving detection accuracy, avoiding any impact on the on-site environment, and enhancing on-site operation safety. Furthermore, it effectively prevents air flow between the sample extraction chamber 1 and the amplification and sequencing negative pressure chamber 2, thereby ensuring that the two chambers do not affect each other, thereby further improving detection accuracy.

[0067] See also Figures 7 to 9 As shown, the clamp 4 is assembled with a moving device 5 disposed within the second space to enable the clamp 4 to move in the first direction and / or the second direction. Depending on the needs of biological experiments, different experimental equipment is often installed to extract biological samples. In this case, the clamp 4 is required to enable the free movement of the biological sample so that it can be placed on different experimental equipment.

[0068] Please continue reading Figures 7 to 9 The mobile device 5 includes a first mobile structure 51, a second mobile structure 52, and a third mobile structure 53. The third mobile structure 53 is fixed to the operating table. The first mobile structure 51 and the second mobile structure 52 are arranged above the table. The first mobile structure 51 and the second mobile structure 52 are perpendicular to each other. The third mobile structure 53 is parallel to the second mobile structure 52. The first mobile structure 51 is assembled with the second mobile structure 52 and the third mobile structure 53 respectively so that the first mobile structure 51 moves in the first direction. The clamp 4 is assembled with the first mobile structure 51 so that the clamp 4 moves in the second direction. In some embodiments, the first mobile structure 51 and the third mobile structure 53 are connected by a support column perpendicular to the table. The support column is connected to the mobile unit on the third mobile structure 53. The other end of the first mobile structure 51 is connected to the mobile unit of the second mobile structure 52, so that the first mobile structure 51 can move in the first direction. In addition, the clamp 4 is fixed to the mobile unit on the first mobile structure 51, so that the clamp can be moved in the second direction through the first mobile structure 51.

[0069] In some embodiments, a reference coordinate system is provided within the chamber, wherein the first direction is the X direction and the second direction is the Y direction, i.e., the first and second directions are perpendicular to each other. In other words, the clamp 4 can be moved in the X direction via the first movable unit 51, while the first movable unit 51 can be moved in the Y direction via the second movable unit 52 and the third movable unit 53. Furthermore, as mentioned above, the clamp 4 can also be freely extended and retracted to achieve Z-direction movement, thereby adapting to different spatial layers (from the second space A2 to the third space A3 or from the third space A3 to the second space A2) and the different heights of experimental equipment within the layer, thereby achieving precise sample capture and movement.

[0070] In other embodiments, the cabin A includes two layers of space, namely the first layer and the second layer; the sample extraction cabin 1, the amplification sequencing negative pressure cabin 2 and the isolation room 3 are located in the second layer; the first air inlet duct and the second air inlet duct are located in the first layer.

[0071] like Figure 10 The figure shows a top view of the second space A2. In some embodiments, the amplification and sequencing negative pressure chamber 2 of the second space A2 can simultaneously realize the functions of amplification and analysis of amplification products. Figure 8 When the biological sample is moved into the negative pressure chamber 2 for amplification and sequencing, it undergoes amplification. A fully automated robot then delivers the amplified product to a testing device for testing. The term "fully automated robot" primarily refers to automated equipment that moves the amplified product, and does not restrict the specific type or model of the automated equipment.

[0072] Figure 11 This is a structural design diagram of a fully automatic robot arm 10 provided in an embodiment of the present application, as shown in FIG. Figure 11 As shown, the fully automatic robot arm 10 includes a gripper, moving parts, branches, etc.

[0073] It should be noted that the specific experimental equipment on the operating table in the sample extraction chamber 1 and the amplification sequencing negative pressure chamber 2 in the second layer space A2 can be freely selected according to experimental requirements, and this application does not impose any restrictions on this. The experimental chamber provided in this application can meet the needs of different experimental layouts and meet the needs of the experimental environment by dividing the experimental area, further improving the safety and convenience of biological experiments.

[0074] like Figure 1As shown, the second space A2 is also equipped with a display device 6. This display device 6 comprises a screen that can be used to display the test results of biological samples within the amplification sequencing negative pressure chamber 2. In this case, an automated program can automatically read and store the experimental results, eliminating the need for manual recording, further reducing experimental costs. Furthermore, the experimental environment within the chamber A can be controlled directly through the display device 6, eliminating the need to manually adjust the experimental environment within the chamber, achieving comprehensive automated control. This application is not limited to the specific type of display device 6.

[0075] In some embodiments, the first space A1 may also be equipped with ultraviolet disinfection equipment, monitoring equipment, and lighting equipment to further disinfect and monitor the experimental environment within the chamber and enhance the safety of biological experiments. This application is not limited to the specific types and models of ultraviolet disinfection equipment, monitoring equipment, and lighting equipment.

[0076] As described above, the intelligent integrated biosafety dual laboratory chamber provided by this application can provide regional operations and management based on different operational requirements, effectively avoiding the impact of air flow between different areas on the biological experimental environment. At the same time, this application can also prevent the leakage of pollutants from negatively impacting the experimental environment and experimental personnel. In addition, this application can flexibly arrange experimental equipment according to experimental needs, thereby improving overall space utilization and stabilizing the cabin structure.

[0077] The descriptions of the processes or structures corresponding to the above figures have different emphases. For parts that are not described in detail in a certain process or structure, please refer to the relevant descriptions of other processes or structures.

[0078] The above embodiments are merely illustrative of the principles and effects of this application and are not intended to limit this application. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of this application. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical concepts disclosed in this application shall be covered by the claims of this application.

Claims

1. An intelligent integrated biosafety double laboratory cabin, characterized by: Including cabin; The cabin includes a sample extraction cabin, an amplification sequencing negative pressure cabin, and an isolation room; the sample extraction cabin is provided with a first air outlet and a first air inlet, and the first air outlet and the first air inlet form a positive pressure in the sample extraction cabin; the amplification sequencing negative pressure cabin is provided with a second air outlet and a second air inlet, and the second air outlet and the second air inlet form a negative pressure in the amplification sequencing negative pressure cabin; The first air outlet duct and the second air outlet duct are arranged in the isolation room, and the sample extraction cabin and the amplification sequencing negative pressure cabin are isolated by the isolation room.

2. The intelligent integrated biosafety double laboratory chamber according to claim 1 is characterized in that: The cabin body includes two layers of space, namely the first layer and the second layer; the sample extraction cabin, the amplification sequencing negative pressure cabin and the isolation room are located in the second layer; the first air inlet duct and the second air inlet duct are located in the first layer.

3. The intelligent integrated biosafety double laboratory chamber according to claim 1 is characterized in that: The cabin body includes three layers of space, namely the first layer, the second layer and the third layer; the sample extraction cabin, the amplification sequencing negative pressure cabin and the isolation room are located in the second layer; the first air inlet duct and the second air inlet duct are located in the first layer; a sample extraction device set is provided in the sample extraction cabin, and an amplification sequencing device set is provided in the amplification sequencing negative pressure cabin; the centrifuge and / or the conveying mechanism in the sample extraction device set are located in the third layer.

4. The intelligent integrated biosafety dual experiment chamber according to claim 2 or 3, characterized in that: A first purification device is provided above the sample extraction cabin in the first layer of space, and the first purification device is connected to the air inlet of the first air inlet duct.

5. The intelligent integrated biosafety dual experiment chamber according to claim 2 or 3, characterized in that: A second purification device is provided in the first layer of space above the amplification sequencing negative pressure cabin, and the second purification device is connected to the air inlet of the second air inlet duct.

6. The intelligent integrated biosafety double laboratory chamber according to claim 1 is characterized in that: The air outlet of the first air outlet duct and the air outlet of the second air outlet duct both extend from the top of the isolation room to the outside of the cabin.

7. The intelligent integrated biosafety double laboratory chamber according to claim 3 is characterized in that: A delivery port is provided in the second space, and the microplate containing samples and / or reagents moves between the second space and the third space through the delivery port.

8. The intelligent integrated biosafety double laboratory chamber according to claim 7 is characterized in that: A clamp is provided in the second layer space, and the clamp includes a clamping claw and a telescopic rod. The clamping claw is installed at one end of the telescopic rod. The clamping claw passes through the delivery port through the telescopic rod to transport the clamped microporous plate containing samples and / or reagents from the second layer space to the third layer space, or to transport the clamped microporous plate containing samples and / or reagents from the third layer space to the second layer space.

9. The intelligent integrated biosafety dual experiment chamber according to claim 2 or 3, characterized in that: A clamp is provided in the second space, and the clamp is assembled with a moving device provided in the second space so that the clamp moves along a first direction and / or a second direction, wherein the first direction and the second direction are perpendicular to each other.

10. The intelligent integrated biosafety double laboratory chamber according to claim 9 is characterized in that: The moving device includes a first moving structure, a second moving structure and a third moving structure. The first moving structure and the second moving structure are perpendicular to each other, and the third moving structure is parallel to the second moving structure. The first moving structure is assembled with the second moving structure and the third moving structure respectively so that the first moving structure moves along the first direction, and the clamp is assembled with the first moving structure so that the clamp moves along the second direction.

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