Cell negative pressure culture device

Abstract

The invention belongs to the technical field of medical instruments, and particularly relates to a cell negative pressure culture device which comprises a sealing box body, a negative pressure maintaining mechanism, a culture solution injection mechanism, a cell storing and taking mechanism and a monitoring mechanism, the negative pressure maintaining mechanism is arranged on the side wall of the sealing box body, the culture solution injection mechanism is arranged in the sealing box body, and the cell storing and taking mechanism is arranged in the sealing box body. The cell access mechanism is arranged on the inner wall of the sealed box body, and the monitoring mechanism is arranged at the top end of the inner wall of the sealed box body. Through the innovatively designed sealing mechanism, automatic opening and closing of the sealing door are achieved, internal and external substance exchange is completed in a sealing state, and external pollution is effectively isolated; the cell storing and taking mechanism can realize automatic storing and taking of cell samples in a sealed environment, and the problem that the sealed environment is damaged by sample replacement is solved; the pressure stabilizing mechanism calculates the pressure difference according to the required negative pressure, places a pressure stabilizing weight to offset the pressure, cooperates with a pressure stabilizing piston and a switch to control a negative pressure pump, automatically maintains the pressure in the box stable, and reduces the air pressure fluctuation.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and specifically relates to a cell negative pressure culture device. Background Art

[0002] Cell culture technology is an important means of studying cell behavior, physiological functions, and disease mechanisms. Traditional cell culture is typically conducted under normal pressure or slightly positive pressure. Negative pressure can simulate certain physiological or pathological conditions in the body (such as the microenvironmental pressure changes during wound repair and tissue regeneration), significantly affecting cell proliferation, migration, differentiation, and metabolic activities.

[0003] Currently, conventional cell culture devices (such as culture dishes, culture flasks, or multi-well plates) are unable to actively regulate the pressure in the culture environment, especially unable to achieve stable negative pressure conditions. Some studies have briefly applied negative pressure through customized negative pressure systems (such as vacuum pumps or syringes), but there are problems such as large pressure fluctuations, difficulty in precise control, and inability to maintain long-term maintenance, resulting in poor reproducibility of experimental results. In order to observe the growth of cells in a negative pressure environment, cells are usually cultured on cell culture multi-well plates and cell culture vessels, and a fixed amount of culture fluid is injected once before the negative pressure seal is established. If the culture fluid needs to be injected again, the negative pressure seal of the cell culture device must be released. In the process of injecting the culture fluid, the sterile sealing environment and negative pressure state of the cell culture device are destroyed, which can easily contaminate the cells and affect the accuracy of observation.

[0004] There is a need for a culture device that can provide a stable culture environment. Therefore, a cell stable negative pressure culture device is proposed. Summary of the Invention

[0005] In order to solve the above-mentioned existing problems, the present invention provides a cell negative pressure culture device. The present invention uses its innovatively designed sealing mechanism, through the forward and reverse rotation of the sealing servo motor, in conjunction with gear transmission and spring elasticity, to realize automatic opening and closing of the sealed door, complete the internal and external material exchange in a sealed state, and effectively isolate external pollution; the cell storage and retrieval mechanism uses servo motors, hydraulic devices and other components to realize automatic storage and retrieval of cell samples in a sealed environment, solving the problem of sample replacement destroying the sealed environment; the pressure stabilizing mechanism calculates the pressure difference according to the required negative pressure, places a pressure stabilizing weight to offset the pressure, and cooperates with the pressure stabilizing piston and switch to control the negative pressure pump to automatically maintain the pressure in the box stable and reduce air pressure fluctuations; the culture fluid injection mechanism can accurately inject culture fluid by adjusting the position of the grabbing frame in a sealed negative pressure environment, avoiding the risk of pollution caused by pressure release and decompression; the cell sampling mechanism can control the grabbing frame to insert the glass dropper into the culture dish, so as to achieve sampling and observation without interfering with other cell cultures, making up for the deficiency that the existing technology cannot take samples.

[0006] The technical solution adopted by the present invention is as follows: This solution provides a cell negative pressure culture device, including a sealed box, a negative pressure maintaining mechanism, a culture fluid injection mechanism, a cell access mechanism and a monitoring mechanism, wherein the negative pressure maintaining mechanism is arranged on the side wall of the sealed box, the culture fluid injection mechanism is arranged in the sealed box, the cell access mechanism is arranged on the inner wall of the sealed box, and the monitoring mechanism is arranged on the top of the inner wall of the sealed box. The negative pressure maintaining mechanism includes a pressure stabilizing mechanism and a sealing mechanism, the pressure stabilizing mechanism is arranged on the side wall of the sealed box, the sealing mechanism is arranged on the side wall of the sealed box, the cell access mechanism includes a cell dish access mechanism and a cell sampling mechanism, the cell dish access mechanism is arranged on the inner wall of the sealed box, the cell sampling mechanism is arranged on the cell dish access mechanism, and a storage rack is fixedly provided on the bottom wall inside the sealed box.

[0007] Furthermore, the pressure-stabilizing mechanism includes a negative pressure pump, a pressure-stabilizing groove, a pressure-stabilizing piston, a pressure-stabilizing rope, a pressure-stabilizing sealing ring, a pressure-stabilizing wheel, a pressure-stabilizing frame, a pressure-stabilizing switch and a pressure-stabilizing weight. The negative pressure pump is fixed on the outer wall of the sealed box body, the input end of the negative pressure pump passes through the side wall of the sealed box body, the pressure-stabilizing groove is opened through the side wall of the sealed box body, the pressure-stabilizing piston is slidingly arranged on the inner wall of the pressure-stabilizing groove, the pressure-stabilizing sealing ring is arranged in pairs on the circumferential outer wall of the pressure-stabilizing piston, the pressure-stabilizing wheel is rotatably arranged on the outer wall of the sealed box body, one end of the pressure-stabilizing rope is fixed on the side wall of the pressure-stabilizing piston, the top of the pressure-stabilizing frame is fixed on the other end of the pressure-stabilizing rope, the pressure-stabilizing switches are fixed in pairs on the inner wall of the pressure-stabilizing groove, and the pressure-stabilizing weights are arranged on the pressure-stabilizing frame.

[0008] Furthermore, the sealing mechanism includes a transfer chamber, an access door, a sealing door, an access rod, a sealing rod, a joint control arc block, a joint control groove 1, a joint control groove 2, a sealing spring 1, a sealing spring 2, a sealing servo motor, a sealing gear, a sealing gear and a sealing gasket. The transfer chamber is opened on the inner wall of the sealing box, the sealing door is rotatably arranged on the side wall of one end of the transfer chamber located in the sealing box, the access door is rotatably arranged on the side wall of one end of the transfer chamber located in the sealing box, the sealing door is communicated with the outside of the sealing box, the sealing rod is fixedly arranged on the side wall of the sealing door, the access rod is fixedly arranged on the side wall of the access door, the joint control arc block is slidably arranged on the inner wall of the transfer chamber, the joint control groove 1 is opened It is arranged on the joint control arc block, the joint control groove 2 is opened on the joint control arc block, the sealing rod is slidingly arranged on the inner wall of the joint control groove 1, the access rod is slidingly arranged on the inner wall of the joint control groove 2, one end of the sealing spring 1 is fixedly arranged on one side wall of the joint control groove, the other end of the sealing spring 1 is fixedly connected to the side wall of the sealing rod, one end of the sealing spring 2 is fixedly arranged on the side wall of the joint control groove 2, the other end of the sealing spring 2 is fixedly connected to the side wall of the access rod, the sealing servo motor is fixedly arranged on the inner wall of the transfer chamber, the sealing gear is coaxially fixedly arranged on the output end of the sealing servo motor, the sealing gear array is fixedly arranged on the side wall of the joint control arc block, and the sealing gasket is fixedly arranged on the side walls of the access door and the sealing door.

[0009] Furthermore, the cell dish access mechanism includes an access slot, an access servo motor, an access threaded rod, an access rack, a vertical hydraulic pressure, a grabbing rack, a telescopic hydraulic pressure, a telescopic rack, a grabbing claw and a grabbing hydraulic pressure. The access slot is opened on the inner wall of the sealed box, the access servo motor is fixed on the side wall of the access slot, the access threaded rod is coaxially fixed on the output end of the access servo motor, the access rack is slidably arranged on the inner wall of the access slot, one end of the access threaded rod passes through the side wall of the access rack, one end of the vertical hydraulic pressure is fixed on the side wall of the access rack, the grabbing rack is fixed on the output end of the vertical hydraulic pressure, the telescopic hydraulic pressure is fixed on the side wall of the grabbing rack, the telescopic rack is fixed on the output end of the telescopic hydraulic pressure, the grabbing claws are rotatably arranged on the side wall of the telescopic rack in pairs, one end of the grabbing hydraulic pressure is rotatably arranged on the side wall of the telescopic rack, and the grabbing hydraulic pressure is rotatably connected to the side wall of the grabbing claw.

[0010] Furthermore, the cell sampling mechanism includes a sampling trough, a glass dropper and a pressing hydraulic device, the sampling trough is opened on the telescopic frame, the glass dropper is arranged in the sampling trough, and the pressing hydraulic device is fixed to the inner wall of the sampling trough.

[0011] Furthermore, the culture fluid injection mechanism includes an injection needle and an injection tube. The injection needle is fixed to the side wall of the telescopic frame. One end of the injection tube is fixed to the top of the injection needle. The injection needle is connected to the injection tube. The other end of the injection tube passes through the side wall of the sealed box.

[0012] Furthermore, the monitoring mechanism includes a pressure sensor, a temperature sensor, a real-time camera and a real-time screen. The pressure sensor is fixed on the inner wall of the sealed box, the temperature sensor is fixed on the inner wall of the sealed box, the real-time camera is fixed on the inner wall of the sealed box, and the real-time screen is fixed on the outer wall of the sealed box.

[0013] Furthermore, an electrical control panel is fixedly provided on the outer wall of the sealed box, and the negative pressure pump, sealing servo motor, access servo motor, vertical hydraulic press, telescopic hydraulic press, grabbing hydraulic press, pressing hydraulic press, pressure sensor, temperature sensor, real-time camera and real-time screen are electrically connected to the electrical control panel through wires.

[0014] Furthermore, the access threaded rod is connected to the access rack through a threaded connection, the sealing gear is engaged with the sealing gear teeth, the linkage control groove 1 and the linkage control groove 2 are arc-shaped, the sealing door coincides with the access door rotation axis, the arc centers of the linkage control groove 1 and the linkage groove 2 coincide with the sealing door and the access door rotation axis, and the sealing rod and the access rod are arc-shaped.

[0015] Furthermore, the inner side of the grabbing claw is arc-shaped, and the large end of the glass dropper is made of rubber.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The present invention innovatively designs a sealing mechanism. The sealing servo motor rotates forward, and the sealing gear and the sealing gear teeth drive the joint control arc block to rotate clockwise, so that the sealing rod rotates with the joint control slot 1, and then the sealing door is opened. When the joint control arc block rotates, the joint control slot 2 rotates accordingly. The sealing spring 2 is squeezed between the access rod and the side wall of the joint control slot 2. As the access door is closed, the access rod is limited clockwise, and the spring 2 is further compressed. Its elastic force makes the access door close to the inner wall of the sealed box. The user can put the culture dish containing cells into the transfer chamber to realize automatic door opening in a sealed state, effectively isolating the external environment and reducing the risk of contamination. When the sealing servo motor is reversed, the transmission The locking mechanism drives the joint control arc block to rotate counterclockwise. Under the elastic action of the sealing spring 1, the sealing rod rotates counterclockwise, the sealed door is closed, the access rod slides in the joint control groove 2, the spring 2 is compressed and restored, and the access rod contacts the side wall of one end of the joint control groove 2 and is driven to rotate counterclockwise, and the access door is opened. At the same time, the joint control arc block drives the joint control groove 1 to rotate. The sealing spring 1 is squeezed between the sealing rod and the side wall of the joint control groove. The access rod is limited counterclockwise due to the closing of the sealed door. The spring 1 is further compressed, and its elastic force makes the access door close to the inner wall of the sealed box, so that the culture dish automatically enters the culture environment, reducing external infection and human error, and completing the internal and external material exchange in a sealed state.

[0018] (2) The present invention is provided with a cell access mechanism, and the access servo motor is operated, and the access rack is driven by the access threaded rod to slide on the inner wall of the access slot. At the same time, the vertical hydraulic actuator and the telescopic hydraulic actuator are controlled by the electrical control panel to adjust the height and horizontal position of the grabbing rack and the grabbing claw, so that the grabbing claw moves and penetrates into the transfer chamber. The user controls the grabbing hydraulic actuator through the electrical control panel to rotate the two grabbing claws on the side wall of the grabbing rack and approach each other to grab the cell culture dish. Subsequently, by controlling the access servo motor, the vertical hydraulic actuator and the telescopic hydraulic actuator, the cell culture dish is moved three-dimensionally in the sealed box and placed on the access rack. By repeating the above operation, multiple cell culture dishes can be placed on the access rack in turn, achieving the technical effect of automatically accessing cell samples in a sealed environment, and effectively solving the problem that the replacement of cell samples in the prior art will destroy the negative pressure sealing environment;

[0019] (3) The present invention is provided with a pressure stabilizing mechanism. According to the negative pressure required for cell culture, the pressure difference between the internal and external pressure differences of the sealed box on the pressure stabilizing piston is calculated, and a corresponding number of pressure stabilizing weights are placed on the pressure stabilizing frame accordingly. The gravity of the pressure stabilizing weights is used to offset the pressure difference on the pressure stabilizing piston. When the internal pressure is greater than the preset pressure, the pressure difference at both ends of the pressure stabilizing piston decreases, and the pressure stabilizing piston moves toward the outside of the sealed box in the pressure stabilizing groove until it touches the pressure stabilizing switch, triggering the negative pressure pump to start, and exhausting the inside of the sealed box to reduce the internal pressure. When the internal pressure is less than the preset pressure, the pressure difference at both ends of the pressure stabilizing piston increases, and the pressure stabilizing piston moves toward the inside of the sealed box in the pressure stabilizing groove until it touches the pressure stabilizing switch on the other side of the pressure stabilizing groove, triggering the negative pressure pump to shut down, thereby achieving the technical effect of automatically maintaining the internal pressure of the sealed box. At the same time, the internal pressure of the sealed box can be adjusted by replacing the number of pressure stabilizing weights, and the pressure stabilizing piston is always in a dynamic equilibrium state, reducing pressure fluctuations.

[0020] (4) The present invention is provided with a culture medium injection mechanism. When the cell culture dish inside the sealed box lacks culture medium, the user can adjust the position of the grabbing frame to insert the injection needle into the cell culture dish, and inject the culture medium into the cell culture dish from the outside through the injection tube and the injection needle through the external culture medium injection device, thereby achieving the technical effect of completing the filling of the cell culture dish while maintaining a sealed and negative pressure environment, effectively solving the problem of the need to release and reduce pressure again in the prior art, and avoiding cell contamination caused by the addition of nutrient solution;

[0021] (5) The present invention is provided with a cell sampling mechanism. When it is necessary to sample and observe the cells in the cell culture dish in detail, the user can control the position of the grabbing frame, insert the glass dropper into the cell culture dish to be sampled and observed, and control the hydraulic press to extend and retract through the electrical control panel to squeeze the large end of the glass dropper in the sampling groove to suck out the cells in the cell culture dish. Then, according to the above steps, the cells are transferred to the outside through the transfer chamber, thereby achieving the technical effect of sampling and observing cells without affecting other cell cultures, and effectively solving the problem of being unable to sample and observe in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a front view of a negative pressure cell culture device proposed by the present invention;

[0023] Figure 2 This is a three-dimensional diagram of a negative pressure cell culture device proposed by the present invention;

[0024] Figure 3 This is a three-dimensional internal structure diagram of a cell negative pressure culture device proposed by the present invention;

[0025] Figure 4 This is a right view of a negative pressure cell culture device proposed by the present invention;

[0026] Figure 5 This is a front cross-sectional view of a negative pressure cell culture device proposed by the present invention;

[0027] Figure 6 for Figure 5 Enlarged view of part A in the middle;

[0028] Figure 7 This is a top cross-sectional view of a negative pressure cell culture device proposed by the present invention;

[0029] Figure 8 for Figure 7 Enlarged view of middle part B;

[0030] Figure 9 This is a first stereoscopic cross-sectional view of a negative pressure cell culture device proposed by the present invention;

[0031] Figure 10 for Figure 9 Enlarged view of middle C part;

[0032] Figure 11 This is a second stereoscopic cross-sectional view of a negative pressure cell culture device proposed by the present invention;

[0033] Figure 12 for Figure 11 Enlarged view of part D in the middle.

[0034] Among them, 1. Sealed box, 2. Negative pressure maintaining mechanism, 3. Culture fluid injection mechanism, 4. Cell access mechanism, 5. Monitoring mechanism, 210. Pressure stabilizing mechanism, 220. Sealing mechanism, 410. Cell dish access mechanism, 420. Cell sampling mechanism, 101. Storage rack, 211. Negative pressure pump, 212. Pressure stabilizing tank, 213. Pressure stabilizing piston, 214. Pressure stabilizing rope, 215. Pressure stabilizing sealing ring, 216. Pressure stabilizing wheel, 217. Pressure stabilizing rack, 218. Pressure stabilizing switch, 219. Pressure stabilizing weight, 221. Transfer chamber, 222. Access door, 223. Sealing door, 224. Access rod, 225. Sealing rod, 226. Joint control arc block, 227. Joint control tank one, 228. Joint control tank two, 229. Sealing spring 1, 2310, sealing spring 2, 2311, sealing servo motor, 2312, sealing gear, 2313, sealing gear teeth, 2314, sealing gasket, 411, access slot, 412, access servo motor, 413, access threaded rod, 414, access rack, 415, vertical hydraulic press, 416, grabbing rack, 417, telescopic hydraulic press, 418, telescopic rack, 419, grabbing claw, 4110, grabbing hydraulic press, 421, sampling slot, 422, glass dropper, 423, pressing hydraulic press, 301, injection needle, 302, injection tube, 501, pressure sensor, 502, temperature sensor, 503, real-time camera, 504, real-time screen, 102, electrical control panel.

[0035] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0036] The present invention will be further described in detail with reference to the accompanying drawings.

[0037] like Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 、 Figure 9 、 Figure 10 、 Figure 11 and Figure 12 As shown, the present solution provides a cell negative pressure culture device, comprising a sealed box 1, a negative pressure maintaining mechanism 2, a culture fluid injection mechanism 3, a cell access mechanism 4 and a monitoring mechanism 5. The negative pressure maintaining mechanism 2 is arranged on the side wall of the sealed box 1, the culture fluid injection mechanism 3 is arranged in the sealed box 1, the cell access mechanism 4 is arranged on the inner wall of the sealed box 1, and the monitoring mechanism 5 is arranged at the top of the inner wall of the sealed box 1. The negative pressure maintaining mechanism 2 includes a pressure stabilizing mechanism 210 and a sealing mechanism 220. The pressure stabilizing mechanism 210 is arranged on the side wall of the sealed box 1, and the sealing mechanism 220 is arranged on the side wall of the sealed box 1. The cell access mechanism 4 includes a cell dish access mechanism 410 and a cell sampling mechanism 420. The cell dish access mechanism 410 is arranged on the inner wall of the sealed box 1, and the cell sampling mechanism 420 is arranged on the cell dish access mechanism 410. A storage rack 101 is fixedly provided on the bottom wall of the sealed box 1.

[0038] The pressure stabilizing mechanism 210 includes a negative pressure pump 211, a pressure stabilizing groove 212, a pressure stabilizing piston 213, a pressure stabilizing rope 214, a pressure stabilizing seal 215, a pressure stabilizing wheel 216, a pressure stabilizing frame 217, a pressure stabilizing switch 218 and a pressure stabilizing weight 219. The negative pressure pump 211 is fixed to the outer wall of the sealed box 1, the input end of the negative pressure pump 211 passes through the side wall of the sealed box 1, the pressure stabilizing groove 212 passes through the side wall of the sealed box 1, and the pressure stabilizing piston 213 is provided. 13 is slidingly arranged on the inner wall of the pressure-stabilizing groove 212, the pressure-stabilizing sealing rings 215 are sleeved in pairs on the circumferential outer wall of the pressure-stabilizing piston 213, the pressure-stabilizing wheel 216 is rotatably arranged on the outer wall of the sealing box body 1, one end of the pressure-stabilizing rope 214 is fixedly arranged on the side wall of the pressure-stabilizing piston 213, the top of the pressure-stabilizing frame 217 is fixedly arranged on the other end of the pressure-stabilizing rope 214, the pressure-stabilizing switches 218 are fixed in pairs on the inner wall of the pressure-stabilizing groove 212, and the pressure-stabilizing weights 219 are arranged on the pressure-stabilizing frame 217.

[0039] Among them, the sealing mechanism 220 includes a transfer chamber 221, an access door 222, a sealing door 223, an access rod 224, a sealing rod 225, a joint control arc block 226, a joint control slot 1 227, a joint control slot 2 228, a sealing spring 1 229, a sealing spring 2 2310, a sealing servo motor 2311, a sealing gear 2312, a sealing gear 2313 and a sealing gasket 2314. The transfer chamber 221 is opened on the inner wall of the sealing box body 1, the sealing door 223 is rotatably arranged on one end side wall of the transfer chamber 221 located in the sealing box body 1, the access door 222 is rotatably arranged on one end side wall of the transfer chamber 221 located in the sealing box body 1, the sealing door 223 is communicated with the outside of the sealing box body 1, the sealing rod 225 is fixedly arranged on the side wall of the sealing door 223, the access rod 224 is fixedly arranged on the side wall of the access door 222, and the joint control arc block 226 is slidably arranged on the inner wall of the transfer chamber 221. The linkage groove 1 227 is opened on the linkage arc block 226, the linkage groove 228 is opened on the linkage arc block 226, the sealing rod 225 is slidably arranged on the inner wall of the linkage groove 1 227, the access rod 224 is slidably arranged on the inner wall of the linkage groove 228, one end of the sealing spring 1 229 is fixedly arranged on the side wall of the linkage groove 1 227, and the other end of the sealing spring 1 229 is fixedly connected to the side wall of the sealing rod 225, one end of the sealing spring 2 2310 is fixedly arranged on the side wall of the linkage groove 228, and the other end of the sealing spring 2 2310 is fixedly connected to the side wall of the access rod 224, the sealing servo motor 2311 is fixedly arranged on the inner wall of the transfer chamber 221, the sealing gear 2312 is coaxially fixedly arranged on the output end of the sealing servo motor 2311, the sealing gear 2313 array is fixedly arranged on the side wall of the linkage arc block 226, and the sealing gasket 2314 is fixedly arranged on the side walls of the access door 222 and the sealing door 223.

[0040] Among them, the cell dish access mechanism 410 includes an access slot 411, an access servo motor 412, an access threaded rod 413, an access rack 414, a vertical hydraulic press 415, a grabbing rack 416, a telescopic hydraulic press 417, a telescopic rack 418, a grabbing claw 419 and a grabbing hydraulic press 4110. The access slot 411 is opened on the inner wall of the sealed box 1, the access servo motor 412 is fixedly arranged on the side wall of the access slot 411, the access threaded rod 413 is coaxially fixed to the output end of the access servo motor 412, and the access rack 414 is slidably arranged in the access slot 411. Wall, one end of the access threaded rod 413 passes through the side wall of the access frame 414, one end of the vertical hydraulic press 415 is fixed on the side wall of the access frame 414, the grabbing frame 416 is fixed on the output end of the vertical hydraulic press 415, the telescopic hydraulic press 417 is fixed on the side wall of the grabbing frame 416, the telescopic frame 418 is fixed on the output end of the telescopic hydraulic press 417, the grabbing claws 419 are rotatably arranged in pairs on the side wall of the telescopic frame 418, one end of the grabbing hydraulic press 4110 is rotatably arranged on the side wall of the telescopic frame 418, and the grabbing hydraulic press 4110 is rotatably connected to the side wall of the grabbing claw 419.

[0041] The cell sampling mechanism 420 includes a sampling slot 421 , a glass dropper 422 and a pressing hydraulic device 423 . The sampling slot 421 is provided on the telescopic frame 418 , the glass dropper 422 is provided in the sampling slot 421 , and the pressing hydraulic device 423 is fixed to the inner wall of the sampling slot 421 .

[0042] Among them, the culture fluid injection mechanism 3 includes an injection needle 301 and an injection tube 302. The injection needle 301 is fixed on the side wall of the telescopic frame 418. One end of the injection tube 302 is fixed on the top of the injection needle 301. The injection needle 301 is connected to the injection tube 302. The other end of the injection tube 302 passes through the side wall of the sealed box 1.

[0043] Among them, the monitoring mechanism 5 includes a pressure sensor 501, a temperature sensor 502, a real-time camera 503 and a real-time screen 504. The pressure sensor 501 is fixed on the inner wall of the sealed box 1, the temperature sensor 502 is fixed on the inner wall of the sealed box 1, the real-time camera 503 is fixed on the inner wall of the sealed box 1, and the real-time screen 504 is fixed on the outer wall of the sealed box 1.

[0044] Among them, an electrical control panel 102 is fixedly installed on the outer wall of the sealed box 1, and the negative pressure pump 211, the sealing servo motor 2311, the access servo motor 412, the vertical hydraulic press 415, the telescopic hydraulic press 417, the grabbing hydraulic press 4110, the pressing hydraulic press 423, the pressure sensor 501, the temperature sensor 502, the real-time camera 503 and the real-time screen 504 are electrically connected to the electrical control panel 102 through wires.

[0045] Among them, the access threaded rod 413 is connected to the access frame 414 through a threaded connection, the sealing gear 2312 is meshed with the sealing gear teeth 2313, the linkage control groove 1 227 and the linkage control groove 2 28 are arc-shaped, the sealing door 223 coincides with the rotating axis of the access door 222, the center of the arc of the linkage control groove 1 227 and the linkage groove 2 coincides with the rotating axis of the sealing door 223 and the access door 222, and the sealing rod 225 and the access rod 224 are arc-shaped.

[0046] Among them, the inner side of the grabbing claw 419 is arc-shaped, and the large end of the glass dropper 422 is made of rubber material.

[0047] When in use, first connect one end of the injection tube 302 to the external nutrient solution injection system, and then the user can control the sealing servo motor 2311 to rotate through the electrical control panel 102, which is transmitted through the sealing gear 2312 and the sealing gear 2313, thereby driving the joint control arc block 226 to rotate clockwise, and then the sealing rod 225 is driven by the joint control groove 1 227 to rotate clockwise, and then the sealing door 223 can be rotated, that is, the sealing door 223 is opened. When the joint control arc block 226 rotates, it also drives the joint control groove 228 to rotate, thereby causing the sealing spring 2310 to move between the access rod 224 and the joint control groove 227. 8 side wall is squeezed. Since the access door 222 is already in the closed state, the access rod 224 is limited in the clockwise direction, which can further compress the sealing spring 2310. Due to the compression elastic force of the sealing spring 2310, the access rod 224 is always under clockwise pressure, thereby driving the access door 222 to stick tightly to the inner wall of the sealed box 1. Then, the user can place the culture dish containing cells in the transfer chamber 221, thereby achieving the technical effect of automatically opening the sealing door 223 in the sealed state, effectively isolating the influence of the external environment on the cell culture and reducing the risk of cell contamination.

[0048] Then the user can control the sealing servo motor 2311 to reverse through the electrical control panel 102, which is transmitted through the sealing gear 2312 and the sealing gear teeth 2313, thereby driving the joint control arc block 226 to rotate counterclockwise. Due to the elasticity of the sealing spring 1 229, the sealing rod 225 will be driven to rotate counterclockwise, that is, the sealing door 223 is closed. At this time, the access rod 224 is also sliding in the contact joint control groove 228, and the compression of the sealing spring 2310 will slowly return to its initial state until the access rod 224 contacts the side wall of one end of the joint control groove 228. As the joint control arc block 226 rotates further, the access rod 224 can be driven by the joint control groove 228 to rotate counterclockwise, thereby rotating the access door 222, that is, the access rod 224 is also sliding in the contact joint control groove 228. When the door 222 is opened and the joint control arc block 226 rotates, the joint control groove 1 227 is driven to rotate at the same time, thereby causing the sealing spring 1 229 to be squeezed between the sealing rod 225 and the side wall of the joint control groove 1 227. Since the sealing door 223 is already in the closed state, the access rod 224 is limited in the counterclockwise direction, thereby further compressing the sealing spring 1 229. Due to the compression elastic force of the sealing spring 1 229, the access rod 224 is always under counterclockwise pressure, thereby driving the sealing door 223 to stick tightly to the inner wall of the sealed box body 1, thereby achieving the technical effect of automatically entering the culture dish into the culture environment, reducing external infection and human error, and completing the exchange of internal and external substances while ensuring the sealing state;

[0049] Then the user can control the access servo motor 412 through the electrical control panel 102 to work, and the access threaded rod 413 is driven to make the access rack 414 slide on the inner wall of the access slot 411. At the same time, the vertical hydraulic machine 415 and the telescopic hydraulic machine 417 can be controlled by the electrical control panel 102 to work, thereby adjusting the height and horizontal position of the grabbing rack 416 and the grabbing claw 419, thereby making the grabbing claw 419 move and penetrate into the transfer chamber 221. Then the user can control the grabbing hydraulic machine 4110 through the electrical control panel 102 to work, thereby making the two grabbing claws 419 move in the grabbing chamber 221. The side wall of the access rack 416 rotates, allowing the grasping claws 419 to move closer to each other and grasp the cell culture dish. Subsequently, the access servo motor 412, the vertical hydraulic device 415, and the telescopic hydraulic device 417 are controlled to operate, thereby allowing the cell culture dish to move three-dimensionally within the sealed box 1, and then the cell culture dish can be placed on the access rack 414. Repeating the above operation can sequentially place multiple cell culture dishes on the access rack 414, achieving the technical effect of automatically accessing cell samples in a sealed environment, and effectively solving the technical problem in the prior art that replacing cell samples will destroy the negative pressure sealed environment;

[0050] Similarly, take out the cell culture dish and process it in reverse order according to the above steps;

[0051] The user can calculate the pressure difference between the internal and external pressures of the sealed box 1 on the pressure-stabilizing piston 213 based on the area of one end of the pressure-stabilizing piston 213 located inside the sealed box 1 according to the required negative pressure for cell culture (this is a conventional pressure calculation method and will not be described in detail here). Then, based on this value, the pressure-stabilizing weight 219 can be placed on the pressure-stabilizing frame 217, and the gravity of the pressure-stabilizing weight 219 can be used to offset the pressure difference on the pressure-stabilizing piston 213 by transmitting force through the pressure-stabilizing rope 214. When the internal pressure is greater than the preset pressure, due to the reduction in the difference between the two ends of the pressure-stabilizing piston 213, the pressure-stabilizing piston 213 will move in the pressure-stabilizing groove 212 toward the outside of the sealed box 1 until it touches the pressure-stabilizing switch 218. Then, the negative pressure pump 211 is triggered to start, and the air in the sealed box 1 is pumped out, thereby reducing the internal pressure of the sealed box 1. When the internal pressure is lower than the preset pressure, due to the increase in the difference between the two ends of the pressure-stabilizing piston 213, the pressure-stabilizing piston 213 will move in the pressure-stabilizing groove 212 toward the inside of the sealed box 1 until it touches the pressure-stabilizing switch 218 on the other side of the pressure-stabilizing groove 212, thereby triggering the negative pressure pump 211 to turn off, thereby achieving the technical effect of automatically maintaining the internal pressure of the sealed box 1. At the same time, the number of pressure-stabilizing weights 219 can be replaced to adjust the internal pressure of the sealed box 1. At the same time, the pressure-stabilizing piston 213 is always in a dynamic equilibrium state, thereby reducing the pressure fluctuation inside the sealed box 1.

[0052] When the cell culture dish inside the sealed box 1 lacks culture fluid, the user can adjust the position of the grab frame 416, insert the injection needle 301 into the cell culture dish, and inject the culture fluid into the cell culture dish from the outside through the injection tube 302 and the injection needle 301 using the external culture fluid injection device, thereby achieving the technical effect of completing the filling of the cell culture dish while maintaining a sealed and negative pressure environment, effectively solving the technical problem of the prior art that requires re-pressure release and decompression, and avoiding cell contamination caused by the addition of nutrient solution;

[0053] During the cell culture process, the user can monitor the culture environment inside the sealed box 1 in real time through the temperature sensor 502 and the pressure sensor 501. At the same time, the internal image can be projected onto the external real-time screen 504 through the real-time camera 503, realizing all-round monitoring of the cell culture process and facilitating the user to grasp the cell culture progress in real time.

[0054] When it is necessary to sample and observe the cells in the cell culture dish in detail, the user can control the position of the grabbing frame 416 to insert the glass dropper 422 into the cell culture dish that needs to be sampled and observed, and control the pressing hydraulic machine 423 to extend and retract through the electrical control panel 102, so as to squeeze and restore the large end of the glass dropper 422 in the sampling groove 421, and suck out the cells in the cell culture dish, and transfer the cells to the outside through the transfer chamber 221 according to the above steps, thereby achieving the technical effect of sampling and observing cells without affecting other cell cultures, and effectively solving the technical effect of being unable to sample and observe in the existing technology.

[0055] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

[0056] The present invention and its embodiments are described above. This description is not restrictive. What is shown in the accompanying drawings is only one of the embodiments of the present invention. The actual structure is not limited to this. In short, if ordinary technicians in this field are inspired by it and do not depart from the purpose of the invention, they can creatively design structural methods and embodiments similar to the technical solution, which should all fall within the scope of protection of the present invention.

Claims

1. A negative pressure cell culture device, characterized in that: The invention comprises a sealed box (1), a negative pressure maintaining mechanism (2), a culture fluid injection mechanism (3), a cell access mechanism (4) and a monitoring mechanism (5), wherein the negative pressure maintaining mechanism (2) is arranged on the side wall of the sealed box (1), the culture fluid injection mechanism (3) is arranged inside the sealed box (1), the cell access mechanism (4) is arranged on the inner wall of the sealed box (1), and the monitoring mechanism (5) is arranged on the top of the inner wall of the sealed box (1). The negative pressure maintaining mechanism (2) comprises a pressure stabilizing mechanism (210) and a sealing mechanism ( 220), the pressure stabilizing mechanism (210) is arranged on the side wall of the sealed box (1), the sealing mechanism (220) is arranged on the side wall of the sealed box (1), the cell storage and access mechanism (4) comprises a cell dish storage and access mechanism (410) and a cell sampling mechanism (420), the cell dish storage and access mechanism (410) is arranged on the inner wall of the sealed box (1), the cell sampling mechanism (420) is arranged on the cell dish storage and access mechanism (410), and a storage rack (101) is fixedly provided on the inner bottom wall of the sealed box (1).

2. A negative pressure cell culture device according to claim 1, characterized in that: The pressure stabilizing mechanism (210) comprises a negative pressure pump (211), a pressure stabilizing groove (212), a pressure stabilizing piston (213), a pressure stabilizing rope (214), a pressure stabilizing seal ring (215), a pressure stabilizing wheel (216), a pressure stabilizing frame (217), a pressure stabilizing switch (218) and a pressure stabilizing weight (219), wherein the negative pressure pump (211) is fixedly arranged on the outer wall of the sealed box (1), the input end of the negative pressure pump (211) passes through the side wall of the sealed box (1), the pressure stabilizing groove (212) passes through the side wall of the sealed box (1), the pressure stabilizing piston (213) is opened, and the pressure stabilizing ring (215) is fixedly arranged on the outer wall of the sealed box (1). 13) is slidingly arranged on the inner wall of the pressure-stabilizing groove (212), the pressure-stabilizing sealing ring (215) is sleeved in pairs on the circumferential outer wall of the pressure-stabilizing piston (213), the pressure-stabilizing wheel (216) is rotatably arranged on the outer wall of the sealing box (1), one end of the pressure-stabilizing rope (214) is fixedly arranged on the side wall of the pressure-stabilizing piston (213), the top of the pressure-stabilizing frame (217) is fixedly arranged on the other end of the pressure-stabilizing rope (214), the pressure-stabilizing switch (218) is fixedly arranged in pairs on the inner wall of the pressure-stabilizing groove (212), and the pressure-stabilizing weight (219) is arranged on the pressure-stabilizing frame (217).

3. A negative pressure cell culture device according to claim 2, characterized in that: The sealing mechanism (220) includes a transfer chamber (221), an access door (222), a sealing door (223), an access rod (224), a sealing rod (225), a joint control arc block (226), a joint control groove 1 (227), a joint control groove 2 (228), a sealing spring 1 (229), a sealing spring 2 (2310), a sealing servo motor (2311), a sealing gear (2312), a sealing gear tooth (2313) and a sealing gasket (2314). The transfer chamber (221) is opened in the sealing box body (1). The sealing door (223) is rotatably mounted on one side wall of the transfer chamber (221) located in the sealed box body (1), the access door (222) is rotatably mounted on one side wall of the transfer chamber (221) located in the sealed box body (1), the sealing door (223) is communicated with the outside of the sealed box body (1), the sealing rod (225) is fixedly mounted on the side wall of the sealing door (223), the access rod (224) is fixedly mounted on the side wall of the access door (222), and the joint control arc block (226) is slidably mounted on the inner wall of the transfer chamber (221). The joint control groove 1 (227) is provided on the joint control arc block (226), the joint control groove 2 (228) is provided on the joint control arc block (226), the sealing rod (225) is slidably provided on the inner wall of the joint control groove 1 (227), the access rod (224) is slidably provided on the inner wall of the joint control groove 2 (228), one end of the sealing spring 1 (229) is fixedly provided on the side wall of the joint control groove 1 (227), the other end of the sealing spring 1 (229) is fixedly connected to the side wall of the sealing rod (225), and the sealing spring 2 (2310) is fixedly provided on the side wall of the joint control groove 1 (227). The end is fixedly arranged on the side wall of the second joint control groove (228), the other end of the second sealing spring (2310) is fixedly connected to the side wall of the access rod (224), the sealing servo motor (2311) is fixedly arranged on the inner wall of the transfer chamber (221), the sealing gear (2312) is coaxially fixedly arranged on the output end of the sealing servo motor (2311), the sealing gear (2313) array is fixedly arranged on the side wall of the joint control arc block (226), and the sealing gasket (2314) is fixedly arranged on the side walls of the access door (222) and the sealing door (223).

4. The negative pressure cell culture device according to claim 3, characterized in that: The cell dish access mechanism (410) comprises an access slot (411), an access servo motor (412), an access threaded rod (413), an access rack (414), a vertical hydraulic press (415), a grabbing rack (416), a telescopic hydraulic press (417), a telescopic rack (418), a grabbing claw (419) and a grabbing hydraulic press (4110), wherein the access slot (411) is provided on the inner wall of the sealed box (1), the access servo motor (412) is fixedly provided on the side wall of the access slot (411), the access threaded rod (413) is coaxially fixedly provided on the output end of the access servo motor (412), and the access rack (414) is slidably provided on the inner wall of the access slot (411). One end of the access threaded rod (413) passes through the side wall of the access frame (414), one end of the vertical hydraulic press (415) is fixedly arranged on the side wall of the access frame (414), the grabbing frame (416) is fixedly arranged on the output end of the vertical hydraulic press (415), the telescopic hydraulic press (417) is fixedly arranged on the side wall of the grabbing frame (416), the telescopic frame (418) is fixedly arranged on the output end of the telescopic hydraulic press (417), the grabbing claws (419) are rotatably arranged in pairs on the side wall of the telescopic frame (418), one end of the grabbing hydraulic press (4110) is rotatably arranged on the side wall of the telescopic frame (418), and the grabbing hydraulic press (4110) is rotatably connected to the side wall of the grabbing claws (419).

5. The negative pressure cell culture device according to claim 4, characterized in that: The cell sampling mechanism (420) comprises a sampling groove (421), a glass dropper (422) and a pressing hydraulic device (423), wherein the sampling groove (421) is provided on the telescopic frame (418), the glass dropper (422) is provided in the sampling groove (421), and the pressing hydraulic device (423) is fixedly provided on the inner wall of the sampling groove (421).

6. The negative pressure cell culture device according to claim 5, characterized in that: The culture fluid injection mechanism (3) comprises an injection needle (301) and an injection tube (302), wherein the injection needle (301) is fixedly arranged on the side wall of the telescopic frame (418), one end of the injection tube (302) is fixedly arranged on the top end of the injection needle (301), the injection needle (301) is communicated with the injection tube (302), and the other end of the injection tube (302) passes through the side wall of the sealed box (1).

7. The negative pressure cell culture device according to claim 6, characterized in that: The monitoring mechanism (5) comprises a pressure sensor (501), a temperature sensor (502), a real-time camera (503) and a real-time screen (504), wherein the pressure sensor (501) is fixedly arranged on the inner wall of the sealed box (1), the temperature sensor (502) is fixedly arranged on the inner wall of the sealed box (1), the real-time camera (503) is fixedly arranged on the inner wall of the sealed box (1), and the real-time screen (504) is fixedly arranged on the outer wall of the sealed box (1).

8. The negative pressure cell culture device according to claim 7, characterized in that: An electrical control panel (102) is fixedly provided on the outer wall of the sealed box (1), and the negative pressure pump (211), the sealing servo motor (2311), the access servo motor (412), the vertical hydraulic press (415), the telescopic hydraulic press (417), the grabbing hydraulic press (4110), the pressing hydraulic press (423), the pressure sensor (501), the temperature sensor (502), the real-time camera (503) and the real-time screen (504) are electrically connected to the electrical control panel (102) via wires.

9. The negative pressure cell culture device according to claim 8, characterized in that: The access threaded rod (413) is connected to the access frame (414) by a threaded connection, the sealing gear (2312) is meshed with the sealing gear teeth (2313), the linkage control groove 1 (227) and the linkage control groove 2 (228) are in an arc shape, the sealing door (223) and the access door (222) rotation axis coincide with each other, the arc centers of the linkage control groove 1 (227) and the linkage groove 2 coincide with the rotation axes of the sealing door (223) and the access door (222), and the sealing rod (225) and the access rod (224) are in an arc shape.

10. The negative pressure cell culture device according to claim 9, characterized in that: The inner side of the grabbing claw (419) is arc-shaped, and the large end of the glass dropper (422) is made of rubber.

Images