Dandruff removing composition, preparation method thereof and nursing product

Abstract

The invention relates to the technical field of cosmetics, in particular to a dandruff removing composition, a preparation method thereof and a nursing material. The anti-dandruff composition is prepared from the following components in percentage by weight: 0.1 to 5 percent of Mexican juniper oil, 0.5 to 8 percent of bitter oil tree seed oil, 1 to 10 percent of hydrogenated lecithin, 0.2 to 5 percent of phytosterol, 1 to 10 percent of natural emulsifier, 5 to 20 percent of natural vegetable fat, 0.2 to 5 percent of xanthan gum and the balance of water. According to the anti-dandruff cream, the bitter oil tree seed oil and the Mexican juniper oil are used as active ingredients, the stability and effectiveness of active matters are improved by utilizing a liposome technology, the skin permeability and the uniformity on scalp are improved, a better anti-dandruff effect is achieved, the safety is high, and skin irritation and eye irritation are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-dandruff composition, a preparation method thereof, and care products. Background Art

[0002] Due to stress, hormone regulation, and metabolism, abnormal dandruff growth is a serious problem, and shampoo products with anti-dandruff and anti-itching properties are widely used. Traditional anti-dandruff shampoos contain chemically synthesized ingredients, such as zinc pyrithione (ZPT), pyridone ethanolamine (OCT), hexamidine dihydroxyethylsulfonate, selenium disulfide, and salicylic acid. These ingredients not only fail to align with current natural and organic product concepts, but also present varying degrees of issues with product appearance, wash feel, and skin and eye irritation. Therefore, the market for naturally derived anti-dandruff actives is vast.

[0003] Traditional natural anti-dandruff ingredients include Platycladus orientalis extract, Sophora flavescens extract, Magnolia officinalis bark extract and other plant extracts with antibacterial and anti-inflammatory activities. In hair care products, anti-dandruff active ingredients are usually directly added to the formula. However, hair care products are usually rinse-off types, and their action time on the scalp and hair is short. When applied to the scalp, the distribution is not uniform, and the efficacy cannot be fully exerted. Therefore, the stability and effectiveness of natural anti-dandruff active ingredients in products need to be improved urgently. Summary of the Invention

[0004] Based on this, it is necessary to provide an anti-dandruff composition and a preparation method and care products thereof that can improve the stability of anti-dandruff active ingredients in shampoo.

[0005] In a first aspect, the present application provides an anti-dandruff composition, which is composed of the following components in percentage by weight:

[0006] 0.1-5% of Mexican juniper oil, 0.5-8% of tartar seed oil, 1-10% of hydrogenated lecithin, 0.2-5% of phytosterol, 1-10% of natural emulsifier, 5-20% of natural plant oil, 0.2-5% of xanthan gum and the balance of water.

[0007] Preferably, the composition consists of the following components in percentage by weight:

[0008] 0.1-2% of Mexican juniper oil, 0.5-5% of calendula seed oil, 2-5% of hydrogenated lecithin, 0.2-2% of phytosterol, 1-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-3% of xanthan gum and the balance of water.

[0009] Preferably, the composition consists of the following components in percentage by weight:

[0010] 0.2-1% of Mexican juniper oil, 0.8-4% of tartar seed oil, 2-4.5% of hydrogenated lecithin, 0.5-2% of phytosterol, 1.5-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

[0011] Preferably, the composition consists of the following components in percentage by weight:

[0012] 0.2-1% of Mexican juniper oil, 0.8-2% of tartar seed oil, 2-4.5% of hydrogenated lecithin, 0.5-1.5% of phytosterol, 1.5-6% of natural emulsifier, 5-18% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

[0013] Preferably, the natural emulsifier is composed of an emulsifying component and polyglyceryl-10 stearate, the weight ratio of the emulsifying component and polyglyceryl-10 stearate is (0.3-0.65):1, and the emulsifying component is composed of cetearyl alcohol and cetearyl glucoside; wherein the content of polyglyceryl-10 stearate in the anti-dandruff composition is greater than 0.6%.

[0014] Preferably, the natural plant oil is composed of one or more of sunflower seed oil, cobaiba oil, bacas seed oil, and sweet almond oil.

[0015] Preferably, the phytosterol consists of one or both of β-sitosterol and stigmasterol.

[0016] In a second aspect, the present application further provides a method for preparing the anti-dandruff composition, comprising the following steps:

[0017] S1: Mixing juniper oil and calendula seed oil to obtain a first intermediate; dissolving hydrogenated lecithin and phytosterol in a solvent to obtain a second intermediate;

[0018] S2: adding the first intermediate to the second intermediate, and removing the solvent to obtain a third intermediate;

[0019] S3: adding a buffer solution to the third intermediate, dispersing the mixture, and filtering the mixture with a microporous membrane to obtain liposomes containing juniper oil and calamagrostis seed oil;

[0020] S4: dissolving xanthan gum in an aqueous solvent and maintaining the solution at a first temperature; adding vegetable oil and natural emulsifier to the oil solvent, heating the solution to the first temperature until dissolved, and then adding the solution to the aqueous solvent and homogenizing the solution to obtain an emulsion;

[0021] S5: After the emulsion is cooled to below the second temperature, the juniper oil and calendula seed oil liposomes obtained in step S3 are added to obtain the anti-dandruff composition.

[0022] In a third aspect, the present application also provides a care product containing the anti-dandruff composition as described above.

[0023] Preferably, the care product is a shampoo product.

[0024] Beneficial effects: The present invention provides a natural anti-dandruff composition and a preparation method thereof, which have the following beneficial effects:

[0025] (1) The natural anti-dandruff composition prepared by the present invention uses tarragon seed oil and juniper oil as active ingredients for the first time, and uses liposome technology to improve the stability and effectiveness of the active ingredients, increase skin permeability and uniformity on the scalp, and achieve better anti-dandruff effect.

[0026] (2) The natural anti-dandruff composition prepared by the present invention has high stability, is easy to use, and effectively reduces the difficulty of the finished product process.

[0027] (3) The natural anti-dandruff composition prepared by the present invention is highly safe and has no skin irritation or eye irritation.

[0028] (4) The natural anti-dandruff composition prepared by the present invention is 100% natural and conforms to the current market product trend. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The following are the test results of the anti-dandruff performance of the shampoos prepared in some examples. DETAILED DESCRIPTION

[0030] To facilitate understanding of the present invention, the present invention will be described more fully below with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the accompanying drawings. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a more thorough and comprehensive understanding of the present disclosure.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0032] In a first aspect, the present application provides an anti-dandruff composition, which is composed of the following components in weight percentage: 0.1-5% of Mexican juniper oil, 0.5-8% of olea europaea seed oil, 1-10% of hydrogenated lecithin, 0.2-5% of phytosterol, 1-10% of natural emulsifier, 5-20% of natural plant oil, 0.2-5% of xanthan gum and the balance of water.

[0033] For example, juniper oil, whose main components are limonene and α-pinene, has strong antibacterial and astringent properties, is non-comedogenic, and has a fragrant aroma. It is often used as a disinfectant and calming product. The weight percentage of juniper oil can be, but is not limited to, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, or 5%.

[0034] For example, olea oleifera seed oil, also known as Andropa oil, is native to the Amazon basin in Brazil and has a golden color. It has anti-inflammatory, hair-strengthening, microbial-inhibiting, and mosquito-repellent properties. The weight percentage of olea oleifera seed oil may be, but is not limited to, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, or 8%.

[0035] Illustratively, the weight percentage content of hydrogenated lecithin can be, but is not limited to, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5% or 10%.

[0036] Illustratively, the weight percentage content of phytosterols can be, but is not limited to, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%.

[0037] Illustratively, the weight percentage content of the natural emulsifier can be, but is not limited to, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5% or 10%.

[0038] Illustratively, the weight percentage content of natural vegetable oil can be, but is not limited to, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 10.5%, 11%, 11.5%, 12%, 12.5%, 13%, 13.5%, 14%, 14.5%, 15%, 15.5%, 16%, 16.5%, 17%, 17.5%, 18%, 18.5%, 19%, 19.5% or 20%.

[0039] Illustratively, the weight percentage content of xanthan gum can be, but is not limited to, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5%.

[0040] The anti-dandruff composition is 100% naturally derived and has the efficacy of inhibiting Malassezia, removing dandruff, and alleviating itching. It utilizes the natural ingredients of calamagrostis seed oil and juniper oil as anti-dandruff active ingredients. Liposome technology is used to enhance the permeability and dispersibility of the active ingredients, increasing the effectiveness and stability of the natural plant-based anti-dandruff active ingredients. This allows the anti-dandruff ingredients to be applied to the scalp more evenly and promptly, thus enhancing the anti-dandruff effect without causing any skin irritation.

[0041] Preferably, the composition is composed of the following components in weight percentage: 0.1-2% of Mexican juniper oil, 0.5-5% of tamarind seed oil, 2-5% of hydrogenated lecithin, 0.2-2% of phytosterol, 1-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-3% of xanthan gum and the balance of water.

[0042] Preferably, the composition is composed of the following components in weight percentage: 0.2-1% of Mexican juniper oil, 0.8-4% of tamarind seed oil, 2-4.5% of hydrogenated lecithin, 0.5-2% of phytosterol, 1.5-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

[0043] Preferably, the composition is composed of the following components in weight percentage: 0.2-1% of Mexican juniper oil, 0.8-2% of tamarind seed oil, 2-4.5% of hydrogenated lecithin, 0.5-1.5% of phytosterol, 1.5-6% of natural emulsifier, 5-18% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

[0044] Preferably, the natural emulsifier is composed of an emulsifying component and polyglyceryl-10 stearate, the weight ratio of the emulsifying component to polyglyceryl-10 stearate being (0.3-0.65):1, and the emulsifying component is composed of cetearyl alcohol and cetearyl glucoside; wherein the content of polyglyceryl-10 stearate in the anti-dandruff composition is greater than 0.6%. Exemplarily, the emulsifying component can be a product sold by SEPPIC under the name Montanov, such as Montanov 68MB. In some embodiments, MONTANOV 68MB includes 75-85% cetearyl glucoside and 15-25% cetearyl alcohol.

[0045] Preferably, the natural vegetable oil is composed of one or more of sunflower seed oil, cobaiba oil, bacas seed oil, and sweet almond oil.

[0046] Preferably, the phytosterol consists of one or both of β-sitosterol and stigmasterol.

[0047] The present application also provides a method for preparing an anti-dandruff composition, comprising the following steps:

[0048] S1: Mixing juniper oil and calendula seed oil to obtain a first intermediate; dissolving hydrogenated lecithin and phytosterol in a solvent to obtain a second intermediate;

[0049] For example, Juniperus tataricus oil and Cercidiphyllum oleifera seed oil are mixed uniformly to obtain a first intermediate.

[0050] For example, hydrogenated lecithin and phytosterol are dissolved in an appropriate amount of anhydrous ethanol and dissolved in a water bath at 40°C.

[0051] S2: adding the first intermediate to the second intermediate, and removing the solvent to obtain the third intermediate.

[0052] Illustratively, after adding the first intermediate, the mixture is mixed evenly and the solvent is removed by rotary evaporation at 60°C.

[0053] S3: adding a buffer solution to the third intermediate, dispersing the mixture, and filtering the mixture with a microporous membrane to obtain liposomes containing juniper oil and calendula seed oil;

[0054] For example, PBS buffer (phosphate buffered saline) is added to the third intermediate obtained in S2, and after ultrasonic dispersion at a power of 150W, the material is filtered with a 0.22 μm microporous filter membrane to obtain liposomes of Mexican juniper oil and calendula seed oil.

[0055] S4: dissolving xanthan gum in an aqueous solvent and maintaining the solution at a first temperature; adding vegetable oil and natural emulsifier to the oil solvent, heating the solution to the first temperature until dissolved, and then adding the solution to the aqueous solvent and homogenizing the solution to obtain an emulsion;

[0056] For example, xanthan gum is completely dissolved in the water phase and kept warm at 75° C., vegetable oils and natural emulsifiers are added to the oil phase, heated at 75° C. until uniformly dissolved, and then added to the water phase and homogenized for 5 minutes to obtain an emulsion.

[0057] S5: After the emulsion is cooled to below the second temperature, the juniper oil and calendula seed oil liposomes obtained in step S3 are added to obtain an anti-dandruff composition.

[0058] Illustratively, the material is cooled to below 45° C., and the juniper oil and calendula seed oil liposomes prepared in S3 are added.

[0059] The present application also provides a care product containing the above anti-dandruff composition. Preferably, the care product is a shampoo product, such as shampoo.

[0060] The following are specific examples. The main experimental materials and their sources used in the examples are shown in Table 1 below.

[0061] Table 1 Main experimental materials and their sources

[0062]

[0063]

[0064] Example 1

[0065] In the examples and comparative examples of the present invention, the dosage of each component is shown in Table 2.

[0066] The preparation method of the natural anti-dandruff composition of this embodiment comprises the following steps:

[0067] S1: Mix juniper oil and calendula seed oil to obtain the first intermediate. Dissolve hydrogenated lecithin and phytosterol in an appropriate amount of anhydrous ethanol and dissolve in a water bath at 40°C to obtain the second intermediate.

[0068] S2: Add the first intermediate oil to the second intermediate, mix well, and remove the solvent by rotary evaporation at 60°C to obtain a third intermediate.

[0069] S3: PBS buffer was added to the third intermediate obtained in S2, and the mixture was ultrasonically dispersed at a power of 150 W, and then filtered through a 0.22 μm microporous filter membrane to obtain liposomes of Juniperus tataricus oil and Karasu seed oil.

[0070] S4: Dissolve xanthan gum completely in the water phase, keep warm at 75℃, add sunflower oil, sweet almond oil, cobaiba fat, bacas seed oil, emulsifier MONTANOV TM 68 and polyglycerol-10 stearate were added to the oil phase, heated at 75°C to dissolve evenly, then added to the water phase and homogenized at 10000 r / min for 2 min to obtain an emulsion.

[0071] S5: The material body is cooled to below 45° C., and the juniper tree oil and calendula seed oil liposomes prepared in S3 are added to obtain an anti-dandruff composition.

[0072] Example 2

[0073] The preparation method of Example 2 is substantially the same as that of Example 1, except for the content of each component in the anti-dandruff composition.

[0074] Example 3

[0075] The preparation method of Example 3 is substantially the same as that of Example 2, except for the content of each component in the anti-dandruff composition.

[0076] Example 4

[0077] The preparation method of Example 4 is substantially the same as that of Example 3, except that the amount of emulsifier polyglycerol-10 stearate added is reduced.

[0078] Example 5

[0079] The preparation method of Example 5 is substantially the same as that of Example 3, except that the amount of hydrogenated lecithin added is reduced.

[0080] Comparative Example 1

[0081] The preparation method of Comparative Example 1 is substantially the same as that of Example 3, except that the juniper tree oil and the calendula seed oil are not contained.

[0082] Comparative Example 2

[0083] The preparation method of Comparative Example 2 is substantially the same as that of Example 3, except that the anti-dandruff active ingredients, juniper oil and olea europaea seed oil, are replaced with a traditional anti-dandruff ingredient, pyridone ethanolamine salt (OCT).

[0084] Comparative Example 3

[0085] The preparation method of Comparative Example 3 is substantially the same as that of Example 3, except that the anti-dandruff active ingredient of this comparative example is not prepared in the form of liposomes, but is directly added to the composition.

[0086] The raw material parameters in the preparation methods of Examples 1 to 5 and Comparative Examples 1 to 3 are listed in Table 2 below:

[0087] Table 2 Raw material parameters of Examples and Comparative Examples

[0088]

[0089] The anti-dandruff compositions prepared in Examples 1 to 5 and Comparative Examples 1 to 3 were subjected to performance tests.

[0090] (1) Stability evaluation of natural anti-dandruff composition

[0091] The stability of the natural anti-dandruff composition was evaluated with reference to the method in T / SHFCA002-2021 "Guidelines for Cosmetic Stability Testing".

[0092] 1. Test items and operation steps

[0093] 1.1 Influencing factors test

[0094] 1.1.1 High temperature stability

[0095] Place the sample in a sealed clean container, then place it in a constant temperature incubator pre-adjusted to (45℃±2℃) for 30 days. Take samples on the 5th, 10th, 20th and 30th day. After the test, return to room temperature and observe whether the appearance, odor and pH of the sample are normal.

[0096] 1.1.2 Temperature stability

[0097] Place the sample in a sealed clean container, then place it in a constant temperature incubator pre-adjusted to (45℃±2℃), take it out after 24 hours, move it into a refrigerator pre-adjusted to (-5℃±2℃) and store it for 24 hours, then take it out, move it into a constant temperature incubator (40℃±2℃) and store it for 24 hours, then take it out again and place it into a refrigerator (-5℃±2℃) and store it for 24 hours. Repeat this cycle 5-7 times. After the test, return it to room temperature and observe whether the appearance, odor and pH of the sample are normal.

[0098] 1.2 Accelerated stability test

[0099] Place the sample in a constant temperature and humidity chamber at 40°C ± 2°C and 75% ± 5% relative humidity for 6 months. Test at least 4 time points (e.g., 1, 2, 3, and 6 minutes). Return to room temperature and compare with 0 minutes to observe whether the appearance, odor, and pH of the gas sample are normal.

[0100] 1.3 Long-term stability test

[0101] The samples were placed in a constant temperature and humidity chamber at 25°C ± 2°C and 60% ± 10% for 24 months. Samples were taken at 3, 6, 9, 12, 18, and 24 m, returned to room temperature, and compared with the temperature at 0 m. The appearance, odor, and pH of the gas samples were observed to ensure they were normal.

[0102] 2. Test results

[0103] The results of the stability evaluation test of the natural anti-dandruff composition are shown in Table 3.

[0104] Table 3 Stability evaluation test results of natural anti-dandruff composition

[0105] Sample name High temperature stability Temperature stability Acceleration stability Long-term stability Example 1 Stablize Stablize Stablize Stablize Example 2 Stablize Stablize Stablize Stablize Example 3 Stablize Stablize Stablize Stablize Example 4 Layering Stablize Layering Layering Example 5 Stablize Stablize Stablize Stablize Comparative Example 1 Stablize Stablize Stablize Stablize Comparative Example 2 Stablize Stablize Stablize Stablize Comparative Example 3 Stablize Stablize Stablize Stablize

[0106] The above results show that Examples 1, 2, 3, 5, and Comparative Examples 1-3 all showed no abnormalities in any of the stability evaluation tests, demonstrating excellent stability. Only the emulsion in Example 4 exhibited stratification at high temperatures, indicating poor stability. This is due to a reduced addition level of the emulsifier, polyglyceryl-10 stearate. Therefore, the stability evaluation experiments demonstrate that in this system, an addition level of polyglyceryl-10 stearate must be maintained above 0.6% to maintain stability.

[0107] (2) Evaluation of skin irritation of natural anti-dandruff composition

[0108] This test example is a skin occlusive patch test, which refers to the skin occlusive patch test method provided in the "Technical Specifications for Safety of Cosmetics" to test the irritation of the plant essential oil composition to human skin.

[0109] 1. Materials and Reagents

[0110] 1.1 Spot tester

[0111] This experiment uses the international standard Finn-Chamber, which is made of aluminum, has a diameter of 7 mm, and a sample volume of 15 μl.

[0112] 1.2 Test substance and concentration

[0113] Sodium lauryl sulfate is a classic skin irritation test substance. A 1% SLS aqueous solution was used as a positive control, and normal saline or distilled water was used as a negative control.

[0114] 1.3 Subjects

[0115] A total of 30 healthy subjects (15 females and 15 males, aged 20 to 48 years, with an average age of (27.07 ± 20.80) years) were enrolled. Subjects who had used glucocorticoids, nonsteroidal anti-inflammatory drugs, or antihistamines within the past 2 months were excluded. Furthermore, breastfeeding or pregnant women, those with skin diseases, sunburn, scarring, excessive tattoos, or genetic allergies were excluded. All subjects signed informed consent before the trial.

[0116] 2. Operation method

[0117] 2.1 Spot tester operation steps

[0118] Select the flexor side of the subject's upper arm as the enclosed patch test site, and use a pencil to mark the back of the patch tester in order from top to bottom and from left to right. Remove the coated paper on the patch tester, place the sample to be tested (15ul) in a small aluminum chamber, and apply it evenly. Apply the patch tester coated with chemical reagents to the patient's skin, stick it firmly from bottom to top, and press it gently with the palm of your hand to expel air. Remove the patch tester after 24 hours. To avoid the illusion caused by pressure from the patch tester, observe the skin reaction 30 minutes (after the indentation disappears), 24 hours, and 48 hours after removing the test sample patch tester, and record the observation results.

[0119] 2.2 Result evaluation method

[0120] The skin reaction is used to determine whether the test substance is an allergen. Visual scoring is performed according to five standard levels of 0-4. The meaning of the skin reaction represented by each grade score is as follows:

[0121] 0 points represent a negative reaction (-), which means no irritation to the skin;

[0122] 1 point represents a suspicious reaction (±), with only slight erythema on the skin;

[0123] 2 points represent a weak positive reaction (+, erythema reaction), with skin erythema, infiltration, edema, and papules;

[0124] 3 points represent a strong positive reaction (++, herpes reaction), with erythema, infiltration, edema, papules, and herpes appearing on the skin, and the reaction may extend beyond the tested area;

[0125] 4 points represent a very strong positive reaction (+++, confluent herpes reaction), with obvious erythema, severe infiltration, edema, and confluent herpes on the skin, and the reaction exceeds the tested area.

[0126] 2.3 Patch reagent samples

[0127] Examples 1-3 and Example 5 were used as samples for patch tests, and three parallel samples were set for each example sample.

[0128] 1% sodium lauryl sulfate was used as a positive control.

[0129] Physiological saline or distilled water was used as negative control.

[0130] 3. Test results

[0131] The results of the skin irritation test of the natural anti-dandruff composition using a spot tester are shown in Table 4.

[0132] All subjects had a positive control score of 4 and a negative control score of 0.

[0133] Table 4 Natural anti-dandruff composition skin irritation test results

[0134]

[0135]

[0136] It can be seen from the above results that all tested examples showed negative reactions, did not cause irritation to the skin, and had good safety performance when used in cosmetics.

[0137] (III) Evaluation of the performance of natural anti-dandruff composition in inhibiting Malassezia

[0138] This test example refers to QB / T 2738-2012 Evaluation method for antibacterial and antimicrobial effects of daily chemical products, and uses the antibacterial ring method to determine the antibacterial performance of the natural anti-dandruff composition against Malassezia.

[0139] 1. Materials and Reagents

[0140] Experimental strain: Malassezia furfur (M. furfur, ATCC44344)

[0141] Reagents: physiological saline, agar medium, peptone, glucose

[0142] Materials: culture dish, constant temperature incubator, high temperature sterilizer, etc.

[0143] 2. Operation method

[0144] 2.1 Preparation of Malassezia culture medium: Dissolve 30 g / L peptone, 20 g / L glucose, and 15 g / L agar in purified water and adjust the pH to 6.5-7.0. Autoclave at 121°C for 20 min.

[0145] 2.2 Preparation of Malassezia suspension: Take the test bacteria and subculture them for 48 hours. Add sterile sodium chloride solution to elute the culture, and pipette the bacterial suspension into a sterile test tube. Add sterile sodium chloride solution to make a bacterial suspension with a colony count of 1×105 cfu / ml, and use it on the same day.

[0146] 2.3 Prepare a sample solution (1 g / ml), dispense 20 μL onto a sterile filter paper, and allow to air dry. Following QB / T 2738-2012, apply the bacterial suspension using a sterile cotton swab to an agar plate and dry at room temperature to create a contaminated plate. Place the test specimen on the contaminated plate, incubate at 30°C for 48 hours, and observe the results. Simultaneously, perform a negative control. Repeat the test three times for each sample, evaluating the inhibition zone diameter. A diameter greater than 7 mm was considered effective. The results are shown in Table 4.

[0147] 3. Test results

[0148] The diameters of the Malassezia inhibition zones of the embodiments and comparative examples are shown in Table 5.

[0149] Table 5 Natural anti-dandruff composition inhibits Malassezia performance results

[0150]

[0151] The inhibition zone experiment demonstrates the ability of each embodiment and comparative example to inhibit Malassezia. As can be seen from the inhibition zone diameter, Example 1, which has a higher content of anti-dandruff active ingredient, has stronger antibacterial properties than Examples 2 and 3, but its antibacterial properties do not have a significant proportional relationship with the amount of active ingredient added. From the perspective of comprehensive cost, Example 3 is the best. The amount of hydrogenated lecithin added in Example 5 is relatively low, while Comparative Example 3 does not add hydrogenated lecithin and phytosterols and is a sample without liposome technology, but the amount of active ingredient added is the same as that in Example 3, so there is no significant difference in antibacterial properties. Comparative Example 1 does not add any anti-dandruff active ingredient, and its inhibition zone diameter is less than 7mm, which cannot inhibit the growth of Malassezia. Comparative Example 2 replaces the anti-dandruff active ingredient with pyridone ethanolamine salt, a commonly used chemically synthesized anti-dandruff agent on the market. Compared with each embodiment, its inhibition zone diameter does not increase much, indicating that the antibacterial properties of the natural anti-dandruff composition provided by the present invention are comparable to those of chemical anti-dandruff agents.

[0152] (IV) Evaluation of the anti-dandruff performance of natural anti-dandruff compositions in shampoo

[0153] Test shampoo samples were prepared according to the formula shown in Table 6 below:

[0154] Table 6 Content ratio of shampoo samples

[0155]

[0156]

[0157] 1. Methods

[0158] 1.1 Experimental Methods: This study employed a self-administered before-and-after control method. Subjects cleaned their scalps using a shampoo sample containing a natural anti-dandruff composition every other day for four consecutive weeks, according to their personal habits. Dandruff severity was assessed using the 10-point Adherent Scalp Flaking Scale (ASFS) on days 0, 7, and 28.

[0159] 1.2 Subjects: The test subjects were 40 volunteers without other skin diseases. The test subject conditions were as follows:

[0160] 1) Aged between 18 and 65 (excluding pregnant or breastfeeding women, or women planning to become pregnant within six months);

[0161] 2) The tested area has not been subjected to skin treatment, cosmetic treatment, or other tests that may affect the results;

[0162] 3) Those who have not used hormone drugs or immunosuppressants in the past month;

[0163] 4) The test site has not participated in other clinical trials currently or in the past three months;

[0164] 5) The subjects had varying degrees of dandruff problems, with an initial average ASFS scalp score of 6-12.

[0165] 1.3 Test environment: The test environment is maintained at a temperature of (22±2)°C and a relative humidity of (50±10)%. The subjects are required to wait quietly in this test environment for 30 minutes before the test.

[0166] 2. Evaluation Criteria

[0167] The ASFS is the most commonly used ten-point scale for adhesive dandruff in international studies. The scalp is divided into eight zones (left and right forehead, top of the head, occipital area, and neck) for scoring. Each zone is scored on a scale of 0 to 10 based on the severity of dandruff, with 0 indicating no dandruff (none), 1 to 2 indicating small, powdery, grayish-white, rough scales (minor), 3 to 4 indicating small to medium-sized scales (mild), 5 to 6 indicating large, thin scales loosely attached to the scalp (moderate), 7 to 8 indicating large, adhesive scales (significant), and 9 to 10 indicating thick, white to yellow scales tightly attached to the scalp (severe). The scores for each zone are then added together to create a total score (ranging from 0 to 80), with higher total scores indicating more severe dandruff.

[0168] Statistical methods: SPSS 19.0 statistical software was used to analyze the experimental data. P < 0.01 was considered statistically significant. ASFS scores at T0 (baseline), T1 (7 days), and T2 (28 days) were described using means and standard deviations, and statistical analysis was performed using repeated measures analysis of variance.

[0169] 3. Test results

[0170] The anti-dandruff performance test results of the shampoo containing each embodiment and comparative example are shown in Figure 1 .

[0171] Examples 1-3 all have good anti-dandruff efficacy after 7d and 28d and the anti-dandruff effect is similar. Considering the comprehensive cost, Example 3 is the best. In Example 5, due to the reduction in the amount of hydrogenated lecithin added, the liposome form of the anti-dandruff active ingredient is imperfect, and its permeability and effectiveness are worse than those of Examples 1-3, so the anti-dandruff effect is poor. Comparative Example 3 is an anti-dandruff composition in a non-liposomal form, and it also does not have a good anti-dandruff effect. This indirectly confirms that liposome technology can promote the permeability and dispersibility of active ingredients in the scalp, so that it can be more uniformly and promptly exert its efficacy when applied to the scalp, thereby improving the anti-dandruff effect. Comparative Example 2 is a commonly used anti-dandruff agent on the market. The degree of reduction in the ASFS score at 7d and 28d is not much different from the results of Examples 1-3, indicating that the natural anti-dandruff composition provided by the present invention has good anti-dandruff efficacy and can be easily applied to shampoo.

[0172] In summary, the present invention's composition is a 100% naturally derived anti-dandruff composition that inhibits Malassezia, removes dandruff, and relieves itching. By utilizing liposome technology, the effectiveness and stability of the natural plant-based anti-dandruff ingredients are enhanced, and the composition is non-irritating to the skin, making it a convenient and effective anti-dandruff composition.

[0173] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0174] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. An anti-dandruff composition, characterized in that: The composition is composed of the following components in percentage by weight: 0.1-5% of Mexican juniper oil, 0.5-8% of tartar seed oil, 1-10% of hydrogenated lecithin, 0.2-5% of phytosterol, 1-10% of natural emulsifier, 5-20% of natural plant oil, 0.2-5% of xanthan gum and the balance of water.

2. The anti-dandruff composition according to claim 1, characterized in that The composition is composed of the following components in percentage by weight: 0.1-2% of Mexican juniper oil, 0.5-5% of calendula seed oil, 2-5% of hydrogenated lecithin, 0.2-2% of phytosterol, 1-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-3% of xanthan gum and the balance of water.

3. The anti-dandruff composition according to claim 2, characterized in that The composition is composed of the following components in percentage by weight: 0.2-1% of Mexican juniper oil, 0.8-4% of tartar seed oil, 2-4.5% of hydrogenated lecithin, 0.5-2% of phytosterol, 1.5-8% of natural emulsifier, 5-20% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

4. The anti-dandruff composition according to claim 3, characterized in that The composition is composed of the following components in percentage by weight: 0.2-1% of Mexican juniper oil, 0.8-2% of tartar seed oil, 2-4.5% of hydrogenated lecithin, 0.5-1.5% of phytosterol, 1.5-6% of natural emulsifier, 5-18% of natural plant oil, 0.2-2% of xanthan gum and the balance of water.

5. The anti-dandruff composition according to any one of claims 1 to 4, characterized in that The natural emulsifier is composed of an emulsifying component and polyglyceryl-10 stearate, the weight ratio of the emulsifying component and polyglyceryl-10 stearate is (0.3-0.65):1, and the emulsifying component is composed of cetearyl alcohol and cetearyl glucoside; wherein the content of polyglyceryl-10 stearate in the anti-dandruff composition is greater than 0.6%.

6. The anti-dandruff composition according to any one of claims 1 to 4, characterized in that The natural plant oil is composed of one or more of sunflower seed oil, cobaiba oil, bacas seed oil and sweet almond oil.

7. The anti-dandruff composition according to any one of claims 1 to 4, characterized in that The phytosterols are composed of one or both of β-sitosterol and stigmasterol.

8. A method for preparing the anti-dandruff composition according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Mixing juniper oil and calendula seed oil to obtain a first intermediate; dissolving hydrogenated lecithin and phytosterol in a solvent to obtain a second intermediate; S2: adding the first intermediate to the second intermediate, and removing the solvent to obtain a third intermediate; S3: adding a buffer solution to the third intermediate, dispersing the mixture, and filtering the mixture with a microporous membrane to obtain liposomes containing juniper oil and calamagrostis seed oil; S4: dissolving xanthan gum in an aqueous solvent and maintaining the solution at a first temperature; adding vegetable oil and natural emulsifier to the oil solvent, heating the solution to the first temperature until dissolved, and then adding the solution to the aqueous solvent and homogenizing the solution to obtain an emulsion; S5: After the emulsion is cooled to below the second temperature, the juniper oil and calendula seed oil liposomes obtained in step S3 are added to obtain the anti-dandruff composition.

9. A nursing product, characterized in that: Contains the anti-dandruff composition according to any one of claims 1 to 7.

10. The care product according to claim 9, characterized in that The care products are shampoo products.

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