Traditional Chinese medicine composition for reinforcing qi and nourishing blood as well as preparation method and detection method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for reinforcing qi and nourishing blood and a detection method thereof. The traditional Chinese medicine composition is prepared from ginseng, astragalus membranaceus, Chinese yam, lily, colla corii asini, roasted rhizoma atractylodis macrocephalae, pulp of dogwood fruit, ligustrum lucidum, schisandra chinensis, pericarpium citri reticulatae, rhizoma anemarrhenae, cornu cervi pantotrichum and lycium barbarum. According to the theory of traditional Chinese medicine, the prescription is screened by adopting the theory of treatment based on syndrome differentiation, and the medicines are scientifically and reasonably matched, have the effects of reinforcing qi, nourishing blood, consolidating the body resistance and strengthening the body resistance, and are used for treating shortness of breath, palpitation, lusterless complexion, weakness and weakness caused by deficiency of qi and blood. In addition, the traditional Chinese medicine composition can improve the body immunity of patients with deficiency of qi and blood, and can also be used for reducing adverse reactions such as myelosuppression and thrombocytopenia caused by radiotherapy and chemotherapy. Content determination is carried out through TLC identification and HPLC, and a scientific basis is provided for guaranteeing the clinical quality and effectiveness of reinforcing qi, nourishing blood and strengthening body resistance.

Description

Technical Field

[0001] The present invention relates to a Chinese medicine composition, in particular to a Chinese medicine composition for invigorating qi and nourishing blood, and a preparation method and a detection method thereof. Background Art

[0002] Qi and blood deficiency is a common constitutional problem in Traditional Chinese Medicine (TCM), often caused by insufficient congenital endowment or a variety of acquired factors. Congenitally weak constitutions and insufficient congenital endowment, such as weak parents, malnutrition during pregnancy, or premature birth, can lead to a weak foundation for the production of Qi and blood. Acquired factors, including nutritional imbalance: long-term dieting, partial eating (such as excessive vegetarianism), insufficient protein or iron intake, affect the production of Qi and blood. Damage to the spleen and stomach: overeating, a preference for raw and cold foods (such as iced drinks and cold foods), or excessive weight loss can lead to spleen and stomach deficiency and coldness, affecting the "source of Qi and blood production." Lack of sleep: Prolonged late nights and insomnia prevent Qi and blood from being repaired at night. Emotional disorders: Long-term stress, anxiety, and depression lead to liver Qi stagnation, Qi stagnation and blood stasis, hindering the circulation of Qi and blood; excessive sadness depletes Lung Qi. Chronic diseases: such as digestive system diseases (gastritis, enteritis) that affect nutrient absorption; chronic bleeding (hemorrhoids, heavy menstruation); failure to recuperate in time after major illness; patients with cancer diseases who become physically weak after radiotherapy and chemotherapy, and also experience adverse reactions such as bone marrow suppression. Summary of the Invention

[0003] Purpose of the invention: The purpose of the present invention is to address the deficiencies in the existing technology, screen the composition and dosage of Chinese medicine based on the theory of traditional Chinese medicine and the theory of syndrome differentiation and treatment, and use thin layer chromatography and high performance liquid chromatography to qualitatively identify and determine the active ingredients.

[0004] The experimental results show that the Chinese medicine composition provided by the present invention has the effects of invigorating qi and nourishing blood, strengthening the body and strengthening the foundation, and has a good therapeutic effect on shortness of breath, palpitations, sallow complexion, physical weakness and fatigue caused by qi and blood deficiency.

[0005] In addition, the Chinese medicine composition of the present invention can also enhance the body's immunity, reduce adverse reactions such as bone marrow suppression, and has multiple other effects.

[0006] Technical solution: In order to achieve the above objectives, the technical solution adopted by the present invention is: A traditional Chinese medicine composition for invigorating qi and nourishing blood, comprising the following traditional Chinese medicine raw materials: Ginseng, astragalus, yam, lily, donkey-hide gelatin, white atractylodes, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, deer antler, and wolfberry.

[0007] As a preferred embodiment, the above-mentioned Chinese medicine composition for invigorating qi and nourishing blood is prepared from the following Chinese medicine raw materials in parts by weight: 8-24 parts of ginseng, 80-240 parts of astragalus, 75-225 parts of yam, 50-150 parts of lily, 30-90 parts of donkey-hide gelatin, 30-90 parts of stir-fried atractylodes, 30-90 parts of cornus fruit, 30-90 parts of privet, 25-75 parts of schisandra, 30-90 parts of dried tangerine peel, 25-75 parts of anemarrhena, 1.5-4.5 parts of deer antler, and 50-150 parts of wolfberry.

[0008] As a preferred embodiment, the above-mentioned Chinese medicine composition for invigorating qi and nourishing blood is prepared from the following Chinese medicine raw materials in parts by weight: 8-16 parts of ginseng, 80-160 parts of astragalus, 75-150 parts of yam, 50-100 parts of lily, 30-60 parts of donkey-hide gelatin, 30-60 parts of stir-fried atractylodes, 30-60 parts of cornus fruit, 30-60 parts of privet, 25-50 parts of schisandra, 30-60 parts of dried tangerine peel, 25-50 parts of anemarrhena, 1.5-3.0 parts of deer antler, and 50-100 parts of wolfberry.

[0009] As a preferred embodiment, the above-mentioned Chinese medicine composition for invigorating qi and nourishing blood is prepared from the following Chinese medicine raw materials in parts by weight: 10 parts of ginseng, 85 parts of astragalus, 75 parts of yam, 50 parts of lily, 35 parts of donkey-hide gelatin, 35 parts of stir-fried atractylodes, 35 parts of cornus fruit, 30 parts of privet, 25 parts of schisandra, 35 parts of dried tangerine peel, 25 parts of anemarrhena, 2 parts of deer antler, and 50 parts of wolfberry.

[0010] The method for preparing the traditional Chinese medicine composition for invigorating qi and nourishing blood of the present invention comprises the following steps: (1) Deer antlers are boiled in water according to weight to obtain an extract, which is then combined, concentrated, and ethanol is added. The mixture is allowed to stand for precipitation, filtered, and the filtrate is concentrated to obtain a deer antler extract for later use; (2) Take ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry according to weight, add water and boil to extract, combine the extracts, concentrate, and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing a traditional Chinese medicine preparation.

[0011] As a preferred embodiment, the preparation method of the above-mentioned traditional Chinese medicine composition for invigorating qi and nourishing blood comprises the following steps: (1) Take velvet antler by weight, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, to obtain the extract, combine and concentrate to obtain a concentrate with a relative density of 1.2 to 1.25 at 20°C, add 3 times the amount of ethanol to the concentrate, let it stand for 24 to 48 hours, precipitate, filter, take the filtrate, concentrate, and obtain the velvet antler extract for later use; (2) Take ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, white atractylodes, cornus fruit, privet, schisandra chinensis, dried tangerine peel, anemarrhena asphodeloides and wolfberry according to their weight, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, combine the decoction extracts, concentrate and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing a traditional Chinese medicine preparation.

[0012] As a preferred embodiment, the method for preparing the above-mentioned traditional Chinese medicine composition for invigorating qi and nourishing blood, wherein the traditional Chinese medicine preparation includes dosage forms such as oral liquid, tablets, capsules, granules, pills, mixtures, powders or extracts.

[0013] A method for detecting a traditional Chinese medicine composition for invigorating qi and nourishing blood, characterized by comprising qualitative identification by thin layer chromatography and content determination; The said thin layer chromatography qualitative identification includes the identification of astragaloside IV and ginseng as a reference medicinal material; The identification of astragaloside IV includes the following steps: (1) Preparation of test solution Take antler velvet by weight, add water to decoct and extract to obtain an extract, combine, concentrate, add ethanol, let stand, precipitate, filter, take the filtrate, concentrate, and obtain antler velvet extract; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry by weight, add water to decoct and extract, combine the extracts, and concentrate to obtain a Chinese medicine composition extract concentrate; combine the antler velvet extract and the Chinese medicine composition extract concentrate, shake and extract with water-saturated n-butanol, combine the n-butanol solution, wash with ammonia test wave, evaporate the n-butanol solution to dryness, dissolve the residue in dilute ethanol, apply neutral alumina column chromatography, elute with dilute ethanol, collect the eluate, evaporate to dryness, dissolve the residue in water, pass through a D101 macroporous adsorption resin column, elute with water and low concentration ethanol in sequence, discard the eluate, then elute with high concentration ethanol, collect the eluate, evaporate to dryness, dissolve the residue in methanol, and use it as a test solution; (2) Preparation of reference solution Take another astragaloside IV reference substance and add methanol to prepare astragaloside IV reference substance solution; (3) TLC identification The test solution of step (1) and the astragaloside IV reference solution of step (2) were respectively taken and spotted on the same silica gel G thin layer plate, and the lower layer solution of chloroform-methanol-water mixture was used as the developing agent. The plate was developed, taken out, dried, sprayed with sulfuric acid ethanol solution, heated until the spots were clearly colored, and examined under sunlight and ultraviolet light respectively. In the chromatogram of the test sample, at the corresponding position of the chromatogram of the reference sample, spots of the same color appeared under sunlight; fluorescent spots of the same color appeared under ultraviolet light; identification of ginseng reference medicinal materials (1) Taking deer antlers by weight, adding water to decoct and extract to obtain an extract, combining, concentrating, adding ethanol, standing, precipitating, filtering, taking the filtrate, concentrating, and obtaining a deer antler extract; taking ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry by weight, adding water to decoct and extract, combining the extracts, concentrating, and obtaining a concentrated extract of a Chinese medicine composition; combining the deer antler extract and the concentrated extract of the Chinese medicine composition, washing with cyclohexane, and then shaking and extracting with ethyl acetate, combining the ethyl acetate solutions, evaporating to dryness, and dissolving the residue in methanol to prepare a test solution; (2) Take another ginseng control medicinal material, add water to decoct, filter, concentrate the filtrate, shake and extract with n-butanol, combine the n-butanol solution, evaporate to dryness, add methanol to dissolve the residue, and prepare the ginseng control medicinal material solution; (3) The test sample solution and the ginseng control medicinal material solution were aspirated and spotted on the same silica gel G thin layer plate respectively. The plate was developed with ethyl acetate-methanol-pyridine-water for a certain distance. The plate was taken out and dried. The plate was then developed with the upper layer solution of toluene-ethyl acetate-formic acid-water as the developing solvent. The plate was taken out and dried. The plate was sprayed with acetic anhydride-sulfuric acid test solution. In the chromatogram of the test sample, spots of the same color appeared at the corresponding positions in the chromatogram of the control medicinal material.

[0014] As a preferred embodiment, the detection method of the above-mentioned traditional Chinese medicine composition for replenishing qi and nourishing blood comprises qualitative identification by thin layer chromatography and content determination; The said thin layer chromatography qualitative identification includes the identification of astragaloside IV and ginseng as a reference medicinal material; The identification of astragaloside IV includes the following steps: (1) Preparation of test solution Take velvet antler by weight, add 5-20 times the amount of water, decoct and extract 2-4 times, each time for 0.5-3 hours, obtain extract, combine, concentrate to obtain a concentrate with a relative density of 1.2-1.25 at 20°C, add 3 times the amount of ethanol of the concentrate, let it stand for 24-48 hours, precipitate, filter, take the filtrate, concentrate, obtain velvet antler extract, and set aside; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena and wolfberry by weight, add 5-20 times the amount of water, decoct and extract 2-4 times, each time for 0.5-3 hours, combine the decoction extracts, concentrate, obtain a Chinese medicine composition extract concentrate, and set aside; The velvet antler extract and the concentrated extract of the Chinese medicinal composition were extracted by shaking with n-butanol saturated with water for 3 to 4 times, 5 ml each time, and the n-butanol solutions were combined and washed with ammonia test wave for 3 to 4 times, 5 ml each time. The n-butanol solution was evaporated to dryness, and the residue was dissolved in dilute ethanol. The solution was chromatographed on a neutral alumina column and eluted with 50 to 100 ml of 40% ethanol. The eluate was collected and evaporated to dryness. The residue was dissolved in water and passed through a D101 macroporous adsorption resin column, eluted with 20 ml of water and 30 to 90 ml of 40% ethanol in sequence. The eluate was discarded and eluted with 50 to 100 ml of 70% ethanol. The eluate was collected and evaporated to dryness. The residue was dissolved in methanol to serve as the test solution. (2) Preparation of reference solution Take another astragaloside IV reference substance and add methanol to prepare a solution containing 0.5 mg of astragaloside IV reference substance per 1 ml; (3) TLC identification 4 μl of the test solution of step (1) and 5 μl of the astragaloside IV reference solution of step (2) were respectively taken and spotted on the same silica gel G thin layer plate, and the lower layer solution placed below 10°C was used as the developing agent with chloroform-methanol-water in a volume ratio of 13:7:2. The plate was developed, taken out, dried, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under sunlight and ultraviolet light at 365nm. In the chromatogram of the test sample, at the corresponding position of the chromatogram of the reference sample, spots of the same color appeared under sunlight; and fluorescent spots of the same color appeared under ultraviolet light. The identification of ginseng reference medicinal materials includes the following steps: (1) Preparation of test solution Take antler velvet according to weight, add 5 to 20 times of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, obtain extract, combine, concentrate to obtain concentrate with relative density of 1.2 to 1.25 at 20℃, add 3 times of ethanol, let stand for 24 to 48 hours, precipitate, filter, take filtrate, concentrate to obtain antler velvet extract, and set aside; take ginseng, astragalus, yam, lily, donkey hide gelatin, white atractylodes, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides according to weight. and wolfberry, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, combine the decoction extracts, concentrate to obtain the Chinese medicine composition extract concentrate, and set aside; combine the deer antler extract and the Chinese medicine composition extract concentrate, wash with cyclohexane 3 to 4 times, 5 to 10 ml each time, and then shake and extract with ethyl acetate 3 to 4 times, 5 to 10 ml each time, combine the ethyl acetate, evaporate to dryness, and dissolve the residue in 1 ml of methanol to prepare the test solution; (2) Take 1-5 g of ginseng control medicinal material, add 30-150 ml of water, decoct and extract 1-3 times, each time for 1-2 hours, filter, concentrate the filtrate to 10-20 ml, shake and extract with n-butanol 2-4 times, each time 5-10 ml, combine the n-butanol solutions, evaporate to dryness, and dissolve the residue in 1 ml of methanol to prepare the ginseng control medicinal material solution; (3) 4 μl of the test solution and 4 μl of the ginseng control medicinal material solution were respectively spotted on the same silica gel G thin layer plate, and developed with ethyl acetate-methanol-pyridine-water in a volume ratio of 100:17:5:13, and developed with a spread of about 3 cm. The plate was taken out and dried. The upper layer solution of toluene-ethyl acetate-formic acid-water in a volume ratio of 20:10:1:1 was then developed with a spread of about 8 cm. The plate was taken out and dried. The plate was sprayed with acetic anhydride-sulfuric acid solution. In the chromatogram of the test sample, spots of the same color appeared at the corresponding positions in the chromatogram of the control medicinal material.

[0015] As a preferred embodiment, the method for detecting the above-mentioned traditional Chinese medicine composition for invigorating qi and nourishing blood, wherein the content determination comprises the following steps: (1) Preparation of reference solution Take an appropriate amount of loganin reference substance, weigh accurately, and add methanol to make a solution containing 0.1~0.5 mg per 1 ml to obtain loganin (strychnine) reference substance solution; (2) Preparation of test solution Take velvet antler by weight, add 5 to 20 times of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, obtain extract, combine, concentrate to obtain concentrate with relative density of 1.2 to 1.25 at 20℃, add 3 times of ethanol, let stand for 24 to 48 hours, precipitate, filter, take filtrate, concentrate to obtain velvet antler extract, and set aside; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, Add 5 to 20 times the amount of water to Anemarrhena asphodeloides and Lycium barbarum, decoct and extract for 2 to 4 times, each time for 0.5 to 3 hours, combine the decoction extracts, concentrate, and obtain the Chinese medicine composition extract concentrate for later use; combine the deer antler extract and the Chinese medicine composition extract concentrate, accurately measure 3 to 5 ml, place in a 10 ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat for 30 to 50 minutes, cool, add methanol to the scale, shake well, take the supernatant, filter, and take the filtrate; (3) Determination method Accurately aspirate 10 μl of the reference solution and 20 μl of the test solution, respectively, and inject them into a high performance liquid chromatograph, and determine the quantification using the peak area and the external standard one-point method to obtain the product; the high performance liquid chromatography conditions are as follows: octadecylsilane bonded silica gel as the filler; acetonitrile-0.05% phosphoric acid solution in a volume ratio of 22:78 as the mobile phase; the detection wavelength is 236 nm, and the theoretical plate number calculated based on the loganine peak should be not less than 1500.

[0016] The traditional Chinese medicine composition for invigorating qi, nourishing blood and strengthening the body provided by the present invention can be used for conditioning the body's strengthening and fundamental health after radiotherapy and chemotherapy of tumor diseases and surgery, and for treating symptoms such as anemia, thrombocytopenia, and frailty in the elderly caused by adverse reactions of bone marrow suppression.

[0017] The Chinese medicine composition for replenishing qi, nourishing blood and strengthening the body provided by the present invention has a particularly specific formula: In this recipe, ginseng replenishes qi and strengthens the body, regulates immunity, improves fatigue, enhances endurance, promotes lymphocyte proliferation, enhances disease resistance, improves myocardial ischemia, prevents cerebral hypoxic damage, scavenges free radicals, and prolongs cellular telomerase activity. Astragalus is sweet and warm, and enters the spleen and lung meridians. It has the effects of replenishing qi and raising yang, strengthening the body's defenses and strengthening the exterior, expelling toxins and promoting tissue regeneration, and promoting diuresis and reducing swelling. It is used to treat symptoms of spleen and lung qi deficiency and sinking of the middle qi, specifically manifested by fatigue, shortness of breath, laziness, low voice, chills and sweating, loose stools due to spleen deficiency, uterine prolapse, and gastroptosis. It can be used to treat symptoms of qi deficiency and blood deficiency, qi deficiency and blood loss, and qi deficiency and blood stasis. It is compatible with donkey-hide gelatin to replenish qi and promote blood production. Chinese yam has the effects of replenishing the middle qi, strengthening the spleen and lungs, nourishing blood and promoting fluid production, and enhancing immunity. Lily has the effects of nourishing yin and promoting fluid production, moistening the lungs and clearing the heart. Atractylodes macrocephala has the effects of replenishing the spleen and replenishing qi, treating spleen and stomach qi deficiency, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. Cornus officinalis nourishes the liver and kidneys, astringes essence and strengthens deficiency, promotes fluid production and quenches thirst, has anti-inflammatory properties, and lowers blood pressure and blood sugar. Ligustrum lucidum nourishes the liver and kidneys, improves essence and blood, blackens hair, strengthens tendons and bones, and clears turbidity and reduces lipids. Schisandra chinensis has astringent and firming properties, invigorates qi and promotes fluid production, and nourishes the kidneys and calms the mind. It can treat chronic cough, asthma, spontaneous perspiration, night sweats, spermatorrhea and spermatorrhea caused by kidney deficiency and weak essence, chronic diarrhea caused by spleen and kidney deficiency and cold, heat damage to yin, excessive sweating and thirst. Tangerine peel regulates qi and strengthens the spleen, dries dampness and resolves phlegm. The volatile oils in tangerine peel have a mild stimulating effect on the gastrointestinal tract, dispelling wind and purging qi to a certain extent. They effectively promote the secretion of digestive juices and gastric acid, accelerate gastrointestinal motility, and facilitate the elimination of intestinal gas. The flavonoids in tangerine peel have cardiovascular protective effects. Anemarrhena asphodeloides has heat-clearing and blood-cooling properties. It treats consumptive conditions such as hot flashes and night sweats, lung-heat-induced cough and asthma, hematemesis, epistaxis, hematuria, diabetes, and hypertension. Lycium barbarum has the benefits of tonifying the kidneys and enhancing yang, strengthening bones and tendons, improving cardiovascular and cerebrovascular function, inhibiting osteoporosis, enhancing immunity, fighting tumors, combating fatigue, protecting cardiovascular health, and improving osteoporosis. The entire formula strengthens the spleen and qi, tonifies the liver and kidneys, and collectively invigorates qi, nourishes blood, and strengthens immunity.

[0018] Beneficial effects: The present invention has the following technical advantages: 1. The present invention is based on the theory of traditional Chinese medicine and adopts the theory of syndrome differentiation and treatment to screen the prescription. The combination of each medicinal flavor is scientific and reasonable, and together they play the role of replenishing qi and nourishing blood, consolidating the foundation and strengthening the body. It is used for shortness of breath, palpitations, sallow complexion, and physical weakness caused by qi and blood deficiency. In addition, it can improve the body immunity of patients with qi and blood deficiency, and can also be used to reduce adverse reactions such as bone marrow suppression and thrombocytopenia caused by radiotherapy and chemotherapy.

[0019] 2. The qualitative identification and content determination methods provided by the present invention, based on the specific active ingredients of a traditional Chinese medicine composition, have been optimized through extensive experimental screening to develop methods for identifying astragaloside IV and a control herb, ginseng. Furthermore, a high-performance liquid chromatography (HPLC) assay for loganin, including chromatographic conditions such as mobile phase composition, has been developed through experimental screening. This method allows for comprehensive and objective qualitative identification and content determination of active ingredients in traditional Chinese medicine compositions, which is of great significance for ensuring their clinical efficacy. DETAILED DESCRIPTION

[0020] The present invention is further illustrated below with reference to specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention made by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0021] Example 1 1. A Chinese medicinal composition for invigorating qi and nourishing blood, which is prepared from the following Chinese medicinal raw materials in parts by weight: 10g ginseng, 85g astragalus, 75g yam, 50g lily, 35g donkey-hide gelatin, 35g stir-fried atractylodes, 35g cornus fruit, 35g privet, 25g schisandra, 35g tangerine peel, 25g anemarrhena, 2g deer antler, 50g wolfberry.

[0022] 2. A method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood, comprising the following steps: (1) Take 2g of velvet antler by weight, add 20 times the amount of water, decoct and extract three times, the first two times for 3 hours, the third time for 1 hour, combine the three extracts, concentrate to obtain a concentrate with a relative density of 1.2-1.25 at 20°C, add 3 times the amount of ethanol of the concentrate, let it stand for 24 hours, precipitate, filter, take the filtrate, concentrate, and obtain velvet antler extract for later use; (2) Take 10g of ginseng, 85g of astragalus, 75g of yam, 50g of lily, 35g of donkey-hide gelatin, 35g of stir-fried atractylodes, 35g of cornus fruit, 30g of privet, 25g of schisandra, 35g of dried tangerine peel, 25g of anemarrhena, and 50g of wolfberry according to weight, add 10 times the amount of water, decoct and extract twice, the first time for 3 hours and the second time for 2 hours, combine the extracts from the two decoctions, concentrate, and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing an oral solution.

[0023] 3. A method for detecting a traditional Chinese medicine composition for invigorating qi and nourishing blood, comprising qualitative identification by thin layer chromatography and content determination; The said thin layer chromatography qualitative identification includes the identification of astragaloside IV and ginseng as a reference medicinal material; The identification of astragaloside IV includes the following steps: (1) Preparation of test solution Take 10 ml of the oral solution prepared above, shake and extract it with n-butanol saturated with water 3 times, 5 ml each time, combine the n-butanol solutions, wash with ammonia test wave 3 times, 5 ml each time, evaporate the n-butanol solution to dryness, dissolve the residue in 1 ml of dilute ethanol, add it to a neutral alumina column (100-120 mesh, 2 g, inner diameter 1-1.5 cm), elute with 50 ml of 40% ethanol, collect the eluate, evaporate to dryness, dissolve the residue in 1 ml of water, pass it through a D101 macroporous adsorption resin column (inner diameter 1.2-1.5 cm, column height 12 cm), elute with 20 ml of water and 30 ml of 40% ethanol in sequence, discard the eluate, and elute with 50 ml of 70% ethanol, collect the eluate, evaporate to dryness, dissolve the residue in 1 ml of methanol as the test solution; (2) Preparation of reference solution Take another astragaloside IV reference substance and add methanol to prepare a solution containing 0.5 mg of astragaloside IV reference substance per 1 ml; (3) TLC identification 4 μl of the test solution of step (1) and 5 μl of the astragaloside IV reference solution of step (2) were respectively taken and spotted on the same silica gel G thin layer plate, and the lower layer solution placed below 10°C was used as the developing agent with chloroform-methanol-water in a volume ratio of 13:7:2. The plate was developed, taken out, dried, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under sunlight and ultraviolet light at 365nm. In the chromatogram of the test sample, at the corresponding position of the chromatogram of the reference sample, spots of the same color appeared under sunlight; and fluorescent spots of the same color appeared under ultraviolet light. The identification of ginseng reference medicinal materials includes the following steps: (1) Preparation of test solution Take 10 ml of the oral solution prepared above, shake and extract with n-butanol three times, 5 ml each time, combine the n-butanol solutions, evaporate to dryness, and dissolve the residue in 1 ml of methanol to prepare the test solution; (2) Take 1 g of ginseng control medicinal material, add 30 ml of water, decoct for 1 hour, filter, concentrate the filtrate to 10 ml, shake and extract with n-butanol 3 times, 5 ml each time, combine the n-butanol solutions, evaporate to dryness, add 1 ml of methanol to dissolve the residue, and prepare the ginseng control medicinal material solution; (3) 4 μl of the test solution and 4 μl of the ginseng control medicinal material solution were respectively spotted on the same silica gel G thin layer plate, and developed with ethyl acetate-methanol-pyridine-water in a volume ratio of 100:17:5:13, and developed with a spread of about 3 cm. The plate was taken out and dried. The upper layer solution of toluene-ethyl acetate-formic acid-water in a volume ratio of 20:10:1:1 was then developed with a spread of about 8 cm. The plate was taken out and dried, and sprayed with acetic anhydride-sulfuric acid test solution. In the chromatogram of the test sample, spots of the same color appeared at the corresponding positions in the chromatogram of the control medicinal material.

[0024] The assay includes the following steps: (1) Preparation of reference solution Take an appropriate amount of loganin reference substance, accurately weigh it, and add methanol to make a solution containing 0.1 mg per 1 ml to obtain the loganin reference substance solution; (2) Preparation of test solution Take 3 ml of the oral solution prepared above, place it in a 10 ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat for 30 minutes, let it cool, add methanol to the scale, shake well, take the supernatant, filter, and take the filtrate; (3) Determination method Accurately pipette 10 μl of the loganin reference solution and 20 μl of the test solution into a high-performance liquid chromatograph. The loganin content in the test sample is determined qualitatively based on the retention time of the loganin reference solution and quantitatively based on the peak area using the external standard single-point method. This product contains 0.16 mg of loganin per 1 ml of wolfberry. The HPLC conditions are as follows: octadecylsilane bonded silica gel as the filler; acetonitrile-0.05% phosphoric acid solution in a volume ratio of 22:78 as the mobile phase; a detection wavelength of 236 nm; and a theoretical plate number of no less than 1500, calculated based on the loganin peak.

[0025] Example 2 1. A Chinese medicinal composition for invigorating qi and nourishing blood, which is prepared from the following Chinese medicinal raw materials in parts by weight: Ginseng 16g, Astragalus 160g, Chinese yam 150g, Lily 100g, Donkey-hide gelatin 60g, Stir-fried Atractylodes 60g, Cornus officinalis 60g, Ligustrum lucidum 60g, Schisandra 50g, Tangerine peel 60g, Anemarrhena 50g, Deer antler 3.0g, Lycium barbarum 100g.

[0026] 2. A method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood, comprising the following steps: (1) Take 3g of velvet antler by weight, add 20 times the amount of water, decoct and extract twice, each time for 3 hours, combine the two extracts, concentrate to obtain a concentrate with a relative density of 1.2-1.25 at 20°C, add 3 times the amount of ethanol, let it stand for 24 hours, precipitate, filter, take the filtrate, concentrate, and obtain velvet antler extract for later use; (2) Take 16g of ginseng, 160g of astragalus, 150g of yam, 100g of lily, 60g of donkey-hide gelatin, 60g of stir-fried atractylodes, 60g of cornus fruit, 60g of privet, 50g of schisandra, 60g of dried tangerine peel, 50g of anemarrhena, and 100g of wolfberry according to weight, add 15 times the amount of water, decoct and extract three times, the first two times for 2 hours, the third time for 1 hour, combine the three decoction extracts, concentrate, and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing tablets.

[0027] 3. The thin layer chromatography identification and content determination methods are the same as in Example 1.

[0028] Example 3 1. A Chinese medicinal composition for invigorating qi and nourishing blood, which is prepared from the following Chinese medicinal raw materials in parts by weight: 24g ginseng, 240g astragalus, 225g yam, 150g lily, 90g donkey-hide gelatin, 90g stir-fried atractylodes, 90g cornus officinalis, 90g privet, 75g schisandra, 90g tangerine peel, 75g anemarrhena, 4.5g deer antler, 150g wolfberry.

[0029] 2. A method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood, comprising the following steps: (1) Take 4.5 g of velvet antler by weight, add 15 times the amount of water, decoct and extract twice, each time for 2 hours, combine the two extracts, concentrate to obtain a concentrate with a relative density of 1.2-1.25 at 20°C, add 4 times the amount of ethanol, let it stand for 48 hours, precipitate, filter, take the filtrate, concentrate, and obtain velvet antler extract for later use; (2) Take 24g of ginseng, 240g of astragalus, 225g of yam, 150g of lily, 90g of donkey-hide gelatin, 90g of stir-fried atractylodes, 90g of cornus fruit, 90g of privet, 75g of schisandra, 90g of dried tangerine peel, 75g of anemarrhena, and 150g of wolfberry according to weight, add 12 times the amount of water, decoct and extract twice, each time for 2 hours, combine the extracts from the two decoctions, concentrate, and set aside; (3) The velvet antler extract obtained in step (1) and the extract obtained in step (2) are combined, and a pharmaceutically acceptable carrier is added to prepare granules.

[0030] 3. The thin layer chromatography identification and content determination methods are the same as in Example 1.

[0031] Example 4 Functional Experiment 1. Qi and blood tonification experiment (acetylphenylhydrazine method) Forty male rats were randomly divided into three groups: a normal saline group and a low-dose and high-dose group of the oral solution prepared in Example 1. All groups were administered by gavage. Before administration, blood was collected from the tail to measure the Hb R content, RBC, WBC, and Heiden's body counts. On the first, fourth, and seventh days of administration, a subcutaneous injection of 2% acetylphenylhydrazine in normal saline was administered once each, with the first dose being 1 ml / 100 g, and the dose being halved each subsequent time. On the tenth day of administration, blood was collected from the tail to repeat the above-mentioned measurements. The specific experimental results are shown in Table 1.

[0032] Table 1 Effects of Chinese herbal medicine combination on hematological parameters of rats with blood and qi deficiency syndrome X̄±SD Group dose Number of animals Hb (g / %) RBC (10,000 / mm³) WBC (10,000 / mm³) Heidegger bodies Normal saline group — 10 4.23±1.25 214±13 29±2.1 18±2.9 Example 1 Oral Liquid Low Dose Group 0.2ml / 10g 10 9.56±2.75* 459±15* 17±3.0* 12±2.4* Example 1 Oral Liquid High Dose Group 0.4ml / 10g 10 11.27±2.01** 480±16** 11±2.2** 9±2.1** *P<0.05, **P<0.01.

[0033] As shown in Table 1, the Hb content of the animals in the group receiving the Qi-invigorating and blood-nourishing Chinese herbal composition prepared in Example 1 was significantly increased, the RBC count increased, and the WBC and Heiden's body counts decreased. Furthermore, the animals' symptoms of Qi and blood deficiency, such as loose and dull hair and decreased activity, were also improved. This indicates that the Qi-invigorating and blood-nourishing Chinese herbal composition prepared in Example 1 has a significant effect of replenishing Qi and blood.

[0034] 2. Blood circulation and blood stasis removal effect Healthy SD rats, half male and half female, weighing 260±12g, were randomly divided into four groups: a normal saline group, a low-dose and high-dose oral solution prepared in Example 1, and a scopolamine group. All groups were administered by gavage. Anisodamine injection served as a positive control. The animals in each experimental group were fasted for 24 hours under experimental conditions. Anesthesia was achieved by intraperitoneal injection of 50mg / kg sodium pentobarbital. A jejunal loop was removed from the right abdominal wall and placed in a constant-temperature perfusion tank. Rockwell's solution at approximately 37°C was perfused, and the drug solution was dripped into the perfusion tank. After 10 minutes, the effects of the drug on microcirculatory vessel diameter, flow velocity, flow pattern, and the number of open capillaries within a 1mm² visual field were observed using a 40-80x microscope. 0.01 ml of normal saline was added to the normal saline group, 1 ml of the original solution (high concentration group) and 50% dilution (low concentration group) of the traditional Chinese medicine composition for invigorating qi and nourishing blood prepared in Example 1 was added dropwise, and 0.01 ml of the anisodamine positive control group was added dropwise. The specific experimental results are shown in Table 2.

[0035] Table 2 Effects of the traditional Chinese medicine composition for replenishing qi and nourishing blood on rat mesenteric microcirculation X̄±SD *P < 0.05, **P < 0.01; The results in Table 2 show that the diameter of the mesenteric microvessels and the microblood flow velocity of the animals in the group of the traditional Chinese medicine composition for invigorating qi and nourishing blood prepared in Example 1 were significantly increased, the number of open capillaries in the 1 mm² visual area was significantly increased, and the blood flow pattern was changed from being mainly granular flow to being mainly linear flow, indicating that the traditional Chinese medicine composition for invigorating qi and nourishing blood of the present invention has the effects of significantly improving microcirculation, increasing local blood flow, and promoting blood circulation and removing blood stasis.

[0036] 3. Impact on the immune system Immune cell antibody secretion test: 30 ICR mice, half male and half female, weighing 27±2g, were randomly divided into three groups: a normal saline group, a low-dose group of the oral solution of Example 1, and a high-dose group. Each group was administered by gavage for seven consecutive days. Each group was then intraperitoneally injected with 0.2ml of a 5% sheep red blood cell suspension. Four days later, the mice were decapitated and their spleens were removed. A spleen cell suspension of 15mg spleen / ml was prepared in PBS. The spleen cell suspension, 0.2% sheep red blood cells, and 0.5ml of 1:10 guinea pig serum were then added to test tubes. The tubes were incubated in a 37°C water bath for 1 hour and centrifuged at 400 rpm for 10 minutes. The supernatant was collected and spectrophotometrically analyzed at 415nm. The OD values of each group were recorded. The specific experimental results are shown in Table 3.

[0037] Table 3 Effects of Chinese herbal medicine oral solution on cellular immune function in mice (X̄±SD) Group Number of animals dose OD value Improvement rate (%) P-value Normal saline group 10 — 0.40 0.00 Low-dose oral solution group 10 0.4ml / 10g 0.52 30.00 <0.05 High-dose oral solution group 10 0.8ml / 10g 0.60 50.00 <0.01

[0038] The experimental results in Table 3 show that the OD values of the animals in both the high-dose and low-dose groups of the Chinese herbal composition oral liquid were significantly improved. This indicates that the Chinese herbal composition oral liquid significantly improves the antibody production capacity of the mice's immune cells and antibody-forming cells, demonstrating a relatively good effect in improving immunity.

Claims

1. A Chinese medicine composition for invigorating qi and nourishing blood, characterized in that: It includes the following Chinese medicinal materials: Ginseng, astragalus, yam, lily, donkey-hide gelatin, stir-fried Atractylodes macrocephala, cornus officinalis, privet, Schisandra chinensis, tangerine peel, Anemarrhena asphodeloides, deer antler, and wolfberry.

2. A Chinese medicine composition for invigorating qi and nourishing blood according to claim 1, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in parts by weight: 8-24 parts of ginseng, 80-240 parts of astragalus, 75-225 parts of yam, 50-150 parts of lily, 30-90 parts of donkey-hide gelatin, 30-90 parts of stir-fried atractylodes, 30-90 parts of cornus fruit, 30-90 parts of privet, 25-75 parts of schisandra, 30-90 parts of dried tangerine peel, 25-75 parts of anemarrhena, 1.5-4.5 parts of deer antler, and 50-150 parts of wolfberry.

3. A Chinese medicine composition for invigorating qi and nourishing blood according to claim 2, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in parts by weight: 8-16 parts of ginseng, 80-160 parts of astragalus, 75-150 parts of yam, 50-100 parts of lily, 30-60 parts of donkey-hide gelatin, 30-60 parts of stir-fried atractylodes, 30-60 parts of cornus fruit, 30-60 parts of privet, 25-50 parts of schisandra, 30-60 parts of dried tangerine peel, 25-50 parts of anemarrhena, 1.5-3.0 parts of deer antler, and 50-100 parts of wolfberry.

4. A traditional Chinese medicine composition for invigorating qi and nourishing blood according to claim 3, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in parts by weight: 10 parts of ginseng, 85 parts of astragalus, 75 parts of yam, 50 parts of lily, 35 parts of donkey-hide gelatin, 35 parts of stir-fried atractylodes, 35 parts of cornus fruit, 30 parts of privet, 25 parts of schisandra, 35 parts of dried tangerine peel, 25 parts of anemarrhena, 2 parts of deer antler, and 50 parts of wolfberry.

5. A method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Deer antlers are boiled in water according to weight to obtain an extract, which is then combined, concentrated, and ethanol is added. The mixture is allowed to stand for precipitation, filtered, and the filtrate is concentrated to obtain a deer antler extract for later use; (2) Take ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry according to weight, add water and boil to extract, combine the extracts, concentrate, and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing a traditional Chinese medicine preparation.

6. The method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood according to claim 5, characterized in that: The following steps are involved: (1) Take velvet antler by weight, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, to obtain the extract, combine and concentrate to obtain a concentrate with a relative density of 1.2 to 1.25 at 20°C, add 3 times the amount of ethanol to the concentrate, let it stand for 24 to 48 hours, precipitate, filter, take the filtrate, concentrate, and obtain the velvet antler extract for later use; (2) Take ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, white atractylodes, cornus fruit, privet, schisandra chinensis, dried tangerine peel, anemarrhena asphodeloides and wolfberry according to their weight, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, combine the decoction extracts, concentrate and set aside; (3) Combining the velvet antler extract from step (1) and the extract from step (2), adding a pharmaceutically acceptable carrier, and preparing a traditional Chinese medicine preparation.

7. The method for preparing a traditional Chinese medicine composition for invigorating qi and nourishing blood according to claim 5, characterized in that: The Chinese medicine preparations include oral liquid, tablets, capsules, granules, pills, mixtures, powders or extracts.

8. The method for detecting the qi-invigorating and blood-nourishing Chinese medicine composition according to any one of claims 1 to 4, characterized in that: Including thin layer chromatography qualitative identification and content determination; The said thin layer chromatography qualitative identification includes the identification of astragaloside IV and ginseng as a reference medicinal material; The identification of astragaloside IV includes the following steps: (1) Preparation of test solution Take antler velvet by weight, add water to decoct and extract to obtain an extract, combine, concentrate, add ethanol, let stand, precipitate, filter, take the filtrate, concentrate, and obtain antler velvet extract; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry by weight, add water to decoct and extract, combine the extracts, and concentrate to obtain a Chinese medicine composition extract concentrate; combine the antler velvet extract and the Chinese medicine composition extract concentrate, shake and extract with water-saturated n-butanol, combine the n-butanol solution, wash with ammonia test wave, evaporate the n-butanol solution to dryness, dissolve the residue in dilute ethanol, apply neutral alumina column chromatography, elute with dilute ethanol, collect the eluate, evaporate to dryness, dissolve the residue in water, pass through a D101 macroporous adsorption resin column, elute with water and low concentration ethanol in sequence, discard the eluate, then elute with high concentration ethanol, collect the eluate, evaporate to dryness, dissolve the residue in methanol, and use it as a test solution; (2) Preparation of reference solution Take another astragaloside IV reference substance and add methanol to prepare astragaloside IV reference substance solution; (3) TLC identification The test solution of step (1) and the astragaloside IV reference solution of step (2) were respectively taken and spotted on the same silica gel G thin layer plate, and the lower layer solution of chloroform-methanol-water mixture was used as the developing agent. The plate was developed, taken out, dried, sprayed with sulfuric acid ethanol solution, and heated until the spots were clearly colored. The plates were placed under sunlight and ultraviolet light for inspection. In the chromatogram of the test sample, at the corresponding position of the chromatogram of the reference sample, spots of the same color appeared under sunlight; and fluorescent spots of the same color appeared under ultraviolet light. Identification of Ginseng Reference Herbs (1) Taking deer antlers by weight, adding water to decoct and extract to obtain an extract, combining, concentrating, adding ethanol, standing, precipitating, filtering, taking the filtrate, concentrating, and obtaining a deer antler extract; taking ginseng, astragalus, Chinese yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides, and wolfberry by weight, adding water to decoct and extract, combining the extracts, concentrating, and obtaining a concentrated extract of a Chinese medicine composition; combining the deer antler extract and the concentrated extract of the Chinese medicine composition, washing with cyclohexane, and then shaking and extracting with ethyl acetate, combining the ethyl acetate solutions, evaporating to dryness, and dissolving the residue in methanol to prepare a test solution; (2) Take another ginseng control medicinal material, add water to decoct, filter, concentrate the filtrate, shake and extract with n-butanol, combine the n-butanol solution, evaporate to dryness, add methanol to dissolve the residue, and prepare the ginseng control medicinal material solution; (3) The test sample solution and the ginseng control medicinal material solution were aspirated and spotted on the same silica gel G thin layer plate respectively. The plate was developed with ethyl acetate-methanol-pyridine-water for a certain distance. The plate was taken out and dried. The plate was then developed with the upper layer solution of toluene-ethyl acetate-formic acid-water as the developing solvent. The plate was taken out and dried. The plate was sprayed with acetic anhydride-sulfuric acid test solution. In the chromatogram of the test sample, spots of the same color appeared at the corresponding positions in the chromatogram of the control medicinal material.

9. The method for detecting a traditional Chinese medicine composition for invigorating qi and nourishing blood according to claim 8, characterized in that: Including thin layer chromatography qualitative identification and content determination; The said thin layer chromatography qualitative identification includes the identification of astragaloside IV and ginseng as a reference medicinal material; The identification of astragaloside IV includes the following steps: (1) Preparation of test solution Take velvet antler by weight, add 5-20 times the amount of water, decoct and extract 2-4 times, each time for 0.5-3 hours, obtain extract, combine, concentrate to obtain a concentrate with a relative density of 1.2-1.25 at 20°C, add 3 times the amount of ethanol of the concentrate, let it stand for 24-48 hours, precipitate, filter, take the filtrate, concentrate, obtain velvet antler extract, and set aside; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena and wolfberry by weight, add 5-20 times the amount of water, decoct and extract 2-4 times, each time for 0.5-3 hours, combine the decoction extracts, concentrate, obtain a Chinese medicine composition extract concentrate, and set aside; The velvet antler extract and the concentrated extract of the Chinese medicinal composition were extracted by shaking with n-butanol saturated with water for 3 to 4 times, 5 ml each time, and the n-butanol solutions were combined and washed with ammonia test wave for 3 to 4 times, 5 ml each time. The n-butanol solution was evaporated to dryness, and the residue was dissolved in dilute ethanol. The solution was chromatographed on a neutral alumina column and eluted with 50 to 100 ml of 40% ethanol. The eluate was collected and evaporated to dryness. The residue was dissolved in water and passed through a D101 macroporous adsorption resin column, eluted with 20 ml of water and 30 to 90 ml of 40% ethanol in sequence. The eluate was discarded and eluted with 50 to 100 ml of 70% ethanol. The eluate was collected and evaporated to dryness. The residue was dissolved in methanol to serve as the test solution. (2) Preparation of reference solution Take another astragaloside IV reference substance and add methanol to prepare a solution containing 0.5 mg of astragaloside IV reference substance per 1 ml; (3) TLC identification 4 μl of the test solution of step (1) and 5 μl of the astragaloside IV reference solution of step (2) were respectively taken and spotted on the same silica gel G thin layer plate, and the lower layer solution placed below 10°C was used as the developing agent with chloroform-methanol-water in a volume ratio of 13:7:

2. The plate was developed, taken out, dried, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under sunlight and ultraviolet light at 365nm. In the chromatogram of the test sample, at the corresponding position of the chromatogram of the reference sample, spots of the same color appeared under sunlight; and fluorescent spots of the same color appeared under ultraviolet light. The identification of ginseng reference herbs includes the following steps: (1) Preparation of test solution Take antler velvet according to weight, add 5 to 20 times of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, obtain extract, combine, concentrate to obtain concentrate with relative density of 1.2 to 1.25 at 20℃, add 3 times of ethanol, let stand for 24 to 48 hours, precipitate, filter, take filtrate, concentrate to obtain antler velvet extract, and set aside; take ginseng, astragalus, yam, lily, donkey hide gelatin, white atractylodes, cornus fruit, privet, schisandra chinensis, tangerine peel, anemarrhena asphodeloides according to weight. and wolfberry, add 5 to 20 times the amount of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, combine the decoction extracts, concentrate to obtain the Chinese medicine composition extract concentrate, and set aside; combine the deer antler extract and the Chinese medicine composition extract concentrate, wash with cyclohexane 3 to 4 times, 5 to 10 ml each time, and then shake and extract with ethyl acetate 3 to 4 times, 5 to 10 ml each time, combine the ethyl acetate, evaporate to dryness, and dissolve the residue in 1 ml of methanol to prepare the test solution; (2) Take 1-5 g of ginseng control medicinal material, add 30-150 ml of water, decoct and extract 1-3 times, each time for 1-2 hours, filter, concentrate the filtrate to 10-20 ml, shake and extract with n-butanol 2-4 times, each time 5-10 ml, combine the n-butanol solutions, evaporate to dryness, and dissolve the residue in 1 ml of methanol to prepare the ginseng control medicinal material solution; (3) 4 μl of the test solution and 4 μl of the ginseng control medicinal material solution were respectively spotted on the same silica gel G thin layer plate, and developed with ethyl acetate-methanol-pyridine-water in a volume ratio of 100:17:5:13, and developed with a spread of about 3 cm. The plate was taken out and dried. The upper layer solution of toluene-ethyl acetate-formic acid-water in a volume ratio of 20:10:1:1 was then developed with a spread of about 8 cm. The plate was taken out and dried. The plate was sprayed with acetic anhydride-sulfuric acid solution. In the chromatogram of the test sample, spots of the same color appeared at the corresponding positions in the chromatogram of the control medicinal material.

10. The method for detecting a traditional Chinese medicine composition for invigorating qi and nourishing blood according to claim 8, characterized in that: The assay includes the following steps: (1) Preparation of reference solution Take an appropriate amount of loganin reference substance, accurately weigh it, and add methanol to make a solution containing 0.1-0.5 mg per 1 ml to obtain the loganin reference substance solution; (2) Preparation of test solution Take antler velvet according to weight, add 5 to 20 times of water, decoct and extract 2 to 4 times, each time for 0.5 to 3 hours, obtain extract, combine, concentrate to obtain concentrate with relative density of 1.2 to 1.25 at 20℃, add 3 times of ethanol, let stand for 24 to 48 hours, precipitate, filter, take filtrate, concentrate to obtain antler velvet extract, and set aside; take ginseng, astragalus, yam, lily, donkey-hide gelatin, atractylodes macrocephala, cornus fruit, privet, schisandra chinensis, Add 5-20 times the amount of water to dried tangerine peel, rhizoma anemarrhenae, and wolfberry, decoct and extract 2-4 times, each time for 0.5-3 hours, combine the decoction extracts, concentrate to obtain a Chinese medicine composition extract concentrate, and set aside; combine the antler extract and the Chinese medicine composition extract concentrate, accurately measure 3-5 ml, place in a 10 ml volumetric flask, add an appropriate amount of methanol, ultrasonically treat for 30-50 minutes, let cool, add methanol to the scale, shake well, take the supernatant, filter, and take the filtrate to obtain; (3) Determination method Accurately pipette 10 μl of the reference solution and 20 μl of the test solution respectively, inject them into the high performance liquid chromatograph, and determine the quantification by the peak area and the external standard one-point method; The high performance liquid chromatography conditions are as follows: octadecylsilane bonded silica gel as a filler; acetonitrile-0.05% phosphoric acid solution in a volume ratio of 22:78 as a mobile phase; a detection wavelength of 236 nm, and a theoretical plate number calculated based on the loganine peak that should be no less than 1500.

11. Use of the Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a medicine for invigorating qi and nourishing blood.

12. Use of the traditional Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a medicament for treating myelosuppression induced by radiotherapy and chemotherapy.

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