Anti-aging plant essence and use thereof

Abstract

The present application provides an anti-aging plant essence and its application, belonging to the field of medical, dental or cosmetic preparations. The components of the present application include tripeptide-1, palmitoyl pentapeptide-4, turmeric root extract, white plumeria flower extract and hosta extract. The present application innovatively enhances the activity of plant extract through bio-fermentation process, combines polypeptide targeted repair with the synergistic mechanism of multi-channel anti-oxidation and anti-inflammation of plant extract, and realizes multi-dimensional coverage of anti-aging effect; at the same time, the freeze-dried powder form and the antioxidant system ensure the stability of the product, providing an advanced solution for anti-aging product development with strong bioactivity and user-friendly.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of medical, dental or cosmetic formulations, in particular to an anti-aging plant essence and its application. BACKGROUND

[0002] With the rapid development of technology and the continuous improvement of living quality, people's demand for health and maintenance is growing. Oxidative stress, as a mechanism closely related to many diseases and aging processes, has received extensive attention in recent years. The research and application of antioxidants have become a popular field, among which natural plant extracts have become an important direction of antioxidant research due to their rich diversity, small side effects and wide sources. With the in-depth study of natural plant chemical components, it is found that many plant extracts are rich in antioxidant active substances such as polyphenols and flavonoids, which have the ability to scavenge free radicals and inhibit oxidation.

[0003] There is a direct and close causal relationship between antioxidant and skin anti-aging. Oxidative stress is the core driving factor of skin aging, and antioxidant mechanism is the key defense line against this process. Skin aging is the result of multiple factors such as oxidative damage, collagen loss and inflammatory response. Therefore, to achieve the effect of skin anti-aging, multiple paths need to be combined. SUMMARY

[0004] The purpose of the present application is to provide an anti-aging plant essence and its application, which achieves excellent anti-aging effect through multiple synergistic effects, including the following ingredients:

[0005] The following ingredients are included by mass fraction:

[0006]

[0007] The turmeric root extract is the product obtained by purifying the fermentation product of turmeric root by Lactobacillus rhamnosus;

[0008] The white Plumeria alba flower extract is the product obtained by purifying the fermentation product of white Plumeria alba flower by Aspergillus oryzae.

[0009] Preferably, the preparation method of the turmeric root extract is: mixing the activated Lactobacillus rhamnosus with the turmeric root fermentation substrate, then anaerobic fermentation, centrifugal separation, concentration and purification after fermentation, and drying;

[0010] The turmeric root fermentation substrate contains turmeric root, auxiliary carbon source and water.

[0011] The anaerobic fermentation conditions are:

[0012] 35-38℃, pH 6.0-7.0; fermentation for at least 72h.

[0013] Preferably, the preparation method of the white Plumeria alba flower extract is as follows: mixing activated Aspergillus oryzae with Plumeria alba flower fermentation substrate, then performing aerobic solid-state fermentation, centrifuging, concentrating and purifying after the fermentation is completed, and drying.

[0014] The Plumeria alba flower, the auxiliary carbon source and water in the Plumeria alba flower fermentation substrate.

[0015] The conditions for aerobic solid-state fermentation are as follows:

[0016] 28-32 ℃, pH 6.5-6.8; and the fermentation is performed for at least 60 h.

[0017] Preferably, the anti-aging plant essence further comprises an antioxidant; the antioxidant is at least one of vitamin E, butylated hydroxytoluene and edifen.

[0018] Preferably, the dosage form of the anti-aging plant essence is freeze-dried powder.

[0019] When the anti-aging plant essence contains an antioxidant, a freeze-drying protective agent is further included.

[0020] The application further provides use of the aforementioned anti-aging plant essence in the preparation of cosmetics; including but not limited to dosage forms such as serum, cream, emulsion, freeze-dried powder and the like.

[0021] The minimum addition amount of the anti-aging plant essence in the preparation of cosmetics is 0.1 wt%.

[0022] The application realizes multi-channel synergistic anti-aging, mainly including:

[0023] The two signal peptides of tripeptide-1 and palmitoyl pentapeptide-4 are compounded; the molecular weight of tripeptide-1 is smaller, which can penetrate into the dermis layer, activate fibroblasts to promote the synthesis of collagen and elastic fibers, and improve skin relaxation and wrinkles. Palmitoyl pentapeptide stimulates the regeneration of type I and type IV collagen by simulating collagen fragments. Since the effective concentration of peptides is very low, a small addition amount can achieve good results.

[0024] Alchemilla vulgaris extract inhibits elastase activity, reduces wrinkle formation, and at the same time relieves sensitive skin through anti-inflammatory soothing.

[0025] The white Plumeria alba flower extract is rich in polyphenolic compounds and flavonoids, has the effects of neutralizing oxidative damage and delaying skin aging, and promoting collagen synthesis.

[0026] Turmeric root extract contains high-activity curcumin, inhibits inflammatory factors (TNF-α, IL-1β) and lipid peroxidation, and reduces photoaging damage.

[0027] The present application selects specific strains for fermentation in order to increase the beneficial effects of white egg flower and turmeric root; white egg flower is subjected to solid aerobic fermentation by Aspergillus oryzae to decompose alkaloids that may cause allergies while releasing more active polysaccharides and flavonoids and the like. Similarly, the secondary metabolites generated by fermentation of turmeric root by Lactobacillus rhamnosus are subjected to effect optimization.

[0028] The present application finds that not all strains of fermentation can bring better beneficial effects, which depends on the fermentation strain, process control and the characteristics of the target active ingredient; for example, if the target product happens to be a nutrient required by the strain metabolism, it will instead lead to a decrease in the beneficial effect; or the process is not properly adapted, such as temperature, oxygen, pH range, etc.

[0029] The beneficial technical effects of the present application are at least as follows:

[0030] The present application innovatively enhances the activity of plant extracts through a biological fermentation process, realizes multi-dimensional coverage of anti-aging effects by combining polypeptide targeted repair with the synergistic mechanism of multi-pathway antioxidant and anti-inflammatory of plant extracts, and at the same time ensures product stability with a freeze-dried powder dosage form and an antioxidant system, thereby providing an advanced solution for anti-aging product development with both biological activity enhancement and user friendliness. DETAILED DESCRIPTION

[0031] In order to better understand the present application, the present application will be further described below in combination with specific serial numbers, wherein the terms used in the serial numbers are used to describe specific embodiments and do not constitute a limitation on the scope of protection of the present application.

[0032] In the detailed description, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents and the like used are commercially available unless otherwise specified.

[0033] Unless otherwise specified, the percentages, % and the like in the detailed description are by mass percentage.

[0034] Some of the raw materials used in the present application are as shown in Table 1 below:

[0035] Table 1

[0036] Name INCI Name / Latin Name Tripeptide-1 Tripeptide-1 Palmitoyl pentapeptide-4 Palmitoyl pentapeptide-4 Curcuma longa root extract Curcuma longa root extract Plumeria alba flower extract Plumeria alba flower extract Lactobacillus plantarum L. plantarum Lactobacillus rhamnosus L. rhamnosus Bacillus subtilis B. subtilis Aspergillus oryzae A. oryzae

[0037] Example 1

[0038] The preparation method of the fermented white egg flower extract includes the following steps:

[0039] S1: Take fresh white egg flower petals, remove the calyx and peduncle, rinse with water and drain; break into 0.5-1 cm pieces;

[0040] S2: The substrate is prepared according to the proportion in Table 2, and appropriate amount of sterile water is added to adjust the moisture content;

[0041] S3: The activated bacterial strain is prepared into a 1 x 10 7 -1 x 10 8 CFU / mL suspension, inoculated at a proportion of 5%, and then cultured according to the conditions in Table 2;

[0042] S4: After the culture is completed, inactivation treatment is performed, and the supernatant is taken after high-speed centrifugation; the supernatant is filtered using a nanofiltration membrane (NF) to take the filtrate, which is then freeze-dried and stored in a sealed manner.

[0043] Table 2

[0044]

[0045] Example Two

[0046] The preparation method of the fermented turmeric root extract includes the following steps:

[0047] S1: Fresh turmeric roots are taken, washed, and sliced (thickness ≤0.5 cm), and dried at a low temperature of 60°C until the moisture content is ≤10%; then, the sliced roots are crushed through a 60-mesh sieve to obtain turmeric root powder.

[0048] S2: The substrate is prepared according to the proportion in Table 3, and appropriate amount of sterile water is added to adjust the moisture content;

[0049] S3: The activated bacterial strain is prepared into a 1 x 10 7 -1 x 10 8 CFU / mL suspension, inoculated at a proportion of 5%, and then cultured according to the conditions in Table 3;

[0050] S4: After the culture is completed, inactivation treatment is performed at 60°C, and the supernatant is taken after high-speed centrifugation; the supernatant is first filtered using a microfiltration membrane (MF) to take the filtrate, and then filtered using a nanofiltration membrane (NF) to take the filtrate, which is then freeze-dried and stored in a sealed manner.

[0051] Table 3

[0052]

[0053]

[0054] Example Three

[0055] The antioxidant performance of the plant extract is tested; the test method is as follows:

[0056] The DPPH methanol solution is violet, and has a strong absorbance value at 517 nm; if combined with the sample, the absorbance value at 517 nm will decrease, thereby determining the ability of the sample to scavenge DPPH free radicals.

[0057] The specific method is as follows:

[0058] (1) Take the samples of Example 1, Example 2 and commercially available samples, mix with sterile water to prepare 0.5% concentration of equal volume (2 mL) of the test solution and 2 x 10 -4 mol / L DPPH solution (A1);

[0059] (2) Take equal volume of anhydrous ethanol (solvent of the test substance) and 2 x 10 -4 mol / L DPPH solution (A2);

[0060] (3) Take equal volume of anhydrous ethanol and the test solution (A3);

[0061] (4) After 40 min of reaction, measure the absorbance values of A1, A2 and A3 at 517 nm.

[0062] The calculation formula of scavenging rate is: Scavenging rate (%) = [1 - (A1 - A3) / A2] x 100%

[0063] The test results are shown in Table 4.

[0064] Table 4 Antioxidant test results

[0065]

[0066] According to Table 4, the differences after fermentation of different strains are huge, and even lower than the effect of commercially available products.

[0067] Example Four

[0068] Preparation of anti-aging composition, including the following steps:

[0069] According to the mass parts recorded in Table 5, weigh each raw material; mix uniformly and seal for storage.

[0070] Table 5 Anti-aging composition ratio

[0071]

[0072]

[0073] In Table 5, the fermented turmeric root extract is derived from the sample prepared in Example Two, No. 2; the fermented white Plumeria alba flower extract is derived from the sample prepared in Example One, No. 4.

[0074] Example Five

[0075] Performance test of anti-aging composition, including the following test contents:

[0076] Antioxidant performance test, the test method is the same as example three, the sample concentration is 0.5%; the results are shown in table 6 below.

[0077] Table 6 antioxidant test results

[0078]

[0079] According to the data in table 6, the five raw materials of tripeptide-1, palmitoyl pentapeptide-4, fermented turmeric root extract, fermented white plumeria flower extract and mullein extract can be ranked in descending order of their ability to scavenge free radicals as follows: tripeptide-1> fermented white plumeria flower extract> fermented turmeric root extract> mullein extract> palmitoyl pentapeptide-4.

[0080] Promote type I collagen test, the test method is as follows:

[0081] Elastic fibers are composed of elastin and microfibrils, which are distributed in the dermis and subcutaneous tissue, giving the skin elasticity. Due to the effects of ultraviolet radiation, stress, pollution and other environmental factors, the body's elastase production is promoted. Elastase is a member of the chymotrypsin family, which can degrade elastin, causing the loss of connective tissue in the epidermis, leading to skin aging, wrinkles and photoaging. In this experiment, porcine pancreatic elastase was used as the research object, and N-succinyl-alanine-alanine-alanine-p-nitroaniline (AAAPVN) was used as the substrate. Porcine pancreatic elastase can hydrolyze AAAPVN, and its hydrolysis product can cause an increase in absorbance at 420 nm. The absorbance was measured by an enzyme marker, which can evaluate the firming and anti-wrinkle efficacy of the sample to be tested.

[0082] 1. Solution preparation

[0083] (1) Sample concentration setting: select the anti-aging composition prepared in example four, and the test concentration is 0.5%.

[0084] (2) Preparation of Tris-HCl buffer solution (0.1M pH=8.0): weigh 2.42g of Tris in a beaker, add 200mL of ultrapure water, and dissolve completely. Adjust the pH to 8.0 with concentrated HCl.

[0085] (3) Preparation of positive control tea polyphenol solution (1mg / ml, 0.1%): weigh 5mg of tea polyphenol, and dissolve with 5mL of Tris-HCl buffer solution.

[0086] (4) Preparation of substrate solution AAAPVN (2mM): weigh 4.51mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline, and dissolve with 5ml of Tris-HCl buffer solution.

[0087] (5) Preparation of porcine pancreatic elastase solution (0.171 U / mL): 280 ul was dissolved in 10 ml Tris-HCl buffer.

[0088] 2. Sample addition

[0089] The experiment was divided into 4 groups, namely sample group, positive control group, blank control group and model control group. Four replicate wells were set for the same concentration in the same group. The amount of each solution added is shown in Table 7.

[0090] Table 7 Experimental grouping

[0091]

[0092] 3. Measurement

[0093] The reaction was placed at room temperature for 15 min, and the absorbance was measured at 420 nm using an enzyme marker.

[0094] 4. Result calculation

[0095]

[0096] In the formula: A0 is the average absorbance of the blank control well;

[0097] A1 is the average absorbance of the sample well;

[0098] A2 is the average absorbance of the model control well.

[0099] 5. Data statistics

[0100] SPSS22.0 statistical software package was used for statistical analysis, and the measurement values of the test area were subjected to descriptive statistics. The changes of numerical values were calculated and analyzed, and the differences between the control group and the sample group were compared. If the test data is normally distributed, independent T test method is used for statistical analysis; if the test data is not normally distributed, rank sum test method is used for statistical analysis. The statistical method uses double-tailed test, and the test level a = 0.05.

[0101] 6. Result determination

[0102] Compared with the model control group, the absorbance of the sample group showed a significant positive difference, indicating that the measured sample had an inhibitory effect on porcine pancreatic elastase. Otherwise, it has no effect.

[0103] 7. The experimental results are shown in Table 8.

[0104] Table 8 Porcine pancreatic elastase inhibition rate test results

[0105]

[0106] The samples in Table 8 were derived from the anti-aging composition prepared in Example Four.

[0107] Compared with the model control group, the positive control tea polyphenol elastase inhibition rate was 68.43%, and the absorbance was significantly different from the model group, proving that the experiment was effective.

[0108] Compared with the model control group, the absorbance of samples 1-10 was significantly different from the model control group (p<0.05). It is proved that the aforementioned composition has inhibitory effect on porcine pancreatic elastase and has certain firming and anti-wrinkle effect.

[0109] Example six

[0110] The preparation of the anti-aging freeze-dried powder comprises the following steps:

[0111] According to the mass parts recorded in Table 9, each raw material is weighed; after being mixed uniformly, it is sent to a freeze dryer for freeze-drying treatment; and then it is sealed and stored.

[0112] The samples of each serial number in Table 9 are additionally added with a freeze-drying protective agent. For example, when vitamin E is added, trehalose is added; when aidi benzene is added, mannitol and collagen are added; such freeze-drying protective agents are not shown again in Table 9.

[0113] The fermented turmeric root extract and the fermented white plumeria extract in Table 9 are pre-frozen, and do not need to be frozen again, but are mixed uniformly after the other ingredients are freeze-dried.

[0114] Table 9 Anti-aging freeze-dried powder ratio

[0115] Raw Material (mass parts) / No. 1 2 3 Tripeptide-1 0.1 0.1 0.1 Palmitoyl pentapeptide-4 0.04 0.04 0.04 Fermented curcuma longa root extract 4.00 4.00 4.00 Fermented plumeria alba flower extract 2.0 2.0 2.0 Alchemilla vulgaris extract 1.0 1.0 1.0 Vitamin E 0.01 0.00 0.00 Butylated hydroxytoluene 0.00 0.30 0.00 Edifenprox 0.00 0.00 0.50

[0116] Example seven

[0117] The anti-aging freeze-dried powder is tested for antioxidant performance, and the test method is the same as that in Example three, and the sample concentration is 0.1%, 0.5%, and 2.5%. The results are shown in Table 10.

[0118] Table 10 Antioxidant test results

[0119]

[0120] According to the experimental results in Table 10, after being prepared into a freeze-dried powder, the additional antioxidant and the type of antioxidant basically do not affect the performance.

[0121] The above detailed description is a specific description of one of the feasible embodiments of the present application, and this embodiment is not used to limit the patent scope of the present application. Any equivalent implementation or change that does not deviate from the present application should be included in the scope of the technical solutions of the present application.

Claims

1. An anti-aging plant essence, characterized in that, The anti-aging plant essence comprises the following ingredients by mass fraction: Tripeptide-1 0.1-0.5 Palmitoyl pentapeptide-4 0.04-0.1 Curcuma longa L. root extract 4-8 Plumeria alba L. flower extract 2-4 Alchemilla vulgaris L. extract 1-2 The curcuma longa L. root extract is a product obtained by purifying the product after anaerobic fermentation of curcuma longa L. by Lactobacillus rhamnosus; The anaerobic fermentation is carried out at 35-38℃ and pH 6.0-7.0 for at least 72 h; The Plumeria alba L. flower extract is a product obtained by purifying the product after aerobic solid-state fermentation of Plumeria alba L. by Aspergillus oryzae; In the preparation method of the Plumeria alba L. flower extract, the aerobic solid-state fermentation is carried out at 28-32℃ and pH 6.5-6.8 for at least 60 h.

2. The anti-aging plant essence according to claim 1, characterized in that, The preparation method of the curcuma longa L. root extract comprises the following steps: mixing activated Lactobacillus rhamnosus and curcuma longa L. fermentation substrate, then carrying out anaerobic fermentation, centrifuging, concentrating and purifying, and drying; The curcuma longa L. fermentation substrate comprises curcuma longa L., glucose, bran, low oligoisomaltose, dipotassium hydrogen phosphate and water.

3. The anti-aging plant essence according to claim 1, characterized in that, The preparation method of the Plumeria alba L. flower extract comprises the following steps: mixing activated Aspergillus oryzae and Plumeria alba L. flower fermentation substrate, then carrying out aerobic solid-state fermentation, centrifuging, concentrating and purifying, and drying; The Plumeria alba L. flower fermentation substrate comprises Plumeria alba L. flower, bran, corn starch, dipotassium hydrogen phosphate, lactose and water.

4. The anti-aging plant essence according to claim 1, characterized in that, The anti-aging plant essence further comprises an antioxidant; the antioxidant is at least one of vitamin E, butylated hydroxytoluene and edifenphos; the antioxidant is added in an amount of 0.01-0.

5.

5. The anti-aging plant essence according to claim 1, characterized in that, The dosage form of the anti-aging plant essence is freeze-dried powder.

6. The anti-aging plant essence according to claim 5, characterized in that, When the anti-aging plant essence contains an antioxidant, the freeze-drying protective agent is further included.

7. Use of the anti-aging plant essence according to any one of claims 1-6 in the preparation of a cosmetic.

8. Use according to claim 7 for the preparation of a cosmetic product, characterized in that, The minimum addition amount is 0.1 wt%.

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