Sheep liver peptide nutrition formula based on low-temperature enzymolysis technology and efficient extraction process
Through low-temperature enzymatic lysis technology and scientific formula, the problem of low extraction efficiency of traditional sheep liver peptides has been solved, and high-quality sheep liver peptides have been effectively extracted, which is suitable for health foods, pharmaceutical cosmetics and other fields.
Patent Information
- Application Number
- CN202510616091.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-14
- Publication Date
- 2025-08-15
AI Technical Summary
The traditional sheep hepatic peptide extraction method is inefficient and has a large loss of active ingredients, making it difficult to meet the market's demand for high-quality sheep hepatic peptide products.
Low-temperature enzymatic lysis technology is used to decompose proteins in sheep liver under low temperature conditions, combine nutritional formulas of vitamin C, vitamin E, zinc and selenium, and carry out enzymatic lysis reactions at 30-40℃ through specific enzymes, followed by enzyme decomposition, separation and purification, and drying to make powder to form small molecule peptides and amino acids that are easy to absorb by the human body.
Effectively retain the natural nutritional activity of sheep liver, improve product quality and efficacy, simplify production processes, reduce costs, comply with food safety standards, and is suitable for health foods, pharmaceutical cosmetics and other fields.
Abstract
Description
Technical Field
[0001] The invention belongs to the field of biotechnology, and in particular relates to a sheep liver peptide nutritional formula and an efficient extraction process based on low-temperature enzymatic hydrolysis technology. Background Art
[0002] Extracting functional ingredients from animal liver and developing them into nutritional supplements has become a hot topic in modern biotechnology and nutritional research. Sheep liver, a nutrient-rich ingredient rich in protein, vitamin A, iron, zinc, and other nutrients, has shown significant benefits in improving vision, boosting immunity, assisting with anemia, and promoting metabolism.
[0003] However, traditional extraction methods often have problems such as low efficiency and large loss of active ingredients, making it difficult to meet the market demand for high-quality sheep liver peptide products.
[0004] Therefore, it is of great practical significance to develop a nutritional formula and efficient extraction process of sheep liver peptide based on low-temperature enzymatic hydrolysis technology. Summary of the Invention
[0005] The purpose of the present invention is to overcome the above-mentioned problems existing in traditional technologies and provide a sheep liver peptide nutritional formula and an efficient extraction process based on low-temperature enzymatic hydrolysis technology.
[0006] In order to achieve the above technical objectives and the above technical effects, the present invention is implemented through the following technical solutions:
[0007] The present invention provides a sheep liver peptide nutritional formula based on low-temperature enzymatic hydrolysis technology. The main component of the sheep liver peptide nutritional formula is sheep liver peptide, which accounts for 50-70wt% of the sheep liver peptide. The sheep liver peptide is extracted by low-temperature enzymatic hydrolysis technology; low-temperature enzymatic hydrolysis technology uses specific enzymes to decompose the protein in the sheep liver under a low-temperature environment, converting it into small-molecule peptides and amino acids. The advantage of this technology is that the low temperature condition can effectively prevent the damage of heat-sensitive nutrients in the sheep liver by high temperature, thereby preserving the natural nutritional activity of the sheep liver to the greatest extent. At the same time, the enzymatic hydrolysis process is highly specific and efficient, and can accurately decompose the protein into small-molecule peptide segments that are easily absorbed by the human body.
[0008] The antioxidant system of this sheep liver peptide nutritional formula includes vitamin C and vitamin E;
[0009] The trace elements of this sheep liver peptide nutritional formula include zinc and selenium.
[0010] Furthermore, in the above-mentioned sheep liver peptide nutritional formula, the added amount of vitamin C is 200-500 mg / 100 g of sheep liver peptide.
[0011] Furthermore, in the above-mentioned sheep liver peptide nutritional formula, the added amount of vitamin E is 50-150 mg / 100 g of sheep liver peptide.
[0012] Furthermore, in the above-mentioned sheep liver peptide nutritional formula, the added amount of zinc is 5-15 mg / 100 g sheep liver peptide.
[0013] Furthermore, in the above-mentioned sheep liver peptide nutritional formula, the added amount of selenium is 20-50 μg / 100g sheep liver peptide.
[0014] The present invention also provides a high-efficiency extraction process of sheep liver peptide based on low-temperature enzymatic hydrolysis technology, comprising the following steps:
[0015] S1. Raw material pretreatment: Select fresh, high-quality sheep liver as raw material, wash the liver, remove the connective tissue and impurities on the surface, and then cut it into small pieces for subsequent processing;
[0016] S2. Low-temperature enzymatic hydrolysis: Add an appropriate amount of purified water to the pretreated sheep liver block and stir evenly to prepare a liver tissue homogenate; adjust the pH value of the homogenate to 6.5-7.5, then add the enzyme preparation and perform enzymatic hydrolysis at a low temperature of 30-40°C for 4-8 hours;
[0017] S3, enzyme inactivation treatment: After the enzymatic hydrolysis reaction is completed, the enzymatic hydrolysis solution is quickly heated to 80-90°C and maintained for 10-15 minutes to inactivate the enzyme and terminate the enzymatic hydrolysis reaction;
[0018] S4, separation and purification: using centrifugation and filtration to remove residues and insoluble impurities in the enzymatic hydrolyzate to obtain a clarified sheep liver peptide solution; using ultrafiltration and chromatography technology to separate and purify the sheep liver peptide solution to remove salt, small molecule impurities and large molecule proteins to obtain a purified sheep liver peptide solution;
[0019] S5, concentration and drying: concentrating the purified sheep liver peptide solution to remove water therein and increase the concentration of the sheep liver peptide;
[0020] S6. Drying and powdering: Use spray drying or freeze drying to dry the concentrated sheep liver peptide solution into a powdered product.
[0021] Furthermore, in step S2, the enzyme preparation is papain or trypsin.
[0022] Furthermore, in step S2, continuous stirring is required during the enzymatic hydrolysis process to ensure that the enzyme is fully in contact with the sheep liver and to improve the enzymatic hydrolysis efficiency.
[0023] The beneficial effects of the present invention are:
[0024] 1. The proposed sheep liver peptide nutritional formula and efficient extraction process based on low-temperature enzymatic hydrolysis technology are innovative in both technical principles and process steps. Compared with traditional extraction methods, this technology can better preserve the nutrients and biological activity of sheep liver, improving the quality and efficacy of the product.
[0025] 2. By optimizing the enzymatic hydrolysis conditions and process parameters, the extraction process of the present invention is highly efficient and can obtain high-purity sheep liver peptide in a relatively short period of time. At the same time, the process is simple to operate and is easy to industrialize, which can reduce production costs and improve production efficiency.
[0026] 3. The extraction process of this invention uses low-temperature enzymatic hydrolysis technology, which avoids damage and contamination to sheep liver caused by high temperatures and chemical reagents, thereby ensuring product safety. At the same time, the nutritional formula of the product has been rigorously scientifically demonstrated and experimentally verified, and complies with relevant national food safety standards.
[0027] 4. The sheep liver peptide nutritional formula developed by the present invention has multiple functions that can meet the health needs of different groups of people. For example, the product has significant effects in improving vision, enhancing immunity, assisting in improving anemia, and promoting metabolism. It has excellent auxiliary treatment and health care effects for patients with liver disease, people with low immunity, and anemia.
[0028] 5. The present invention's sheep liver peptide nutritional formula and efficient extraction process, based on low-temperature enzymatic hydrolysis technology, has broad application prospects. The product can be used as a nutritional supplement in health foods and functional foods, and as a raw material in the pharmaceutical and cosmetic industries. With rising health awareness and demand for high-quality nutritional products, sheep liver peptide products have enormous market potential and are expected to become an emerging hot topic in the biotechnology and nutrition and health fields.
[0029] Of course, any product implementing the present invention does not necessarily need to achieve all of the above advantages at the same time. DETAILED DESCRIPTION
[0030] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.
[0031] The relevant specific embodiments of the present invention are as follows:
[0032] Example 1
[0033] The present embodiment provides a sheep liver peptide nutritional formula based on low-temperature enzymatic hydrolysis technology, the main component of the sheep liver peptide nutritional formula is sheep liver peptide, the proportion of sheep liver peptide is 60wt%, and the sheep liver peptide is extracted by low-temperature enzymatic hydrolysis technology. Low-temperature enzymatic hydrolysis technology uses specific enzymes to decompose the protein in sheep liver under low-temperature conditions and convert it into small-molecule peptides and amino acids. The advantage of this technology is that low-temperature conditions can effectively avoid the destruction of heat-sensitive nutrients in sheep liver by high temperature, and retain the natural nutritional activity of sheep liver to the greatest extent. At the same time, the enzymatic hydrolysis process is highly specific and efficient, and can accurately decompose protein into small-molecule peptide segments that are easily absorbed by the human body.
[0034] The antioxidant system of the sheep liver peptide nutritional formula includes vitamin C and vitamin E. The added amount of vitamin C is 350 mg / 100 g of sheep liver peptide, and the added amount of vitamin E is 100 mg / 100 g of sheep liver peptide.
[0035] The trace elements of this sheep liver peptide nutritional formula include zinc and selenium. The added amount of zinc is 10 mg / 100 g of sheep liver peptide, and the added amount of selenium is 35 μg / 100 g of sheep liver peptide.
[0036] This embodiment provides an efficient extraction process of sheep liver peptide based on low-temperature enzymatic hydrolysis technology, comprising the following steps:
[0037] S1. Raw material pretreatment: Select fresh, high-quality sheep liver as raw material, wash the liver, remove the connective tissue and impurities on the surface, and then cut it into small pieces for subsequent processing;
[0038] S2. Low-temperature enzymatic hydrolysis: Add an appropriate amount of purified water to the pretreated sheep liver blocks and stir evenly to prepare a liver tissue homogenate; adjust the pH value of the homogenate to 7.0, and then add an enzyme preparation, which is papain; carry out the enzymatic hydrolysis reaction at a low temperature of 35°C for 6 hours; continuous stirring is required during the enzymatic hydrolysis process to ensure sufficient contact between the enzyme and the sheep liver to improve the enzymatic hydrolysis efficiency.
[0039] S3. Enzyme inactivation: After the enzymatic hydrolysis reaction is completed, the hydrolyzate is quickly heated to 85°C and maintained for 12 minutes to inactivate the enzyme and terminate the enzymatic hydrolysis reaction. The purpose of this step is to prevent the enzyme from continuing to act, resulting in excessive decomposition of the peptide fragments and affecting the quality and activity of the product.
[0040] S4. Separation and purification: Use centrifugation and filtration to remove the residue and insoluble impurities in the enzymatic hydrolyzate to obtain a clarified sheep liver peptide solution; use ultrafiltration and chromatography technology to separate and purify the sheep liver peptide solution to remove salt, small molecule impurities and large molecule proteins to obtain a purified sheep liver peptide solution.
[0041] S5. Concentration and drying: Concentrate the purified sheep liver peptide solution to remove water and increase the concentration of the sheep liver peptide.
[0042] S6. Drying and powdering: The concentrated sheep liver peptide solution is dried into a powdered product by spray drying.
[0043] Example 2
[0044] The present embodiment provides a sheep liver peptide nutritional formula based on low-temperature enzymatic hydrolysis technology, the main component of the sheep liver peptide nutritional formula is sheep liver peptide, the proportion of sheep liver peptide is 50wt%, and the sheep liver peptide is extracted by low-temperature enzymatic hydrolysis technology. Low-temperature enzymatic hydrolysis technology uses specific enzymes to decompose the protein in sheep liver under low-temperature conditions and convert it into small-molecule peptides and amino acids. The advantage of this technology is that low-temperature conditions can effectively avoid the destruction of heat-sensitive nutrients in sheep liver by high temperature, and retain the natural nutritional activity of sheep liver to the greatest extent. At the same time, the enzymatic hydrolysis process is highly specific and efficient, and can accurately decompose protein into small-molecule peptide segments that are easily absorbed by the human body.
[0045] The antioxidant system of the sheep liver peptide nutritional formula includes vitamin C and vitamin E. The added amount of vitamin C is 200 mg / 100 g of sheep liver peptide, and the added amount of vitamin E is 150 mg / 100 g of sheep liver peptide.
[0046] The trace elements of this sheep liver peptide nutritional formula include zinc and selenium. The added amount of zinc is 5 mg / 100 g of sheep liver peptide, and the added amount of selenium is 50 μg / 100 g of sheep liver peptide.
[0047] This embodiment provides an efficient extraction process of sheep liver peptide based on low-temperature enzymatic hydrolysis technology, comprising the following steps:
[0048] S1. Raw material pretreatment: Select fresh, high-quality sheep liver as raw material, wash the liver, remove the connective tissue and impurities on the surface, and then cut it into small pieces for subsequent processing;
[0049] S2. Low-temperature enzymatic hydrolysis: Add an appropriate amount of purified water to the pretreated sheep liver blocks and stir evenly to prepare a liver tissue homogenate; adjust the pH value of the homogenate to 6.5, and then add an enzyme preparation, which is trypsin; carry out the enzymatic hydrolysis reaction at a low temperature of 40°C for 4 hours; continuous stirring is required during the enzymatic hydrolysis process to ensure sufficient contact between the enzyme and the sheep liver to improve the enzymatic hydrolysis efficiency.
[0050] S3. Enzyme inactivation: After the enzymatic hydrolysis reaction is completed, the hydrolyzate is quickly heated to 80°C and maintained for 15 minutes to inactivate the enzyme and terminate the enzymatic hydrolysis reaction. The purpose of this step is to prevent the enzyme from continuing to act, resulting in excessive decomposition of the peptide fragments and affecting the quality and activity of the product.
[0051] S4. Separation and purification: Use centrifugation and filtration to remove the residue and insoluble impurities in the enzymatic hydrolyzate to obtain a clarified sheep liver peptide solution; use ultrafiltration and chromatography technology to separate and purify the sheep liver peptide solution to remove salt, small molecule impurities and large molecule proteins to obtain a purified sheep liver peptide solution.
[0052] S5. Concentration and drying: Concentrate the purified sheep liver peptide solution to remove water and increase the concentration of the sheep liver peptide.
[0053] S6. Drying and powdering: freeze-drying the concentrated sheep liver peptide solution into a powdered product.
[0054] Example 3
[0055] The present embodiment provides a sheep liver peptide nutritional formula based on low-temperature enzymatic hydrolysis technology, the main component of the sheep liver peptide nutritional formula is sheep liver peptide, the proportion of sheep liver peptide is 70wt%, and the sheep liver peptide is extracted by low-temperature enzymatic hydrolysis technology. Low-temperature enzymatic hydrolysis technology uses specific enzymes to decompose the protein in sheep liver under low-temperature conditions and convert it into small-molecule peptides and amino acids. The advantage of this technology is that low-temperature conditions can effectively avoid the destruction of heat-sensitive nutrients in sheep liver by high temperature, and retain the natural nutritional activity of sheep liver to the greatest extent. At the same time, the enzymatic hydrolysis process is highly specific and efficient, and can accurately decompose protein into small-molecule peptide segments that are easily absorbed by the human body.
[0056] The antioxidant system of the sheep liver peptide nutritional formula includes vitamin C and vitamin E. The added amount of vitamin C is 500 mg / 100 g of sheep liver peptide, and the added amount of vitamin E is 50 mg / 100 g of sheep liver peptide.
[0057] The trace elements of this sheep liver peptide nutritional formula include zinc and selenium, with the added amount of zinc being 15 mg / 100 g of sheep liver peptide and the added amount of selenium being 20 μg / 100 g of sheep liver peptide.
[0058] This embodiment provides an efficient extraction process of sheep liver peptide based on low-temperature enzymatic hydrolysis technology, comprising the following steps:
[0059] S1. Raw material pretreatment: Select fresh, high-quality sheep liver as raw material, wash the liver, remove the connective tissue and impurities on the surface, and then cut it into small pieces for subsequent processing;
[0060] S2. Low-temperature enzymatic hydrolysis: Add an appropriate amount of purified water to the pretreated sheep liver blocks and stir evenly to prepare a liver tissue homogenate; adjust the pH value of the homogenate to 7.5, and then add an enzyme preparation, such as papain or trypsin; carry out the enzymatic hydrolysis reaction at a low temperature of 30°C for 8 hours; during the enzymatic hydrolysis process, continuous stirring is required to ensure sufficient contact between the enzyme and the sheep liver to improve the enzymatic hydrolysis efficiency.
[0061] S3. Enzyme inactivation: After the enzymatic hydrolysis reaction is completed, the hydrolyzate is quickly heated to 90°C and maintained for 10 minutes to inactivate the enzyme and terminate the enzymatic hydrolysis reaction. The purpose of this step is to prevent the enzyme from continuing to act, resulting in excessive decomposition of the peptide fragments and affecting the quality and activity of the product.
[0062] S4. Separation and purification: Use centrifugation and filtration to remove the residue and insoluble impurities in the enzymatic hydrolyzate to obtain a clarified sheep liver peptide solution; use ultrafiltration and chromatography technology to separate and purify the sheep liver peptide solution to remove salt, small molecule impurities and large molecule proteins to obtain a purified sheep liver peptide solution.
[0063] S5. Concentration and drying: Concentrate the purified sheep liver peptide solution to remove water and increase the concentration of the sheep liver peptide.
[0064] S6. Drying and powdering: Use spray drying or freeze drying to dry the concentrated sheep liver peptide solution into a powdered product.
[0065] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the present invention to specific embodiments. Obviously, many modifications and variations are possible based on the contents of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.
Claims
1. The nutritional formula of sheep liver peptide based on low-temperature enzymatic hydrolysis technology is characterized by: The main component of the sheep liver peptide nutritional formula is sheep liver peptide, which accounts for 50-70% by weight and is extracted by low-temperature enzymatic hydrolysis technology; The antioxidant system of this sheep liver peptide nutritional formula includes vitamin C and vitamin E; The trace elements of this sheep liver peptide nutritional formula include zinc and selenium.
2. The sheep liver peptide nutritional formula according to claim 1, characterized in that: The added amount of the vitamin C is 200-500 mg / 100 g of sheep liver peptide.
3. The sheep liver peptide nutritional formula according to claim 1, characterized in that: The added amount of the vitamin E is 50-150 mg / 100 g of sheep liver peptide.
4. The sheep liver peptide nutritional formula according to claim 1, characterized in that: The added amount of zinc is 5-15 mg / 100 g of sheep liver peptide.
5. The sheep liver peptide nutritional formula according to claim 1, characterized in that: The added amount of selenium is 20-50 μg / 100g of sheep liver peptide.
6. The efficient extraction process of sheep liver peptide based on low temperature enzymatic hydrolysis technology is characterized by: The steps include: S1. Raw material pretreatment: Select fresh, high-quality sheep liver as raw material, wash the liver, remove the connective tissue and impurities on the surface, and then cut it into small pieces for subsequent processing; S2. Low-temperature enzymatic hydrolysis: Add an appropriate amount of purified water to the pretreated sheep liver block and stir evenly to prepare a liver tissue homogenate; adjust the pH value of the homogenate to 6.5-7.5, then add the enzyme preparation and perform enzymatic hydrolysis at a low temperature of 30-40°C for 4-8 hours; S3, enzyme inactivation treatment: After the enzymatic hydrolysis reaction is completed, the enzymatic hydrolysis solution is quickly heated to 80-90°C and maintained for 10-15 minutes to inactivate the enzyme and terminate the enzymatic hydrolysis reaction; S4, separation and purification: using centrifugation and filtration to remove residues and insoluble impurities in the enzymatic hydrolyzate to obtain a clarified sheep liver peptide solution; using ultrafiltration and chromatography technology to separate and purify the sheep liver peptide solution to remove salt, small molecule impurities and large molecule proteins to obtain a purified sheep liver peptide solution; S5, concentration and drying: concentrating the purified sheep liver peptide solution to remove water therein and increase the concentration of the sheep liver peptide; S6. Drying and powdering: Use spray drying or freeze drying to dry the concentrated sheep liver peptide solution into a powdered product.
7. The efficient extraction process of sheep liver peptide according to claim 6, characterized in that: In step S2, the enzyme preparation is papain or trypsin.
8. The efficient extraction process of sheep liver peptide according to claim 6, characterized in that: In step S2, continuous stirring is required during the enzymatic hydrolysis process to ensure that the enzyme is fully in contact with the sheep liver and to improve the enzymatic hydrolysis efficiency.