Flexible and adaptable probe assembly, cell manipulation system, and probe reagent loading method

Abstract

This application discloses a flexible and adaptable probe assembly, a cell manipulation system, and a method for loading probe reagents. The flexible and adaptable probe assembly includes: a slender, hollow probe having a head and tail with opposing ends; a probe holder for positioning the probe, the probe holder having a loading channel connected to the tail; and a sheath, detachably connected to the probe holder and having a receiving cavity for accommodating the head. The technical solution disclosed in this application provides a stable control foundation for the probe through the integrated storage and use of the probe and probe holder, reducing the risk of probe damage, improving the operating experience, and providing a structural foundation for improving the probe usage environment.

Description

Technical Field

[0001] The present application relates to the field of cell manipulation, and in particular to a flexible and adaptable probe assembly, a cell manipulation system, and a probe reagent filling method. Background Art

[0002] When conducting cell research, it is necessary to observe the specific morphology of cells and extract the target cells separately for research. Cell manipulation is generally achieved through cell probes. In order to achieve micron and nanometer-level operation tips, cell probes are generally made of capillaries made of materials such as quartz and glass, which are drawn by laser heating. The front end of the cell probe is equipped with nanometer- or micrometer-level openings to achieve operations such as cell extraction, release, and intracellular injection.

[0003] In order to realize the storage and transportation of probes, the current technology generally confines the probes individually in independent packaging. When needed, they need to be taken out of the packaging and installed and connected in multiple steps. The operation is cumbersome and there is a risk of damage to the probes, so there is room for improvement. Summary of the Invention

[0004] In order to solve the above technical problems, the present application discloses a flexibly adaptable probe assembly, in which the probe can be flexibly adapted to the sheath and operating equipment through the probe seat, effectively avoiding the scenario in the prior art where direct operation of the probe is required, and reducing the risk of probe damage.

[0005] In one embodiment of the present application, a flexible and adaptable probe assembly is disclosed, comprising:

[0006] a probe having an elongated hollow structure and having opposing head and tail portions;

[0007] a probe seat for positioning the probe, the probe seat having a filling channel communicating with the tail;

[0008] and a sheath, which is detachably connected to the probe seat and has a receiving cavity for receiving the head.

[0009] Several optional methods are also provided below, but they are not intended to be additional limitations on the above-mentioned overall solution. They are merely further supplements or optimizations. Under the premise that there are no technical or logical contradictions, each optional method can be combined separately for the above-mentioned overall solution, or multiple optional methods can be combined.

[0010] In one embodiment, the probe assembly further includes an isolation frame disposed in the receiving cavity for preventing the sheath from contacting the probe during the separation process.

[0011] In one embodiment, one end of the isolation frame is a fixed end constrained by the probe seat, and the other end is a holding end for supporting the probe.

[0012] In one embodiment, the isolation frame provides an isolation cavity, and a side wall of the isolation frame is provided with an open window for allowing the probe and the probe seat to laterally enter and exit the isolation cavity.

[0013] In one embodiment, the fixed end of the isolation frame is provided with a through hole for the probe seat to pass through;

[0014] During the installation process, at least a portion of the probe seat extends from the isolation cavity through the through hole to the outside of the isolation cavity;

[0015] During the separation process, the probe seat retreats from the through hole to the isolation cavity and exits from the open window.

[0016] In one embodiment, the isolation cavity has a limiting portion for limiting the probe seat from retreating from the through hole to a limit position.

[0017] In one embodiment, the inner diameter of the isolation cavity on a side close to the probe seat is larger than the inner diameter of the isolation cavity on a side close to the head to provide the limiting portion.

[0018] In one embodiment, the isolation frame is provided with a retaining groove connected to the open window;

[0019] During the installation process, the middle portion of the probe enters and is accommodated in the retaining groove;

[0020] During the separation process, the middle portion of the probe exits the holding groove to achieve separation from the isolation frame.

[0021] In one embodiment, the retaining groove and the middle portion of the probe are clearance-matched.

[0022] In one embodiment, a gap is provided between the outer circumference of the spacer frame and the inner circumference of the sheath.

[0023] In one embodiment, the probe assembly further includes a base detachably connected to the probe seat, and the base is detachably connected to the sheath.

[0024] In one embodiment, the probe assembly further includes an isolation frame disposed in the receiving cavity, and the isolation frame is clamped and positioned by the probe seat and the base.

[0025] In one embodiment, the sheath is connected to the probe seat through the base, and the base is provided with a filling hole opening the filling channel.

[0026] In one embodiment, one end of the probe seat is a mounting section that cooperates with the operating device or the base, and the other end is a connecting section that cooperates with the probe. The mounting section is smaller in diameter than the connecting section and a positioning shoulder is provided between the two. The base and the positioning shoulder clamp each other to position the isolation frame.

[0027] In one embodiment, a gap is provided between the outer circumference of the connecting section and the inner circumference of the spacer.

[0028] One embodiment of the present application further discloses a cell operating system, comprising an operating device and the flexibly adaptable probe assembly described in the above technical solution;

[0029] The probe assembly has a use state and a storage state; in the storage state, the probe is positioned and connected to the sheath through the probe seat, and the head of the probe is suspended inside the receiving cavity; in the use state, the probe seat is separated from the sheath, and the probe is connected to the operating device through the probe seat;

[0030] The operating device includes a stage for carrying samples, a control component for moving the probe relative to the stage, a microscopic imaging component for providing a window for the probe, and a breathing component. The probe in the use state is connected to the control component through the probe seat. The control component positions the distal end of the probe to a preset position by controlling the clamp, and the breathing component drives the reagent into or out of the distal opening of the probe.

[0031] In one embodiment of the present application, a method for filling a probe reagent is also disclosed, comprising:

[0032] Providing a pipette and the probe assembly described in the above technical solution, wherein the pipette injects the reagent into the inner cavity of the probe through the filling channel;

[0033] The entire probe assembly is placed in a centrifugal device for centrifugal treatment, so that the reagent moves toward the head of the probe.

[0034] The technical solution disclosed in the present application provides a stable control basis for the probe through the overall storage and use of the probe and the probe holder, reduces the risk of probe damage, improves the operating experience, and provides a structural basis for improving the probe usage environment.

[0035] The specific beneficial technical effects will be further explained in conjunction with specific structures or steps in the specific implementation methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a schematic diagram of the structure of a probe assembly in one embodiment of the present application;

[0037] Figure 2 This is a schematic diagram of the structure of the probe assembly after the sheath is omitted in one embodiment of the present application;

[0038] Figure 3 for Figure 2 Schematic diagram of the coordination of the various parts of the probe assembly shown in ;

[0039] Figure 4 This is a schematic diagram of the probe assembly from the perspective of the base;

[0040] Figure 5 for Figure 4 Schematic diagram of the internal components of the probe assembly from the cross-sectional perspective at AA;

[0041] Figure 6 for Figure 5 Schematic diagram of the pipette and probe assembly under viewing angle;

[0042] Figure 7 Schematic diagram of a pipette loading reagents into the probe;

[0043] Figure 8 Schematic diagram of the distribution of the reagent in the probe after it moves in the axial direction of the probe under the action of centrifugation;

[0044] Figure 9 for Figure 4 Schematic diagram of the internal components of the probe assembly from the cross-sectional perspective at the middle BB;

[0045] Figure 10 and Figure 11 Schematic diagram of the probe, probe holder, and isolation rack at different stages of the separation process;

[0046] Figure 12 for Figure 11 Schematic diagram of the coordination of the probe, probe holder and isolation frame in a three-dimensional perspective;

[0047] Figure 13 This is a schematic diagram of the cell operating system framework in one embodiment of the present application;

[0048] Figure 14 Schematic diagram of the flow of the probe reagent filling method in one embodiment of the present application.

[0049] The reference numerals in the figures are described as follows:

[0050] 10. Stage; 21. Optical microscope objective lens; 22. Image detection unit; 23. Light source; 50. Moving platform; 60. Air pressure source assembly; 70. Control system;

[0051] 100, probe; 101, head; 102, tail; 110, probe seat; 111, loading channel; 112, positioning shoulder;

[0052] 300, sheath; 301, receiving cavity; 310, base; 311, loading hole; 312, cylinder; 313, disc; 320, isolation frame; 321, fixed end; 3211, through hole; 322, retaining end; 3221, retaining groove; 323, isolation cavity; 324, reduced diameter structure;

[0053] 901. Pipette. DETAILED DESCRIPTION

[0054] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0055] It should be noted that when a component is referred to as being "connected" to another component, it may be directly connected to the other component or there may be an intermediate component. When a component is referred to as being "disposed on" another component, it may be directly disposed on the other component or there may be an intermediate component.

[0056] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0057] In the prior art, before the probe is ready, the operator needs to carefully remove the probe from the package and position the probe on a specific holder. After calibrating the relative spatial position between the probe and the operating equipment, the corresponding cell operation can be performed. In the scenario where the probe needs to be filled with reagents, it is also necessary to position the probe on a specific fixture after removing the probe, fill the probe with reagents through a pipette, and then position the probe on a specific holder. The overall operation process is complicated and cumbersome, there is a risk of probe damage, it places high demands on the operator, and the operation efficiency is low.

[0058] In order to overcome the above technical problems, the present application discloses a flexibly adaptable probe assembly, comprising a probe 100, a probe base 110 connected to the probe 100, and a sheath 300 detachably connected to the probe base 110. The probe base 110 provides a stable control foundation for the probe 100, reducing the risk of damage to the probe 100, improving the operating experience, and allowing the probe 100 to be stably and quickly adapted to different scenarios, and providing a structural foundation for improvements in different usage environments.

[0059] For details, please refer to the attached Figure 1 To the attached Figure 3 The embodiment shown discloses a flexible and adaptable probe assembly. The probe 100 is a slender and hollow structured probe 100 having a head 101 and a tail 102 relative to each other. The probe base 110 positions the probe 100, and the probe base 110 has a loading channel 111 connected to the tail 102, and the loading channel 111 is connected to the interior of the probe 100. The sheath 300 is detachably connected to the probe base 110, and has a receiving cavity 301 for receiving the head 101. When the sheath 300 and the probe base 110 are connected to each other, the probe 100 is located inside the sheath 300. By exposing the loading channel 111 to the outside, the probe 100 can be operated when the sheath 300 is removed, thereby reducing the scenarios where the probe 100 needs to be exposed, facilitating operation and reducing the risk of damage.

[0060] Reference Attachment Figure 1 As shown, at this time, the probe assembly is in the storage state, the probe 100 is positioned and connected to the sheath 300 through the probe seat 110, and the head 101 of the probe 100 is suspended inside the receiving cavity 301; when the sheath 300 and the probe seat 110 are separated from each other, the probe 100 is exposed to the external environment from the sheath 300, refer to the attached Figure 11 And attached Figure 13 As shown, the probe assembly is in use at this time, the probe base 110 is separated from the sheath 300, and the probe 100 is connected to the operating device through the probe base 110.

[0061] The specific structure of the sheath 300 is combined with the attached Figure 3 As shown, the sheath 300 is cylindrical. Specifically, one end of the sheath 300 is open and the other end is closed. The sheath 300 extends from the head 101 of the probe 100 to the tail 102 of the probe 100 to protect the probe 100. The sheath 300 can directly cooperate with the probe base 110, or can cooperate with each other through other components, such as attached Figure 3 To the attached Figure 5As shown in , the probe assembly also includes a base 310 that is detachably connected to the probe seat 110, and the sheath 300 is connected to the probe seat 110 through the base 310. The base 310 is provided with a filling hole 311 with an open filling channel 111. In detail, the base 310 is provided with a first connecting structure radially inward and a second connecting structure radially outward, wherein the first connecting structure cooperates with the probe seat 110, and the second connecting structure cooperates with the sheath 300. The above-mentioned first connecting structure and second connecting structure can be realized by one or more mechanical positioning connection methods such as screw connection, clamping, and adsorption. The base 310 is an integral structure and is divided into a cylinder 312 for providing a connecting structure and a disk 313 located on one side of the cylinder 312, and the filling hole 311 runs through the cylinder 312 and the disk 313. The cylindrical body 312 cooperates with the outer edge of the probe base 110 to limit the maximum engagement position between the base 310 and the probe base 110. The disc 313 cooperates with the end surface of the sheath 300 to limit the maximum engagement position between the base 310 and the sheath 300. A drive unit is also provided on the outer circumference of the sheath 300 for driving the sheath 300 relative to the base 310. The drive unit moves the sheath 300 toward or away from the base 310, and accordingly, the probe 100 enters or exits the receiving cavity 301.

[0062] By determining the positional relationship between the probe base 110 and the sheath 300, the probe 100 can be stably suspended in the receiving cavity 301. In order to further improve the stability of the probe 100 to avoid the influence of external forces during storage and transportation and specific operations, the probe assembly also includes an isolation frame 320 disposed in the receiving cavity 301. The isolation frame 320 can prevent the sheath 300 from contacting the probe 100 during the separation process. Figure 3In the illustrated embodiment, one end of the isolation frame 320 is a fixed end 321 that is constrained by the probe base 110, and the other end is a retaining end 322 that supports the probe 100. The support can be implemented in a variety of specific ways, such as the retaining end 322 limiting the radial spatial position of the probe 100 away from the side of the tail 102, or the retaining end 322 cooperating with the outer circumference of the probe 100, etc. In the above-mentioned embodiments, the retaining end 322 and the outer circumference of the probe 100 are arranged in contact or with a gap. In the embodiment provided with the base 310, the isolation frame 320 is clamped and positioned by the probe base 110 and the base 310. In order to ensure the independence of the isolation frame 320 during the mating process of the various components, the isolation frame 320 is spatially positioned relative to the probe base 110 only through the fixed end 321. Specifically, the inner circumference of the isolation frame 320 is provided with a gap with the outer circumference of the probe base 110 (specifically, the connecting section of the probe base 110 mentioned below). Furthermore, a gap is set between the outer circumference of the isolation frame 320 and the inner circumference of the sheath 300. The above setting prevents the sheath 300 from affecting the isolation frame 320 and the probe 100 during movement, thereby further reducing the risk of damage. Specifically, the retaining end 322 acts on the middle part of the probe 100. In different embodiments, the retaining end 322 can be selected to act directly on the outer surface of the probe 100 to achieve positioning, or can be selected to be in stress-free contact with the probe 100 or be spaced apart and only curb the above trend when the probe 100 has a radial movement trend. In the attached Figure 9 To the attached Figure 12 In the illustrated embodiment, the isolation frame 320 is provided with a retaining groove 3221. The central portion of the probe 100 enters and is accommodated within the retaining groove 3221. The sidewalls of the retaining groove 3221 are arranged without stress contact or clearance with the outer circumference of the probe 100. The retaining groove 3221 is radially open to allow the probe 100 to enter or exit. Furthermore, the retaining groove 3221 is provided on the retaining end 322 and is connected to the open window.

[0063] In order to reduce the risk of damaging the probe 100 during the cooperation and separation process between the probe 100 and the isolation frame 320, refer to the attached Figure 9 To the attached Figure 12In the illustrated embodiment, during the switching process between the storage state and the use state of the probe assembly, the distal end of the probe 100 never exceeds the retaining end 322 in the extension direction of the probe 100. In specific details, the isolation frame 320 provides an isolation cavity 323, and the side wall of the isolation frame 320 is provided with an open window for the probe 100 and the probe base 110 to enter and exit the isolation cavity 323 laterally. The fixed end 321 of the isolation frame 320 is provided with a through hole 3211 for the probe base 110 to pass through. During the installation process, at least a portion of the probe base 110 (such as the installation section of the probe base 110 mentioned below) extends from the isolation cavity 323 through the through hole 3211 to the outside of the isolation cavity 323 and is connected to the base 310; during the separation process, the probe base 110 retreats from the through hole 3211 to the isolation cavity 323 and exits from the open window. Synchronously, the retaining groove 3221 is connected to the open window. During the installation process, the middle part of the probe 100 enters and is accommodated in the retaining groove 3221; during the separation process, the middle part of the probe 100 exits the retaining groove 3221 to achieve separation from the isolation frame 320. In the above process, the probe 100 and the probe seat 110 have a first direction movement in the axial direction of the probe 100 and a second direction movement in the radial direction of the probe 100. After the above actions are combined, the probe 100, the probe seat 110 and the isolation frame 320 can be coordinated and separated. In order to facilitate the probe 100 and the probe seat 110 to enter or exit the isolation frame 320, the fixed end 321 and the retaining end 322 are connected by multiple bar-shaped connecting rods to form a frame structure. The interior of the frame structure is an isolation cavity 323, and the gaps between the bar-shaped connecting rods are open windows. In order to limit the relative movement of the probe base 110 and the probe 100 relative to the isolation frame 320, the isolation cavity 323 has a limit portion that limits the probe base 110 to retreat from the through hole 3211 to the limit position. Figure 5 In the embodiment shown, the inner diameter of the isolation cavity 323 on the side close to the probe seat 110 is larger than the inner diameter of the isolation cavity 323 on the side close to the head 101, so as to provide a limit portion. That is, the inner diameter of the isolation cavity 323 decreases from the fixed end 321 to the retaining end 322, and the probe seat 110 cannot move to the retaining end 322. The above arrangement can be achieved by the mutual spacing of the bar-shaped connecting rods. Figure 12 As shown, in the extending direction of the probe 100 , the isolation frame 320 has a diameter-reducing structure 324 , and the diameter-reducing structure 324 provides a limiting portion by reducing the inner diameter of the isolation cavity 323 .

[0064] Independent of the above embodiments, the cooperation between the probe and the isolation frame in the present application can also be understood as disclosing a probe assembly that is easy to assemble, including:

[0065] A probe having an elongated and hollow structure, the probe having a head and a tail that are opposed to each other;

[0066] a probe holder for positioning the probe;

[0067] a sheath detachably connected to the probe base, and having a receiving cavity for receiving the head;

[0068] and an isolation frame located in the receiving cavity, the isolation frame providing an isolation cavity, and a side wall of the isolation frame being provided with an open window for the probe and the probe seat to enter and exit the isolation cavity laterally;

[0069] During installation, the probe holder and at least a portion of the probe enter the isolation cavity from the side through the open window and remain positioned;

[0070] During the separation process, the probe holder and the probe are released from their positions and laterally withdraw from the isolation cavity through the open window.

[0071] The isolation frame 320 can be connected to either or both of the probe holder 110 and the base 310 to achieve its own holding effect. Figure 3 In the illustrated embodiment, one end of the probe base 110 is a mounting section that mates with the operating device or base 310, and the other end is a connecting section that mates with the probe 100. The mounting section is smaller in diameter than the connecting section, and a positioning shoulder 112 is provided between the two. The base 310 and the positioning shoulder 112 mutually clamp the fixed end 321 of the positioning isolation frame 320. This arrangement simplifies the assembly relationship between the various components and improves the user experience. After separating the base 310 and the probe base 110, the probe base 110 and the isolation frame 320 can be automatically separated.

[0072] The focus of this application is on the overall storage and use of the probe 100 and the probe base 110, which provides a stable control basis for the probe 100. For example, one embodiment of this application also discloses a cell operating system, see the attached Figure 13 As shown, the cell operating system includes an operating device and the flexibly adaptable probe assembly in the above technical solution, and the probe assembly has the storage state and the use state mentioned above.

[0073] The operating device includes a stage 10 for carrying samples, a control component for moving the probe 100 relative to the stage, a microscopic imaging component for providing a window for the probe 100, and a breathing component. The probe 100 in use is connected to the control component through the probe seat 110. The control component positions the head 101 of the probe 100 to a preset position by controlling the probe seat 110. The breathing component drives the reagent into or out of the distal opening of the probe 100. The breathing component can specifically be a field source component that provides an electric field / magnetic field. The reagent enters or exits the probe 100 under the action of the electric field / magnetic field. The breathing component can also be attached to Figure 13 The air pressure source assembly 60 is shown in FIG. The air pressure source assembly 60 is connected to the probe 100 via the holder and the probe base 110, providing positive and negative air pressure to the probe 100 in the form of pulses. The air pressure source assembly 60 is used as a power source for aspirating and discharging substances (cells, reagents, etc.).

[0074] The microscopic imaging assembly includes a light source 23, an optical microscope objective lens 21, and an image detection unit 22 (e.g., a CCD camera). The light source is typically positioned directly above the stage 10, while the optical microscope objective lens 21 and image detection unit 22 are located below the stage. The control system 70, which can be a terminal device such as a computer, controls the air pressure source assembly 60, the motor in the control assembly's mobile platform 50, and the motor in the stage 10. The control system also includes an imaging display unit. This means that the probe 100 and target cells can be displayed on a screen through the imaging system, allowing the operator to perform real-time operations.

[0075] As mentioned above, the probe assembly in the present application also provides a structural basis for improving the use environment of the probe 100. For example, in a scenario where the probe 100 needs to be filled with reagents, the probe 100 is first removed from the package and then positioned to achieve filling, which is a cumbersome operation. The reagents in the present application refer to various liquid or gaseous media that can participate in cell operations, such as dyes, proteins, mRNA, plasmids and other common injection substances in the field of cell operations; for example, physiological saline, drugs, viruses, etc. that act on cells. The reagent can be a single substance or a combination of multiple substances. In one embodiment of the present application, a probe assembly that is easy to fill is disclosed, having a relative distal end (the side close to the probe seat 110 in the axial direction of the probe 100) and a proximal end (the side away from the probe seat 110 in the axial direction of the probe 100), refer to the attached. Figure 1 As shown, it includes a probe 100, a probe seat 110 and a sheath 300. The probe 100 is an elongated hollow structure, and the distal end of the inner cavity of the probe 100 is narrowed compared to the proximal end. The proximal end of the probe 100 is connected and positioned on the probe seat 110, and the probe seat 110 is provided with a through filling channel 111, and the filling channel 111 is connected to the inner cavity of the probe 100. The sheath 300 extends from the distal end of the probe 100 to the probe seat 110. The sheath 300 provides a closed cavity for accommodating the probe 100, and the filling channel 111 is connected to the outside of the closed cavity. In this embodiment, the positioning component in the probe 100 filling operation is realized by the sheath 300 itself, and the probe reagent can be filled without separating the probe 100 and the sheath 300, which effectively improves the filling efficiency and reduces the risk of damage to the probe 100.

[0076] During the reagent filling operation, the pipette 901 of the pipette gun is generally transported to the inside of the probe 100 to achieve the reagent filling. However, due to the extremely small internal size of the probe 100, the pipette 901 cannot completely fill the inside of the probe 100, especially the space at the head of the probe 100, which causes troubles in the subsequent cell operation process. In order to overcome the above problems, the attached Figure 14 One embodiment of the present application further discloses a method for filling a probe reagent, comprising:

[0077] A pipette and the probe assembly for easy filling as described in the above technical solution are provided, and the pipette injects the reagent into the inner cavity of the probe 100 through the filling channel 111;

[0078] The entire probe assembly is placed in a centrifugal device for centrifugal treatment, so that the reagent moves toward the head of the probe.

[0079] The centrifugal device and the probe assembly can be coordinated via a sheath 300, which is positioned within the centrifugal device to prevent direct exposure of the probe 100 to the centrifugal device. The centrifugal force of the centrifugal device drives the reagent to move axially within the probe 100. This axial movement of the reagent is specifically manifested as movement toward the head 101 (the region where the inner cavity is reduced in size) of the probe 100, thereby ensuring complete filling of the interior of the probe 100. In embodiments in which a spacer 320 is provided, the spacer 320 can assist in positioning the probe 100 (e.g., the support mentioned above) to resist the influence of the centrifugal force of the centrifugal device on the probe 100.

[0080] The above-mentioned probe reagent filling method drives the reagent in the probe 100 through a centrifugal device. The sheath 300 of the probe assembly provides a stable positioning basis for the centrifugation of the probe 100. The above-mentioned filling and centrifugation process can be carried out in the entire state where the probe 100, the probe seat 110 and the sheath 300 are not separated. Compared with the existing technology, it effectively optimizes the operation process and improves the user experience.

[0081] Furthermore, one embodiment of the present application also discloses a cell manipulation injection method, comprising:

[0082] Provide centrifugal equipment, operating equipment and the probe assembly mentioned above for easy loading;

[0083] The probe 100 and the probe seat 110 are positioned on the centrifugal device through the sheath 300. The reagent in the probe 100 moves to a preset position along the axial direction of the probe 100 under the driving force of the centrifugal device.

[0084] Separating the probe assembly from the centrifuge and separating the probe base 110 and the probe 100 from the sheath 300;

[0085] The probe 100 is mounted on a control assembly of an operating device via a probe holder 110 . The operating device controls the distal end of the probe 100 to be positioned at a preset position and drives the reagent to enter or exit through the distal end of the probe 100 .

[0086] In this embodiment, the probe 100, the probe holder 110, and the centrifugal device are adapted to each other through the sheath 300, and the probe 100 and the operating device are adapted to each other directly through the probe holder 110. Therefore, from another perspective, an embodiment of the present application also discloses a cell operating system, including a centrifugal device, an operating device, and the probe assembly mentioned above;

[0087] The centrifugal device includes a rotating platform and a positioning cavity provided on the rotating platform. The probe 100 is fitted into the positioning cavity through the sheath 300. The rotating platform is used to provide centrifugal force to drive the reagent in the probe 100 to move in the axial direction of the probe 100.

[0088] The operating device includes a stage for carrying samples, a control component for moving the probe 100 relative to the stage, a microscopic imaging component for providing a window for the probe 100, and a breathing component. The probe 100 separated from the sheath 300 is connected to the control component through the probe seat 110. The control component positions the distal end of the probe 100 to a preset position by controlling the probe seat 110, and the breathing component drives the reagent into or out of the distal opening of the probe 100.

[0089] The specific structures of the probe assembly and the operating device can be implemented in combination with the above description, and the specific structure of the centrifugal device can be implemented in combination with existing technologies, which will not be repeated here.

[0090] The technical features of the above-described embodiments may be combined in any manner. To simplify the description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as no contradiction exists between these combinations of technical features, they should be considered to be within the scope of this specification. When technical features of different embodiments are embodied in the same drawing, it can be deemed that the drawing also discloses examples of combinations of the various embodiments involved.

[0091] The above-described embodiments merely represent several implementation methods of the present application. While the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that a person skilled in the art may make various modifications and improvements without departing from the spirit of the present application, and such modifications and improvements are all within the scope of protection of the present application.

Claims

1. A flexible and adaptable probe assembly, characterized in that: include: a probe having an elongated hollow structure and having opposing head and tail portions; A probe seat for positioning the probe, wherein the probe is connected to a control assembly of an operating device via the probe seat, and the control assembly is used to move the probe relative to the stage; the probe seat has a filling channel connected to the tail portion, and a pipette injects the reagent into the inner cavity of the probe through the filling channel; a sheath, the sheath being detachably connected to the probe seat and having a receiving cavity for receiving the head, the sheath being capable of positioning the probe and the probe seat on a centrifugal device, so that the reagent in the probe moves axially along the probe to a preset position under the centrifugal force of the centrifugal device; An isolation frame is provided in the receiving cavity to prevent the sheath from contacting the probe during separation from the probe seat, one end of the isolation frame is a fixed end restricted by the probe seat, and the other end is a holding end for supporting the probe, and the fixed end of the isolation frame is provided with a through hole for the probe seat to pass through; the isolation frame provides an isolation cavity, and the side wall of the isolation frame is provided with an open window for the probe and the probe seat to enter and exit the isolation cavity laterally; During the installation process, at least a portion of the probe seat extends from the isolation cavity through the through hole to the outside of the isolation cavity; During the separation process, the probe seat retreats from the through hole to the isolation cavity and then exits from the open window.

2. The flexible adaptable probe assembly according to claim 1, characterized in that: The inner diameter of the isolation cavity decreases from the fixing end to the holding end, and the probe seat cannot move to the side of the holding end.

3. The flexible adaptable probe assembly according to claim 1, characterized in that: The isolation frame is provided with a retaining groove connected to the open window; During the installation process, the middle portion of the probe enters and is accommodated in the retaining groove; During the separation process, the middle portion of the probe exits the holding groove to achieve separation from the isolation frame.

4. The flexibly adaptable probe assembly according to claim 3, characterized in that: The retaining groove and the middle portion of the probe are clearance-matched.

5. The flexibly adaptable probe assembly according to claim 1, characterized in that: A gap is provided between the outer circumferential surface of the spacer frame and the inner circumferential surface of the sheath.

6. The flexibly adaptable probe assembly according to claim 1, characterized in that: The probe assembly further includes a base detachably connected to the probe seat, and the base is detachably connected to the sheath.

7. The flexibly adaptable probe assembly according to claim 6, characterized in that: The isolation frame is clamped and positioned by the probe seat and the base.

8. The flexibly adaptable probe assembly according to claim 7, characterized in that: One end of the probe seat is a mounting section that cooperates with the operating device or the base, and the other end is a connecting section that cooperates with the probe. The mounting section is smaller in diameter than the connecting section and a positioning shoulder is provided between the two. The base and the positioning shoulder clamp each other to position the isolation frame.

9. The flexibly adaptable probe assembly according to claim 8, characterized in that: A gap is provided between the outer circumferential surface of the connecting section and the inner circumferential surface of the isolation frame.

10. A cell operating system, characterized in that comprising an operating device and a flexibly adaptable probe assembly according to any one of claims 1 to 9; The probe assembly has a use state and a storage state; in the storage state, the probe is positioned and connected to the sheath through the probe seat, and the head of the probe is suspended inside the receiving cavity; in the use state, the probe seat is separated from the sheath, and the probe is connected to the operating device through the probe seat; The operating device includes a stage for carrying samples, a control component for moving the probe relative to the stage, a microscopic imaging component for providing a window for the probe, and a breathing component. The probe in the use state is connected to the control component through the probe seat. The control component positions the head of the probe to a preset position by controlling the probe seat, and the breathing component drives the reagent into or out of the opening at the head of the probe.

11. A method for filling a probe reagent, characterized in that: include providing a pipette and the probe assembly according to any one of claims 1 to 9, wherein the pipette injects a reagent into the inner cavity of the probe via the filling channel; The entire probe assembly is placed in a centrifugal device for centrifugal treatment, so that the reagent moves toward the head of the probe.

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