Compound nutrition preparation and application thereof

Abstract

The invention discloses a compound nutrition preparation and application thereof, and relates to the technical field of health food, and the compound nutrition preparation comprises glutamine, arginine, Omega-3 fatty acid, vitamin A, vitamin C, vitamin E, zinc and selenium. Animal experiments prove that the traditional Chinese medicine composition has a relatively good promotion effect on wound healing, and can obviously increase the thickness of granulation tissue of a wound surface and the length of newborn epithelization, promote generation and deposition of collagenous fibers of the wound surface tissue, promote angiogenesis of the wound surface, improve the nutritional status of a body, inhibit inflammation to a certain extent and improve the immune function of rats; clinical tests prove that the compound nutrition preparation can accelerate the postoperative wound healing speed of a patient, strengthen the T lymphocyte function, reduce the inflammation and stress index level and increase the serum PA level. The compound nutrition preparation can accelerate wound healing after acute trauma, improve body nutrition status, increase immune function, relieve systemic inflammatory response and shorten post-operation hospitalization time.

Description

Technical Field

[0001] The present invention relates to the technical field of health food, in particular to a compound nutritional preparation and application thereof. Background Art

[0002] Modern clinical nutritional research confirms that macronutrients play a dual role in wound repair: they provide the essential energy matrix for tissue regeneration and serve as structural building blocks for cell proliferation. Notably, modern clinical nutritional therapy focuses on supplementing nutrients with specific biological effects to maintain tissue and organ function, enhance immune responses, and suppress excessive inflammation. Immunomodulatory nutrients such as arginine, omega-3 polyunsaturated fatty acids, and glutamine; antioxidant vitamins such as vitamins A, C, and E; and antioxidant minerals such as zinc and selenium demonstrate unique therapeutic value in wound repair through multi-target mechanisms of action. Their effectiveness is not only reflected in fundamental aspects such as shortening wound healing cycles and optimizing protein metabolism, but also significantly reduces the incidence of postoperative complications by regulating immune homeostasis.

[0003] Daily supplementation of 20g of glutamine can significantly shorten wound closure time in trauma patients, improve nitrogen balance and protein metabolism, reduce insulin resistance, and lower the incidence of infectious complications. Arginine, a precursor of nitric oxide and proline, is essential for collagen synthesis. Daily supplementation of 9g of arginine can promote protein synthesis in trauma patients, increase collagen fiber content in wound scars, improve nitrogen balance, and accelerate wound healing and recovery. Omega-3 fatty acids can significantly enhance immunity and reduce the risk of infection by downregulating inflammatory responses, increasing the CD4+ / CD8+ T cell ratio, and enhancing B cell-specific antibody production. The recommended supplementation dose is 1g / day. Furthermore, supplementation with 30mg / day of zinc and 100μg / day of selenium can enhance immunity and play a key role in the proliferation and remodeling phases of wound healing. They stimulate related enzymes to promote collagen synthesis, fibroblast proliferation, and epithelialization. Supplementation with 1500μg RAE / day of vitamin A and 200mg α-TE / day of vitamin E has shown positive effects on wound healing by regulating inflammatory responses. During wound healing, supplementation with 500mg / day of vitamin C not only promotes cell migration and transformation but also enhances collagen synthesis and antioxidant responses, while also playing multiple roles in angiogenesis.

[0004] The effects of multiple nutrients are not simply superimposed, but more likely to be synergistic, and the effects are often better than single nutrient supplementation. However, there is currently a lack of a composite nutritional preparation that integrates multiple nutrients and enables the respective nutrients to work synergistically with each other. Based on this, the present invention is proposed. Summary of the Invention

[0005] The present invention aims to provide a compound nutritional preparation and its application to enhance the patient's immune response and inhibit excessive inflammatory response, promote wound healing, and reduce the risk of infection.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A first aspect of the present invention provides a compound nutritional preparation, which includes immunonutrients, antioxidant vitamins and antioxidant minerals, wherein the immunonutrients include glutamine, arginine and Omega-3 fatty acids, the antioxidant vitamins include vitamin A, vitamin C and vitamin E, and the antioxidant minerals include zinc and selenium.

[0008] Furthermore, calculated per 100 grams, it includes 49.88g of glutamine, 22.446g of arginine, 2.494g of Omega-3 fatty acids, 0.003741g of vitamin A, 1.247g of vitamin C, 0.4988g of vitamin E, 0.07482g of zinc and 0.0002494g of selenium.

[0009] A second aspect of the present invention provides a use of a compound nutritional preparation for accelerating wound healing after acute trauma.

[0010] Furthermore, the compound nutritional preparation plays a role in increasing the thickness of wound granulation tissue and the length of new epithelialization.

[0011] Furthermore, the compound nutritional preparation plays a role in promoting the generation and deposition of collagen fibers in wound tissue.

[0012] Furthermore, the compound nutritional preparation plays a role in promoting angiogenesis in the wound surface.

[0013] Furthermore, the compound nutritional preparation plays a role in improving the nutritional status of the body, inhibiting inflammation and enhancing immune function.

[0014] The principle and beneficial effects of this technical solution:

[0015] The present invention discloses a compound nutritional preparation, and its design principle is mainly based on the synergistic effect between special nutrients. Supplementing nutrients with specific biological effects can maintain tissue and organ function, enhance immune response and inhibit excessive inflammatory response, promote wound healing, and reduce infection risk. The supplementation of immunonutrients (glutamine, arginine, Omega-3 fatty acids) can improve wound healing and reduce the occurrence of postoperative complications and infectious diseases; antioxidant vitamins (vitamins A, C, E) and minerals (zinc and selenium) also play an important role in wound healing. Design a compound nutritional preparation comprising immunonutrients (glutamine, arginine, Omega-3 fatty acids) and antioxidant vitamins (vitamins A, C, E) and minerals (zinc and selenium).

[0016] Animal experiments have shown that it has a good promoting effect on wound healing, can significantly improve the wound healing rate, can significantly increase the thickness of wound granulation tissue and the length of new epithelialization, promote the formation and deposition of collagen fibers in wound tissue, promote angiogenesis in the wound, improve the body's nutritional status, inhibit inflammation to a certain extent, and enhance the immune function of rats;

[0017] Clinical trials have shown that this compound nutritional preparation can accelerate postoperative wound healing, enhance T lymphocyte function, reduce inflammation and stress markers, and increase serum PA levels. This suggests that the compound nutritional preparation can accelerate wound healing after acute trauma, improve nutritional status, enhance immune function, reduce systemic inflammatory responses, and shorten postoperative hospital stays. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Figure A is a representative image of the wound healing status of rats at different time points after trauma modeling; Figure B is a schematic diagram of the effect of compound nutritional preparation intervention on the wound healing rate of traumatic rats;

[0019] Figure 2 A is a schematic diagram of HE staining of wound tissue on days 3, 7, and 14, with wound boundaries as black arrows; B is a schematic diagram of quantitative analysis of granulation tissue thickness in each group on day 14 (n=5); C is a schematic diagram of quantitative analysis of the length of newly epithelialized wounds in each group on day 14 (n=5);

[0020] Figure 3 A is a schematic diagram of Masson staining of wound tissue on days 3, 7, and 14; B is a schematic diagram of quantitative analysis of collagen deposition on day 14 (n=5); C is a schematic diagram of serum Hyp detection results;

[0021] Figure 4 A is a schematic diagram of immunohistochemical staining of wound tissue on days 3, 7, and 14; B is a schematic diagram of quantitative analysis of angiogenesis on day 14 (n=5);

[0022] Figure 5 A is a schematic diagram of serum ALB levels; B is a schematic diagram of serum PA levels; C is a schematic diagram of serum TP levels;

[0023] Figure 6 A is a schematic diagram of serum TNF-α levels; B is a schematic diagram of serum IL-1β levels; C is a schematic diagram of serum IL-6 levels; D is a schematic diagram of serum IL-10 levels;

[0024] Figure 7 A is a schematic diagram of serum IgA levels; B is a schematic diagram of serum IgG levels; C is a schematic diagram of serum IgM levels;

[0025] Figure 8 This is a schematic diagram of the effects of compound nutritional preparations on immune organ indexes;

[0026] exist Figure 1-7 Middle: a: P < 0.05, compared with the CON group; b: P < 0.05, compared with the WP group; c: P < 0.05, compared with the LDF group;

[0027] Figure 9 This is a comparison chart of postoperative wound healing;

[0028] Figure 10 Box diagram of changes in immune function indicators (A: CD3 + Changes of T cells over time; B: CD4 + T cell changes over time);

[0029] Figure 11 Box plots of changes in inflammatory indicators (A: changes in NLR over time; B: changes in CRP over time; C: changes in TNF-α over time; D: changes in IL-6 over time);

[0030] Figure 12 Schematic diagram of PA changes over time. DETAILED DESCRIPTION

[0031] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments:

[0032] Example 1

[0033] A compound nutritional preparation and its application, the specific gravity per 100 grams is as follows:

[0034]

[0035] Experimental Example 1

[0036] Animal experiment: Study on the effect of compound nutritional preparation on wound healing after acute trauma in rats

[0037] The control group, whey protein group, low-dose compound nutritional preparation group and high-dose compound nutritional preparation group were set up, among which the control group was gavage with normal saline (equal volume)

[0038] Whey protein group: Oral administration of whey protein (6.682g / kg)

[0039] Low-dose compound nutritional preparation group: Oral administration of low-dose compound nutritional preparation (3.341 g / kg)

[0040] The high-dose compound nutritional preparation group was administered with a high-dose compound nutritional preparation (6.682 g / kg) by gavage.

[0041] The wound healing status was recorded on the 3rd, 7th and 14th days after surgery.

[0042] refer to Figure 1 , compound nutritional preparations have a good promoting effect on wound healing, and there is a certain dose-dependent effect.

[0043] refer to Figure 2 HE staining was used to evaluate the histopathological changes of the wounds of rats in each group. The intervention of compound nutritional preparations could significantly increase the thickness of granulation tissue and the length of new epithelialization on the wound surface.

[0044] refer to Figure 3 Masson staining and Hyp level were used to evaluate the effect of compound nutritional preparation intervention on the collagen fiber formation in wound tissue. The compound nutritional preparation intervention promoted the formation and deposition of collagen fibers in wound tissue, and the effect was dose-related.

[0045] refer to Figure 4 Immunohistochemical staining was used to mark and detect the expression of CD31 to evaluate the effect of compound nutritional preparation intervention on angiogenesis of wound tissue. The compound nutritional preparation intervention significantly promoted angiogenesis of the wound, and this effect had a certain dose-dependent effect.

[0046] refer to Figure 5 The intervention of compound nutritional preparations significantly improved the nutritional status of the body, and this effect had a certain dose-dependent effect.

[0047] refer to Figure 6 Administration of whey protein or compound nutritional preparations can inhibit inflammation to a certain extent, with the HDF group having the best effect. The effect of compound nutritional preparations during the inflammatory period is dose-dependent.

[0048] refer to Figure 7 , compound nutritional preparation intervention can improve the immune function of rats to a certain extent.

[0049] refer to Figure 8 There was no statistically significant difference in the effects on organ indexes.

[0050] Experimental Example 2

[0051] Clinical trial: Study on the effect of compound nutritional preparation on wound healing in patients with open surgical trauma

[0052] Research subjects: The research subjects are patients who are going to undergo open surgery for trauma treatment in cardiac surgery.

[0053] Study Design:

[0054] A randomized controlled study was conducted, with 30 patients in the experimental group and 30 patients in the control group. From the first to the seventh day after surgery, the two groups of patients received enteral nutrition intervention through different feeding routes (tube feeding / oral) according to the patients' conditions. The control group was given a conventional enteral nutrition formula, and the experimental group was given an enteral nutrition formula containing a compound nutritional preparation (glutamine 20g, arginine 9g, omega-3 fatty acid 1g, vitamin A 1500μg, vitamin C 500mg, vitamin E 200mg, zinc 30mg, selenium 100μg). The nutritional intervention of the two groups adopted an equal energy and equal protein feeding strategy.

[0055] Research Results:

[0056] Comparison of wound healing and hospitalization time between the two groups

[0057] The wound healing was evaluated by REEDA scoring on the 7th and 14th days after surgery, as shown in Table 1. Figure 9 Table 1 shows the comparison of REEDA scores between the experimental and control groups. The results showed that on days 7 and 14 after surgery, the experimental group had lower REEDA scores than the control group. The postoperative hospital stay in the experimental group was significantly shorter than that in the control group. These results indicate that the addition of compound nutritional preparations can effectively accelerate wound healing and shorten postoperative hospital stays.

[0058] Table 1 Comparison of postoperative wound REEDA scores and postoperative hospitalization time of subjects

[0059]

[0060] Note: Normally distributed data are expressed as mean ± standard deviation.

[0061] Comparison of immune function indicators between the two groups

[0062] Refer to Table 2 and Figure 10 Compared with the two groups, no significant differences were observed in any indicators before surgery and on the third day after surgery. On the seventh day after surgery, the CD3+ and CD4+ T cell counts in the experimental group were significantly higher than those in the control group, while there were no statistically significant differences in CD8+ cell counts and CD4+ / CD8+ ratios between the two groups.

[0063] Table 2 Comparison of immune function indicators between the two groups at different time points

[0064]

[0065]

[0066] Note: Normally distributed data are expressed as mean ± standard deviation; non-normally distributed data are expressed as median Med (P 25 , P 75 ).

[0067] Comparison of inflammation and stress indicators between the two groups

[0068] Refer to Table 3 and Figure 11 No significant differences were observed in any of the indicators before and after surgery on day 3. On day 7 after surgery, the levels of TNF-α, CRP, IL-6, and NLR in the experimental group were significantly lower than those in the control group, while there was no statistically significant difference in WBC.

[0069] Table 3 Comparison of inflammation and stress indicators between the two groups at different time points

[0070]

[0071] Note: Normally distributed data are expressed as mean ± standard deviation; non-normally distributed data are expressed as median Med (P 25 , P 75 ). NLR, neutrophil-to-lymphocyte ratio; WBC, white blood cell count; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6.

[0072] Comparison of nutritional status indicators between the two groups

[0073] Refer to Table 4 and Figure 12 Postoperative nutritional status indicators in both the experimental and control groups significantly decreased due to surgical stress, with no significant differences observed before surgery or on day 3 after surgery. On day 7 after surgery, PA levels in the experimental group were significantly higher than those in the control group, indicating superior PA recovery in the experimental group. There were no statistically significant differences in ALB and TP levels.

[0074] Table 4 Comparison of nutritional status indicators between the two groups at different time points

[0075]

[0076] Note: Normally distributed data are expressed as mean ± standard deviation; non-normally distributed data are expressed as median Med (P 25 , P 75 ). ALB, albumin; PA, prealbumin; TP, total protein.

[0077] Research Results:

[0078] This compound nutritional preparation can accelerate postoperative wound healing, enhance T lymphocyte (CD3+, CD4+) function, reduce levels of inflammatory and stress indicators such as NLR, CRP, TNF-α, and IL-6, and increase serum PA levels. This suggests that the compound nutritional preparation can accelerate wound healing after acute trauma, improve nutritional status, enhance immune function, reduce systemic inflammatory responses, and shorten postoperative hospital stays.

[0079] It should be understood that the present invention is not limited to the specific configurations and processes described above and illustrated in the figures. For the sake of brevity, detailed descriptions of known methods are omitted. In the present invention, features described and / or illustrated in one embodiment may be used in the same or similar manner in one or more other embodiments, and / or combined with or substituted for features of other embodiments.

[0080] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations to the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A compound nutritional preparation, characterized in that: The nutritional preparation includes immunonutrients, antioxidant vitamins and antioxidant minerals, wherein the immunonutrients include glutamine, arginine and Omega-3 fatty acids, the antioxidant vitamins include vitamin A, vitamin C and vitamin E, and the antioxidant minerals include zinc and selenium.

2. A compound nutritional preparation according to claim 1, characterized in that: Calculated by weight per 100 grams, it includes 49.88g of glutamine, 22.446g of arginine, 2.494g of Omega-3 fatty acids, 0.003741g of vitamin A, 1.247g of vitamin C, 0.4988g of vitamin E, 0.07482g of zinc and 0.0002494g of selenium.

3. The use of a compound nutritional preparation to accelerate wound healing after acute trauma.

4. An application according to claim 3, characterized in that: The compound nutritional preparation plays a role in increasing the thickness of wound granulation tissue and the length of new epithelialization.

5. The application according to claim 3, characterized in that: The compound nutritional preparation plays a role in promoting the generation and deposition of collagen fibers in wound tissue.

6. The application according to claim 3, characterized in that: The compound nutritional preparation plays a role in promoting angiogenesis of the wound surface.

7. The use according to claim 3, characterized in that: The compound nutritional preparation plays a role in improving the nutritional status of the body, inhibiting inflammation and enhancing immune function.

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