Ginseng moxibustion stick with anti-fatigue effect and preparation method thereof

Abstract

The invention relates to the technical field of moxibustion stick preparation, and discloses a ginseng moxibustion stick with an anti-fatigue effect and a preparation method thereof.The preparation method comprises the following steps that moxa is sieved through a 80-mesh sieve to remove coarse particles, then the moxa, ginseng extract, schisandrin B, modified chitosan, modified silk fibroin and glycerin are put into a three-dimensional mixing machine to be stirred and mixed for 30-40 min, and a mixture is obtained; and finally, adding musk, continuously stirring for 15-20 minutes, standing and aging for 1-2 hours, keeping the pressure for 5-10 seconds under the pressure of 80-100 MPa by using an automatic forming machine, pressing into a moxibustion stick with the diameter of 1.8 cm and the length of 20 cm, controlling the density to be 0.8-1.0 g / cm < 3 >, circularly drying for 4-5 hours under hot air at the temperature of 35-45 DEG C, balancing for 45-50 hours in an environment with the humidity of 50%, and performing vacuum drying to obtain the ginseng moxibustion stick with the anti-fatigue effect. The moxibustion stick disclosed by the invention has an anti-fatigue effect and a good antibacterial property.

Description

Technical Field

[0001] The present application relates to the technical field of moxibustion stick preparation, and more specifically, to a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof. Background Art

[0002] Moxibustion, a key component of traditional Chinese medicine, uses the warmth generated by burning moxa or other media to stimulate acupuncture points, warming the meridians, harmonizing qi and blood, and strengthening the body's defenses and dispelling pathogenic factors. Existing moxibustion sticks offer advantages such as ease of use and definite efficacy. However, their limited functionality, relying primarily on a warming effect, has increasingly proven insufficient in alleviating fatigue and regulating sub-health conditions. Therefore, addressing this issue is crucial.

[0003] For example, patent CN118873482A discloses an aromatic moxibustion stick and its preparation method. The stick is made from the following ingredients: 200 parts of mugwort, 5-10 parts of agarwood, 5-10 parts of costus root, 2-5 parts of myrrh, 5-10 parts of frankincense, 5-10 parts of spikenard, 5-10 parts of cassia twig, 2-5 parts of mint, 2-5 parts of fennel, 2-5 parts of angelica, 2-5 parts of liquorice, 2-5 parts of dalbergia odorifera, and 2-5 parts of salvia miltiorrhiza. The smoke produced by the aromatic moxibustion stick produced by this invention has an aromatic odor upon combustion, is non-pungent, and can effectively reduce the content of harmful gases and tar in the smoke. It is green, safe, and environmentally friendly, but lacks anti-fatigue effects and its antibacterial properties need to be improved.

[0004] Based on the above statements, the present invention provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof. Summary of the Invention

[0005] In order to prepare antibacterial and anti-fatigue ginseng moxibustion sticks, the present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof.

[0006] The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical scheme: a ginseng moxibustion stick with anti-fatigue effect, comprising the following components by weight: 45-50 parts by weight of moxa, 8-12 parts by weight of ginseng extract, 5-10 parts by weight of schisandra chinensis B, 3-5 parts by weight of modified chitosan, 2-4 parts by weight of modified silk fibroin, 0.2-0.3 parts by weight of musk, and 1-3 parts by weight of glycerin; Among them, the modified chitosan is obtained by modifying chitosan with epoxy quaternary ammonium salt; Modified silk fibroin eggs are obtained by modifying thiolated silk fibroin with methacryloyloxyethyltrimethylammonium chloride and tea polyphenols.

[0007] Furthermore, the preparation method of the modified chitosan is: swelling chitosan in acetic acid solution for 1-2 hours, then adding 2,3-epoxypropyltrimethylammonium chloride thereto, stirring and mixing, then adding dilute hydrochloric acid, reacting for 10-12 hours, after the reaction is completed, filtering, washing, and drying to obtain modified chitosan.

[0008] During the above reaction, chitosan is swollen in a weak acid environment, so that the amino groups on the chitosan molecular weight are protonated -NH3 + The swelling causes the chitosan molecular chain to stretch, forming a viscous solution and exposing more reaction sites. It is then mixed with epoxy quaternary ammonium salt. The protonated amino group of chitosan acts as a nucleophile to attack the epoxy group, resulting in ring opening and the formation of CN covalent bonds, connecting the quaternary ammonium group to the chitosan main chain to prepare modified chitosan.

[0009] Furthermore, the usage ratio of the chitosan, acetic acid solution, 2,3-epoxypropyltrimethylammonium chloride, and dilute hydrochloric acid is 2.12-2.16 g: 22-25 mL: 2.03-2.08 g: 0.4-0.5 mL.

[0010] Furthermore, the reaction temperature is 70-80°C.

[0011] Furthermore, the preparation method of the modified silk fibroin is: Step 1: Swelling the thiolated silk fibroin in deionized water at 0-4°C for 1-2 hours, dialyzing for 50-70 hours, then adding methacryloyloxyethyltrimethylammonium chloride and ammonium persulfate, stirring and mixing, reacting at 30-50°C for 18-22 hours, and adjusting the pH to 7.0-8.0 after the reaction, dialyzing, centrifuging, and drying to obtain intermediate 1; Step 2: Add intermediate 1 and tea polyphenols to the reactor, stir and mix, add p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 45-60°C for 16-24 hours. After the reaction is completed, cool, dialyze, concentrate, and dry to obtain modified silk fibroin.

[0012] During the above reaction process, the thiol-modified silk fibroin is fully swollen in water at low temperature to expose the active thiol and hydroxyl groups in the molecular chain, providing active sites for subsequent reactions. The methacryloyloxyethyltrimethylammonium chloride free radical reacts with the thiol and hydroxyl groups of the thiol-modified silk fibroin to form a covalent bond through thiol-ene click chemistry or a free radical transfer mechanism to generate a graft copolymer. Subsequently, under the catalysis of p-toluenesulfonic acid, the phenolic hydroxyl groups of the tea polyphenols can undergo a condensation reaction with the amino groups of the intermediate 1 to form a phenolamine bond to prepare the modified silk fibroin.

[0013] Furthermore, in the step 1, the usage ratio of thiolated silk fibroin, deionized water, methacryloyloxyethyltrimethylammonium chloride, and ammonium persulfate is 3.12-3.16 g: 28-32 mL: 2.53-2.58 g: 0.1-0.12 g.

[0014] Furthermore, the usage ratio of the intermediate 1, tea polyphenols, and p-toluenesulfonic acid in the step 2 is 3.61-3.65 g: 4.12-4.17 g: 0.08-0.09 g.

[0015] Furthermore, the dialysis time in step 1 is 36-48 hours, and the dialysis time in step 2 is 12-24 hours.

[0016] Furthermore, during the molding process, the pressure was set to 80-100 MPa, the pressure was maintained for 5-10 seconds, and the density was controlled to 0.8-1.0 g / cm 3 .

[0017] The preparation method of the ginseng moxibustion stick with anti-fatigue effect specifically comprises the following steps: The moxa is sieved to remove coarse particles, and then placed in a three-dimensional mixer with ginseng extract, schisandra chinensis B, modified chitosan, modified silk fibroin, and glycerol and stirred for 30-40 minutes. Finally, musk is added and stirring is continued for 15-20 minutes. After standing and aging for 1-2 hours, it is formed using an automatic molding machine, and then circulated and dried under hot air at 35-45°C for 4-5 hours, balanced for 45-50 hours, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effects.

[0018] Furthermore, during the molding process, the pressure was set to 80-100 MPa, the pressure was maintained for 5-10 seconds, and the density was controlled to 0.8-1.0 g / cm 3 .

[0019] Beneficial effects of the present invention: 1. Ginsenosides in ginseng extract promote glucose uptake and fatty acid oxidation, increasing ATP production. Schisandrin B protects mitochondrial membrane integrity, complementing the ATP-generating effects of ginsenosides and contributing to fatigue-fatigue relief. Chitosan's film-forming and adsorbent properties encapsulate the active ingredients in ginseng, allowing them to be slowly released during moxibustion, prolonging their effectiveness.

[0020] 2. The free thiol groups in thiolated silk fibroin can scavenge the reactive oxygen species produced during aerobic metabolism and reduce muscle fatigue caused by oxidative stress. By modifying chitosan and silk fibroin and introducing quaternary ammonium groups, the electrostatic binding ability with negatively charged ginsenosides and schisandra chinensis B molecules can be improved, thereby enhancing drug loading efficiency. In addition, the quaternary ammonium groups can give the material broad-spectrum antibacterial properties, thereby improving the antibacterial properties of moxibustion sticks and reducing the risk of skin infection during moxibustion therapy. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 This is the H-NMR spectrum of the modified chitosan in Example 1 of the present invention.

[0023] Figure 2 This is the H NMR spectrum of the intermediate 1 in Example 1 of the present invention. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0025] In the following specific embodiments, the raw materials used are chemically pure. In addition: The chitosan used has a deacetylation degree of 95%.

[0026] The preparation method of the ginseng extract used is: 100g of dried ginseng medicinal material was processed into coarse powder with a pulverizer. According to a ratio of 1:10, 1000mL of 70% ethanol solution was added to the coarse powder and soaked for 3 hours. Afterwards, it was heated in a water bath under reflux for 1.5 hours and filtered to obtain the first extract. 1000mL of 70% ethanol solution was added again, and the water bath under reflux was repeated for 1 hour, and the extract was filtered again to obtain the second extract. After the extracts obtained by the two filtrations were combined, rotary evaporation was performed and concentrated to 50mL. Subsequently, the rotary evaporation product was placed in a vacuum drying oven for drying to obtain ginseng extract.

[0027] The preparation method of the used Schisandrin B is: 500 g of dried, mature Schisandra chinensis fruit was ground into a coarse powder (40-mesh sieve) and then soaked in 95% ethanol. 5000 mL of ethanol was added at a ratio of 1:10. The extract was soaked at room temperature for 24 hours, stirring every 6 hours. The extract was filtered through filter paper and concentrated to a paste using a rotary evaporator at 40°C under reduced pressure to remove the ethanol. The extract was first extracted three times with an equal volume of n-hexane to remove fat-soluble impurities. The aqueous phase was then extracted three times with ethyl acetate at a ratio of 1:1. The ethyl acetate layer was then removed by rotary evaporation to obtain a crude extract containing schisandrin B. This crude extract was then purified by 60-100 mesh silica gel column chromatography using a n-hexane / ethyl acetate gradient elution. Fractions containing schisandrin B were analyzed by TLC and pooled. The sample was dissolved in acetonitrile and separated using a C18 reverse-phase column with a mobile phase of acetonitrile and water (70:30) and a detection wavelength of 254 nm. The schisandrin B fraction with a purity of ≥98% by high-performance liquid chromatography was collected. The acetonitrile solvent was removed by rotary evaporation, and the schisandrin B was finally dried in a vacuum desiccator to a constant weight to obtain high-purity schisandrin B powder.

[0028] The preparation method of the thiolated silk fibroin used is: Weigh 10g of silkworm cocoons and boil them in 4L of 0.05mol / L sodium carbonate aqueous solution for 30 minutes. Remove and repeat the above steps twice. Rinse the cocoons with deionized water and dry them to obtain degummed silk. Immerse 5.2g of degummed silk in approximately 75mL of a solution of calcium chloride-ethanol-water (molar ratio of 1:2:8) and stir at 70°C for 4 hours to obtain a silk fibroin solution.

[0029] After cooling, the solution was centrifuged at room temperature for 6 minutes to remove insoluble impurities. The dissolved silk fibroin salt solution was placed in a dialysis bag with a molecular weight cutoff of 3500 and dialyzed against deionized water at 4°C for 3 days. The pH of the dialyzed silk fibroin solution was adjusted with a 0.1 mol / L aqueous solution of 2-morpholinoethanesulfonic acid (containing 0.05 mol / L sodium chloride) at a pH of 6. Once the pH stabilized at 6, 0.04 L of 0.3 mol / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 0.06 L of 0.5 mol / L N-hydroxysuccinimide were added for activation at room temperature for 30 minutes. 0.09 L of 0.18 mol / L reduced glutathione was then added for 18 hours. The reaction solution was placed in a dialysis bag with a molecular weight cutoff of 3500 Da and dialyzed against deionized water at 4°C for 2 days. The solution was then freeze-dried to obtain the thiolated silk fibroin.

[0030] Example 1 The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical solution: (1) 2.12 g of chitosan was swelled in 22 mL of 3% acetic acid solution for 1 h, and then 2.03 g of 2,3-epoxypropyltrimethylammonium chloride was added thereto, stirred and mixed, and then 0.4 mL of 3% dilute hydrochloric acid was added. The mixture was reacted at 70 ° C for 10 h. After the reaction was completed, the mixture was filtered, washed with deionized water 3 times, and then washed with 70% ethanol once, and vacuum dried at 45 ° C to obtain modified chitosan. The reaction process is as follows:

[0031] (2) At 0°C, 3.12 g of thiolated silk fibroin was swollen in 28 mL of deionized water for 1 h and dialyzed for 50 h. Then, 2.53 g of methacryloyloxyethyl trimethylammonium chloride and 0.1 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 30°C for 18 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.0. A dialysis bag with a cutoff of 3.5 kDa was selected for dialysis for 36 h. The mixture was centrifuged at a speed of 8000 rpm for 15 min and dried at 45°C for 2 h to obtain intermediate 1. The reaction process is as follows:

[0032] (3) Add 3.61 g of intermediate 1 and 4.12 g of tea polyphenols to the reactor, stir and mix, add 0.08 g of p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 45 °C for 16 h. After the reaction is completed, cool to room temperature, select a dialysis bag with a cutoff of 3 kDa for 12 h, vacuum rotary evaporation and concentration, and dry at 50 °C for 3 h to obtain modified silk fibroin; (4) 45 parts by weight of moxa were passed through an 80-mesh sieve to remove coarse particles, and then placed in a three-dimensional mixer with 8 parts by weight of ginseng extract, 5 parts by weight of schisandra chinensis B, 3 parts by weight of modified chitosan, 2 parts by weight of modified silk fibroin, and 1 part by weight of glycerol, and stirred for 30 minutes. Finally, 0.2 parts by weight of musk were added and stirred for 15 minutes. After standing for 1 hour, the mixture was pressed into moxa sticks with a diameter of 1.8 cm and a length of 20 cm using an automatic molding machine at a pressure of 80 MPa for 5 seconds. The density was controlled to be 0.8 g / cm 3 , circulated and dried under 35℃ hot air for 4h, then placed in an environment with 50% humidity for 45h, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effect.

[0033] Example 2 The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical solution: (1) 2.16 g of chitosan was swelled in 25 mL of 3% acetic acid solution for 2 h, and then 2.08 g of 2,3-epoxypropyltrimethylammonium chloride was added thereto, stirred and mixed, and then 0.5 mL of 3% dilute hydrochloric acid was added. The mixture was reacted at 80 ° C for 12 h. After the reaction was completed, the mixture was filtered, washed with deionized water for 3 times, and then washed with 70% ethanol once, and vacuum dried at 40 ° C to obtain modified chitosan. (2) At 4°C, 3.16 g of thiolated silk fibroin was swollen in 32 mL of deionized water for 2 h and dialyzed for 70 h. Then, 2.58 g of methacryloyloxyethyl trimethylammonium chloride and 0.12 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 50°C for 22 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.0. The product was dialyzed for 36 h using a dialysis bag with a cutoff of 3.5 kDa. The product was centrifuged at a speed of 8000 rpm for 15 min and dried at 45°C for 2 h to obtain intermediate 1. (3) Add 3.65 g of intermediate 1 and 4.17 g of tea polyphenols to the reactor, stir and mix, add 0.09 g of p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 60 ° C for 24 h. After the reaction is completed, cool to room temperature, select a dialysis bag with a cutoff of 3 kDa for 12 h, and concentrate by vacuum rotary evaporation. Dry at 50 ° C for 3 h to obtain modified silk fibroin; (4) 50 parts by weight of moxa were passed through an 80-mesh sieve to remove coarse particles, and then placed in a three-dimensional mixer with 12 parts by weight of ginseng extract, 10 parts by weight of schisandra chinensis B, 5 parts by weight of modified chitosan, 4 parts by weight of modified silk fibroin, and 3 parts by weight of glycerol, and stirred for 40 minutes. Finally, 0.3 parts by weight of musk were added and stirred for 20 minutes. After standing and aging for 2 hours, an automatic molding machine was used to press the moxa sticks with a diameter of 1.8 cm and a length of 20 cm at a pressure of 100 MPa for 10 seconds. The density was controlled to be 1.0 g / cm 3 , circulated and dried under 45℃ hot air for 5h, then placed in an environment with 50% humidity for 50h, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effect.

[0034] Example 3 The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical solution: (1) 2.14 g of chitosan was swelled in 24 mL of 3% acetic acid solution for 1 h, and then 2.05 g of 2,3-epoxypropyltrimethylammonium chloride was added thereto, stirred and mixed, and then 0.4 mL of 3% dilute hydrochloric acid was added. The mixture was reacted at 75 °C for 11 h. After the reaction was completed, the mixture was filtered, washed twice with deionized water, and then washed twice with 70% ethanol, and dried in vacuum at 45 °C to obtain modified chitosan. (2) At 2°C, 3.14 g of thiolated silk fibroin was swollen in 30 mL of deionized water for 1 h and dialyzed for 60 h. Then, 2.55 g of methacryloyloxyethyltrimethylammonium chloride and 0.11 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 40°C for 20 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.5. The product was dialyzed for 36 h using a dialysis bag with a cutoff of 3.5 kDa. The product was centrifuged at a speed of 8000 rpm for 15 min and dried at 45°C for 2 h to obtain intermediate 1. (3) Add 3.63 g of intermediate 1 and 4.15 g of tea polyphenols to the reactor, stir and mix, add 0.08 g of p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 50 °C for 20 h. After the reaction is completed, cool to room temperature, select a dialysis bag with a cutoff of 3 kDa for 12 h, and concentrate by vacuum rotary evaporation. Dry at 50 °C for 3 h to obtain modified silk fibroin; (4) 48 parts by weight of moxa were passed through an 80-mesh sieve to remove coarse particles, and then placed in a three-dimensional mixer with 10 parts by weight of ginseng extract, 8 parts by weight of schisandra chinensis B, 4 parts by weight of modified chitosan, 3 parts by weight of modified silk fibroin, and 2 parts by weight of glycerol, and stirred for 35 minutes. Finally, 0.2 parts by weight of musk were added and stirred for 18 minutes. After standing for 1 hour, the mixture was pressed into moxa sticks with a diameter of 1.8 cm and a length of 20 cm using an automatic molding machine at a pressure of 90 MPa for 8 seconds. The density was controlled to be 0.9 g / cm 3 , dried in a hot air cycle at 40°C for 4 hours, then placed in an environment with a humidity of 50% for 48 hours, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effects.

[0035] Example 4 The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical solution: (1) 2.13 g of chitosan was swelled in 23 mL of 3% acetic acid solution for 1 h, and then 2.04 g of 2,3-epoxypropyltrimethylammonium chloride was added thereto, stirred and mixed, and then 0.4 mL of 3% dilute hydrochloric acid was added. The mixture was reacted at 72 °C for 11 h. After the reaction was completed, the mixture was filtered, washed three times with deionized water, and then washed twice with 70% ethanol, and dried in vacuum at 40 °C to obtain modified chitosan. (2) At 1°C, 3.13 g of thiolated silk fibroin was swollen in 29 mL of deionized water for 1 h and dialyzed for 55 h. Then, 2.54 g of methacryloyloxyethyltrimethylammonium chloride and 0.1 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 35°C for 19 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.2. The product was dialyzed for 36 h using a dialysis bag with a cutoff of 3.5 kDa. The product was centrifuged at a speed of 8000 rpm for 15 min and dried at 45°C for 2 h to obtain intermediate 1. (3) Add 3.62 g of intermediate 1 and 4.14 g of tea polyphenols to the reactor, stir and mix, add 0.08 g of p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 48 °C for 18 h. After the reaction is completed, cool to room temperature, select a dialysis bag with a cutoff of 3 kDa for 12 h, and concentrate by vacuum rotary evaporation. Dry at 50 °C for 3 h to obtain modified silk fibroin; (4) 46 parts by weight of moxa were passed through an 80-mesh sieve to remove coarse particles, and then placed in a three-dimensional mixer with 9 parts by weight of ginseng extract, 6 parts by weight of schisandra chinensis B, 3 parts by weight of modified chitosan, 2 parts by weight of modified silk fibroin, and 2 parts by weight of glycerol, and stirred for 30 minutes. Finally, 0.2 parts by weight of musk were added and stirred for 16 minutes. After standing and aging for 1 hour, an automatic molding machine was used to press the moxa into moxibustion sticks with a diameter of 1.8 cm and a length of 20 cm at a pressure of 85 MPa for 6 seconds. The density was controlled to be 0.8 g / cm 3 , circulated and dried under 35℃ hot air for 4h, then placed in an environment with 50% humidity for 46h, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effect.

[0036] Example 5 The present application provides a ginseng moxibustion stick with anti-fatigue effect and a preparation method thereof, which adopts the following technical solution: (1) 2.15 g of chitosan was swelled in 24 mL of 3% acetic acid solution for 2 h, and then 2.06 g of 2,3-epoxypropyltrimethylammonium chloride was added thereto, stirred and mixed, and then 0.5 mL of 3% dilute hydrochloric acid was added. The mixture was reacted at 78 °C for 12 h. After the reaction was completed, the mixture was filtered, washed three times with deionized water, and then washed twice with 70% ethanol, and dried in vacuum at 50 °C to obtain modified chitosan. (2) At 3°C, 3.15 g of thiolated silk fibroin was swollen in 31 mL of deionized water for 2 h and dialyzed for 65 h. Then, 2.56 g of methacryloyloxyethyltrimethylammonium chloride and 0.12 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 45°C for 21 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.0. The product was dialyzed for 36 h using a dialysis bag with a cutoff of 3.5 kDa. The product was centrifuged at a speed of 8000 rpm for 15 min and dried at 45°C for 2 h to obtain intermediate 1. (3) Add 3.64 g of intermediate 1 and 4.16 g of tea polyphenols to the reactor, stir and mix, add 0.09 g of p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 55 °C for 22 h. After the reaction is completed, cool to room temperature, select a dialysis bag with a cutoff of 3 kDa for 12 h, and concentrate by vacuum rotary evaporation. Dry at 50 °C for 3 h to obtain modified silk fibroin; (4) 48 parts by weight of moxa were passed through an 80-mesh sieve to remove coarse particles, and then placed in a three-dimensional mixer with 11 parts by weight of ginseng extract, 9 parts by weight of schisandra chinensis B, 4 parts by weight of modified chitosan, 4 parts by weight of modified silk fibroin, and 3 parts by weight of glycerol, and stirred for 40 minutes. Finally, 0.3 parts by weight of musk were added and stirred for 18 minutes. After standing and aging for 2 hours, an automatic molding machine was used to press the mixture into moxibustion sticks with a diameter of 1.8 cm and a length of 20 cm at a pressure of 95 MPa for 10 seconds. The density was controlled to be 1.0 g / cm 3 , circulated and dried under 45℃ hot air for 5h, then placed in an environment with 50% humidity for 50h, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effect.

[0037] Comparative Example 1 Compared with Example 5, this comparative example differs in that chitosan is used instead of modified chitosan.

[0038] Comparative Example 2 Compared with Example 5, this comparative example differs in that thiolated silk fibroin is used instead of modified silk fibroin.

[0039] Comparative Example 3 Compared with Example 5, this comparative example is different in that silk fibroin is used instead of thiol-modified silk fibroin to prepare the modified silk fibroin. Specifically, step (2) in this comparative example is as follows: At 3°C, 3.15 g of silk fibroin was swollen in 31 mL of deionized water for 2 h and dialyzed for 65 h. Then, 2.56 g of methacryloyloxyethyltrimethylammonium chloride and 0.12 g of ammonium persulfate were added thereto, stirred and mixed, and reacted at 45°C for 21 h. After the reaction, sodium bicarbonate was added to adjust the pH to 7.0. A dialysis bag with a cutoff of 3.5 kDa was selected for dialyzation for 36 h, centrifuged at a speed of 8000 rpm for 15 min, and dried at 45°C for 2 h to obtain intermediate 1.

[0040] Comparative Example 4 Compared with Example 5, this comparative example differs in that schisandrin B is used instead of ginseng extract.

[0041] Performance Testing The moxibustion sticks prepared in Examples 1-5 and Comparative Examples 1-3 were subjected to performance tests.

[0042] (1) The anti-fatigue performance test method is as follows: After 3 days of adaptive training, healthy male mice were randomly divided into the Example group and the Comparative Example group, with 10 mice in each group. Each group of mice was fixed in a special fumigation cage and fumigated with the moxibustion sticks prepared in Examples 1-5 and Comparative Examples 1-3 at a set distance for 10 minutes per day for 7 days. Then, a lead sheet weighing 5% of the body weight was tied to the tail of each mouse. The mice were placed in a bucket of water at a temperature of (23±1)°C and a depth of 30 cm for a swimming test. The time from the start of swimming to exhaustion (the mouse sank underwater for 10 seconds and still could not reach the surface) was calculated as the mouse's weighted swimming time. The results are shown in Table 1.

[0043] Table 1: Fatigue resistance test table

[0044] As can be seen from Table 1, the moxibustion sticks prepared in Examples 1-5 can allow mice to swim longer, which is significantly higher than other comparative examples, indicating that the moxibustion sticks of the present invention have good anti-fatigue effects. As can be seen from the results in Comparative Example 1, chitosan modified with quaternary ammonium salts showed gains in both sustained-release efficacy and increased anti-fatigue effects. As can be seen from the results in Comparative Examples 2 and 3, silk fibroin can enhance drug loading efficiency and antioxidant capacity after sulfhydrylation and quaternary ammonium salt modification. The results in Comparative Example 4 verified that the combination of ginseng extract and schisandrin can promote glucose uptake and fatty acid oxidation, increase ATP generation, and schisandrin B can protect mitochondrial membrane integrity, complementing the ATP generation effect of ginsenosides, and the two have a synergistic anti-fatigue effect.

[0045] (2) The antibacterial performance test method is as follows: the smoke from the burning moxibustion stick is introduced into a closed chamber containing Staphylococcus aureus and Staphylococcus epidermidis bacterial liquid, and the survival rate of the bacterial liquid is measured after different 10-minute intervals. The results are shown in Table 2.

[0046] Table 2: Antibacterial performance test table

[0047] It can be seen from Table 2 that the moxibustion sticks prepared in Examples 1-5 resulted in a lower survival rate of the bacterial solution, indicating that the moxibustion sticks of the present invention have a good antibacterial effect.

[0048] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, method, article, or apparatus comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus comprising the element.

[0049] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

[0050] Those skilled in the art will appreciate that the foregoing descriptions are merely specific embodiments of the present invention, and not exhaustive. It should be noted that numerous variations and modifications are possible for those skilled in the art, and all such variations and modifications that do not exceed the scope of the claims should be considered within the scope of protection of the present invention.

Claims

1. A ginseng moxibustion stick with anti-fatigue effect, characterized in that: The invention comprises the following components by weight: 45-50 parts by weight of moxa, 8-12 parts by weight of ginseng extract, 5-10 parts by weight of schisandra chinensis B, 3-5 parts by weight of modified chitosan, 2-4 parts by weight of modified silk fibroin, 0.2-0.3 parts by weight of musk, and 1-3 parts by weight of glycerin; Among them, the modified chitosan is obtained by modifying chitosan with epoxy quaternary ammonium salt; The modified silk fibroin is obtained by modifying the thiolated silk fibroin with methacryloyloxyethyltrimethylammonium chloride and tea polyphenols.

2. The ginseng moxibustion stick with anti-fatigue effect according to claim 1, characterized in that: The preparation method of the modified chitosan comprises: swelling chitosan in an acetic acid solution for 1-2 hours, then adding 2,3-epoxypropyltrimethylammonium chloride thereto, stirring and mixing, then adding dilute hydrochloric acid, reacting for 10-12 hours, and after the reaction is completed, filtering, washing, and drying to obtain the modified chitosan.

3. The ginseng moxibustion stick with anti-fatigue effect according to claim 2, characterized in that: The usage ratio of the chitosan, acetic acid solution, 2,3-epoxypropyltrimethylammonium chloride and dilute hydrochloric acid is 2.12-2.16 g: 22-25 mL: 2.03-2.08 g: 0.4-0.5 mL.

4. The ginseng moxibustion stick with anti-fatigue effect according to claim 2, characterized in that: The reaction temperature is 70-80°C.

5. The ginseng moxibustion stick with anti-fatigue effect according to claim 1, characterized in that: The preparation method of the modified silk fibroin is as follows: Step 1: Swelling the thiolated silk fibroin in deionized water at 0-4°C for 1-2 hours, dialyzing for 50-70 hours, then adding methacryloyloxyethyltrimethylammonium chloride and ammonium persulfate, stirring and mixing, reacting at 30-50°C for 18-22 hours, and adjusting the pH to 7.0-8.0 after the reaction, dialyzing, centrifuging, and drying to obtain intermediate 1; Step 2: Add intermediate 1 and tea polyphenols to the reactor, stir and mix, add p-toluenesulfonic acid, and then add phosphate buffer to adjust the pH to neutral. React at 45-60°C for 16-24 hours. After the reaction is completed, cool, dialyze, concentrate, and dry to obtain modified silk fibroin.

6. The ginseng moxibustion stick with anti-fatigue effect according to claim 5, characterized in that: The usage ratio of thiolated silk fibroin, deionized water, methacryloyloxyethyltrimethylammonium chloride, and ammonium persulfate in the step 1 is 3.12-3.16 g: 28-32 mL: 2.53-2.58 g: 0.1-0.12 g.

7. The ginseng moxibustion stick with anti-fatigue effect according to claim 5, characterized in that: The usage ratio of the intermediate 1, tea polyphenols, and p-toluenesulfonic acid in the step 2 is 3.61-3.65 g: 4.12-4.17 g: 0.08-0.09 g.

8. The ginseng moxibustion stick with anti-fatigue effect according to claim 5, characterized in that: The dialysis time in step 1 is 36-48 hours, and the dialysis time in step 2 is 12-24 hours.

9. A method for preparing a ginseng moxibustion stick with anti-fatigue effect as claimed in any one of claims 1 to 8, characterized in that: The specific steps include: The moxa is sieved to remove coarse particles, and then placed in a three-dimensional mixer with ginseng extract, schisandra chinensis B, modified chitosan, modified silk fibroin, and glycerol and stirred for 30-40 minutes. Finally, musk is added and stirring is continued for 15-20 minutes. After standing and aging for 1-2 hours, it is formed using an automatic molding machine, and then circulated and dried under hot air at 35-45°C for 4-5 hours, balanced for 45-50 hours, and vacuum dried to obtain ginseng moxibustion strips with anti-fatigue effects.

10. The method for preparing the ginseng moxibustion stick with anti-fatigue effect according to claim 9, characterized in that: During the molding process, the pressure is set to 80-100 MPa, the pressure is maintained for 5-10 seconds, and the density is controlled at 0.8-1.0 g / cm 3 .

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