Multifunctional hydrogel as well as preparation method and application thereof

Abstract

The invention relates to the technical field of material preparation, in particular to multifunctional hydrogel and a preparation method and application thereof.The preparation method comprises the following steps that S1, an acrylamide-hydrolyzed elastin-mixed solution is prepared, specifically, a preparation device is prepared, 2.6 g of an acrylamide monomer and hydrolyzed elastin with different mass fractions are weighed and dissolved in deionized water, and the acrylamide-hydrolyzed elastin-mixed solution is prepared; stirring the acrylamide and the hydrolyzed elastin at the room temperature of 22-26 DEG C for 25-30 minutes by using a preparation device, and dissolving the acrylamide and the hydrolyzed elastin to obtain a mixed solution of the acrylamide and the hydrolyzed elastin; s2, adding an initiator and a cross-linking agent: sequentially adding the initiator APS and the cross-linking agent MBA into the mixed solution prepared in the step S1, stirring for 15 minutes by using a preparation device at the room temperature of 22-26 DEG C, and mixing and dissolving the cross-linking agent MBA, the initiator APS and the mixed solution; and S3, preparing the callus hydrogel. The method is complete in logic, and the preparation efficiency of the hydrogel can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of material preparation, and in particular to a multifunctional hydrogel and a preparation method and application thereof. Background Art

[0002] Hydrogels are a class of materials with three-dimensional network structures formed through chemical (covalent) or physical (non-covalent) crosslinking. Their soft texture and high water content make them widely used in the biomedical field. Due to their excellent bio-tissue compatibility, controllable mechanical properties, and rich functional groups, hydrogels have gradually become a research hotspot for medical wound dressings.

[0003] The preparation process of hydrogel is relatively complicated, requiring the continuous addition of different preparation materials, multiple mixing and shaking, and then filtering to prepare a small amount of hydrogel. In existing hydrogel preparation technology, when mixing, shaking and filtering the solution, multiple equipment such as mixing equipment, shaking equipment and filtering equipment are required to prepare the hydrogel, resulting in high cost of hydrogel preparation.

[0004] In summary, how to solve the existing hydrogel preparation technology,

[0005] The high cost of preparing hydrogels, which requires multiple devices such as mixing, shaking, and filtering to mix, shake, and filter solutions, has become a pressing challenge in the field. Therefore, it is necessary to develop a more rational multifunctional hydrogel, its preparation method, and its application. Summary of the Invention

[0006] To solve the above problems, the present invention provides a multifunctional hydrogel, a preparation method and application thereof. By improving the hydrogel preparation method, the time required for preparation can be effectively shortened, the complexity of hydrogel preparation can be reduced, and the preparation efficiency of hydrogel can be improved. By improving the preparation device, the preparation cost of hydrogel can be effectively reduced.

[0007] In order to achieve the above-mentioned purpose, the technical solution of the present invention is as follows: a multifunctional hydrogel, including a callus hydrogel, which comprises the following components: 10-20 parts of acrylamide monomer, 1-5 parts of hydrolyzed elastin, 1-2 parts of crosslinking agent MBA and 1-2 parts of initiator APS.

[0008] Furthermore, a method for preparing a multifunctional hydrogel comprises the following steps:

[0009] S1. Preparation of acrylamide-hydrolyzed elastin mixed solution:

[0010] Prepare a preparation device, weigh 2.6 g of acrylamide monomer and different mass fractions of hydrolyzed elastin, and dissolve them in deionized water; stir them using the preparation device at room temperature of 22-26°C for 25-30 minutes to dissolve the acrylamide and hydrolyzed elastin, thereby obtaining a mixed solution of acrylamide and hydrolyzed elastin.

[0011] S2, addition of initiator and crosslinker:

[0012] The initiator APS was added to the mixed solution prepared in S1, and stirred for 15 minutes using a preparation device at room temperature of 22-26°C to mix and dissolve the initiator APS and the mixed solution; then the cross-linker MBA was added to the mixed solution, and stirred for another 15 minutes using a preparation device at room temperature of 22-26°C; finally, the dissolved mixed solution was shaken for 5-10 minutes using a preparation device.

[0013] S3, Preparation of Callus Hydrogel:

[0014] At room temperature of 22-26°C, catalyst TMEDA was added to the mixed solution prepared in S2, and the mixture was stirred for 15 minutes using a preparation device; the mixed solution was poured into a polytetrafluoroethylene mold, and the mixture was allowed to react at room temperature of 22-26°C for 90-120 minutes to obtain a callus hydrogel; the callus hydrogel was rinsed with deionized water for 30-35 minutes.

[0015] Furthermore, in S1, the mass fraction of acrylamide is 26%, and the mass fractions of hydrolyzed elastin include 0.5%, 1.0%, 2.0% and 3.0%.

[0016] Furthermore, in S2, the power of the ultrasonic equipment is controlled at 200-300w, and the ultrasonic treatment is performed for 25-30min.

[0017] The above scheme has the following beneficial effects:

[0018] 1. Existing hydrogel preparation formulas are single, and the hydrogels prepared are deficient in elasticity, tensile strength, and biocompatibility. Pure AM hydrogels form a network through chemical cross-linking, but they are brittle when the cross-linking density is high, and insufficient in strength when the cross-linking density is low. They have low tensile strength and limited elongation at break (<200%), making it difficult to meet the flexibility and dynamic deformation capabilities required for skin adhesion. Residual AM monomers are neurotoxic and may cause inflammation; the hydrophobic surface leads to poor cell adhesion and low cell affinity, which is not conducive to wound healing. This method optimizes the preparation formula of existing hydrogels. Unlike the existing technology, this method combines acrylamide with hydrolyzed elastin in different mass ratios, not only maintaining the basic physical and chemical properties of the hydrogel, but also introducing the biocompatibility and bioactivity of elastin, allowing the hydrogel to better adhere to the user's skin and have better skin affinity. The addition of hydrolyzed elastin may enhance the elasticity, flexibility, and cell affinity of the hydrogel, allowing the hydrogel prepared by this method to better play a role in wound healing. Hydrolyzed elastin contains hydrophobic domains and hydrophilic cross-linked regions, exposing more active sites upon hydrolysis. When copolymerized with AM, the hydrophobic regions form multiscale networks through physical entanglement or chemical cross-linking. The RGD sequence in hydrolyzed elastin promotes integrin-mediated cell adhesion and increases fibroblast proliferation.

[0019] 2. This method promotes effective crosslinking between monomers by adding a crosslinker, MBA, and an initiator, APS, during the preparation process, followed by stirring and ultrasonic treatment under appropriate conditions. This results in a stable three-dimensional network structure, which in turn helps enhance the mechanical strength and durability of the hydrogel. MBA (N,N'-methylenebisacrylamide) serves as a crosslinker. The acrylamide groups (–CONH2) at both ends of its molecule react with amino groups (–NH2) in acrylamide (AM) monomers or elastin molecules during free radical polymerization initiated by APS (ammonium persulfate), forming irreversible covalent crosslinks. Elastin chains contain hydrophobic domains and hydrophilic segments, which form reversible physical crosslinks through hydrophobic interactions and hydrogen bonding.

[0020] 3. The present invention can significantly improve the preparation efficiency through the design of the preparation device; the preparation device can simultaneously complete multiple steps such as stirring, mixing, shaking, filtering and temperature adjustment of multiple raw materials, is simple to operate, and can effectively improve the preparation efficiency of hydrogels; and multiple functions are integrated into a set of equipment, thereby effectively reducing the preparation cost of hydrogels.

[0021] Furthermore, in S1, the preparation device includes a controller and a housing, and the housing is provided with a stirring component for stirring the mixed solution, a filtering component for filtering the mixed solution, and a shaking component for shaking the mixed solution.

[0022] The stirring assembly includes a first bracket fixedly connected to the outer shell and a stirring box, and a double-headed driving member is embedded in the first bracket; a main bevel gear is coaxially fixedly connected to one of the output shafts of the double-headed driving member, and the main bevel gear is engaged with a secondary bevel gear, and a rotating shaft is coaxially fixedly connected to the bottom of the secondary bevel gear, and a number of stirring rods are fixedly connected to the rotating shaft; the bottom of the rotating shaft extends to the interior of the stirring box and rotates with the top wall of the stirring box; the side wall of the stirring box is connected to an input pipe for inputting raw materials; the controller is used to control the operation of the double-headed driving member, thereby driving the main bevel gear to rotate.

[0023] Beneficial effects: Raw materials are input into the mixing box through the input pipe; the double-headed driving member is started by the controller, and one of the output shafts of the double-headed driving member will drive the main bevel gear to rotate, and then drive the secondary bevel gear to rotate, and the secondary bevel gear will drive the rotating shaft and the stirring rod to rotate, thereby uniformly stirring and mixing the raw materials in the mixing box.

[0024] Furthermore, the filter assembly includes a flexible tube connected to the bottom of the mixing box, and a control valve is connected to the connection point between the mixing box and the flexible tube; a filter screen is also fixedly connected in the flexible tube.

[0025] Beneficial effect: After the mixing and stirring of the raw materials is completed, a mixed solution is obtained, and the control valve is opened by the controller to allow the mixed solution to flow from the flexible pipe to the filter screen, thereby achieving preliminary filtration of the mixed solution.

[0026] Furthermore, the oscillation component includes an oscillation box, the bottom of which is connected to a solenoid valve, and the controller is used to control the opening and closing of the solenoid valve, thereby controlling the discharge of materials; the flexible tube is connected to the oscillation box at one end away from the mixing box; a sliding plate is fixedly connected to the top of the oscillation box; the oscillation box and the sliding plate both slide in cooperation with the inner wall of the outer shell; the bottom of the first bracket is fixedly connected to a base plate, and the bottom of the base plate is fixedly connected to a number of tension springs, and the bottoms of the tension springs are fixedly connected to the sliding plate; a driving component for pushing the sliding plate to move is provided on the top of the sliding plate.

[0027] Beneficial Effects: After preliminary filtration, the mixed solution flows into the oscillation box. The drive assembly pushes the sliding plate downward. At this point, as the sliding plate and the bottom plate move away from each other, the tension spring is stretched. When the sliding plate loses the restraining effect of the drive assembly, the tension spring pulls the sliding plate upward, causing the sliding plate to repeatedly move up and down along the inner wall of the shell, thereby driving the oscillation box up and down along the inner wall of the shell. This continuously oscillates the mixed solution in the oscillation box, facilitating the separation of the hydrogel and other impurities in the mixed solution. Heavier impurities fall to the bottom of the oscillation box. To extract the hydrogel, simply open the solenoid valve to discharge the impurities. Once transparent hydrogel flows out, the impurities are completely removed, effectively improving the quality of the finished product. The extracted hydrogel is then dialyzed and filtered to remove unreacted monomers, initiators, and small molecule impurities, thereby improving the purity of the hydrogel.

[0028] The cam is fixedly connected to the side panel of the second cam and the side panel that is located adjacent to the gear train of the second gear train, and the cam is fixedly connected to the side panel of the second cam and the side panel that is located adjacent to the gear train of the second gear train.

[0029] When the lever is in the unlocked position, the spring pulls the lever back into position, and the spring pulls the lever back into position, which in turn pushes the lever back into position, and the lever is in the unlocked position, which in turn pushes the lever back into position, and the lever is in the unlocked position, which in turn pushes the lever back into position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position, and the lever is in the unlocked position,

[0030] Furthermore, heating plates are embedded in the inner walls of the mixing box and the oscillation box, and the controller is used to adjust the heating temperature of the heating plates.

[0031] Beneficial effects: The heating temperature of the heating plate can be adjusted through the controller, and the temperature in the stirring box and the shaking box during the preparation process can be quickly adjusted, and the operation is simple.

[0032] Furthermore, a multifunctional hydrogel is used, the wound healing hydrogel is used to assist hemostasis, assist wound healing and assist tissue remodeling.

[0033] Beneficial effects: Applying the wound healing hydrogel to the wound surface can effectively stop bleeding, assist wound healing, and assist tissue remodeling.

[0034] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 Schematic diagram of the preparation method of the multifunctional hydrogel of the present invention.

[0036] Figure 2 It is a side cross-sectional view of the multifunctional hydrogel and the preparation device in the preparation method thereof of the present invention.

[0037] Figure 3 This is a front view of the drive assembly in the preparation device of the present invention.

[0038] Figure 4 This is a front cross-sectional view of the drive assembly in the preparation device of the present invention.

[0039] Figure 5 An axonometric view of the drive assembly in the device for preparing the present invention.

[0040] The figure marks in the drawings of the specification include: 1. outer shell; 2. first bracket; 3. mixing box; 4. main bevel gear; 5. secondary bevel gear; 6. rotating shaft; 7. stirring rod; 8. input pipe; 9. flexible pipe; 10. control valve; 11. filter screen; 12. vibration box; 13. sliding plate; 14. bottom plate; 15. tension spring; 16. turntable; 17. push rod; 18. second bracket; 19. push rod; 20. third bracket; 21. center rod; 22. hinged rod; 23. ratchet rod; 24. ratchet; 25. extension rod; 26. spring. DETAILED DESCRIPTION

[0041] The following is further described in detail through specific implementation methods:

[0042] Example 1:

[0043] like Figure 1 As shown, a multifunctional hydrogel, including a callus hydrogel, is composed of the following components: 20 parts acrylamide monomer, 5 parts hydrolyzed elastin, 1 part crosslinker MBA, and 1 part initiator APS. In this embodiment, the crosslinker MBA is N,N-methylenebisacrylamide, and the initiator APS is ammonium persulfate.

[0044] The preparation method of wound healing hydrogel is as follows:

[0045] S1. Preparation of acrylamide-hydrolyzed elastin mixed solution:

[0046] Prepare a preparation apparatus, weigh 2.6 g of acrylamide monomer and different mass fractions of hydrolyzed elastin, and dissolve them in deionized water; stir the mixture in the preparation apparatus at room temperature of 22°C for 30 minutes to dissolve the acrylamide and hydrolyzed elastin, thereby obtaining a mixed solution of acrylamide and hydrolyzed elastin;

[0047] S2, addition of initiator and crosslinker:

[0048] The initiator APS was added to the mixed solution prepared in S1, and stirred for 15 minutes using a preparation device at room temperature of 26°C to mix and dissolve the initiator APS and the mixed solution; then the cross-linker MBA was added to the mixed solution, and stirred for another 15 minutes using a preparation device at room temperature of 26°C; finally, the dissolved mixed solution was shaken for 5-10 minutes using a preparation device.

[0049] S3, Preparation of Callus Hydrogel:

[0050] At room temperature of 26°C, catalyst TMEDA was added to the mixed solution prepared by S2, and stirred for 15 minutes using a preparation device; the mixed solution was poured into a polytetrafluoroethylene mold, placed at room temperature of 26°C to react for 120 minutes to obtain a callus hydrogel; and the callus hydrogel was rinsed with deionized water for 35 minutes.

[0051] Wound healing hydrogels are used to assist hemostasis, wound healing, and tissue remodeling.

[0052] The specific implementation process is as follows:

[0053] Applying the healing hydrogel evenly on the wound surface can effectively stop bleeding, assist wound healing, and assist tissue remodeling.

[0054] This method optimizes the preparation formula of existing hydrogels. Unlike the existing technology, this method not only maintains the basic physical and chemical properties of the hydrogel by combining acrylamide with hydrolyzed elastin in different mass ratios, but also introduces the biocompatibility and bioactivity of elastin, so that the hydrogel can better fit the user's skin and have better skin-friendliness. The addition of hydrolyzed elastin may enhance the elasticity, flexibility and cell affinity of the hydrogel, so that the hydrogel prepared by this method can better act on wound healing. By establishing a diabetic rat infected wound model and evaluating the effect of the hydrogel prepared by the present invention, the following conclusions were drawn: The hydrogel prepared by the present invention promotes the healing of diabetic wounds. The results showed that the hydrogel had efficient antibacterial activity, effectively reduced the level of oxidative stress, controlled infection, accelerated angiogenesis, and promoted angiogenesis, thereby achieving rapid healing of diabetic wounds.

[0055] This method promotes effective crosslinking between monomers by adding the crosslinking agent MBA and the initiator APS during the preparation process, and by stirring and ultrasonicating under appropriate conditions. This results in a stable three-dimensional network structure, which in turn helps improve the mechanical strength and durability of the hydrogel. Scanning electron microscopy (SEM) observations show that the hydrogel has a porous structure, which facilitates the exchange of nutrients and metabolites between the hydrogel dressing and the wound.

[0056] Example 2:

[0057] like Figure 2-Figure 5 As shown, different from the above embodiments, the preparation device includes a controller and a housing 1, and the housing 1 is provided with a stirring component for stirring the mixed solution, a filtering component for filtering the mixed solution, and a shaking component for shaking the mixed solution.

[0058] like Figure 2 As shown, the stirring assembly includes a first bracket 2 and a stirring box 3 welded to the outer shell 1, and a double-headed driving member is embedded in the first bracket 2; a main bevel gear 4 is fixedly connected to the right output shaft of the double-headed driving member with a coaxial bolt, and the main bevel gear 4 is engaged with a secondary bevel gear 5, and a rotating shaft 6 is fixedly connected to the bottom of the secondary bevel gear 5 with a coaxial bolt, and a number of stirring rods 7 are fixedly connected to the rotating shaft 6 with bolts; the bottom of the rotating shaft 6 extends to the inside of the stirring box 3 and rotates with the top wall of the stirring box 3; the side wall of the stirring box 3 is connected to an input pipe 8 for inputting raw materials; the controller is used to control the operation of the double-headed driving member, thereby driving the main bevel gear 4 to rotate.

[0059] like Figure 2 As shown, the filter assembly includes a flexible tube 9 connected to the bottom of the mixing box 3, and a control valve 10 is connected to the connection point between the mixing box 3 and the flexible tube 9; a filter screen 11 is also fixedly bonded and connected to the flexible tube 9.

[0060] like Figure 2 As shown, the oscillation component includes an oscillation box 12, the bottom of which is connected to a solenoid valve, and the controller is used to control the opening and closing of the solenoid valve, thereby controlling the discharge; the bottom end of the flexible tube 9 is connected to the oscillation box 12; the top of the oscillation box 12 is fixedly connected with a sliding plate 13 by bolts; the oscillation box 12 and the sliding plate 13 are both slidably matched with the inner wall of the outer shell 1; a bottom plate 14 is welded to the bottom of the first bracket 2, and a number of tension springs 15 are fixedly connected with screws at the bottom of the bottom plate 14, and the bottoms of the tension springs 15 are all fixedly connected with screws to the sliding plate 13; a driving component for pushing the sliding plate 13 to move is provided at the top of the sliding plate 13.

[0061] like Figure 2-Figure 5As shown, the drive assembly includes a turntable 16, which is coaxially bolted to the left output shaft of the double-headed drive member; a plurality of push rods 17 are bolted to the left side of the turntable 16; a second bracket 18 is also bolted to the top of the base plate 14; a center rod 21 is rotatably engaged with the second bracket 18, and a hinged rod 22 is rotatably engaged with the side of the center rod 21 near the turntable 16. A ratchet 23 is hingedly connected to the end of the hinged rod 22 away from the center rod 21; a ratchet 24 is bolted to the end of the center rod 21 near the turntable 16, and the ratchet 23 engages with the ratchet 24; an extension rod 25 is bolted to the left side of the ratchet rod 23, and a spring 26 is screwed to the left side of the extension rod 25. The end of the spring 26 away from the extension rod 25 is screwed to the side wall of the second bracket 18. The design of the extension rod 25 provides a good installation position for the spring 26, avoiding interference with other components.

[0062] A third bracket 20 for providing a fulcrum for the hinged rod 22 is also bolted to the top of the bottom plate 14 ; a push rod 19 for pushing the sliding plate 13 is bolted to the side of the center rod 21 away from the hinged rod 22 along its circumference.

[0063] In this embodiment, the double-headed driving member is a double-headed motor.

[0064] The specific implementation process is as follows:

[0065] by Figure 2 For example, the operator inputs raw materials into the mixing box 3 through the input pipe 8; the double-headed motor is started by the controller, and the output shaft on the right side of the double-headed motor drives the main bevel gear 4 to rotate. Since the main bevel gear 4 is engaged with the sub-bevel gear 5, the main bevel gear 4 drives the sub-bevel gear 5 to rotate, and the sub-bevel gear 5 drives the rotating shaft 6 and the stirring rod 7 to rotate, thereby uniformly stirring and mixing the raw materials in the mixing box 3.

[0066] When the mixing and stirring of the raw materials is completed, a mixed solution is obtained. The control valve 10 is opened by the controller, so that the mixed solution flows from the flexible tube 9 to the filter 11. The filter 11 will filter the impurities in the mixed solution, thereby achieving preliminary filtration of the mixed solution; after the preliminary filtration, the mixed solution will flow into the shock box 12.

[0067] by Figure 2 and Figure 4For example, at the same time, keeping the double-headed motor running, the left output shaft of the double-headed motor will drive the turntable 16 to rotate counterclockwise, and then drive the push rod 17 to rotate counterclockwise, and the push rod 17 will contact the bottom of the ratchet rod 23 and push the ratchet rod 23 and the hinged rod 22 to move upward. Since the ratchet rod 23 is engaged with the ratchet wheel 24, the ratchet rod 23 will push the ratchet wheel 24 to rotate; when the push rod 17 continues to rotate, the push rod 17 will be disengaged from the bottom of the ratchet rod 23, and the ratchet rod 23 loses the limit of the push rod 17. Due to the tension of the spring 26 and the weight of the ratchet rod 23 itself, the ratchet rod 23 will quickly fall back to its original position and drive the hinged rod 22 back to the third bracket 20. The top; thus, the ratchet 24 will rotate intermittently, thereby driving the center rod 21 to rotate intermittently; the center rod 21 will drive the push rod 19 to rotate intermittently, thereby causing the push rod 19 to continuously push the sliding plate 13 to move downward, and the tension spring 15 will repeatedly pull the sliding plate 13 back upward, and the intermittent rotation of the push rod 19 allows the tension spring 15 to completely restore to its initial state. This reciprocating motion can maximize the oscillation distance of the oscillation box 12, thereby maximizing the oscillation of the mixed solution in the oscillation box 12, thereby allowing the mixed solution in the oscillation box 12 to be better stratified, thereby improving the quality of the finished product.

[0068] In the initial state, the tension spring 15 is in a compressed state, and the top of the vibration box 12 is almost in contact with the bottom of the mixing box 3, so that the vibration box 12 can move to the maximum extent; and since the flexible tube 9 is a hose, it will not affect the movement of the vibration box 12.

[0069] The width of the sliding plate 13 is smaller than the maximum gap between adjacent push rods 19 , so that when the sliding plate 13 moves upward, it enters the gap between adjacent push rods 19 , and then moves downward after being squeezed by the push rods 19 .

[0070] by Figure 2 For example, in this process, when the push rod 19 pushes the sliding plate 13 to move downward, the sliding plate 13 and the bottom plate 14 move away from each other, and the tension spring 15 will be stretched. When the sliding plate 13 loses the limiting effect of the push rod 19, the tension spring 15 will pull the sliding plate 13 upward, and so on. As a result, the sliding plate 13 moves up and down repeatedly along the inner wall of the outer shell 1, thereby driving the shock box 12 to move up and down along the inner wall of the outer shell 1, thereby realizing continuous oscillation of the mixed solution in the shock box 12, which is beneficial to the stratification of hydrogel and other impurities in the mixed solution. The heavier impurities will fall to the bottom of the shock box 12. When extracting the hydrogel, you only need to open the solenoid valve to discharge the impurities. Until transparent and clear hydrogel flows out, it means that the impurities are completely discharged. Then the hydrogel in the shock box 12 at this time is extracted, and the hydrogel is further ultrasonically treated to further optimize the quality of the finished product.

[0071] The design of the preparation device can greatly improve the preparation efficiency; the preparation device can simultaneously complete multiple steps such as stirring, mixing, shaking, filtering and temperature adjustment of multiple raw materials, without the need for operators to frequently change preparation equipment, and the operation is simple.

[0072] It is simple and can effectively improve the preparation efficiency of hydrogels; and it integrates multiple functions into a set of equipment, eliminating the need to use multiple devices and effectively reducing the preparation cost of hydrogels.

[0073] Example 3:

[0074] Different from the above embodiment, heating plates (not shown in the figure) are embedded in the inner walls of the stirring box 3 and the oscillation box 12, and the controller is used to adjust the heating temperature of the heating plates.

[0075] The specific implementation process is as follows: the operator can adjust the heating temperature of the heating plate through the controller, and then quickly adjust the temperature in the stirring box 3 and the shaking box 12 during the preparation process. The operation is simple and can effectively improve the preparation efficiency.

[0076] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A multifunctional hydrogel, characterized in that: The wound healing hydrogel comprises the following components: 10-20 parts of acrylamide monomer, 1-5 parts of hydrolyzed elastin, 1-2 parts of cross-linking agent MBA and 1-2 parts of initiator APS.

2. A method for preparing a multifunctional hydrogel, based on the multifunctional hydrogel of claim 1, characterized in that: The following steps are involved: S1. Preparation of acrylamide-hydrolyzed elastin mixed solution: Prepare a preparation apparatus, weigh 2.6 g of acrylamide monomer and different mass fractions of hydrolyzed elastin, and dissolve them in deionized water; stir the mixture in the preparation apparatus at room temperature of 22-26°C for 25-30 minutes to dissolve the acrylamide and hydrolyzed elastin, thereby obtaining a mixed solution of acrylamide and hydrolyzed elastin; S2, addition of initiator and crosslinker: Add the initiator APS to the mixed solution prepared in S1, and stir for 15 minutes using a preparation device at room temperature of 22-26°C to mix and dissolve the initiator APS and the mixed solution; Then, the crosslinking agent MBA was added to the mixed solution, and the mixture was stirred for 15 minutes at room temperature of 22-26°C using the preparation device; finally, the dissolved mixed solution was shaken for 5-10 minutes using the preparation device; S3, Preparation of Callus Hydrogel: At room temperature of 22-26°C, catalyst TMEDA was added to the mixed solution prepared in S2, and the mixture was stirred for 15 minutes using a preparation device; the mixed solution was poured into a polytetrafluoroethylene mold, and the mixture was allowed to react at room temperature of 22-26°C for 90-120 minutes to obtain a callus hydrogel; the callus hydrogel was rinsed with deionized water for 30-35 minutes.

3. The method for preparing the multifunctional hydrogel according to claim 2, wherein: In S1, the mass fraction of acrylamide is 26%, and the mass fractions of hydrolyzed elastin include 0.5%, 1.0%, 2.0% and 3.0%.

4. The method for preparing the multifunctional hydrogel according to claim 3, wherein: In S2, the power of the ultrasonic equipment is controlled at 200-300w, and the ultrasonic treatment is carried out for 25-30min.

5. The method for preparing the multifunctional hydrogel according to claim 4, wherein: In S1, the preparation device includes a controller and a housing (1); The housing (1) is provided with a stirring component for stirring the mixed solution, a filtering component for filtering the mixed solution, and a shaking component for shaking the mixed solution; The stirring assembly comprises a first bracket (2) fixedly connected to the housing (1) and a stirring box (3); a double-headed driving member is embedded and installed on the first bracket (2); a main bevel gear (4) is coaxially fixedly connected to one of the output shafts of the double-headed driving member, the main bevel gear (4) is meshed with a secondary bevel gear (5), a rotating shaft (6) is coaxially fixedly connected to the bottom of the secondary bevel gear (5), and a plurality of stirring rods (7) are fixedly connected to the rotating shaft (6); the bottom of the rotating shaft (6) extends into the interior of the stirring box (3) and is rotatably engaged with the top wall of the stirring box (3); The side wall of the mixing box (3) is connected to an input pipe (8) for inputting raw materials; The controller is used to control the operation of the double-head driving member, thereby driving the main bevel gear (4) to rotate.

6. The method for preparing the multifunctional hydrogel according to claim 5, wherein: The filter assembly comprises a flexible pipe (9) connected to the bottom of the mixing box (3); a control valve (10) is connected to the connection point between the mixing box (3) and the flexible pipe (9); and a filter screen (11) is fixedly connected in the flexible pipe (9).

7. The method for preparing the multifunctional hydrogel according to claim 6, wherein: The oscillation component includes an oscillation box (12), the bottom of the oscillation box (12) is connected to a solenoid valve, and the controller is used to control the opening and closing of the solenoid valve, thereby controlling the discharge of materials; One end of the flexible tube (9) away from the stirring box (3) is connected to the vibration box (12); The top of the oscillation box (12) is fixedly connected to a sliding plate (13); the oscillation box (12) and the sliding plate (13) are both slidably matched with the inner wall of the housing (1); the bottom of the first bracket (2) is fixedly connected to a bottom plate (14), the bottom of the bottom plate (14) is fixedly connected to a plurality of tension springs (15), and the bottoms of the tension springs (15) are all fixedly connected to the sliding plate (13); A driving assembly for pushing the sliding plate (13) to move is provided on the top of the sliding plate (13).

8. The method for preparing the multifunctional hydrogel according to claim 7, wherein: The driving assembly includes a turntable (16), which is coaxially fixedly connected to the output shaft of the double-headed driving member at one end away from the main bevel gear (4); a plurality of push rods (17) are fixedly connected to the side of the turntable (16) away from the double-headed driving member; The top of the bottom plate (14) is also fixedly connected to a second bracket (18); a center rod (21) is rotatably engaged with the second bracket (18); a hinged rod (22) is rotatably engaged with the side of the center rod (21) close to the turntable (16); a ratchet (23) is hingedly connected to the end of the hinged rod (22) away from the center rod (21); a ratchet (24) is fixedly connected to the end of the center rod (21) close to the turntable (16), and the ratchet (23) is meshed with the ratchet (24); The side of the ratchet rod (23) away from the rotating disk (16) is fixedly connected to the extension rod (25), the side of the extension rod (25) away from the ratchet rod (23) is fixedly connected to the spring (26), and the end of the spring (26) away from the extension rod (25) is fixedly connected to the side wall of the second bracket (18); A third bracket (20) is also fixedly connected to the top of the bottom plate (14) and is used to provide a fulcrum for the hinged rod (22); A push rod (19) for pushing the sliding plate (13) is fixedly connected along the circumference of the central rod (21) on one side away from the hinge rod (22).

9. The method for preparing the multifunctional hydrogel according to claim 8, wherein: Heating plates are embedded in the inner walls of the stirring box (3) and the oscillation box (12), and the controller is used to adjust the heating temperature of the heating plates.

10. An application of a multifunctional hydrogel, based on the multifunctional hydrogel of claim 1, characterized in that: Wound healing hydrogels are used to assist hemostasis, wound healing, and tissue remodeling.

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