Rhizosphere soil sampling device and method

By designing a rhizosphere soil sampling device integrating camera module, LED light set and liquid supply and suction system, the problems of root damage and low sampling accuracy in the prior art are solved, and non-destructive and accurate soil sampling is achieved, which is suitable for agricultural science and environmental monitoring.

CN120489636AActive Publication Date: 2025-08-15INST OF AGRI RESOURCES & ENVIRONMENT SICHUAN ACAD OF AGRI SCI

Patent Information

Application Number
CN202510670665.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-23
Publication Date
2025-08-15
Estimated Expiration
2045-05-23

AI Technical Summary

Technical Problem

In the prior art, conventional sampling tools are prone to damage the plant roots during the sampling process, resulting in low sampling accuracy and insufficient operating efficiency, and inability to achieve in-situ observation or long-term continuous sampling.

Method used

An integrated visual, non-destructive rhizosphere soil sampling device is designed, including an operating handle, a snake bone tube and an end, equipped with a camera module, an LED light group, a liquid supply hole and aspiration hole. The sampling process is observed in real time through the display screen, and the flexibility and plasticity of the snake bone tube are used to adapt to different soil environments, and the precision sampling is carried out through the liquid supply and aspiration system.

Benefits of technology

Visualization and accurate sampling of rhizosphere soil is achieved, the root system is avoided, sampling accuracy and efficiency are improved, and it is suitable for agricultural science, plant ecology and environmental monitoring.

✦ Generated by Eureka AI based on patent content.

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Abstract

The rhizosphere soil sampling device comprises an operation handle and a sampling pipe, the front end of the end of the sampling pipe is a spherical surface, the surface of the sampling pipe is provided with an L-shaped notch, the L-shaped notch is provided with a first panel and a second panel, a camera module is integrated on the first panel, LED lamp sets are symmetrically arranged on the two sides of the camera module, and a liquid outlet hole is formed in the front side of the camera module; the liquid outlet hole is connected with a liquid supply pipe; the lens surface of the camera module is embedded in the first panel, so that direct contact or extrusion of soil is avoided; the second panel is perpendicular to the axis direction of the end, and a liquid suction hole is formed in the second panel and connected with a liquid suction pipe. The snake bone pipe comprises a plurality of joints which are hinged in sequence, and bending is achieved by controlling winding and unwinding of the steel wire rope through the direction operation rod. According to the device, the rhizosphere soil can be visually and accurately sampled, the root system is prevented from being damaged, and the sampling precision and efficiency are improved.
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Description

Technical Field

[0001] The present invention belongs to the field of soil sampling and relates to a device and method for rhizosphere soil sampling. The device and method are particularly suitable for non-destructive in-situ sampling of plant rhizosphere soil in the fields of agricultural science, plant ecology, and environmental monitoring. Background Art

[0002] Soil sampling is fundamental for studying plant-soil interactions, assessing soil fertility, and monitoring environmental pollution. The rhizosphere, the microenvironment where plant roots directly contact the soil, has a significant impact on plant growth through its physical and chemical properties and microbial activity.

[0003] Conventional sampling tools (such as soil drills and shovels) require digging up the soil and cutting roots, which damages plants and prevents in-situ observation or long-term continuous sampling. For example, mechanical drills can easily sever plant roots during operation, disrupting the rhizosphere microenvironment and affecting the accuracy of subsequent research. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide an integrated visual, non-destructive rhizosphere soil sampling device to solve the defects of traditional technologies such as damage to the root system, low sampling accuracy and insufficient operating efficiency.

[0005] The inventors have made continuous innovations and explorations through long-term exploration and experimentation, as well as numerous experiments and efforts. To solve the above technical problems, the present invention provides a rhizosphere soil sampling device, comprising: An operating handle, wherein the operating handle is provided with a display screen, a switch button assembly and a direction operating lever; The sampling tube includes a snake tube and an end head, wherein an optical fiber assembly, a liquid supply tube, a liquid suction tube, and multiple steel cables are integrated in the sampling tube; the front end of the end head is a spherical surface, and an L-shaped notch is provided on the surface thereof, wherein the L-shaped notch has a first panel and a second panel, wherein: The angle between the first panel and the axis of the end head is 170° to 180°, and the camera module is integrated thereon. The LED light groups are symmetrically arranged on both sides of the camera module, and a liquid outlet is arranged on the front side, which is connected to the liquid supply pipe. The lens surface of the camera module is embedded in the first panel to avoid direct contact or squeezing with the soil. The second panel is perpendicular to the axis of the end head and is provided with a liquid suction hole connected to the liquid suction tube; The snake tube comprises a plurality of sequentially hinged joints, and the bending is achieved by controlling the retraction and extension of the steel wire rope through a direction operating lever.

[0006] Compared with the prior art, the present invention has the following beneficial effects: The device enables visualization and precise sampling of rhizosphere soil, avoids damage to the root system, and improves sampling accuracy and efficiency. First, through the display screen and switch button assembly on the operating handle, the user can observe images and data during the sampling process in real time and conveniently control various functions of the device, such as lighting, photo and video recording, image zooming, and sample collection, to achieve precise control of the sampling process. Secondly, the snake tube and end of the sampling tube are uniquely designed. The multiple articulated joints of the snake tube give it good flexibility and plasticity, allowing it to adapt to different soil environments and root distributions. The camera module, LED light group, liquid outlet and liquid suction holes on the end enable visualization and precise sampling of rhizosphere soil, ensuring that representative soil samples are collected. In addition, the coordinated use of the liquid supply and liquid suction systems can effectively collect rhizosphere soil samples. At the same time, the anti-backflow valve design prevents liquid backflow, ensuring the smooth progress of the sampling process. In general, the rhizosphere soil sampling device of the present invention has significant advantages in protecting the root system and improving sampling accuracy and efficiency, and is suitable for soil sampling work in the fields of agricultural science, plant ecology and environmental monitoring.

[0007] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the lens and LED light group of the camera module are covered with wear-resistant transparent material.

[0008] Preferably, the wear-resistant transparent material is glass.

[0009] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: Using wear-resistant transparent materials (such as glass) to cover the lens and LED light group of the camera module can effectively protect the lens and light group from soil wear, while maintaining good optical performance and ensuring image clarity and fill light effect.

[0010] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the size of the camera module is no more than 3.0mm×3.0mm×2.5mm, and its resolution is ≥400×400 pixels.

[0011] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The size of the camera module is controlled to no more than 3.0mm×3.0mm×2.5mm and the resolution is no less than 400×400 pixels, so that the end of the sampling device can more flexibly shuttle and operate in the narrow rhizosphere space, effectively improving the adaptability to rhizosphere soil and sampling flexibility; at the same time, the high-resolution camera module ensures the clarity and details of the transmitted images, providing a strong guarantee for accurately determining the root position and controlling the sampling process, thereby significantly improving the accuracy and success rate of rhizosphere soil sampling.

[0012] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: a damping member is provided between each joint of the snake tube, the elastic modulus of the damping member is 0.5~2MPa, and when the joint bending angle range is ≤60°, the deformation recovery rate of the damping member is ≥95%.

[0013] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: By setting a damping member with an elastic modulus of 0.5~2MPa between each joint of the serpentine tube and ensuring that the deformation recovery rate of the damping member is ≥95% when the joint is in a bending angle range of ≤60°, the serpentine tube can have good flexibility and stability during bending and movement, effectively improving its control accuracy and adaptability in complex soil environments, while preventing equipment damage caused by excessive bending and extending its service life.

[0014] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the liquid supply pipe and the liquid suction pipe are respectively connected to the liquid supply pump and the liquid suction pump, the flow adjustment range of the liquid supply pump is 0.1~5mL / s, the suction negative pressure of the liquid suction pump is -10~-50kPa, and both are equipped with anti-backflow valves.

[0015] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The flow rate and negative pressure adjustment range design of the liquid supply pump and the liquid suction pump, combined with the anti-backflow valve, can accurately control soil moistening and sample collection rate, prevent sample contamination, and improve sampling efficiency and accuracy.

[0016] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the switch button assembly includes: a power switch, a lighting switch, a photo button, a video button, an image zoom button, an image zoom button, and a sampling button.

[0017] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: By integrating multiple function buttons, the device achieves convenient and precise control of the sampling process, meets diverse operational needs, and significantly improves user experience and sampling efficiency.

[0018] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the sampling button is used to trigger the start and stop of the liquid supply pump and the liquid suction pump. The liquid suction pump starts later than the liquid supply pump, and the delay time is set to 0.1~1s.

[0019] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: This design can precisely control the sampling process, ensuring the accuracy and timing of rhizosphere soil moisture and sample extraction, reducing the potential risk of damage to the root system, while improving the sampling success rate and operational convenience.

[0020] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the ejection direction of the liquid outlet intersects with the propulsion direction of the end head; the liquid suction hole is an inclined conical hole, the axis of which forms an angle of 30°~60° with the second panel, and the edge of the hole is mirror polished.

[0021] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The spray direction of the liquid outlet intersects with the propulsion direction of the end head, which can effectively moisten the soil around the target root system. The design of the inclined conical suction hole helps to accurately suck the rhizosphere soil solution. The mirror-polished edge of the hole can reduce soil adhesion. The combination of the two can improve the sampling accuracy and efficiency while reducing the disturbance to the soil and root system.

[0022] On the basis of the above technical solution, the present invention can also be improved as follows: Furthermore: the optical wire group includes a power line, a signal transmission line and a control line.

[0023] Compared with the prior art, the beneficial effects of adopting the above further technical solution are: The integrated design of the optoelectronic cable group ensures the stable operation of the sampling device in complex soil environments, realizes efficient coordination of power supply, image signal transmission and operation control, and greatly improves the accuracy and convenience of the sampling process.

[0024] The present invention also provides a rhizosphere soil sampling method, based on the aforementioned device, comprising the following steps: (a) According to the root distribution range of the target plant, operate the direction joystick to control the snake tube to slowly insert into the soil; (b) During the insertion process, the image transmitted by the camera module is observed on the display screen, and the LED light group is turned on for fill light. The bending angle of the snake tube is adjusted according to the root position so that the tip is close to the target root system; (c) When the tip reaches the root system, ensure that the outlet is facing the root. Press the sampling button to start the liquid supply pump, and deliver the solution through the outlet to moisten the soil. The wetting time is adjusted according to the dryness of the soil until it is moist without water accumulation. The suction pump starts after a delay of 0.1 to 1 second and extracts the rhizosphere soil solution through the suction hole. (d) Control the snake tube along the root growth direction, monitor the negative pressure fluctuation in real time during the suction process, and automatically stop and alarm if it exceeds ±5kPa; (e) Adjust the suction force according to the image on the display screen to ensure that the solution flows steadily and does not damage the root system; (f) After sampling is completed, the snake tube is withdrawn.

[0025] Compared with the prior art, the present invention has the following beneficial effects: The present invention controls the snake tube to slowly insert into the soil by operating the direction joystick, and uses the display screen to observe the image transmitted by the camera module, turns on the LED light group to fill in the light, and can accurately adjust the snake tube bending angle according to the root position so that the end is close to the target root system. This visual operation and precise control capability greatly improves the sampling accuracy and avoids interference and damage to non-target roots. Secondly, when the end reaches the root system, by reasonably setting the direction and shape of the liquid outlet and the liquid suction hole, and using the coordinated work of the liquid supply pump and the liquid suction pump, the solution is first transported to moisten the soil, and then the liquid suction pump is delayed to extract the rhizosphere soil solution, effectively ensuring the stable flow and accurate collection of the soil solution, while avoiding the sampling difficulties and sample contamination caused by dry or over-wetting of the soil. Furthermore, the negative pressure fluctuations are monitored in real time during the suction process. If the set threshold is exceeded, it automatically stops and alarms, and the suction force is adjusted according to the display screen image to ensure that the solution flows stably and does not damage the root system. These intelligent monitoring and feedback mechanisms further improve the reliability of sampling and the safety of the root system. Finally, the snake tube is retracted after sampling is completed. The whole process is efficient and orderly. It can not only obtain representative and accurate rhizosphere soil samples, but also minimize the damage to the root system and surrounding soil environment, providing strong support for subsequent soil research and ecological analysis. It is especially suitable for soil sampling work in the fields of agricultural science, plant ecology and environmental monitoring. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying any creative work.

[0027] Figure 1 It is a front structural schematic diagram of a preferred embodiment of the rhizosphere soil sampling device of the present invention.

[0028] Figure 2 yes Figure 1 Schematic diagram of the three-dimensional structure.

[0029] Figure 3 It is a schematic diagram of the partial structure of the snake tube and the end in a preferred embodiment of the rhizosphere soil sampling device of the present invention.

[0030] Figure 4 yes Figure 3 Schematic diagram of the three-dimensional structure.

[0031] Figure 5 yes Figure 3 Another three-dimensional structural schematic diagram of .

[0032] Figure 6 It is a schematic diagram of the partial structure of the snake tube and the end in another preferred embodiment of the rhizosphere soil sampling device of the present invention.

[0033] The marks in the figure are: 100 operating handle, 110 display screen, 120 switch button assembly, 130 direction joystick, 200 sampling tubes, 210 snake bone tube, 211 damping parts, 212 guide ring, 220 terminal, 201 First Panel, 202 Second Panel, 221 camera module, 222LED light group, 223 liquid outlet, 224 suction holes, 230 optical wire group, 240 liquid supply pipe, 250 pipettes, 260 steel wire rope, 270 casing. DETAILED DESCRIPTION

[0034] The following describes a specific embodiment with reference to the accompanying drawings.

[0035] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention.

[0036] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it may not be further defined or explained in subsequent drawings.

[0037] Example 1 See also Figures 1 to 6 The rhizosphere soil sampling device described in this embodiment has the following specific structure and composition: The operating handle 100 is the operating center of the entire device, and a display screen 110 is integrated thereon, which can clearly display the images and data sent back by the camera module 221, making it convenient for the operator to observe the sampling situation in real time.

[0038] In a further embodiment, the display screen 110 can be a touch screen display screen 110. Such a design makes the operation interface more intuitive and convenient. The operator can perform various operations directly on the display screen 110, such as touching and clicking to control the photo and video functions of the camera module 221, or adjusting the image size by gesture zooming to more clearly view the root details and soil conditions. In addition, the touch screen display screen 110 can also be integrated with virtual buttons to replace some physical buttons, further simplifying the structure of the operating handle 100, improving the overall aesthetics and waterproof performance of the device, and providing the operator with a more flexible and efficient operating experience.

[0039] The switch button assembly 120 includes a power switch, a lighting switch, a photo button, a video button, an image zoom button, an image zoom button, and a sampling button. These buttons allow the operator to conveniently control various functions of the device. The direction control lever 130 is used to control the bending direction of the snake tube 210, thereby adjusting the position and posture of the sampling tube 200 in the soil.

[0040] The power switch is used to control the power on and off of the entire device.

[0041] The lighting switch controls the on and off of the LED light groups 222 on both sides of the camera module 221. In a dark soil environment, by turning on the lighting switch, sufficient fill light is provided for the camera module 221 to ensure that the captured image is clear and visible, which facilitates the operator to accurately judge the root position and the surrounding soil conditions.

[0042] The photo button is used to capture still images during the sampling process. When you need to record key information such as root morphology, soil conditions, or sampling location at a specific moment, pressing this button will take a high-resolution photo, providing intuitive visual data for subsequent research and analysis.

[0043] The video button functions similarly to the photo button, but it also records dynamic video. During operation, if you need to observe dynamic changes in the root system, observe solution flow, or record the entire sampling process, pressing the video button will activate video recording, providing richer dynamic information for studying the rhizosphere soil environment.

[0044] The image magnification button can magnify the image displayed on the display screen 110, making it easier for the operator to observe detailed information such as the microstructure of the root system, the distribution of soil particles, and the relative position of the end 220 and the root system in more detail, thereby more accurately controlling the movement and sampling operation of the snake tube 210 and improving the sampling accuracy.

[0045] The image zoom button has the opposite function to the image zoom button and is used to zoom out the image on the display screen 110. When it is necessary to view the entire sampling area as a whole, or to return to the normal field of view after zoomed-in observation to adjust the macroscopic position of the snake tube 210, pressing this button quickly zooms out the image, helping the operator to fully grasp the sampling environment.

[0046] The sampling button is the key trigger button for the sampling operation. When pressed, it will start the liquid supply pump (not shown in the figure) and the liquid suction pump (not shown in the figure) in sequence, realizing the automated process of delivering moistening solution to the rhizosphere soil and extracting samples, effectively simplifying the operating steps, improving sampling efficiency, and ensuring the accuracy and stability of the sampling process.

[0047] See also Figures 3-5 The sampling tube 200 includes a snake tube 210 and an end 220 at the front end of the tube, and is a key component for achieving soil sampling.

[0048] The snake tube 210 consists of multiple sequentially hinged joints. A guide ring 212 for a limited position steel wire rope 260 is located within the snake tube 210. A damper 211 is located between each joint. The elastic modulus of damper 211 is 0.5-2 MPa. When the joint bending angle range is ≤60°, the deformation recovery rate of damper 211 is ≥95%. This design gives the snake tube 210 excellent flexibility and stability, allowing it to flex and move flexibly in complex soil environments. During operation, it maintains a stable posture and is not damaged by excessive bending. The sampling tube 200 integrates an optical cable assembly 230, a liquid supply tube 240, a liquid suction tube 250, and two or four steel wire ropes 260. The optical cable assembly 230 includes power lines, signal transmission lines, and control lines. Its outer layer is coated with corrosion-resistant insulating material, providing power and signal transmission to components such as the camera module 221. Its high strength and corrosion resistance ensure reliable operation in soil environments. Its three-dimensional spatial motion control refers to the endoscope. The operator can accurately control the bending direction of the snake tube 210 through the direction operating rod 130, so that the sampling tube 200 can smoothly reach the target root position and can flexibly shuttle even in narrow or complex soil spaces, providing strong support for rhizosphere soil sampling.

[0049] End cap 220: The front end is spherical, which is convenient for soil application and avoids damage to plant roots. An L-shaped notch is provided on its side to form a first panel 201 and a second panel 202. The angle between the first panel 201 and the axis of the end cap 220 is 170°~180°. Figure 3The diagram shows a situation where the axis of the first panel 201 and the end head 220 forms an angle of 180°. A camera module 221 is integrated into the first panel 201. The dimensions of the camera module 221 are no larger than 3.0 mm x 3.0 mm x 2.5 mm, with a resolution of 400 x 400 pixels or higher. This allows for clear images of the soil's interior, providing operators with accurate visual information. Micro camera sensor modules such as the OVM9284, OVM6946, IMX225, IMX178, AR0134CS, AR1335, VD6281, CMV2000, and GalaxyCore GC2145 are all available. LED light groups 222 are symmetrically positioned on either side of the camera module 221 to provide supplemental illumination for the camera module 221 in dimly lit soil environments, ensuring image clarity. A liquid outlet 223 is provided on the front side. The spray direction of the liquid outlet 223 intersects with the propulsion direction of the end head 220, which can evenly spray the solution into the soil around the target root system. The liquid outlet 223 is connected to the liquid supply pipe 240. The liquid supply pump can transport the solution to the liquid outlet 223 through the liquid supply pipe 240 to moisten the soil. The second panel 202 is perpendicular to the axis of the end head 220 and is provided with a liquid suction hole 224. The liquid suction hole 224 is an inclined conical hole. Its axis forms an angle of 30° to 60° with the second panel 202. The edge of the hole is mirror-polished, which can effectively extract the rhizosphere soil solution and reduce soil adhesion to the hole, thereby improving sampling efficiency. The liquid suction hole 224 is connected to the liquid suction pipe 250. The liquid suction pump can use the liquid suction pipe 250 to extract the rhizosphere soil solution into the sample container (not shown in the figure).

[0050] In a further embodiment, the lens of the camera module 221 and the LED light group 222 are covered with a wear-resistant transparent material. Preferably, the wear-resistant transparent material is glass or organic glass. Glass has good transparency and wear resistance, and can effectively protect the lens of the camera module 221 and the LED light group 222 from wear and scratches caused by soil particles, ensuring that the lens and the light group can work stably for a long time in a complex soil environment. At the same time, the high transparency of the glass material ensures that the camera module 221 can clearly capture the image inside the soil, and the image quality will not be affected by wear or contamination of the material. The LED light group 222 can also maintain a stable brightness output under the protection of the glass, providing reliable fill light for the camera module 221, ensuring that a clear image can still be obtained in a darker soil environment. This design not only improves the service life of the camera module 221, but also enhances its reliability and stability during the soil sampling process, providing a strong guarantee for accurate rhizosphere soil sampling.

[0051] Liquid supply and aspiration system: Liquid supply tube 240 is connected to a liquid supply pump (not shown), and a liquid aspiration tube 250 is connected to a liquid aspiration pump (not shown). The flow rate adjustment range of the liquid supply pump is 0.1 to 5 mL / s, and the suction pressure of the liquid aspiration pump is -10 to -50 kPa. Both are equipped with anti-backflow valves to effectively prevent liquid backflow and ensure smooth liquid supply and aspiration processes. During sampling, the liquid supply pump first delivers a solution (preferably sterile distilled water) to the liquid outlet 223 to moisten the soil. The aspiration pump then starts after a delay of 0.1 to 1 second, which can be adjusted based on soil moisture. The rhizosphere soil solution is then extracted through the aspiration hole 224. This design ensures a stable flow of soil solution and avoids sample contamination or sampling failure due to improper timing of liquid supply and aspiration.

[0052] See also Figure 6 A sleeve 270 is also provided outside the sampling tube 200, and its main function is to provide more comprehensive protection for the sampling tube.

[0053] The sampling method described in this embodiment is that the operator controls the snake tube 210 by operating the direction control lever 130 to slowly insert it into the soil. During the insertion process, the operator observes the image transmitted by the camera module 221 through the display screen 110, and turns on the LED light group 222 for supplementary lighting. The bending angle of the snake tube 210 is adjusted according to the root position, so that the end 220 approaches the target root system. When the end 220 reaches the root system, the liquid outlet 223 is ensured to be oriented in the direction of the roots. The sampling button is pressed to start the liquid supply pump, which delivers solution through the liquid outlet 223 to moisten the soil. The wetting time is adjusted according to the dryness of the soil until it is moist and there is no water accumulation. The liquid suction pump starts after a delay of 0.1 to 1 second and extracts the rhizosphere soil solution through the liquid suction hole 224. The snake tube 210 is controlled along the direction of root growth. During the suction process, the negative pressure fluctuation is monitored in real time. If it exceeds ±5kPa, it automatically stops and an alarm is issued. The suction force is adjusted according to the image on the display screen 110 to ensure that the solution flows steadily and does not damage the root system. After sampling is completed, the snake tube 210 is withdrawn. The rhizosphere soil sampling device provided in this embodiment can achieve accurate and non-destructive sampling of rhizosphere soil, providing strong support for research in fields such as agricultural science, plant ecology, and environmental monitoring.

[0054] After completing a sampling, the sampling tube 200 needs to be replaced, or the sampling tube 200, especially the pipette 250, needs to be thoroughly cleaned with sterile water to avoid cross contamination of microorganisms.

[0055] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like to indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0056] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature identified as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

[0057] In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," "connect," "fixed," etc. should be understood broadly. For example, they may refer to fixed connection, detachable connection, or integration; mechanical connection or electrical connection; direct connection or indirect connection through an intermediate medium; internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0058] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0059] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be construed as limiting the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. Persons skilled in the art will appreciate that improvements and modifications may be made without departing from the spirit and scope of the present invention, and such improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A rhizosphere soil sampling device, characterized in that: include: An operating handle, wherein the operating handle is provided with a display screen, a switch button assembly and a direction operating lever; The sampling tube includes a snake tube and an end head, wherein an optical fiber assembly, a liquid supply tube, a liquid suction tube, and multiple steel cables are integrated in the sampling tube; the front end of the end head is a spherical surface, and an L-shaped notch is provided on the surface thereof, wherein the L-shaped notch has a first panel and a second panel, wherein: The angle between the first panel and the axis of the end head is 170° to 180°, and the camera module is integrated thereon. The LED light groups are symmetrically arranged on both sides of the camera module, and a liquid outlet is arranged on the front side, which is connected to the liquid supply pipe. The lens surface of the camera module is embedded in the first panel to avoid direct contact or squeezing with the soil. The second panel is perpendicular to the axis of the end head and is provided with a liquid suction hole connected to the liquid suction tube; The snake tube comprises a plurality of sequentially hinged joints, and the bending is achieved by controlling the retraction and extension of the steel wire rope through a direction operating lever.

2. The rhizosphere soil sampling device according to claim 1, characterized in that: The lens and LED light group of the camera module are covered with wear-resistant transparent material.

3. The rhizosphere soil sampling device according to claim 1 or 2, characterized in that: The size of the camera module is no larger than 3.0mm×3.0mm×2.5mm, and its resolution is ≥400×400 pixels.

4. The rhizosphere soil sampling device according to claim 1, characterized in that: A damping member is provided between each joint of the serpentine tube. The elastic modulus of the damping member is 0.5-2 MPa, and when the joint bending angle range is ≤60°, the deformation recovery rate of the damping member is ≥95%.

5. The rhizosphere soil sampling device according to claim 1, characterized in that: The liquid supply pipe and the liquid suction pipe are respectively connected to the liquid supply pump and the liquid suction pump. The flow adjustment range of the liquid supply pump is 0.1~5mL / s, the suction negative pressure of the liquid suction pump is -10~-50kPa, and both are equipped with anti-backflow valves.

6. The rhizosphere soil sampling device according to claim 1, characterized in that: The switch button assembly includes: a power switch, a lighting switch, a photo button, a video button, an image zoom button, an image reduction button, and a sampling button.

7. The rhizosphere soil sampling device according to claim 6, characterized in that: The sampling button is used to trigger the start and stop of the liquid supply pump and the liquid suction pump. The liquid suction pump starts later than the liquid supply pump, and the delay time is set to 0.1~1s.

8. The rhizosphere soil sampling device according to claim 1, characterized in that: The ejection direction of the liquid outlet intersects with the propulsion direction of the end head; the liquid suction hole is an inclined conical hole, the axis of which forms an angle of 30° to 60° with the second panel, and the edge of the hole is mirror polished.

9. The rhizosphere soil sampling device according to claim 1, characterized in that: The optical wire assembly includes a power line, a signal transmission line and a control line.

10. A rhizosphere soil sampling method, based on the device according to any one of claims 1 to 9, characterized in that: The following steps are involved: (a) According to the root distribution range of the target plant, operate the direction joystick to control the snake tube to slowly insert into the soil; (b) During the insertion process, the image transmitted by the camera module is observed on the display screen, and the LED light group is turned on for fill light. The bending angle of the snake tube is adjusted according to the root position so that the tip is close to the target root system; (c) When the tip reaches the root system, ensure that the outlet is facing the root. Press the sampling button to start the liquid supply pump, and deliver the solution through the outlet to moisten the soil. The wetting time is adjusted according to the dryness of the soil until it is moist without water accumulation. The suction pump starts after a delay of 0.1 to 1 second and extracts the rhizosphere soil solution through the suction hole. (d) Control the snake tube along the root growth direction, monitor the negative pressure fluctuation in real time during the suction process, and automatically stop and alarm if it exceeds ±5kPa; (e) Adjust the suction force according to the image on the display screen to ensure that the solution flows steadily and does not damage the root system; (f) After sampling is completed, the snake tube is withdrawn.

Citation Information

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