Method for detecting zearalenone, aflatoxin and vomitoxin based on xMAP technology

By optimizing the antigen coating and antibody concentration of xMAP technology and combining with high-throughput analysis instruments, high sensitivity and specific detection of zearalenone, aflatoxin and vomiting toxin are achieved, solving the problem of not identifying high-risk samples without toxicity in the prior art, and improving the efficiency of food safety detection.

CN120490465APending Publication Date: 2025-08-15ZHEJIANG UNIV +1
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Patent Information

Application Number
CN202510643822.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-05-19
Publication Date
2025-08-15

AI Technical Summary

Technical Problem

The existing mycotoxin detection methods cannot effectively identify high-risk samples that do not produce toxins, resulting in the inability to detect mold contamination in time, and there are food and feed safety risks.

Method used

Using a competitive immune assay method based on xMAP technology, the working concentration of antigen coating, detection of antibodies and signal amplification antibodies is optimized, and the simultaneous detection of zearalenone, aflatoxin and vomiting toxin are achieved by optimizing the working concentration of antigen coating, detection of antibodies and signal amplification antibodies, and combining high-throughput analysis instruments.

Benefits of technology

It significantly improves the sensitivity and specificity of the detection, reduces the sample collection and processing steps, shortens the detection time, reduces the cost, and provides an efficient and economical food safety testing solution.

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Abstract

The invention belongs to the technical field of mould detection, and discloses a zearalenone, aflatoxin and vomitoxin detection method based on an xMAP technology, and the specific steps are as follows: S1, establishment of an antigen coating of the zearalenone xMAP detection method: coating a purified antigen with magnetic beads by using # imgabs0 # Antibody Coupling Kit; by utilizing the multi-labeling characteristic of the fluorescent encoding microspheres, multiple target molecules can be simultaneously identified in a single reaction, so that the sample collection quantity and processing steps are greatly reduced, and the tedious process of repeated experiments in a traditional method is avoided; by optimizing key parameters such as antigen coating conditions, working concentration of detection antibodies and signal amplification antibodies and the like, the sensitivity is remarkably improved on the premise of ensuring detection specificity, and meanwhile, a competitive immune test mode is combined with a high-throughput analysis instrument, so that single-time detection time is greatly shortened, the use amount of samples is extremely small, complex pretreatment is not needed, and the detection sensitivity is greatly improved. The operation process is standardized, simple and fast, and special equipment maintenance is not needed.
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