Preparation method of drought-resistant growth-promoting ammopiptanthus mongolicus charcoal coated seeds

Abstract

The invention discloses a preparation method of drought-resistant growth-promoting ammopiptanthus mongolicus charcoal coated seeds, and belongs to the field of seed processing, and the preparation method comprises the following steps: using 1.0% sodium carboxymethyl cellulose aqueous solution and 1.5% sodium carboxymethyl cellulose aqueous solution as binders, and coating the seeds in three layers by using a powder formula provided by the invention; according to the seed biochar coating technology, the emergence rate and the seedling fresh weight of the ammopiptanthus mongolicus seeds under drought stress can be effectively increased, growth of the ammopiptanthus mongolicus seeds in the seedling period is promoted, and the effects of full seedling and strong seedling are achieved. On the basis of obtaining the growth-promoting and seedling-strengthening effects, the size and weight of the seeds can be obviously increased, the smoothness and flowability of the seeds are effectively improved, and the method is suitable for large-scale mechanical precision seeding. In addition, the biochar is used as an active substance to carry out coating technology processing treatment on the ammopiptanthus mongolicus seeds, the method is novel, the cost is lower, operation is easy, and procedures are standardized.

Description

Technical Field

[0001] The invention relates to a method for preparing coated seeds, in particular to a method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds. Background Art

[0002] Ammopiptanthus mongolicus, a member of the genus Ammopiptanthus in the Leguminosae family, grows in sandy and gravelly deserts. It is a strong drought-tolerant plant, with leaves, stems, and roots exhibiting typical xeric structures that improve water utilization. As a drought-tolerant evergreen broad-leaved shrub in the semi-desert arid regions of northern my country, Ammopiptanthus mongolicus has nodules on its roots that improve soil quality. It has great potential for use in ecological restoration and as a horticultural ornamental plant, and has already been used as an ecological restoration species in some areas.

[0003] Drought is one of the most profound, widespread, and devastating climate disasters affecting global agricultural and forestry production. When plants are under drought conditions, water deficit not only affects their morphological structure but also leads to reduced leaf photosynthesis, reduced organic matter synthesis, metabolic disorders, and, in severe cases, plant death. Drought stress significantly increases the levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in plants. Simultaneously, to mitigate the damage caused by drought, the activity of protective enzymes such as peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) in plants is significantly enhanced. In the face of the severe global warming and increasing drought, studying the survival strategies of drought-tolerant forage grasses has become particularly important.

[0004] Seed coating is a time-honored traditional technique. It involves evenly coating seeds with a coating containing insecticides, fungicides, trace elements, and other additives without altering their properties. This technique boasts advantages such as ease of use, low cost, and high safety. This technique, formulated scientifically based on seed characteristics and the growing environment, plays a vital role in promoting seed germination and enhancing seed resistance, achieving the dual goals of increasing yield and commercial value.

[0005] Biochar is a high-carbon, stable organic material primarily produced by pyrolyzing biomass under anoxic conditions. Biochar has a large specific surface area, strong adsorption capacity, and good stability. It can improve soil properties, increase soil porosity, and enhance soil water retention capacity. In particular, it can increase the effective moisture content in the soil available for plant use, thereby increasing soil field water holding capacity. In recent years, based on biochar's strong adsorption, water retention, and organic nutritional properties, its use as an active substance for seed coating has been shown to not only promote seedling germination and growth, but also increase crop yield and nutritional value. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for preparing drought-resistant and growth-promoting mongolica biochar-coated seeds, so as to solve the problems of grassland forage grass emergence difficulties and low seedling survival rate and slow growth due to insufficient soil moisture content in the later stage.

[0007] To achieve the above objectives, the specific plan is as follows:

[0008] A method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds comprises the following steps:

[0009] S1: using 1.0% and 1.5% sodium carboxymethyl cellulose aqueous solutions as a binder, and coating the seeds in three layers using the powder formulation provided by the present invention;

[0010] Taking 10g of seeds as the adding unit of the powder formula, the powder formula includes:

[0011] (1) First layer: bentonite 5g + talc 5g; second layer: magnesium stearate 10g + bentonite 5g + talc 5g + biochar 3.3g; third layer: talc 4g;

[0012] (2) First layer: bentonite 5g + talc 5g; second layer: magnesium stearate 10g + bentonite 5g + talc 5g + biochar 2g; third layer: talc 4g;

[0013] S2: Sieve the washed and dried river sand using a 0.8 mm sieve, and then pour it into a transparent culture dish; evenly place the coated seeds in the river sand, then smooth the river sand, add distilled water to make the water content of the river sand reach 5%, and then place the transparent culture dish in a light incubator at a constant temperature of 25°C and light conditions for cultivation.

[0014] Furthermore, in step S1, the seed coating process steps are:

[0015] S1a: During the nucleation phase, 10 g of uniformly sized Ammopiptanthus mongolicus seeds were weighed and placed in a coating machine. The speed was set to 550 rpm to 650 rpm using the knob to ensure that the seeds could be lifted when rotating through the baffle. The atomizing disk was fixed to the outer wall to prevent slipping during the coating process. The switch was turned on. After the atomizing disk and the rolling tank rotated at a uniform speed, 0.2 to 0.5 mL of a 1% CMC aqueous solution was added to wet the seed surface. At this time, the height of the seeds lifted was reduced. Subsequently, a 1.5% CMC aqueous solution was continuously added to the atomizing disk, and the powder was continuously added at the same time. The above operation was repeated, and the powder and the binder were slowly and uniformly added alternately until the powder and the binder were added.

[0016] S1b: Increase the spheronization stage. After the powder is added during the film-forming stage, the speed is changed to 550-650 rpm. No powder is added and the seeds are idling for 2 minutes. Then the second portion of powder is added and the addition of 1.5% CMC aqueous solution is continued. The operation is cyclic, and the binder and powder are added in small amounts and multiple times until the powder and binder are added. Biochar is added at this stage.

[0017] S1c: Film-forming period: After the enlargement and spheronization period, keep the machine speed unchanged and idle the seeds for 2 to 3 minutes; add the powder and adhesive alternately, repeat the above operation, and stir for 10 seconds after a period of time; continue to rotate for 1 minute after adding the basic formula powder, and finally take out the Ammopiptanthus mongolicus coated seeds, pass them through a 24-14 mesh sieve, and screen the coated seeds of appropriate size; place the screened coated seeds of uniform size on absorbent paper to dry, and seal them in a polyethylene bag and store them at room temperature until further use.

[0018] Furthermore, 100 seeds were placed in each petri dish.

[0019] In summary, the present invention has the following beneficial effects compared to the prior art:

[0020] (1) The seed biochar coating technology of the present invention can effectively improve the germination rate and seedling fresh weight of Ammopiptanthus mongolicus seeds under drought stress, promote the growth of Ammopiptanthus mongolicus seeds during the seedling period, and achieve the effect of uniform and strong seedlings.

[0021] (2) The technology of the present invention can significantly increase the size and weight of seeds on the basis of achieving the above-mentioned effect of promoting growth and strengthening seedlings, effectively improve the smoothness and fluidity of seeds, and is suitable for large-scale mechanized precision sowing.

[0022] (3) The present invention uses biochar as an active substance to coat the Ammopiptanthus mongolicus seeds. The method is novel, has lower cost, is easy to operate, and has standardized procedures. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0024] Figure 1 The germination rate and seedling growth indexes of Ammopiptanthus mongolicus under different drought stress treatments;

[0025] Figure 2 To study the effects of biochar coating on seed emergence and seedling growth of Ammopiptanthus mongolicus under drought stress;

[0026] Figure 3 Effects of biochar coating on antioxidant enzyme activities in Ammopiptanthus mongolicus seedlings under drought stress;

[0027] Figure 4 These are the nCDA and RGB images of biochar-coated Ammopiptanthus mongolica seeds. DETAILED DESCRIPTION

[0028] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0029] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form can also include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0030] Unless otherwise specifically stated, the relative arrangement of the parts and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the drawings are not drawn according to actual proportional relationships. The techniques, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the techniques, methods and equipment should be considered as part of the authorized specification. In all examples shown and discussed herein, any specific values should be interpreted as being merely exemplary and not as limitations. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0031] The present invention provides a method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds, comprising the steps of:

[0032] S1: using sodium carboxymethyl cellulose aqueous solutions with concentrations of 1.0% and 1.5% as a binder, and coating seeds in three layers using the powder formulation provided by the present invention.

[0033] Taking 10g of seeds as the adding unit of the powder formula, the powder formula used in the present invention includes:

[0034] (1) First layer: bentonite (5 g) + talc (5 g); second layer: magnesium stearate (10 g) + bentonite (5 g) + talc (5 g) + biochar (3.3 g); third layer: talc (4 g).

[0035] (2) First layer: bentonite (5 g) + talc (5 g); second layer: magnesium stearate (10 g) + bentonite (5 g) + talc (5 g) + biochar (2 g); third layer: talc (4 g).

[0036] As a preferred embodiment, the seed coating process comprises the following steps:

[0037] S1a: During the nucleation phase, weigh 10g of uniformly sized Ammopiptanthus mongolicus seeds and place them in the coating machine. Set the speed to between 550 and 650 rpm using the knob to ensure the seeds are lifted to a certain height as they rotate through the baffle. Secure the atomizing disk to the outer wall to prevent slippage during the coating process. Turn on the switch. After the atomizing disk and roller can rotate at a constant speed, add 0.2-0.5mL of a 1% CMC aqueous solution to wet the seed surface, which will reduce the seed lift. Then, continuously add a 1.5% CMC aqueous solution to the atomizing disk while continuously adding the powder. Repeat this process, slowly and evenly adding the powder and binder alternately until the powder and binder are completely added.

[0038] S1b: Increase the spheronization stage. After the powder is added during the film-forming stage, change the speed to 550-650 rpm. Without adding any powder, let the seeds spin for about 2 minutes. Then add the second portion of powder, and continue adding the 1.5% CMC aqueous solution. Repeat this cycle, adding the binder and powder in small amounts until the powder and binder are all added. Biochar is added at this stage.

[0039] S1c: Film-forming stage: After the enlargement and spheronization phase, maintain the machine speed constant and idle the seeds for 2-3 minutes. Alternately add the powder and binder, repeating the process. Stir for 10 seconds after each interval. After adding the basic powder formula, continue rotating for about 1 minute. Finally, remove the coated Ammopiptanthus mongolicus seeds and screen them through a 24-14 mesh sieve to select the appropriate size. Place the appropriately sized coated seeds on absorbent paper to dry, then seal them in a polyethylene bag and store at room temperature until further use.

[0040] S2: Sieve the washed and dried river sand using a 0.8 mm sieve, and then pour it into a transparent culture dish; evenly place the coated seeds in it, then smooth the river sand, add distilled water to make the water content of the river sand reach 5%, and then place the transparent culture dish in a light incubator with a constant temperature of 25°C and light conditions for cultivation.

[0041] As a preference, 100 seeds are placed in each culture dish.

[0042] The experimental steps and results of the method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds provided by the present invention are as follows:

[0043] About seed coating technology:

[0044] 10 g of Ammopiptanthus mongolicus seeds were coated using a 5XW-110A seed coating machine and 1.0% and 1.5% sodium carboxymethylcellulose (CMC) aqueous solutions. The powder formulations are shown in the following table (Table 1).

[0045] Table 1 Coating powder formula

[0046]

[0047] The seed sand bed germination test was carried out in accordance with the grass seed inspection regulations germination test (GB / T2930.4-2017). The washed and dried river sand was sieved with a 0.8 mm sieve and placed in a 12×12×5 cm 3 Add 300g of river sand to a transparent Petri dish. Then, add 4%, 5%, or 7% (w / v = distilled water added mL / sand mass g) distilled water according to Table 2, with 10% water content as the control. Mix thoroughly. Place 100 bare seeds in each Petri dish, with four replicates per treatment. Cultivate in a light incubator maintained at a constant temperature of 25°C and under constant light conditions. Wipe away condensed water droplets on the Petri dish lids and walls every two days until seedlings emerge.

[0048] Table 2 Water content and distilled water consumption of each sand culture treatment

[0049]

[0050] Effect of drought stress conditions on the emergence rate of Ammopiptanthus mongolicus seeds (Example 1):

[0051] This experiment used commercially available Ammopiptanthus mongolicus seeds as the experimental material. 100 bare seeds were placed in each Petri dish on a sand bed with varying moisture contents, with four replicates per treatment. The seeds were cultured in a constant temperature and light incubator at 25°C. Condensed water droplets were wiped off the Petri dish lids and walls every two days until seedlings emerged. On the 14th day, the emergence rate was calculated, and 10 seedlings were randomly selected from the dishes for measurement of seedling length, root length, and fresh weight.

[0052] Seedling emergence rate (%) = number of seedlings emerged / number of test seeds × 100% (1)

[0053] The 10% water content was used as the control, and the sand bed with 4%, 5% and 7% water content was used to simulate drought stress. The results showed that the emergence rate of Ammopiptanthus mongolicus seeds decreased with the decrease of sand bed water content. Compared with the control group with 10% sand bed water content, the emergence rate was significantly reduced at 7% water content (P<0.05), and greatly reduced at 5% water content (P<0.05) ( Figure 1 A). As the water content of the sand bed decreased, the fresh weight of Ammopiptanthus mongolicus seedlings also showed a downward trend. When the water content was 7%, the fresh weight of the seedlings decreased significantly (P<0.05), and when the water content was 5%, the fresh weight of the seedlings decreased significantly (P<0.05) ( Figure 1 B). The change trend of seedling length is the same, decreasing with the decrease of sand bed moisture content. At 5% moisture content, the length of Ammopiptanthus mongolicus seedlings has been significantly reduced (P<0.05) ( Figure 1 C). The root length of Ammopiptanthus mongolicus seedlings also gradually decreased with the decrease of sand bed water content, and decreased significantly at 5% water content (P<0.05) ( Figure 1 D) Based on the above results, a sand bed moisture content of 5% was used as the drought stress condition for subsequent experiments on Ammopiptanthus mongolicus seeds.

[0054] Effect of biochar coating on seed germination of Ammopiptanthus mongolicus under drought stress (Example 2):

[0055] 5% sand bed moisture content was selected as the drought stress condition, and bare seeds and basic coated seeds were used as controls. The Ammopiptanthus mongolicus seeds were coated with four biochar coating formulas, namely BC1-15, BC1-10, BC1-5 and BC1-3.

[0056] The germination test of the coated seeds showed that under drought stress, all biochar coating treatments significantly (P<0.05) increased the germination rate of Ammopiptanthus mongolicus seeds compared with CK1. Compared with CK1 and CK2, BC1-3 treatment significantly (P<0.05) increased the germination rate by 37.7% compared with CK1 and 23.7% compared with CK2. Figure 2A). Compared with CK1, all coating treatments significantly (P<0.05) increased the fresh weight of Ammopiptanthus mongolicus seedlings. Compared with CK1 and CK2, BC1-5 and BC1-3 biochar coating treatments significantly (P<0.05) increased the fresh weight of Ammopiptanthus mongolicus seedlings. BC1-3 had the best effect, increasing the fresh weight by 316% and 38.8% compared with CK1 and CK2, respectively. Figure 2 B). Biochar coating did not significantly promote the growth of Ammopiptanthus mongolicus seedlings and roots ( Figure 2 C, D). Based on the above results, BC1-3 biochar coating treatment is the best for improving the drought tolerance of Ammopiptanthus mongolicus.

[0057] CK1 represents bare seeds; CK2 represents basic coated seeds (no biochar added); BC1-15 represents 0.7 g of biochar added per 10 g of bare seeds; BC1-10 represents 1.0 g of biochar added per 10 g of bare seeds; BC1-5 represents 2.0 g of biochar added per 10 g of bare seeds; and BC1-3 represents 3.3 g of biochar added per 10 g of bare seeds. Different lowercase letters indicate significant differences at the 0.05 level.

[0058] Effects of biochar coating on physiological indicators of Ammopiptanthus mongolicus seedlings under drought stress (Example 3):

[0059] To further explore the mechanism of biochar coating on seedling tolerance to drought stress, various physiological indicators of Ammopiptanthus mongolicus seedlings treated with BC1-3 biochar were measured. The results showed that compared with CK1 and CK2, the activities of six antioxidant enzymes in Ammopiptanthus mongolicus seedlings treated with BC1-3 biochar were increased ( Figure 3 AF). Compared with CK1, BC1-3 biochar coating treatment significantly (P<0.05) increased GR activity in Ammopiptanthus mongolicus seedlings by 61% ( Figure 3 B). Compared with CK1 and CK2, BC1-3 biochar coating treatments significantly (P<0.05) increased the MDHAR, POD, SOD and APX activities of Ammopiptanthus mongolicus seedlings by 733%, 129%, 104% and 47% respectively compared with CK1, and by 56%, 50%, 93% and 25% respectively compared with CK2 ( Figure 3 The above results indicate that BC1-3 biochar coating treatment can enhance the tolerance of Ammopiptanthus mongolicus seedlings to drought stress by increasing the activity of antioxidant enzymes.

[0060] In the figures above, the ABC notation is used to indicate significant differences between groups of data. The significance of the differences between groups is indicated by arranging the means from highest to lowest and labeling them with letters (e.g., a, b, c, etc.). Identical letters indicate an insignificant difference, while different letters indicate a significant difference.

[0061] The steps for marking abcd are:

[0062] (1) First, arrange all the averages in order from largest to smallest, and then mark the largest average with the letter a.

[0063] (2) Compare this mean with the following means. Any mean with insignificant difference is marked with the letter a, until a mean with significant difference is found, which is marked with the letter b.

[0064] (3) Taking the largest mean number marked with b as the standard, compare it with the following unmarked means. Any insignificant ones will continue to be marked with the letter b until a mean number marked with c is found that is significantly different from it.

[0065] (4) The differences between the two groups were not significant if they had the same letter, and the differences between the two groups were significant if they had different letters. Lowercase letters indicate a significance level of a = 0.05.

[0066] Performance evaluation of coated seeds using multispectral technology (Example 4):

[0067] Multispectral imaging is an advanced imaging technology that uses spectral information from multiple wavelengths to capture and analyze the characteristics of an object. Unlike traditional visible light imaging, multispectral imaging can acquire image data across the visible, near-infrared, and other spectral ranges. By using specialized sensors and cameras, this technology can simultaneously record different wavelengths of light, providing richer information.

[0068] Images of biochar- and iron-fertilizer-coated seeds were acquired using a multispectral imaging instrument, VideometerLab 4 (Videometer A / S, Herlev, Denmark). Prior to acquisition, the light source must be calibrated. The system was fully radiometrically and geometrically calibrated using three different plates: a white plate, a black plate, and a white dot plate. After calibration, the seed sample was placed at the bottom of the instrument's sphere and illuminated using high-power light-emitting diodes (LEDs) located at the edge of the sphere. Within seconds, a high-resolution, 2056 × 2056-pixel multispectral raw image was acquired. This raw image covers 19 wavelengths: 365, 405, 430, 450, 470, 490, 515, 540, 570, 590, 630, 645, 660, 690, 780, 850, 880, 940, and 970 nm.

[0069] Normalized Canonical Discriminant Analysis (nCDA) is a method used to distinguish seeds from background. It can divide seeds into regions of interest with different spectral characteristics. Based on the nCDA model in VideometerLab 4 software, seed models were constructed for different biochar and iron fertilizer coating treatments. Due to the differences in average spectral reflectance between different treatments, the reflectance difference between high-vitality seeds and low-vitality seeds is significant. Therefore, in the multispectral image after background removal, the seeds are divided into two layers: seeds with higher vitality appear blue, while seeds with lower vitality appear red.

[0070] Multispectral analysis of biochar-coated Ammopiptanthus mongolicus seeds, bare seeds, and basic coated seeds showed that ( Figure 4 ), in the nCDA image, the color of Ammopiptanthus mongolicus seeds treated with biochar was bluer compared with CK1 and CK2, among which the color of Ammopiptanthus mongolicus seeds treated with BC1-3 biochar was the bluest and had the best effect.

[0071] Cost Analysis (Example 5):

[0072] Based on these results, a cost analysis was conducted for the most effective coating formulation. Industrial prices for the coating powder formulation materials were obtained from relevant platforms (Table 3). The market price of bare Ammopiptanthus mongolicus seeds is approximately 125 yuan / kg. The dosage and cost of biochar-coated seeds were calculated for the same seedling emergence rate. The results (Table 4) show that sowing costs per hectare of Ammopiptanthus mongolicus seeds treated with BC1-3 biochar were 4204 to 5110 yuan lower than sowing bare seeds.

[0073] Table 3 Prices of biochar and coating materials

[0074]

[0075] Note: The dosage is the amount required to process 1kg of bare seeds. Price data is based on the combined prices of multiple merchants on the Alibaba platform. The prices here do not represent actual prices.

[0076] Table 4. Sowing costs of bare, basic-coated, and biochar-coated Ammopiptanthus mongolicus seeds

[0077]

[0078] Note: CK1 represents bare seeds; CK2 represents basic coated seeds; BC1-3 represent the ratio of biochar to bare seeds of 1:3. The sowing rate of coated seeds is calculated based on the same number of seedlings per hectare as bare seeds.

[0079] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds, characterized in that: Including steps: S1: using 1.0% and 1.5% sodium carboxymethyl cellulose aqueous solutions as a binder, and coating the seeds in three layers using the powder formulation provided by the present invention; Taking 10g of seeds as the adding unit of the powder formula, the powder formula includes: (1) First layer: bentonite 5g + talc 5g; second layer: magnesium stearate 10g + bentonite 5g + talc 5g + biochar 3.3g; third layer: talc 4g; (2) First layer: bentonite 5g + talc 5g; second layer: magnesium stearate 10g + bentonite 5g + talc 5g + biochar 2g; third layer: talc 4g; S2: Sieve the washed and dried river sand using a 0.8 mm sieve, and then pour it into a transparent culture dish; evenly place the coated seeds in the river sand, then smooth the river sand, add distilled water to make the water content of the river sand reach 5%, and then place the transparent culture dish in a light incubator at a constant temperature of 25°C and light conditions for cultivation.

2. The method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds according to claim 1, characterized in that: In step S1, the seed coating process steps are: S1a: During the nucleation phase, 10 g of uniformly sized Ammopiptanthus mongolicus seeds were weighed and placed in a coating machine. The speed was set to 550 rpm to 650 rpm using the knob to ensure that the seeds could be lifted when rotating through the baffle. The atomizing disk was fixed to the outer wall to prevent slipping during the coating process. The switch was turned on. After the atomizing disk and the rolling tank rotated at a uniform speed, 0.2 to 0.5 mL of a 1% CMC aqueous solution was added to wet the seed surface. At this time, the height of the seeds lifted was reduced. Subsequently, a 1.5% CMC aqueous solution was continuously added to the atomizing disk, and powder was continuously added at the same time. The above operation was repeated, and the powder and binder were slowly and uniformly added alternately until the powder and binder were added. S1b: Increase the spheronization stage. After the powder is added during the film-forming stage, the speed is changed to 550-650 rpm. No powder is added and the seeds are idling for 2 minutes. Then the second portion of powder is added and the addition of 1.5% CMC aqueous solution is continued. The operation is cyclic, and the binder and powder are added in small amounts and multiple times until the powder and binder are added. Biochar is added at this stage. S1c: Film formation stage: after the enlargement and spheronization stage, keep the machine speed unchanged and let the seeds idle for 2-3 minutes; add the powder and binder alternately, repeat the above operation, and stir for 10 seconds after a period of time; After adding the basic formula powder, continue to rotate for 1 minute, finally take out the coated seeds of Ammopiptanthus mongolicus and sieve them; place the coated seeds of uniform size screened out on absorbent paper to dry, seal them in a polyethylene bag and store them at room temperature until further use.

3. The method for preparing drought-resistant and growth-promoting Ammopiptanthus mongolicus biochar-coated seeds according to claim 1, characterized in that: 100 seeds were placed in each Petri dish.

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