South Yunnan Chinese bee double-queen cultivation technology method

Abstract

The invention relates to the technical field of double-queen cultivation of southern Yunnan Chinese bees, in particular to a method of a double-queen cultivation technology of southern Yunnan Chinese bees, which comprises the following steps: preferably selecting a local high-yield disease-resistant bee species, intervening a same-period queen cell into a standardized bee colony, constructing a double-area structure by using a vertical partition plate, applying honey liquid to the same-age queen bees, and then intervening in different areas. The size of the hive entrance is dynamically adjusted according to the group potential difference, worker bee channels are opened in stages, and the partition plates are removed to combine the double-area swarms. The elsholtzia rugulosa plant extract is mixed in a gradient manner to replace alcohol, so that the rejection behavior of worker bees is avoided; group potential balance is controlled in a linkage mode through dynamic hive entrance regulation and control and cover sealing spleen threshold value exchange; the staged partition plate channels are opened, the worker-bee interaction rate larger than or equal to 70% serves as a switching node, and two-area pheromone progressive fusion is achieved; through combination of overwintering bee group temperature closed-loop control and queen bee spacing precise management, the double-queen coexistence stability is finally improved to 90% or above, and the upper limit of the bee group potential in southern Yunnan is broken through to the scale of 12 honeybees.

Description

Technical Field

[0001] The invention relates to the technical field of breeding double queens of bees in southern Yunnan, and in particular to a method for breeding double queens of bees in southern Yunnan. Background Art

[0002] The Yunnan Chinese honeybee is a local population of the Chinese honeybee (Apis ceranaindica) distributed in the subtropical climate zone of the low-latitude plateau in southern Yunnan. It has unique ecological adaptability. The birth weight of its queen and the number of egg tubules are significantly affected by seasonal nutritional conditions. It can maintain a stable egg-laying capacity in a mountainous environment with an average annual temperature of 18.6°C and high humidity. However, under the traditional single-queen breeding model, the colony strength is relatively weak and it is usually difficult to break through the scale limit of 6-7 honeycombs.

[0003] Since the natural colonies of Chinese honeybees in southern Yunnan are relatively small and the queen bee's daily egg-laying capacity is limited, single-queen colonies reproduce slowly and it is difficult to form strong colonies before the nectar flow period to support high-yield collection. However, double-queen colonies can significantly accelerate reproduction by doubling the egg-laying capacity, increasing the number of overwintering bees by more than 40%, and utilizing the mutual warming characteristics of the colonies in the two areas to enhance the overwintering survival rate, thereby solving the bottleneck problem of small-scale and low-yield Chinese honeybee farming in mountainous areas. Therefore, double-queen breeding is crucial.

[0004] However, in general, traditional methods rely on wooden partitions to separate beehives, and introduce double queens after briefly unifying the colony's smell by spraying white wine or alcohol. However, gaps in the partitions can easily lead to queens visiting and fighting each other, and the distance between the hive doors is less than 20 cm, which causes worker bees to gather lopsidedly. When nectar sources are scarce, worker bees are likely to eliminate weak queens. At the same time, the separation of the two bee colonies during the wintering period can easily lead to the loss of the old queen. In addition, improper double-bee transfer operations can easily lead to uneven queen quality, ultimately resulting in a double-queen colony maintenance success rate of less than 30%.

[0005] Based on this, the present invention provides a method for breeding double queens of honeybees in southern Yunnan to solve the above-mentioned technical problems. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for breeding double queens of honeybees in southern Yunnan, so as to solve the problems mentioned in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] The present invention provides a method for breeding double queen bees in southern Yunnan, comprising the following steps:

[0009] S1. Select local high-yield and disease-resistant bee species and cultivate high-quality queen bees through enhanced nutrition and multiple bee transplanting operations;

[0010] S2. Insert the queen cells of the same period into the standardized bee colony and spray the colony with a special plant liquid to unify the colony's flavor;

[0011] S3. Using vertical partitions to create a dual-zone structure, the plant extracts are simultaneously applied to blend the scents of the two zones.

[0012] S4. After queen bees of the same age apply honey, they intervene in different areas and monitor worker bee behavior in real time to prevent conflicts.

[0013] S5. Dynamically adjust the size of the nest entrance according to the difference in colony strength, and exchange brood combs to balance the bee population;

[0014] S6. Periodically optimize the layout of the brood spleen and supplement with special feed to maintain balanced feeding between the two areas;

[0015] S7. Open worker bee channels in stages, using group interaction rates as nodes to promote swarm integration;

[0016] S8. Remove the partitions and merge the two-zone bee colonies, optimize the honeycomb layout and insulation to promote the fusion of bee colonies.

[0017] Preferably, the implementation steps of step S1 are:

[0018] Select queen bees with stable egg-laying ability, an average daily egg-laying capacity of no less than 1,100 eggs, and strong disease resistance from high-quality local bee colonies in southern Yunnan as mother bees, and cultivate queen cells through artificial transfer;

[0019] The queen bee is introduced into the strong colony 45 days in advance to strengthen the feeding, and is fed 100 ml of pollen paste mixed with pollen and honey at a mass ratio of 1 to 1.5 every day to ensure that the larvae are fully nourished;

[0020] When cultivating queen cells, select larvae within 12 hours of hatching and transfer them into the queen cell cups, and complete the double transfer operation within 24 hours after transfer to improve the quality of the queen.

[0021] Preferably, the implementation steps of step S2 are:

[0022] The queen cells cultivated in step S1 are placed in the pre-selected mating group on the 6th day after capping;

[0023] The mating group is equipped with 3 honeycombs per group, including 1 capped honeycomb with a cap ratio of 60%, 1 honey and pollen honeycomb and 1 egg and larvae honeycomb, maintaining a honeycomb ratio of 1.2 to 1;

[0024] Two hours before intervention, spray 5% concentration of Yebazi plant extract evenly into the colony to unify the colony smell and avoid worker bee rejection behavior.

[0025] Preferably, the implementation steps of step S3 are:

[0026] Use a modified twelve-frame beehive, with the interior of the hive divided into two zones by vertical queen excluders with a grid spacing of 3.8 mm plus or minus 0.1 mm. The hive doors are located on the front and back sides of the hive, with a spacing of no less than 35 cm.

[0027] On the third day after successful mating in step S2, evenly spray the surfaces of the honeycombs in both areas with 10 ml of Yebazi extract per honeycomb to completely cover the original colony odor;

[0028] At the same time, through real-time monitoring by temperature and humidity sensors in the box, the temperature of the breeding area is strictly controlled at 34.5 degrees Celsius plus or minus 0.5 degrees Celsius, and the humidity is maintained at 65% plus or minus 5%.

[0029] Preferably, the implementation steps of step S4 are:

[0030] Select two queen bees of the same age that have successfully laid eggs in step S2 and whose age difference is no more than 24 hours, and simultaneously introduce them into the two zones in the evening;

[0031] During the operation, apply wild honey from southern Yunnan diluted to 30% concentration on the queen bee's chest, and then gently place it in the lower middle part of the honeycomb in the two zones;

[0032] An infrared monitoring system was used to continuously monitor the frequency of antennae contact among worker bees within 48 hours after intervention. If the attack behavior exceeded 5 times per minute, Yebazi extract was sprayed immediately.

[0033] Preferably, the implementation steps of step S5 are:

[0034] Dynamically adjust the nest gate size based on the difference in bee attendance between the two zones recorded on the seventh day after the intervention in step S4. When the difference in bee attendance exceeds the 15% threshold, limit the width of the nest gate to 1.5 cm for strong colonies and expand it to 2.5 cm for weak colonies.

[0035] The brood comb replacement operation is performed simultaneously: a brood comb with a capped hatch rate of not less than 80% is transferred from the strong area to the weak area, and at the same time, a brood comb with an egg density of more than 90% is transferred from the weak area to the strong area.

[0036] Preferably, the implementation steps of step S6 are:

[0037] Adjust the layout of the brood spleen every 5 days, keeping the proportion of capped spleens at 40% to 45%, the proportion of oviparous spleens at 30% to 35%, and reserving 20% of empty spleens for egg laying;

[0038] According to the group strength data recorded in step S5, 50 grams of pollen cakes mixed with pollen, soybean powder and honey in a mass ratio of 6:3:1 are supplemented to each area every day, and 200 ml of syrup with a sugar-water ratio of 1:1 is fed simultaneously.

[0039] Preferably, the implementation steps of step S7 are:

[0040] On the 21st day after the intervention of step S4, when egg laying stabilized, partition regulation was initiated: in the first stage, 30% of the worker bee channel area was opened, and the interaction rate of the worker bees in the two areas was tested after 3 days; when the comprehensive interaction rate of antennal contact and food transfer reached more than 70%, the second stage was entered, and the channel was opened to 60% for 4 days; after reaching the interaction rate threshold again, the third stage was entered, and the channel was fully opened while retaining the physical separation.

[0041] Preferably, the implementation steps of step S8 are:

[0042] 15 days before the Beginning of Winter, when the outside temperature drops below 10 degrees Celsius, remove the queen excluder and merge the two-zone bee colonies;

[0043] According to the brood comb data in step S6, two capped brood combs and one honey and pollen comb are reserved and placed in the center of the box, and the distance between the two queen bees is controlled to be within the range of 8 to 10 cm;

[0044] The outside of the box is wrapped with a 3 cm thick straw insulation layer, the nest entrance is reduced to 1.0 cm, and the temperature inside the box is continuously maintained in the range of 20 degrees Celsius plus or minus 2 degrees Celsius.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] The present invention replaces alcohol with gradient flavoring of wild bazi plant extract to avoid the rejection behavior of worker bees; dynamic nest door regulation and the capped honeycomb replacement threshold are linked to control the balance of colony strength; the partition channel is opened in stages with the worker bee interaction rate ≥ 70% as the switching node to achieve the gradual fusion of pheromones in the two areas; combined with the closed-loop temperature control of the overwintering bee colony and the precise management of the distance between queens, the stability of the coexistence of two queens is finally improved to more than 90%, and the upper limit of the colony strength of the Chinese honeybee in southern Yunnan is broken to a scale of 12 honeycombs. DETAILED DESCRIPTION

[0047] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0048] Example 1, the present invention proposes a method for breeding double queen bees in southern Yunnan, comprising the following steps:

[0049] S1. Select local high-yield and disease-resistant bee species and cultivate high-quality queen bees through enhanced nutrition and multiple bee transplanting operations;

[0050] S2. Insert the queen cells of the same period into the standardized bee colony and spray the colony with a special plant liquid to unify the colony's flavor;

[0051] S3. Using vertical partitions to create a dual-zone structure, the plant extracts are simultaneously applied to blend the scents of the two zones.

[0052] S4. After queen bees of the same age apply honey, they intervene in different areas and monitor worker bee behavior in real time to prevent conflicts.

[0053] S5. Dynamically adjust the size of the nest entrance according to the difference in colony strength, and exchange brood combs to balance the bee population;

[0054] S6. Periodically optimize the layout of the brood spleen and supplement with special feed to maintain balanced feeding between the two areas;

[0055] S7. Open worker bee channels in stages, using group interaction rates as nodes to promote swarm integration;

[0056] S8. Remove the partitions and merge the two-zone bee colonies, optimize the honeycomb layout and insulation to promote the fusion of bee colonies.

[0057] Furthermore, the implementation steps of step S1 are:

[0058] Select queen bees with stable egg-laying ability, an average daily egg-laying capacity of no less than 1,100 eggs, and strong disease resistance from high-quality local bee colonies in southern Yunnan as mother bees, and cultivate queen cells through artificial transfer;

[0059] The queen bee is introduced into the strong colony 45 days in advance for intensive feeding, and is fed 100 ml of pollen paste mixed with honey at a mass ratio of 1 to 1.5 every day to ensure that the larvae are fully nourished.

[0060] When cultivating queen cells, select larvae within 12 hours of hatching and transfer them into the queen cell cups, and complete the double transfer operation within 24 hours after transfer to improve the quality of the queen.

[0061] Furthermore, the implementation steps of step S2 are:

[0062] The queen cells cultivated in step S1 are placed in the pre-selected mating group on the 6th day after capping;

[0063] The mating group is equipped with 3 honeycombs per group, including 1 capped honeycomb with a cap ratio of 60%, 1 honey and pollen honeycomb and 1 egg and larvae honeycomb, maintaining a honeycomb ratio of 1.2 to 1;

[0064] Two hours before intervention, spray 5% concentration of Yebazi plant extract evenly into the colony to unify the colony smell and avoid worker bee rejection behavior.

[0065] Furthermore, the implementation steps of step S3 are:

[0066] Use a modified twelve-frame beehive, with the interior of the hive divided into two zones by vertical queen excluders with a grid spacing of 3.8 mm plus or minus 0.1 mm. The hive doors are located on the front and back sides of the hive, with a spacing of no less than 35 cm.

[0067] On the third day after successful mating in step S2, evenly spray the surfaces of the honeycombs in both areas with 10 ml of Yebazi extract per honeycomb to completely cover the original colony odor;

[0068] At the same time, through real-time monitoring by temperature and humidity sensors in the box, the temperature of the breeding area is strictly controlled at 34.5 degrees Celsius plus or minus 0.5 degrees Celsius, and the humidity is maintained at 65% plus or minus 5%.

[0069] Furthermore, the implementation steps of step S4 are:

[0070] Select two queen bees of the same age that have successfully laid eggs in step S2 and whose age difference is no more than 24 hours, and simultaneously introduce them into the two zones in the evening;

[0071] During the operation, apply wild honey from southern Yunnan diluted to 30% concentration on the queen bee's chest, and then gently place it in the lower middle part of the honeycomb in the two zones;

[0072] An infrared monitoring system was used to continuously monitor the frequency of antennae contact among worker bees within 48 hours after intervention. If the attack behavior exceeded 5 times per minute, Yebazi extract was sprayed immediately.

[0073] Furthermore, the implementation steps of step S5 are:

[0074] Dynamically adjust the nest gate size based on the difference in bee attendance between the two zones recorded on the seventh day after the intervention in step S4. When the difference in bee attendance exceeds the 15% threshold, limit the width of the nest gate to 1.5 cm for strong colonies and expand it to 2.5 cm for weak colonies.

[0075] The brood comb replacement operation is performed simultaneously: a brood comb with a capped hatch rate of not less than 80% is transferred from the strong area to the weak area, and at the same time, a brood comb with an egg density of more than 90% is transferred from the weak area to the strong area.

[0076] Furthermore, the implementation steps of step S6 are:

[0077] Adjust the layout of the brood spleen every 5 days, keeping the proportion of capped spleens at 40% to 45%, the proportion of oviparous spleens at 30% to 35%, and reserving 20% of empty spleens for egg laying;

[0078] According to the group strength data recorded in step S5, 50 grams of pollen cakes mixed with pollen, soybean powder and honey in a mass ratio of 6:3:1 are supplemented to each area every day, and 200 ml of syrup with a sugar-water ratio of 1:1 is fed simultaneously.

[0079] Furthermore, the implementation steps of step S7 are:

[0080] On the 21st day after the intervention of step S4, when egg laying stabilized, partition regulation was initiated: in the first stage, 30% of the worker bee channel area was opened, and the interaction rate of the worker bees in the two areas was tested after 3 days; when the comprehensive interaction rate of antennal contact and food transfer reached more than 70%, the second stage was entered, and the channel was opened to 60% for 4 days; after reaching the interaction rate threshold again, the third stage was entered, and the channel was fully opened while retaining the physical separation.

[0081] Furthermore, the implementation steps of step S8 are:

[0082] 15 days before the Beginning of Winter, when the outside temperature drops below 10 degrees Celsius, remove the queen excluder and merge the two-zone bee colonies;

[0083] According to the brood comb data in step S6, two capped brood combs and one honey and pollen comb are reserved and placed in the center of the box, and the distance between the two queen bees is controlled to be within the range of 8 to 10 cm;

[0084] The outside of the box is wrapped with a 3 cm thick straw insulation layer, the nest entrance is reduced to 1.0 cm, and the temperature inside the box is continuously maintained in the range of 20 degrees Celsius plus or minus 2 degrees Celsius.

[0085] Example 2: In practical application, the present invention proposes a method for breeding double queen bees in southern Yunnan, which specifically includes the following steps:

[0086] S1. Select local high-yield and disease-resistant bee species and cultivate high-quality queen bees through enhanced nutrition and multiple bee transplanting operations;

[0087] In this embodiment, it should be noted that the implementation steps of step S1 are:

[0088] Select queen bees with stable egg-laying ability, an average daily egg-laying capacity of no less than 1,100 eggs, and strong disease resistance from high-quality local bee colonies in southern Yunnan as mother bees, and cultivate queen cells through artificial transfer;

[0089] The queen bee is introduced into the strong colony 45 days in advance to strengthen the feeding, and is fed 100 ml of pollen paste mixed with pollen and honey at a mass ratio of 1 to 1.5 every day to ensure that the larvae are fully nourished;

[0090] When cultivating queen cells, select larvae within 12 hours of hatching and transfer them into the queen cell cups, and complete the double transfer operation within 24 hours after transfer to improve the quality of the queen.

[0091] S2. Insert the queen cells of the same period into the standardized bee colony and spray the colony with a special plant liquid to unify the colony's flavor;

[0092] In this embodiment, it should be noted that the implementation steps of step S2 are:

[0093] The queen cells cultivated in step S1 are placed in the pre-selected mating group on the 6th day after capping;

[0094] The mating group is equipped with 3 honeycombs per group, including 1 capped honeycomb with a cap ratio of 60%, 1 honey and pollen honeycomb and 1 egg and larvae honeycomb, maintaining a honeycomb ratio of 1.2 to 1;

[0095] Two hours before intervention, spray 5% concentration of Yebazi plant extract evenly into the colony to unify the colony smell and avoid worker bee rejection behavior.

[0096] S3. Using vertical partitions to create a dual-zone structure, the plant extracts are simultaneously applied to blend the scents of the two zones.

[0097] In this embodiment, it should be noted that the implementation steps of step S3 are:

[0098] Use a modified twelve-frame beehive, with the interior of the hive divided into two zones by vertical queen excluders with a grid spacing of 3.8 mm plus or minus 0.1 mm. The hive doors are located on the front and back sides of the hive, with a spacing of no less than 35 cm.

[0099] On the third day after successful mating in step S2, evenly spray the surfaces of the honeycombs in both areas with 10 ml of Yebazi extract per honeycomb to completely cover the original colony odor;

[0100] At the same time, through real-time monitoring by temperature and humidity sensors in the box, the temperature of the breeding area is strictly controlled at 34.5 degrees Celsius plus or minus 0.5 degrees Celsius, and the humidity is maintained at 65% plus or minus 5%.

[0101] S4. After queen bees of the same age apply honey, they intervene in different areas and monitor worker bee behavior in real time to prevent conflicts.

[0102] In this embodiment, it should be noted that the implementation steps of step S4 are:

[0103] Select two queen bees of the same age that have successfully laid eggs in step S2 and whose age difference is no more than 24 hours, and simultaneously introduce them into the two zones in the evening;

[0104] During the operation, apply wild honey from southern Yunnan diluted to 30% concentration on the queen bee's chest, and then gently place it in the lower middle part of the honeycomb in the two zones;

[0105] An infrared monitoring system was used to continuously monitor the frequency of antennae contact among worker bees within 48 hours after intervention. If the attack behavior exceeded 5 times per minute, Yebazi extract was sprayed immediately.

[0106] S5. Dynamically adjust the size of the nest entrance according to the difference in colony strength, and exchange brood combs to balance the bee population;

[0107] In this embodiment, it should be noted that the implementation steps of step S5 are:

[0108] Dynamically adjust the nest gate size based on the difference in bee attendance between the two zones recorded on the seventh day after the intervention in step S4. When the difference in bee attendance exceeds the 15% threshold, limit the width of the nest gate to 1.5 cm for strong colonies and expand it to 2.5 cm for weak colonies.

[0109] The brood comb replacement operation is performed simultaneously: a brood comb with a capped hatch rate of not less than 80% is transferred from the strong area to the weak area, and at the same time, a brood comb with an egg density of more than 90% is transferred from the weak area to the strong area.

[0110] S6. Periodically optimize the layout of the brood spleen and supplement with special feed to maintain balanced feeding between the two areas;

[0111] In this embodiment, it should be noted that the implementation steps of step S6 are:

[0112] Adjust the layout of the brood spleen every 5 days, keeping the proportion of capped spleens at 40% to 45%, the proportion of oviparous spleens at 30% to 35%, and reserving 20% of empty spleens for egg laying;

[0113] According to the group strength data recorded in step S5, 50 grams of pollen cakes mixed with pollen, soybean powder and honey in a mass ratio of 6:3:1 are supplemented to each area every day, and 200 ml of syrup with a sugar-water ratio of 1:1 is fed simultaneously.

[0114] S7. Open worker bee channels in stages, using group interaction rates as nodes to promote swarm integration;

[0115] In this embodiment, it should be noted that the implementation steps of step S7 are:

[0116] On the 21st day after the intervention of step S4, when egg laying stabilized, partition regulation was initiated: in the first stage, 30% of the worker bee channel area was opened, and the interaction rate of the worker bees in the two areas was tested after 3 days; when the comprehensive interaction rate of antennal contact and food transfer reached more than 70%, the second stage was entered, and the channel was opened to 60% for 4 days; after reaching the interaction rate threshold again, the third stage was entered, and the channel was fully opened while retaining the physical separation.

[0117] S8. Remove the partitions and merge the two-zone bee colonies, optimize the honeycomb layout and insulation to promote the fusion of bee colonies.

[0118] In this embodiment, it should be noted that the implementation steps of step S8 are:

[0119] 15 days before the Beginning of Winter, when the outside temperature drops below 10 degrees Celsius, remove the queen excluder and merge the two-zone bee colonies;

[0120] According to the brood comb data in step S6, two capped brood combs and one honey and pollen comb are reserved and placed in the center of the box, and the distance between the two queen bees is controlled to be within the range of 8 to 10 cm;

[0121] The outside of the box is wrapped with a 3 cm thick straw insulation layer, the nest entrance is reduced to 1.0 cm, and the temperature inside the box is continuously maintained in the range of 20 degrees Celsius plus or minus 2 degrees Celsius.

[0122] Through the above steps, the present invention replaces alcohol with gradient flavoring of wild bazi plant extract to avoid the rejection behavior of worker bees; dynamic nest door regulation and the capped honeycomb replacement threshold are linked to control the balance of colony power; the partition channel is opened in stages with the worker bee interaction rate ≥70% as the switching node to achieve the gradual fusion of pheromones in the two areas; combined with the closed-loop control of the overwintering bee colony temperature and the precise management of the distance between queens, the stability of the coexistence of two queens is finally improved to more than 90%, and the upper limit of the colony power of the Chinese honeybee in southern Yunnan is broken through to a scale of 12 honeycombs.

[0123] Throughout this specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0124] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the content of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A method for breeding double queen bees in southern Yunnan, characterized in that: The following steps are involved: S1. Select local high-yield and disease-resistant bee species and cultivate high-quality queen bees through enhanced nutrition and multiple bee transplanting operations; S2. Insert the queen cells of the same period into the standardized bee colony and spray the colony with a special plant liquid to unify the colony's flavor; S3. Using vertical partitions to create a dual-zone structure, the plant extracts are simultaneously applied to blend the scents of the two zones. S4. After queen bees of the same age apply honey, they intervene in different areas and monitor worker bee behavior in real time to prevent conflicts. S5. Dynamically adjust the size of the nest entrance according to the difference in colony strength, and exchange brood combs to balance the bee population; S6. Periodically optimize the layout of the brood spleen and supplement with special feed to maintain balanced feeding between the two areas; S7. Open worker bee channels in stages, using group interaction rates as nodes to promote swarm integration; S8. Remove the partitions and merge the two-zone bee colonies, optimize the honeycomb layout and insulation to promote the fusion of bee colonies.

2. The method for breeding double queen bees in southern Yunnan according to claim 1, characterized in that: The implementation steps of step S1 are: Select queen bees with stable egg-laying ability, an average daily egg-laying capacity of no less than 1,100 eggs, and strong disease resistance from high-quality local bee colonies in southern Yunnan as mother bees, and cultivate queen cells through artificial transfer; The queen bee is introduced into the strong colony 45 days in advance for intensive feeding, and is fed 100 ml of pollen paste mixed with honey at a mass ratio of 1 to 1.5 every day to ensure that the larvae are fully nourished. When cultivating queen cells, select larvae within 12 hours of hatching and transfer them into the queen cell cups, and complete the double transfer operation within 24 hours after transfer to improve the quality of the queen.

3. The method for breeding double queen bees in southern Yunnan according to claim 2, characterized in that: The implementation steps of step S2 are: The queen cells cultivated in step S1 are placed in the pre-selected mating group on the 6th day after capping; The mating group is equipped with 3 honeycombs per group, including 1 capped honeycomb with a cap ratio of 60%, 1 honey and pollen honeycomb and 1 egg and larvae honeycomb, maintaining a honeycomb ratio of 1.2 to 1; Two hours before intervention, spray 5% concentration of Yebazi plant extract evenly into the colony to unify the colony smell and avoid worker bee rejection behavior.

4. The method for breeding double queen bees in southern Yunnan according to claim 3, characterized in that: The implementation steps of step S3 are: Use a modified twelve-frame beehive, with the interior of the hive divided into two zones by vertical queen excluders with a grid spacing of 3.8 mm plus or minus 0.1 mm. The hive doors are located on the front and back sides of the hive, with a spacing of no less than 35 cm. On the third day after successful mating in step S2, evenly spray the surfaces of the honeycombs in both areas with 10 ml of Yebazi extract per honeycomb to completely cover the original colony odor; At the same time, through real-time monitoring by temperature and humidity sensors in the box, the temperature of the breeding area is strictly controlled at 34.5 degrees Celsius plus or minus 0.5 degrees Celsius, and the humidity is maintained at 65% plus or minus 5%.

5. The method for breeding double queen bees in southern Yunnan according to claim 4, characterized in that: The implementation steps of step S4 are: Select two queen bees of the same age that have successfully laid eggs in step S2 and whose age difference is no more than 24 hours, and simultaneously introduce them into the two zones in the evening; During the operation, apply wild honey from southern Yunnan diluted to 30% concentration on the queen bee's chest, and then gently place it in the lower middle part of the honeycomb in the two zones; An infrared monitoring system was used to continuously monitor the frequency of antennae contact among worker bees within 48 hours after intervention. If the attack behavior exceeded 5 times per minute, Yebazi extract was sprayed immediately.

6. The method for breeding double queen bees in southern Yunnan according to claim 5, characterized in that: The implementation steps of step S5 are: Dynamically adjust the nest gate size based on the difference in bee attendance between the two zones recorded on the seventh day after the intervention in step S4. When the difference in bee attendance exceeds the 15% threshold, limit the width of the nest gate to 1.5 cm for strong colonies and expand it to 2.5 cm for weak colonies. The brood comb replacement operation is performed simultaneously: a brood comb with a capped hatch rate of not less than 80% is transferred from the strong area to the weak area, and at the same time, a brood comb with an egg density of more than 90% is transferred from the weak area to the strong area.

7. The method for breeding double queen bees in southern Yunnan according to claim 6, characterized in that: The implementation steps of step S6 are: Adjust the layout of the brood spleen every 5 days, keeping the proportion of capped spleens at 40% to 45%, the proportion of oviparous spleens at 30% to 35%, and reserving 20% of empty spleens for egg laying; According to the group strength data recorded in step S5, 50 grams of pollen cakes mixed with pollen, soybean powder and honey in a mass ratio of 6:3:1 are supplemented to each area every day, and 200 ml of syrup with a sugar-water ratio of 1:1 is fed simultaneously.

8. The method for breeding double queen bees in southern Yunnan according to claim 7, characterized in that: The implementation steps of step S7 are: On the 21st day after the intervention of step S4, when egg laying stabilized, partition regulation was initiated: in the first stage, 30% of the worker bee channel area was opened, and the interaction rate of the worker bees in the two areas was tested after 3 days; when the comprehensive interaction rate of antennal contact and food transfer reached more than 70%, the second stage was entered, and the channel was opened to 60% for 4 days; after reaching the interaction rate threshold again, the third stage was entered, and the channel was fully opened while retaining the physical separation.

9. The method for breeding double queen bees in southern Yunnan according to claim 7, characterized in that: The implementation steps of step S8 are: 15 days before the Beginning of Winter, when the outside temperature drops below 10 degrees Celsius, remove the queen excluder and merge the two-zone bee colonies; According to the brood comb data in step S6, two capped brood combs and one honey and pollen comb are reserved and placed in the center of the box, and the distance between the two queen bees is controlled to be within the range of 8 to 10 cm; The outside of the box is wrapped with a 3 cm thick straw insulation layer, the nest entrance is reduced to 1.0 cm, and the temperature inside the box is continuously maintained in the range of 20 degrees Celsius plus or minus 2 degrees Celsius.