An anti-aging plant composition and its preparation method and application

Abstract

The application discloses an anti-aging plant composition and a preparation method and application thereof, and belongs to the technical field of daily-use cosmetic compounding materials. The composition contains, in mass parts, 5-10 parts of peony root extract, 1-6 parts of golden yellow chamomile extract, 5-7 parts of pelargonium graveolens flower / leaf / stem extract, 0.5-1.5 parts of drynaria rhizome extract and 1-3 parts of gypsophila oldhamiana extract. The drynaria rhizome is extracted by an ultrasonic-reflux combined process, and the gypsophila oldhamiana is extracted by a composite enzymolysis-ethanol reflux method. The composition can realize multi-pathway anti-aging by removing free radicals, inhibiting the generation of AGEs, down-regulating MMP-1 and promoting collagen synthesis. The composition can be added to a serum, and by cooperating with moisturizing agents, conditioning agents and the like, the effective use of the efficacy can be realized.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of daily-use cosmetic compound raw materials, and particularly relates to an anti-aging plant composition and a preparation method and application thereof. BACKGROUND

[0002] Skin aging is the result of the joint action of internal genes and external environment (such as ultraviolet rays and glycation), and is mainly manifested in wrinkle proliferation, loss of elasticity and dark skin.

[0003] Current anti-aging ingredients have limitations:

[0004] 1. Chemical synthetic ingredients: such as retinol, which is highly irritating and easy to cause peeling and erythema;

[0005] 2. Single plant extract: the efficacy dimension is single, such as peony root extract which focuses on antioxidant, and gold yellow chamomile extract which focuses on anti-inflammatory, and it is difficult to comprehensively resist multi-pathway aging mechanisms such as photoaging and glycation;

[0006] 3. Traditional extraction process: high temperature or strong solvent can easily destroy heat-sensitive active ingredients and reduce bioavailability.

[0007] Therefore, it is urgent to develop a plant compound composition with multiple targets, high activity and low irritation to improve the comprehensive anti-aging efficiency through synergistic effect. SUMMARY

[0008] In view of the deficiencies of the prior art, the purpose of the present application is to provide an anti-aging plant composition with multiple dimensions and multiple pathways for effective antioxidant, anti-glycation, inhibition of collagen decomposition and promotion of collagen synthesis, and a preparation method thereof.

[0009] In order to achieve the above-mentioned purpose, the following technical solutions are disclosed:

[0010] In the first aspect, the present application provides an anti-aging plant composition, which comprises the following components in mass fraction:

[0011] Peony root extract 5-10 parts;

[0012] Gold yellow chamomile extract 1-6 parts;

[0013] Rosa rubiginosa flower / leaf / stem extract 5-7 parts;

[0014] Drynaria root extract 0.5-1.5 parts;

[0015] Gold-edged lotus extract 1-3 parts.

[0016] Peony root extract can effectively scavenge free radicals and fight photoaging, and has excellent antioxidant capacity. It significantly inhibits multiple inflammatory factors, relieves redness and stinging, and has a powerful anti-inflammatory and soothing effect. It can also inhibit tyrosinase activity, interfere with melanin production and transfer, thereby whitening and removing spots, brightening skin tone, and evening skin tone. It also has the effects of inhibiting collagen degrading enzymes, promoting collagen production, improving wrinkle relaxation, and resisting glycation.

[0017] Golden chamomile extract is rich in active ingredients such as matricaria, which can quickly calm the skin and relieve sensitive discomfort such as redness, stinging, and itching. It inhibits the release of histamine, reduces the production of inflammatory factors, effectively repairs damaged skin barriers, and improves skin tolerance. It also has excellent antioxidant capacity, fights free radical damage, and gently nourishes the skin, making it especially suitable for recovery after stimulation.

[0018] Pelargonium graveolens flower / leaf / stem extract has the effect of significantly regulating oil secretion and tightening pores, and has natural antibacterial and anti-inflammatory properties. At the same time, it is rich in antioxidants to help resist free radical damage.

[0019] Preferably, the preparation method of the Pteris chinensis rhizome extract comprises the following steps:

[0020] 1-1. The rhizome of the oak fern is dried and crushed and sieved to obtain oak fern powder;

[0021] 1-2. Soak the powdered fern in a 90 v / v% ethanol-water solution at a solid-liquid ratio of 1:(5-8) g / mL. Ultrasonicate at 25-30°C and 20-30 kHz for 50-60 min, then centrifuge to obtain a supernatant for later use. Mix the remaining precipitate with a 70 v / v% ethanol-water solution at a solid-liquid ratio of 1:(10-12) g / mL and extract under reflux at 60-70°C for 2-3 times, each time for 0.5-0.7 h. Combine the resulting extract with the supernatant to obtain a total extract.

[0022] 1-3. The total extract was concentrated by vacuum distillation until there was no alcohol taste to obtain a concentrate, which was then dried by spray drying to obtain a Pteris chinensis rhizome extract.

[0023] Further preferably, the sieve mesh number in step 1-1 is ≥60 mesh.

[0024] More preferably, the conditions for the reduced pressure distillation in step 1-3 are a temperature of 40 to 50° C. and a pressure of 0.01 to 0.05 MPa.

[0025] Further preferably, the spray drying conditions in steps 1-3 are an inlet air temperature of 165-175°C and an outlet air temperature of 50-70°C.

[0026] The rhizome extract of Quercus oleifera is rich in flavonoids and their glycosides, alkaloids, sesquiterpenes, steroids and their saponins, and phenolic compounds, and has excellent antioxidant, anti-glycation, anti-inflammatory, and antibacterial effects. The rhizome extract of Quercus oleifera obtained by the above-mentioned extraction method has comprehensive active ingredients, is extracted using non-toxic solvents, and is highly safe. The present invention has found that under the formula system provided by the present invention, the rhizome extract of Quercus oleifera exhibits a certain effect of promoting collagen synthesis.

[0027] Preferably, the preparation method of the Herba Lycopodii extract comprises the following steps:

[0028] 2-1. Take the golden lotus and dry it to constant weight, grind it through a 60 mesh sieve to obtain golden lotus powder;

[0029] 2-2. Take the golden lotus powder, add deionized water at a material-liquid ratio of 1: (10-20) g / mL, stir and disperse, then add 10-15% of the total weight of the material-liquid complex enzyme, shake in a constant temperature water bath at 35-40℃ for 4-5 hours, and after the enzymatic hydrolysis is completed, heat to 100℃ in a water bath for 10-15 minutes to terminate the enzymatic hydrolysis, and cool to room temperature to obtain the enzymatic hydrolyzate;

[0030] 2-3. Measure a certain amount of enzymatic hydrolysate and 95 v / v% ethanol aqueous solution, mix them evenly in a volume ratio of 1:(3-4), extract under reflux in a water bath at 70-80°C three times for 1 hour each time, and combine the extracts;

[0031] 2-4. The obtained extract is concentrated by vacuum distillation until there is no alcohol taste to obtain a concentrate, and the concentrate is spray-dried into a powder to obtain a golden lily extract.

[0032] Further preferably, the complex enzyme has a mannanase activity of 500-600 U / g, a pectinase activity of 1000-1500 U / g, and an acid protease activity of 200-300 U / g.

[0033] More preferably, the conditions for the reduced pressure distillation in step 2-4 are a temperature of 40 to 50° C. and a pressure of 0.01 to 0.05 MPa.

[0034] Further preferably, the spray drying conditions in steps 2-4 are an inlet air temperature of 165-175°C and an outlet air temperature of 50-70°C.

[0035] The golden peony extract obtained by the golden peony extraction method provided by the present invention has a strong antioxidant effect, can effectively scavenge free radicals, and protect the skin from free radical damage. At the same time, the research of this application found that in the above-mentioned formula system, the golden peony extract combined with other ingredients can effectively promote collagen synthesis, thereby improving the anti-aging performance of the formula system with peony root extract as the core ingredient.

[0036] In a second aspect, the present invention provides use of the composition described in the first aspect in preparing a skin care product with firming and anti-wrinkle effects.

[0037] In a third aspect, the present invention provides a firming and anti-wrinkle essence, wherein the essence comprises the composition described in the first aspect.

[0038] Preferably, the composition is added to the essence in an amount of 0.2-1 wt %.

[0039] More preferably, the essence further comprises a moisturizer, a preservative, an emulsifier, a thickener, a conditioner, an antioxidant, a solvent, and a fragrance.

[0040] More preferably, the moisturizing agent is at least one of glycerin, butylene glycol, 1,2-hexanediol, allantoin, sodium hyaluronate, 1,2-pentanediol, propylene glycol, and glyceryl caprylate;

[0041] The preservative is at least one of p-hydroxyacetophenone and octanoylhydroxamic acid;

[0042] The emulsifier is at least one of PEG / PPG-17 / 6 copolymer, PPG-26-butanediol polyether-26, and PEG-40 hydrogenated castor oil;

[0043] The thickener is at least one of xanthan gum and polyvinyl pyrrolidone;

[0044] The conditioning agent is at least one of ergothioneine, aloe vera extract, niacinamide, decarboxylated carnosine hydrochloride, bifid yeast fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, hydrolyzed yeast extract, sophora flavescens root extract, purslane extract, and field mint leaf extract;

[0045] The antioxidant is at least one of astaxanthin and fullerene.

[0046] Beneficial effects of the present invention:

[0047] 1. The present invention achieves excellent antioxidant, anti-glycation, collagen degradation inhibition and collagen synthesis promotion effects by compounding peony root extract, golden chamomile extract, geranium flower / leaf / stem extract, Quercus rhizome extract and golden lotus extract, thereby delaying skin aging in multiple dimensions.

[0048] 2. The present invention adopts a composite enzymatic hydrolysis / ethanol / water extraction process to avoid toxic solvents, ensure the integrity of the active ingredients, and meet the requirements of mildness, low irritation, and high safety. DETAILED DESCRIPTION

[0049] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0050] To further illustrate the present invention, the following examples are provided for detailed description. The raw materials used in the following examples and comparative examples are all commercially available products; the raw material companies listed below are one of the sources for purchasing the raw materials.

[0051] Peony root extract: purchased from Shanghai Ruidian Biotechnology Co., Ltd.

[0052] Golden chamomile extract: purchased from NCKemical Biotechnology SL;

[0053] Pelargonium graveolens flower / leaf / stem extract: purchased from NCKemical Biotechnology SL;

[0054] Drynaria rhizome: the rhizome of the Drynaria species (Drynaria roosii Nakaike) of the genus Drynaria;

[0055] Golden Lotus: The whole plant of the Goodyera schlechtendaliana Rchb. F.

[0056] Preparation of extract from Quercus truncatula rhizome

[0057] 1-1. The rhizomes of the oak fern were dried and placed in a high-speed universal grinder and crushed through a 60-mesh sieve to obtain oak fern powder;

[0058] 1-2. Soak the fern powder in a 90 v / v% ethanol aqueous solution at a solid-liquid ratio of 1:8 g / mL, ultrasonically treat at 30 ° C, 28 kHz for 50 min, and then centrifuge to obtain the supernatant for later use. The remaining precipitate was mixed with a 70 v / v% ethanol aqueous solution at a solid-liquid ratio of 1:12 g / mL and refluxed at 70 ° C for 3 times, each time for 0.7 h. The obtained extract was combined with the supernatant to obtain the total extract;

[0059] 1-3. The total extract was concentrated by vacuum distillation at 50°C and a pressure of 0.01 MPa until there was no alcohol taste to obtain a concentrate, which was then dried by spray drying under the conditions of an inlet air temperature of 175°C and an outlet air temperature of 70°C to obtain a fern rhizome extract.

[0060] Preparation of Golden Lotus Extract

[0061] 2-1. Take the golden lotus and dry it to constant weight, grind it through a 60-mesh sieve to obtain golden lotus powder;

[0062] 2-2. Take golden lotus powder, add deionized water at a material-liquid ratio of 1:20g / mL, stir and disperse, then add 10-15% of the total mass of the feed solution of the complex enzyme, shake in a constant temperature water bath at 40 ° C for 5h, after the enzymatic hydrolysis is completed, heat to 100 ° C in a water bath for 15min to terminate the enzymatic hydrolysis, and cool to room temperature to obtain an enzymatic solution, wherein the complex enzyme contains mannanase activity 500U / g, pectinase activity 1000U / g, acid protease activity 200U / g;

[0063] 2-3. Measure a certain amount of enzymatic hydrolysate and 95v / v% ethanol aqueous solution, mix them in a volume ratio of 1:4, extract them in a water bath under reflux at 75°C three times for 1 hour each time, and combine the extracts;

[0064] 2-4. The obtained extract was concentrated by vacuum distillation at 50°C and a pressure of 0.01 MPa until there was no alcohol taste to obtain a concentrate, which was spray-dried into a powder to obtain a golden lily extract. The spray-drying conditions were an inlet air temperature of 168°C and an outlet air temperature of 67°C.

[0065] Preparation of anti-aging plant composition mother solution

[0066] The raw materials were accurately weighed according to their mass fractions and mass percentages in Table 1, mixed, and homogenized and dispersed uniformly at 10,000 r / min to obtain a mother solution of the anti-aging plant composition.

[0067] Table 1

[0068]

[0069] The raw materials were accurately weighed according to their mass fractions and mass percentages in Table 2, and after mixing, they were homogenized and dispersed evenly at 10,000 r / min to obtain a comparative mother liquor. The comparative mother liquor was based on the formula of the composition mother liquor 3, and the missing parts were supplemented with peony root extract to verify the effect of the missing ingredients on the performance of the overall formula.

[0070] Table 2

[0071]

[0072] Note: “ / ” in the table means no additive.

[0073] Performance Testing

[0074] The mother solutions 1-4 and the comparative mother solutions 1-4 prepared above were subjected to the following anti-aging performance tests on free radical scavenging, anti-glycation, MMP-1 inhibition, and promotion of collagen synthesis.

[0075] 1 DPPH free radical scavenging

[0076] The DPPH ethanol solution is violet and has a strong absorbance at 517 nm. If it combines with the sample solution to be tested, the absorbance value at 517 nm will be reduced, thereby judging the ability of the sample solution to be tested to scavenge DPPH free radicals.

[0077] (1) Sample liquid to be tested

[0078] The composition mother liquor 1-4 and the comparison mother liquor 1-4 were diluted with deionized water at a mass ratio of 1:9 to obtain test sample solutions 1-8 with a solute concentration of 10 wt %, respectively.

[0079] (2) Experimental design

[0080] DPPH was prepared with anhydrous ethanol to prepare 2×10 -4 mol / L DPPH solution, take several 2mL portions of the sample solution to be tested, DPPH solution, and anhydrous ethanol, mix 2mL of the sample solution to be tested and 2mL of DPPH solution, then place them at room temperature for 30min, and measure the absorbance at a wavelength of 517nm to obtain Ai;

[0081] Mix 2 mL of the sample solution to be tested and 2 mL of anhydrous ethanol and measure the absorbance as Aj according to the above method;

[0082] Mix 2 mL of DPPH solution and 2 mL of anhydrous ethanol and measure the absorbance as Ac according to the above method.

[0083] Each sample was measured three times in parallel and the average value was taken.

[0084] The scavenging rate of DPPH free radicals of each test sample was calculated according to formula (1) and recorded in Table 3 below.

[0085] Clearance rate (%) = (1-(Ai-Aj) / Ac) × 100%...... (1)

[0086] Where: Ai is the absorbance of a mixture of 2 mL of the sample solution to be tested and 2 mL of DPPH solution; Aj is the absorbance of a mixture of 2 mL of the sample solution to be tested and 2 mL of anhydrous ethanol; Ac is the absorbance of a mixture of 2 mL of DPPH solution and 2 mL of anhydrous ethanol.

[0087] Table 3 DPPH clearance rate

[0088] Group DPPH clearance rate / % Test sample solution 1 (containing 10% composition stock solution 1) 89.64 Test sample solution 2 (containing 10% composition stock solution 2) 88.11 Test sample solution 3 (containing 10% composition stock solution 3) 90.57 Test sample solution 4 (containing 10% composition stock solution 4) 88.39 Test sample solution 5 (containing 10% comparison mother solution 1) 67.53 Test sample solution 6 (containing 10% comparison mother solution 2) 54.93 Test sample solution 7 (containing 10% comparison mother solution 3) 72.67 Test sample solution 8 (containing 10% comparison mother solution 4) 58.02

[0089] 2 Anti-glycation test

[0090] Under non-enzymatic conditions, aldehyde-containing sugars react with protein amino residues to form Schiff bases, which then evolve into early glycation products and highly reactive carbonyl compounds, ultimately forming advanced glycation end products (AGEs). The accumulation of AGEs damages the skin, causing non-enzymatic glycation and cross-linking of dermal collagen and elastin, leading to decreased skin elasticity, wrinkling, reduced tissue permeability, and a gradual darkening of the skin tone. Therefore, anti-glycation testing, assessing the ability of a test sample to inhibit AGE formation, can be used to evaluate its anti-aging efficacy.

[0091] (1) Experimental plan

[0092] Test sample solution: The composition mother solution 1-4 and the reference mother solution 1-4 were diluted with deionized water at a mass ratio of 1:9 to obtain test sample solutions 1-8 with a solute concentration of 10 wt %.

[0093] Assay method: A mixed solution containing 80 mg / mL bovine serum albumin and 240 mg / mL glucose was prepared in 0.1 mol / L PBS and filtered through a 0.22 μm filter to serve as the saccharification reaction solution. Reaction systems A, B, C, and D were prepared according to Table 4. The final concentration of bovine serum albumin in each reaction system was 40 mg / mL, and the final concentration of glucose was 120 mg / mL.

[0094] Table 4 Preparation of reaction systems for each group

[0095] raw material A B C D Saccharification reaction solution / mL 1 0 1 0 Sample solution or positive control solution / mL 1 1 0 0 <![CDATA[0.1mol·L -1 PBS solution / mL]]> 0 1 1 2

[0096] After mixing thoroughly, the mixture was incubated at 55°C for 4 days, using 1% aminoguanidine hydrochloride as a positive control. After the reaction, the incubation solution was cooled to room temperature and assayed. 200 μL of the reaction solution was then added to a 96-well plate. The AGE inhibition rate was measured using a fluorescence microplate reader at an excitation wavelength of 370 nm and an emission wavelength of 440 nm. The results are shown in Table 5.

[0097] (2) Calculation of AGEs inhibition rate

[0098] Calculation formula:

[0099] AGEs inhibition rate (%) = [1-(AB) / (CD)] × 100%.......(2)

[0100] Where: fluorescence intensity of system A; fluorescence intensity of system B; fluorescence intensity of system C; fluorescence intensity of system D.

[0101] (3) Test results

[0102] Table 5 AGEs detection results

[0103] Group AGEs inhibition rate (%) Positive control solution 82.93 Test sample solution 1 (containing 10% composition stock solution 1) 41.23 Test sample solution 2 (containing 10% composition stock solution 2) 43.87 Test sample solution 3 (containing 10% composition stock solution 3) 44.92 Test sample solution 4 (containing 10% composition stock solution 4) 40.56 Test sample solution 5 (containing 10% comparison mother solution 1) 30.16 Test sample solution 6 (containing 10% comparison mother solution 2) 22.84 Test sample solution 7 (containing 10% comparison mother solution 3) 35.00 Test sample solution 8 (containing 10% comparison mother solution 4) 27.43

[0104] 3 MMP-1 inhibition assay

[0105] MMP-1 can shorten the fiber structure of collagen, leading to collagen degradation and tissue remodeling, resulting in signs of aging such as skin sagging and wrinkles. By reducing the activity of MMP-1, the formation of wrinkles and skin aging can be delayed.

[0106] (1) Cell preparation and inoculation

[0107] Normal human skin fibroblasts (HSF) in the logarithmic growth phase were obtained and the adherent cells were digested with 0.25% trypsin solution to make them fall off. After counting, the cell suspension density was adjusted to 4×10 5 The cell suspension was seeded into a 6-well plate.

[0108] (2) Group treatment and UVB irradiation

[0109] Normal control group: Add 200 μL of PBS and supplement with DMEM medium to a total volume of 800 μL. No UV irradiation was performed.

[0110] Model group and experimental group: Wash the cells gently with an appropriate amount of PBS until the washing solution is colorless. After discarding the PBS, add 200 μL PBS to cover the cells. Place the 6-well plates of the model group and experimental group under UVB light for 15 minutes (cumulative irradiation dose: 40 mJ / cm 2 After irradiation, discard the PBS.

[0111] Model group: PBS solution was added and DMEM medium was supplemented to a total volume of 800 μL.

[0112] Experimental group: DMEM culture medium containing 1 wt % of composition mother solutions 1-4 and control mother solutions 1-4 was added to a total volume of 800 μL.

[0113] Incubation: All groups (normal control group, model group, experimental group) were placed in a 37°C, 5v / v% CO2 incubator for 48h.

[0114] (3) Sample collection and MMP-1 detection:

[0115] After the incubation period, the culture supernatant was collected and centrifuged. The supernatant was discarded, and RIPA lysis buffer was added to the cell pellet. The cells were thoroughly mixed using a vortexer for 30 seconds each time, three times, with 3 minutes between each rotation (keep on ice). The cells were centrifuged at 12,000 rpm for 10 minutes at 4°C. The supernatant was carefully aspirated, and the procedure was performed strictly according to the MMP-1 ELISA kit instructions. Within 15 minutes after adding the stop solution, the absorbance (OD) of each well was measured at 450 nm using a microplate reader, and the MMP-1 content was calculated. The results are shown in Table 6.

[0116] Table 6 MMP-1 content in each group

[0117]

[0118] 4. Test to promote type I collagen synthesis

[0119] This experiment investigated the promoting effect of composition mother liquor 1-4 and control mother liquor 1-4 on collagen secretion, with specific reference to T / SHRH 031 2020 "Determination of type I collagen content in fibroblasts in vitro for cosmetic firming and anti-wrinkle efficacy test". The positive control group was a cell culture medium containing 100 ng / mL TGFβ1, and the experimental group was a cell culture medium containing 1 wt% composition mother liquor 1-4 and control mother liquor 1-4. The growth rate of type I collagen was calculated according to the formula: growth rate (%) = (experimental group or positive group - blank group) / blank group. The experimental results are shown in Table 7.

[0120] Table 7 Type I collagen content detection results

[0121]

[0122] Result analysis:

[0123] (1) Free radical scavenging ability (DPPH)

[0124] The clearance rates of the complete formula composition mother solutions 1-4 were all greater than 88%, which were significantly higher than those of the control mother solutions 1-4, indicating that the five components of peony root extract, golden chamomile extract, geranium flower / leaf / stem extract, quercus rhizome extract and golden lotus extract work synergistically to greatly enhance the antioxidant efficacy. Among them, the clearance rate was lowest when the geranium flower / leaf / stem extract was missing, confirming its key contribution to antioxidant effects.

[0125] (2) Anti-glycation effect

[0126] The inhibition rates of the mother solutions 1-4 of the complete formula group were 40.56%-44.92%, among which the AGEs inhibition rate of the mother solution 3 reached 44.92%, which was significantly better than that of the missing group. This proves that golden chamomile extract, pelargonium flower / leaf / stem extract, oak fern rhizome extract and golden lotus extract have the effect of promoting the anti-glycation effect of peony root extract in the formula system with peony root extract as the core ingredient, and the lack of any one of them will lead to a decline in the performance of the overall formula.

[0127] (3) Anti-wrinkle mechanism

[0128] After UVB damage, MMP-1 levels in the complete formula group (99.47-103.71 pg / mL) approached those in the normal control group (43.51 pg / mL) and were 40.2%-42.6% lower than those in the model group (173.44 pg / mL). MMP-1 levels in the missing formula groups (127.19-149.62 pg / mL) were significantly higher, with the group lacking the golden lily (compared to mother solution 4) showing the lowest level (149.62 pg / mL), indicating that golden lily plays a crucial role in inhibiting collagen degradation.

[0129] (4) Promote collagen synthesis

[0130] The complete formula group showed collagen growth rates of 45.27%-53.15%, while the missing group showed only 13.49%-21.89%. The groups missing Quercus ferns and Herba Lycoris lobata showed the lowest growth rates compared to mother solutions 3 and 4, at 19.79% and 13.49% respectively, confirming the irreplaceable role of the two ingredients in the formula in synergistically promoting collagen synthesis.

[0131] Preparation of firming and anti-wrinkle essence

[0132] Weigh the raw materials according to the mass percentages in Table 8;

[0133] Step 1. Heat deionized water to 70-75°C, add a humectant and a thickener in sequence, stir until completely dissolved, and keep warm for 20 minutes to obtain a mixture A;

[0134] Step 2. Mix the ingredients in the emulsifier and heat to 70-75°C to dissolve evenly to obtain mixture B;

[0135] Step 3. Slowly add mixture B to mixture A and homogenize at 5000-8000 rpm for 5 minutes. After cooling to 40-45°C, add the conditioning agent, preservative, antioxidant, and anti-aging plant composition mother solution 3 and homogenize at 5000-8000 rpm for 3 minutes.

[0136] Step 4. When the temperature drops to 30-35°C, add daily fragrance, cool to room temperature, filter through a 200-mesh filter, and aseptically fill into a light-proof container to obtain a firming and anti-wrinkle essence.

[0137] Table 8 Raw materials of firming and anti-wrinkle essence

[0138]

[0139] The foregoing description of the disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An anti-aging plant composition, characterized in that: The composition consists of the following components in parts by mass: 5-10 parts of peony root extract; 1-6 parts of golden chamomile extract; 5-7 parts of Pelargonium graveolens flower / leaf / stem extract; 0.5-1.5 parts of Quercus truncatula rhizome extract; 1-3 parts of golden lotus extract; The preparation method of the fern rhizome extract comprises the following steps: 1-1. The rhizome of the oak fern is dried and crushed and sieved to obtain oak fern powder; 1-2. Soak the powdered fern in a 90 v / v% ethanol-water solution at a solid-liquid ratio of 1:(5-8) g / mL. Ultrasonicate at 25-30°C and 20-30 kHz for 50-60 min, then centrifuge to obtain a supernatant for later use. Mix the remaining precipitate with a 70 v / v% ethanol-water solution at a solid-liquid ratio of 1:(10-12) g / mL and extract under reflux at 60-70°C for 2-3 times, each time for 0.5-0.7 h. Combine the resulting extract with the supernatant to obtain a total extract. 1-3. The total extract was concentrated by distillation under reduced pressure until there was no alcohol taste to obtain a concentrate, which was spray-dried to obtain a Quercus rhizome extract; The preparation method of the golden lily extract comprises the following steps: 2-1. Take the golden lotus and dry it to constant weight, grind it through a 60 mesh sieve to obtain golden lotus powder; 2-2. Take the golden lotus powder, add deionized water at a material-liquid ratio of 1: (10-20) g / mL, stir and disperse, then add 10-15% of the total weight of the material-liquid complex enzyme, shake in a constant temperature water bath at 35-40℃ for 4-5 hours, and after the enzymatic hydrolysis is completed, heat to 100℃ in a water bath for 10-15 minutes to terminate the enzymatic hydrolysis, and cool to room temperature to obtain the enzymatic hydrolyzate; 2-3. Measure a certain amount of enzymatic hydrolysate and 95 v / v% ethanol aqueous solution, mix them evenly in a volume ratio of 1:(3-4), extract under reflux in a water bath at 70-80°C three times for 1 hour each time, and combine the extracts; 2-4. The obtained extract was concentrated by vacuum distillation until there was no alcohol taste to obtain a concentrate, which was spray-dried into a powder to obtain a golden lotus extract; The complex enzyme has an enzymatic activity of mannanase of 500-600 U / g, an enzymatic activity of pectinase of 1000-1500 U / g, and an enzymatic activity of acid protease of 200-300 U / g.

2. The composition according to claim 1, characterized in that The sieve mesh number in step 1-1 is ≥60 mesh; The conditions for the reduced pressure distillation in steps 1-3 are a temperature of 40-50° C. and a pressure of 0.01-0.05 MPa; The spray drying conditions in steps 1-3 are an inlet air temperature of 165-175°C and an outlet air temperature of 50-70°C.

3. The composition according to claim 1, characterized in that The conditions for the reduced pressure distillation in step 2-4 are a temperature of 40-50° C. and a pressure of 0.01-0.05 MPa; The spray drying conditions are as follows: an inlet air temperature of 165-175°C and an outlet air temperature of 50-70°C.

4. Use of the composition according to any one of claims 1 to 3 in preparing a skin care product with firming and anti-wrinkle effects.

5. A firming and anti-wrinkle essence, characterized in that: The essence contains the composition according to any one of claims 1 to 3; The composition is added to the essence in an amount of 0.2-1 wt %.

6. The essence according to claim 5, characterized in that The essence also contains moisturizer, preservative, emulsifier, thickener, conditioner, antioxidant, solvent and essence.

7. The essence according to claim 6, characterized in that The moisturizing agent is at least one of glycerin, butylene glycol, 1,2-hexanediol, allantoin, sodium hyaluronate, 1,2-pentanediol, propylene glycol, and glyceryl caprylate; The preservative is at least one of p-hydroxyacetophenone and octanoylhydroxamic acid; The emulsifier is at least one of PEG / PPG-17 / 6 copolymer, PPG-26-butanediol polyether-26, and PEG-40 hydrogenated castor oil; The thickener is at least one of xanthan gum and polyvinyl pyrrolidone; The conditioning agent is at least one of ergothioneine, aloe vera extract, niacinamide, decarboxylated carnosine hydrochloride, bifid yeast fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, hydrolyzed yeast extract, sophora flavescens root extract, purslane extract, and field mint leaf extract; The antioxidant is at least one of astaxanthin and fullerene.

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