Method for extracting peony seed oil from peony seed meal

Abstract

The invention relates to the technical field of peony seed oil extraction, and particularly discloses a method for extracting peony seed oil from peony seed meal. The peony seed meal is crushed and then is steamed and fried, so that the cell structure is effectively destroyed, and the release of grease is facilitated; according to the method, enzymolysis treatment is performed after quenching and tempering treatment, besides conventional cellulase and pectinase, compound application of alpha-amylase and beta-amylase is adopted in a breakthrough mode for the treated compound enzyme, an excellent synergistic effect is shown, the alpha-amylase firstly acts on the outer surfaces of starch granules to decompose the starch granules into small polysaccharide fragments, and the beta-amylase acts on the outer surfaces of the starch granules to decompose the starch granules into small polysaccharide fragments; and beta-amylase further acts on the tail ends of the polysaccharide fragments to decompose the polysaccharide fragments into monosaccharides, so that the starch decomposition process is obviously accelerated, the enzymolysis efficiency is improved, and the rapid release of the grease is realized.

Description

Technical Field

[0001] The invention relates to the technical field of peony seed oil extraction, in particular to a method for extracting peony seed oil from peony seed meal. Background Art

[0002] Peony seed oil is a vegetable oil extracted from the seeds of peony flowers. Its rich nutrients and unique effects make it widely used in many fields.

[0003] Due to the high nutritional value of peony seed oil, the cultivation of oil-producing peony seeds in my country has been increasing year by year, and so has the production of these seeds. Based on a 30% oil yield from oil-producing peony seeds, the amount of peony seed meal produced after oil extraction is also very large, making its subsequent development and utilization a significant and challenging research topic.

[0004] In the existing technology, due to the limitations of extraction technology, the oil in peony seeds cannot be completely extracted. Therefore, a considerable amount of nutritional active ingredients, especially protein and unsaturated fatty acids, are still retained in the peony seed meal, making its recovery and treatment of great significance. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art, the present invention provides a method for extracting peony seed oil from peony seed meal, which is green, environmentally friendly, energy-saving, efficient and has low production cost.

[0006] The present invention is achieved through the following technical solutions: A method for extracting peony seed oil from peony seed meal, taking peony seed meal as a processing object, comprises the following steps: (1) The peony seed meal is crushed, then sprayed with water and stirred until it is moist, and then placed in a steaming and frying device and steamed and fried at 100-110°C; (2) adding water to the steamed and fried peony seed meal to prepare a suspension, adjusting the pH value of the suspension with protease, and then adding a complex enzyme consisting of cellulase, pectinase, α-amylase and β-amylase for enzymatic hydrolysis; (3) Centrifuging the enzymatic hydrolyzate, collecting the upper oil phase and the lower aqueous phase separately, and washing the bottom filter residue with water. The filtrate after washing is mixed with the lower aqueous phase to obtain a mixed aqueous phase; (4) placing the mixed aqueous phase in a reactor and heating it to 60-70°C, then performing ultrasonic demulsification treatment, allowing the demulsified mixed aqueous phase to stand, separating the upper oil phase, and mixing it with the oil phase obtained after enzymatic hydrolysis to obtain extracted peony seed oil; (5) The peony seed oil is refined to obtain the final product.

[0007] The present invention aims to solve the problem that residual oil still exists in peony seed meal after oil extraction, and re-processes the peony seed meal to extract the residual peony seed oil, thereby achieving the purpose of reducing the extraction cost of the peony seed oil. Since the peony seed meal itself has a low oil content, a low-cost, mild and energy-saving enzymatic hydrolysis method is selected for processing. In view of the defect of low efficiency of the enzymatic hydrolysis method, the composition of the composite enzyme is improved, and the peony seed meal is subjected to a conditioning pre-treatment, so as to improve the enzymatic hydrolysis efficiency.

[0008] The present invention crushes peony seed meal and then steams and stir-fries it, effectively destroying the cell structure and facilitating the release of oil. After the conditioning treatment, the meal is subjected to enzymatic hydrolysis treatment. In addition to conventional cellulase and pectinase, the composite enzyme used in the treatment breakthroughly adopts the composite application of alpha-amylase and beta-amylase, showing excellent synergistic effect. The alpha-amylase first acts on the outer surface of starch granules to decompose them into smaller polysaccharide fragments, and the beta-amylase further acts on the ends of the polysaccharide fragments to decompose them into monosaccharides, thereby significantly accelerating the starch decomposition process, improving the enzymatic hydrolysis efficiency, and realizing the rapid release of oil.

[0009] A better technical solution of the present invention is: In step (1), the peony seed meal is crushed and then passed through a 40-mesh sieve, the amount of spray water is 10-15% of the mass of the peony seed meal, and the meal is steamed and fried at 100-110° C. for 10-15 minutes, and mechanical crushing is combined with conditioning treatment to achieve cell structure destruction.

[0010] In step (2), the steamed and fried peony seed meal is mixed with water in a mass ratio of 1:4-5, the pH value of the suspension is adjusted to 7.0-8.0 by using protease, the amount of complex enzyme used is 0.8-1.4% of the mass of the peony seed meal, and enzymolysis is carried out at 45-55°C for 2-3 hours.

[0011] Further preferably, in the complex enzyme, the mass ratio of cellulase, pectinase, α-amylase and β-amylase is 2:2:1:1; after adjusting the combination of the complex enzyme, the performance of the enzymes used is improved and the cost is relatively reduced.

[0012] The temperature of the enzymatic extraction process does not exceed 70°C, the reaction conditions are mild, the oil yield is high, the nutrients are not destroyed, the production energy consumption is low, and it is not easy to cause environmental pollution.

[0013] In step (3), the enzymatic hydrolyzate is centrifuged at 4000-6000 rpm for 6-10 min, and the bottom filter residue is washed with water 2-3 times; after collecting the upper oil phase, i.e., peony seed oil, the lower aqueous phase (including the emulsified layer and the hydrolyzate) is further collected, and the bottom filter residue is washed with water to further collect the aqueous phase, so as to facilitate the subsequent separation of oil and fat in the aqueous phase.

[0014] In step (4), the ultrasonic demulsification process of the mixed aqueous phase is carried out at a high-low frequency combination of 20-24 kHz and 30-38 kHz, with an ultrasonic sound intensity of 1.2-1.8 W / cm and an ultrasonic time of 15-20 min.

[0015] Further preferably, the high and low frequency combination of the mixed aqueous phase ultrasonic treatment is: 20KHz, 30KHz; 22KHz, 34KHz or 24KHz, 38KHz.

[0016] In this area, ultrasonic demulsification and ultrasonic emulsification are a dynamic equilibrium process, which must pass through a certain process time. If the demulsification time is not satisfied, the water-oil separation is not thorough. Once the demulsification time is exceeded, it can only cause unnecessary energy consumption. If the demulsification time is too long, it will cause the oil-water two-phase to re-emulsify. The present invention is directed to the situation where the peony seed oil content in the peony seed meal recovery process is lower than that of conventional peony seed oil extraction. The demulsification method adopts a high-low frequency combination, designs suitable ultrasonic sound intensity and time, improves the water-oil separation effect in the recovered water phase, and increases the recovery amount of peony seed oil.

[0017] The invention is simple to operate, does not require complicated equipment, and is easy to realize industrial production. The oil in the peony seed meal is fully released through enzymatic hydrolysis, and the oil yield is high. The extraction conditions are mild, which reduces the oxidation and deterioration of the oil, and the oil quality is good, and the production cost is low. The hydrolyzate and residue after extraction can be further comprehensively utilized, which is green and environmentally friendly. DETAILED DESCRIPTION

[0018] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, the following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention may be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used in this specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0020] Example 1: A method for extracting peony seed oil from peony seed meal This embodiment specifically includes the following steps: (1) Using peony seed meal, a by-product of peony seed oil extraction, as raw material, 100 kg of peony seed meal was crushed and passed through a 40-mesh sieve, then sprayed with water and stirred until it was moist. The amount of spraying water was 10 kg of the mass of the peony seed meal. The meal was then placed in a steaming and frying device and steamed and fried at 100 °C for 15 min. (2) Add 400 kg of water to the steamed and fried peony seed meal to prepare a suspension, adjust the pH value of the suspension to 7.0 ± 0.2 with protease, then add 800 g of a complex enzyme consisting of cellulase, pectinase, α-amylase and β-amylase, mix well and perform enzymolysis at 45 °C for 3 hours; Among them, the complex enzymes include 267g of cellulase, 267g of pectinase, 133g of α-amylase, and 133g of β-amylase; (3) The enzymatic hydrolysate was centrifuged at 4000 rpm for 10 min, the upper oil phase and the lower aqueous phase were collected separately, and the bottom filter residue was washed twice with water. The filtrate after washing was mixed with the lower aqueous phase to obtain a mixed aqueous phase; (4) The mixed aqueous phase was placed in a reactor and heated to 60°C, and then subjected to ultrasonic demulsification treatment at a high-low frequency combination of 20 kHz and 30 kHz, with an ultrasonic sound intensity of 1.2 W / cm and an ultrasonic time of 20 min. The demulsified mixed aqueous phase was allowed to stand, and the upper oil phase was separated and mixed with the oil phase obtained after enzymatic hydrolysis to obtain the extracted peony seed oil; (5) The peony seed oil is refined to obtain the final product.

[0021] The weight of the peony seed oil extracted in this example is 8.41 kg. The aqueous phase recovered in the final treatment system can be used as reclaimed water for other industries after detoxification, and the residue can be used for organic fertilizer or feed processing.

[0022] Example 2: A method for extracting peony seed oil from peony seed meal This embodiment specifically includes the following steps: (1) Using peony seed meal, a by-product of peony seed oil extraction, as raw material, 100 kg of peony seed meal was crushed and passed through a 40-mesh sieve, then sprayed with water and stirred until it was moist. The amount of spraying water was 15 kg of the mass of the peony seed meal. The meal was then placed in a steaming and frying device and steamed and fried at 110 °C for 10 min. (2) Add 500 kg of water to the steamed and fried peony seed meal to prepare a suspension, adjust the pH value of the suspension to 8.0 ± 0.2 with protease, then add 1400 g of a complex enzyme consisting of cellulase, pectinase, α-amylase and β-amylase, mix well and perform enzymolysis at 55 °C for 2 hours; Among them, the complex enzymes include 467g of cellulase, 467g of pectinase, 233g of α-amylase, and 233g of β-amylase; (3) The enzymatic hydrolyzate was centrifuged at 6000 rpm for 6 min, the upper oil phase and the lower aqueous phase were collected separately, and the bottom filter residue was washed with water three times. The filtrate after washing was mixed with the lower aqueous phase to obtain a mixed aqueous phase; (4) The mixed aqueous phase was placed in a reactor and heated to 70°C. The mixed aqueous phase was then subjected to ultrasonic demulsification treatment at a high-low frequency combination of 24 kHz and 38 kHz, with an ultrasonic sound intensity of 1.8 W / cm and an ultrasonic time of 15 min. The demulsified mixed aqueous phase was allowed to stand, and the upper oil phase was separated and mixed with the oil phase obtained after enzymatic hydrolysis to obtain the extracted peony seed oil. (5) The peony seed oil is refined to obtain the final product.

[0023] The weight of the peony seed oil extracted in this example is 8.58 kg. The aqueous phase recovered in the final treatment system can be used as reclaimed water for other industries after detoxification, and the residue can be used for organic fertilizer or feed processing.

[0024] Example 3: A method for extracting peony seed oil from peony seed meal This embodiment specifically includes the following steps: (1) Using peony seed meal, a by-product of peony seed oil extraction, as raw material, 100 kg of peony seed meal was crushed and passed through a 40-mesh sieve, then sprayed with water and stirred until it was moist. The amount of spraying water was 12.5 kg of the peony seed meal mass. The meal was then placed in a steaming and frying device and steamed and fried at 105 °C for 12 min. (2) Add 450 kg of water to the steamed and fried peony seed meal to prepare a suspension, adjust the pH value of the suspension to 7.5 ± 0.2 with protease, then add 1100 g of a complex enzyme consisting of cellulase, pectinase, α-amylase and β-amylase, mix well and perform enzymolysis at 50 ° C for 2.5 hours; Among them, the complex enzymes include 367g of cellulase, 367g of pectinase, 183g of α-amylase, and 183g of β-amylase; (3) The enzymatic hydrolyzate was centrifuged at 5000 rpm for 8 min, the upper oil phase and the lower aqueous phase were collected separately, and the bottom filter residue was washed with water three times. The filtrate after washing was mixed with the lower aqueous phase to obtain a mixed aqueous phase; (4) The mixed aqueous phase was placed in a reactor and heated to 65°C, and then subjected to ultrasonic demulsification treatment at a high-low frequency combination of 22 kHz and 34 kHz, with an ultrasonic sound intensity of 1.5 W / cm and an ultrasonic time of 18 min. The demulsified mixed aqueous phase was allowed to stand, and the upper oil phase was separated and mixed with the oil phase obtained after enzymatic hydrolysis to obtain the extracted peony seed oil; (5) The peony seed oil is refined to obtain the final product.

[0025] The peony seed oil extracted in this example weighs 9.22 kg and is rich in α-linolenic acid, which can be used in the fields of health foods or cosmetics.

[0026] The refining process of peony seed oil in the above Examples 1-3 is a conventional process in this field, including filtration, hydration degumming, deacidification, decolorization, deodorization, dewaxing, etc., and various parameters and conditions are strictly controlled in this process to ensure that the product quality meets national standards and consumer demand. Therefore, it is no longer described in detail in the above examples.

[0027] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A method for extracting peony seed oil from peony seed meal, taking peony seed meal as the processing object, characterized in that: The method comprises the following steps: (1) crushing the peony seed meal, spraying water on it and stirring it until it is moist, and then placing it in a steaming and frying device, and steaming and frying it at 100-110°C; (2) adding water to the steamed and frying peony seed meal to mix it to form a suspension, adjusting the pH value of the suspension by using protease, and then adding a composite enzyme consisting of cellulase, pectinase, α-amylase and β-amylase for enzymatic hydrolysis; (3) centrifuging the enzymatic hydrolysis liquid, collecting the upper oil phase and the lower water phase respectively, and washing the bottom filter residue with water, mixing the washed filtrate with the lower water phase to obtain a mixed water phase; (4) placing the mixed water phase in a reactor and heating it to 60-70°C, and then ultrasonically demulsifying it, letting the demulsified mixed water phase stand, separating the upper oil phase, and mixing it with the oil phase obtained after enzymatic hydrolysis to obtain extracted peony seed oil; (5) refining the peony seed oil to finally obtain the product.

2. The method for extracting peony seed oil from peony seed meal as claimed in claim 1, wherein: In step (1), the peony seed meal is crushed and then passed through a 40-mesh sieve, the amount of spray water is 10-15% of the mass of the peony seed meal, and the meal is steamed and fried at 100-110° C. for 10-15 minutes.

3. The method for extracting peony seed oil from peony seed meal as claimed in claim 1, wherein: In step (2), the steamed and fried peony seed meal is mixed with water in a mass ratio of 1:4-5, the pH value of the suspension is adjusted to 7.0-8.0 by using protease, the amount of complex enzyme used is 0.8-1.4% of the mass of the peony seed meal, and enzymolysis is carried out at 45-55°C for 2-3 hours.

4. The method for extracting peony seed oil from peony seed meal as claimed in claim 1 or 3, wherein: In the complex enzyme, the mass ratio of cellulase, pectinase, α-amylase and β-amylase is 2:2:1:

1.

5. The method for extracting peony seed oil from peony seed meal as claimed in claim 1, wherein: In step (3), the enzymatic hydrolysate is centrifuged at 4000-6000 rpm for 6-10 min, and the bottom residue is washed with water 2-3 times.

6. The method for extracting peony seed oil from peony seed meal as claimed in claim 1, wherein: In step (4), the ultrasonic demulsification process of the mixed aqueous phase is carried out at a high-low frequency combination of 20-24 kHz and 30-38 kHz, with an ultrasonic sound intensity of 1.2-1.8 W / cm and an ultrasonic time of 15-20 min.

7. The method for extracting peony seed oil from peony seed meal as claimed in claim 6, wherein: The high-low frequency combinations for ultrasonic treatment of the mixed aqueous phase are: 20 KHz, 30 KHz; 22 KHz, 34 KHz or 24 KHz, 38 KHz.