Perfusion device for stem cell culture

Abstract

The invention discloses a perfusion device for stem cell culture, and belongs to the technical field of cell culture.The perfusion device comprises a frame, a closing assembly convenient to overhaul is arranged on the frame, a culture container is arranged in the frame, a perfusion bottle is fixedly installed in the frame, a filter box is fixedly installed in the frame, and the filter box is connected with the perfusion bottle. A pumping assembly is arranged in the frame, a penetrating groove is formed in the top of the filter box, and a connecting pipe is connected to the filter box; culture cells are conveniently collected through the arranged filter cylinder, the cells cultured in the perfusion bottle and a culture solution flow into the filter box through a liquid supply pipe, the filter cylinder is covered with a filter membrane, the liquid can flow to the outside to intercept the cells, then the cells can be filtered in the filter cylinder to be collected, then the filter cylinder is rotated, and the cells can be conveniently collected. The threaded sleeve is separated from the threaded head, so that the filter cartridge can be detached, the cells can be conveniently separated and collected, and the working efficiency is improved.

Description

Technical Field

[0001] The present invention specifically relates to a perfusion device for stem cell culture, and belongs to the technical field of cell culture. Background Art

[0002] The perfusion device used for stem cell culture is mainly used to provide the nutrients required for cell growth and effectively remove waste generated by cell metabolism. Through the perfusion system, various growth factors and nutrients can be continuously provided to ensure that stem cells obtain sufficient nutrients during the culture process. Cells will produce waste during proliferation and metabolism. The perfusion device can discharge these wastes in time to avoid their accumulation and toxicity to the cells. It can maintain the uniformity and stability of the cell culture fluid and help create a more ideal cell growth microenvironment.

[0003] Patent document CN213086004U discloses a perfusion device for stem cell culture, "including a culture dish, a perfusion bottle, a collection tray and a waste liquid bottle: the culture dish is arranged on one side of the perfusion bottle and is connected to the perfusion bottle through an input tube; one end of the input tube extends into the culture dish, and the other end extends into the perfusion bottle; a box is installed above the perfusion bottle; a motor is provided in the box; an electric telescopic rod is connected to the bottom of the motor; the bottom of the electric telescopic rod is connected to a scraper arranged in the perfusion bottle; a control panel is also provided on the top surface of the box; an output port is also provided at the bottom of the perfusion bottle; one end of the output tube is connected to the output port, and the other end is connected to the collection tray; the waste liquid bottle is arranged on the other side of the perfusion bottle; one end of the waste liquid tube extends into the collection tray, and the other end extends into the waste liquid bottle. The utility model proposes a perfusion device for stem cell culture, which has a simple structure, is easy to clean, and can prevent stem cells from remaining in the perfusion bottle."

[0004] However, existing perfusion devices for stem cell culture have some limitations in terms of cell collection. When the cultured cells need to be recovered, additional tools and equipment are usually required to intercept and collect the cells. This process is not only cumbersome but also directly affects work efficiency. In the process of collecting cells, operators often need to use specialized tools. These additional operating steps increase the complexity of the workflow, especially in experiments that require frequent cell collection, which more significantly affects overall efficiency. Summary of the Invention

[0005] The purpose of the present invention is to address the deficiencies of the prior art and provide a perfusion device for stem cell culture to facilitate the collection of cultured cells.

[0006] A perfusion device for stem cell culture comprises a frame, the frame is provided with a closing component for easy maintenance, a culture container is provided inside the frame, a perfusion bottle is fixedly installed inside the frame, a filter box is fixedly installed inside the frame, a pumping component is provided inside the frame, a through groove is provided on the top of the filter box, a connecting pipe is connected to the filter box, a square groove is passed through the through groove, a square frame is fixedly connected to the square groove, a cleaning component is provided inside the square groove, a sealing ring is connected to both the square groove and the square frame, a support plate is fixedly installed inside the square groove, and a filter component for cell separation is provided on the support plate.

[0007] Furthermore, the closing assembly includes an inspection port and a sealing door, wherein the inspection port is provided on a side of the frame, and the sealing door is mounted on the frame via a hinge, and the sealing door is used to seal the inspection port.

[0008] Furthermore, a No. 1 connector and a No. 2 connector are installed on the frame, and the No. 1 connector and the No. 2 connector are symmetrically arranged. A No. 1 pipe and a No. 2 pipe are fixedly installed on the top of the perfusion bottle, and one end of the No. 2 pipe is connected to the No. 1 connector, the No. 1 pipe is connected to the bottom of the culture container, and the No. 2 connector is connected to the culture container.

[0009] Furthermore, drainage tubes are symmetrically arranged at the bottom of the perfusion bottle, one of the drainage tubes is connected to a waste liquid tube, and one end of the waste liquid tube extends out of the frame, and the other drainage tube is connected to a liquid supply tube.

[0010] Furthermore, the No. 1 pipe, the No. 2 pipe, the waste liquid pipe and the liquid supply pipe are all provided with electromagnetic valves for controlling opening and closing, and a handle is fixedly installed on the top of the square groove.

[0011] Furthermore, the filter assembly includes a threaded head, a threaded sleeve and a filter cartridge. The threaded head array is installed on the square groove, the threaded sleeve is threadedly connected to the corresponding threaded head, and the filter cartridge is fixedly connected to the threaded sleeve.

[0012] Furthermore, the pumping assembly includes a pumping pump and a discharge pipe. The pumping pump is fixedly installed inside the frame, and the input end of the pumping pump is connected to the connecting pipe. One end of the discharge pipe is connected to the output end of the pumping pump, and one end of the discharge pipe extends out of the frame.

[0013] Furthermore, the cleaning assembly includes a driving mechanism, a guide mechanism, a strip plate, a receiving groove and a scraper. The driving mechanism for driving the strip plate to move is arranged on the square groove, and the guide mechanism for guiding the strip plate is arranged inside the square groove. The receiving groove is arranged on the strip plate, and the scraper is fixedly installed on the strip plate, and the scraper is in contact with the side of the support plate, and the scraper is arranged at an angle.

[0014] Furthermore, the driving mechanism includes a power supply battery, a control button, a driving motor, a threaded screw, a connecting block and a threaded ring. Sliding grooves are symmetrically arranged inside the square groove, and the threaded screw is arranged inside one of the sliding grooves. The connecting block is fixedly connected to one end of the strip plate, and the threaded ring is fixedly arranged inside the connecting block, and the threaded screw is threadedly connected to the threaded ring. The driving motor is fixedly arranged at the top of the square groove, and one end of the threaded screw is fixedly connected to the output end of the driving motor. The power supply battery is fixedly installed on the top of the square frame, and the power supply battery is electrically connected to the driving motor. A control button is provided on the power supply battery, and the control button is electrically connected to the driving motor.

[0015] Furthermore, the guide mechanism includes a guide rod and a guide block, the guide block is fixedly mounted on the strip plate, the guide rod is fixedly mounted inside another sliding groove, the guide block is fixedly mounted at one end of the strip plate, and the guide rod passes through the guide block.

[0016] Beneficial effects:

[0017] 1. The present invention facilitates the collection of cultured cells by providing a filter cartridge. The cells and culture fluid cultured in the perfusion bottle flow into the filter box through the liquid supply tube. The filter cartridge is covered with a filter membrane, which allows the liquid to flow to the outside and intercept the cells, thereby filtering the cells inside the filter cartridge for collection. Pulling the handle can drive the square groove to move, thereby driving the filtered cells to move to the outside. Then, rotating the filter cartridge drives the threaded sleeve to rotate, thereby separating the threaded sleeve from the threaded head, and then the filter cartridge can be disassembled, which facilitates the separation and collection of cells and improves work efficiency.

[0018] 2. The scraper provided in the present invention facilitates the removal of adsorbed impurities and improves cleanliness. The driving motor drives the threaded screw to rotate, and the threaded screw rotates to move the threaded ring. The threaded ring moves through the connecting block to drive the strip plate to move. When the strip plate moves, it drives the guide block to slide on the guide rod. The guide rod and the guide block cooperate to make the strip plate move smoothly. When the strip plate moves, it drives the scraper to move. The movement of the scraper can scrape off the impurities adsorbed on the support plate, and the scraped impurities will enter the storage groove for storage, which is convenient for scraping and cleaning the impurities, maintaining cleanliness, and then the support plate can be cleaned to avoid impurity adsorption. After drying in the later stage, it is not easy to remove, thereby improving cleanliness.

[0019] 3. The present invention facilitates the transfer of cleaning liquid by providing a reaction tank. When cleaning and flushing the perfusion bottle, the solenoid valve on the waste liquid pipe is controlled so that the waste liquid pipe is no longer sealed, and the cleaning liquid flows to the outside through the waste liquid pipe, which facilitates the cleaning of the perfusion bottle. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1Schematic diagram of the structure of the perfusion device for stem cell culture of the present invention;

[0021] Figure 2 It is a schematic diagram of the framework structure of the present invention;

[0022] Figure 3 This is a schematic structural diagram of the perfusion bottle of the present invention;

[0023] Figure 4 It is a schematic structural diagram of the filter box of the present invention;

[0024] Figure 5 This is a schematic structural diagram of the square groove of the present invention;

[0025] Figure 6 Schematic diagram of the structure of the strip plate of the present invention;

[0026] Figure 7 It is a structural schematic diagram of the filter cartridge of the present invention;

[0027] Figure 8 It is a structural schematic diagram of the threaded sleeve and the threaded head of the present invention.

[0028] In the figure: 1. frame; 3. inspection port; 4. sealing door; 5. culture container; 6. connector No. 1; 7. connector No. 2; 8. perfusion bottle; 9. connecting pipe No. 1; 10. connecting pipe No. 2; 11. drainage pipe; 12. waste liquid pipe; 13. filter box; 14. liquid supply pipe; 15. through groove; 16. square groove; 17. sealing ring; 18. handle; 19. support plate; 20. threaded head; 21. threaded sleeve; 22. filter cartridge; 23. square frame; 24. connecting pipe; 25. pumping pump; 26. discharge pipe; 27. battery; 28. control button; 29. drive motor; 30. sliding groove; 31. threaded screw; 32. guide rod; 33. strip plate; 34. connecting block; 35. threaded ring; 36. receiving groove; 37. scraper; 38. guide block. DETAILED DESCRIPTION

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0030] See also Figure 1-8As shown, a perfusion device for stem cell culture comprises a frame 1, a closing component for easy maintenance is provided on the frame 1, a culture container 5 is provided inside the frame 1, a perfusion bottle 8 is fixedly installed inside the frame 1, a filter box 13 is fixedly installed inside the frame 1, a pumping component is provided inside the frame 1, a through groove 15 is provided on the top of the filter box 13, a connecting pipe 24 is connected to the filter box 13, a square groove 16 is passed through the through groove 15, a square frame 23 is fixedly connected to the square groove 16, a cleaning component is provided inside the square groove 16, a sealing ring 17 is connected to both the square groove 16 and the square frame 23, a support plate 19 is fixedly installed inside the square groove 16, a filter component for cell separation is provided on the support plate 19, when using the perfusion device for stem cell culture to culture stem cells, the culture container 5 is used to culture stem cells, the through groove 15 is provided to culture stem cells, and the through groove 15 is provided to culture stem cells. The stem cells cultured in the culture container 5 will flow into the perfusion bottle 8 through the No. 1 pipe 9, and the culture fluid will flow into the perfusion bottle 8 through the No. 1 connector 6. The perfusion fluid and nutrient solution can be added through the No. 1 connector 6, and cells can be cultured in the perfusion bottle 8. According to the set time, when the stem cells in the perfusion bottle 8 react, the solenoid valve on the liquid supply pipe 14 is controlled to open, so that the cells and culture fluid cultured in the perfusion bottle 8 flow into the filter box 13, and the cultured cells can be separated and collected by the filtering component, thereby achieving the purpose of facilitating the collection of the cultured cells. The separated liquid can be transmitted to the outside through the pumping component. After the cells are separated, the cleaning component moves, and the support plate 19 can be cleaned to avoid impurity adsorption. After drying up in the later stage, it is not easy to remove, thereby improving the cleanliness.

[0031] The closing component includes an inspection port 3 and a sealing door 4. The inspection port 3 is arranged on the side of the frame 1. The sealing door 4 is installed on the frame 1 through a hinge, and the sealing door 4 is used to seal the inspection port 3. The sealing door 4 can be moved to no longer seal the inspection port 3, and then the equipment inside the frame 1 can be easily inspected and maintained through the inspection port 3. A controller is provided on the frame 1 to control the operation of the electronic equipment.

[0032] A No. 1 connector 6 and a No. 2 connector 7 are installed on the frame 1, and the No. 1 connector 6 and the No. 2 connector 7 are symmetrically arranged. A No. 1 pipe 9 and a No. 2 pipe 10 are fixedly installed on the top of the perfusion bottle 8, and one end of the No. 2 pipe 10 is connected to the No. 1 connector 6, the No. 1 pipe 9 is connected to the bottom of the culture container 5, and the No. 2 connector 7 is connected to the culture container 5. The culture fluid can be added to the perfusion bottle 8 through the cooperation of the No. 1 connector 6 and the No. 2 pipe 10, and then the cells can be cultured. An electromagnetic valve is provided on the No. 1 pipe 9, which can control the opening and closing of the No. 1 pipe 9, so that the stem cells cultured in the culture container 5 can flow into the perfusion bottle 8 for cell cultivation, and the culture fluid flows.

[0033] Drain tubes 11 are symmetrically arranged at the bottom of the perfusion bottle 8, one of which is connected to a waste liquid tube 12, and one end of the waste liquid tube 12 extends out of the frame 1, and the other drain tube 11 is connected to a liquid supply tube 14. The liquid inside the perfusion bottle 8 flows to the outside through the drain tube 11. Both the waste liquid tube 12 and the liquid supply tube 14 are provided with solenoid valves. When the cells cultured inside the perfusion bottle 8 are collected, the solenoid valve on the liquid supply tube 14 is controlled to open, so that the liquid inside the perfusion bottle 8 can flow to the outside. When the perfusion bottle 8 is cleaned and rinsed, the solenoid valve on the waste liquid tube 12 is controlled so that the waste liquid tube 12 is no longer sealed, and the cleaning liquid flows to the outside through the waste liquid tube 12.

[0034] Solenoid valves for controlling opening and closing are provided on the No. 1 pipe 9, the No. 2 pipe 10, the waste liquid pipe 12 and the liquid supply pipe 14. A handle 18 is fixedly installed on the top of the square groove 16. A controller is provided on the side of the frame 1 to be electrically connected to the solenoid valve, thereby controlling the operation of the solenoid valve, and can control the opening and closing states of the No. 1 pipe 9, the No. 2 pipe 10, the waste liquid pipe 12 and the liquid supply pipe 14. Pulling the handle 18 can drive the square groove 16 to move, thereby driving the filtered cells to move to the outside world.

[0035] The filter assembly includes a threaded head 20, a threaded sleeve 21 and a filter cartridge 22. The threaded head 20 array is installed on the support plate 19. The threaded sleeve 21 is threadedly connected to the corresponding threaded head 20. The filter cartridge 22 is fixedly connected to the threaded sleeve 21. The filter cartridge 22 is covered with a filter membrane, which can allow the liquid to flow to the outside and intercept cells, so that the cells can be filtered and collected inside the filter cartridge 22. When processing the collected cells, the filter cartridge 22 is rotated to drive the threaded sleeve 21 to rotate, thereby separating the threaded sleeve 21 from the threaded head 20, and then the filter cartridge 22 can be disassembled to facilitate the separation and collection of cells.

[0036] The pumping assembly includes a pumping pump 25 and a discharge pipe 26. The pumping pump 25 is fixedly installed inside the frame 1, and the input end of the pumping pump 25 is connected to the connecting pipe 24. One end of the discharge pipe 26 is connected to the output end of the pumping pump 25, and one end of the discharge pipe 26 extends out of the frame 1. After the liquid filtered by the filter cartridge 22 flows into the filter box 13, it will flow into the pumping pump 25 through the connecting pipe 24. The pumping pump 25 can extract the liquid, and then the extracted liquid flows to the outside through the discharge pipe 26.

[0037] The cleaning assembly includes a driving mechanism, a guide mechanism, a strip plate 33, a receiving groove 36 and a scraper 37. The driving mechanism for driving the strip plate 33 to move is arranged on the square groove 16, and the guide mechanism for guiding the strip plate 33 is arranged inside the square groove 16. The receiving groove 36 is arranged on the strip plate 33, and the scraper 37 is fixedly installed on the strip plate 33, and the scraper 37 is in contact with the side of the support plate 19. The scraper 37 is arranged at an angle. When filtering and collecting cells, impurities carried by the culture medium are easily adsorbed on the support plate 19. When removing impurities, the movement of the driving mechanism will cause the strip plate 33 to move. When the strip plate 33 moves, it will drive the scraper 37 to move. The scraper 37 can move to scrape off the impurities adsorbed on the support plate 19. The scraped impurities will enter the receiving groove 36 for storage, thereby facilitating the scraping and cleaning of the impurities and maintaining cleanliness.

[0038] The driving mechanism includes a power supply battery 27, a control button 28, a drive motor 29, a threaded screw 31, a connecting block 34 and a threaded ring 35. Sliding grooves 30 are symmetrically arranged inside the square groove 16. The threaded screw 31 is arranged inside one of the sliding grooves 30. The connecting block 34 is fixedly connected to one end of the strip plate 33. The threaded ring 35 is fixedly arranged inside the connecting block 34, and the threaded screw 31 is threadedly connected to the threaded ring 35. The drive motor 29 is fixedly set at the top of the square groove 16, and one end of the threaded screw 31 is fixedly connected to the output end of the drive motor 29. The power supply battery 27 is fixedly installed on the top of the square frame 23, and the power supply battery 27 is electrically connected to the drive motor 29. A control button 28 is provided on the power supply battery 27, and the control button 28 is electrically connected to the drive motor 29. Controlling the drive motor 29 by the control button 28 will drive the threaded screw 31 to rotate. When the threaded screw 31 rotates, the threaded ring 35 will move. The threaded ring 35 moves through the connecting block 34 to drive the strip plate 33 to move.

[0039] The guide mechanism includes a guide rod 32 and a guide block 38. The guide block 38 is fixedly mounted on the strip plate 33. The guide rod 32 is fixedly mounted inside another sliding groove 30. The guide block 38 is fixedly mounted at one end of the strip plate 33, and the guide rod 32 passes through the guide block 38. When the strip plate 33 moves, it drives the guide block 38 to slide on the guide rod 32. The cooperation between the guide rod 32 and the guide block 38 can make the strip plate 33 move smoothly.

[0040] As a technical optimization solution of the present invention, when a perfusion device for stem cell culture is used to culture stem cells, the culture container 5 is used to culture stem cells. The stem cells cultured in the culture container 5 will flow into the perfusion bottle 8 through the No. 1 pipe 9, and the culture fluid will flow into the perfusion bottle 8 through the No. 1 connector 6. The perfusion fluid and nutrient solution can be added through the No. 1 connector 6 to culture cells in the perfusion bottle 8. After the stem cells in the perfusion bottle 8 react after the set time, the solenoid valve on the liquid supply pipe 14 is controlled to open, so that the cells and culture fluid cultured in the perfusion bottle 8 flow into the filter box 13. The filter cartridge 22 is covered with a filter membrane, which can make the liquid flow to the outside and intercept the cells, so that the cells can be filtered and collected in the filter cartridge 22. After the liquid filtered by the filter cartridge 22 flows into the filter box 13, it will flow into the pumping pump 25 through the connecting pipe 24. The pumping pump 25 can extract the liquid, and the extracted liquid flows to the outside through the discharge pipe 26. When the collected cells are processed, pull the handle The hand 18 can drive the square groove 16 to move, thereby driving the filtered cells to move to the outside world, and then rotating the filter cartridge 22 to drive the threaded sleeve 21 to rotate, thereby separating the threaded sleeve 21 from the threaded head 20, and then the filter cartridge 22 can be disassembled to facilitate the separation and collection of cells. After the cells are separated, the control button 28 is used to control the drive motor 29 to drive the threaded screw 31 to rotate, and the threaded screw 31 rotates to move the threaded ring 35. The threaded ring 35 moves through the connecting block 34 to drive the strip plate 33 to move, and the strip plate 33 is moved. When the plate 33 moves, it will drive the guide block 38 to slide on the guide rod 32. The cooperation between the guide rod 32 and the guide block 38 will make the strip plate 33 move smoothly. When the strip plate 33 moves, it will drive the scraper 37 to move. The movement of the scraper 37 can scrape off the impurities adsorbed on the support plate 19. The scraped impurities will enter the storage groove 36 for storage, which makes it easier to scrape and clean the impurities and maintain cleanliness. The support plate 19 can then be cleaned to avoid impurity adsorption. After drying up, it is not easy to remove, which improves cleanliness.

[0041] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0042] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A perfusion device for stem cell culture, characterized in that: The invention comprises a frame (1), wherein the frame (1) is provided with a closing component for easy maintenance, a culture container (5) is provided inside the frame (1), a perfusion bottle (8) is fixedly installed inside the frame (1), a filter box (13) is fixedly installed inside the frame (1), a pumping component is provided inside the frame (1), a through groove (15) is provided on the top of the filter box (13), a connecting pipe (24) is connected to the filter box (13), a square groove (16) is provided inside the through groove (15), a square frame (23) is fixedly connected to the square groove (16), a cleaning component is provided inside the square groove (16), a sealing ring (17) is connected to both the square groove (16) and the square frame (23), a support plate (19) is fixedly installed inside the square groove (16), and a filter component for cell separation is provided on the support plate (19).

2. A perfusion device for stem cell culture according to claim 1, characterized in that: The closing assembly comprises an inspection port (3) and a sealing door (4); the inspection port (3) is arranged on the side of the frame (1); the sealing door (4) is mounted on the frame (1) via a hinge, and the sealing door (4) is used to seal the inspection port (3).

3. The perfusion device for stem cell culture according to claim 1, wherein: A No. 1 connector (6) and a No. 2 connector (7) are installed on the frame (1), and the No. 1 connector (6) and the No. 2 connector (7) are symmetrically arranged. A No. 1 pipe (9) and a No. 2 pipe (10) are fixedly installed on the top of the perfusion bottle (8), and one end of the No. 2 pipe (10) is connected to the No. 1 connector (6), the No. 1 pipe (9) is connected to the bottom of the culture container (5), and the No. 2 connector (7) is connected to the culture container (5).

4. A perfusion device for stem cell culture according to claim 3, characterized in that: Drain pipes (11) are symmetrically arranged at the bottom of the perfusion bottle (8), one of the drainage pipes (11) is connected to a waste liquid pipe (12), and one end of the waste liquid pipe (12) extends out of the frame (1), and the other drainage pipe (11) is connected to a liquid supply pipe (14).

5. A perfusion device for stem cell culture according to claim 4, characterized in that: The No. 1 pipe (9), the No. 2 pipe (10), the waste liquid pipe (12) and the liquid supply pipe (14) are all provided with electromagnetic valves for controlling opening and closing, and a handle (18) is fixedly installed on the top of the square groove (16).

6. The perfusion device for stem cell culture according to claim 1, wherein: The filter assembly comprises a threaded head (20), a threaded sleeve (21) and a filter cartridge (22); the threaded head (20) is arrayed on a support plate (19); the threaded sleeve (21) is threadedly connected to the corresponding threaded head (20); and the filter cartridge (22) is fixedly connected to the threaded sleeve (21).

7. The perfusion device for stem cell culture according to claim 1, wherein: The pumping assembly comprises a pumping pump (25) and a discharge pipe (26), wherein the pumping pump (25) is fixedly installed inside the frame (1), and the input end of the pumping pump (25) is connected to the connecting pipe (24), and one end of the discharge pipe (26) is connected to the output end of the pumping pump (25), and one end of the discharge pipe (26) extends out of the frame (1).

8. The perfusion device for stem cell culture according to claim 1, wherein: The cleaning assembly comprises a driving mechanism, a guiding mechanism, a strip plate (33), a receiving groove (36) and a scraper (37), wherein the driving mechanism for driving the strip plate (33) to move is arranged on the square groove (16), the guiding mechanism for guiding the strip plate (33) is arranged inside the square groove (16), the receiving groove (36) is arranged on the strip plate (33), the scraper (37) is fixedly mounted on the strip plate (33), and the scraper (37) is in contact with the side of the support plate (19), and the scraper (37) is arranged at an angle.

9. The perfusion device for stem cell culture according to claim 8, characterized in that: The driving mechanism comprises a power supply battery (27), a control button (28), a driving motor (29), a screw thread rod (31), a connecting block (34) and a screw thread ring (35). The sliding grooves (30) are symmetrically arranged inside the square groove (16). The screw thread rod (31) is arranged inside one of the sliding grooves (30). The connecting block (34) is fixedly connected to one end of the strip plate (33). The screw thread ring (35) is fixedly arranged inside the connecting block (34). The screw thread rod (31) is threadedly connected to the screw thread ring (35). The driving motor (29) is fixedly arranged at the top of the square groove (16). One end of the screw thread rod (31) is fixedly connected to the output end of the driving motor (29). The power supply battery (27) is fixedly installed on the top of the square frame (23). The power supply battery (27) is electrically connected to the driving motor (29). A control button (28) is arranged on the power supply battery (27). The control button (28) is electrically connected to the driving motor (29).

10. The perfusion device for stem cell culture according to claim 9, characterized in that: The guide mechanism comprises a guide rod (32) and a guide block (38), wherein the guide block (38) is fixedly mounted on the strip plate (33), the guide rod (32) is fixedly mounted inside another sliding groove (30), the guide block (38) is fixedly mounted on one end of the strip plate (33), and the guide rod (32) passes through the guide block (38).

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