EGCG (epigallocatechin gallate)-containing brightening and anti-aging composition as well as preparation method and application thereof

Abstract

The invention discloses a brightening and anti-aging composition containing EGCG (epigallocatechin gallate) as well as a preparation method and application of the brightening and anti-aging composition, and relates to the technical field of skin products. The invention provides a brightening and anti-aging composition containing EGCG (epigallocatechin gallate). The brightening and anti-aging composition containing EGCG is prepared from the following components in parts by weight: 0.5 to 2 parts of an epigallocatechin gallate nano composition, 0.05 to 0.5 part of glutamine and 0.05 to 0.5 part of undecylenoyl phenylalanine. According to the epigallocatechin gallate nano composition disclosed by the invention, the stability of a formula can be improved and the problem of oxidation yellowing can be improved when the three components of the epigallocatechin gallate nano composition, the glutathione and the undecylenoyl phenylalanine are cooperatively used; meanwhile, the three components all belong to micromolecular raw materials, can rapidly permeate into the skin, can effectively brighten the skin color, resist skin aging and comprehensively repair damaged skin, and are high in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of skin products, in particular to an EGCG-containing brightening and anti-aging composition and a preparation method and application thereof. Background Art

[0002] As people's living standards continue to improve, the demand for skin care is growing. Skin brightening, anti-aging, and repair have become a focus of attention in the skincare field. Traditional whitening products for brightening skin often use ingredients such as hydroquinone and kojic acid. However, hydroquinone has certain cytotoxic properties, posing potential risks to the human body with long-term use, and its use has been strictly restricted in many countries. Kojic acid, on the other hand, suffers from poor stability and is prone to decomposition during storage and use, significantly reducing its whitening effect. Regarding anti-aging, while common retinoids can promote collagen production and have anti-aging effects, they can also cause skin sensitivity, leading to adverse reactions such as redness, swelling, and scaling, limiting their widespread use. Furthermore, many anti-aging products only address a single aspect of skin aging, failing to comprehensively address the multiple issues of skin aging, resulting in unsatisfactory anti-aging results. Regarding skin repair, existing products primarily focus on alleviating surface symptoms, lacking effective means for deeper repair of damaged cells and restoration of the skin's barrier function. Some repair products contain hormones, which can also lead to dependence, causing serious problems such as steroid-induced dermatitis.

[0003] As can be seen, current skincare products on the market struggle to achieve synergistic benefits in brightening, anti-aging, and repairing skin, resulting in poor results and safety. Therefore, developing a safe and effective skincare composition that effectively brightens skin tone, combats aging, and comprehensively repairs damaged skin has become a critical issue in this field. Summary of the Invention

[0004] Based on this, the purpose of this application is to overcome the shortcomings of the above-mentioned prior art and provide a brightening and anti-aging composition that can effectively brighten the skin tone, resist skin aging, and comprehensively repair damaged skin, and is highly safe, as well as its preparation method and application.

[0005] To achieve the above objectives, in the first aspect of the present application, the technical solution adopted by the present application is: a brightening and anti-aging composition containing EGCG, comprising the following components in parts by weight: 0.5-2 parts of epigallocatechin gallate nanocomposition, 0.05-0.5 parts of glutathione, and 0.05-0.5 parts of undecylenoyl phenylalanine.

[0006] EGCG, the most potent antioxidant in tea, contains eight phenolic hydroxyl groups in its molecular structure. This structure gives it potent biological activity, but also results in its limited stability. Despite this, EGCG demonstrates significant anti-inflammatory and skin-repairing effects. Its anti-inflammatory properties can inhibit the release of inflammatory mediators, modulate inflammatory signaling pathways, and effectively combat oxidative stress, leading to its widespread use in health supplements and pharmaceuticals. In the area of skin repair, EGCG can promote the proliferation and differentiation of skin cells, accelerating the renewal of damaged cells. Specifically, it stimulates fibroblast activity, promotes the synthesis of collagen and elastin fibers, enhances skin toughness and elasticity, and significantly repairs structural damage caused by aging and UV damage. Glutathione is a key antioxidant within human cells, actively participating in cellular redox reactions and maintaining intracellular redox balance. On the one hand, it enhances the antioxidant capacity of skin cells, reduces free radical damage, and thus slows skin aging. On the other hand, glutathione inhibits tyrosinase activity, reducing melanin production through synthesis, achieving whitening and spot reduction. It also binds to existing melanin, accelerating its breakdown and metabolism. Undecylenoylphenylalanine has significant advantages in the skin whitening field. It inhibits the binding of α-melanocyte-stimulating hormone (α-MSH) to the melanocyte surface receptor MC1R, blocking melanocyte activation signals at the source and precisely reducing melanin synthesis. It significantly reduces dark spots caused by factors such as UV exposure and endocrine disorders. This ingredient is mild and hypoallergenic, with minimal skin irritation and low allergenicity, making it suitable for most skin types, including sensitive skin. While this ingredient can inhibit melanin at the source, its inhibitory effect on already produced melanin is weak, so it needs to be combined with other ingredients to achieve a long-lasting whitening effect within the skin, leaving it fairer and more translucent.

[0007] The three components of the present invention, epigallocatechin gallate nanocomposite, glutathione and undecylenoylphenylalanine, when used synergistically, can improve the stability of the formula and alleviate the problem of oxidative yellowing. At the same time, the above three components are all small molecule raw materials that can quickly penetrate the skin, improving the use effect while achieving long-lasting and stable efficacy.

[0008] During the actual experimental process, the inventors found that when the three components of epigallocatechin gallate nanocomposite, glutathione and undecylenoylphenylalanine are used synergistically: in terms of brightening skin color, the three respectively act on the inflammatory triggering end of melanin production - EGCG, the enzyme catalytic end - glutathione - and the signal transduction end - undecylenoylphenylalanine, achieving full pathway coverage from "signal blocking-enzyme activity inhibition-pigment reduction"; in terms of anti-aging, EGCG dominates collagen synthesis and protection, glutathione maintains the youthful redox state of cells, and undecylenoylphenylalanine blocks aging-related pigment transmission. The three form an anti-aging closed loop at the levels of dermis (structural support), epidermis (barrier function) and intercellular communication (signal regulation); in terms of barrier repair, EGCG quickly fights inflammation and maintains stability, glutathione deeply repairs oxidative damage, and undecylenoylphenylalanine blocks the inflammation-barrier destruction cycle. The three form a time gradient synergy in acute inflammation control and long-term barrier reconstruction. When the three components are used in conjunction, a brightening and anti-aging composition is provided that can effectively brighten the skin tone, resist skin aging, and comprehensively repair damaged skin with high safety.

[0009] The epigallocatechin gallate nanocomposition of the present invention can be a commercially available product or homemade. The preparation method has a reference application number: 202410920897.3. The invention name is: An EGCG nanocomposition with strong antioxidant and anti-photoaging effects, and its preparation method and application.

[0010] Specifically, it is achieved through the following technical solution: the EGCG nanocomposite is formed by two-step functional phospholipid self-assembly to encapsulate the active ingredient EGCG, including the first step of nanocomposite precursor preparation process and the second step of dual phospholipid nanocomposite preparation process.

[0011] A method for preparing an EGCG nanocomposition with strong antioxidant and anti-photoaging effects comprises the following steps:

[0012] (1) Preparation of a nanocomposite precursor: adding phospholipids and EGCG to an organic solvent and stirring uniformly to obtain an organic phase; adding a polyol to water and stirring uniformly to obtain an aqueous phase; dropping the organic phase into the aqueous phase at a uniform rate, and continuing to stir after the addition is complete to obtain a mixed solution; removing the organic solvent from the obtained mixed solution; and drying the mixed solution to obtain a nanocomposite precursor; the phospholipid content in the mixed solution is 1% to 10% by mass;

[0013] (2) Preparation of dual phospholipid nanocomposites:

[0014] The phospholipid, rigid membrane material stabilizer, and flexible membrane material stabilizer are added to a polyol and stirred to obtain an alcohol phase; the nanocomposite precursor is added to water and stirred to obtain an aqueous phase; the aqueous phase is uniformly added dropwise to the alcohol phase, and after the addition is complete, stirring is continued to obtain a mixed solution; the mixed solution is subjected to nano-processing to obtain an EGCG nanocomposite, wherein the mass percentage of EGCG in the EGCG nanocomposite is 0.6%-6.0%;

[0015] In terms of mass percentage, the phospholipid content in the mixed solution is 0.1%-1.0%, the rigid membrane material stabilizer content is 0.1%-0.3%, and the flexible membrane material stabilizer content is 0.5%-2.0%;

[0016] The molecular structure of the rigid film material stabilizer contains polycyclic structural units or benzene ring structural units; the molecular structure of the flexible film material stabilizer is a straight chain type or a straight chain compound.

[0017] Preferably, the EGCG-containing brightening and anti-aging composition comprises the following components in parts by weight: 1-1.5 parts of epigallocatechin gallate nanocomposition, 0.1-0.3 parts of glutathione, and 0.1-0.3 parts of undecylenoylphenylalanine.

[0018] The inventors found in actual experiments that when the weight proportions of the components in the brightening and anti-aging composition are further selected to be within the above ranges, the overall performance of the obtained product is better.

[0019] Preferably, based on the total weight of the brightening and anti-aging composition containing EGCG, the weight percentage of the epigallocatechin gallate nanocomposition is 70-80%.

[0020] Preferably, the weight percentage of glutathione is 10-20% based on the total weight of the brightening and anti-aging composition.

[0021] During actual experiments, the inventors found that further limiting the mass percentage range of the epigallocatechin gallate nanocomposition or glutamic acid in the total mass of the brightening and anti-aging composition can achieve better results. The resulting product is more effective in brightening the skin tone, resisting skin aging, and comprehensively repairing damaged skin.

[0022] In the second aspect of the present application, the present application provides the use of the EGCG-containing brightening and anti-aging composition in the preparation of skin products.

[0023] In a third aspect of the present application, the present application provides a skin product comprising the EGCG-containing brightening and anti-aging composition.

[0024] Preferably, the skin products include cosmetics and skin care products; the skin care products include any one of lotion, emulsion, cream, mask, essence, essence oil, spray, and sunscreen; the cosmetics include any one of liquid foundation and lipstick.

[0025] Preferably, the skin product is a facial cream.

[0026] In a fourth aspect of the present application, a facial cream is provided, comprising the following components in percentage by mass: 0.5-3% of the EGCG-containing brightening and anti-aging composition, 2-40% of a skin product base, and the balance being water.

[0027] Preferably, the skin product matrix includes at least one of a thickener, a moisturizer (including oils), an emulsifier, a skin conditioner, a preservative, a fragrance, and a pH adjuster.

[0028] Preferably, the thickener includes at least one of hydroxyethyl cellulose, carbomer, xanthan gum, guar gum, sodium carboxymethyl cellulose, polyvinyl alcohol, etc.; the moisturizer includes at least one of glycerin, hyaluronic acid, butylene glycol, propylene glycol, sorbitol, sodium hyaluronate, polyethylene glycol, xylitol, jojoba oil, olive oil, almond oil, rosehip oil, grape seed oil, white meadowfoam seed oil, grape seed oil, sunflower seed oil, avocado tree butter, vaseline, liquid paraffin, etc.; the emulsifier includes at least one of fatty acid glycerides, polysorbates, spans, Tweens, lecithin, glyceryl stearate, polyoxyethylene ether, etc.; the skin conditioner includes at least one of allantoin, squalane, etc.; the preservative includes at least one of parahydroxybenzoates, phenoxyethanol, potassium sorbate, sodium benzoate, sodium dehydroacetate, propylparaben, chlorphenesin, etc.; the pH adjuster includes at least one of triethanolamine, citric acid, sodium hydroxide, etc.

[0029] Compared with the prior art, the beneficial effects of the present invention are as follows: when the three components of epigallocatechin gallate nanocomposite, glutathione and undecylenoylphenylalanine are used synergistically: in terms of brightening skin color, the three respectively act on the inflammatory triggering end of melanin production - EGCG, the enzyme catalytic end - glutathione - and the signal transduction end - undecylenoylphenylalanine, achieving full pathway coverage from "signal blocking-enzyme activity inhibition-pigment reduction"; in terms of anti-aging, EGCG leads collagen synthesis and protection, glutathione maintains the youthful redox state of cells, and undecylenoylphenylalanine blocks aging-related pigment transmission. The three form an anti-aging closed loop at the levels of dermis (structural support), epidermis (barrier function) and intercellular communication (signal regulation); in terms of barrier repair, EGCG quickly fights inflammation and maintains stability, glutathione deeply repairs oxidative damage, and undecylenoylphenylalanine blocks the inflammation-barrier destruction cycle. The three form a time gradient synergy in acute inflammation control and long-term barrier reconstruction. When the three components are used in conjunction, a brightening and anti-aging composition is provided that can effectively brighten the skin tone, resist skin aging, and comprehensively repair damaged skin with high safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 Brighten the test image for the zebrafish. DETAILED DESCRIPTION

[0031] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings and specific examples. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0032] The raw materials used in the examples of this application are now further described, but are not limited to the following raw materials:

[0033] Epigallocatechin gallate nanocomposite, glutathione, and undecylenoylphenylalanine are all sourced from Guangzhou Xiyuan Biotechnology Co., Ltd.

[0034] The EGCG nanocomposition-1 is from Guangzhou Xiyuan Biotechnology Co., Ltd., and the active ingredient EGCG accounts for 3% of the EGCG nanocomposition.

[0035] EGCG nanocomposite-2 was prepared in-house. The preparation method is as follows. The EGCG used (purity 98%) can be purchased directly from Shanghai Aladdin Biotechnology Co., Ltd.:

[0036] (1) Preparation of nanocomposite precursor: 5.0% by mass of soybean lecithin and 15% by mass of EGCG were added to 30% by mass of anhydrous ethanol, and stirred at 60°C (speed of 300 rpm) for 30 minutes until the above raw materials were fully dissolved to obtain an organic phase. 5% by mass of maltitol was added to 45% by mass of deionized water, and stirred at 60°C (speed of 300 rpm) for 30 minutes until the above raw materials were fully dissolved to obtain an aqueous phase. Under the condition of maintaining the above organic phase, the above organic phase was uniformly added dropwise to the aqueous phase at a drop rate of 3 ml / min. After the dropwise addition was completed, the mixture was stirred at 60°C (speed of 300 rpm) for 15 minutes to obtain a mixed solution. The obtained mixed solution was subjected to a rotary evaporation operation to remove anhydrous ethanol, with a rotary evaporation speed of 150 rpm, a rotary evaporation temperature of 40°C, and a rotary evaporation time of 2 hours. The mixture was then freeze-dried for 48 hours to obtain a nanocomposite precursor. In the nanocomposite precursor, calculated by mass percentage, phospholipid: EGCG: maltitol = 20%: 60%: 20%.

[0037] (2) Preparation of EGCG nanocomposite: 0.3% by mass of hydrogenated lecithin, 0.15% by mass of cholesterol, and 1.5% by mass of caprylic / capric triglyceride were added to a mixed solution of 25% by mass of glycerol and 10% by mass of 1,2-hexanediol. The mixture was stirred at 30°C (300 rpm) for 30 min until the above raw materials were fully dissolved to obtain an alcohol phase. 5% by mass of the nanocomposite precursor was added to 58.05% by mass of deionized water. The mixture was stirred at 30°C (300 rpm) for 30 min until the nanocomposite precursor was uniformly dispersed to obtain an aqueous phase. While maintaining the temperature at 30°C, the aqueous phase was uniformly added dropwise to the alcohol phase at a rate of 3 ml / min. After the addition was completed, the mixture was stirred at 30°C (300 rpm) for 15 min to obtain a mixed solution. The mixed solution was dispersed by high-speed shearing (10000 rpm) for 20 min; and then subjected to high-pressure microfluidization homogenization with a homogenization pressure of 80 MPa and four homogenization times to obtain an EGCG nanocomposite.

[0038] The active ingredient EGCG accounts for 3% of the EGCG nanocomposition.

[0039] Tea polyphenols: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

[0040] Nonapeptide-1: purchased from Zhejiang Paipeptide Biological Co., Ltd.

[0041] Tranexamic acid: purchased from Wuhan Haishan Technology Co., Ltd.

[0042] The rest of the raw materials in the skin care product formula are conventional commercially available products.

[0043] Examples and Comparative Examples

[0044] The present invention provides a brightening and anti-aging composition containing EGCG. The components and weight portions of the brightening and anti-aging composition containing EGCG are shown in Table 1-2. The preparation method of the brightening and anti-aging composition containing EGCG comprises the following steps: uniformly mixing the components to obtain the brightening and anti-aging composition containing EGCG.

[0045] Table 1

[0046]

[0047] Table 2

[0048]

[0049]

[0050] Performance Test-1 Zebrafish Skin Brightening Effect Test

[0051] Test groups: compositions prepared in Examples 1-6 and Comparative Examples 1-9.

[0052] Methods sourced from the "Standard Operating Procedure for the Evaluation of Skin Brightening Effects in Zebrafish"; System and Sample Size: Experimental system: Zebrafish (Albino) with a melanin allele mutation. Zebrafish age: 2 days post-fertilization (dpf). Sample size per group: 15 fish. Adult fish husbandry and breeding methods: These methods were performed in accordance with our laboratory's standard husbandry and breeding methods, in compliance with AAALAC accreditation (certification number: 001458).

[0053] Principle and Method: Dull skin tone is the result of multiple internal and external factors, a key factor being oxidative stress caused by reactive oxygen species (ROS). Menadione, a benzoquinone compound, generates ROS. Oxidative stress occurs when the amount of free radicals produced exceeds the body's ability to clear them. Menadione is used to induce skin tone and then added to the sample. This antioxidant mechanism improves skin brightness by donating electrons, inhibiting oxidation, or by absorbing and scavenging free radicals, ultimately brightening the complexion.

[0054] Experimental steps: 1. Randomly select zebrafish in a 6-well plate, with 15 in each well. 2. Give menadione to establish a zebrafish pigmentation model. 3. Give the compositions of Examples 1-6 and Comparative Examples 1-9 diluted to a concentration of 0.1% each in water, and set up a normal control group (0.1% vitamin C) and a model control group (pure water) at the same time, with a capacity of 3 mL per well. 4. Incubate in the dark at 28°C for 22 hours. 5. Randomly select 10 zebrafish from each experimental group and place them under a dissecting microscope to take pictures. Use advanced image processing software to analyze and collect data, analyze the zebrafish yolk sac pigment signal intensity (S), and calculate and determine whether the sample has the effect of brightening the skin color according to the formula.

[0055] Skin lightening effect (%) = [S (model control group) - S (sample group)] / [S (model control group) - S (normal control group)] × 100%;

[0056] Applicability and Limitations: This test is suitable for testing the skin-lightening effects of skin products and their ingredients. The sample must be soluble in water or prepared into a suspension that can be evenly dispersed in water. Criteria: A statistically significant difference is considered significant if p < 0.05.

[0057] Test results: as shown in Table 3.

[0058] Table 3 Results of the skin lightening effect test of the samples

[0059]

[0060]

[0061] As can be seen from the above table, the composition prepared in the embodiment of the present invention, when the three components of the EGCG nanocomposite, glutathione and undecylenoylphenylalanine are used in conjunction, has a good effect in brightening the skin tone. Figure 1 These are microscope photos of zebrafish in the normal control group, model control group, and example. It can be seen that the composition prepared in the example of the present invention has a good effect in brightening the skin tone.

[0062] As can be seen from Example 1 and Comparative Examples 1-5, when one of the components is not added, or when it is not added and the other components are used to make up the difference, the effects of the present invention cannot be achieved; as can be seen from Example 1 and Comparative Examples 6-8, when other similar components are used instead, the corresponding effects of the present invention cannot be achieved. As can be seen from Example 1 and Comparative Example 9, when the weight ratios of the components in the composition are not within the range of this application, a good synergistic effect cannot be achieved.

[0063] Application Examples

[0064] The application examples, comparative application examples and blank application examples of the present invention provide a facial cream, the components (mass percentage) of the facial cream are shown in Table 4; wherein, the EGCG-containing brightening and anti-aging compositions used in Application Examples 1-6 are respectively the EGCG-containing brightening and anti-aging compositions prepared in Examples 1-6, for example, the brightening and anti-aging composition containing EGCG in Example 1 is used in Application Example 1, the brightening and anti-aging composition containing EGCG in Example 2 is used in Application Example 2, and so on; the compositions used in Comparative Application Examples 1-9 are respectively the compositions prepared in Comparative Examples 1-9; the composition used in Application Example 7 is the composition prepared in Example 1;

[0065] Table 4

[0066]

[0067]

[0068] The preparation method of the facial cream in Application Example 1 comprises the following steps:

[0069] Preparation: Accurately weigh all ingredients from the above formula and prepare clean mixing equipment, heating equipment (such as a constant temperature water bath), emulsifying equipment (such as a homogenizer), and storage containers. Ensure the operating environment is clean and tidy to avoid impurities and microbial contamination.

[0070] Preparation of oil phase: Add meadowfoam seed oil, grape seed oil, sunflower seed oil, avocado butter, cetearyl olivate and sorbitan olivate to a clean container, place in a constant temperature water bath, heat to 75°C, and continue stirring until all ingredients are completely melted and evenly mixed to form an oil phase.

[0071] Preparation of aqueous phase: In another clean container, add xylitol glucoside, dehydrated xylitol, brightening and anti-aging composition, acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer and deionized water in sequence, stir well, and heat to 75°C to ensure that all ingredients are completely dissolved to form an aqueous phase.

[0072] Emulsification: While stirring continuously, slowly add the water phase to the oil phase, stirring constantly. Then, use a homogenizer to emulsify the mixture thoroughly, blending the oil and water phases to form a smooth, even cream. The emulsification time is controlled within 10 minutes, which can be adjusted as needed.

[0073] Adjust the pH value: When the temperature of the cream drops to 55°C, slowly add sodium hydroxide while stirring. At the same time, use pH test paper or pH meter to monitor the pH value of the cream and adjust it to about 6.0 to be suitable for skin use.

[0074] Add fragrance and preservatives: When the temperature of the cream drops below 40°C, add phenoxyethanol and fragrance in turn, stir well, and make them evenly dispersed in the cream.

[0075] Packaging and Storage: Cool the prepared cream to room temperature, place it in a clean container, and seal it tightly. Avoid direct sunlight and high temperatures during storage to ensure the quality and stability of the cream.

[0076] Performance Test-2 Stability Test

[0077] Test process: Compositions prepared in Examples 1-6, and creams prepared in Application Examples 1-6 and Comparative Application Examples 1-3.

[0078] After all physical and chemical indicators and appearance indicators meet the standards, the samples are divided into two groups for testing:

[0079] (1) Normal temperature test group: placed at 25°C for 7 days and 45 days;

[0080] (2) Heat resistance test group, placed at 45°C for 7 days and 45 days;

[0081] The test samples were observed on the 7th and 45th days after treatment, respectively. The observation items were the appearance, color, and skin feel of the test samples.

[0082] The stability test time is generally 7 days, and the situation is summarized and checked every 7 days. If there is a special requirement to extend the test time, it will be carried out for 2 or 3 months. The above experimental plan needs to be compared with the product at room temperature to check the material condition and record the test results in the "Formula Stability Test Report". The test results are shown in Table 5.

[0083] Table 5 Stability test results of different compositions and their application in facial cream

[0084]

[0085]

[0086] As can be seen from the above table, the compositions prepared in the examples of the present invention and the use of the compositions prepared in the examples in facial creams can both meet the stability requirements.

[0087] Performance Test-3 Anti-wrinkle and Firming Efficacy Test - Human Fibroblast Type I Collagen (Collagen I) Immunofluorescence Detection

[0088] Collagen I is the main protein in the skin's extracellular matrix. It forms highly ordered collagen fibers, providing structural support and elasticity to the skin. Mature Collagen I can self-assemble into collagen fibers, which are key to skin firmness and elasticity. With age, the amount of Collagen I in the skin gradually decreases, causing the skin to lose elasticity and firmness, thus forming wrinkles. Therefore, by promoting the synthesis of Collagen I, the elasticity of the skin can be enhanced and the formation of wrinkles can be reduced. This test uses human fibroblasts as the test system. The changes in the expression of type I collagen in human fibroblasts are detected by immunofluorescence technology to evaluate whether the prepared product has anti-wrinkle and firming effects.

[0089] Cell Preparation: Cultivate human fibroblasts to an appropriate growth state, typically in a culture flask or dish. Digest the cells with trypsin, collect the cell pellet, and resuspend in culture medium to create a single-cell suspension. Count and adjust the cell concentration to an optimal state before seeding into a 6-well plate or other suitable culture container. Add a small amount of culture medium to the center of the well, then place a coverslip. Slowly add the cell suspension dropwise, gently mix, and culture in a CO2 incubator overnight to allow the cells to adhere and grow.

[0090] UVA radiation treatment: According to the experimental design, human fibroblasts grown on the wall were subjected to UVA radiation treatment. The human fibroblasts treated with UVA radiation were incubated with Examples 1-6, Comparative Examples 1-9, and NC group (ergothioneine), and the changes in the content of Collagen I were determined after 12 hours and 24 hours of treatment. The results of the 12-hour Collagen I content determination. The results of the 24-hour Collagen I content determination showed that regardless of whether the human fibroblasts treated with UVA radiation were treated for 12 hours or 24 hours, based on the UVA-stimulated human fibroblast model, when the sample concentration in the DMSO solution was 0.05% (V / V), the content of type I collagen (Collagen I) in human fibroblasts was significantly increased, and there was a statistical difference compared with the NC group (p < 0.01), indicating that the sample has anti-wrinkle and firming effects.

[0091] Test results: as shown in Table 6.

[0092] Table 6 Effect of different samples on the content of type Ⅰ collagen in human fibroblasts (%)

[0093] Case 0h collagen content / % 12h collagen content / % 24h collagen content / % Example 1 17 165 216 Example 2 17 132 198 Example 3 16 145 204 Example 4 18 126 176 Example 5 17 130 187 Example 6 18 163 215 Comparative Example 1 19 21 57 Comparative Example 2 16 24 58 Comparative Example 3 17 25 88 Comparative Example 4 19 28 102 Comparative Example 5 24 32 111 Comparative Example 6 17 67 132 Comparative Example 7 18 64 121 Comparative Example 8 25 54 118 Comparative Example 9 16 76 142 Positive control group 15 121 173

[0094] As can be seen from the above table, the composition prepared by the present invention, wherein the three components of the EGCG nanocomposite, glutathione and undecylenoylphenylalanine are used in synergistic manner, has a very good effect in terms of anti-wrinkle and firming.

[0095] As can be seen from the comparison between Example 1 and Comparative Examples 1-5, the effects of the present invention cannot be achieved when one or two of the components are not added, or when they are not added and the other components are used to make up for the difference. As can be seen from the comparison between Application Example 1 and Comparative Application Examples 6-8, the effects of the present invention cannot be achieved when other similar components are substituted. As can be seen from the comparison between Example 1 and Comparative Example 9, when the weight percentages of the components in the composition are not within the range of this application, a good synergistic effect cannot be achieved.

[0096] Performance Test-4 Human Body Repair Barrier Test

[0097] 1. Test samples: facial creams prepared in Application Examples 1-7, blank application examples, and comparative application examples 1-9.

[0098] 2. Subjects: There were 17 groups of 5 people in each group, with an average age of 35.44±5.42 years old, ranging from 25 to 58 years old, meeting the voluntary inclusion criteria.

[0099] 3. Test instruments:

[0100] Skin moisture loss tester TM 300 (Courage+Khazaka, Germany)

[0101] 4. Principle of barrier repair effect: Use Tewameter to test 3 times and take the average value.

[0102] (1) Parameter explanation: Based on Fick's diffusion law, the built-in capacitive sensor detects the water vapor pressure formed on the skin surface and calculates the transepidermal water loss (TEWL). The smaller the TEWL value, the better the skin barrier.

[0103] (2) Result determination: After using the product, the TEWL value of the test area was significantly reduced, indicating that the test product has the effect of repairing the skin barrier.

[0104] 5. The test method for skin barrier repair efficacy is as follows:

[0105] This single-center open trial involved subjects using the product for 4 weeks, with self-control before and after use. Instrumental testing was used to measure the transepidermal water loss rate before and after 4 weeks of use to verify the product's effectiveness in repairing the skin barrier.

[0106] Before using the product, test the subject's health with the instrument and record their results, which is recorded as W0. Use the product daily, morning and evening, for four consecutive weeks. Then, test the subject's health with the instrument again and record their results, which is recorded as W4. The formula for calculating the change rate is: (W4 - W0) / W0 = Change Rate (%).

[0107] Table 7 Transepidermal water loss rate (%)

[0108]

[0109]

[0110] As can be seen from Table 7 above, the cream prepared in the application example of the present invention has a good effect on repairing the barrier when the three components of the EGCG nanocomposite, glutathione and undecylenoylphenylalanine are used in coordination.

[0111] As can be seen from the comparison between Application Example 1 and Comparative Application Examples 1-5, the effects of the present invention cannot be achieved when one or two of the components are not added, or when they are not added and the proportions are supplemented with other components. As can be seen from the comparison between Application Example 1 and Comparative Application Examples 6-8, the effects of the present invention cannot be achieved when other similar components are substituted. As can be seen from the comparison between Application Example 1 and Comparative Application Example 9, when the weight proportions of the components in the composition are not within the range of this application, a good synergistic effect cannot be achieved.

[0112] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A brightening and anti-aging composition containing EGCG, characterized in that: The invention comprises the following components in parts by weight: 0.5-2 parts of epigallocatechin gallate nanocomposite, 0.05-0.5 parts of glutathione, and 0.05-0.5 parts of undecylenoylphenylalanine.

2. The EGCG-containing brightening and anti-aging composition according to claim 1, wherein The invention comprises the following components in parts by weight: 1-1.5 parts of epigallocatechin gallate nanocomposition, 0.1-0.3 parts of glutathione, and 0.1-0.3 parts of undecylenoylphenylalanine.

3. The brightening and anti-aging composition according to claim 1, wherein Based on the total weight of the brightening and anti-aging composition containing EGCG, the weight percentage of the epigallocatechin gallate nanocomposition is 70-80%.

4. The brightening and anti-aging composition according to claim 1, wherein Based on the total weight of the brightening and anti-aging composition containing EGCG, the weight percentage of glutathione is 10-20%.

5. Use of the EGCG-containing brightening and anti-aging composition according to any one of claims 1 to 4 in the preparation of skin products.

6. A skin care product, characterized in that: The invention comprises the EGCG-containing brightening and anti-aging composition according to any one of claims 1 to 4.

7. The skin care product according to claim 6, wherein: The skin products include cosmetics and skin care products; the skin care products include any one of lotion, emulsion, cream, mask, essence, essence oil, spray, and sunscreen; the cosmetics include any one of liquid foundation and lipstick.

8. The skin care product according to claim 7, wherein: The skin product is a facial cream.

9. A facial cream, characterized in that The facial cream comprises the following components in percentage by mass: 0.5-3% of the EGCG-containing brightening and anti-aging composition according to any one of claims 1 to 4, 2-40% of a skin product base, and the balance being water.

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